EP1092041A4 - Exonuclease-mediated nucleic acid reassembly in directed evolution - Google Patents

Exonuclease-mediated nucleic acid reassembly in directed evolution

Info

Publication number
EP1092041A4
EP1092041A4 EP00919763A EP00919763A EP1092041A4 EP 1092041 A4 EP1092041 A4 EP 1092041A4 EP 00919763 A EP00919763 A EP 00919763A EP 00919763 A EP00919763 A EP 00919763A EP 1092041 A4 EP1092041 A4 EP 1092041A4
Authority
EP
European Patent Office
Prior art keywords
exonuclease
nucleic acid
directed evolution
mediated nucleic
acid reassembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919763A
Other languages
German (de)
French (fr)
Other versions
EP1092041A1 (en
Inventor
Jay M Short
Tsotne David Djavakhishvili
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1092041A1 publication Critical patent/EP1092041A1/en
Publication of EP1092041A4 publication Critical patent/EP1092041A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00919763A 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution Withdrawn EP1092041A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US276860 1999-03-26
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
US332835 1999-06-14
PCT/US2000/008245 WO2000058517A1 (en) 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution

Publications (2)

Publication Number Publication Date
EP1092041A1 EP1092041A1 (en) 2001-04-18
EP1092041A4 true EP1092041A4 (en) 2001-09-19

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919763A Withdrawn EP1092041A4 (en) 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution

Country Status (6)

Country Link
EP (1) EP1092041A4 (en)
JP (1) JP2004500019A (en)
AU (1) AU4039400A (en)
CA (1) CA2329122A1 (en)
MX (1) MXPA00011569A (en)
WO (1) WO2000058517A1 (en)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1073710A4 (en) * 1999-02-04 2007-12-19 Verenium Corp Non-stochastic generation of genetic vaccines and enzymes
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
DE19953854C2 (en) * 1999-11-09 2002-01-17 Max Planck Gesellschaft Process for the production of biopolymers with modified properties
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
CN102690799A (en) 2000-12-01 2012-09-26 维莱尼姆公司 Enzymes having dehalogenase activity and methods of use thereof
AU2002227883A1 (en) 2001-01-25 2002-08-06 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DE60220155T2 (en) 2001-05-18 2008-02-07 Danisco A/S PROCESS FOR PREPARING DOUGH WITH AN ENZYME
EP1840211A1 (en) 2001-10-31 2007-10-03 Danisco A/S Pyranosone dehydratase from phanerochaete chrysosporium
WO2004064987A2 (en) 2003-01-17 2004-08-05 Danisco A/S Method
DK2119771T3 (en) 2003-12-24 2018-12-10 Dupont Nutrition Biosci Aps PROTEINS
AU2005263954B2 (en) 2004-07-16 2011-04-07 Dupont Nutrition Biosciences Aps Enzymatic oil-degumming method
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
DK1844144T3 (en) * 2005-01-10 2010-07-19 Medimmune Ltd Method of Mutagenesis
CN101652474B (en) 2007-01-25 2012-06-27 丹尼斯科有限公司 Production of a lipid acyltransferase from transformed bacillus licheniformis cells
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
PL2139515T5 (en) 2007-03-30 2024-04-08 The Research Foundation Of The State University Of New York Attenuated viruses useful for vaccines
BRPI0821253A2 (en) 2007-12-21 2015-06-16 Danisco Degumming method, method of treatment of a gum phase and use of lipid acyltransferase in water degumming of an edible oil
KR102265757B1 (en) 2009-03-09 2021-06-17 바이오아트라, 인코퍼레이티드 Mirac proteins
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
CN102428177B (en) 2009-05-19 2015-01-14 杜邦营养生物科学有限公司 Method for improving stackablity of bread and products
AR077042A1 (en) 2009-06-12 2011-07-27 Danisco METHOD TO TREAT A COMPOSITION CONTAINING PYROPHIOPHYTIN
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
BR112012022998A2 (en) 2010-03-12 2016-08-02 Dupont Nutrition Biosci Aps process for refining a plant oil, refined plant oil, distillate and use of a process
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
US20130085287A1 (en) 2010-06-17 2013-04-04 Dupont Nutrition Biosciences Aps Process
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
DK2678438T3 (en) 2011-02-23 2017-10-02 Dupont Nutrition Biosci Aps ENZYME TREATMENT OF CHLOROPHYL IN PLANT OILS
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
AR085253A1 (en) 2011-02-23 2013-09-18 Danisco METHOD TO PRODUCE A CHLOROPHYLASE
AU2012241055A1 (en) 2011-04-08 2013-08-15 Danisco Us, Inc. Compositions
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
US10226744B2 (en) 2012-10-19 2019-03-12 Danisco Us Inc Stabilization of biomimetic membranes
EP3083936B1 (en) 2013-12-19 2018-07-04 Danisco US Inc. Use of hydrophobins to increase gas transfer in aerobic fermentation processes
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
WO2016062855A1 (en) 2014-10-24 2016-04-28 Dupont Nutrition Biosciences Aps Proline tolerant tripeptidyl peptidases and uses thereof
CN107075534A (en) 2014-10-24 2017-08-18 丹尼斯科美国公司 The method that alcohol is prepared using three peptidyl peptidases
BR112018015589A2 (en) 2016-02-25 2019-10-01 Dupont Nutrition Biosci Aps A method for producing protein hydrolyzate employing an aspergillus fumigatus tripeptidyl peptidase
EP3491128B1 (en) * 2016-07-29 2021-09-08 New England Biolabs, Inc. Methods and compositions for preventing concatemerization during template- switching

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (en) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification of DNA sequences
EP0438292A2 (en) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Method of preparing primers for polymerase chain reaction and DNA amplification using said primers
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (en) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification of DNA sequences
EP0438292A2 (en) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Method of preparing primers for polymerase chain reaction and DNA amplification using said primers
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
G.P. SMITH: "The progeny of sexual PCR", NATURE, vol. 370, 4 August 1994 (1994-08-04), pages 324 - 325 *
PALERMO D.P.; HESS G.F.: "Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double stranded DNA", DNA, vol. 6, no. 3, pages 273 - 279 *
See also references of WO0058517A1 *
STEMMER W: "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, no. 22, 25 October 1994 (1994-10-25), pages 10747 - 10751, XP002170160 *
WAYNE M. COCO ET AL.: "DNA shuffling method for generating highly recombined genes and evolved enzymes", NATURE BIOTECHNOLOGY, vol. 19, no. 4, pages 354 - 359 *

Also Published As

Publication number Publication date
CA2329122A1 (en) 2000-10-05
EP1092041A1 (en) 2001-04-18
MXPA00011569A (en) 2004-12-03
WO2000058517A1 (en) 2000-10-05
JP2004500019A (en) 2004-01-08
AU4039400A (en) 2000-10-16

Similar Documents

Publication Publication Date Title
EP1092041A4 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
HUP0201438A2 (en) Dna vaccine - pcv
HUP0202221A1 (en) Tenascin-c nucleic acid lagands
EP1185698A4 (en) Automated nucleic acid compaction device
IL149491A0 (en) Nucleic acid-containing complex
DE60002723D1 (en) Propargylphenyl-äther a2a rezeptoragonisten
EP1125945A4 (en) Reversible photocoupling nucleic acid and phosphoroamidite
PL358811A1 (en) High order nucleic acid based structures
IL145165A0 (en) End selection in directed evolution
HUP0203848A3 (en) Nucleic acid vaccination
IL149679A0 (en) Nucleic acid construct and methods utilizing the same
GB9917875D0 (en) Nucleic acid constructs
GB9920816D0 (en) Plant gene
GB9905644D0 (en) Dna
GB9904994D0 (en) Improvements in roof panels
GB9911012D0 (en) Improvements in or relating to self-pierce riveting
AU5494700A (en) Rna metabolism proteins
GB0221295D0 (en) Improvements in or relating to nucleic acid amplification reactions
GB9927108D0 (en) Dna
EP1234032A4 (en) Novel nucleic acid and polypeptide
GB9912403D0 (en) Improvements in or relating to nucleic acid hybridisation
GB9822490D0 (en) Nucleic acid
GB9823481D0 (en) Nucleic acid
GB9900395D0 (en) Biological method
IL128483A0 (en) Nucleic acid molecules

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20001222

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

AX Request for extension of the european patent

Free format text: AL;LT;LV;MK;RO;SI

A4 Supplementary search report drawn up and despatched

Effective date: 20010806

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

17Q First examination report despatched

Effective date: 20020124

REG Reference to a national code

Ref country code: SE

Ref legal event code: TRCL

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20070630

R18D Application deemed to be withdrawn (corrected)

Effective date: 20070630