EA200000029A1 - EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS - Google Patents

EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS

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Publication number
EA200000029A1
EA200000029A1 EA200000029A EA200000029A EA200000029A1 EA 200000029 A1 EA200000029 A1 EA 200000029A1 EA 200000029 A EA200000029 A EA 200000029A EA 200000029 A EA200000029 A EA 200000029A EA 200000029 A1 EA200000029 A1 EA 200000029A1
Authority
EA
Eurasian Patent Office
Prior art keywords
leaves
fda
plants
fructose
sucrose
Prior art date
Application number
EA200000029A
Other languages
Russian (ru)
Inventor
Джерард Ф. Барри
Нордайн Чеих
Ганеш М. Кишор
Original Assignee
Монсанто Компани
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Монсанто Компани filed Critical Монсанто Компани
Publication of EA200000029A1 publication Critical patent/EA200000029A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Фруктозо-1,6-бисфосфатальдолаза (FDA) является ферментом, обратимо катализирующим реакцию превращения триозофосфата в фруктозо-1,6-бисфосфат. В листьях этот фермент расположен в хлоропластах (синтез крахмала) и в цитозоле (биосинтез сахарозы). Созданы трансгенные растения, которые экспрессируют fda E. coli в хлоропластах для повышения выхода растений за счет повышения способности листьев к биосинтезу крахмала в частности, и продуцированию сахарозы в целом. Листья растений, экспрессирующих трансген fda, демонстрируют значительно более высокое накопление крахмала по сравнению с контрольными растениями, экспрессирующими нулевой вектор, особенно в начале фотопериода, но содержат в листьях меньше сахарозы. Трансгенные растения обладают также значительно большей корневой массой. Кроме того, трансгенный картофель, экспрессирующий fda, демонстрирует повышенную равномерность распределения твердых веществ.Международная заявка была опубликована вместе с отчетом о международном поиске.Fructose-1,6-bisphosphate-aldolase (FDA) is an enzyme that reversibly catalyzes the conversion of triosophosphate to fructose-1,6-bisphosphate. In leaves, this enzyme is located in chloroplasts (starch synthesis) and in the cytosol (sucrose biosynthesis). Transgenic plants that express E. coli fda in chloroplasts are created to increase the yield of plants by increasing the ability of the leaves to starch biosynthesis in particular, and to produce sucrose as a whole. Leaves of plants expressing the fda transgene show a significantly higher starch accumulation compared to control plants expressing the zero vector, especially at the beginning of the photoperiod, but contain less sucrose in the leaves. Transgenic plants also have a significantly greater root mass. In addition, transgenic potatoes expressing fda show an increased uniform distribution of solids. The international application was published along with an international search report.

EA200000029A 1997-06-17 1998-06-16 EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS EA200000029A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4995597P 1997-06-17 1997-06-17
PCT/US1998/012447 WO1998058069A1 (en) 1997-06-17 1998-06-16 Expression of fructose 1,6 bisphosphate aldolase in transgenic plants

Publications (1)

Publication Number Publication Date
EA200000029A1 true EA200000029A1 (en) 2000-08-28

Family

ID=21962657

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200000029A EA200000029A1 (en) 1997-06-17 1998-06-16 EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS

Country Status (16)

Country Link
EP (1) EP1002114A1 (en)
JP (1) JP2001520522A (en)
CN (1) CN1267333A (en)
AR (1) AR020307A1 (en)
AU (1) AU735383B2 (en)
BR (1) BR9810171A (en)
CA (1) CA2294525A1 (en)
EA (1) EA200000029A1 (en)
HU (1) HUP0004228A3 (en)
IL (1) IL133532A0 (en)
NO (1) NO996218L (en)
NZ (1) NZ501781A (en)
PL (1) PL337581A1 (en)
TR (1) TR199903157T2 (en)
WO (1) WO1998058069A1 (en)
ZA (1) ZA985261B (en)

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US6512162B2 (en) 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
ES2286886T3 (en) 1998-07-10 2007-12-01 Calgene Llc EXPRESSION OF EUCARIOUS PEPTIDES IN PLASTIDES OF PLANTS.
US7868229B2 (en) 1999-03-23 2011-01-11 Mendel Biotechnology, Inc. Early flowering in genetically modified plants
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
US7858848B2 (en) 1999-11-17 2010-12-28 Mendel Biotechnology Inc. Transcription factors for increasing yield
JP3357909B2 (en) 1999-03-10 2002-12-16 奈良先端科学技術大学院大学長 How to increase productivity of higher plants
EP1229781B1 (en) * 1999-11-17 2013-01-02 Mendel Biotechnology, Inc. Seed trait genes
JP5111706B2 (en) 1999-12-16 2013-01-09 モンサント テクノロジー エルエルシー DNA constructs for the expression of heterologous polypeptides in plants
EP1417318B1 (en) 2000-10-30 2011-05-11 Monsanto Technology LLC Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
US7939715B2 (en) 2000-11-16 2011-05-10 Mendel Biotechnology, Inc. Plants with improved yield and stress tolerance
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
JP2005130770A (en) 2003-10-30 2005-05-26 Ajinomoto Co Inc Potato increased in starch level obtained per plant body and method for creating the same
CN104178511A (en) * 2004-07-31 2014-12-03 梅坦诺米克斯有限公司 Preparation of organisms with faster growth and/or higher yield
AU2006314188B2 (en) * 2005-11-16 2014-01-16 Pro Natura Gesellschaft Fur Gesunde Ernahrung Mbh Agent for use in the case of fructose intolerance
CN101378652B (en) * 2006-02-09 2013-03-20 独立行政法人科学技术振兴机构 Plant having improved growth ability and disease resistance and method for production thereof
CN102268430B (en) * 2010-06-02 2013-05-15 中国科学院大连化学物理研究所 Fructose-1,6-diphosphate aldolase promoter and application thereof, construct and vector
ES2842501T5 (en) 2015-09-21 2023-04-13 Modern Meadow Inc Fiber Reinforced Fabric Composite Materials
ES2807727T3 (en) 2016-02-15 2021-02-24 Modern Meadow Inc Biofabricated composite material
CN105973851B (en) * 2016-06-12 2018-10-23 中国科学院地球化学研究所 A kind of bathmic method of quantitative determination plant photosynthesis
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
US11352497B2 (en) 2019-01-17 2022-06-07 Modern Meadow, Inc. Layered collagen materials and methods of making the same
CN112094859B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoFBA gene, expression vector, preparation method and application thereof
CN112094851B (en) * 2020-09-25 2022-01-25 扬州大学 Paeonia ostii PoCAB151 gene, expression vector, and preparation method and application thereof
CN113528567B (en) * 2021-07-22 2022-05-03 中国林业科学研究院林业研究所 Use of FBA8 protein or protein derived therefrom for regulating vascular bundle division and/or rachis cross-sectional area in plants
CN113528497B (en) * 2021-07-22 2022-06-24 中国林业科学研究院林业研究所 Use of FBA8 gene or FBA8 protein for preparing reagent with transphosphorylation activity and/or proteolytic activity
CN114920938B (en) * 2022-05-29 2023-11-10 深圳绿天琪生物医药有限公司 Preparation and application of arginine coupled plant source glycan and polypeptide synthetic copolymer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3325022B2 (en) * 1990-06-18 2002-09-17 モンサント カンパニー Increased starch content in plants
DE19502053A1 (en) * 1995-01-13 1996-07-18 Inst Genbiologische Forschung Processes and DNA molecules to increase the rate of photosynthesis in plants, as well as plant cells and plants with an increased rate of photosynthesis
US5716837A (en) * 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants

Also Published As

Publication number Publication date
HUP0004228A3 (en) 2002-11-28
AU735383B2 (en) 2001-07-05
AR020307A1 (en) 2002-05-08
BR9810171A (en) 2001-11-06
NO996218L (en) 2000-02-14
CN1267333A (en) 2000-09-20
WO1998058069A1 (en) 1998-12-23
EP1002114A1 (en) 2000-05-24
ZA985261B (en) 1999-01-07
NZ501781A (en) 2001-07-27
JP2001520522A (en) 2001-10-30
PL337581A1 (en) 2000-08-28
NO996218D0 (en) 1999-12-15
TR199903157T2 (en) 2000-06-21
IL133532A0 (en) 2001-04-30
AU8144598A (en) 1999-01-04
CA2294525A1 (en) 1998-12-23
HUP0004228A2 (en) 2001-03-28

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