DE865502T1 - FLUORESCENT-BASED DETECTION METHOD FOR LIGANDS - Google Patents

FLUORESCENT-BASED DETECTION METHOD FOR LIGANDS

Info

Publication number
DE865502T1
DE865502T1 DE0865502T DE96944304T DE865502T1 DE 865502 T1 DE865502 T1 DE 865502T1 DE 0865502 T DE0865502 T DE 0865502T DE 96944304 T DE96944304 T DE 96944304T DE 865502 T1 DE865502 T1 DE 865502T1
Authority
DE
Germany
Prior art keywords
ligands
fluorescent
detection method
based detection
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0865502T
Other languages
German (de)
Inventor
Robert T Sauer
Andrew Pakula
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scriptgen Pharmaceuticals Inc
Original Assignee
Scriptgen Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scriptgen Pharmaceuticals Inc filed Critical Scriptgen Pharmaceuticals Inc
Publication of DE865502T1 publication Critical patent/DE865502T1/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
DE0865502T 1995-12-07 1996-12-06 FLUORESCENT-BASED DETECTION METHOD FOR LIGANDS Pending DE865502T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US834695P 1995-12-07 1995-12-07
PCT/US1996/019698 WO1997020952A1 (en) 1995-12-07 1996-12-06 A fluorescence-based screening method for identifying ligands

Publications (1)

Publication Number Publication Date
DE865502T1 true DE865502T1 (en) 1999-08-19

Family

ID=21731102

Family Applications (1)

Application Number Title Priority Date Filing Date
DE0865502T Pending DE865502T1 (en) 1995-12-07 1996-12-06 FLUORESCENT-BASED DETECTION METHOD FOR LIGANDS

Country Status (5)

Country Link
EP (1) EP0865502A4 (en)
JP (1) JP2000502440A (en)
CA (1) CA2236339A1 (en)
DE (1) DE865502T1 (en)
WO (1) WO1997020952A1 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
CA2253587C (en) 1996-05-09 2008-01-29 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
US6571227B1 (en) 1996-11-04 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Method, system and computer program product for non-linear mapping of multi-dimensional data
US6453246B1 (en) 1996-11-04 2002-09-17 3-Dimensional Pharmaceuticals, Inc. System, method, and computer program product for representing proximity data in a multi-dimensional space
AU732397B2 (en) 1996-11-04 2001-04-26 3-Dimensional Pharmaceuticals, Inc. System, method and computer program product for identifying chemical compounds having desired properties
US6261842B1 (en) 1997-10-23 2001-07-17 Wisconsin Alumni Research Foundation Microorganism genomics, compositions and methods related thereto
JP2002514571A (en) * 1997-11-12 2002-05-21 ３−ディメンショナル ファーマシューティカルズ， インコーポレイテッド High-throughput method for functionally classifying proteins identified using genomic approaches
US20020035459A1 (en) 1998-09-14 2002-03-21 George M. Grass Pharmacokinetic-based drug design tool and method
US6569631B1 (en) 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
JP2002535989A (en) 1999-02-04 2002-10-29 アナディーズ・ファーマスーティカルズ，インコーポレイテッド Candida albicans SRB-7
US6972198B2 (en) 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using protein binding partners
US6376180B1 (en) * 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions
US6582907B1 (en) 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US7416524B1 (en) 2000-02-18 2008-08-26 Johnson & Johnson Pharmaceutical Research & Development, L.L.C. System, method and computer program product for fast and efficient searching of large chemical libraries
WO2001065462A2 (en) 2000-02-29 2001-09-07 3-Dimensional Pharmaceuticals, Inc. Method and computer program product for designing combinatorial arrays
AU2001247627A1 (en) 2000-03-22 2001-10-03 3-Dimensional Pharmaceuticals, Inc. System, method, and computer program product for representing object relationships in a multidimensional space
WO2001075790A2 (en) 2000-04-03 2001-10-11 3-Dimensional Pharmaceuticals, Inc. Method, system, and computer program product for representing object relationships in a multidimensional space
US7054757B2 (en) 2001-01-29 2006-05-30 Johnson & Johnson Pharmaceutical Research & Development, L.L.C. Method, system, and computer program product for analyzing combinatorial libraries
JP2004537047A (en) * 2001-06-14 2004-12-09 アナディーズ　ファーマスーティカルズ，アイエヌシー． Methods for screening ligands for target molecules
EP1637885A1 (en) * 2004-09-16 2006-03-22 Vivalis Method of screening by using conformation sensitive peptides
GB0421285D0 (en) 2004-09-24 2004-10-27 Univ Nottingham Improvements in high content screening
US8158340B2 (en) 2005-12-28 2012-04-17 Corning Incorporated Methods for detecting conformational changes in bioentities
EP3254113A4 (en) 2015-02-05 2018-06-27 Promega Corporation Luciferase-based thermal shift assays
GB202118173D0 (en) * 2021-12-15 2022-01-26 Medicines Discovery Catpult Ltd Protein stability assay

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5231755B2 (en) * 1973-01-08 1977-08-17
US5200504A (en) * 1990-10-02 1993-04-06 The Scripps Research Institute Metallopeptides having stabilized secondary structures
CA2115900A1 (en) * 1993-02-22 1994-08-23 Gerald W. Becker Pharmaceutical screens and antibodies

Also Published As

Publication number Publication date
EP0865502A1 (en) 1998-09-23
EP0865502A4 (en) 2000-08-16
JP2000502440A (en) 2000-02-29
WO1997020952A1 (en) 1997-06-12
CA2236339A1 (en) 1997-06-12

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