ES2114504B1 - METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. - Google Patents
METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT.Info
- Publication number
- ES2114504B1 ES2114504B1 ES9601945A ES9601945A ES2114504B1 ES 2114504 B1 ES2114504 B1 ES 2114504B1 ES 9601945 A ES9601945 A ES 9601945A ES 9601945 A ES9601945 A ES 9601945A ES 2114504 B1 ES2114504 B1 ES 2114504B1
- Authority
- ES
- Spain
- Prior art keywords
- solid support
- deoxyoligonucleotides
- enzymatic synthesis
- synthesis
- transferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA LA SINTESIS ENZIMATICA DE DESOXIOLIGONUCLEOTIDOS EN SOPORTE SOLIDO, QUE CONSISTE EN REALIZAR LA SINTESIS SOBRE UN SOPORTE SOLIDO EN LA DIRECCION (5'') A (3''), UTILIZANDO LA TRANSFERASA TERMINAL, UNA ADN POLIMERASA INDEPENDIENTE DE MOLDE QUE PREFERENTEMENTE, CATALIZA LA INCORPORACION DE DESOXIRRIBONUCLEOSIDOS 5" TRIFOSFATOS A TERMINALES 3", HIDROXILO DE ADN MONOCATENARIO Y SE EMPLEA DESOXIRRIBONUCLEOTIDOS CON SU HIDROXILO (3'') TRANSITORIAMENTE PROTEGIDO CON OBJETO DE AÑADIR UN SOLO NUCLEOTIDO EN CADA CICLO.METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT, WHICH CONSISTS OF PERFORMING THE SYNTHESIS ON A SOLID SUPPORT IN THE DIRECTION (5 '') TO (3 ''), USING THE TERMINAL TRANSFERASE, INDEPENDENT MANDATORY TRANSFERASE, CATALYSIS THE INCORPORATION OF 5 "TRIPHOSPHATES TO 3" TERMINALS, HYDROXYL OF MONOCATENARY DNA AND DEOXYRIBONUCLEOTIDES ARE USED WITH ITS TRANSITIONALLY PROTECTED NUCLEAR CANDIDATE WITH A 3-YEAR OBJECT.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2114504A1 ES2114504A1 (en) | 1998-05-16 |
ES2114504B1 true ES2114504B1 (en) | 1999-02-01 |
Family
ID=8296028
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9601945A Expired - Fee Related ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2114504B1 (en) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3301833A1 (en) * | 1983-01-20 | 1984-07-26 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | METHOD FOR SIMULTANEOUS SYNTHESIS OF SEVERAL OLIGONOCLEOTIDES IN A SOLID PHASE |
US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
US4816571A (en) * | 1987-06-04 | 1989-03-28 | Applied Biosystems, Inc. | Chemical capping by phosphitylation during oligonucleotide synthesis |
SE9003743D0 (en) * | 1990-11-26 | 1990-11-26 | Pharmacia Ab | METHOD AND MEANS FOR OLIGONUCLEOTIDE SYNTHESIS |
US5872244A (en) * | 1994-09-02 | 1999-02-16 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
-
1996
- 1996-09-13 ES ES9601945A patent/ES2114504B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2114504A1 (en) | 1998-05-16 |
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Legal Events
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---|---|---|---|
EC2A | Search report published |
Date of ref document: 19980516 Kind code of ref document: A1 Effective date: 19980516 |
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Effective date: 20161227 |