FI98765C - Flödescytometrisk metod och anordning - Google Patents

Flödescytometrisk metod och anordning Download PDF

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Publication number
FI98765C
FI98765C FI950174A FI950174A FI98765C FI 98765 C FI98765 C FI 98765C FI 950174 A FI950174 A FI 950174A FI 950174 A FI950174 A FI 950174A FI 98765 C FI98765 C FI 98765C
Authority
FI
Finland
Prior art keywords
excitation
cells
photon
fluorescence
flow
Prior art date
Application number
FI950174A
Other languages
English (en)
Finnish (fi)
Other versions
FI950174A (sv
FI950174A0 (sv
FI98765B (sv
Inventor
Pekka Haenninen
Erkki Soini
Original Assignee
Erkki Soini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8542323&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI98765(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Publication of FI950174A0 publication Critical patent/FI950174A0/sv
Priority to FI950174A priority Critical patent/FI98765C/sv
Application filed by Erkki Soini filed Critical Erkki Soini
Priority to EP96900107A priority patent/EP0804723B1/en
Priority to PCT/FI1996/000003 priority patent/WO1996022521A1/en
Priority to JP8522061A priority patent/JPH10512952A/ja
Priority to US08/817,797 priority patent/US6177277B1/en
Priority to DE69628542T priority patent/DE69628542T2/de
Publication of FI950174A publication Critical patent/FI950174A/sv
Publication of FI98765B publication Critical patent/FI98765B/sv
Application granted granted Critical
Publication of FI98765C publication Critical patent/FI98765C/sv
Priority to JP2004226683A priority patent/JP2004347608A/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream

Claims (4)

1. Flödescytometrisk metod för bestämning av partiklar i biologiska prov kännetecknad därav, att partikeln behandlas med ett för densamma specifikt fluorescerande färgämne och att fluorescensens excitering utförs som tväfoton- 5 excitering.
2. Metod enligt patentkravet 1 kännetecknad därav, att man med hjälp av därav detekterar partiklar, som förekommer säsom synnerligen sällsynta i biologiska prov.
3. Flödescytometer avsedd för metoden enligt patentkravet 1 10 kännetecknad därav, att den är försedd med ett optiskt system, som lämpar sig för tväfotonexcitering av fluorescensen.
4. Flödescytometer enligt patentkravet 3 kännetecknad därav, att det optiska systemet är konfokalt.
FI950174A 1995-01-16 1995-01-16 Flödescytometrisk metod och anordning FI98765C (sv)

Priority Applications (7)

Application Number Priority Date Filing Date Title
FI950174A FI98765C (sv) 1995-01-16 1995-01-16 Flödescytometrisk metod och anordning
EP96900107A EP0804723B1 (en) 1995-01-16 1996-01-03 A flow fluorometric method and device
DE69628542T DE69628542T2 (de) 1995-01-16 1996-01-03 Verfahren und vorrichtung zur durchflusscytometrie
PCT/FI1996/000003 WO1996022521A1 (en) 1995-01-16 1996-01-03 A flow fluorometric method and device
JP8522061A JPH10512952A (ja) 1995-01-16 1996-01-03 フロー蛍光法及び装置
US08/817,797 US6177277B1 (en) 1995-01-16 1996-01-03 Flow fluorometric method
JP2004226683A JP2004347608A (ja) 1995-01-16 2004-08-03 フロー蛍光法及び装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI950174 1995-01-16
FI950174A FI98765C (sv) 1995-01-16 1995-01-16 Flödescytometrisk metod och anordning

Publications (4)

Publication Number Publication Date
FI950174A0 FI950174A0 (sv) 1995-01-16
FI950174A FI950174A (sv) 1996-07-17
FI98765B FI98765B (sv) 1997-04-30
FI98765C true FI98765C (sv) 1997-08-11

Family

ID=8542323

Family Applications (1)

Application Number Title Priority Date Filing Date
FI950174A FI98765C (sv) 1995-01-16 1995-01-16 Flödescytometrisk metod och anordning

Country Status (6)

Country Link
US (1) US6177277B1 (sv)
EP (1) EP0804723B1 (sv)
JP (2) JPH10512952A (sv)
DE (1) DE69628542T2 (sv)
FI (1) FI98765C (sv)
WO (1) WO1996022521A1 (sv)

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Also Published As

Publication number Publication date
JPH10512952A (ja) 1998-12-08
DE69628542T2 (de) 2003-12-18
FI950174A (sv) 1996-07-17
FI950174A0 (sv) 1995-01-16
JP2004347608A (ja) 2004-12-09
US6177277B1 (en) 2001-01-23
DE69628542D1 (de) 2003-07-10
EP0804723A1 (en) 1997-11-05
WO1996022521A1 (en) 1996-07-25
FI98765B (sv) 1997-04-30
EP0804723B1 (en) 2003-06-04

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Owner name: SOINI, ERKKI

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