DE102008062772A1 - Disinfecting viruses on textiles and hard surfaces - Google Patents

Abstract

Textiles and / or hard surfaces can be disinfected by contacting them with a virucidal treatment solution comprising at least one hydrolytic enzyme.

Description

The Invention is directed to a method of disinfecting textiles and / or hard surfaces by a virucidal treatment solution, which comprises at least one hydrolytic enzyme.

at the laundry of textiles or cleaning hard surfaces such as bathroom tiles or Crockery is expected not only a visually perfect cleanliness, but more and more also an improved hygiene effect or disinfection the agent used for the cleaning. For disinfectants is a zufriendenstellende hygiene effect anyway necessary. Regarding the disinfection of textiles or hard surfaces, in particular by a washing, cleaning or disinfecting agent is to be achieved, is usually between the effectiveness against germs and effectiveness Viruses are different, whereby germs usually independently reproducible germs (so-called colony-forming units - CFU) are, in particular bacteria and fungi. The disinfection of Viruses on textiles or hard surfaces is in contrast so far unsatisfactory for the disinfection of germs.

Out The prior art discloses that washing or cleaning agents Contain enzymes that improve the cleaning performance of the agents. Typical enzymes in detergents or cleaners are proteases, amylases, Cellulases, lipases, but also other hydrolytic enzymes.

It It is also known that enzymes are capable of viruses inactivate. This applies in particular to RNases, DNases, Transcriptases or amino acid lyases. Some of these enzymes are hydrolytic enzymes such as RNases or DNases. The said antiviral enzymes are but mainly used in the therapy of viral infections. Furthermore, the Use of hydrolytic enzymes in virus analysis or in crop protection known. For example, proteases are used in the preparative Analysis of viral envelope proteins used. Neither RNases, DNases but still more hydrolytic enzymes are so far for the antiviral disinfection of textiles or hard surfaces intended.

For the inactivation of viruses on hard surfaces, the enzyme-based disinfectant Enzodine ^{TM is} known (cf. Kessler & Richards, Symbollon Corp., Sudbury, 1993 ) known. This comprises as enzyme a peroxidase which has a corresponding virucidal activity. However, this composition does not contain any hydrolytic enzyme, in particular no protease, amylase, cellulase, glycosidase, hemicellulase, mannanase, xylanase, pectinase, β-glucosidase, carrageenase or lipase.

Out the prior art is therefore not yet known that a hydrolytic enzyme has a virucidal activity, d. H. an effectiveness to a virus that possesses, if the hydrolytic Enzyme as part of a treatment solution with textiles or hard surfaces is brought into contact.

Surprisingly has now been found that textiles or hard surfaces Can be disinfected disinfected when using a virucidal solution be treated containing at least one hydrolytic enzyme. In particular, the virus activity is enhanced by the hydrolytic enzyme a solution containing this enzyme.

object The invention is thus a method for disinfecting textiles and / or hard surfaces, which is characterized is that the textile and / or the hard surface with a virucidal treatment solution containing at least one hydrolytic Enzyme is contacted. An inventive Therefore, the method causes at least a proportionate disinfection of the Textile or the hard surface.

Under Disinfection becomes effective in the context of the present invention towards at least one virus species (virus activity) Understood. A virucidal treatment solution is therefore one liquid composition that has viral efficacy has at least one virus species.

Under Viral efficacy will be any reduction of virus titer and thus connected to the infectiousness of a virus, said the infectivity is the ability of a virus to infect a host. Viral activity therefore becomes advantageous achieved by damage to the virus or viruses, especially with regard to adhesion to a host cell and / or to introduce the genetic material into a Host cell and / or for replication of the genetic material in a host cell. According to the invention, the hydrolytic enzyme, which is contained in the virucidal treatment solution, a Virus activity and therefore contributes to the virus effectiveness the virucidal treatment solution.

The determination of the viral efficacy of the hydrolytic enzyme as well as the virucidal treatment solution can be carried out according to the established and accepted methodology of the DVV / RKI guideline ( Guideline of the German Association for the Control of Viral Diseases e. V. and the Robert Koch Institute for the testing of chemical disinfectants for effectiveness against viruses in human medicine; Version of 15 June 2005, Bundesgesundheitsblatt (Bundesgesundheitsblatt 48, (2005) 1420-1426) to whose disclosure expressly referenced or their disclosure is expressly incorporated into the present application. This method is used to test chemical disinfectants for effectiveness against viruses, but it is also applicable if, for example, the virus effectiveness of a substance to be determined, for example, that of the contained in the virucidal treatment solution hydrolytic enzyme. The methodology is equally applicable to mixtures and preparations as described below. In this case, the substance mixture or the preparation is used instead of the hydrolytic enzyme.

It is an in vitro suspension test in which the decrease (reduction) of the virus titer in the presence of the hydrolytic enzyme as an active ingredient is determined in comparison with an active ingredient-free water solution and expressed as the logarithm of this reduction factor (RF). The virus titer is a measure of a concentration of a virus. It is determined by diluting the sample continuously and diluting it with a detection test for the particular virus to be determined by inoculating the dilutions on cell cultures whose cells are permissive to the virus in question. The most extensive dilution at which a positive test reaction is still detectable is given as titre. In the present case, therefore, a test batch with a hydrolytic enzyme is compared with a parallel batch without this hydrolytic enzyme. The hydrolytic enzyme is used in a concentration of 100 ppm (parts per million), 1 ppm = 10 ^-6 = 0.0001%). The exposure time in the context of the present invention is 15 or 30 minutes. RF values less than or equal to 1 are considered as non-significant viral efficacy in the context of the present invention. In contrast, RF values greater than one indicate viral activity, since in this case the decrease in the virus titer in the test batch is greater than the decrease in virus titer in the water batch. Viral activity is therefore already present when a reduction of the virus titer compared to the aqueous solution is present or detectable. It is not necessary that a complete virus activity, ie a complete inactivation of all viruses of a virus species, is present in the sense that no virus titer is no longer present or detectable. Viral activity is preferably always assumed if, after the tested exposure time under the respective test conditions, the virus titer is reduced by at least 50% and increasingly preferably by at least 60%, 70%, 80%, 90%, 95%, 96%, 97 %, 98%, 99% and most preferably 99.99% (equivalent to at least 4 log 10 steps). A preferred virus activity is indicated in particular by RF values of increasingly preferably 1.5, 2, 2.5, 3, 3.5 and particularly preferably 4 or more.

The mentioned DW / RKI guideline provides that the temperature during the exposure time is 20 ° C +/- 0.5 ° C. However, the guideline also explicitly allows lower ones Temperatures can be selected, provided one Inactivation of the viruses at lower temperatures provided is. Consequently, it is within the scope of determining viral efficacy in the context of the present application also possible, higher To choose temperatures during the exposure time, if provided, the hydrolytic enzyme or the containing it virucidal treatment solution at a higher temperature apply according to the invention.

Under Viruses (singular: virus) become intracellular, even though Non-cellular parasites understood the cells of living things can infect. Viruses contain the genetic program (Genetic material) in the form of at least one nucleic acid (Deoxyribonucleic acid (DNA) or ribonucleic acid (RNA)) and optionally also other auxiliary components for their multiplication and spread. However, they do not have their own metabolism, because they have no cytoplasm, which is a medium for metabolic processes and they lack ribosomes as well also mitochondria. Therefore, they alone can not produce proteins produce, do not convert energy and do not self-replicate. They are therefore dependent on the metabolism of the host cell. This makes them intracellular parasites. Essentially So a virus is a nucleic acid on which the information is are included for controlling the metabolism of a host cell, in particular for the replication of the virus nucleic acid and to further equip the virus particles (virions). A virus Therefore, in the sense of the present patent application, both a single virus or virus particle as well as a certain type of virus be, then of course several or many individual viruses or virus particles of the same type (for example, the same family, of the same genus, species or serotype) includes.

Viruses come in two forms. In the first appearance as nucleic acid in the cells of the host, this nucleic acid contains the information for its replication by the host cell and for the production of the second manifestation, the virus particle (virion). The virion is secreted to spread the virus from the host cells. Virions are about 15 to 400 nm sized particles consisting of nucleic acids and a protein shell (capsid). Virions are significantly smaller than bacteria. In addition, some virions are surrounded by a membrane called the virus envelope or have other additional constituents. For example, in some types of viruses, the virions have a lipoprotein envelope. Such viruses, which have a lipoprotein envelope in addition to the capsid temporarily until the beginning of the replication phase, are enveloped viruses, viruses without such a casing are non-enveloped viruses. Virions are suitable for spreading the viruses. They invade (infect) all or part (at least their nucleic acid) into the host cells, and the virus nucleic acid then programs their metabolism to increase the viral nucleic acid and to produce the other virion components.

A hydrolytic enzyme is a hydrolase (EC 3.XXX) and thus an enzyme that hydrolytically cleaves esters, ethers, peptides, glycosides, acid anhydrides or CC bonds in a reversible reaction. The hydrolytic enzyme therefore catalyzes the hydrolytic cleavage of substances according to AB + H ₂ O ↔ AH + B-OH.

hydrolases make up the third major class of EC classification of enzymes. The EC numbers (English Enzyme Commission numbers) form a numerical Classification system for enzymes. Each EC number exists of four numbers separated by dots, the first Numeral denotes one of the six major enzyme classes and hydrolases with E.C. 3.X.X.X according to represent the third main class. Their representatives are proteases, peptidases, nucleases, phosphatases, Glycosidases and esterases, wherein nucleases, in particular DNases and RNases, not as hydrolytic enzymes as defined herein Invention are to be considered.

Usually the viruses in the appearance of the virion lie on textiles and hard surfaces. According to the invention it is therefore possible in particular and advantageously, itself a virus that is in the form of a virion on the textile or the hard Surface is present with a hydrolytic enzyme too inactivate. Nucleases, especially DNases or RNases, are for this however unsuitable because they attack the genetic material of the virus, the in the Virion but not freely accessible, but in part extremely is packed with complex structures. Virions are on textiles or hard surfaces therefore for RNases or DNases not or with regard to an inventive Procedures only insufficiently addressable. In addition, such enzymes comparatively expensive. Overall, therefore, nucleases are not for suitable for comparatively large area applications, as they require methods according to the invention, and are therefore not as a hydrolytic enzyme in the sense of the present Considered invention.

According to the invention preferred Hydrolases are further preferred embodiments described below.

textiles include all types of tissue, even different Composition, for example, cotton, wool, silk, others Natural fibers, polyesters and blends of any kind. Preferred Textiles are laundry. Below this are the entirety the washable textiles understood. Too hard surfaces include, for example, surfaces of metal, Glass, porcelain, ceramics, tiles, stone, plastic, wood or painted surfaces. Too hard surfaces belong especially bathroom tiles, tiles, surfaces of Shower cubicles or other ceramic objects in sanitary Institutions. Too hard surfaces also count any kind of dishes, countertops, floor coverings or table surfaces. Also carpets and leather are hard according to the invention Surfaces attributed.

at a virucidal treatment solution may be, for example to an aqueous-based liquid, in particular Water to a mixture with one or more solvents, usually in liquid detergents or cleaners contained, or to a liquid on alcoholic Base, especially ethanol or isopropanol, act, with water is preferred. Such a liquid can be part of it include only the hydrolytic enzyme and then sets to ground the virus activity of the hydrolytic enzyme is an inventive virucidal treatment solution. The hydrolytic enzyme but can also be part of a liquid composition be, which in addition to the hydrolytic enzyme comprises other ingredients and a virucidal treatment solution according to the invention represents.

The concentration of the hydrolytic enzyme in the virucidal treatment solution is preferably in a range of 0.000005-0.1 g dry enzyme per 100 g treatment solution, and more preferably 0.0005-0.1 g dry enzyme per 100 g treatment solution and 0.005-0, 05 g dry enzyme per 100 g Treatment solution. In this regard, the weight of the virucidal treatment solution is determined so that the data are based on weight of enzyme and weight of virucidal treatment solution.

The concentration of the enzyme in the virucidal treatment solution can be determined by known methods, for example the BCA method (bicinchoninic acid, 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method ( Gornall AG, CS Bardawill and MM David, J. Biol. Chem., 177 (1948), pp. 751-766 ).

In Another embodiment of the invention is an inventive A method characterized in that the virucidal treatment solution further comprises at least one further disinfecting ingredient. Under a disinfecting ingredient are besides ingredients with a virus effectiveness also understood such ingredients, which additionally or alternatively have antimicrobial activity, So kill germs. The germicidal effect depends on the content of the disinfectant Ingredient in the virucidal treatment solution, wherein the germicidal effect with decreasing content of disinfecting Ingredient or increasing dilution of the virucidal Treatment solution decreases.

One the preferred disinfecting ingredient is ethanol or propanol. These monohydric alcohols are due to their solvent properties and their germicidal effects common in disinfectants and also used in detergents in general. It includes the Term "propanol" both the 1-propanol (n-propanol) as well as the 2-propanol ("isopropanol"). ethanol and / or propanol is, for example, in a total amount 10 to 65 wt .-%, preferably 25 to 55 wt .-% in the virucidal Treatment solution included. Another preferred disinfecting Ingredient is tea tree oil. This is the essential oil of the Australian Tea Tree (Melaleuca alternifolia), a native of New South Wales and Queensland evergreen shrub of the genus Myrtenheiden (Melaleuca), and other tea tree species from different genera (eg Baeckea, Kunzea and Leptospermum) in the family of myrtle plants (Myrtaceae). The tea tree oil is made by steam distillation from the leaves and branch tips of these trees won and is a mixture of approx. 100 substances; to the main ingredients include (+) - terpinene-4-ol, α-terpinene, terpinolene, Terpineol, pinene, myrcene, phellandrene, p-cymene, limonene and 1,8-cineole. Tea tree oil is, for example, in an amount of 0.05 to 10 wt .-%, preferably 0.1 to 5.0 wt .-%, in the virucidal treatment solution contain. Another preferred disinfecting ingredient is lactic acid. The lactic acid or 2-hydroxypropionic acid is a fermentation product made by different microorganisms is produced. She is weakly active in antibiotics. lactic acid is for example in amounts of up to 10 wt .-%, preferably 0.2 to 5.0 wt .-% contained in the virucidal treatment solution.

Further disinfecting ingredients are for example active ingredients the groups of alcohols, aldehydes, antimicrobial acids or their salts, carboxylic esters, acid amides, Phenols, phenol derivatives, diphenyls, diphenylalkanes, urea derivatives, Oxygen, nitrogen acetals and formals, benzamidines, isothiazoles and their derivatives, such as isothiazolines and isothiazolinones, phthalimide derivatives, Pyridine derivatives, antimicrobial surface-active compounds, Guanidines, antimicrobial amphoteric compounds, quinolines, 1,2-dibromo-2,4-dicyanobutane, iodo-2-propynyl-butyl-carbamate, iodine, Iodophores and peroxides. Among these, preferred active ingredients are preferred selected from the group comprising 1,3-butanediol, phenoxyethanol, 1,2-propylene glycol, glycerol, undecylenic acid, citric acid, Lactic acid, benzoic acid, salicylic acid, Thymol, 2-benzyl-4-chlorophenol, 2,2'-methylenebis (6-bromo-4-chlorophenol), 2,4,4'-trichloro-2'-hydroxydiphenyl ether, N- (4-chlorophenyl) -N- (3,4-dichlorophenyl) urea, N, N '- (1,10-decandiyldi-1-pyridinyl-4-ylidene) -bis- (1-octanamine) dihydrochloride, N, N'-bis (4-chlorophenyl) -3.12-diimino-2,4,11,13-tetraazatetradecanediimidamide, quaternary surface active compounds, guanidines. Preferred surface-active quaternary compounds contain an ammonium, sulfonium, phosphonium, iodonium or Arsonium. Furthermore, disinfectant essential oils can also be used used simultaneously for fragrancing the virucidal Provide treatment solution. Particularly preferred active ingredients are, however, selected from the group comprising salicylic acid, quaternary surfactants, in particular benzalkonium chloride, peroxo compounds, in particular Hydrogen peroxide, alkali metal hypochlorite and mixtures thereof. Such another disinfecting ingredient is, for example in an amount of 0.01 to 1% by weight, preferably 0.02 to 0.8 Wt .-%, in particular 0.05 to 0.5 wt .-%, particularly preferably 0.1 to 0.3% by weight, most preferably 0.2% by weight in contain the virucidal treatment solution.

In a further embodiment of the invention, the virucidal treatment solution further comprises a surfactant preparation. In the context of the present invention, the term "surfactant preparation" is understood to mean any composition which comprises at least one surfactant, preferably at least one of the following called surfactants. In a preferred embodiment, the surfactant preparation is in particular a diluted or undiluted washing, cleaning, textile pretreatment or aftertreatment agent or disinfectant. These are also understood below by the term "surfactant preparation". As a result, the treated textile or the treated hard surface undergoes not only a disinfection of viruses but also a cleaning of soiling and / or a disinfection of germs.

such Surfactant formulations may be used as such or after dilution with water in the context of the inventive method be used. Such surfactant preparations are for the cleaning and / or disinfection of textiles or hard surfaces, possibly with a soil deposited thereon, useful. If it is a liquid surfactant preparation it may be used as such according to the invention and provides a surfactant preparation according to the invention However, a corresponding preparation can also be diluted are then to represent the surfactant preparation according to the invention. Such a surfactant preparation can be readily prepared by: a measured amount of the surfactant preparation in a further amount of water is diluted in certain weight ratios of surfactant preparation: water and optionally this dilution is shaken to a uniform distribution to ensure the surfactant preparation in the water. Possible Weight or volume ratios of the dilutions are from 1: 0 surfactant preparation: water to 1: 10,000 or 1: 20000 surfactant preparation: water, preferably from 1:10 to 1: 2000 surfactant preparation: water. Further may also be an originally solid surfactant, so for example, a powdered preparation or a in tablet form, in a liquid and preferably dissolved in water, then a surfactant preparation represent in the context of the invention.

In this case, all solid, liquid or flowable, gelatinous, portion-packed or individually portionable, pulverulent, granulated, compressed into tablets, pasty, sprayable or in other conventional dosage forms ready-made means for mechanical or manual textile washing can serve as detergent. The detergents also include washing aids, which are added to the actual detergent in the manual or machine textile washing, in order to achieve a further effect. Detergents include all hard surface cleaning agents, including manual and machine dishwashing detergents, carpet cleaners, scouring agents, glass cleaners, toilet scavengers, etc., also found in all of these dosage forms. Textile pre- and post-treatment are finally on the one hand such means with which the laundry is brought into contact before the actual laundry, for example, for tapping stubborn dirt, on the other hand, those in a laundry downstream of the further step the laundry further desirable Give properties such as a comfortable grip, crease resistance or low static charge. Amongst others, the fabric softeners are calculated. Disinfectants are, for example, hand disinfectants, surface disinfectants and instrument disinfectants, which may also occur in all of the above dosage forms. A disinfectant preferably causes a germ reduction by a factor of at least 10 ⁴ , that is to say that of originally 10,000 proliferating germs (so-called colony-forming units - CFU) survived no more than a single, with viruses in this regard are not considered as germs, since they have no cytoplasm and have no own metabolism. Preferred disinfectants cause a germ reduction by a factor of at least 10 ⁵ . Also, disinfectants with an already existing germ-reducing effect can therefore be improved as part of a method according to the invention in their viral efficacy. "Flowable" in the sense of the present application are agents which are pourable and may have viscosities up to several tens of thousands of mPas. The viscosity can be measured by conventional standard methods (for example, Brookfield LVT-II viscosimeter at 20 rpm and 20 ° C., spindle 3) and is preferably in the range from 5 to 10,000 mPas. Preferred agents have viscosities of 10 to 8000 mPas, with values between 120 to 3000 mPas being particularly preferred.

When Surfactants are in particular anionic surfactants, nonionic surfactants and their mixtures, but also cationic, zwitterionic and amphoteric Surfactants in question.

suitable Nonionic surfactants are, in particular, alkyl glycosides and ethoxylation and / or propoxylation products of alkyl glycosides or linear or branched alcohols each having 12 to 18 carbon atoms in the alkyl moiety and 3 to 20, preferably 4 to 10 alkyl ether groups. Farther are corresponding ethoxylation and / or propoxylation products of N-alkylamines, vicinal diols, fatty acid esters and fatty acid amides, with respect to the alkyl part said long-chain alcohol derivatives correspond, and of alkylphenols having 5 to 12 carbon atoms in the alkyl radical useful.

As nonionic surfactants are preferably alkoxylated, advantageously ethoxylated, in particular dere primary alcohols having preferably 8 to 18 carbon atoms and an average of 1 to 12 moles of ethylene oxide (EO) per mole of alcohol used in which the alcohol radical may be linear or preferably methyl-branched in the 2-position or may contain linear and methyl-branched radicals in the mixture, as they are usually present in Oxoalkoholresten. In particular, however, alcohol ethoxylates with linear radicals of alcohols of native origin having 12 to 18 carbon atoms, for. From coconut, palm, tallow or oleyl alcohol, and on average from 2 to 8 EO per mole of alcohol. The preferred ethoxylated alcohols include, for example, C ₁₂ -C ₁₄ -alcohols with 3 EO or 4 EO, C ₉ -C ₁₁ -alcohols with 7 EO and 2-propylheptanol with 7 EO, C ₁₃ -C ₁₅ -alcohols with 3 EO, 5 EO, 7 EO or 8 EO, C ₁₂ -C ₁₈ -alcohols with 3 EO, 5 EO or 7 EO and mixtures of these, such as mixtures of C ₁₂ -C ₁₄ -alcohol with 3 EO and C ₁₂ -C ₁₈ - Alcohol with 7 EO. The degrees of ethoxylation given represent statistical means which, for a particular product, may be an integer or a fractional number. Preferred alcohol ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE). In addition to these nonionic surfactants, fatty alcohols with more than 12 EO can also be used. Examples include (tallow) fatty alcohols with 14 EO, 16 EO, 20 EO, 25 EO, 30 EO or 40 EO. Especially in surfactant formulations for use in mechanical processes usually extremely low-foam compounds are used. These include preferably C ₁₂ -C ₁₈ -alkylpolyethylenglykol-polypropylene glycol ethers with in each case at to 8 mol ethylene oxide and propylene oxide units in the molecule. One can also use other known low-foaming nonionic surfactants such as C ₁₂ -C _{1 8} alkyl polyethylene glycol polybutylene each having up to 8 moles of ethylene oxide and butylene oxide units in the molecule and end-capped Alkylpolyalkylenglykolmischether. Also particularly preferred are the hydroxyl-containing alkoxylated alcohols, as described in the European patent application EP 0 300 305 are described, so-called Hydroxymischether. The nonionic surfactants also include alkyl glycosides of the general formula RO (G) _x in which R is a primary straight-chain or methyl-branched, in particular 2-methyl-branched aliphatic radical having 8 to 22, preferably 12 to 18 carbon atoms and G represents a glycose unit having 5 or 6 C atoms, preferably glucose. The degree of oligomerization x, which indicates the distribution of monoglycosides and oligoglycosides, is an arbitrary number - which, as a variable to be determined analytically, may also assume fractional values - between 1 and 10; preferably x is 1.2 to 1.4. Also suitable are polyhydroxy fatty acid amides of the formula (III) in which R ¹ CO is an aliphatic acyl radical having 6 to 22 carbon atoms, R ^{2 is} hydrogen, an alkyl or hydroxyalkyl radical having 1 to 4 carbon atoms and [Z] is a linear or branched polyhydroxyalkyl radical having 3 to 10 carbon atoms and 3 to 10 hydroxyl groups:

in der R³ für einen linearen oder verzweigten Alkyl- oder Alkenylrest mit 7 bis 12 Kohlenstoffatomen, R⁴ für einen linearen, verzweigten oder cyclischen Alkylenrest oder einen Arylenrest mit 2 bis 8 Kohlenstoffatomen und R⁵ für einen linearen, verzweigten oder cyclischen Alkylrest oder einen Arylrest oder einen Oxy-Alkylrest mit 1 bis 8 Kohlenstoffatomen steht, wobei C₁-C₄-Alkyl- oder Phenylreste bevorzugt sind, und [Z] für einen linearen Polyhydroxyalkylrest, dessen Alkylkette mit mindestens zwei Hydroxylgruppen substituiert ist, oder alkoxylierte, vorzugsweise ethoxylierte oder propoxylierte Derivate dieses Restes steht. [Z] wird auch hier vorzugsweise durch reduktive Aminierung eines Zuckers wie Glucose, Fructose, Maltose, Lactose, Galactose, Mannose oder Xylose erhalten. Die N-Alkoxy- oder N-Aryloxy-substituierten Verbindungen können dann beispielsweise durch Umsetzung mit Fettsäuremethylestern in Gegenwart eines Alkoxids als Katalysator in die gewünschten Polyhydroxyfettsäureamide überführt werden. Eine weitere Klasse bevorzugt eingesetzter nichtionischer Tenside, die entweder als alleiniges nichtionisches Tensid oder in Kombination mit anderen nichtionischen Tensiden, insbesondere zusammen mit alkoxylierten Fettalkoholen und/oder Alkylglykosiden, eingesetzt werden, sind alkoxylierte, vorzugsweise ethoxylierte oder ethoxylierte und propoxylierte Fettsäurealkylester, vorzugsweise mit 1 bis 4 Kohlenstoffatomen in der Alkylkette, insbesondere Fettsäuremethylester. Auch nichtionische Tenside vom Typ der Aminoxide, beispielsweise N-Kokosalkyl-N,N-dimethylaminoxid und N-Talgalkyl-N,N-dihydroxyethylaminoxid, und der Fettsäurealkanolamide können geeignet sein. Die Menge dieser nichtionischen Tenside beträgt vorzugsweise nicht mehr als die der ethoxylierten Fettalkohole, insbesondere nicht mehr als die Hälfte davon. Als weitere Tenside kommen sogenannte Gemini-Tenside in Betracht. Hierunter werden im Allgemeinen solche Verbindungen verstanden, die zwei hydrophile Gruppen pro Molekül besitzen. Diese Gruppen sind in der Regel durch einen sogenannten ”Spacer” voneinander getrennt. Dieser Spacer ist in der Regel eine Kohlenstoffkette, die lang genug sein sollte, dass die hydrophilen Gruppen einen ausreichenden Abstand haben, damit sie unabhängig voneinander agieren können. Derartige Tenside zeichnen sich im Allgemeinen durch eine ungewöhnlich geringe kritische Micellkonzentration und die Fähigkeit, die Oberflächenspannung des Wassers stark zu reduzieren, aus. In Ausnahmefällen werden unter dem Ausdruck Gemini-Tenside nicht nur derartig ”dimere”, sondern auch entsprechend ”trimere” Tenside verstanden. Geeignete Gemini-Tenside sind beispielsweise sulfatierte Hydroxymischether oder Dimeralkohol-bis- und Trimeralkohol-tris-sulfate und -ethersulfate. Endgruppenverschlossene dimere und trimere Mischether zeichnen sich insbesondere durch ihre Bi- und Multifunktionalität aus. So besitzen die genannten endgruppenverschlossenen Tenside gute Netzeigenschaften und sind dabei schaumarm, so dass sie sich insbesondere für den Einsatz in maschinellen Wasch- oder Reinigungsverfahren eignen. Eingesetzt werden können aber auch Gemini-Polyhydroxyfettsäureamide oder Poly-Polyhydroxyfettsäureamide. Geeignet sind auch die Schwefelsäuremonoester der mit 1 bis 6 Mol Ethylenoxid ethoxylierten geradkettigen oder verzweigten C₇-C₂₁-Alkohole, wie 2-Methylverzweigte C₉-C₁₁-Alkohole mit im Durchschnitt 3,5 Mol Ethylenoxid (EO) oder C₁₂-C₁₈-Fettalkohole mit 1 bis 4 EO. Zu den bevorzugten Aniontensiden gehören auch die Salze der Alkylsulfobernsteinsäure, die auch als Sulfosuccinate oder als Sulfobernsteinsäureester bezeichnet werden, und die Monoester und/oder Diester der Sulfobernsteinsäure mit Alkoholen, vorzugsweise Fettalkoholen und insbesondere ethoxylierten Fettalkoholen darstellen. Bevorzugte Sulfosuccinate enthalten C₈- bis C₁₈-Fettalkoholreste oder Mischungen aus diesen. Insbesondere bevorzugte Sulfosuccinate enthalten einen Fettalkoholrest, der sich von ethoxylierten Fettalkoholen ableitet, die für sich betrachtet nichtionische Tenside darstellen. Dabei sind wiederum Sulfosuccinate, deren Fettalkohol-Reste sich von ethoxylierten Fettalkoholen mit eingeengter Homologenverteilung ableiten, besonders bevorzugt. Ebenso ist es auch möglich, Alk(en)ylbernsteinsäure mit vorzugsweise 8 bis 18 Kohlenstoffatomen in der Alk(en)ylkette oder deren Salze einzusetzen. Als weitere anionische Tenside kommen Fettsäure-Derivate von Aminosäuren, beispielsweise von N-Methyltaurin (Tauride) und/oder von N-Methylglycin (Sarkoside) in Betracht. Insbesondere bevorzugt sind dabei die Sarkoside beziehungsweise die Sarkosinate und hier vor allem Sarkosinate von höheren und gegebenenfalls einfach oder mehrfach ungesättigten Fettsäuren wie Oleylsarkosinat. Als weitere anionische Tenside kommen insbesondere Seifen in Betracht. Geeignet sind insbesondere gesättigte Fettsäureseifen, wie die Salze der Laurinsäure, Myristinsäure, Palmitinsäure, Stearinsäure, hydrierten Erucasäure und Behensäure sowie insbesondere aus natürlichen Fettsäuren, zum Beispiel Kokos-, Palmkern- oder Talgfettsäuren, abgeleitete Seifengemische. Zusammen mit diesen Seifen oder als Ersatzmittel für Seifen können auch die bekannten Alkenylbernsteinsäuresalze eingesetzt werden.The polyhydroxy fatty acid amides are preferably derived from reducing sugars having 5 or 6 carbon atoms, in particular from glucose. The group of polyhydroxy fatty acid amides also includes compounds of the formula (IV)

in the R ^{3 is} a linear or branched alkyl or alkenyl radical having 7 to 12 carbon atoms, R ^{4 is} a linear, branched or cyclic alkylene radical or an arylene radical having 2 to 8 carbon atoms and R ^{5 is} a linear, branched or cyclic alkyl radical or a Aryl radical or an oxy-alkyl radical having 1 to 8 carbon atoms, wherein C ₁ -C ₄ alkyl or phenyl radicals are preferred, and [Z] is a linear polyhydroxyalkyl radical whose alkyl chain is substituted with at least two hydroxyl groups, or alkoxylated, preferably ethoxylated or propoxylated derivatives of this group. [Z] is also obtained here preferably by reductive amination of a sugar such as glucose, fructose, maltose, lactose, galactose, mannose or xylose. The N-alkoxy- or N-aryloxy-substituted compounds can then be converted into the desired polyhydroxy fatty acid amides, for example by reaction with fatty acid methyl esters in the presence of an alkoxide as catalyst. Another class of preferred nonionic surfactants used either as the sole nonionic surfactant or in combination with other nonionic surfactants, in particular together with alkoxylated fatty alcohols and / or alkyl glycosides, are alkoxylated, preferably ethoxylated or ethoxylated and propoxylated fatty acid alkyl esters, preferably from 1 to 4 carbon atoms in the alkyl chain, especially fatty acid methyl ester. Nonionic surfactants of the amine oxide type, for example N-cocoalkyl-N, N-dimethylamine oxide and N-tallowalkyl-N, N-dihydroxyethylamine oxide, and the fatty acid alkanolamides may also be suitable. The amount of these nonionic surfactants is preferably not more than that of the ethoxylated fatty alcohols, especially not more than that Half of it. Other suitable surfactants are so-called gemini surfactants. These are generally understood as meaning those compounds which have two hydrophilic groups per molecule. These groups are usually separated by a so-called "spacer". This spacer is typically a carbon chain that should be long enough for the hydrophilic groups to be spaced sufficiently apart for them to act independently of each other. Such surfactants are generally characterized by an unusually low critical micelle concentration and the ability to greatly reduce the surface tension of the water. In exceptional cases, the term gemini surfactants not only such "dimer", but also corresponding to "trimeric" surfactants understood. Suitable gemini surfactants are, for example, sulfated hydroxy mixed ethers or dimer alcohol bis and trimer alcohol tris sulfates and ether sulfates. End-capped dimeric and trimeric mixed ethers are characterized in particular by their bi- and multi-functionality. Thus, the end-capped surfactants mentioned have good wetting properties and are low foaming, so that they are particularly suitable for use in machine washing or cleaning processes. However, it is also possible to use gemini-polyhydroxy fatty acid amides or poly-polyhydroxy fatty acid amides. Also suitable are the sulfuric acid monoesters of straight-chain or branched C ₇ -C ₂₁ -alcohols ethoxylated with from 1 to 6 mol of ethylene oxide, such as 2-methyl-branched C ₉ -C ₁₁ -alcohols having on average 3.5 mol of ethylene oxide (EO) or C ₁₂ - C ₁₈ -fatty alcohols with 1 to 4 EO. The preferred anionic surfactants also include the salts of alkylsulfosuccinic acid, which are also referred to as sulfosuccinates or as sulfosuccinic acid esters, and the monoesters and / or diesters of sulfosuccinic acid with alcohols, preferably fatty alcohols and in particular ethoxylated fatty alcohols. Preferred sulfosuccinates contain C ₈ - C ₁₈ fatty alcohol radicals or mixtures thereof. Particularly preferred sulfosuccinates contain a fatty alcohol residue derived from ethoxylated fatty alcohols, which by themselves are nonionic surfactants. Sulfosuccinates, whose fatty alcohol residues are derived from ethoxylated fatty alcohols with a narrow homolog distribution, are again particularly preferred. Likewise, it is also possible to use alk (en) ylsuccinic acid having preferably 8 to 18 carbon atoms in the alk (en) yl chain or salts thereof. Suitable further anionic surfactants are fatty acid derivatives of amino acids, for example N-methyltaurine (Tauride) and / or N-methylglycine (sarcosides). Particularly preferred are the sarcosides or the sarcosinates and here especially sarcosinates of higher and optionally monounsaturated or polyunsaturated fatty acids such as oleyl sarcosinate. As further anionic surfactants are particularly soaps into consideration. Particularly suitable are saturated fatty acid soaps, such as the salts of lauric acid, myristic acid, palmitic acid, stearic acid, hydrogenated erucic acid and behenic acid and, in particular, soap mixtures derived from natural fatty acids, for example coconut, palm kernel or tallow fatty acids. Together with these soaps or as a substitute for soaps, it is also possible to use the known alkenylsuccinic acid salts.

The anionic surfactants, including soaps in the form of their sodium, potassium or ammonium salts and as soluble Salts of organic bases, such as mono-, di- or triethanolamine, are present. The anionic surfactants are preferably in the form of their sodium or potassium salt, especially in the form of the sodium salts.

surfactants are in the surfactant preparation, preferably in proportions of From 5% by weight to 50% by weight, in particular from 8% by weight to 30% by weight, contain.

In Another preferred embodiment comprises Surfactant preparation at least one further ingredient, preferably one selected from the group consisting of Builder, peroxygen compound, bleach activator, alcohol, acid, Grayness inhibitor, optical brightener, foam inhibitor, water-soluble Salt, polymeric thickener, volatile alkali and / or Base, hydrophilizing agent and combinations thereof.

Especially a combination of the hydrolytic enzyme as a first component with one or more other constituents of the virucidal Treatment solution as a second component proves to be advantageous because such a treatment solution further improved Viral effectiveness caused by resulting synergies. This means, that the treatment solution has an improved viral efficacy compared with a treatment solution, the either contains only one of the two components, or also in the Comparison to the expected virus effectiveness of a treatment solution with both components due to the mere addition of the respective individual contributions of these two components to Virus effectiveness. In particular, by the combination of the hydrolytic Enzyme with one of the other disinfecting described above Ingredients and / or with one of the surfactants described above and / or with one of the builders described below and / or with one of the peroxygen compounds described below and / or with one of the alcohols described below is a achieved such synergy.

A surfactant preparation according to the invention may further contain at least one water-soluble and / or water-insoluble, organic and / or inorganic builder. The water-soluble organic builder substances include polycarboxylic acids, in particular citric acid and sugar acids, monomeric and polymeric aminopolycarboxylic acids, in particular methylglycinediacetic acid, nitrilotriacetic acid and ethylenediaminetetraacetic acid and polyaspartic acid, polyphosphonic acids, in particular aminotris (methylenephosphonic acid), ethylenediaminetetrakis (methylenephosphonic acid) and 1-hydroxyethane-1,1-diphosphonic acid, polymeric hydroxy compounds such as dextrin and also polymeric (poly) carboxylic acids, in particular the polycarboxylates obtainable by oxidation of polysaccharides or dextrins, polymeric acrylic acids, methacrylic acids, maleic acids and copolymers thereof, which may also contain polymerized small amounts of polymerizable substances without carboxylic acid functionality. The molecular weight of the homopolymers of unsaturated carboxylic acids is generally between 3,000 and 200,000, of the copolymers between 2,000 and 200,000, preferably 30,000 to 120,000, each based on the free acid. A particularly preferred acrylic acid-maleic acid copolymer has a molecular weight of from 30,000 to 100,000. Commercial products are, for example Sokalan ^® CP 5, CP 10 and PA 30 from BASF. Suitable, although less preferred, compounds of this class are copolymers of acrylic or methacrylic acid with vinyl ethers, such as vinylmethyl ethers, vinyl esters, ethylene, propylene and styrene, in which the acid content is at least 50% by weight. It is also possible to use terpolymers which contain two unsaturated acids and / or salts thereof as monomers and also vinyl alcohol and / or an esterified vinyl alcohol or a carbohydrate as the third monomer as water-soluble organic builder substances. The first acidic monomer or its salt is derived from a monoethylenically unsaturated C ₃ -C ₈ -carboxylic acid and preferably from a C ₃ -C ₄ -monocarboxylic acid, in particular from (meth) -acrylic acid. The second acidic monomer or its salt may be a derivative of a C ₄ -C ₈ -dicarboxylic acid, with maleic acid being particularly preferred, and / or a derivative of an allylsulfonic acid which is substituted in the 2-position by an alkyl or aryl radical. Such polymers generally have a molecular weight between 1,000 and 200,000. Further preferred copolymers are those which preferably have as monomers acrolein and acrylic acid / acrylic acid salts or vinyl acetate. The organic builder substances can be used, in particular for the preparation of liquid surfactant preparations, in the form of aqueous solutions, preferably in the form of 30 to 50 percent by weight aqueous solutions. All of the acids mentioned are generally used in the form of their water-soluble salts, in particular their alkali metal salts.

such If desired, organic builders may be used in amounts up to 40 wt .-%, in particular up to 25 wt .-% and preferably from 1% by weight to 8% by weight. Quantities near the mentioned Upper limit are preferably in pasty or liquid, especially hydrous, surfactant used.

Suitable water-soluble inorganic builder materials are, in particular, alkali metal silicates, alkali metal carbonates and alkali metal phosphates, which may be in the form of their alkaline, neutral or acidic sodium or potassium salts. Examples of these are trisodium phosphate, tetrasodium diphosphate, disodium dihydrogen diphosphate, pentasodium triphosphate, so-called sodium hexametaphosphate, oligomeric trisodium phosphate with degrees of oligomerization of from 5 to 1000, in particular from 5 to 50, and the corresponding potassium salts or mixtures of sodium and potassium salts. As water-insoluble, water-dispersible inorganic builder materials are in particular crystalline or amorphous alkali metal aluminosilicates, in amounts of up to 50 wt .-%, preferably not more than 40 wt .-% and in liquid surfactant preparations, in particular from 1 wt .-% to 5 wt .-%, used. Among these, the crystalline sodium aluminosilicates in detergent quality, more particularly zeolite A, P and optionally X, alone or in mixtures, for example in the form of a co-crystals of zeolites A and X (VEGOBOND ^® AX, a commercial product of Condea August S. p. A.), preferred. Amounts near the stated upper limit are preferably used in solid, particulate surfactant formulations. In particular, suitable aluminosilicates have no particles with a particle size greater than 30 .mu.m and preferably consist of at least 80% by weight of particles having a size of less than 10 .mu.m. Their calcium binding capacity, according to the information of the German Patent DE 24 12 837 can be determined, is usually in the range of 100 to 200 mg CaO per gram.

Suitable substitutes or partial substitutes for the said aluminosilicate are crystalline alkali silicates which may be present alone or in a mixture with amorphous silicates. The alkali metal silicates useful as builders in the surfactant formulations preferably have a molar ratio of alkali metal oxide to SiO ₂ below 0.95, in particular from 1: 1.1 to 1:12, and may be present in amorphous or crystalline form. Preferred alkali metal silicates are the sodium silicates, in particular the amorphous sodium silicates, with a molar ratio of Na ₂ O: SiO ₂ of 1: 2 to 1: 2.8. Crystalline silicates which may be present alone or in a mixture with amorphous silicates are preferably crystalline sheet silicates of the general formula Na ₂ Si _x O ₂ × _{+ 1} × y H ₂ O. in which x, the so-called modulus, is a number from 1.9 to 22, in particular 1.9 to 4 and y is a number from 0 to 33 and preferred values for x are 2, 3 or 4. Preferred crystalline phyllosilicates are those in which x in the abovementioned general formula assumes the values 2 or 3. In particular, both β- and δ-sodium disilicates (Na ₂ Si ₂ O ₅ .yH ₂ O) are preferred. Also prepared from amorphous alkali metal silicates, practically anhydrous crystalline alkali silicates of the abovementioned general formula in which x is a number from 1.9 to 2.1, can be used in surfactant preparations according to the invention. In a further preferred surfactant preparation, a crystalline sodium layer silicate having a modulus of 2 to 3 is used, as can be prepared from sand and soda. Crystalline sodium silicates with a modulus in the range of 1.9 to 3.5 are used in a further preferred surfactant preparation. Crystalline layer-form silicates of formula (I) given above are sold by Clariant GmbH under the trade name Na-SKS, eg. Na-SKS-1 (Na ₂ Si ₂₂ O ₄₅ .xH ₂ O, Kenyaite), Na-SKS-2 (Na ₂ Si ₁₄ O ₂₉ .xH ₂ O, magadiite), Na-SKS-3 (Na ₂ Si ₈ O ₁₇ .xH ₂ O) or Na-SKS-4 (Na ₂ Si ₄ O ₉ .xH ₂ O, Makatite). Of these, especially Na-SKS-5 (α-Na ₂ Si ₂ O ₅ ), Na-SKS-7 (β-Na ₂ Si ₂ O ₅ , natrosilite), Na-SKS-9 (NaHSi ₂ O ₅ · 3H ₂ O), Na-SKS-10 (NaHSi ₂ O ₅ · 3H ₂ O, kanemite), Na-SKS-11 (t-Na ₂ Si ₂ O ₅₎ and Na-SKS-13 (NaHSi ₂ O ₅ ), but especially Na-SKS-6 (δ-Na ₂ Si ₂ O ₅ ). In a preferred embodiment a surfactant formulation in accordance with the invention utilizes a granular compound of crystalline layered silicate and citrate, of crystalline layered silicate and the above-mentioned (co) polymeric polycarboxylic acid or alkali metal silicate and alkali metal carbonate, for example, under the name Nabion ^® as 15 is commercially available.

builders are preferably in amounts of up to 75 in the surfactant formulations Wt .-%, in particular 5 wt .-% to 50 included.

When for use in surfactant preparations in the context of the invention suitable peroxygen compounds are in particular organic Peracids or peracid salts of organic acids, such as phthalimidopercaproic acid, perbenzoic acid or salts of diperdodecanedioic acid, hydrogen peroxide and under the washing conditions hydrogen peroxide-releasing inorganic Salts, which include perborate, percarbonate, persilicate and / or persulfate like Caroat belong. If solid peroxygen compounds can be used, they can be in the form of powders or granules are used, which are also known in principle Wrapped up. If a surfactant preparation Contains peroxygen compounds, these are in quantities of preferably up to 50% by weight, in particular from 5% by weight to 30 wt .-%, present. The addition of small amounts of known bleach stabilizers such as phosphonates, bristles or metaborates and metasilicates as well as magnesium acids such as magnesium sulfate be expedient.

When Bleach activators may be compounds that are under perhydrolysis conditions aliphatic peroxycarboxylic acids with preferably 1 to 10 C-atoms, in particular 2 to 4 C-atoms, and / or optionally substituted Perbenzoic acid, are used. Are suitable Substances which contain O- and / or N-acyl groups of said C atomic number and / or optionally substituted benzoyl groups. Preferred are multiply acylated alkylenediamines, in particular tetraacetylethylenediamine (TAED), acylated triazine derivatives, in particular 1,5-diacetyl-2,4-dioxohexahydro-1,3,5-triazine (DADHT), acylated glycolurils, especially tetraacetylglycoluril (TAGU), N-acylimides, especially N-nonanoylsuccinimide (NOSI), acylated Phenolsulfonates, especially n-nonanoyl or isononanoyloxybenzenesulfonate (n- or iso-NOBS), carboxylic anhydrides, in particular Phthalic anhydride, acylated polyhydric alcohols, in particular Triacetin, ethylene glycol diacetate, 2,5-diacetoxy-2,5-dihydrofuran and enol ester, as well as acetylated sorbitol and mannitol, respectively their described mixtures (SORMAN), acylated sugar derivatives, in particular pentaacetylglucose (PAG), pentaacetyl-fructose, tetraacetylxylose and octaacetyl lactose and acetylated, optionally N-alkylated Glucamine and gluconolactone, and / or N-acylated lactams, for example N-benzoyl. The hydrophilic substituted acylacetals and The acyl lactams are also preferably used. Also combinations Conventional bleach activators can be used. Such bleach activators can, especially in the presence above-mentioned hydrogen peroxide-supplying bleach, in the usual Amount range, preferably in amounts of 0.5 wt .-% to 10 wt .-%, in particular 1% by weight to 8% by weight, based on the total surfactant preparation, be missing when using percarboxylic acid as sole bleach, however, preferably completely.

additionally to the conventional bleach activators or in their place also sulfonimines and / or bleach-enhancing transition metal salts or transition metal complexes as so-called bleach catalysts be included.

Among the surfactant preparations, especially when they are in liquid or pasty form, usable in addition to water, organic solvents include alcohols having 1 to 4 carbon atoms, in particular methanol, ethanol, isopropanol and tert-butanol, diols having 2 to 4 carbon atoms, in particular ethylene glycol and propylene glycol, and mixtures thereof and the derivable from said compound classes ethers. Such water-miscible solvents are preferably present in the surfactant formulations in amounts not exceeding 30% by weight, in particular from 6% by weight to 20% by weight.

to Setting a desired, through the mixture the remaining components are not self-generating pH the surfactant preparations can be system- and environmentally compatible Acids, in particular citric acid, acetic acid, Tartaric acid, malic acid, lactic acid, glycolic acid, Succinic acid, glutaric acid and / or adipic acid, but also mineral acids, in particular sulfuric acid, or bases, in particular ammonium or alkali metal hydroxides. Such pH regulators are present in the surfactant preparations in amounts preferably not more than 20% by weight, in particular of 1.2 wt .-% to 17 wt .-%, contained.

graying have the task of removing the dirt from the textile fiber to keep suspended in the fleet. These are water-soluble Colloids mostly organic nature suitable, such as starch, Glue, gelatin, salts of ether carboxylic acids or ether sulfonic acids starch or cellulose or salts of acidic sulfuric acid esters cellulose or starch. Also water-soluble, Acidic group-containing polyamides are for this purpose suitable. Furthermore, other than the above-mentioned starch derivatives can be used use, for example, aldehyde levels. To be favoured Cellulose ethers, such as carboxymethyl cellulose (Na salt), methyl cellulose, Hydroxyalkylcellulose and mixed ethers, such as methylhydroxyethylcellulose, Methylhydroxypropylcellulose, methylcarboxymethylcellulose and their Mixtures, for example in amounts of 0.1 to 5 wt .-%, based on the surfactant preparation used.

laundry detergent As optical brighteners, for example, derivatives of Diaminostilbendisulfonsäure or their alkali metal salts although they are preferable for use as a color detergent free from optical brighteners. For example, salts are suitable 4,4'-bis (2-anilino-4-morpholino-1,3,5-triazinyl-6-amino) stilbene-2,2'-disulfonic acid or similar compounds, instead of the morpholino group a diethanolamino group, a methylamino group, an anilino group or carry a 2-methoxyethylamino group. Furthermore you can Brighteners of the type of substituted diphenylstyrene may be present for example, the alkali salts of 4,4'-bis (2-sulfostyryl) diphenyl, 4,4'-bis (4-chloro-3-sulfostyryl) -diphenyl, or 4- (4-chlorostyryl) -4 '- (2-sulfostyryl) -diphenyls. Also mixtures The aforementioned optical brightener can be used.

In particular when used in mechanical processes, it may be advantageous to add conventional foam inhibitors to the surfactant preparations. As foam inhibitors are, for example, soaps of natural or synthetic origin, which have a high proportion of C ₁₈ -C ₂₄ fatty acids. Suitable non-surfactant foam inhibitors are, for example, organopolysiloxanes and mixtures thereof with microfine, optionally silanized silica and paraffins, waxes, microcrystalline waxes and mixtures thereof with silanated silicic acid or bis-fatty acid alkylenediamides. It is also advantageous to use mixtures of various foam inhibitors, for example those of silicones, paraffins or waxes. The foam inhibitors, in particular silicone- and / or paraffin-containing foam inhibitors, are preferably bound to a granular, water-soluble or dispersible carrier substance. In particular, mixtures of paraffins and bistearylethylenediamide are preferred.

One Surfactant preparation according to the invention may further comprise an or contain several water-soluble salts, for example to adjust the viscosity. It can be about inorganic and / or organic salts act. Applicable inorganic Salts are preferably selected from the group comprising colorless water-soluble halides, sulphates, Sulfites, carbonates, bicarbonates, nitrates, nitrites, phosphates and / or oxides of alkali metals, alkaline earth metals, aluminum and / or the transition metals; furthermore, ammonium salts used. Particularly preferred are halides and sulfates the alkali metals; Preferably, therefore, the inorganic salt selected from the group comprising sodium chloride, potassium chloride, Sodium sulfate, potassium sulfate and mixtures thereof. Applicable organic Salts are, for example, colorless water-soluble alkali metal, Alkaline earth metal, ammonium, aluminum and / or transition metal salts the carboxylic acids. Preferably, the salts are selected from the group comprising formate, acetate, propionate, citrate, malate, Tartrate, succinate, malonate, oxalate, lactate and mixtures thereof.

For thickening, a surfactant formulation may contain one or more polymeric thickeners. Polymeric thickeners are the polyelectrolytes thickening polycarboxylates, preferably homopolymers and copolymers of acrylic acid, especially acrylic acid copolymers such as acrylic acid-methacrylic acid copolymers, and the polysaccharides, especially heteropolysaccharides, and other conventional verdi caking polymers. Suitable polysaccharides or heteropolysaccharides are the polysaccharide gums, for example gum arabic, agar, alginates, carrageenans and their salts, guar, guar gum, tragacanth, gellan, Ramzan, dextran or xanthan and their derivatives, eg. B. propoxylated guar, and their mixtures. Other polysaccharide thickeners, such as starches or cellulose derivatives, may be used alternatively, but preferably in addition to a polysaccharide gum, for example starches of various origins and starch derivatives, e.g. As hydroxyethyl starch, starch phosphate esters or starch acetates, or carboxymethyl cellulose or its sodium salt, methyl, ethyl, hydroxyethyl, hydroxypropyl, hydroxypropyl methyl or hydroxyethyl methyl cellulose or cellulose acetate.

A preferred polymeric thickener is the microbial anionic heteropolysaccharide xanthan gum, which is produced by Xanthomonas campestris and some other species under aerobic conditions with a molecular weight of 2-15 × 10 ^6, and for example, by Fa. Kelco under the trade name Keltrol ^®, z. As a cream-colored powder Keltrol ^® T (transparent) or as white granules Keltrol ^® RD (Readily Dispersable).

Examples of acrylic acid polymers which are suitable as polymeric thickeners are high molecular weight homopolymers of acrylic acid (INCI Carbomer) crosslinked with a polyalkenyl polyether, in particular an allyl ether of sucrose, pentaerythritol or propylene (INCI Carbomer), which are also referred to as carboxyvinyl polymers. Such polyacrylic acids are available, inter alia, from the company. BFGoodrich under the trade name Carbopol ^® , z. Carbopol ^® 940 (molecular weight approximately 4,000,000), Carbopol ^® 941 (molecular weight approximately 1,250,000) or Carbopol ^® 934 (molecular weight approximately 3,000,000).

Of the Content of polymeric thickener is usually not more than 8% by weight, preferably between 0.1 and 7% by weight, more preferably between 0.5 and 6 wt .-%, in particular between 1 and 5% by weight, and most preferably between 1.5 and 4% by weight, for example between 2 and 2.5% by weight.

A Surfactant preparation may further contain volatile alkali. As such, ammonia and / or alkanolamines containing up to 9 C atoms contained in the molecule can be used. As alkanolamines, the ethanolamines are preferred and of these again the monoethanolamine. The content of ammonia and / or alkanolamine is preferably 0.01 to 2 wt .-%; particularly preferred ammonia is used. In addition, even small quantities be included in bases. Preferred bases are from the group the alkali and alkaline earth metal hydroxides and carbonates, in particular the alkali metal hydroxides, of which potassium hydroxide and especially Sodium hydroxide is particularly preferred.

A surfactant preparation may also contain a hydrophilizing agent. These are understood in the context of the present invention means for the hydrophilization of surfaces. In particular, colloidal silica sols in which the silicon dioxide is present nanoparticulate are suitable for hydrophilization. Colloidal nanoparticulate silica sols for the purposes of this invention are stable dispersions of amorphous particulate silicon dioxide SiO ₂ having particle sizes in the range from 1 to 100 nm. The particle sizes are preferably in the range from 3 to 50 nm, particularly preferably from 4 to 40 nm a silica sol, which is suitable to be used for the purposes of this invention is that available under the trade name Bindzil ^® 30/360 from Akzo silica sol having a particle size of 9 nm. Further suitable silica sols are Bindzil ^® 15/500, 30/220, 40/200 (Akzo), Nyacol ^® 215, 830, 1430, and 2034Dl Nyacol ^® DP5820, DP5480, DP5540 etc. (Nyacol Products), Levasil ^® 100/30, 100F / 30, 100S / 30, 200/30, 200F / 30, 300F / 30, VP 4038, VP 4055 (HC Starck / Bayer) or CAB-O-SPERSE ^® PG 001, PG 002 (aqueous dispersion of CAB-O-SIL ^®, Cabot), Quartron PL-1, PL-3 (Fuso Chemical Co.), Köstrosol 0830, 1030, 1430 (Chemiewerk Bad Köstritz). The silica sols used may also be surface-modified silica treated with sodium aluminate (alumina-modified silica).

Besides Certain polymers can also be used to hydrophilize surfaces deploy. Hydrophilizing polymers are in particular amphoteric Suitable polymers, for example copolymers of acrylic or methacrylic acid and MAPTAC, DADMAC or another polymerizable quaternary Ammonium compound. Furthermore, copolymers with AMPS (2-acrylamido-2-methylpropanesulfonic acid) is used become. Polyethersiloxanes, ie copolymers of polymethylsiloxanes with ethylene oxide or propylene oxide segments are further suitable Polymers. Also usable are acrylic polymers, maleic acid copolymers and polyurethanes with PEG (polyethylene glycol) units. suitable Polymers are, for example, under the trade names Mirapol Surf-S 100, 110, 200, 210, 400, 410, A 300, A 400 (Rhodia), Tegopren 5843 (Goldschmidt), Sokalan CP 9 (BASF) or Polyquart Ampho 149 (Cognis) commercially available.

The to select ingredients of the surfactant preparation as well the conditions under which they are used according to the invention such as temperature, pH, ionic strength, Redox conditions or mechanical influences, are usually for the respective field of application optimized.

in the The present invention includes the hydrolytic enzyme and thus the virucidal treatment solution containing it a virus activity against at least one virus, preferred over several viruses. However, it is not mandatory that virus effectiveness against a virus belonging to the species poliovirus. Polioviruses may be of the invention Be excluded because they are constantly improving Vaccinations or vaccination campaigns are efficiently combated and are considered extinct almost worldwide. Another method of the invention is therefore characterized in that the method is not against a virus which belongs to the species Poliovirus. Such a method can therefore also be carried out with a virucidal treatment solution, which has viral activity against a virus that is not associated is a poliovirus species.

The Poliovirus is a virus belonging to the genus Enterovirus the family Picornaviridae. It triggers polio in humans or poliomyelitis. It is a very simple virus without envelope with a genome of single-stranded plus RNA. The approximately round virus particle has a diameter of 28 to 30 nanometers. Each virion contains a copy of the single-stranded RNA genome that is of an icosahedral Kapsid is wrapped. The capsid consists of 60 copies each of the four capsid proteins (VP1, VP2, VP3 and VP4) together. The virus has no shell. The poliovirus multiplies in the cytoplasm the host cell. To penetrate the cell, needed the virus has a specific receptor, the CD155 protein. Then can the virus transfers its RNA to the cell where it is immediately is translated to the polyprotein. The polyprotein can self proteolytically into single structural and functional proteins disassemble. The RNA is not only translated but also replicated. The latter is done by a viral RNA-dependent RNA polymerase (3Dpol) containing the original plus RNA in a minus RNA rewrites and immediately generates new plus RNA from it. The RNA eventually becomes one with the structural proteins packed new virion. Finally, the host cell dies and the viruses are released.

According to the invention preferred Methods are characterized in that the hydrolytic enzyme a Virus activity against a virus selected is from the group consisting of Adenoviridae, Alphaherpesvirinae, Astroviridae, Betaherpesvirinae, Birnaviridae, Bornaviridae, Bunyaviridae, Caliciviridae, Chordopoxviridae, Filoviridae, Flaviviridae, Gammaherpesvirinae, Hepadnaviridae, Herpesviridae, Iridoviridae, Orthomyxoviridae, Orthoretroviridae, Papillomaviridae, Paramyxovirinae, Parvovirinae, Picornaviridae, Pneumovirinae, Polyomaviridae, Reoviridae, Rhabdoviridae, Roniviridae, Spumaretroviridae and Togaviridae.

among them in particular, it is a virus belonging to it is one of the following genera: alpha papillomavirus, alpharetrovirus, Alphavirus, Aphthovirus, Aquabirnavirus, Aquareovirus, Avibirnavirus, Avulavirus, Betapapillomavirus, Betaretrovirus, Bocavirus, Bornavirus, Cardiovirus, coltivirus, cypovirus, cytomegalovirus, deltaretrovirus, Delta virus, Dependovirus, Ebola virus, Enterovirus, Ephemerovirus, Epsilon retrovirus, Erbovirus, Erythrovirus, Fijivirus, Flavivirus, Fungal Prions, Gammapapillomavirus, Gammaretrovirus, Hantavirus, Henipavirus, Hepacivirus, Hepatovirus, Hegevirus, Ictalurivirus, Idnoreovirus, Iflavirus, influenza A virus, influenza B virus, influenza virus C, Iridovirus, Kobuvirus, Lentivirus, Lymphocryptovirus, Lyssavirus, Mamastrovirus, Marburg virus, Mastadenovirus, Megalocytivirus, Morbillivirus, Mupapillomavirus, Muromegalovirus, Mycoreovirus, Nairovirus, Norovirus, Novirhabdovirus, Nupapillomavirus, Okavirus, Orbivirus, Orthobunyavirus, Orthohepadnavirus, Orthopoxvirus, Orthoreovirus, Oryzavirus, Parechovirus, Parvovirus, Pestivirus, Phlebovirus, Phytoreovirus, Pneumovirus, Polyomavirus, Respirovirus, Rhadinovirus, Rhinovirus, Roseolovirus, Rotavirus, rubivirus, rubulavirus, sapovirus, seadornavirus, simplexvirus, Spumavirus, teschovirus, varicellovirus, vesiculovirus, vesivirus.

The mentioned genera again comprise one or more virus species (Virus types), and these in turn can be different virus serotypes include. Serotypes are serologically distinguishable variations a virus. The serotype can be confirmed by serological tests (for example ELISA ("enzyme linked immunosorbent assay") become. Such serological tests are based on the specific ones Properties of the antibodies against certain surface structures (Antigens) of the virus particle are directed. Various viruses are coming in different forms with different types of antigens. The different tribes each represent an independent one Serotype, which are determined by means of a serological test can. The immune system treats every serotype of a virus as one immunologically distinct virus, allowing each serotype to a type-specific immunity leads. Separate Serotypes are therefore in particular on the basis of the directed against them Antibodies distinguishable.

The designation of the viruses is carried out according to the usual and established virus taxonomy of the International Committee on Taxonomy of Viruses ( ICTV, cf. in particular, CM Fauquet, MA Mayo et al .: Eighth Report of the International Committee to Taxonomy of Viruses, London, San Diego, 2004 ). In particular, this taxonomy considers the genome structure (DNA, RNA, single-stranded, double-stranded, polarity, linear, circular, segmented), the shape (symmetry) of the capsid, the presence of a shell, the location of the genes within the genome, the replication strategy, and virus size ,

For Viruses of the above virus families and virus genera was found that their disinfection on textiles and / or hard Surfaces due to the hydrolytic enzymes in the virucidal Treatment solution is particularly advantageous.

There the hydrolytic enzyme makes a significant contribution to disinfection of the textile or hard surface an inventive process under such conditions (such as temperature, pH, ionic strength, redox ratios), among which the hydrolytic enzyme is catalytically active. Vice versa For example, the hydrolytic enzyme can be chosen to be under the particular conditions under which the virucidal treatment solution is applied, is catalytically active. Preferably, an inventive Process in a temperature range between 10 ° C and 60 ° C, especially between 10 ° C and 50 ° C. and more preferably between 10 ° C and 40 ° C. Thermostable hydrolytic enzymes could even at higher temperatures than 60 ° C in the invention Be applied to, for example, up to 70 ° C or 75 ° C. The pH at which an inventive Method advantageously carried out may be dependent be of the invention to be disinfected Object. For example, a virucidal treatment solution, which is based on a detergent for toilets, advantageously an acidic pH, for example a pH between pH2 and pH5. A virucidal treatment solution on a Laundry detergent or a cleaning agent for other hard surfaces, advantageously has one slightly acidic, neutral or alkaline pH, for example a pH between pH6 and pH11 or between pH7 and pH10. A virucidal treatment solution based on a hand dishwashing liquid For example, it has a pH between pH 6.5 and pH 8.

In Another embodiment is an inventive A method characterized in that the virucidal treatment solution for at least 10 seconds and increasingly preferred for at least 15, 20, 25 and 30 seconds in contact with the textile or the hard surface is. This aspect concerns the minimum exposure time of the virucidal treatment solution on the virus to achieve a satisfactory viral efficacy. The exposure time can also be longer, for example at least 60, 90, 120, 150, 180, 210, 240, 270 or 300 seconds, or at least 10, 20, 30, 40, 50 or 60 minutes.

In In another embodiment, the hydrolytic Enzyme or the virucidal treatment solution containing the hydrolytic enzyme an RF value greater than one, determined according to the DVV / RKI Guideline. As explained above, the RF value a measure of the decrease in virus titer in the presence a hydrolytic enzyme or a virzuziden treatment solution, which contains the hydrolytic enzyme compared to a Water approach. The RF value is determined as described above. RF values greater than one indicate viral activity in this case, since the decrease of the virus titer in the test batch greater than the decrease in virus titer in the water batch is. Increasingly preferred, the hydrolytic enzyme or the the hydrolytic enzyme-containing virucidal treatment solution an RF value greater than or equal to 1.5, 2, 2.5, 3, 3.5 and more preferably greater than or equal to 4 on.

In a further preferred embodiment of an inventive Method, the hydrolytic enzyme is a protease, amylase, cellulase, Glycosidase, hemicellulase, mannanase, xylanase, pectinase, β-glucosidase, Carrageenase or a lipase or a mixture that at least includes two of these enzymes.

Because all of these enzymes have been found to have viral activity allowing them to disinfect viruses on textiles and / or hard Surfaces within the scope of the invention Methods are suitable. Particular preference is given to the hydrolytic enzyme to a protease, and preferably around a serine protease, more preferably a subtilase, and especially preferably a subtilisin.

Subtilases (also subtilopeptidases, EC 3.4.21.62) are serine proteases and act as nonspecific endopeptidases, that is, they hydrolyze any acid amide linkages that are internal to peptides or proteins. Their pH optimum is usually in the clearly alkaline range. An overview of this family offers for example the Article "Subtilases: Subtilisin-like Proteases" by R. Siezen, pages 75-95 in "Subtiltis enzymes", edited by R. Bott and C. Betzel, New York, 1996 , Subtilases are naturally produced by microorganisms. Among the subtilases, the most subtilisins formed and secreted by Bacillus species are the most important group.

numerous Proteases and especially subtilisins are called pre-proteins, so formed together with a propeptide and a signal peptide, the function of the signal peptide is usually therein the release of the protease from the one producing it Cell into the periplasm or to ensure the medium surrounding the cell and the propeptide usually for the correct one Conclusion of the protease is necessary. The signal peptide and the Propeptide are usually the N-terminal part of the preprotein. The Signal peptide and the propeptide become natural Conditions in the course of the secretion and folding process of the rest Protease split off. For inventive Methods are due to their enzymatic activity the mature proteases, d. H. the finished after their production processed enzymes without signal and propeptide, compared the preproteins are preferred. The proteases can from the cells producing them after the production of the polypeptide chain be modified, for example, by attaching Sugar molecules, formylations, aminations, etc. Such Post-translational modifications may need however, do not exert any influence on the function of the protease.

Examples of proteases are the subtilisins BPN 'from Bacillus amyloliquefaciens and Carlsberg from Bacillus licheniformis, the protease PB92, the subtilisins 147 and 309, the protease from Bacillus lentus, subtilisin DY and the subtilases, but no longer the subtilisins in the strict sense attributable enzymes Thermitase, proteinase K and the proteases TW3 and TW7. Subtilisin Carlsberg in further developed form under the trade name Alcalase ^® from the company Novozymes A / S, Bagsvaerd, Denmark. The subtilisins 147 and 309 are sold under the trade names Esperase ^®, or Savinase ^® by the company Novozymes. From the protease from Bacillus lentus DSM 5483 derived under the name BLAP ^® protease variants derived. Other usable proteases are, for example, under the trade names Durazym ^®, relase ^®, Everlase® ^®, Nafizym ^®, Natalase ^®, Kannase® ^® and Ovozyme ^® by the company Novozymes, the ^® under the trade names Purafect ^®, Purafect ^® OxP, Purafect Prime, Excellase ^® and Properase.RTM ^® by the company Danisco / Genencor, that under the trade name Protosol® ^® by the company Advanced Biochemicals Ltd., Thane, India, that the under the trade name Wuxi ^® from the company Wuxi Snyder Bioproducts Ltd., China, under trade names Proleather® ^® and protease P ^® by the company Amano Pharmaceuticals Ltd., Nagoya, Japan, and the enzymes available under the name proteinase K-16 by the company Kao Corp., Tokyo, Japan. Particular preference is also given to using the proteases from Bacillus gibsonii and Bacillus pumilus, which are disclosed in the international patent applications WO 08/086916 and WO 07/131656 , Further advantageous proteases are disclosed in the patent applications WO 91/02792 . WO 08/007319 . WO 93/18140 . WO 01/44452 . GB 1243784 . WO 96/34946 . WO 02/029024 and WO 03/057246 , Other useful proteases are those naturally present in the microorganisms Stenotrophomonas maltophilia, in particular Stenotrophomonas maltophilia K279a, Bacillus intermedius and Bacillus sphaericus.

Examples of amylases are the α-amylases from Bacillus licheniformis, from Bacillus amyloliquefaciens or from Bacillus stearothermophilus and, in particular, also their improved developments for use in detergents or cleaners. The enzyme from Bacillus licheniformis is available from the company Novozymes under the name Termamyl ^® and by the company Danisco / Genencor under the name Purastar® ^® ST. Development products of this α-amylase are available from the company Novozymes under the trade names Duramyl ^® and Termamyl ^® ultra, by the company Danisco / Genencor under the name Purastar® ^® OxAm and from the companies Daiwa Seiko Inc., Tokyo, Japan, as Keistase ^® available. The α-amylase from Bacillus amyloliquefaciens is marketed by the company Novozymes under the name BAN ^®, and variants derived from the α-amylase from Bacillus stearothermophilus under the names BSG ^® and Novamyl ^®, also from the company Novozymes. Furthermore, for this purpose, the α-amylase from Bacillus sp. A 7-7 (DSM 12368) and cyclodextrin glucanotransferase (CGTase) from Bacillus agaradherens (DSM 9948). Furthermore, the amylolytic enzymes used in the international patent applications WO 03/002711 . WO 03/054177 and WO 07/079938 are disclosed. Likewise, fusion products of all the molecules mentioned can be used. In addition, the enhancements available under the trade names Fungamyl.RTM ^® by the company Novozymes of α-amylase from Aspergillus niger and A. oryzae, which are. Further usable commercial products are, for example, the Amylase-LT ^® and Stainzyme ^® or Stainzyme ultra ^® or Stainzyme plus ^® , the latter also from the company Novozymes. Also variants of these enzymes obtainable by point mutations can be used according to the invention.

Examples of cellulases (endoglucanases, EC) is the fungal, endoglucanase (EG) -rich cellulase se-preparation or its further developments, which is offered by the company Novozymes under the trade name Celluzyme ^® . The products Endolase ^® and Carezyme ^® , also available from Novozymes, are based on the 50 kD EG or the 43 kD EG from Humicola insolens DSM 1800. Further commercial products of this company are Cellusoft ^® , Renozyme ^® and Cellucean ^® . Are still used, for example the 20 kD EG Melanocarpus that are available from the company AB Enzymes, Finland, under the trade names Ecostone® ^® and Biotouch ^®. Other commercial products of AB Enzymes are Econase ^® and Ecopulp ^® . Other suitable cellulases are from Bacillus sp. CBS 670.93 and CBS 669.93, those derived from Bacillus sp. CBS 670.93 by the company Danisco / Genencor under the trade name Puradax® ^® available. Further commercial products of the company Danisco / Genencor are "Genencor detergent cellulase L" and IndiAge ^® Neutra.

Further preferred hydrolytic enzymes are those identified by the term Glycosidases (E.C. 3.2.1.X) are summarized. Which includes in particular arabinases, fucosidases, galactosidases, galactanases, Arabico-galactan galactosidases, mannanases (also referred to as Mannosidases or mannases), glucuronosidases, agarase, carrageenases, Pullulanases, β-glucosidases, xyloglucanases (xylanases) and pectin degrading enzymes (pectinases). Preferred glycosidases are also summarized by the term hemicellulases. To Hemicellulases include, in particular, mannanases, xyloglucanases (Xylanases), β-glucosidases and carrageenases and also Pectinases, pullulanases and β-glucanases.

pectinases are pectin-degrading enzymes, with the hydrolytic pectin-degrading Enzymes in particular belong to the enzyme classes EC 3.1.1.11, EC 3.2.1.15, EC 3.2.1.67 and EC 3.2.1.82. other names pectin esterase, pectin methoxylase, pectin methoxylase, Pectin methyl esterase, pectase, pectin methyl esterase, pectin esterase, Pectinpectyl hydrolase, pectin polymerase, endopolygalacturonase, Pectolase, pectin hydrolase, pectin polygalacturonase, endo-polygalacturonase, Poly-α-1,4-galacturonide glycanohydrolase, endogalacturonase, Endo-D-galacturonase, galacturan 1,4-α-galacturonidase, exopolygalacturonase, Poly (galacturonate) hydrolase, exo-D-galacturonase, exo-D-galacturonanase, Exopoly-D-galacturonase, exo-poly-α-galacturonosidase, Exopolygalacturonosidase or Exopolygalacturanosidase.

In this regard, suitable enzymes United States for example, under the name Gamanase ^® and Pektinex AR ^® from the company Novozymes under the name Rohapec ^® B1L by the company AB Enzymes and under the name Pyrolase® ^® by the company Diversa Corp., San Diego, CA. , The recovered from Bacillus subtilis β-glucanase is available under the name Cereflo ^® by the company Novozymes. According to the invention particularly preferred glycosidases or hemicellulases are mannanases, which are marketed under the trade names man Away ^® by the company Novozymes or Purabrite ^® by the company Danisco / Genencor.

Examples of lipases or cutinases are the lipases originally obtainable from Humicola lanuginosa (Thermomyces lanuginosus) or developed therefrom, in particular those with the amino acid exchange D96L. They are sold, for example, by the company Novozymes under the trade names Lipolase ^®, Lipolase Ultra ^®, LipoPrime® ^®, Lipozyme® ^® and Lipex ^®.

Furthermore, for example, the cutinases can be used, which were originally isolated from Fusarium solani pisi and Humicola insolens. Also useful lipases are from the company Amano under the names Lipase CE ^® , Lipase P ^® , Lipase B ^® , or Lipase CES ^® , lipase AKG ^® , Bacillis sp. ^Lipase® , Lipase ^AP® , Lipase M- ^AP® and Lipase ^{AML® are} available. From the company Danisco / Genencor, for example, the lipases or cutinases can be used, the initial enzymes were originally isolated from Pseudomonas mendocina and Fusarium solanii. Other important commercial products are originally from the company Gist-Brocades (now Danisco / Genencor) marketed preparations M1 Lipase ^® and Lipomax® ^® and by the company Meito Sangyo KK, Japan under the names Lipase MY-30 ^®, Lipase OF ^® to mention and lipase PL ^® marketed enzymes, and also the product Lumafast® ^® by the company Danisco / Genencor.

Of all these enzymes, particular preference is given to those which are comparatively stable per se by oxidation or have been stabilized, for example, by mutations, in particular by substitution, deletion or insertion of one or more amino acids. For this purpose, the aforementioned commercial products Everlase ^® and Purafect OxP as examples of such proteases and Duramyl are particular to cite as an example of such an α-amylase.

The Enzymes used according to the invention are preferably derived originally from microorganisms, such as the genera Bacillus, Streptomyces, Humicola, or Pseudomonas, and / or are after known biotechnological processes by suitable microorganisms produced, for example by transgenic expression hosts of the genera Bacillus or filamentous mushrooms. It is emphasized in particular, they are also technical enzyme preparations of the particular hydrolytic enzyme, d. H. accompanying substances may be present. Therefore, the enzymes can be together with accompanying substances, for example from the fermentation or with stabilizers assembled and used.

Provided several hydrolytic enzymes according to the invention for Disinfecting viruses on textiles or hard surfaces applied, they show particular preference in this regard a synergistic effect. This means that the combination the hydrolytic enzymes used improved viral efficacy has in comparison with the virus activity of one each these enzymes, or even compared to the expected virus activity the enzymes due to the mere addition of each Individual contributions of these enzymes to viral activity. All more preferably such a synergy exists between two Proteases or between a protease and an amylase and / or a mannanase and / or a lipase.

One Another object of the invention is a process for the preparation a virucidal treatment solution for the disinfection of Textiles and / or hard surfaces, characterized is that the treatment solution at least one hydrolytic Enzyme is added.

All Facts, objects and embodiments, the above for inventive method are also applicable to this subject invention. Therefore, explicit reference is made to the revelation at this point in the appropriate place with the reference that this disclosure also for the above inventive Process for the preparation of a virucidal treatment solution for disinfecting textiles and / or hard surfaces applies.

Example:

The virus potency of the following hydrolytic enzymes was determined according to the DVV / RKI guideline ( German Association for the Control of Viral Diseases e. V./Robert-Koch-Institut; see. Federal Health Gazette (2005), 48, 1420-1426 ), ie the decrease of the virus titer of the examined virus in the presence of the respective hydrolytic enzyme was determined in comparison to the decrease of the virus titer of the examined virus in a water batch without the hydrolytic enzyme.

All Test solutions were in dest. Water attached. Incubation was at 40 ° C for those in the following Table given times. The application concentration of the hydrolytic Enzyme was 100 ppm. For some enzymes were added Measurements carried out at an application concentration of 1 ppm. The for the tiger determination according to the DVV / RKI guideline used host cells are in the following Tables 1 and 2 are also given.

• 1. Variante F49 der Protease aus Bacillus lentus gemäß WO 95/23221
• 2. Everlase^® 16 LEX (Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)
• 3. Alcalase^® 2,5 L (Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)
• 4. Stainzyme^® 12.0 L(Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)
• 5. Ecostone^® N400 (AB Enzymes GmbH, Feldbergstrasse 78, 64293 Darmstadt, Deutschland)
• 6. Carezyme^® 4500 L (Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)
• 7. Mannaway^® 4.0 T (Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)
• 8. Lipolase^® 100 L (Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Dänemark)

The following enzymes were used:

• 1. variant F49 of the protease from Bacillus lentus according to WO 95/23221
• 2. Everlase ^® 16 LEX (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)
• 3. Alcalase ^® 2.5 L (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)
• 4. Stainzyme ^® 12.0 L (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)
• 5. Ecostone® ^® N400 (AB Enzymes GmbH, Feldbergstrasse 78, 64293 Darmstadt, Germany)
• 6. Carezyme ^® 4500 L (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)
• 7. Mannaway ^® 4.0 T (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)
• 8. Lipolase ^® 100L (Novozymes A / S, Krogshoejvej 36 2880 Bagsvaerd, Denmark)

The Results are shown in Tables 1 and 2 below as RF values shown.

Duplicate determinations are separated by slashes. It is clear that the hydrolytic enzymes, especially after 30 minutes incubation period have a significant virus activity against the indicated virus (indicated by RF values greater than or equal to one, in particular RF values greater than two or even greater than three). Thus, hydrolytic enzymes can be used virus-effectively in the context of inventive method for the disinfection of textiles and / or hard surfaces. Table 1: Concentration (ppm) Incubation period (min) RF-value (s) virus CV B3 (Enterovirus, Hum Coxsackievirus B3) host cell GMK Enzyme 1 100 15 3.1 / 3.0 30 4.1 / 4.4 Enzyme 2 100 15 3.6 30 3.5 Enzyme 3 100 15 3.6 / 3.5 30 4.1 / 4.0 Enzyme 4 100 15 4.0 30 4.0 Enzyme 5 100 15 1.6 30 3.8 Enzyme 6 100 15 4.0 30 4.3 Enzyme 7 100 15 3.6 30 3.8 Enzyme 8 100 15 3.5 30 4.3 Table 2: Concentration (ppm) Incubation period (min) RF-value (s) virus SV 40 (polyoma virus, simian virus 40 pieces 777) VACV (Orthopoxvirus, Vacciniavirus St. Elstree) HFIuAV influenza virus (Hum. A H3N2, California 7/04) host cell CV1 Vero MDCK Enzyme 1 100 15 3.0 / 3.0 0.5 / 0.4 30 4.5 / 3.8 1.1 / 1.0 1 30 3.0 Enzyme 2 100 15 4.4 0.5 30 5.4 2.4 1 30 3.0 Enzyme 3 100 15 4.8 / 3.4 1.4 / 0.5 30 4.6 / 3.6 2.8 / 2.5 1 30 3.0

QUOTES INCLUDE IN THE DESCRIPTION

This list The documents listed by the applicant have been automated generated and is solely for better information recorded by the reader. The list is not part of the German Patent or utility model application. The DPMA takes over no liability for any errors or omissions.

Cited patent literature

• - EP 0300305 [0032]
• - DE 2412837 [0040]
• WO 08/086916 [0074]
• WO 07/131656 [0074]
• WO 91/02792 [0074]
• WO 08/007319 [0074]
• WO 93/18140 [0074]
• WO 01/44452 [0074]
• GB 1243784 [0074]
• WO 96/34946 [0074]
• WO 02/029024 [0074]
• WO 03/057246 [0074]
• WO 03/002711 [0075]
• WO 03/054177 [0075]
• WO 07/079938 [0075]
• WO 95/23221 [0089]

Cited non-patent literature

• Kessler & Richards, Symbollon Corp., Sudbury, 1993 [0005]
• Guideline of the German Association for the Control of Viral Diseases e. V. and the Robert Koch Institute for the testing of chemical disinfectants for effectiveness against viruses in human medicine; Version of 15 June 2005, Bundesgesundheitsblatt (Bundesgesundheitsblatt 48, (2005) 1420-1426) [0011]
• Gornall AG, CS Bardawill and MM David, J. Biol. Chem., 177 (1948), pp. 751-766 [0023]
• - ICTV, cf. in particular, CM Fauquet, MA Mayo et al .: Eighth Report of the International Committee to Taxonomy of Viruses, London, San Diego, 2004 [0065]
• - Article "Subtilases: Subtilisin-like Proteases" by R. Siezen, pages 75-95 in "Subtilisin enzymes", edited by R. Bott and C. Betzel, New York, 1996 [0072]
• - German Association for the Control of Viral Diseases e. V./Robert-Koch-Institut; see. Federal Health Gazette (2005), 48, 1420-1426 [0087]

Claims (11)

Method for disinfecting textiles and / or hard surfaces, characterized in that the textile and / or the hard surface is brought into contact with a virucidal treatment solution which comprises at least one hydrolytic enzyme. Method according to claim 1, characterized in that that the hydrolytic enzyme in the virucidal treatment solution in a concentration of 0.000005-0.1 g dry enzyme per 100 g of treatment solution and more preferably from 0.0005-0.1 g dry enzyme per 100 g treatment solution and 0.005-0.05 g dry enzyme per 100 g treatment solution is present. Method according to claim 1 or 2, characterized that the virucidal treatment solution further comprises at least one further disinfecting ingredient. Method according to one of claims 1 to 3, characterized in that the virucidal treatment solution further comprising a surfactant preparation, in particular a dilute one or undiluted washing, cleaning, Textilvor- or Aftertreatment agent or disinfectant. Method according to claim 4, characterized in that the surfactant preparation further comprises at least one further ingredient preferably one selected from the group consisting of surfactant, builder, peroxygen compound, bleach activator, Alcohol, acid, grayness inhibitor, optical brightener, foam inhibitor, water-soluble salt, polymeric thickener, volatile Alkali and / or base, hydrophilizing agent and combinations hereof. Method according to one of claims 1 to 5, characterized in that the hydrolytic enzyme has a virus activity against a virus selected from the group consisting of Adenoviridae, Alphaherpesvirinae, Astroviridae, Betaherpesvirinae, Birnaviridae, Bornaviridae, Bunyaviridae, Caliciviridae, Chordopoxviridae, Filoviridae, Flaviviridae, Gammaherpesvirinae, Hepadnaviridae, Herpesviridae, Iridoviridae, Orthomyxoviridae, Orthoretroviridae, Papillomaviridae, Paramyxovirinae, Parvovirinae, Picornaviridae, Pneumovirinae, Polyomaviridae, Reoviridae, Rhabdoviridae, Roniviridae, Spumaretroviridae and Togaviridae. Method according to one of claims 1 to 6, characterized in that it is in a temperature range between 10 ° C and 60 ° C, especially between 10 ° C. and 50 ° C, and more preferably between 10 ° C and 40 ° C takes place. Method according to one of claims 1 to 7, characterized in that the virucidal treatment solution for at least 10 seconds and increasingly preferred for at least 15, 20, 25 and 30 seconds in contact with the textile or the hard surface is. Method according to one of claims 1 to 8, characterized in that the hydrolytic enzyme or the the hydrolytic enzyme-containing virucidal treatment solution has an RF value greater than one, determined according to DVV / RKI Guideline. Method according to one of claims 1 to 9, characterized in that the hydrolytic enzyme is a protease, Amylase, cellulase, glycosidase, hemicellulase, mannanase, xylanase, Pectinase, β-glucosidase, carrageenase or a lipase or a mixture comprising at least two of these enzymes, in particular a protease, preferably a serine protease, more preferably a Subtilase, and more preferably a subtilisin. Process for the preparation of a virucidal treatment solution for the disinfection of textiles and / or hard surfaces, characterized in that the treatment solution at least a hydrolytic enzyme is added.