ES2791285T3 - Sample collection kit and procedure for the collection of plant samples, in particular algae samples - Google Patents

Abstract

Sample collection kit (100), which is configured for the collection of plant samples, in particular algae samples for biomedical applications, comprising: - at least one sample container (10) which is configured to house plant samples, - an array of sensors with several sensors (21, 22, ...) with which sample data can be taken, - a data logger (30) which is configured to record the sample data, - a set of tools with collection tools (41, 42, ...) which are configured for a collection of plant samples and with processing tools (51, 52, ...) which are configured for a processing of plant samples, - a set of devices with auxiliary devices (61, 62, ...) which can be used in the collection, transport and processing of plant samples and which contain auxiliary devices for func electrical ionization, and - a container set (70) that is configured to house the set of tools and the set of devices in a state isolated from the environment, characterized by - identification marks (80) which contain data from identification and that are applied in at least one sample container, the collection tools (41, 42, ...) and the processing tools (51, 52, ...), where - the container equipment (70 ) is equipped with a charging interface (74), through which the auxiliary devices for electrical operation of the set of devices can be supplied with electric charging current.

Description

DESCRIPTION

Sample collection kit and procedure for the collection of plant samples, in particular algae samples

The invention relates to a sample collection kit which is configured for the collection of plant samples, in particular for the collection of algae samples for biomedical applications. The invention further relates to a method for the collection of plant samples using the sample collection kit. The applications of the invention are in the production of plant extracts, in particular for biomedical applications, for example, in the production of alginate granules.

Alginate is a polysaccharide obtained from seaweed and has numerous applications in chemical engineering, food technology, and biomedicine. In biomedicine, alginate is used, for example, to make implants. In implants for medical applications, for example for use in human implant recipients, high demands are placed on biofunctionality (in particular biocompatibility), cleanliness and stability. To meet these requirements, it is generally known how to subject alginates to predetermined cleaning procedures to minimize fluctuations in composition. In addition, the patent DE 102005040872 B3 knows how to document the collection place (geographical location of the algae collection) and sample data of the collection place and the transport of the algae to the consumer to minimize fluctuations in the biofunctionality of the algae. alginates obtained from algae.

So far, algae harvesting has been done manually by fishermen using reusable tools. The fishermen also carry out a first treatment of the algae collected and, if necessary, a drying. After harvesting, the harvested algae are sent to the consumer in a sample container, which can be equipped with sensors and a data logger, for example according to DE 102005040872 B3, from which alginate from algae is obtained dry, for example for the production of an implant.

In practice, however, it has been shown that alginates, even if their collection and transport conditions are documented, can present undesirable fluctuations in their biofunctionality. Furthermore, the approval of plant products for medical applications is subject to strict conditions, in particular guidelines to ensure the quality of production processes and the environment (eg GMP, "Good Manufacturing Practices"), which are difficult due to to the preconditions of collection and transport, and can be met only with reduced reliability.

The aforementioned problems occur not only in the collection of algae and the obtaining of alginate, but also in the collection of other plant samples, for example, for the production of active substances.

Sample collection and test boxes are known, for example from CN 201 724 839 U (utility model) and WO 2009/143339.

The object of the invention is to provide an improved sample collection kit and an improved procedure for the collection of plant samples, in particular algal samples, thereby avoiding the disadvantages of conventional techniques. The sample collection kit and the procedure are intended in particular to allow the obtaining of alginate with greater reliability and reproducibility of the collection and processing of the algae, greater reproducibility of the composition and / or reduced fluctuations in biofunctionality. Furthermore, compliance with quality assurance standards, such as GMP standards, should be improved in particular.

These objectives are achieved by means of a sample collection kit and a procedure for the collection of plant samples with the characteristics of the independent claims. Advantageous embodiments and applications of the invention appear from the dependent claims.

According to a first general aspect of the invention, the aforementioned objective is achieved by a sample collection kit which is configured for the collection of plant samples, in particular algae samples, and which contains a sensor arrangement with several sensors to record sample data, working media for primary collection (collection), transportation and processing of plant samples, for example to obtain a plant extract, and identification marks with identification data of working media.

The sample collection kit is advantageously suitable for all phases, from collecting the plant samples to obtaining the plant extract. Preferably, all the working means, in particular auxiliary tools and apparatus, which are required until the plant extract is produced, are included in the sample collection kit and at least the tools can be identified by means of an identification mark. This allows the plant extract to be provided along with sample data and identification data, allowing a complete characterization of the plant extract collection and production procedure. In addition, the individual stages of collecting, transporting and processing plant samples are unified through the provision of work equipment, so that fluctuations in the properties of the collected plant samples and the plant extract produced or of the substances obtained from them, in particular fluctuations in biofunctionality. If contamination or errors are found when using the plant samples obtained with the sample collection kit, their causes can be traced back to the collection of the plant samples based on the complete sample data and identification data.

The sample collection kit generally represents a set of working means for collecting, transporting and processing plant samples. In particular, the sample collection kit comprises at least one sample container for housing the plant samples. The sample container is preferably configured for the liquid-tight accommodation of plant samples and comprises, for example, a bag made of a plastic foil. The sample container advantageously offers the maintenance of a humid environment and a protection against the penetration of unwanted foreign substances during transport. Furthermore, the sample collection kit comprises a sensor arrangement with various sensors with which sample data representing properties of the collected plant samples or the type of sample collection can be collected, in particular the physical properties and / or or chemicals of the plant samples and / or the physical and / or chemical conditions of the collection, transport and processing of the plant sample, and a data recording equipment (with at least one data recorder), which is configured to record sample data. The data logging equipment may comprise a data logger, which is fixedly connected to the sample collection kit, and one or more data loggers, which are provided for connection to sample containers in the sample collection kit. samples to record data during transport.

The sample collection kit advantageously comprises a tool kit containing collection tools and processing tools. The collection tools are adapted for a collection of plant samples. Advantageously, the user, for example as when collecting algae, the fisherman, has uniform collection tools, whereby the conditions for collecting the plant samples are unified. The processing tools are adapted for processing, in particular obtaining extracts from the plant samples in a processing site separate from the natural collection site, in particular in a processing laboratory. The processing tools comprise working means and utensils for use by the user of the sample collection kit that act on the plant samples during processing. When specifying the processing tools, as with the collection tools, there is the advantage of uniform and reproducible processing up to the obtaining of the plant extract. Preferably, the collection tools and the processing tools are disposable working media that are used once for the collection and processing of plant samples from a common lot.

Advantageously, the sample collection kit also comprises a set of apparatus with auxiliary devices that can be used in the collection, transport and processing of plant samples and, in particular, are configured to obtain documentation of the physical and / or chemical conditions. during these stages.

In addition, the sample collection kit comprises a container kit for housing the collection tools, the processing tools and the auxiliary apparatus in a state isolated from the environment. The container kit, which is preferably modular in construction, constitutes a package for the sample collection kit. Advantageously, the container equipment offers protection of the working means against the use of the sample collection kit and a defined arrangement of the working means, thus simplifying the use of the sample collection kit, for example by specifying a sequence of the sample collection. use of collection or processing tools.

According to a second general aspect of the invention, the above objective is achieved by a sample collection method for the collection of plant samples, in which the sample collection kit according to the first general aspect of the invention is used. The sample collection procedure comprises collecting the plant samples at a natural sample collection site using the collection tools, where the plant samples are moved to at least one sample container and sample data is collected. Next, the at least one sample container and the sample processing kit are transported to a processing laboratory, where during transport the sample data is complemented with data on the physical and / or chemical conditions of the transport. in particular the route of transport and the duration of transport. A plant extract is then produced in the processing laboratory using the processing tools. During processing, the sample data is supplemented with information on the physical and / or chemical conditions of the processing. Finally, the finished plant extract is provided together with a data set comprising the sample data and the identification data of the sample collection kit.

The invention advantageously improves the process of collecting and preparing plant samples, in particular algae, with respect to the purity of the plant extract and the documentation and traceability of the individual process steps. The inventors have verified the fluctuations in the biofunctionality of the vegetal samples obtained using conventional techniques, due to the contamination of the working media, possibly reused tools or with insufficient cleaning and undefined and insufficiently documented parameters of the general process (place of collection, collection time, transportation time, physical and / or chemical environmental parameters, etc.) and the use of non-uniform working means were affected. Advantageously, in contrast to conventional techniques, the sample collection kit simplifies the production of material with reproducible properties. An important advantage of the sample collection kit, achieved by the inventors, is that the sample collection kit can accommodate all the working means required for sample collection and processing, and even so, the sample collection kit it can be operated manually, in particular the user can carry it by the hand. This advantage is particularly important for obtaining alginate in extreme environmental conditions, for example, in the open sea or in the event of waves.

Preferably, the sample collection kit is a set of working means for the complete disposable use of tools and consumables. On the other hand, auxiliary devices, such as, for example, documentation devices, in particular satellite receivers, clocks, communication devices, lamps and sensors can be intended for single use or repeated use.

Other advantages of the invention consist in that the sample collection kit represents a defined initial state for the collection of plant samples and the use of the sample collection kit is associated with a saving of time for the user. The invention allows an unambiguous identification of the entire sample collection kit and of all work equipment and materials packed in it. This advantageously ensures complete traceability of all components of the kit. Another advantage of the sample collection kit is the increased safety for the user, since the user works with standard, new and tested tools and, optionally, the sample collection kit is equipped with an emergency system (communication device, kit first AIDS).

According to a preferred embodiment of the invention, the sample collection kit also contains consumables that can be used in the collection and processing of plant samples. Consumables include, for example, water, disinfectants and / or test substances, for example for the detection of foreign substances. By providing consumables with the sample collection kit, the reliability and reproducibility of the individual work steps is advantageously improved.

According to a further preferred embodiment of the sample collection kit, the container kit comprises a housing for the collection tools and the processing tools before the start of collection of the plant samples in a sterile state. In the container equipment, the collection tools and the processing tools are housed with a separation from the environment in terms of materials. The container equipment is preferably provided with a seal that is intended to be destroyed only at the beginning of harvest.

The container equipment of the sample collection kit is especially preferably constructed from several container modules comprising a collection module, a documentation module and a processing module. Each container module consists of a closed, separate container that contains all the working means for the individual phases of collection and processing and / or the auxiliary devices. The collection module is configured to house the collection tools. The collection module contains accommodation spaces that are adapted to the number, size and shape of the collection tools required. Inadvertent holding of unsuitable collecting tools is advantageously avoided. The processing module is configured to house the processing tools. Also in the case of processing tools, specific accommodation spaces are provided in the processing module, thus avoiding the use of unsuitable processing tools. The documentation module preferably contains the devices auxiliaries that are used when necessary in the collection and processing of plant samples.

According to the invention, the container equipment is equipped with a charging interface, through which the electrically operated auxiliary devices of the sample collection kit and optionally also the electrically operated tools can be supplied with a current electrical charge. Various possibilities are advantageously available for the supply of electric current. For example, a wireless inductive power supply or a wired power supply may be provided. The charging interface particularly preferably comprises a charging socket which is arranged on an outer side of the container equipment for receiving the plug of a charging cable. Advantageously, the sample collection kit can be electrically charged without having to open the container equipment, so that the sterility of the working means can be maintained during charging.

Furthermore, the container equipment can be equipped with a wireless or wired data interface, through which data exchange is carried out between the data recording equipment and an external control apparatus, for example an external computer.

According to a further advantageous embodiment of the invention, the collecting tools in the collecting module and the processing tools in the processing module can be arranged side by side in such a way that they form a row according to the order in which the respective tools are used for the collection and processing of plant samples. The collection module and the processing module provide the tools according to the development of the method according to the invention as a workflow, whereby the reliability and reproducibility of the use of the sample collection kit is advantageously improved.

Alternatively or additionally, the collection module may be equipped with a release kit that allows the collection module to open only at a predetermined geographic sample collection location and further locks a closure of the collection module. The release equipment and its operation can be carried out for example as described in DE 102005040872 B3.

Additional advantages are obtained for using the sample collection kit if the container modules are detachably connected to each other. For this purpose, the container modules are preferably equipped on their outer sides with matching connecting elements, for example retaining elements. The sample collection kit comprises, for example, a stack of assembled container modules. If the container modules have carrying handles, there are advantages to handling the sample collection kit and the individual container modules during the collection, transport and processing phases of the plant samples. The container modules are preferably of the same shape and size, so that combining the container modules to create sample collection kits for different tasks is simplified.

According to a further, particularly advantageous, embodiment of the invention, the container modules have, in each case, a holding platform with accommodation elements, for example a rigid foam bed with suitable accommodation spaces for accommodation. of the specimen collection kit working means, in particular the collection and processing tools. Advantageously, this allows tools to be transported in the fixed load state, improves user safety, and facilitates recognition of the possible absence of a tool. In a particularly preferred manner, sliding receptacle spaces are provided whose shape is adapted to the shape of the working means.

If the container equipment is equipped with several container modules, the loading interface is preferably provided in the documentation module, since this contains auxiliary electrical devices. As an alternative, in particular in the case of an electrical connection of the container modules and / or the use of electrically operated tools and / or the accommodation of auxiliary devices in the other modules, the charging interface can be provided in another module container.

Furthermore, the container kit may advantageously be equipped with an emergency module containing medical aids for the user of the sample collection kit. The emergency module (first aid box) is preferably configured to mate with the other container modules, in particular for integration into the stack of the container modules.

The working means and sensors in the sample collection kit can be advantageously selected depending on the specific task, in particular in the collection and processing of algae. The components are preferably equipped with at least one of the following features. The sensors preferably comprise at least one temperature sensor, at least one humidity sensor, at least one UV sensor and / or at least one salinity sensor.

With the temperature sensor, the temperature can be measured during the collection of the plant samples, during transport and, optionally, also during processing. The data logger stores the measured temperature values as sample data.

The humidity sensor is preferably provided for the detection of humidity in the environment of the plant samples collected in the sample container. Each sample container is preferably equipped with a humidity sensor. The humidity data recorded with the humidity sensor is also stored as sample data during the collection of the plant samples and their transport with the data recording equipment. The humidity sensor advantageously makes it possible to detect damage to the sample container during transport. If the sample container is damaged, the plant samples dry out, which can be detected in the recorded humidity data.

The UV sensor is a radiation sensor with which natural UV radiation is recorded in the collection of plant samples and their transport. Preferably, the UV sensor is also coupled to the sample container to capture UV exposure from collected plant samples even during transport.

The salinity sensor is configured to detect the salinity of the sea at the collection point and / or the collected plant samples. The salinity data stored during collection and transport allow characterizing plant samples and recognizing possible intrusion of foreign substances.

The collection tools of the sample collection kit preferably comprise at least one collection net (for example a net to collect algae), at least one collection knife, a feeler gauge, for example a caliper, to record the thicknesses of collected plants (eg, seaweed stalks) and sample bottles to take reference samples, eg, environmental samples, such as seawater from the environment of collected algae.

The processing tools comprise peeling knives, a cutting board and protective clothing for the user of the sample collection kit, particularly during processing in the processing laboratory, such as a hood, gloves, a mask and a protective coat, and / or cleaning devices, such as a cleaning brush. Furthermore, a suction filter may be provided such as a processing tool for housing dry plant components after a drying process.

The auxiliary apparatus in the sample collection kit preferably comprise a satellite receiver, a camera, a clock and / or recording means for the written documentation. Conditions of collection of the plant samples, such as for example accumulation of shells in algae, can advantageously be documented with the camera.

According to a further advantageous embodiment of the invention, at least one of the collecting tools and / or the processing tools may be equipped with a usage indicator which is configured to show a usage of a tool. The usage indicator, in particular a water sensor, is arranged on a surface of the respective tool. In the case of a chemically functional indicator of use, a chemical indicator is provided which, for example, reacts on contact with water. When the tool comes into contact with water, the usage indicator changes color so that the user can see if the tool in question has already been used. In an electronically operating indicator of use, an electronic circuit is provided which, for example, in the event of contact, for example with water, undergoes a change of state that can be read electronically.

Tool usage detection can also be used to lock down data logging equipment. The at least one data logger can be designed for protection against logging, which is activated as soon as a last tool has been used in a specific workflow.

Alternatively or additionally, at least one of the collection tools and / or the processing tools can have a chemical sensor, which is intended to detect substances foreign to plants, for example animal proteins, in particular microorganisms, marine animals or excrements, or inorganic compounds or environmental toxic substances in the plant sample. Advantageously, with the chemical sensor, contaminations of the plant sample can be detected, based for example on a color change.

In the sample collection procedure according to the invention, the plant extract obtained after processing the plant sample is preferably packaged in an extract container that is provided with the identification data of the sample collection kit and the data of the sample. The combination of the plant extract and the identification and sample data is maintained advantageously during subsequent use by the consumer, for example by the manufacturer of the implant.

In a particularly preferred manner, one of the container modules of the sample collection kit is used to house the extract container for return transport from the processing laboratory to the consumer. With the processing module, the data recording equipment can also be sent to the consumer together with the extract container.

Other details and advantages of the invention are described below with reference to the accompanying drawings. They show:

Figure 1: a schematic perspective view of an embodiment of the sample collection kit according to the invention with a stack of several container modules;

Figure 2: a schematic illustration of the contents of a collection module of the sample collection kit;

Figure 3: a schematic illustration of the content of a documentation module of the sample collection kit; Y

Figure 4: A schematic illustration of the contents of the sample collection kit processing module.

The characteristics of the preferred embodiments of the invention are described below by way of example with reference to a sample collection kit for the collection of algae samples for the production of alginate. The algae samples are preferably obtained from macroalgae, in particular from the Laminaria algae species. However, the implementation of the invention is not limited to this example, but is possible correspondingly with other plant samples. The working means for collecting the algae samples that are contained in the sample collection kit are mentioned by way of example. The implementation of the invention is not limited to the use of the specifically mentioned auxiliary tools and apparatus, but can be implemented with modified working means, adapted to the specific application. In particular, the container modules shown in Figures 2 to 4 can be alternatively or additionally equipped with parts of the list of working means mentioned below. The details of the collection and processing of algae samples and the selection of suitable algae species are not explained below, as they are known per se and are common.

The shown embodiment of the sample collection kit 100 according to the invention can be modified and / or supplemented to improve the safety of correct use. In particular, the sample collection kit 100 can be equipped with equipment for access control, for example a lock that can be released wirelessly, in particular by means of a transponder or RFID chip, or actuated with a key (combination lock or mechanical key), a monitoring device to detect the integrity of the power supply, for example with space sensors in individual housing elements in the container modules, and / or a display device to show the operating status, for example status Loading, from Sample Collection Kit 100.

Figure 1 shows a schematic perspective view of a preferred embodiment of the sample collection kit 100 according to the invention (algae kit), whose container equipment 70 comprises a stack of several container modules 71, 72, 73 and an emergency module 78. The container modules 71, 72, 73 are coupled in the closed state through connection elements 77 complementary to each other, which are arranged on the outer sides of the container modules 71, 72, 73. The container modules comprise a collection module 71 (see figure 2), a documentation module 72 (see figure 3) and a processing module 73 (see figure 4). Container modules 71, 72, 73 are graphically and / or textually marked according to their function and equipment (eg 71: "MAR", 72: "DOC", 73: "LAB", 78: "SOS" ). Each of the container modules 71, 72, 73 is a box (suitcase), for example made of plastic and / or metal, with a bottom part 71A and a lid part 71B, which are joined by hinges. In the closed state, the container modules 71, 72, 73 are closed on all sides, so that their interior space can be separated from the environment by materials and provided in a sterile state until the first opening. The transport handles 75 on the front sides and / or the lid parts 71B are designed individually or in combination for manual transport of the container modules 71, 72, 73.

The identification marks 80 on the outer sides of the container modules 71, 72, 73 comprise waterproof markings, for example barcodes, QR codes, readable wireless memory, in particular transponders, and / or character sequence of numbers and / or or letters, which contain identification data of the sample collection kit 100. The working means assembled in the sample collection kit 100 and can be extracted from the container modules are provided with identification marks 80 of the same type or of a Modified type containing the same identification data as the Sample Collection Kit 100.

The documentation module 72 is equipped on its outer surface of the corresponding bottom part with a charging interface 74 in the form of a charging socket (for example, a cooling device socket socket), through which devices electrical operating aids (eg, satellite receivers, mobile phone) in the documentation module 72 through an internal charging apparatus can be supplied with electrical charging current. This ensures that all devices can be prepared for use during collection without damaging the seal of the sample collection kit 100. In addition, a data interface 79 is provided in the documentation module 72, through which data can be read. from an internal data recording equipment.

Figure 2 shows the collection module 71 of the sample collection kit 100 schematically in the open state with a top view of the bottom portion 71A and the lid portion 71B. In the bottom part 71A there is provided a holding platform 76 with accommodation spaces 76A for the collection tools 41, 42, ..., which are used for the collection of the algae samples, and for the sample containers 10 The clamping platform 76 is a form-fit rigid foam bed in which the housing spaces 76A are formed as depressions with the outer shape of the individual components. Furthermore, the collection module 71 contains a schematically shown release kit 71C, which is coupled to a satellite receiver and blocks a closure of the collection module 71 until the collection module 71 is at a predetermined geographic sample collection location. .

The collection tools in the bottom portion 71A comprise two collection knives 42, a feeler gauge 43, with marking elements 44, a rinse or spray bottle 45, two sample bottles 46, and sheet material 47. The knives Harvesting Knife 42 are shown by way of example with usage indicators 42A which, for example, exhibit a color change when Harvesting Knife 42 comes into contact with water. In addition, the harvesting knives 42 can be equipped with chemical sensors to detect foreign substances. The thickness gauge 43 is a ruler or a caliper used to measure the thickness of the collected algae samples. The measured thicknesses are taken with the recording means for the written documentation in the documentation module 72. The marking elements 44 are tapes each bearing an identification mark with the identification data of the sample collection kit 100 and are provided for attachment to the sample containers 10. The marking elements 44 may advantageously be provided at the same time to close the sample container 10. The rinsing bottle 45 contains unsalted or saline water to rinse the algae samples collected prior to accommodation in one of the sample containers 10. With the sample bottles 46 samples from the environment, for example water samples, can be accommodated at the collection site and transported to the processing laboratory together with the harvested algae samples . The sheet material 47 is intended as an auxiliary material, for example for packaging purposes. The collection tools in the cover portion 71B comprise two collection nets 41 for collecting the algae samples from the seawater.

The documentation module 72 which is schematically shown in the open state in figure 3 has a holding platform 76 in the bottom part 72A with accommodation spaces 76A for the auxiliary devices 61, 62, ..., in particular for the purposes of documentation, and in the lid portion 72B a compartment 72C for recording media 64 for written documentation, for example a notepad.

The auxiliary apparatuses in the bottom part 71A comprise at least one satellite receiver 61, a camera 62, a watch 63, a cell phone 65, a printed work instruction manual 66, a writing instrument 67, a charger 68 and clothing protection 69 for the user of the sample collection kit 100. The charger 68 is connected, on the one hand, to the charging interface 74 and, on the other hand, it can be coupled with the auxiliary devices of electrical operation with electrical accumulators through of charging cables (not shown). Protective clothing 69 comprises, for example, a hood or hairnet, mask and gloves for the user in the processing of algae samples and in the preparation of alginate granules.

Furthermore, a sensor arrangement with several sensors 21, 22, ... shown schematically and a data recording equipment 30 (data logger) for recording sample data are arranged in the bottom portion 71A. Data logging equipment 30 is connected to data interface 79. Additional data loggers (not shown) are provided for connection with sensors and for insertion into sample containers 10 with algae samples after harvesting during harvesting. transportation to the processing laboratory.

The sensors 21, 22, ... comprise for example humidity sensors 21 for measuring the humidity in the sample containers 10 after harvesting and during transport, a temperature sensor 22 for measuring the air temperature and / or water during use of the sample collection kit 100, UV sensors 23 for the measurement of UV radiation in the sample containers 10 after collection and during transport and / or a salinity sensor 24.

In modified embodiments of the invention, other additional ancillary apparatus may be contained in the documentation module 72 or other container modules. For example, the data recording equipment 30 can be replaced by a computer, and / or the components 61, 62, 63 and 65 and the data recording equipment 30 can be integrated into a common appliance, such as a microcomputer or a smartphone. . Alternatively or additionally, additional sensors may be provided to record meteorological data, the chlorophyll concentration of the algal samples or morphological data of the algal samples. Furthermore, several satellite receivers 61 may be provided, which are arranged in sample containers 10 during the transportation of the algae samples.

According to figure 4, the processing module 73 comprises in the bottom part 73A two clamping platforms 76.1, 76.2 with accommodation spaces 76A for the processing tools 51, 52, ... and optionally other auxiliary devices and in the cover portion 73B a compartment 73C for other recording means 64 for written documentation or written work instruction manual. Clamping platforms 76.1, 76.2 form two partially overlapping planes with different processing tools 51, 52,

The processing tools comprise, for example, two peeling knives and / or chopping boards 51, a cutting board 52, filter material 53, a storage container 54 for treatment liquids, in particular disinfectants, a cleaning element 55, in particular a brush, and extract containers 56, 57, such as, for example cans 56 and bag 57. The peeling knives and / or chopping boards 51 are shown by way of example with chemical sensors 51A for the detection of foreign substances that present by example a color change in the presence of animal protein in plant samples. Furthermore, the peeling and / or chopping knives 51 can be equipped with usage indicators.

In a specific embodiment, the sample collection kit 100 has a content in accordance with the following list of working means:

- 1 printed work instruction manual 66,

- 6 labels with 80 identification marks,

- 5 address labels to send to the consumer,

- 8 UV resistant plastic bags as 10 sample containers,

- 9 marking elements 44, in each case with a barcode containing the identification data,

- 1 thermometer (min - max),

- 19 pairs of disposable gloves,

- 1 sprayer 45 (volume 11),

- 1 g of disinfectant, for example Micropur,

- 1 garbage bag,

- 3 lockable test sample tubes 46 (50 ml),

- 1 collection net 41 (with carabiner),

- 1 harvesting knife 42,

- 1 chopping board (edge length 15 cm),

- 1 reference model for sizing,

- 1 satellite receiver 61,

- 1 mobile phone,

- 2 data loggers,

- 1 digital camera,

- 1 clock with alarm / wake-up function,

- 1 clipboard with A5 format paper clips as writing support,

- 1 writing device 67,

- 4 lab coats of different sizes,

- 8 hair nets,

- 8 face masks,

- 1 hand brush 55,

- 1 cutting board 52 (30 cm x 22 cm),

- 2 peeling knives 51,

- 12 extract bags of 57 of different sizes,

- 1 filter 53, and

- 1 record book 64.

The collection and processing of seaweed samples to obtain alginate extract comprises, for example, the following steps.

Before the collection phase on the coast or in the open sea, the sample collection kit 100 is loaded. During the collection, a continuous time measurement is performed in the process (clock), a continuous disinfection (for example, with Micropur), a continuous handwritten documentation (clipboard, writing apparatus, record book). After preparing solutions with tap water, a start-up of the data loggers, actual harvesting (immersion of the algae, use of harvesting knives and a net), a cutting of the algae and a size measurement ( knife, feeler gauge). This is followed by disinfection of the algae samples (for example, with Micropur) and packaging of the algae samples in sample containers 10 protected from light (bags, closed with marking elements 44 and fitted with data loggers, satellite receivers). Additionally, water samples are taken in sample bottles 46. After transportation to land and to the processing laboratory, the preparation (processing) is carried out in the laboratory. For this purpose, initially the protective clothing 69 contained in the sample collection kit 100 (gloves, coat, mesh, mask) is placed. All data loggers are read from the sample containers. The algae samples are then cleaned using the hand brush 55 and a micro-cutting knife. Next, the algae are pruned and peeled, a water sample is taken with tap water and the algae are finely cut to form algae granules which, if necessary, can be subjected to a precipitation step to obtain the alginate.

The algae or alginate granulate is dried in a drying cabinet of the processing laboratory and is removed from the drying cabinet with a suction equipment and filter 53. The dried algae granulate is packed in extract containers 56, 57 to empty. The extract containers 56, 57 are provided with an unambiguous code from the sample collection kit 100 (for example, a label with a barcode) and are sent to the consumer together with a thermometer (min-max).

Claims (14)

1. Sample collection kit (100), which is configured for the collection of plant samples, in particular algae samples for biomedical applications, comprising: - at least one sample container (10) which is configured to house plant samples, - a sensor arrangement with several sensors (21, 22, ...) with which sample data can be taken, - a data recording equipment (30) which is configured to record the sample data, - a set of tools with collection tools (41, 42, ...) which are configured for a collection of the samples plants and with processing tools (51, 52, ...) which are configured for the processing of plant samples, - a set of devices with auxiliary devices (61, 62, ...) which can be used in the collection, transport and processing of plant samples and which contain auxiliary devices with electrical operation, and - a container set (70) that is configured to house the set of tools and the set of devices in a state isolated from the environment, characterized by - Identification marks (80) which contain identification data and which are applied to at least one sample container, the collection tools (41, 42, ...) and the processing tools (51, 52, ...), where - the container equipment (70) is equipped with a charging interface (74), through which the auxiliary devices for electrical operation of the set of devices can be supplied with electric charging current. 2. Sample collection kit according to claim 1, comprising - some consumable materials (91, 92, ...) that can be used for the collection and processing of plant samples. Sample collection kit according to one of the preceding claims, in which - the container equipment (70) for maintaining a sterile state of the collection tools (41, 42, ...) and the collection tools processing (51, 52, ...) are configured before the start of the collection. Sample collection kit according to one of the preceding claims, in which - the container equipment has several container modules (71, 72, 73) comprising a collection module (71) containing the collection tools collection (41, 42, ...), a documentation module (72) containing the auxiliary apparatus (61, 62.), and a processing module (73) containing the processing tools (51, 52,. ..). Sample collection kit according to claim 4, in which - the loading interface (74) comprises a loading bushing that is arranged on an outer side of the container equipment (70). Sample collection kit according to one of claims 4 to 5, in which the container modules (71, 72, 73) have at least one of the characteristics: - the collection tools (41, 42, ...) and the processing tools (51, 52, ...) are arranged in each case in the collection module (71) and in the processing module (73) side by side in rows corresponding to a predetermined order of use of the collection tools (41, 42, ...) and the processing tools (51, 52, ...) in the collection and processing of plant samples, - the collection module (71) is equipped with a release equipment (71C) that allows the collection module (71) to be opened exclusively at a predetermined geographic sample collection location, - the container modules (71, 72, 73) are detachably connected to each other, - the container modules (71, 72, 73) have transport handles (75) for manual transport of the container modules (71, 72, 73), - the container modules (71, 72, 73) have the same shape and size, - the container modules (71, 72, 73) in each case have at least one holding platform (76, 76.1, 76.2) with housing elements (76A) for housing the parts of the sample collection kit, Y - the container modules (71, 72, 73) have connecting elements (77) adapted to each other on their outer sides. Sample collection kit according to one of claims 4 to 6, in which - the container equipment (70) has an emergency module (78) that contains medical aids for use by the users of the sample collection kit. Sample collection kit according to one of the preceding claims, having at least one of the characteristics - the sensors (21, 22, ...) comprise at least one temperature sensor, at least one humidity sensor, at least one UV sensor and / or at least one salinity sensor, - the collection tools (41, 42, ...) comprise at least one collection net (41), at least one collection knife (42), a thickness gauge (43), marking elements (44) , a rinse bottle (45) and / or at least one sample bottle (46), - the processing tools (51, 52, ...) comprise at least one peeling knife (51), at least one chopping board, a cutting board (52), filter material (53), a storage container (54 ), a cleaning device (55) and / or transport containers (56, 57), and - the auxiliary devices comprise a satellite receiver (61), a camera (62), a watch (63), a mobile phone (65), a printed work instruction manual (66), a writing device (67), a charger (68), a recording means (64) for written documentation and / or protective clothing (69) for the user of the sample collection kit. Sample collection kit according to one of the preceding claims, in which - at least one of the collection tools (41, 42, ...) and / or the processing tools (51, 52, ...) presents a usage indicator (42A) which is configured to show a tool usage, and / or - at least one of the collection tools (41, 42, ...) and / or the processing tools (51, 52, ...) has a chemical sensor (51A) that is configured for the detection of foreign substances in the vegetable sample. 10. Sample collection procedure for the collection of plant samples, in particular algae samples for biomedical applications, in which a sample collection kit according to one of the preceding claims is used, with the following steps: (a) collection of plant samples in a natural sample collection place, in which the collection tools (41, 42, ...) are used, the collected plant samples are housed in at least one sample container ( 10) and the data of the samples are taken in the data recording equipment (30), (b) transportation of the at least one sample container (10) and the sample collection kit (100) to a processing laboratory, where the data of the samples are complemented in the data recording equipment (30), (c) production of a plant extract in the processing laboratory, where the processing tools (51, 52, ...) are used and the sample data are additionally complemented in the data recording equipment (30), Y (d) provision of the plant extract and a data set, comprising the sample data and the identification data, for a subsequent use of the plant extract. 11. Sample collection method according to claim 10, in which - the sample data includes information on the place of collection, the temperature during collection, transport and processing, and the transport route. 12. Sample collection method according to one of claims 10 to 11, in which - in step (a), environmental samples, in particular water samples, are additionally taken from the place where the samples were collected. Sample collection method according to one of claims 10 to 12, in which - in step (d) the plant extract is packaged in an extract container (56, 57) that is provided with the identification data of the sample collection kit and the sample data, and / or - in step (d), the extract container (56, 57) with the plant extract is arranged in the container equipment (70) and is provided for subsequent transport to a consumer. Sample collection method according to one of claims 10 to 13, in which - the plant samples include algae.