ES2078174A1 - Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19 - Google Patents
Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19Info
- Publication number
- ES2078174A1 ES2078174A1 ES09302728A ES9302728A ES2078174A1 ES 2078174 A1 ES2078174 A1 ES 2078174A1 ES 09302728 A ES09302728 A ES 09302728A ES 9302728 A ES9302728 A ES 9302728A ES 2078174 A1 ES2078174 A1 ES 2078174A1
- Authority
- ES
- Spain
- Prior art keywords
- methods
- molecular diagnostic
- diagnostic methods
- differentiation
- brucella
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19. A series of molecular diagnostic methods which can be used to differentiate between the Brucella abortus B19 vaccine lineage and all other lineages of the genus Brucella are described. In one case, amplification of the genome is applied using oligonucleotides derived from the region containing the genes coding for the erythritol utilization route as indicators. Another group of methods is based on the hybridization of genome DNA using test oligonucleotides from the eri region mentioned. In this case the hybridization technique known as the Southern type or alternatively the "dot blot" procedure may be used. Finally these methods are applied to the identification of micro-organisms of the genus Brucella in any biological sample. The new procedures have advantages over conventional immunological and culture methods providing greater specificity and speed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09302728A ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09302728A ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2078174A1 true ES2078174A1 (en) | 1995-12-01 |
ES2078174B1 ES2078174B1 (en) | 1996-08-16 |
Family
ID=8284078
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09302728A Expired - Fee Related ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2078174B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006097347A2 (en) * | 2005-03-17 | 2006-09-21 | Instituto Científico Y Tecnológico De Navarra, S.A. | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
-
1993
- 1993-12-16 ES ES09302728A patent/ES2078174B1/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
US4358535B1 (en) * | 1980-12-08 | 1986-05-13 | ||
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683202B1 (en) * | 1985-03-28 | 1990-11-27 | Cetus Corp |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006097347A2 (en) * | 2005-03-17 | 2006-09-21 | Instituto Científico Y Tecnológico De Navarra, S.A. | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
WO2006097347A3 (en) * | 2005-03-17 | 2007-01-04 | Inst Cientifico Tecnol Navarra | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Also Published As
Publication number | Publication date |
---|---|
ES2078174B1 (en) | 1996-08-16 |
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Legal Events
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