ES2012737A6 - Method for producing thermally stable alpha-amylase. - Google Patents

Method for producing thermally stable alpha-amylase.

Info

Publication number
ES2012737A6
ES2012737A6 ES8902148A ES8902148A ES2012737A6 ES 2012737 A6 ES2012737 A6 ES 2012737A6 ES 8902148 A ES8902148 A ES 8902148A ES 8902148 A ES8902148 A ES 8902148A ES 2012737 A6 ES2012737 A6 ES 2012737A6
Authority
ES
Spain
Prior art keywords
amylase
thermally stable
carbon source
medium
during cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8902148A
Other languages
Spanish (es)
Inventor
James C Chen
William R Lamm
Francis M Racine
Hal R Turner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Great Lakes Chemical Corp
Original Assignee
Great Lakes Chemical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Great Lakes Chemical Corp filed Critical Great Lakes Chemical Corp
Publication of ES2012737A6 publication Critical patent/ES2012737A6/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

Abstract

A process for the production of thermally stable alpha-amylase comprising the steps of: cultivating B. licheniformis ATCC 53,722 in a nutrient medium which contains as a primary carbon source a carbohydrate which normally represses alpha-amylase synthesis, selected from the group consisting of dextrose, starch hydrolysate, glycerol, xylose, mannose, sucrose and mixtures thereof feeding said primary carbon source to said medium during cultivation maintaining the level of carbon dioxide evolution from the medium during cultivation between about 4.5 % and 6.5 % of the off-gas when the air flow is 0.5 to 1.0 vvm by controlling the rate at which the primary carbon source is fed to said medium during cultivation and maintaining the pH of the medium between 6.5 and 7.0.
ES8902148A 1988-06-21 1989-01-20 Method for producing thermally stable alpha-amylase. Expired - Fee Related ES2012737A6 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US20942688A 1988-06-21 1988-06-21

Publications (1)

Publication Number Publication Date
ES2012737A6 true ES2012737A6 (en) 1990-04-01

Family

ID=22778710

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8902148A Expired - Fee Related ES2012737A6 (en) 1988-06-21 1989-01-20 Method for producing thermally stable alpha-amylase.

Country Status (9)

Country Link
EP (1) EP0380617A4 (en)
JP (1) JPH03501328A (en)
KR (1) KR900702012A (en)
BR (1) BR8906981A (en)
DK (1) DK46090A (en)
ES (1) ES2012737A6 (en)
FI (1) FI900838A0 (en)
HU (1) HUT53934A (en)
WO (1) WO1989012679A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2276352T3 (en) 2008-04-15 2020-07-13 The United States Of America, As Represented By The Secretary Of Agriculture Protein concentrate from starch containing grains: composition, method of making, and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4473645A (en) * 1983-03-30 1984-09-25 Nabisco Brands, Inc. Process for producing thermally stable alpha-amylase
ES8602115A1 (en) * 1983-10-11 1985-11-01 Espanola Petrol A process for producing thermostable alpha-amylases by culturing micro-organisms at elevated temperatures.

Also Published As

Publication number Publication date
EP0380617A4 (en) 1991-09-25
DK46090D0 (en) 1990-02-21
EP0380617A1 (en) 1990-08-08
HUT53934A (en) 1990-12-28
HU894144D0 (en) 1990-09-28
FI900838A0 (en) 1990-02-20
BR8906981A (en) 1990-12-18
DK46090A (en) 1990-02-21
KR900702012A (en) 1990-12-05
WO1989012679A1 (en) 1989-12-28
JPH03501328A (en) 1991-03-28

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Legal Events

Date Code Title Description
SA6 Expiration date (snapshot 920101)

Free format text: 2009-01-20

FD1A Patent lapsed

Effective date: 19991108