EP0271155A2 - Enzymatic dishwashing and rinsing process - Google Patents

Enzymatic dishwashing and rinsing process Download PDF

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Publication number
EP0271155A2
EP0271155A2 EP87202387A EP87202387A EP0271155A2 EP 0271155 A2 EP0271155 A2 EP 0271155A2 EP 87202387 A EP87202387 A EP 87202387A EP 87202387 A EP87202387 A EP 87202387A EP 0271155 A2 EP0271155 A2 EP 0271155A2
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Prior art keywords
weight
lipase
pseudomonas
composition according
dishwashing
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EP87202387A
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German (de)
French (fr)
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EP0271155B2 (en
EP0271155A3 (en
EP0271155B1 (en
Inventor
Willem Robert Van Dijk
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Unilever PLC
Unilever NV
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Unilever PLC
Unilever NV
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the present invention relates to an enzymatic dishwashing or rinsing composition comprising lipolytic enzymes.
  • Enzymatic dishwashing compositions have been proposed in the art. As enzymes, mainly amylases and/or proteases have been proposed for inclusion in dishwashing compositions. Lipases have also been suggested, but have received far less attention than the amylases and/or proteases. Dishwashing compositions, in particular machine dishwashing compositions which are used in the main wash step of a machine dishwashing operation, have in general a satisfactory cleaning performance. However, frequently the articles cleaned with such products still do not have a satisfactory visual appearance after rinsing and drying, showing film or spots.
  • main wash liquor is usually carried over from the main wash step to the rinse step, causing some deposition of soil resulting in insoluble calcium salts on the articles to be rinsed, which results in visible film or spots on the articles when they are dry. In particular with glass articles, this causes an unsightly visual appearance.
  • the lipases used according to the present invention, may be of any suitable origin such as yeasts, fungi and bacteria. Preferably, however, they are of bacterial origin, and particularly preferably they belong to the class of bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL B-3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division at Peoria, Illinois, under the number NRRL B-3673.
  • This lipase will hereinafter be referred to as "Toyo Jozo" lipase.
  • the preferred lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133 , pages 76-79 (1950)).
  • the preparation of the antiserum is carried out as follows: Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme: day 0 : antigen in complete Freund's adjuvant day 4 : antigen in complete Freund's adjuvant day 32 : antigen in incomplete Freund's adjuvant day 60 : booster of antigen in incomplete Freund's adjuvant.
  • the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • All lipases showing a positive immunological cross reaction with the Toyo Jozo lipase antibody as hereabove described are preferred lipases according to the present invention.
  • Typical examples thereof are the lipases ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp.
  • Chromobacter viscosum e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli .
  • the lipases of the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
  • lipases can be used in their nonpurified form, or in a purified form, e.g. purified with the aid of well-­known adsorption methods, such as a phenylsepharose-­adsorption techniques.
  • the composition of the invention may furthermore comprise the usual ingredients of dishwashing or rinse compositions.
  • it may contain one or more alkali salts commonly used in dishwashing compositions.
  • it may contain organic and/or inorganic builders such as the alkali metal ortho-, pyro and tripolyphosphates and hexametaphosphates, silicates, carbonates, zeolites, borates, citrates, carboxymethyloxy-­succinates, nitrilotriacetates and ethylenediamine-­tetraacetates, polymeric polyelectrolytes such as polyacrylates, polymaleates, and other known organic and inorganic builder compounds.
  • the amount of builders in the composition varies from 10-90% by weight, generally from 30-70% by weight.
  • the composition may also contain a detergent-active compound. If a detergent-active compound is included, it usually is in an amount of from 0.5-10%, usually 1-­5%. Any well-known type of detergent active compound may be used, such as soaps, synthetic anionic, non-­ionic, amphoteric detergent surfactant and mixtures thereof. Preferably, a nonionic detergent surfactant is used, especially a low-foaming one. Suitable examples of such nonionic detergent surfactants can easily be found in M.Schick "Nonionic Surfactants" (1967).
  • composition may furthermore contain other useful additives such as bleaching agents, bleaching agent activators, hydrotropes, fillers, perfumes, colouring agents, germicides, soil-suspending agents, aminopoly­phosphonic acids and alkali metal or alkaline earth metal salts thereof, clays such as hectorites, anti-­corrosion agents such as fatty acids, benztriazole and so on.
  • Other enzymes such as proteases, amylases, oxidases may also be included.
  • the peroxygen type bleaching agents preferably with a bleach precursor such as TAED are suitable for inclusion in the machine dishwashing compositions.
  • a typical example of a conventional machine dishwashing composition usually contains an alkali metal tripoly­phosphate in an amount of from 20-60%, an alkali metal silicate in an amount of from 40-80%, or an alkali metal disilicate in an amount of 5-30% by weight a peroxy type bleaching agent in an amount of from 1-15%, a low-foaming detergent surfactant in an amount of from 0.5-5%, and minor ingredients such as perfumes, colouring agents, hydrotropes, fillers, etc.
  • a rinse composition When formulated as a rinse composition, it may contain from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc., the balance being water.
  • a nonionic surfactant from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc.
  • the balance being water.
  • the products of the invention can be formulated in any desirable form, such as powders, granulates, cakes, bars, pastes, liquids etc. The invention will further be illustrated by way of example.
  • the detergent composition had the following formulation:
  • the Toyo Jozo lipase was used as lipase.
  • the test was carried out in two runs, one without and one with lipase.
  • the glasses were appraised by an expert panel as to their visual appearance, and were given a score according to the following scale:
  • Example 1 The test of Example 1 was repeated, but under the following different conditions: wash temperature : 65°C soil : standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
  • wash temperature 65°C soil : standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
  • run 1 no lipase added in the final rinse
  • run 2 2.5 g Toyo Jozo lipase was added to the final rinse (15 LU/ml).
  • the main wash composition had the following formulation:
  • Example 2 Replacing the Toyo Jozo lipase by the Amano-P lipase or Lipase MY (ex Candida cylindracea ) or Amano AP-6 lipase (ex Aspergillus niger ) in Example 2 gave similar results.

Abstract

The inclusion of lipases in machine dishwashing and rinsing compositions provides for a significant reduction of the spot and film formation on the dishware. Particularly the use of bacterial lipases, which show a positive immunological cross-reaction with the lipase, produced by Chromobacter viscosum var. lipolyticum NRRL B 3673 provides for such such significant reduction of spot and film formation.

Description

  • The present invention relates to an enzymatic dishwashing or rinsing composition comprising lipolytic enzymes.
  • Enzymatic dishwashing compositions have been proposed in the art. As enzymes, mainly amylases and/or proteases have been proposed for inclusion in dishwashing compositions. Lipases have also been suggested, but have received far less attention than the amylases and/or proteases. Dishwashing compositions, in particular machine dishwashing compositions which are used in the main wash step of a machine dishwashing operation, have in general a satisfactory cleaning performance. However, frequently the articles cleaned with such products still do not have a satisfactory visual appearance after rinsing and drying, showing film or spots. Some main wash liquor is usually carried over from the main wash step to the rinse step, causing some deposition of soil resulting in insoluble calcium salts on the articles to be rinsed, which results in visible film or spots on the articles when they are dry. In particular with glass articles, this causes an unsightly visual appearance.
  • We have now found that the addition of lipases to a main wash dishwashing composition or to a rinse composition significantly reduced the formation of film or spots on the articles cleaned or rinsed with such a composition. The lipases, used according to the present invention, may be of any suitable origin such as yeasts, fungi and bacteria. Preferably, however, they are of bacterial origin, and particularly preferably they belong to the class of bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL B-3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division at Peoria, Illinois, under the number NRRL B-3673. This lipase will hereinafter be referred to as "Toyo Jozo" lipase. The preferred lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Ouchterlony (Acta. Med. Scan., 133, pages 76-79 (1950)).
  • The preparation of the antiserum is carried out as follows:
    Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme:
    day 0 : antigen in complete Freund's adjuvant
    day 4 : antigen in complete Freund's adjuvant
    day 32 : antigen in incomplete Freund's adjuvant
    day 60 : booster of antigen in incomplete Freund's adjuvant.
  • The serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • The titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 2⁵ dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • All lipases showing a positive immunological cross reaction with the Toyo Jozo lipase antibody as hereabove described are preferred lipases according to the present invention. Typical examples thereof are the lipases ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P 1338, the lipase ex Pseudomonas sp. available under the trade name Amano-CES, the lipase ex Pseudomonas cepacia, lipases ex Chromobacter viscosum, e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., U.S.A. and Diosynth Co., The Netherlands, and lipases ex Pseudomonas gladioli.
  • The lipases of the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg preferably 25 to 0.05 LU/mg of the composition.
  • A Lipase Unit (LU) is that amount of lipase which produces 1µmol of titratable fatty acid per minute in a pH stat. under the following conditions:
    temperature 30°C; pH = 9.0; substrate is an emulsion of 3.3 wt.% of olive oil and 3.3% gum arabic, in the presence of 13 mmol/l Ca²⁺ and 20 mmol/l NaCl in 5 mmol/l Tris-buffer.
  • Naturally, mixtures of the above lipases can be used. The lipases can be used in their nonpurified form, or in a purified form, e.g. purified with the aid of well-­known adsorption methods, such as a phenylsepharose-­adsorption techniques.
  • The composition of the invention may furthermore comprise the usual ingredients of dishwashing or rinse compositions. Thus it may contain one or more alkali salts commonly used in dishwashing compositions. Thus, it may contain organic and/or inorganic builders such as the alkali metal ortho-, pyro and tripolyphosphates and hexametaphosphates, silicates, carbonates, zeolites, borates, citrates, carboxymethyloxy-­succinates, nitrilotriacetates and ethylenediamine-­tetraacetates, polymeric polyelectrolytes such as polyacrylates, polymaleates, and other known organic and inorganic builder compounds.
  • Usually, the amount of builders in the composition varies from 10-90% by weight, generally from 30-70% by weight.
  • The composition may also contain a detergent-active compound. If a detergent-active compound is included, it usually is in an amount of from 0.5-10%, usually 1-­5%. Any well-known type of detergent active compound may be used, such as soaps, synthetic anionic, non-­ionic, amphoteric detergent surfactant and mixtures thereof. Preferably, a nonionic detergent surfactant is used, especially a low-foaming one. Suitable examples of such nonionic detergent surfactants can easily be found in M.Schick "Nonionic Surfactants" (1967).
  • The composition may furthermore contain other useful additives such as bleaching agents, bleaching agent activators, hydrotropes, fillers, perfumes, colouring agents, germicides, soil-suspending agents, aminopoly­phosphonic acids and alkali metal or alkaline earth metal salts thereof, clays such as hectorites, anti-­corrosion agents such as fatty acids, benztriazole and so on. Other enzymes such as proteases, amylases, oxidases may also be included.
  • As bleaching agents the peroxygen type bleaching agents, preferably with a bleach precursor such as TAED are suitable for inclusion in the machine dishwashing compositions.
  • A typical example of a conventional machine dishwashing composition usually contains an alkali metal tripoly­phosphate in an amount of from 20-60%, an alkali metal silicate in an amount of from 40-80%, or an alkali metal disilicate in an amount of 5-30% by weight a peroxy type bleaching agent in an amount of from 1-15%, a low-foaming detergent surfactant in an amount of from 0.5-5%, and minor ingredients such as perfumes, colouring agents, hydrotropes, fillers, etc.
  • When formulated as a rinse composition, it may contain from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an inorganic or organic acid, from 1-5% by weight of a hydrotrope, dyes, germicides, clays, silica sols, etc., the balance being water. The products of the invention can be formulated in any desirable form, such as powders, granulates, cakes, bars, pastes, liquids etc. The invention will further be illustrated by way of example.
    • Example 1
  • In a commercial dishwashing machine tests were carried out under the following conditions:
    detergent composition dosage: 30 g
    lipase dosage               : 2.5 g (= 15 LU/ml)
    water hardness              :   main wash 27° GH
                                  flood        9° GH
                                  rinse 1      9° GH
                                  rinse 2      9° GH
                                  final rinse  9° GH
    wash temperature and time    : 55°C - 60 minutes
    articles to be cleaned       : clean dishes
                                   clean glasses
    test soil      : 35 g egg-yolk, added at the beginning of the main wash.
  • The detergent composition had the following formulation:
    Figure imgb0001
  • As lipase, the Toyo Jozo lipase was used.
  • The test was carried out in two runs, one without and one with lipase.
  • The glasses were appraised by an expert panel as to their visual appearance, and were given a score according to the following scale:
    Figure imgb0002
  • The following results were obtained:
    Figure imgb0003
    • Example 2
  • The test of Example 1 was repeated, but under the following different conditions:

    wash temperature      : 65°C
    soil      : standard soil; at the end of the main wash, clean plates were put in the machine, so that no cleaning took place in the final rinse, but only a carry-over took place.
    run 1      : no lipase added in the final rinse
    run 2      : 2.5 g Toyo Jozo lipase was added to the final rinse (15 LU/ml).
  • The main wash composition had the following formulation:
    Figure imgb0004
  • The following spot and film scores of the glasses were obtained:
    Figure imgb0005
    • Example 3
  • Replacing the Toyo Jozo lipase by the Amano-P lipase or Lipase MY (ex Candida cylindracea) or Amano AP-6 lipase (ex Aspergillus niger) in Example 2 gave similar results.
    • Example 4
  • The following formulation is an example of a rinse aid composition to which lipases can be added:
    Figure imgb0006

Claims (5)

1. An enzymatic dishwashing or rinsing composition comprising from 0.5-10% by weight of a detergent surfactant and from 0-90% by weight of a builder, characterised in that it further comprises from 0.005-100 LU/mg of a lipolytic enzyme.
2. A composition according to claim 1, characterised in that the lipase is a bacterial lipase which shows a positive immunological cross-reaction with the antibody of the lipase, produced by Chromobacter viscosum var. lipolyticum NRRL B 3673.
3. A composition according to claim 2, characterised in that the lipase is producible by the microorganism selected from the group consisting of Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas nitroreducens var. lipolyticum, Pseudomonas cepacia, Pseudomonas gladioli and Chromobacter viscosum.
4. A dishwashing composition according to claims 1-3, characterised in that it contains from 20-60% by weight of an alkali metal tripolyphosphate, from 40-80% by weight of an alkali metal silicate, from 1-15% by weight of a peroxy type bleaching agent and from 0.5-5% by weight of a low-foaming nonionic detergent.
5. A rinsing composition according to claims 1-3, characterised in that it contains, in an aqueous medium, from 0.5-10% by weight of a nonionic surfactant, from 5-25% by weight of an organic or inorganic acid, and from 1-5% by weight of a hydrotrope.
EP87202387A 1986-12-10 1987-12-02 Enzymatic dishwashing and rinsing process Expired - Lifetime EP0271155B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8629538 1986-12-10
GB868629538A GB8629538D0 (en) 1986-12-10 1986-12-10 Enzymatic dishwashing & rinsing composition

Publications (4)

Publication Number Publication Date
EP0271155A2 true EP0271155A2 (en) 1988-06-15
EP0271155A3 EP0271155A3 (en) 1988-08-17
EP0271155B1 EP0271155B1 (en) 1991-09-04
EP0271155B2 EP0271155B2 (en) 2000-09-06

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EP (1) EP0271155B2 (en)
JP (1) JPS63161086A (en)
AU (1) AU609755B2 (en)
BR (1) BR8706685A (en)
DE (1) DE3772726D1 (en)
ES (1) ES2025635T5 (en)
GB (1) GB8629538D0 (en)
ZA (1) ZA879297B (en)

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EP0346137A1 (en) * 1988-06-09 1989-12-13 Unilever Plc Enzymatic dishwashing composition
WO1989012090A1 (en) * 1988-06-09 1989-12-14 Unilever Plc Enzymatic dishwashing and rinsing composition
US4933287A (en) * 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
EP0425214A2 (en) * 1989-10-23 1991-05-02 Unilever Plc Enzyme-containing detergent compositions and their use
GB2247025A (en) * 1990-08-13 1992-02-19 Unilever Plc Enzymatic dishwashing and rinsing composition
US5100796A (en) * 1988-02-22 1992-03-31 Synfina-Oleofina Methods for producing a new pseudomonas lipase and protease and detergent washing compositions containing same
DE4324202A1 (en) * 1993-05-25 1994-12-01 Henkel Ecolab Gmbh & Co Ohg Process and apparatus for mechanical dishwashing
EP0712438A1 (en) * 1993-08-10 1996-05-22 The Procter & Gamble Company Manual dishwashing composition comprising lipase enzymes
US5527484A (en) * 1991-05-31 1996-06-18 Colgate-Palmolive Co. Phosphate containing powdered automatic dishwashing composition with enzymes
WO1997008281A1 (en) * 1995-08-25 1997-03-06 Henkel Kommanditgesellschaft Auf Aktien Use of lipases in low-alkaline mechanical dishwashing agents
EP0700265B1 (en) * 1993-05-25 1997-07-30 Henkel-Ecolab GmbH & Co. OHG Machine dish-washing process and device
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
WO2011049945A2 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
EP2428572A2 (en) 2007-03-09 2012-03-14 Danisco US, Inc., Genencor Division Alkaliphilic Bacillus species alpha-amylase variants, compositions comprising alpha-amylase variants, and methods of use
US8323945B2 (en) 2008-06-06 2012-12-04 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof
US8507243B2 (en) 2008-09-25 2013-08-13 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
US9040278B2 (en) 2008-06-06 2015-05-26 Danisco Us Inc. Production of glucose from starch using alpha-amylases from Bacillus subtilis
US9040279B2 (en) 2008-06-06 2015-05-26 Danisco Us Inc. Saccharification enzyme composition and method of saccharification thereof
WO2017220422A1 (en) * 2016-06-23 2017-12-28 Novozymes A/S Use of enzymes, composition and method for removing soil

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JPH0277498A (en) * 1988-09-14 1990-03-16 Lion Corp Detergent composition for use in automatic dishwasher
US4908150A (en) * 1989-02-02 1990-03-13 Lever Brothers Company Stabilized lipolytic enzyme-containing liquid detergent composition
JPH02245129A (en) * 1989-03-17 1990-09-28 Novo Nordisk As Enzymatically removing method for stain on stock
JP2620384B2 (en) * 1989-12-20 1997-06-11 花王株式会社 Dishwashing composition for automatic dishwashers
JPH0699715B2 (en) * 1990-07-11 1994-12-07 花王株式会社 Detergent composition for automatic dishwasher
JP5246300B2 (en) * 2011-06-14 2013-07-24 Ｊｆｅスチール株式会社 Body side structure

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US4933287A (en) * 1985-08-09 1990-06-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
US5100796A (en) * 1988-02-22 1992-03-31 Synfina-Oleofina Methods for producing a new pseudomonas lipase and protease and detergent washing compositions containing same
WO1989012089A1 (en) * 1988-06-09 1989-12-14 Unilever Plc Enzymatic dishwashing composition
WO1989012090A1 (en) * 1988-06-09 1989-12-14 Unilever Plc Enzymatic dishwashing and rinsing composition
EP0346137A1 (en) * 1988-06-09 1989-12-13 Unilever Plc Enzymatic dishwashing composition
EP0425214A2 (en) * 1989-10-23 1991-05-02 Unilever Plc Enzyme-containing detergent compositions and their use
EP0425214A3 (en) * 1989-10-23 1992-05-06 Unilever Plc Enzyme-containing detergent compositions and their use
GB2247025A (en) * 1990-08-13 1992-02-19 Unilever Plc Enzymatic dishwashing and rinsing composition
US5527484A (en) * 1991-05-31 1996-06-18 Colgate-Palmolive Co. Phosphate containing powdered automatic dishwashing composition with enzymes
EP0700265B1 (en) * 1993-05-25 1997-07-30 Henkel-Ecolab GmbH & Co. OHG Machine dish-washing process and device
DE4324202A1 (en) * 1993-05-25 1994-12-01 Henkel Ecolab Gmbh & Co Ohg Process and apparatus for mechanical dishwashing
US6718991B1 (en) 1993-05-25 2004-04-13 Ecolab Gmbh & Co. Ohg Process and an arrangement for machine dishwashing
EP0712438A4 (en) * 1993-08-10 1999-04-14 Procter & Gamble Manual dishwashing composition comprising lipase enzymes
EP0712438A1 (en) * 1993-08-10 1996-05-22 The Procter & Gamble Company Manual dishwashing composition comprising lipase enzymes
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
WO1997008281A1 (en) * 1995-08-25 1997-03-06 Henkel Kommanditgesellschaft Auf Aktien Use of lipases in low-alkaline mechanical dishwashing agents
EP2428572A2 (en) 2007-03-09 2012-03-14 Danisco US, Inc., Genencor Division Alkaliphilic Bacillus species alpha-amylase variants, compositions comprising alpha-amylase variants, and methods of use
US8323945B2 (en) 2008-06-06 2012-12-04 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof
US8975056B2 (en) 2008-06-06 2015-03-10 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof
US9040278B2 (en) 2008-06-06 2015-05-26 Danisco Us Inc. Production of glucose from starch using alpha-amylases from Bacillus subtilis
US9040279B2 (en) 2008-06-06 2015-05-26 Danisco Us Inc. Saccharification enzyme composition and method of saccharification thereof
US9090887B2 (en) 2008-06-06 2015-07-28 Danisco Us Inc. Variant alpha-amylases from Bacillus subtilis and methods of use, thereof
US8507243B2 (en) 2008-09-25 2013-08-13 Danisco Us Inc. Alpha-amylase blends and methods for using said blends
WO2011049945A2 (en) 2009-10-23 2011-04-28 Danisco Us Inc. Methods for reducing blue saccharide
WO2017220422A1 (en) * 2016-06-23 2017-12-28 Novozymes A/S Use of enzymes, composition and method for removing soil
US11001787B2 (en) 2016-06-23 2021-05-11 Novozymes A/S Use of enzymes, composition and method for removing soil

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EP0271155B2 (en) 2000-09-06
ZA879297B (en) 1989-08-30
GB8629538D0 (en) 1987-01-21
EP0271155A3 (en) 1988-08-17
AU609755B2 (en) 1991-05-09
JPS63161086A (en) 1988-07-04
ES2025635T5 (en) 2001-01-16
DE3772726D1 (en) 1991-10-10
BR8706685A (en) 1988-07-19
ES2025635T3 (en) 1992-04-01
EP0271155B1 (en) 1991-09-04
AU8222387A (en) 1988-06-16

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