DE10343375A1 - Method for the detection and analysis of protein-protein interactions - Google Patents

Method for the detection and analysis of protein-protein interactions Download PDF

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Publication number
DE10343375A1
DE10343375A1 DE2003143375 DE10343375A DE10343375A1 DE 10343375 A1 DE10343375 A1 DE 10343375A1 DE 2003143375 DE2003143375 DE 2003143375 DE 10343375 A DE10343375 A DE 10343375A DE 10343375 A1 DE10343375 A1 DE 10343375A1
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DE
Germany
Prior art keywords
protein
detection
analysis
dollar
interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE2003143375
Other languages
German (de)
Inventor
Sylvia Gruenewald
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sygnis Pharma AG
Original Assignee
Axaron Bioscience AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axaron Bioscience AG filed Critical Axaron Bioscience AG
Priority to DE2003143375 priority Critical patent/DE10343375A1/en
Priority to PCT/EP2004/010391 priority patent/WO2005033706A1/en
Publication of DE10343375A1 publication Critical patent/DE10343375A1/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Detektion und Analyse von Protein-Interaktionen, umfassend die Verfahrensschritte DOLLAR A a) proteolytische Freisetzung mindestens eines Proteins mit mindestens einer detektierbaren Eigenschaft in Folge einer Protein-Interkation DOLLAR A b) Detektion und gegebenenfalls Quantifizierung der freigesetzten Proteine an Hand ihrer detektierbaren Eigenschaften. DOLLAR A Die Erfindung betrifft außerdem eine Ausführungsform des obigen Verfahrens, wobei Verfahrensschritt a) in vivo und Verfahrensschritt b) in vitro durchgeführt werden.The invention relates to a method for the detection and analysis of protein interactions comprising the method steps DOLLAR A a) proteolytic release of at least one protein with at least one detectable property as a result of a protein interaction DOLLAR A b) detection and optionally quantification of the released proteins on hand their detectable properties. DOLLAR A The invention also relates to an embodiment of the above method, wherein process step a) in vivo and process step b) are carried out in vitro.

DE2003143375 2003-09-17 2003-09-17 Method for the detection and analysis of protein-protein interactions Withdrawn DE10343375A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE2003143375 DE10343375A1 (en) 2003-09-17 2003-09-17 Method for the detection and analysis of protein-protein interactions
PCT/EP2004/010391 WO2005033706A1 (en) 2003-09-17 2004-09-16 Method for the detection and analysis of protein-protein interactions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2003143375 DE10343375A1 (en) 2003-09-17 2003-09-17 Method for the detection and analysis of protein-protein interactions

Publications (1)

Publication Number Publication Date
DE10343375A1 true DE10343375A1 (en) 2005-04-28

Family

ID=34398805

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2003143375 Withdrawn DE10343375A1 (en) 2003-09-17 2003-09-17 Method for the detection and analysis of protein-protein interactions

Country Status (2)

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DE (1) DE10343375A1 (en)
WO (1) WO2005033706A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102014203266A1 (en) 2014-02-24 2015-08-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Composition with FRET pair in defined geometry
DE102015006187A1 (en) * 2015-05-15 2016-11-17 Eberhard Karls Universität Tübingen Cloning system and method for the identification of interaction partners in protein complexes

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2374686T3 (en) 2007-05-14 2012-02-21 Historx, Inc. SEPARATION IN COMPARTMENTS BY CHARACTERIZATION OF PIXEL USING CLOTHING OF IMAGE DATA.
US7907271B2 (en) 2007-06-15 2011-03-15 Historx, Inc. Method and system for standardizing microscope instruments
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
CA2737116C (en) 2008-09-16 2019-01-15 Historx, Inc. Reproducible quantification of biomarker expression

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5585245A (en) * 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
US6333154B1 (en) * 1997-12-04 2001-12-25 Institut Pasteur Bacterial multi-hybrid system and applications thereof
AR020105A1 (en) * 1998-07-07 2002-04-10 Procter & Gamble FUSIONED PORTEASES WITH VARIANTE OF THE STREPTOMYCES SUBTILISIN INHIBITOR, DNA CODIFYING SUCH FUSED PROTEIN, COMPOSITION THAT INCLUDES LAMISM AND AN EXPRESSION SYSTEM THAT INCLUDES SUCH DNA
WO2001079559A1 (en) * 2000-04-18 2001-10-25 Wayne State University System to detect protein-protein interactions
AU2001261499A1 (en) * 2000-05-12 2001-11-26 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
EP1319954A1 (en) * 2001-12-12 2003-06-18 Centre National de Genotypage Methods for protein analysis using protein capture arrays
DE10211063A1 (en) * 2002-03-13 2003-10-09 Axaron Bioscience Ag New methods for the detection and analysis of protein interactions in vivo

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102014203266A1 (en) 2014-02-24 2015-08-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Composition with FRET pair in defined geometry
DE102014203266B4 (en) 2014-02-24 2018-07-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Composition with FRET pair in defined geometry
DE102015006187A1 (en) * 2015-05-15 2016-11-17 Eberhard Karls Universität Tübingen Cloning system and method for the identification of interaction partners in protein complexes
DE102015006187B4 (en) 2015-05-15 2018-12-13 Eberhard Karls Universität Tübingen Cloning system and method for the identification of interaction partners in protein complexes

Also Published As

Publication number Publication date
WO2005033706A1 (en) 2005-04-14

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Date Code Title Description
8127 New person/name/address of the applicant

Owner name: SYGNIS BIOSCIENCE GMBH & CO. KG, 69120 HEIDELB, DE

8141 Disposal/no request for examination