ES2048646A1 - Prodn. of VP2 protein of African horse plague virus - by synthesising the DNA molecule codifying for the protein and expression in suitable host systems - Google Patents

Prodn. of VP2 protein of African horse plague virus - by synthesising the DNA molecule codifying for the protein and expression in suitable host systems

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Publication number
ES2048646A1
ES2048646A1 ES9200822A ES9200822A ES2048646A1 ES 2048646 A1 ES2048646 A1 ES 2048646A1 ES 9200822 A ES9200822 A ES 9200822A ES 9200822 A ES9200822 A ES 9200822A ES 2048646 A1 ES2048646 A1 ES 2048646A1
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Spain
Prior art keywords
protein
synthesising
expression
obtaining
cdna molecule
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9200822A
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Spanish (es)
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ES2048646B1 (en
Inventor
Olmo Vela
Alvarez Casal
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Inmunologia y Genetica Aplicada SA
Original Assignee
Ercros SA
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Priority to ES9200822A priority Critical patent/ES2048646B1/en
Publication of ES2048646A1 publication Critical patent/ES2048646A1/en
Application granted granted Critical
Publication of ES2048646B1 publication Critical patent/ES2048646B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procedure for obtaining the VP2 protein from the African horse sickness virus (AHSV).The procedure comprises an initial phase of obtaining a cDNA molecule which codifies the complete sequence of the VP2 protein using the pattern of specific oligonucleotides for the end regions of the VP2 gene and then synthesising the full length of the cDNA molecule. In a second phase, the recombinant VP2 proteins are obtained through the expression of said cDNA molecule in suitable systems such as baculovirus multiplied in insect cells.The recombinant VP2 proteins obtained can be used in the formulation of vaccines to protect horses from the infection caused by AHSV and can also be used to detect the presence of said virus in animals suspected of being infected with AHSV.The process consists of (a) obtaining a molecule of cDNA which codifies for the complete sequence of protein VP2, by designing specific oligo-nucleotides for the terminal regions of the VP2 gene and subsequently synthesising the cDNA molecule of complete length and (b) obtaining recombinant VP2 proteins by expression of the cDNA molecule in suitable systems, e.g. baculovirus multiplied in insect cells.
ES9200822A 1992-04-15 1992-04-15 PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV) Expired - Fee Related ES2048646B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9200822A ES2048646B1 (en) 1992-04-15 1992-04-15 PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9200822A ES2048646B1 (en) 1992-04-15 1992-04-15 PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV)

Publications (2)

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ES2048646A1 true ES2048646A1 (en) 1994-03-16
ES2048646B1 ES2048646B1 (en) 1994-10-01

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ES9200822A Expired - Fee Related ES2048646B1 (en) 1992-04-15 1992-04-15 PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0127839A2 (en) * 1983-05-27 1984-12-12 THE TEXAS A&M UNIVERSITY SYSTEM Method for producing a recombinant baculovirus expression vector
WO1990001556A1 (en) * 1988-08-05 1990-02-22 Mount Sinai School Of Medicine Of The City University Of New York In vivo infection of live insects with a recombinant baculovirus
GB2228486A (en) * 1989-02-27 1990-08-29 Univ Ottawa Improved baculovirus expression system capable of producing foreign gene proteins at high levels
WO1991004330A1 (en) * 1989-09-14 1991-04-04 Rijksuniversiteit Te Leiden Human parvovirus b19 proteins and virus-like particles, their production and their use in diagnostic assays and vaccines

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0127839A2 (en) * 1983-05-27 1984-12-12 THE TEXAS A&M UNIVERSITY SYSTEM Method for producing a recombinant baculovirus expression vector
WO1990001556A1 (en) * 1988-08-05 1990-02-22 Mount Sinai School Of Medicine Of The City University Of New York In vivo infection of live insects with a recombinant baculovirus
GB2228486A (en) * 1989-02-27 1990-08-29 Univ Ottawa Improved baculovirus expression system capable of producing foreign gene proteins at high levels
WO1991004330A1 (en) * 1989-09-14 1991-04-04 Rijksuniversiteit Te Leiden Human parvovirus b19 proteins and virus-like particles, their production and their use in diagnostic assays and vaccines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KAWASAKI, S.E. et al. "Detection of gene expression", pp. 89-97. ERLICH H.A. (ed) "PCR Technology: principles and applications for DNA amplification". Octubre 1989, STOCKTON PRESS, NEW YORK, USA. * Todo el documento * *
MARTINEZ TORRE CUADRADA, J.L. et al. "Molecular cloning and nucleotide sequence of African horsesickness virus serotype 4 L2 gene encoding VP2, a serotype specific capsid protein involved in virus neutralization". 6-Abril-1992. GenBank Database. USA. * Secuencia * *

Also Published As

Publication number Publication date
ES2048646B1 (en) 1994-10-01

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PC2A Transfer of patent

Owner name: INMUNOLOGIA Y GENETICA APLICADA, S.A.

FD2A Announcement of lapse in spain

Effective date: 20180806