DK3072535T5 - Fremgangsmåde til rekonstruktion af hud - Google Patents

Fremgangsmåde til rekonstruktion af hud Download PDF

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DK3072535T5
DK3072535T5 DK15305441.6T DK15305441T DK3072535T5 DK 3072535 T5 DK3072535 T5 DK 3072535T5 DK 15305441 T DK15305441 T DK 15305441T DK 3072535 T5 DK3072535 T5 DK 3072535T5
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skin
medium
substitute
fibroblasts
culture
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DK15305441.6T
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DK3072535T3 (da
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Vincent Casoli
Muriel Cario-André
Jean-Christophe Lepivert
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Univ Bordeaux
Inst Nat De La Santé Et De La Rech Médicale (Inserm)
Centre Hospitalier Univ Bordeaux
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/905—Hyaluronic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/091—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells melanocytes
    • C—CHEMISTRY; METALLURGY
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
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  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Claims (14)

  1. FREMGANGSMÅDE TIL REKONSTRUKTION AF HUD
    1. Fremgangsmåde til fremstilling af en hudsubstitut omfattende følgende trin: a. dyrkning af fibroblaster i et fibroblastdyrkningsmedium Ml; b. podning af en matrix omfattende collagen med fibroblaster opnået fra trin a; c. dyrkning af indpodede fibroblaster i en matrix omfattende collagen i et fibroblastdyrkningsmedium M2, hvilken matrix og de dyrkede fibroblaster danner en dermissubstitut; d. dyrkning af melanocytter i et melanocytdyrkningsmedium M3; e. dyrkning af keratinocytter i et keratinocytdyrkningsmedium M4 ; f. blanding af melanocytter opnået i trin d med keratinocytter opnået i trin e; g. podning af dermissubstitutten opnået i trin c med blandingen opnået i trin f; h. dyrkning af dermissubstitut indpodet i trin g i et hudkulturmedium M5, således at hudsubstitutten dannes. hvori mediet M2 omfatter ascorbinsyre eller en ascorbat, mediet M5 omfatter hyaluronsyre eller en hyaluronat eller et derivat af disse produkter, og podning i trin g udføres med et (keratinocytter + melanocytter)/fibroblaster-forhold fra 9 og 19.
  2. 2. Fremgangsmåde ifølge krav 1, hvori mediet M5 omfatter ascorbinsyre eller en ascorbat.
  3. 3. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 2, hvori blandingen af melanocytter og keratinocytter i trin f foretages med et melanocytter / keratinocytter-forhold fra 1/20 og 1/15.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori podning udført i trin b udføres ved en densitet på mellem 20.000 og 50.000 fibroblaster / cm2 af overfladearealet af den matrixen omfattende collagen.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori trin c. omfatter et første dyrkningstrin c' i 18 til 28 timer i tilstedeværelse af et fibroblastdyrkningsmedium M21 omfattende hverken ascorbinsyre eller ascorbat, et andet dyrkningstrin c" på mindst 2 dage i tilstedeværelse af et fibroblastdyrkningsmedium M22 omfattende ascorbinsyre eller en ascorbat.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvori trin h. omfatter: - et første 6- til 24-timers dyrkningstrin h.' i tilstedeværelse af et dyrkningsmedium M51 omfattende hverken hyaluronsyre eller hyaluronat eller ascorbinsyre eller ascorbat, - et andet dyrkningstrin h." på mindst 2 dage i tilstedeværelse af et dyrkningsmedium M52 omfattende hyaluronsyre eller en hyaluronat og - et tredje dyrkningstrin h.'" på mindst 2 dage i et medium M53 omfattende hyaluronsyre eller en hyaluronat og ascorbinsyre eller en ascorbat.
  7. 7. Hudsubstitut opnået ved implementering af fremgangsmåden defineret ved et hvilket som helst af de foregående krav.
  8. 8. Hudsubstitut ifølge krav 7, hvori fibroblasterne er autologe fra et individ under forberedelse til en graft af hudsubstitutten på individet.
  9. 9. Hudsubstitut ifølge krav 8, hvori fibroblasterne, melanocytterne og keratinocytterne er autologe fra et individ.
  10. 10. Graft udgjort af en hudsubstitut ifølge krav 8 eller 9.
  11. 11. Graft ifølge krav 10 til anvendelse som en behandlingsmetode for en hudlidelse og/eller et tab af hudsubstans.
  12. 12. Graft til anvendelse ifølge krav 11, hvori hudlidelsen og/eller tabet af hudsubstans er udvalgt fra gruppen bestående af en forbrænding, en helingsdefekt, et kronisk sår, en pigmentforstyrrelse, at hæmangiom og en hudcancer.
  13. 13. Kit til anvendelse af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 6 omfattende et fibroblastdyrkningsmedium Ml, en matrix omfattende collagen, et fibroblastdyrkningsmedium M2 i matrixen omfattende collagen, et melanocytdyrkningsmedium M3, et keratinocytdyrkningsmedium M4 og et huddyrkningsmedium M5, hvori mediet M2 omfatter ascorbinsyre eller en ascorbat, og mediet M5 omfatter hyaluronsyre eller en hyaluronat.
  14. 14. Kit ifølge krav 13, hvori medierne Ml til M5 uafhængigt er medier af klinisk kvalitet.
DK15305441.6T 2015-03-26 2015-03-26 Fremgangsmåde til rekonstruktion af hud DK3072535T5 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15305441.6A EP3072535B1 (fr) 2015-03-26 2015-03-26 Procédé de reconstruction de peau

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DK3072535T3 DK3072535T3 (da) 2017-05-22
DK3072535T5 true DK3072535T5 (da) 2017-06-06

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US (1) US11643639B2 (da)
EP (2) EP3072535B1 (da)
JP (1) JP6729880B2 (da)
CN (1) CN107683148B (da)
AU (1) AU2016238760B2 (da)
BR (1) BR112017020013B1 (da)
CA (1) CA2977725A1 (da)
DK (2) DK3072535T5 (da)
ES (2) ES2625768T3 (da)
WO (1) WO2016151133A1 (da)

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KR102540981B1 (ko) * 2017-06-30 2023-06-08 (주)아모레퍼시픽 인공피부 제조방법 및 인공피부
CN111450321A (zh) * 2019-01-18 2020-07-28 北京华弗生物科技有限公司 人工皮肤替代物及其无支架自组装构建方法和用途
KR102460180B1 (ko) * 2020-11-27 2022-10-27 숭실대학교산학협력단 연령별 구조 및 물성을 갖는 인공피부, 및 이의 제조방법
CN115627256B (zh) * 2022-12-02 2023-03-07 上海尚瑞生物医药科技有限公司 毛囊细胞构成的多层组织工程皮肤及其制备方法与应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0282746A1 (en) * 1987-02-19 1988-09-21 Takeda Chemical Industries, Ltd. Method for producing artificial cultured tissue
CA2119064A1 (en) * 1993-03-17 1994-09-18 Richard A. Berg Dermal-epidermal in vitro test system
IT1281870B1 (it) * 1995-04-27 1998-03-03 Fidia Advanced Biopolymers Srl Pelle artificiale umana costituita da materiali biocompatibili a base di derivati dell'acido ialuronico
FR2737971B1 (fr) * 1995-08-25 1997-11-14 Lvmh Rech Utilisation de la vitamine c ou de ses derives ou analogues pour stimuler la synthese de l'elastine cutanee
US6451601B1 (en) * 1999-04-12 2002-09-17 Modex Therapeutiques, S.A. Transiently immortalized cells for use in gene therapy
JP3738273B2 (ja) * 1999-07-20 2006-01-25 ディーエフビー ファーマシューティカルズ， インコーポレイテッド 改良ケラチノサイト培養物およびその使用
ATE308610T1 (de) * 2000-05-31 2005-11-15 Fraunhofer Ges Forschung Dreidimensionales hautmodell
US7741116B2 (en) * 2002-03-06 2010-06-22 University Of Cincinnati Surgical device for skin therapy or testing
CA2485567A1 (en) * 2002-05-10 2003-11-20 Isolagen Technologies, Inc. Treatments with autologous fibroblast
FR2856891B1 (fr) * 2003-07-04 2007-09-07 Stem Alpha Milieu de conservation d'organes, de tissus biologiques ou de cellules vivantes
KR100806695B1 (ko) * 2005-11-25 2008-02-27 주식회사 엠씨티티 색소화 질환 치료용 세포치료 약제학적 조성물
EP1972685B1 (en) * 2007-03-20 2015-09-02 Université Paris Descartes Culture medium for gingival fibroblasts
CA2658074C (fr) 2008-03-17 2017-11-07 L'oreal Equivalent de peau pigmentee fonctionnelle
US9173969B2 (en) * 2012-04-11 2015-11-03 Kaohsiung Medical University Biomaterial for wound healing
EP2991692B1 (en) * 2013-05-03 2019-08-21 The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. Skin substitutes and methods for hair follicle neogenesis

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ES2772700T3 (es) 2020-07-08
AU2016238760B2 (en) 2019-11-14
CA2977725A1 (fr) 2016-09-29
BR112017020013A2 (pt) 2018-06-19
WO2016151133A1 (fr) 2016-09-29
ES2625768T3 (es) 2017-07-20
JP2018513724A (ja) 2018-05-31
US11643639B2 (en) 2023-05-09
EP3274005A1 (fr) 2018-01-31
JP6729880B2 (ja) 2020-07-29
DK3072535T3 (da) 2017-05-22
CN107683148A (zh) 2018-02-09
BR112017020013B1 (pt) 2021-06-22
CN107683148B (zh) 2021-05-04
EP3072535A1 (fr) 2016-09-28
EP3274005B1 (fr) 2019-12-04
EP3072535B1 (fr) 2017-03-01
AU2016238760A1 (en) 2017-09-14
US20180112188A1 (en) 2018-04-26
DK3274005T3 (da) 2020-02-24

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