ES2048646A1 - Prodn. of VP2 protein of African horse plague virus - by synthesising the DNA molecule codifying for the protein and expression in suitable host systems - Google Patents
Prodn. of VP2 protein of African horse plague virus - by synthesising the DNA molecule codifying for the protein and expression in suitable host systemsInfo
- Publication number
- ES2048646A1 ES2048646A1 ES9200822A ES9200822A ES2048646A1 ES 2048646 A1 ES2048646 A1 ES 2048646A1 ES 9200822 A ES9200822 A ES 9200822A ES 9200822 A ES9200822 A ES 9200822A ES 2048646 A1 ES2048646 A1 ES 2048646A1
- Authority
- ES
- Spain
- Prior art keywords
- protein
- synthesising
- expression
- obtaining
- cdna molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Procedure for obtaining the VP2 protein from the African horse sickness virus (AHSV).The procedure comprises an initial phase of obtaining a cDNA molecule which codifies the complete sequence of the VP2 protein using the pattern of specific oligonucleotides for the end regions of the VP2 gene and then synthesising the full length of the cDNA molecule. In a second phase, the recombinant VP2 proteins are obtained through the expression of said cDNA molecule in suitable systems such as baculovirus multiplied in insect cells.The recombinant VP2 proteins obtained can be used in the formulation of vaccines to protect horses from the infection caused by AHSV and can also be used to detect the presence of said virus in animals suspected of being infected with AHSV.The process consists of (a) obtaining a molecule of cDNA which codifies for the complete sequence of protein VP2, by designing specific oligo-nucleotides for the terminal regions of the VP2 gene and subsequently synthesising the cDNA molecule of complete length and (b) obtaining recombinant VP2 proteins by expression of the cDNA molecule in suitable systems, e.g. baculovirus multiplied in insect cells.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9200822A ES2048646B1 (en) | 1992-04-15 | 1992-04-15 | PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9200822A ES2048646B1 (en) | 1992-04-15 | 1992-04-15 | PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV) |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2048646A1 true ES2048646A1 (en) | 1994-03-16 |
ES2048646B1 ES2048646B1 (en) | 1994-10-01 |
Family
ID=8276762
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9200822A Expired - Fee Related ES2048646B1 (en) | 1992-04-15 | 1992-04-15 | PROCEDURE FOR THE OBTAINING OF AFRICAN EQUINE PLASTER VIRUS PROTEIN VP2 (AHSV) |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2048646B1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0127839A2 (en) * | 1983-05-27 | 1984-12-12 | THE TEXAS A&M UNIVERSITY SYSTEM | Method for producing a recombinant baculovirus expression vector |
WO1990001556A1 (en) * | 1988-08-05 | 1990-02-22 | Mount Sinai School Of Medicine Of The City University Of New York | In vivo infection of live insects with a recombinant baculovirus |
GB2228486A (en) * | 1989-02-27 | 1990-08-29 | Univ Ottawa | Improved baculovirus expression system capable of producing foreign gene proteins at high levels |
WO1991004330A1 (en) * | 1989-09-14 | 1991-04-04 | Rijksuniversiteit Te Leiden | Human parvovirus b19 proteins and virus-like particles, their production and their use in diagnostic assays and vaccines |
-
1992
- 1992-04-15 ES ES9200822A patent/ES2048646B1/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0127839A2 (en) * | 1983-05-27 | 1984-12-12 | THE TEXAS A&M UNIVERSITY SYSTEM | Method for producing a recombinant baculovirus expression vector |
WO1990001556A1 (en) * | 1988-08-05 | 1990-02-22 | Mount Sinai School Of Medicine Of The City University Of New York | In vivo infection of live insects with a recombinant baculovirus |
GB2228486A (en) * | 1989-02-27 | 1990-08-29 | Univ Ottawa | Improved baculovirus expression system capable of producing foreign gene proteins at high levels |
WO1991004330A1 (en) * | 1989-09-14 | 1991-04-04 | Rijksuniversiteit Te Leiden | Human parvovirus b19 proteins and virus-like particles, their production and their use in diagnostic assays and vaccines |
Non-Patent Citations (2)
Title |
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KAWASAKI, S.E. et al. "Detection of gene expression", pp. 89-97. ERLICH H.A. (ed) "PCR Technology: principles and applications for DNA amplification". Octubre 1989, STOCKTON PRESS, NEW YORK, USA. * Todo el documento * * |
MARTINEZ TORRE CUADRADA, J.L. et al. "Molecular cloning and nucleotide sequence of African horsesickness virus serotype 4 L2 gene encoding VP2, a serotype specific capsid protein involved in virus neutralization". 6-Abril-1992. GenBank Database. USA. * Secuencia * * |
Also Published As
Publication number | Publication date |
---|---|
ES2048646B1 (en) | 1994-10-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC2A | Transfer of patent |
Owner name: INMUNOLOGIA Y GENETICA APLICADA, S.A. |
|
FD2A | Announcement of lapse in spain |
Effective date: 20180806 |