CN2672632Y - Optical fiber continuous exciting microscopic fluorescent spectrum imaging instrument - Google Patents
Optical fiber continuous exciting microscopic fluorescent spectrum imaging instrument Download PDFInfo
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- CN2672632Y CN2672632Y CN 200320130965 CN200320130965U CN2672632Y CN 2672632 Y CN2672632 Y CN 2672632Y CN 200320130965 CN200320130965 CN 200320130965 CN 200320130965 U CN200320130965 U CN 200320130965U CN 2672632 Y CN2672632 Y CN 2672632Y
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- microscope
- optical fiber
- illumination device
- monochromatic illumination
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Abstract
The utility model discloses an imaging instrument continuously exciting microfluorescent optical spectrum with optical fibers, comprising an automatic linear variable monochromatic illumination device for a microscope, a fluorescence microscope, an image adapter and a CCD image pick-up device successively connected to an optical path, also an image grabber and a computer which are arranged on a circuit and are successively connected between the CCD image pick-up device and the automatic linear variable monochromatic illumination device for a microscope through data lines, and finally an image display which is arranged on the circuit and connected with the computer through data lines. The optical spectrum monochromator of the automatic linear variable monochromatic illumination device for a microscope has a relative aperture f/#=3.1, the focal-length of a condensing concave mirror of 120 mm and an optical grating of 1200l/mm. The utility model can find the optimal excitation wavelength of any point on test specimens, obtain qualitative, quantitative and positioning analytical results of a specific object and simultaneously realize micro-fluorescence image analysis and micro-fluorescence spectral analysis on one fluorescence microscope.
Description
Technical field
The utility model relates to a kind of optical spectrum imagers, and especially continuously adjustable microscopic fluorescent spectral imaging instrument belongs to the physical optics field.
Background technology
In traditional microscopic fluorescence picture system, fluorescent microscope forms the amplification fluoroscopic image of observed sample; This fluoroscopic image is to make it emitting fluorescence by a kind of wavelength excitation light irradiation sample that fluorescence excitation light source and filter set are selected to form.As want sample under the different wave length irradiation, to excite the fluorescence of different wave length, then need to dispose different filter set.Because structural reason of fluorescent microscope or reason economically (the filter set price is higher), usually only dispose 2 to 3 groups of filter set on the general epifluorescence microscope, thereby the exciting light (being generally three kinds of ultraviolet, blue light and green glows) of 2 to 3 kinds of different wavelength range can only be provided; High-grade microscope can provide 4 to 5 kinds even more filter set, but the price of these filter set may will reach the value of microscope itself even higher.In addition, even the many groups of configuration filter set, also can only reach has a grade light modulation, can not obtain the stepless dimming effect that excitation wavelength can continuously change in needing scope; And can run into the fluorescent material of the different best excitation wavelengths of increasing needs in modern biology, medical science, medicine, new material research, clinical examination and the production, or do not know the sample of maximum excitation wavelength or can launch the sample of different fluorescence in different excitation wavelengths.Therefore, research and development can provide continuously adjustable micro-fluorescence spectrum picture system very necessary.
The utility model content
Technical problem to be solved in the utility model is to overcome above-mentioned the deficiencies in the prior art, the optical fiber continuous agitation microscopic fluorescent spectral imaging instrument that provides a kind of excitation wavelength can continuously change in needing scope.
For solving the problems of the technologies described above, optical fiber continuous agitation microscopic fluorescent spectral imaging instrument of the present utility model, be included in the microscope variable monochromatic illumination device of auto linear, fluorescent microscope, image adapter and the CCD picture pick-up device that connect successively on the light path, be connected to described CCD picture pick-up device and microscope on the circuit in turn by data line with image pick-up card and computing machine between the variable monochromatic illumination device of auto linear, and the image display that on circuit, is connected with described computing machine by data line;
The described microscope variable monochromatic illumination device of auto linear, comprise light source, have seam and go out the spectrum monochromator of seam and be arranged on described light source and the spectrum monochromator between condenser system, it is characterized in that, it also comprises the pure silica fibre of ultraviolet, one end of the saturating pure silica fibre of ultraviolet is connected with the seam that goes out of described spectrum monochromator by optical fiber interface, and the other end of the saturating pure silica fibre of ultraviolet is connected with microscopical lamp house by the optical fiber coupling interface; In the described spectrum monochromator, the grating and the optically focused concave mirror that on light path, be disposed with the reflective concave surface mirror, are connected with the axle of stepper motor, the relative aperture f/#=3.1 of spectrum monochromator, optically focused concave mirror focal length is 120mm, grating is the 1200l/mm grating;
Catoptron in the described fluorescent microscope is to the translucent half reflection eyeglass of the insensitive neutrality of wavelength value.
Described microscope is can be 100 watts ultrahigh pressure mercury lamp with the light source of the variable monochromatic illumination device of auto linear, also can be 100 watts UHV (ultra-high voltage) xenon lamp.
Described fluorescent microscope is to fall to penetrating the formula fluorescent microscope.
Compared with prior art, the utlity model has following beneficial effect: (1) can obtain the fluorescence intensity variation spectral line of any point when different excitation wavelength on the sample micro-fluorescence spectrum image, thereby can find the maximum excitation wavelength of any point on this sample, concrete object is obtained qualitative, quantitatively and the positioning analysis result; (2) in the fluorescent microscope, adopt the translucent half reflection eyeglass of the insensitive neutrality of wavelength value is replaced the dichroic mirror that only specific wavelength is had transmission/reflection characteristics, can be suitable for, need not change dichroic mirror the long scope of 250～680nm all-wave; (3) can save the exciter filter group; (4) the monochromatic spectrum scope can expand to ultraviolet; (5) can in 250～680nm wavelength coverage, provide continuously adjustable any wavelength, can directly be coupled into the different wave length exciting light that falls to penetrating the formula fluorescent microscope; (6) can realize that wavelength scans repeatedly, fluorescence such as standing wave length is paid and sent out, wavelength stepping pay and send out a function; (7) on a fluorescent microscope, realize microscopic fluorescence graphical analysis (obtaining image information) and micro-fluorescence spectrum analysis (obtaining composition and content information) simultaneously.
Description of drawings
Fig. 1 is microscope variable monochromatic illumination device of auto linear and a microscopical connection diagram.
Fig. 2 is the optical fiber circular open connection end view of microscope with the variable monochromatic illumination device of auto linear.
Fig. 3 is optical fiber and the connection end view thereof of microscope with the variable monochromatic illumination device of auto linear.
Fig. 4 is the optical fiber rectangular aperture connection end view of microscope with the variable monochromatic illumination device of auto linear.
Fig. 5 is the functional-block diagram of microscope with the function control device of the variable monochromatic illumination device of auto linear.
Fig. 6 is a functional-block diagram of the present utility model.
Reference numeral: 1, light source 2, condenser system 3, go into seam 4, optically focused concave mirror 5, grating 6, reflective concave surface mirror 7, go out seam 8, optical fiber interface 9, the saturating pure silica fibre 10 of ultraviolet, optical fiber coupling interface 11, fluorescent microscope 12, spectrum monochromator 13, coupling imaging len
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is elaborated.
Automatic wavelength is selected rather than manually filter set switching if satisfy, and at first must obtain monochromatic light.Adopt grating dispersion type spectrophotometric spectra monochromator to obtain monochromatic light at this.But because will be with monochromatic light as microscopical lighting source, it must have enough big luminous flux just can observe sample or sample institute emitted fluorescence, and this can realize by two approach: the one, infinitely increase the power of light source (mercury lamp or xenon lamp); It two is to adopt bigger relative aperture f/#, thereby collects the incident luminous energy more.Increase a series of problems such as selection that light source power relates to heat radiation and optical element, can not infinitely increase, and the light source of fluorescent microscope is exactly the ultrahigh pressure mercury lamp (HBO) of 100W usually, therefore we have adopted second kind of approach that increases luminous flux, the f/#=3.1 of the spectrum monochromator of redesign and production, grating 1200l/mm, optically focused concave mirror focal length is 120mm.Experiment showed, that this monochromator can satisfy lighting requirement substantially.
How the spectrum monochromator is connected with microscopical, and a kind of is that direct rigid mechanical connects, and a kind of is the soft connection of adopting optical fiber.Need to increase corresponding optical system owing to be rigidly connected, and make the bulky heaviness of instrument, so consider the flexible connection as shown in Figure 3 of use optical fiber.The connection end face of optical fiber one end is fixed on the seam that of spectrum monochromator, and this optical fiber connects the opening that end face has rectangle, as shown in Figure 4; The optical fiber link mask of the other end has circular open, as shown in Figure 2, is connected with microscope by the coupling imaging len that sets within it, thus the soft connection of realization optical fiber.
Optical fiber continuous agitation microscopic fluorescent spectral imaging instrument The general frame of the present utility model shown in Figure 6, be included in the microscope variable monochromatic illumination device of auto linear, fluorescent microscope, image adapter and the CCD picture pick-up device that connect successively on the light path, be connected to described CCD picture pick-up device and microscope on the circuit in turn by data line with image pick-up card and computing machine between the variable monochromatic illumination device of auto linear, and the image display that on circuit, is connected with described computing machine by data line.
Microscope is used to fluorescent microscope to provide wavelength continuously adjustable monochromatic excitation light with the variable monochromatic illumination device of auto linear, comprise that as shown in Figure 1 light source 1, condenser system 2, spectrum monochromator 12, the saturating pure silica fibre 9 of ultraviolet are connected end face 8,10 with optical fiber, and function control device shown in Figure 5.Wherein, spectrum monochromator 12 is by going into seam 3, reflective concave surface mirror 6, grating 5, optically focused concave mirror 4, going out seam 7 and form; Be provided with coupling imaging len 13 in the optical fiber connection end face 10.
Light source 1 can adopt 100 watts ultrahigh pressure mercury lamp, also can adopt 100 watts UHV (ultra-high voltage) xenon lamp, to produce the required enough luminous fluxes of spectrum monochromator 12.Condenser system 2 can be made of 2 convex lens, and the complex light light beam that is used for light source 1 is produced focuses on, and obtains a very little pointolite going into seam 3 places, and the complex light light beam after the focusing is injected in the spectrum monochromator 12 through going into seam 3.
One end of the saturating pure silica fibre 9 of ultraviolet connects end face 8 by optical fiber and is connected with the seam 7 that goes out of spectrum monochromator 12, is used for and will be coupled to optical fiber 9 and output at the monochromatic light that goes out seam 7 places, and optical fiber connects the opening that end face 8 has rectangle; Other end optical fiber connects end face 10 and is connected with described fluorescent microscope is soft, is provided with coupling imaging len 13 in the optical fiber connection end face 10, and coupling imaging len 13 is used for the monochromatic light of Optical Fiber Transmission is coupled to fluorescent microscope.
Function control device shown in Figure 5, digital indicator, input keyboard, motor driver and the communication interface that is connected with microprocessor respectively by microprocessor, by data line and form with the stepper motor that described motor driver is connected by data line, microprocessor, digital indicator, motor driver and stepper motor also are connected with power supply respectively.The microprocessor internal memory contains Control Software, is used to realize the parameter setting, moves to functions such as specified wavelength and dual wavelength scanning.Motor driver drives grating 5 by drive stepping motor and rotates under the control of microcontroller, variable with the auto linear of realizing monochromatic wavelength in the spectrum monochromator 12.Communication interface is used for carrying out exchanges data with external unit and network.
Buttons such as resetting, move to specified wavelength scanning, scanning, function setting option is arranged on the panel of function control device, function such as can realize that standing wave long scan, double wave scanning wavelength, stepping are moved can be provided with sweep velocity, stop parameters such as interval time, step direction, display mode.The monochromatic light that obtains certain specified wavelength both can be by controller and compunication control, also direct input instruction on the slave controller.
Silica fibre shown in Fig. 2,3,4 and connectivity port thereof, an end of the saturating pure silica fibre 9 of ultraviolet connects end face 8 by rectangular aperture and is connected with the seam 7 that goes out of spectrum monochromator 12, is used for and will be coupled to optical fiber 9 and output at the monochromatic light that goes out seam 7 places; The other end connects end face 10 by circular open and is connected with described fluorescent microscope is soft, is provided with coupling imaging len 13 in the optical fiber connection end face 10, and coupling imaging len 13 is used for the monochromatic light of Optical Fiber Transmission is coupled to fluorescent microscope.
Microscope is as follows with the principle of work of the variable monochromatic illumination device of auto linear, the light that light source 1 ultrahigh pressure mercury lamp or (UHV (ultra-high voltage) xenon lamp) send, through condenser system 2, seam 3 places of going at spectrum monochromator 12 obtain a very little pointolite, the optically focused concave mirror 4 of spectrum monochromator 12, grating 5, reflective concave surface mirror 6 are formed grating dispersion type beam splitting system together, obtain monochromatic light going out seam 7 places.The adjustment of wavelength is to drive grating 5 rotations by the stepper motor under microprocessor control to finish, because grating dispersion is equally distributed according to wavelength on imaging spectrum face, the sine value that goes out the corner of seam place light wavelength and grating 5 is a linear relationship, therefore as long as adjust the rotation step number of stepper motor, just can obtain the monochromatic light of different wave length linear variable.Monochromatic light is to export by the pure silica fibre 9 of saturating ultraviolet that the optical fiber that is fixed on out seam 7 places connects in the end face 8.
When microscope is connected with microscope 11 with the variable monochromatic illumination device of auto linear, need the lamp house of fluorescent microscope 11 is taken off, make optical fiber connect end face 10 by wherein coupling image-forming objective lens 13 and the fluorescence microscopy endoscope objective lens after be imaged on the place that is slightly larger than working distance of microscope, slight again moving fiber end face makes monochromation illumination even as far as possible.Sample can be observed or CCD opto-electronic conversion and image capturing system acquisition digitized image by eyepiece.
Between 250nm～680nm, the feasible monochromatic wavelength that is obtained of grating dispersion system is with the rotation step number linear change of stepper motor, therefore, monochromatic linear variable adjustment can realize by the rotation step number that stepper motor is set under microprocessor control.The monochromatic light that obtains certain specified wavelength both can be by controller and compunication control, also direct input instruction on the slave controller.Controller has function such as reset, the scanning of standing wave long scan, dual wavelength, stepping are moved, and sweep velocity can be set, stop parameters such as interval time, step direction, display mode.
Related hospitals is utilized dyeing test pieces such as the observation by microscope monkey liver of this monochromatic illumination device, caryogram fluorescence such as coarse particle type, fine grained type, flagellate have been observed, the spectrum picture in 250～680nm scope can be reappeared behind the digital imagery, particularly ultraviolet excited fluorescence can be observed.
Fall to penetrating the formula fluorescent microscope and be used for monochromatic excitation light is projected sample downwards, and collect the fluorescence formation microscopic fluorescence image that sample sends everywhere, the catoptron in the fluorescent microscope is to the translucent half reflection eyeglass of the insensitive neutrality of wavelength value.The image adapter can be made up of 2 convex lens, and the microscopic fluorescence image imaging that is used for fluorescent microscope is formed is to the CCD picture pick-up device.
The CCD picture pick-up device is used for converting the light signal of microscopic fluorescence image to corresponding analog electrical signal.Employing can receive the light signal of each point on the plane simultaneously and convert the area array CCD photoelectricity picture pick-up device of corresponding electric signal to, can form a series of two-dimentional fluoroscopic image under continuous variable wavelength or the some different wave length exciting lights, thereby realize different observation purposes.
Image pick-up card is used for converting the analog electrical signal of microscopic fluorescence image to digital electric signal, export described computing machine to, export described image display after computing machine is handled the digital electric signal of microscopic fluorescence image to and show, computing machine is also operated control to transmission, the storage of the electric signal of its microscopic fluorescence image.
Claims (4)
1. optical fiber continuous agitation microscopic fluorescent spectral imaging instrument, it is characterized in that, it is included in the microscope variable monochromatic illumination device of auto linear, fluorescent microscope, image adapter and the CCD picture pick-up device that connects successively on the light path, be connected to described CCD picture pick-up device and microscope on the circuit in turn by data line with image pick-up card and computing machine between the variable monochromatic illumination device of auto linear, and the image display that on circuit, is connected with described computing machine by data line;
The described microscope variable monochromatic illumination device of auto linear, comprise light source, have seam and go out the spectrum monochromator of seam and be arranged on described light source and the spectrum monochromator between condenser system, it is characterized in that, it also comprises the pure silica fibre of ultraviolet, one end of the saturating pure silica fibre of ultraviolet is connected with the seam that goes out of described spectrum monochromator by optical fiber interface, and the other end of the saturating pure silica fibre of ultraviolet is connected with microscopical lamp house by the optical fiber coupling interface; In the described spectrum monochromator, the grating and the optically focused concave mirror that on light path, be disposed with the reflective concave surface mirror, are connected with the axle of stepper motor, the relative aperture f/#=3.1 of spectrum monochromator, optically focused concave mirror focal length is 120mm, grating is the 1200l/mm grating;
Catoptron in the described fluorescent microscope is to the translucent half reflection eyeglass of the insensitive neutrality of wavelength value.
2. a kind of optical fiber continuous agitation microscopic fluorescent spectral imaging instrument according to claim 1 is characterized in that: described microscope is 100 watts ultrahigh pressure mercury lamp with the light source of the variable monochromatic illumination device of auto linear.
3. a kind of optical fiber continuous agitation microscopic fluorescent spectral imaging instrument according to claim 1 is characterized in that: described microscope is 100 watts UHV (ultra-high voltage) xenon lamp with the light source of the variable monochromatic illumination device of auto linear.
4. a kind of optical fiber continuous agitation microscopic fluorescent spectral imaging instrument according to claim 1, it is characterized in that: described fluorescent microscope is to fall to penetrating the formula fluorescent microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320130965 CN2672632Y (en) | 2003-12-31 | 2003-12-31 | Optical fiber continuous exciting microscopic fluorescent spectrum imaging instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320130965 CN2672632Y (en) | 2003-12-31 | 2003-12-31 | Optical fiber continuous exciting microscopic fluorescent spectrum imaging instrument |
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| CN2672632Y true CN2672632Y (en) | 2005-01-19 |
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| CN 200320130965 Expired - Fee Related CN2672632Y (en) | 2003-12-31 | 2003-12-31 | Optical fiber continuous exciting microscopic fluorescent spectrum imaging instrument |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175316A (en) * | 2011-01-19 | 2011-09-07 | 天津大学 | X-Y galvanometer scanning hyper spectral image data acquisition method |
| CN107607200A (en) * | 2017-08-07 | 2018-01-19 | 中国科学院高能物理研究所 | A kind of straight drive multichannel monochromator |
| CN111521266A (en) * | 2020-04-23 | 2020-08-11 | 佛山科学技术学院 | Spectrometer light path building device |
| CN113280917A (en) * | 2021-05-19 | 2021-08-20 | 苏州灵析精密仪器有限公司 | High resolution wide spectrum calibration light source |
-
2003
- 2003-12-31 CN CN 200320130965 patent/CN2672632Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175316A (en) * | 2011-01-19 | 2011-09-07 | 天津大学 | X-Y galvanometer scanning hyper spectral image data acquisition method |
| CN102175316B (en) * | 2011-01-19 | 2012-07-18 | 天津大学 | X-Y galvanometer scanning hyper spectral image data acquisition method |
| CN107607200A (en) * | 2017-08-07 | 2018-01-19 | 中国科学院高能物理研究所 | A kind of straight drive multichannel monochromator |
| CN107607200B (en) * | 2017-08-07 | 2019-09-20 | 中国科学院高能物理研究所 | A kind of straight drive multichannel monochromator |
| CN111521266A (en) * | 2020-04-23 | 2020-08-11 | 佛山科学技术学院 | Spectrometer light path building device |
| CN113280917A (en) * | 2021-05-19 | 2021-08-20 | 苏州灵析精密仪器有限公司 | High resolution wide spectrum calibration light source |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |