DK0387775T3 - Genetic unit for inhibiting the function of RNA - Google Patents

Genetic unit for inhibiting the function of RNA

Info

Publication number
DK0387775T3
DK0387775T3 DK90104701.9T DK90104701T DK0387775T3 DK 0387775 T3 DK0387775 T3 DK 0387775T3 DK 90104701 T DK90104701 T DK 90104701T DK 0387775 T3 DK0387775 T3 DK 0387775T3
Authority
DK
Denmark
Prior art keywords
rna
units
inhibiting
unit
genetic
Prior art date
Application number
DK90104701.9T
Other languages
Danish (da)
Inventor
Hartmut Dr Beug
Max L Prof-Dr Birnstiel
Matthew Dr Cotten
Ernst Dr Wagner
Harald Dipl-Ing Kandolf
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Application granted granted Critical
Publication of DK0387775T3 publication Critical patent/DK0387775T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to a genetic unit, which is optionally in multicopy form, for the inhibition of RNA. The unit contains the transcription units necessary for transcription by polymerase III, and a DNA which codes for inhibiting RNA and which is arranged within the unit in such a way that the transcribed RNA is part of the polymerase III transcript. These units can be used to achieve increased stability of the inhibiting RNA, which can be in the form of ribozymes or antisense RNAs, while there is no reduction in the activity. The invention furthermore relates to a process for introducing the genetic units into the cell, to the use of these units and to pharmaceutical formulations containing them.
DK90104701.9T 1989-03-16 1990-03-13 Genetic unit for inhibiting the function of RNA DK0387775T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT60989 1989-03-16

Publications (1)

Publication Number Publication Date
DK0387775T3 true DK0387775T3 (en) 1996-11-04

Family

ID=3495118

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90104701.9T DK0387775T3 (en) 1989-03-16 1990-03-13 Genetic unit for inhibiting the function of RNA

Country Status (20)

Country Link
EP (1) EP0387775B1 (en)
JP (1) JP3330930B2 (en)
KR (1) KR900014591A (en)
AT (1) ATE140961T1 (en)
AU (1) AU637354B2 (en)
CA (1) CA2012312C (en)
DD (1) DD297838A5 (en)
DE (1) DE59010432D1 (en)
DK (1) DK0387775T3 (en)
ES (1) ES2090049T3 (en)
FI (1) FI105103B (en)
GR (1) GR3020935T3 (en)
HU (2) HU215187B (en)
IE (1) IE74850B1 (en)
IL (1) IL93754A (en)
NO (1) NO306623B1 (en)
NZ (1) NZ232917A (en)
PT (1) PT93440B (en)
RU (1) RU2136751C1 (en)
ZA (1) ZA901973B (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0471796A4 (en) * 1989-05-10 1993-05-05 Sloan Kettering Institute For Cancer Research Stably transformed eucaryotic cells comprising a foreign transcribable dna under the control of a pol iii promoter
DE4091533T (en) * 1989-08-31 1992-01-30
JP2507895B2 (en) * 1989-12-19 1996-06-19 工業技術院長 New synthesis system of ribozyme
WO1991019789A1 (en) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases
US6008343A (en) * 1990-06-19 1999-12-28 Gene Shears Pty. Ltd. Nucleotide based endonucleases
PL169576B1 (en) * 1990-10-12 1996-08-30 Max Planck Gesellschaft Method of obtaining rna molecule of catalytic activity
DE4104186A1 (en) * 1991-02-12 1992-08-13 Genentech Inc NEW COMPLEXES INCLUDING ENDOCYTOSIS IN HIGHER EUKARYOTIC CELLS, NUCLEIC ACID
FR2687411A1 (en) * 1992-02-13 1993-08-20 Nice Sophia Antipolis Universi Vector comprising a viral gene transcribed by RNA polymerase III, and process for the intercellular production of RNA
WO1993022433A2 (en) * 1992-04-28 1993-11-11 Frank Andreas Harald Meyer Medicament for the gene-therapeutic treatment of human beings, animals and plants, especially to block virus multiplication and carcinogenes and process for producing the medicament
US5989906A (en) * 1992-05-14 1999-11-23 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting P-glycoprotein (mdr-1-gene)
CA2135499A1 (en) * 1992-05-14 1993-11-25 James D. Thompson Method and reagent for inhibiting cancer development
US5610052A (en) * 1992-08-26 1997-03-11 Ribozyme Pharmaceuticals Inc. Enzymatic RNA with activity to ras
EP0596901B1 (en) * 1992-05-27 2000-08-09 City Of Hope CHIMERIC tRNA LYS -RIBOZYME MOLECULES
US5827935A (en) * 1992-05-27 1998-10-27 City Of Hope Chimeric tRNAlys -ribozyme molecules
WO1994000012A1 (en) * 1992-06-29 1994-01-06 Gene Shears Pty. Ltd. Nucleic acids and methods of use thereof for controlling viral pathogens
AU5968994A (en) * 1992-12-16 1994-07-04 Johanna E. Bergmann Agents for the prevention and treatment of human alzheimer's disease
DE69333216T2 (en) * 1993-07-06 2004-06-24 Université de Nice-Sophia Antipolis Vector, contains a virus gene transcribed in ARN polymerase III
US5837503A (en) * 1993-07-07 1998-11-17 Universite De Nice-Sophia-Antipolis Vector containing viral gene transcribed by RNA polymerase III and methods for use
US5817635A (en) * 1993-08-09 1998-10-06 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
WO1995019788A1 (en) * 1994-01-24 1995-07-27 City Of Hope Inhibitors and target molecule co-localization
DE4424761C1 (en) * 1994-07-04 1995-06-08 Max Planck Gesellschaft Expression cassette for continuous, stable prodn. of ribozyme
WO1996040906A1 (en) 1995-06-07 1996-12-19 Commonwealth Scientific And Industrial Research Organisation Optimized minizymes and miniribozymes and uses thereof
FR2755976B1 (en) * 1996-11-15 1999-01-15 Idm Immuno Designed Molecules NOVEL COMPLEXES OF NUCLEIC ACIDS AND POLYMER SUBSTITUTED BY RESIDUES CAUSING THE DESTABILIZATION OF CELL MEMBRANES
US6057156A (en) * 1997-01-31 2000-05-02 Robozyme Pharmaceuticals, Inc. Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
WO1999020753A2 (en) * 1997-10-21 1999-04-29 Ribozyme Pharmaceuticals, Inc. Peptide bond formation using nucleic acid catalysts
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
CZ295108B6 (en) 1998-03-20 2005-05-18 Benitec Australia Ltd Synthetic gene comprising dispersed or foreign deoxyribonucleic molecule and a gene construct containing such a synthetic gene
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
EP2314700A1 (en) 1999-01-28 2011-04-27 Medical College of Georgia Research Institute, Inc Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
CA2369944A1 (en) 2001-01-31 2002-07-31 Nucleonics Inc. Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods

Also Published As

Publication number Publication date
ATE140961T1 (en) 1996-08-15
FI901296A0 (en) 1990-03-15
ZA901973B (en) 1991-11-27
HU211868A9 (en) 1995-12-28
IE900931L (en) 1990-09-16
EP0387775B1 (en) 1996-07-31
CA2012312A1 (en) 1990-09-16
NO901213D0 (en) 1990-03-15
GR3020935T3 (en) 1996-12-31
NZ232917A (en) 1997-07-27
AU637354B2 (en) 1993-05-27
HU901584D0 (en) 1990-06-28
IL93754A (en) 1995-12-31
DE59010432D1 (en) 1996-09-05
PT93440B (en) 2001-07-31
ES2090049T3 (en) 1996-10-16
EP0387775A1 (en) 1990-09-19
KR900014591A (en) 1990-10-24
IE74850B1 (en) 1997-08-13
JPH03130080A (en) 1991-06-03
RU2136751C1 (en) 1999-09-10
HUT54206A (en) 1991-01-28
HU215187B (en) 1998-10-28
DD297838A5 (en) 1992-01-23
AU5130190A (en) 1990-11-01
FI105103B (en) 2000-06-15
NO901213L (en) 1990-09-17
JP3330930B2 (en) 2002-10-07
IL93754A0 (en) 1990-12-23
NO306623B1 (en) 1999-11-29
CA2012312C (en) 2001-08-14
PT93440A (en) 1990-11-07

Similar Documents

Publication Publication Date Title
DK0387775T3 (en) Genetic unit for inhibiting the function of RNA
ATE189264T1 (en) CHIMERIC OLIGONUCLEOTIDE AND USE THEREOF FOR PRODUCING NUCLEIC ACID TRANSCRIPTS
WO1996019568A3 (en) Stabilized ribozyme analogs
EP0572287A3 (en) Use of cyclic double-stranded oligonucleotides containing the binding site for a transcription factor for inhibiting gene expression
ES2180622T3 (en) OLIGONUCLEOTIDOS ANTISENTIDO WITH INHIBITING ACTIVITY OF TUMORIGENICITY.
ATE154029T1 (en) 7-DEAZAPURINE MODIFIED OLIGONUCLEOTIDES
DK0519053T3 (en) Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases
EP0708829B8 (en) A pharmaceutical composition comprising antisense-nucleic acid for prevention and/or treatment of neuronal injury, degeneration and cell death and for the treatment of neoplasms
HUP0103465A2 (en) Short oligonucleotides for the inhibition of vegf expression
Nakashima et al. Proximity of mRNA5′-region and 18S rRNA in eukaryotic initiation complexes
DK1175489T3 (en) Oligonucleotides containing an antisense sequence stabilized by a secondary structure and pharmaceutical preparations containing these oligonucleotides
DE69921609D1 (en) RIBOCYMAL NUCLEIC ACID CUTTING THE CCR5 OR CXCR4
Hatta et al. Mechanisms of the inhibition of reverse transcription by unmodified and modified antisense oligonucleotides
AU6144294A (en) Plant virus resistance conferring polyribozyme and resistant plants producing same
WO1995025801A3 (en) Viral replicon
AU3956100A (en) Rna polymerase i transcription factor tif-ia
Richardson et al. Identification of upstream sequence components of rho-dependent transcription terminators.
UA19318A (en) Method for human mno2-dismutase obtaining