EP3048109A4 - Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom - Google Patents

Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom Download PDF

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Publication number
EP3048109A4
EP3048109A4 EP14846521.4A EP14846521A EP3048109A4 EP 3048109 A4 EP3048109 A4 EP 3048109A4 EP 14846521 A EP14846521 A EP 14846521A EP 3048109 A4 EP3048109 A4 EP 3048109A4
Authority
EP
European Patent Office
Prior art keywords
antibody
purification method
obtained therefrom
novel
antibody obtained
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14846521.4A
Other languages
German (de)
French (fr)
Other versions
EP3048109A1 (en
Inventor
Kazunobu MINAKUCHI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Publication of EP3048109A1 publication Critical patent/EP3048109A1/en
Publication of EP3048109A4 publication Critical patent/EP3048109A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1271—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Micrococcaceae (F), e.g. Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/626—Diabody or triabody

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP14846521.4A 2013-09-17 2014-09-16 Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom Withdrawn EP3048109A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013192378 2013-09-17
JP2013192379 2013-09-17
PCT/JP2014/074452 WO2015041218A1 (en) 2013-09-17 2014-09-16 Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom

Publications (2)

Publication Number Publication Date
EP3048109A1 EP3048109A1 (en) 2016-07-27
EP3048109A4 true EP3048109A4 (en) 2017-04-19

Family

ID=52688867

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14846521.4A Withdrawn EP3048109A4 (en) 2013-09-17 2014-09-16 Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom

Country Status (7)

Country Link
US (1) US10519195B2 (en)
EP (1) EP3048109A4 (en)
JP (2) JPWO2015041218A1 (en)
KR (1) KR20160054597A (en)
CN (1) CN105555795A (en)
SG (1) SG11201602061YA (en)
WO (1) WO2015041218A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2016121701A1 (en) 2015-01-26 2017-11-09 株式会社カネカ Affinity separation matrix for protein purification containing immunoglobulin κ chain variable region
EP3252158A4 (en) 2015-01-26 2018-07-18 Kaneka Corporation Mutant immunoglobulin kappa chain variable region-binding peptide
JP6709024B2 (en) * 2015-07-03 2020-06-10 国立研究開発法人医薬基盤・健康・栄養研究所 Peptide or protein fractionation method
JP7084301B2 (en) * 2015-08-21 2022-06-14 エフ．ホフマン－ラ ロシュ アーゲー Affinity chromatography purification method using low conductivity wash buffer
BR112018008113A2 (en) * 2015-10-23 2018-11-06 Fujifilm Corporation An affinity chromatography carrier and a refining method of biological material
JP6737579B2 (en) * 2015-10-30 2020-08-12 Jsr株式会社 Affinity chromatography carrier, chromatography column, purification method, and target substance purified by the method
WO2017191747A1 (en) * 2016-05-06 2017-11-09 株式会社カネカ Method for producing protein including κ chain variable region
WO2017195638A1 (en) 2016-05-09 2017-11-16 株式会社カネカ Method for refining antibody or antibody fragment containing κ-chain variable region
WO2019039545A1 (en) 2017-08-23 2019-02-28 Jsr株式会社 Chromatography carrier, ligand-immobilizing carrier, chromatography column, target substance purification method, and chromatography carrier production method
JP7367694B2 (en) 2018-11-06 2023-10-24 Jsr株式会社 Method for producing an organic sulfur compound, carrier, method for producing the carrier, ligand-immobilized carrier, chromatography column, and method for detecting or isolating a target substance
US20220367081A1 (en) * 2019-07-02 2022-11-17 Nihon Medi-Physics Co., Ltd. METHOD FOR PURIFYING 226Ra-CONTAINING SOLUTION, METHOD FOR PRODUCING 226Ra TARGET, AND METHOD FOR PRODUCING 225Ac
WO2022202466A1 (en) 2021-03-25 2022-09-29 Jsr株式会社 Method for producing chromatographic carrier, method for producing chromatography column, and chromatographic carrier
EP4316625A1 (en) 2021-03-25 2024-02-07 JSR Corporation Method for producing carrier for chromatographic use, method for producing chromatography column, and carrier for chromatographic use
WO2023058409A1 (en) 2021-10-05 2023-04-13 Jsr株式会社 Method for filling column with chromatography carrier, method for storing slurry, and slurry

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2027921A2 (en) * 2007-08-14 2009-02-25 Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same.
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
WO2010141039A1 (en) * 2008-10-20 2010-12-09 Abbott Laboratories Isolation and purification of antibodies using protein a affinity chromatography
WO2011017514A1 (en) * 2009-08-07 2011-02-10 Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
WO2011050071A2 (en) * 2009-10-20 2011-04-28 Abbott Laboratories Isolation and purification of anti-il-13 antibodies using protein a affinity chromatography

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3932557A (en) 1972-12-19 1976-01-13 Gulf Research & Development Company Reactive hydrophilic epoxy containing polymer
US4145366A (en) * 1974-11-18 1979-03-20 Teijin Limited Process for isomerizing one of the geometric isomers of an α,β-unsaturated aldehyde to its corresponding other geometric isomer
US4210723A (en) 1976-07-23 1980-07-01 The Dow Chemical Company Method of coupling a protein to an epoxylated latex
US4772653A (en) 1985-11-08 1988-09-20 Amoco Corporation Stain resistant cookware from blends comprising an interpolymer of an unsaturated dicarboxylic acid compound
US5260373A (en) 1987-03-13 1993-11-09 Repligen Corporation Immobilized immunoglobulin-binding proteins
SE467308B (en) 1990-10-22 1992-06-29 Berol Nobel Ab SOLID SURFACE COATED WITH A HYDROPHILIC SURFACE WITH COVALENTLY BONDED BIOPOLYMERS, SET TO MAKE SUCH A SURFACE AND CONJUGATED THEREOF
JPH05202098A (en) 1992-01-29 1993-08-10 Snow Brand Milk Prod Co Ltd Production of physiologically active substance from lactic material
JPH06228200A (en) 1993-01-29 1994-08-16 Snow Brand Milk Prod Co Ltd Production of complement c3c
SE9503925D0 (en) 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separation medium for IgG
US5874165A (en) 1996-06-03 1999-02-23 Gore Enterprise Holdings, Inc. Materials and method for the immobilization of bioactive species onto polymeric subtrates
DE69907439T3 (en) * 1998-05-06 2014-01-02 Genentech, Inc. Purification of proteins by ion exchange chromatography
WO2000009654A2 (en) 1998-08-12 2000-02-24 Zycos Inc. Profiling and cataloging expressed protein tags
US20040224338A1 (en) 1998-08-12 2004-11-11 Zycos Inc., A Delaware Corporation Profiling and cataloging expressed protein tags
SE0200943D0 (en) 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US7709209B2 (en) 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
ITMI20020729A1 (en) 2002-04-08 2003-10-08 Resindion S R L SUPPORTS FOR THE COVALENT IMMOBILIZATION OF ENZYMES
ES2214136B1 (en) 2003-02-21 2007-05-16 Consejo Sup. De Invest. Cientificas. NEW IMMOBILIZATION METHOD OF ENZYMES AND OTHER BIO-MACROMOLECULES ON SUPPORTS ACTIVATED WITH EPOXIDE GROUPS CONTAINING IONIZED GROUPS IN THE SPACER ARM THAT JOINS EACH EPOXIDE GROUP TO THE SUPPORT SURFACE.
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
SE0400501D0 (en) 2004-02-27 2004-02-27 Amersham Biosciences Ab Antibody purification
AU2005216847B2 (en) 2004-02-27 2010-04-01 Cytiva Bioprocess R&D Ab A process for the purification of antibodies
SE0400886D0 (en) * 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
US8101425B1 (en) 2004-06-09 2012-01-24 Pathogen Removal And Diagnostic Technologies Inc. Particles embedded in a porous substrate for removing target analyte from a sample
US9266041B2 (en) 2004-10-21 2016-02-23 Ge Healthcare Bio-Sciences Ab Chromatography ligand
US7867784B2 (en) 2004-10-21 2011-01-11 Ge Healthcare Bio-Science Ab Chromatography ligand
NZ562949A (en) 2005-04-11 2009-05-31 Medarex Inc Protein purification using HCIC and ion exchange chromatography
TWI320788B (en) 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
US7833723B2 (en) * 2006-01-06 2010-11-16 Millipore Corporation Affinity chromatography matrices and methods of making and using the same
WO2009017491A1 (en) 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
WO2008049106A2 (en) 2006-10-19 2008-04-24 Centocor Ortho Biotech Inc. Process for preparing unaggregated antibody fc domains
WO2008085988A1 (en) 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
US7691980B2 (en) 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7999085B2 (en) 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
JP5999899B2 (en) 2008-04-08 2016-09-28 バイオ−ラッド ラボラトリーズ インコーポレーティッド Chromatographic purification of antibodies
CN103396480A (en) * 2008-05-15 2013-11-20 诺沃—诺迪斯克有限公司 Antibody purification process
EP2321338A1 (en) 2008-08-14 2011-05-18 Merck Sharp & Dohme Corp. Methods for purifying antibodies using protein a affinity chromatography
JP2010133734A (en) 2008-12-02 2010-06-17 Tosoh Corp Carboxylation carrier for affinity chromatography, and separating agent for affinity chromatography using the same
JP2010133733A (en) 2008-12-02 2010-06-17 Tosoh Corp Cation exchanger, method for manufacturing the same and application thereof
US8497358B2 (en) 2008-12-19 2013-07-30 Takeda Pharmaceutical Company Limited Antibody purification method
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
US8277649B2 (en) 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
JP5952185B2 (en) 2010-03-24 2016-07-13 株式会社カネカ Proteins that specifically bind to immunoglobulins and immunoglobulin-binding affinity ligands
JP2013544524A (en) * 2010-12-06 2013-12-19 ターポン バイオシステムズ，インコーポレイテッド A continuous process for biological products.
JP6335785B2 (en) 2012-09-03 2018-05-30 株式会社カネカ Mixed-mode antibody affinity separation matrix and purification method and target molecule using the same
EP3301115B1 (en) * 2013-05-06 2021-11-10 Sanofi Continuous multistep process for purifying antibodies

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2027921A2 (en) * 2007-08-14 2009-02-25 Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same.
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
WO2010141039A1 (en) * 2008-10-20 2010-12-09 Abbott Laboratories Isolation and purification of antibodies using protein a affinity chromatography
WO2011017514A1 (en) * 2009-08-07 2011-02-10 Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
WO2011050071A2 (en) * 2009-10-20 2011-04-28 Abbott Laboratories Isolation and purification of anti-il-13 antibodies using protein a affinity chromatography

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2015041218A1 *

Also Published As

Publication number Publication date
US10519195B2 (en) 2019-12-31
SG11201602061YA (en) 2016-04-28
US20160237113A1 (en) 2016-08-18
JP2019034963A (en) 2019-03-07
JPWO2015041218A1 (en) 2017-03-02
WO2015041218A1 (en) 2015-03-26
EP3048109A1 (en) 2016-07-27
KR20160054597A (en) 2016-05-16
CN105555795A (en) 2016-05-04

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