DE60017875D1 - Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen - Google Patents

Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen

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Publication number
DE60017875D1
DE60017875D1 DE60017875T DE60017875T DE60017875D1 DE 60017875 D1 DE60017875 D1 DE 60017875D1 DE 60017875 T DE60017875 T DE 60017875T DE 60017875 T DE60017875 T DE 60017875T DE 60017875 D1 DE60017875 D1 DE 60017875D1
Authority
DE
Germany
Prior art keywords
neural stem
culture
cell culture
proliferation
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60017875T
Other languages
English (en)
Other versions
DE60017875T2 (de
Inventor
Nobuko Uchida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
StemCells California Inc
Original Assignee
StemCells Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by StemCells Inc filed Critical StemCells Inc
Publication of DE60017875D1 publication Critical patent/DE60017875D1/de
Application granted granted Critical
Publication of DE60017875T2 publication Critical patent/DE60017875T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
DE60017875T 1999-02-26 2000-02-25 Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen Expired - Lifetime DE60017875T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US258529 1999-02-26
US09/258,529 US6238922B1 (en) 1999-02-26 1999-02-26 Use of collagenase in the preparation of neural stem cell cultures
PCT/US2000/005402 WO2000050572A1 (en) 1999-02-26 2000-02-25 Use of collagenase in the preparation of neural stem cell cultures

Publications (2)

Publication Number Publication Date
DE60017875D1 true DE60017875D1 (de) 2005-03-10
DE60017875T2 DE60017875T2 (de) 2006-04-06

Family

ID=22980962

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60017875T Expired - Lifetime DE60017875T2 (de) 1999-02-26 2000-02-25 Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen

Country Status (10)

Country Link
US (3) US6238922B1 (de)
EP (2) EP1155116B1 (de)
JP (2) JP4585124B2 (de)
AT (1) ATE288481T1 (de)
AU (1) AU764499B2 (de)
CA (1) CA2363851C (de)
DE (1) DE60017875T2 (de)
DK (1) DK1155116T3 (de)
ES (1) ES2235847T3 (de)
WO (1) WO2000050572A1 (de)

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US6238922B1 (en) * 1999-02-26 2001-05-29 Stemcells, Inc. Use of collagenase in the preparation of neural stem cell cultures
US20050042749A1 (en) * 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
US7250294B2 (en) * 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
JP5943533B2 (ja) 2000-05-17 2016-07-06 アステリアス バイオセラピューティクス インコーポレイテッド 神経前駆細胞の集団
US7838292B1 (en) 2001-03-29 2010-11-23 University Of Louisville Research Foundation, Inc. Methods for obtaining adult human olfactory progenitor cells
US7285415B2 (en) * 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050101014A1 (en) * 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
EP1578995A2 (de) * 2002-11-18 2005-09-28 Agency for Science, Technology and Research Verfahren und system zur isolierung vonzell- und/oder nukleinsäuremolekülen
US8617887B2 (en) * 2003-06-12 2013-12-31 Yeda Research And Development Co. Ltd Method of generating oligodendrocytes from neurosphere cells
CN1867678B (zh) * 2003-09-12 2012-10-03 干细胞技术公司 神经集落形成分析
US20070269412A1 (en) * 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
US7632681B2 (en) * 2003-12-02 2009-12-15 Celavie Biosciences, Llc Compositions and methods for propagation of neural progenitor cells
WO2005079250A2 (en) * 2004-02-13 2005-09-01 Cornell Research Foundation, Inc. Purines are self-renewal signals for neural stem cells, and purine receptor antagonists promote neuronal and glial differentiation therefrom
KR100563297B1 (ko) * 2004-06-16 2006-03-27 시 명 김 골프공 이송장치
US20080044890A1 (en) * 2004-06-30 2008-02-21 Parker Clayton R Interchangable sleeve for enhancing proliferation of cells in a rotating bioreactor
US7456019B2 (en) 2004-06-30 2008-11-25 Regenetech, Inc. Three-dimensional cell to tissue development process
US20060228795A1 (en) * 2004-06-30 2006-10-12 Parker Clayton R Apparatus for enhancing proliferation of cells in a small-scale cell culturing container
EP2317316A3 (de) * 2005-07-08 2011-06-15 Braincells, Inc. Verfahren zur Identifizierung von neurogenesemodulierenden Wirkstoffen und Bedingungen
CA2559513A1 (en) * 2005-09-12 2007-03-12 Stemcell Technologies Inc. Modified colony assay
WO2007130060A2 (en) * 2006-05-03 2007-11-15 Schepens Eye Research Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues
KR101335884B1 (ko) * 2006-07-24 2013-12-12 인터내셔날 스템 셀 코포레이션 망막 줄기 세포로부터 유래한 합성 각막
EP1900810A1 (de) * 2006-09-15 2008-03-19 Celogos Verfahren zur Zellextraktion und Zellselektion
US20100196328A1 (en) * 2007-04-25 2010-08-05 Tonya Bliss ISCHEMIA-INDUCED NEOVASCULARIZATION IS ENHANCED BY hCNS-SC TRANSPLANTATION
KR101635750B1 (ko) * 2008-01-30 2016-07-04 아스테리아스 바이오세라퓨틱스, 인크. 줄기 세포 유래 올리고덴드로사이트 전구 세포를 배양하기 위한 합성 표면
US8093053B2 (en) * 2008-03-17 2012-01-10 Uti Limited Partnership Methods and compositions for culturing of neural precursor cells
JP2011521639A (ja) * 2008-08-12 2011-07-28 インダストリー‐アカデミック・コオペレイション・ファウンデイション，ヨンセイ・ユニバーシティ ヒト神経幹細胞及びこれを利用した中枢または末梢神経系疾患及び損傷治療用薬学的組成物
US8309343B2 (en) * 2008-12-01 2012-11-13 Baxter International Inc. Apparatus and method for processing biological material
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
CA2844580A1 (en) * 2011-08-09 2013-02-14 Lpath, Inc. Stem cell therapy using inhibitors of lysophosphatidic acid
CN108449995B (zh) 2015-11-06 2022-02-01 文塔纳医疗系统公司 代表性诊断
CN109022360A (zh) * 2018-07-03 2018-12-18 湖南未名三胞转化医学科技有限公司 自体脂肪干细胞共培养促进外周血造血干细胞增殖方法

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US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
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US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
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WO1994009119A1 (en) 1992-10-16 1994-04-28 Neurospheres Ltd. Remyelination using neural stem cells
AU3338595A (en) 1994-07-29 1996-03-04 Dalhousie University Propagation and inducible differentiation of human fetal central nervous system progenitor cells
AU716811B2 (en) 1994-11-14 2000-03-09 Neurospheres Holdings Ltd Regulation of neural stem cell proliferation
CN1217746A (zh) * 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
US5753506A (en) * 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
US6238922B1 (en) * 1999-02-26 2001-05-29 Stemcells, Inc. Use of collagenase in the preparation of neural stem cell cultures

Also Published As

Publication number Publication date
JP4585124B2 (ja) 2010-11-24
EP1155116B1 (de) 2005-02-02
US7049141B1 (en) 2006-05-23
EP1155116A1 (de) 2001-11-21
WO2000050572A1 (en) 2000-08-31
ATE288481T1 (de) 2005-02-15
WO2000050572A9 (en) 2001-08-30
AU764499B2 (en) 2003-08-21
JP2010227127A (ja) 2010-10-14
US6238922B1 (en) 2001-05-29
DE60017875T2 (de) 2006-04-06
CA2363851C (en) 2011-01-25
ES2235847T3 (es) 2005-07-16
CA2363851A1 (en) 2000-08-31
US20010055808A1 (en) 2001-12-27
JP2002536991A (ja) 2002-11-05
EP1533367A1 (de) 2005-05-25
AU3716300A (en) 2000-09-14
DK1155116T3 (da) 2005-05-30

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: STEMCELLS CALIFORNIA, INC., PALO ALTO, CALIF., US