FR2403556A1 - Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support - Google Patents

Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support

Info

Publication number
FR2403556A1
FR2403556A1 FR7728161A FR7728161A FR2403556A1 FR 2403556 A1 FR2403556 A1 FR 2403556A1 FR 7728161 A FR7728161 A FR 7728161A FR 7728161 A FR7728161 A FR 7728161A FR 2403556 A1 FR2403556 A1 FR 2403556A1
Authority
FR
France
Prior art keywords
antibody
virus
support
porous mineral
mineral support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7728161A
Other languages
French (fr)
Other versions
FR2403556B1 (en
Inventor
Michel Tardy
Jean-Louis Tayot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Merieux SA
Original Assignee
Institut Merieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Merieux SA filed Critical Institut Merieux SA
Priority to FR7728161A priority Critical patent/FR2403556A1/en
Publication of FR2403556A1 publication Critical patent/FR2403556A1/en
Application granted granted Critical
Publication of FR2403556B1 publication Critical patent/FR2403556B1/fr
Granted legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28057—Surface area, e.g. B.E.T specific surface area
    • B01J20/28059—Surface area, e.g. B.E.T specific surface area being less than 100 m2/g
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
    • B01J20/28078—Pore diameter
    • B01J20/28085—Pore diameter being more than 50 nm, i.e. macropores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/3092—Packing of a container, e.g. packing a cartridge or column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • B01J20/3282—Crosslinked polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column

Abstract

Solid porous materials for use in chromatography columns comprise a porous mineral support coated directly with an adsorbed antibody layer stabilised by crosslinking. The support can be a refractory oxide (e.g. SiO2, Al2O3, MgO or TiO2) or a glass, silicate, borosilicate or kaolin. The antibody is pref. an antibody to cholera toxin, but can also be an antibody to diphtheria toxin, tetanus toxin, virus HB, influenza, virus, hepres virus, alpha-foetoprotein, gamma-globulin, albumin, etc. The materials are esp. useful for affinity-chromatographic isolation of cholera toxin, e.g. for vaccin prodn. The antibody is strongly bound to the support without the use of intermediate layers of polymers or coupling agents.
FR7728161A 1977-09-19 1977-09-19 Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support Granted FR2403556A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7728161A FR2403556A1 (en) 1977-09-19 1977-09-19 Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7728161A FR2403556A1 (en) 1977-09-19 1977-09-19 Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support

Publications (2)

Publication Number Publication Date
FR2403556A1 true FR2403556A1 (en) 1979-04-13
FR2403556B1 FR2403556B1 (en) 1982-04-23

Family

ID=9195520

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7728161A Granted FR2403556A1 (en) 1977-09-19 1977-09-19 Affinity chromatographic substrates - with antibody crosslinked directly on porous mineral support

Country Status (1)

Country Link
FR (1) FR2403556A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982002818A1 (en) * 1981-02-26 1982-09-02 Gani Mohamed Mutwahar A process and apparatus for the recovery of immunoglobulins
EP0161107A2 (en) * 1984-05-08 1985-11-13 Farmos-Yhtyma Oy Immunometric method for the determination of a hapten
DE3627063A1 (en) * 1986-08-09 1988-02-11 Diagen Inst Molekularbio METHOD FOR SEPARATING AND CLEANING BIOPOLYMERS BY AFFINITY CHROMATOGRAPHY

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1148574A (en) * 1965-03-26 1969-04-16 Mount Sinai Hospital Res Found Molecular sieve coated particulate adsorbent and processes using same
GB1387625A (en) * 1971-04-23 1975-03-19 Secr Defence Immunospecific separation of antigens and antibodies
FR2319399A1 (en) * 1975-07-29 1977-02-25 Merieux Inst NEW MATERIAL WITH CATIONIC CHARACTER, CAPABLE OF REVERSIBLE FIXING, BIOLOGICAL MACROMOLECULES, PREPARATION METHODS AND APPLICATIONS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1148574A (en) * 1965-03-26 1969-04-16 Mount Sinai Hospital Res Found Molecular sieve coated particulate adsorbent and processes using same
GB1387625A (en) * 1971-04-23 1975-03-19 Secr Defence Immunospecific separation of antigens and antibodies
FR2319399A1 (en) * 1975-07-29 1977-02-25 Merieux Inst NEW MATERIAL WITH CATIONIC CHARACTER, CAPABLE OF REVERSIBLE FIXING, BIOLOGICAL MACROMOLECULES, PREPARATION METHODS AND APPLICATIONS

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982002818A1 (en) * 1981-02-26 1982-09-02 Gani Mohamed Mutwahar A process and apparatus for the recovery of immunoglobulins
EP0059598A1 (en) * 1981-02-26 1982-09-08 Unilever Plc A process and apparatus for the recovery of immunoglobulines
US4490290A (en) * 1981-02-26 1984-12-25 Internationale Octrooi Maatschappij "Octropa" B.V. Process for the recovery of immunoglobulins
EP0161107A2 (en) * 1984-05-08 1985-11-13 Farmos-Yhtyma Oy Immunometric method for the determination of a hapten
EP0161107A3 (en) * 1984-05-08 1986-12-10 Farmos-Yhtyma Oy Immunometric method for the determination of a hapten
DE3627063A1 (en) * 1986-08-09 1988-02-11 Diagen Inst Molekularbio METHOD FOR SEPARATING AND CLEANING BIOPOLYMERS BY AFFINITY CHROMATOGRAPHY
EP0263934A1 (en) * 1986-08-09 1988-04-20 DIAGEN Institut für molekularbiologische Diagnostik GmbH Method for separation and purification of biopolymers by affinity chromatography

Also Published As

Publication number Publication date
FR2403556B1 (en) 1982-04-23

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