EP0677589A3 - Method, test element and test kit for semi-quantitative detection of target nucleic acid - Google Patents

Method, test element and test kit for semi-quantitative detection of target nucleic acid Download PDF

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Publication number
EP0677589A3
EP0677589A3 EP95302151A EP95302151A EP0677589A3 EP 0677589 A3 EP0677589 A3 EP 0677589A3 EP 95302151 A EP95302151 A EP 95302151A EP 95302151 A EP95302151 A EP 95302151A EP 0677589 A3 EP0677589 A3 EP 0677589A3
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
target nucleic
semi
test element
test
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP95302151A
Other languages
German (de)
French (fr)
Other versions
EP0677589B1 (en
EP0677589A2 (en
Inventor
Lynn Bergmeyer
Thomas Cummins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Priority to SI9530514T priority Critical patent/SI0677589T1/en
Publication of EP0677589A2 publication Critical patent/EP0677589A2/en
Publication of EP0677589A3 publication Critical patent/EP0677589A3/en
Application granted granted Critical
Publication of EP0677589B1 publication Critical patent/EP0677589B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.
EP95302151A 1994-03-31 1995-03-30 Method, test element and test kit for semi-quantitative detection of target nucleic acid Expired - Lifetime EP0677589B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SI9530514T SI0677589T1 (en) 1994-03-31 1995-03-30 Method, test element and test kit for semi-quantitative detection of target nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US221818 1994-03-31
US08/221,818 US5591580A (en) 1994-03-31 1994-03-31 Method, test element and test kit for semi-quantitative detection of target nucleic acid

Publications (3)

Publication Number Publication Date
EP0677589A2 EP0677589A2 (en) 1995-10-18
EP0677589A3 true EP0677589A3 (en) 1998-01-07
EP0677589B1 EP0677589B1 (en) 2001-05-16

Family

ID=22829523

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95302151A Expired - Lifetime EP0677589B1 (en) 1994-03-31 1995-03-30 Method, test element and test kit for semi-quantitative detection of target nucleic acid

Country Status (11)

Country Link
US (1) US5591580A (en)
EP (1) EP0677589B1 (en)
JP (1) JP3604758B2 (en)
AT (1) ATE201237T1 (en)
CA (1) CA2145957C (en)
DE (1) DE69520907T2 (en)
DK (1) DK0677589T3 (en)
ES (1) ES2156193T3 (en)
GR (1) GR3036316T3 (en)
PT (1) PT677589E (en)
SI (1) SI0677589T1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030148354A1 (en) * 1996-10-02 2003-08-07 Saftest, Inc. Devices and methods for isolating and detecting specific substances in complex matrices
KR100760221B1 (en) * 1997-11-28 2007-10-30 세로노 제네틱스 인스티튜트 에스.에이. Chlamydia trachomatis genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection
AU2777199A (en) * 1998-02-19 1999-09-06 Edvotek Articles of manufacture and methods for staining biomolecules
AU2002365053A1 (en) * 2001-12-20 2003-07-09 Safetest, Inc. Devices and methods for isolating and detecting specific substances in complex matrices
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US6868912B2 (en) * 2003-02-19 2005-03-22 Baker Hughes Incorporated Tension thrust ESPCP system

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0325449A2 (en) * 1988-01-19 1989-07-26 Idexx Laboratories, Inc. Immunoassays
WO1989010979A1 (en) * 1988-05-10 1989-11-16 E.I. Du Pont De Nemours And Company Process for rapid nucleic acid detection
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
WO1990008840A1 (en) * 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
EP0439222A2 (en) * 1990-01-26 1991-07-31 Johnson & Johnson Clinical Diagnostics, Inc. Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
WO1992016659A1 (en) * 1991-03-21 1992-10-01 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5273882A (en) * 1985-06-13 1993-12-28 Amgen Method and kit for performing nucleic acid hybridization assays
US5374524A (en) * 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
US5173260A (en) * 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
US5380489A (en) * 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
US5387510A (en) * 1991-10-02 1995-02-07 Eastman Kodak Company Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0325449A2 (en) * 1988-01-19 1989-07-26 Idexx Laboratories, Inc. Immunoassays
WO1989010979A1 (en) * 1988-05-10 1989-11-16 E.I. Du Pont De Nemours And Company Process for rapid nucleic acid detection
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
WO1990008840A1 (en) * 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
EP0439222A2 (en) * 1990-01-26 1991-07-31 Johnson & Johnson Clinical Diagnostics, Inc. Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
WO1992016659A1 (en) * 1991-03-21 1992-10-01 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes

Also Published As

Publication number Publication date
EP0677589B1 (en) 2001-05-16
DK0677589T3 (en) 2001-07-30
GR3036316T3 (en) 2001-10-31
CA2145957A1 (en) 1995-10-01
ES2156193T3 (en) 2001-06-16
JPH0850130A (en) 1996-02-20
EP0677589A2 (en) 1995-10-18
PT677589E (en) 2001-08-30
SI0677589T1 (en) 2002-06-30
US5591580A (en) 1997-01-07
JP3604758B2 (en) 2004-12-22
ATE201237T1 (en) 2001-06-15
CA2145957C (en) 2008-01-29
DE69520907D1 (en) 2001-06-21
DE69520907T2 (en) 2001-09-13

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