CN218834554U

CN218834554U - Multi-component storage and release device and microfluidic system

Info

Publication number: CN218834554U
Application number: CN202222941300.2U
Authority: CN
Inventors: 许家琦; 杨阳; 刘宇卉; 李临
Original assignee: Kemei Boyang Diagnostic Technology Shanghai Co ltd
Current assignee: Kemei Boyang Diagnostic Technology Shanghai Co ltd
Priority date: 2022-11-04
Filing date: 2022-11-04
Publication date: 2023-04-11
Anticipated expiration: 2032-11-04

Abstract

The utility model relates to a multicomponent is preserved and release arrangement and micro-fluidic system, multicomponent is preserved and release arrangement includes: the kit comprises a plurality of reagent boxes, wherein each reagent box is internally provided with an accommodating cavity and comprises a side surface provided with slots and/or inserting parts, and the inserting part of one reagent box can be inserted into the slot of the other reagent box; and the control valve is connected to the lower end of the accommodating cavity. The kit in the multicomponent is preserved and the release gear can be assembled as required to form different reagent and deposit the combination, satisfy different kinds of micro-fluidic chip test demands, avoid appearing multicomponent and preserve and the unmatched situation of release gear and micro-fluidic chip. The holding cavity can hold reagents required by multiple tests, and a preset amount of reagents can be released as required before the tests at every time, so that the waste of the surplus of pre-buried single test reagents is avoided, the utilization rate of the reagents is improved, the waste of the reagents is reduced, and the cost of the microfluidic chip is reduced.

Description

Multi-component storage and release device and microfluidic system

Technical Field

The utility model relates to a test technical field especially relates to a multicomponent is preserved and release and micro-fluidic system.

Background

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. The micro-fluidic chip can integrate various biological or chemical procedures on one chip to finish, and can accurately control micro volume reaction. A plurality of microfluidic chips select to embed reagents in the chips in advance, for example, a patent with the publication number of CN214716735U discloses a reagent embedding and sample injection device, the reagent embedding and sample injection device comprises a sample injection seat with a cavity structure, a reagent bag is placed at an open end of the top of the sample injection seat, a liquid injection port at the bottom of the sample injection seat is communicated with a gap cavity of the digital microfluidic chip, a needling component is arranged below the reagent bag, the reagent bag is extruded during sample injection, the reagent bag is punctured by the needling conducting component, and the reagents in the reagent bag flow into a reaction cavity of the digital microfluidic chip through the liquid injection port at the bottom of the sample injection seat. The reagent and other substances required by detection are quantitatively sealed in the reagent package in advance, and the reagent package is pre-embedded in the sample injection seat in advance and is sealed with the digital microfluidic chip integrally.

However, in the prior art, a plurality of reagents are respectively embedded in the microfluidic chip, so that in order to avoid the situation that the reagent amount is not enough in each test, the reagent allowance needs to be reserved for each reagent in each test, which causes great reagent waste.

Therefore, a multi-component storage and release device and a microfluidic system are needed to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a multicomponent is preserved and release device to preserve and release reagent.

To achieve the above object, the present invention provides in a first aspect a multicomponent storing and releasing device comprising:

the kit comprises a plurality of reagent boxes, wherein each reagent box is internally provided with an accommodating cavity and comprises a side surface provided with slots and/or inserting parts, and the inserting part of one reagent box can be inserted into the slot of the other reagent box;

and the control valve is connected to the lower end of the accommodating cavity.

Optionally, a limiting portion is arranged in the slot, and the limiting portion can limit the final position of the insertion portion in the slot.

Optionally, the size of the opening end of the cross section of the slot is L1, the size of the bottom of the cross section of the slot is L2, the size of the root of the cross section of the inserting part is L3, the size of the end of the cross section of the inserting part is L4, and L3 is greater than L1 and less than L4 and less than L2.

Optionally, the cross sections of the slot and the insertion part are both trapezoidal or convex.

Optionally, the kit is provided with a plurality of accommodating cavities arranged at intervals.

Optionally, the kit is a prism;

the slots are formed in at least two side faces; or

The insertion parts are arranged on at least two side surfaces.

Optionally, the multicomponent preserving and releasing device further comprises a pushing mechanism, wherein the pushing mechanism can be connected to the upper end of the containing cavity in an airtight manner; the pushing mechanism is an injection pump, a pneumatic pump or a peristaltic pump.

Optionally, the control valve comprises:

the main body is provided with a liquid cavity, the upper end of the liquid cavity is opened and is communicated with the accommodating cavity, and the lower end of the liquid cavity is provided with a first liquid outlet;

the liquid outlet head is connected to the lower end of the main body and is provided with a liquid outlet channel penetrating through the liquid outlet head;

the elastic membrane, the elastic membrane set up in go out between liquid head and the main part, the second liquid outlet has been seted up to the elastic membrane, the second liquid outlet with first liquid outlet dislocation set, the elastic membrane can switch between liquid position and backstop position.

Optionally, the diameter of the liquid inlet port of the liquid outlet channel in contact with the elastic membrane is larger than the diameter of the liquid outlet port of the liquid outlet channel, and the first liquid outlet is located in the range of the elastic membrane in contact with the liquid inlet port of the liquid outlet channel.

Another object of the present invention is to provide a microfluidic system for storing and releasing reagents.

To achieve the purpose, the second aspect of the present invention adopts the following technical solutions:

a microfluidic system comprising the multi-component storage and release device.

It is from top to bottom visible, the utility model provides a technical scheme, multicomponent save with the release kit in can assemble as required to form different reagents and deposit the combination, satisfy different kinds of micro-fluidic chip test demand, avoid appearing multicomponent save with the unmatched situation of release and micro-fluidic chip. The accommodating cavity can accommodate reagents required by multiple tests, and the reagents with preset amounts can be released as required before the tests at each time, so that the waste of the residual of the pre-buried single test reagent is avoided, the utilization rate of the reagents is improved, the waste of the reagents is reduced, and the cost of the microfluidic chip is reduced.

Drawings

FIG. 1 is a schematic diagram of a multi-component storage and release device according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a multi-component storage and delivery apparatus provided with a kit according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a slot according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a slot according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of another slot according to an embodiment of the present invention;

fig. 6 is a schematic structural diagram of another slot according to an embodiment of the present invention;

FIG. 7 is a cross-sectional view of another multiple component storage and release device provided in accordance with an embodiment of the present invention;

fig. 8 is a cross-sectional view of an elastic membrane of a control valve in a stop position according to an embodiment of the present invention;

fig. 9 is a cross-sectional view of the elastic membrane of the control valve in the liquid outlet position according to the embodiment of the present invention;

fig. 10 is a cross-sectional view of another control valve provided in an embodiment of the present invention.

In the figure:

1. a kit; 11. an accommodating chamber; 12. a slot; 121. an open end; 122. a bottom; 13. a plug-in part; 131. a root portion; 132. an end portion; 14. a limiting part;

2. a control valve; 21. a liquid outlet head; 211. a liquid outlet channel; 22. an elastic film; 221. a second liquid outlet; 23. a main body; 231. a liquid chamber; 232. a first liquid outlet.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some but not all of the elements related to the present invention are shown in the drawings.

The present invention is limited to certain orientation words, and the use of orientation words such as "upper", "lower", "left", "right", "inner" and "outer" in the case where no opposite explanation is made is convenient for understanding, and thus does not limit the scope of the present invention.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through the use of two elements or the interaction of two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The embodiment provides a multi-component storage and release device, which is used for storing and releasing a liquid reagent to provide the reagent for a microfluidic chip in multiple tests, but is not limited to this, and can also be used for providing the liquid reagent for other units to improve the utilization rate of the reagent and reduce the waste of the reagent.

As shown in fig. 1 and 2, the present embodiment provides a multicomponent preservation and release device including a cartridge 1 and a control valve 2. The kit 1 is internally provided with a containing cavity 11 for containing a reagent, the side surface of the kit 1 is provided with a slot 12 and/or an inserting part 13, and the inserting part 13 of one kit 1 can be inserted into the slot 12 of the other kit 1. A plurality of reagent cartridges 1 are inserted as necessary to provide a desired number of containing chambers 11.

It is understood that only slots 12 or only sockets 13 may be provided on one reagent vessel 1, wherein either slots 12 or sockets 13 may be provided on only one side, or slots 12 or sockets 13 may be provided on at least two sides, or sockets 13 may be provided on at least two sides. Of course, it is also possible to provide the insertion slot 12 on at least one side surface of one reagent cartridge 1 and the insertion portion 13 on at least one side surface. When the reagent kit 1 is provided with the slot 12 and the inserting part 13, the slot 12 and the inserting part 13 can be arranged on the same side of the reagent kit 1, and the slot 12 and the inserting part 13 on the two reagent kits 1 are inserted into each other. Of course, the slots 12 and the insertion parts 13 can also be arranged on different sides of the reagent vessels 1, and the insertion part 13 on one reagent vessel 1 is inserted into the slot 12 of another reagent vessel 1. The insertion grooves 12 and the insertion portions 13 may be provided on opposite sides of the reagent cartridge 1, or may be provided on adjacent sides.

Optionally, a plurality of slots 12 may be disposed on the same side surface, and the insertion portion 13 of another reagent kit 1 corresponds to the slots 12 one to one, so as to improve the stability of insertion of the reagent kit 1.

The kit 1 in the multi-component storing and releasing device provided by the embodiment can be assembled as required to form different reagent storing combinations, so that the testing requirements of different types of microfluidic chips are met, and the condition that the multi-component storing and releasing device is not matched with the microfluidic chip is avoided. The accommodating cavity 11 can accommodate reagents required by multiple tests, and a preset amount of reagents can be released as required before each test, so that the waste of the allowance of pre-embedded single test reagents is avoided, the reagent utilization rate is improved, the waste of the reagents is reduced, and the cost of the microfluidic chip is reduced.

As shown in fig. 2, optionally, the reagent kit 1 is provided with a plurality of accommodating cavities 11 arranged at intervals, for example, two accommodating cavities 11, three accommodating cavities 11, or more than three accommodating cavities 11 are provided on one reagent kit 1, and of course, in other alternative embodiments, only one accommodating cavity 11 may be provided on one reagent kit 1.

As shown in fig. 2, optionally, a position-limiting part 14 is disposed in the slot 12, and the position-limiting part 14 can be at a final position in the slot 12, so as to position the reagent cartridges 1 after the two reagent cartridges 1 are inserted.

Optionally, the lower end of the slot 12 does not penetrate the lower end of the reagent cartridge 1, so as to form a stopper 14 at the lower end of the slot 12. That is, after the inserting portion 13 is inserted into the slot 12 by a certain height, the inserting portion 13 abuts against the limiting portion 14 and cannot move downwards continuously, and the inserting portion 13 is inserted in place.

As shown in fig. 3-7, in order to prevent the insertion part 13 from being separated from the slot 12 in the depth direction of the slot 12, optionally, the size of the open end 121 of the cross section of the slot 12 is L1, the size of the bottom 122 thereof is L2, the size of the root 131 of the cross section of the insertion part 13 is L3, the size of the end 132 thereof is L4, and L3 < L1 < L4 < L2. It will be appreciated that the root 131 is the end of the spigot 13 which is connected to the cartridge 1, the root 131 being opposite the end 132, the end 132 of the cross-section of the spigot 13 being opposite the root 131. The above-described structure of the insertion part 13 and the insertion groove 12 can prevent the insertion part 13 from being separated from the insertion groove 12 in the depth direction of the insertion groove 12, that is, the arrangement direction of the open end 121 and the bottom part 122. Optionally, an insertion end 123 of the slot 12 penetrates through the upper end of the reagent cartridge 1, and the insertion end 123 is opposite to the limiting part 14. The insertion end of the insertion slot 12 penetrates the upper end of the reagent kit 1, so that the insertion part 13 is inserted into the insertion slot 12 from the insertion end of the insertion slot 12.

Optionally, the cross sections of the slot 12 and the insertion part 13 are both trapezoidal as shown in fig. 3 and 4 or "convex" as shown in fig. 5 and 6, and the slot 12 and the insertion part 13 of the above two structures can realize stable insertion.

Optionally, the reagent kit 1 is a prism, for example, the reagent kit 1 is a triangular prism, or a quadrangular prism shown in fig. 2, or a pentagonal prism or other polygonal prisms shown in fig. 7, in order to facilitate combining a plurality of reagent kits 1 as required, at least two side surfaces are each provided with a slot 12, and a user can select the slot 12 of one side surface to be plugged with the plugging portion 13 of another reagent kit 1 according to the arrangement condition of the reagent interfaces on the microfluidic chip. Or at least two side surfaces are provided with the plugging parts 13, and a user can select the plugging part 13 of one side surface to be plugged with the slot 12 of the other reagent kit 1 according to the arrangement condition of the reagent interfaces on the microfluidic chip, namely, the user can combine the reagent kits 1 according to the positions of the reagent interfaces for receiving the reagents.

Preferably, the multicomponent retention and release device may further comprise a pushing mechanism, the pushing mechanism may be hermetically connected to the upper ends of the receiving chambers 11, the pushing mechanism may be a syringe pump, a pneumatic pump or a peristaltic pump, and each receiving chamber 11 may be provided with the pushing mechanism.

The pushing mechanism is connected to the upper end of the accommodating chamber 11 and is used for pressurizing the reagent in the accommodating chamber 11. The control valve 2 is connected to the lower end of the accommodating chamber 11 so that the control valve 2 is opened to release the reagent in the accommodating chamber 11 when the pushing mechanism applies pressure to the reagent, and the control valve 2 is closed to stop releasing the reagent in the accommodating chamber 11 after the pushing mechanism stops working.

As shown in fig. 8-10, the control valve 2 includes a main body 23, a liquid outlet head 21 and an elastic membrane 22, the main body 23 is provided with a liquid cavity 231, an upper end of the liquid cavity 231 is open and is communicated with the accommodating cavity 11, and a lower end of the liquid cavity 231 is provided with a first liquid outlet 232. The liquid outlet head 21 is connected to the lower end of the main body 23, and the liquid outlet head 21 is provided with a liquid outlet channel 211 penetrating through the liquid outlet head. Optionally, the body 23 is integrally formed with the cartridge 1 to reduce the cost of the multi-component storage and release device and to avoid leakage between the body 23 and the cartridge 1.

The elastic membrane 22 is disposed between the liquid outlet head 21 and the main body 23, the elastic membrane 22 is provided with a second liquid outlet 221, the second liquid outlet 221 and the first liquid outlet 232 are arranged in a staggered manner, and the elastic membrane 22 can be switched between a liquid outlet position and a stop position.

Specifically, as shown in fig. 8, when the pushing mechanism is not operated, the elastic membrane 22 is in a natural state, the elastic membrane 22 is attached to the lower end surface of the main body 23 and is in the stop position, the elastic membrane 22 seals the first liquid outlet 232, and the reagent in the liquid chamber 231 cannot enter the liquid outlet channel 211 from the first liquid outlet 232. When the pushing mechanism works, pressure is applied to the reagent in the accommodating cavity 11, the elastic membrane 22 bends downward under the action of the pressure and is located at a liquid outlet position, a space is formed between the elastic membrane 22 and the main body 23, the reagent in the liquid cavity 231 flows into the space between the elastic membrane 22 and the main body 23 through the first liquid outlet 232, then enters the liquid outlet channel 211 through the second liquid outlet 221, and finally the reagent is released from the liquid outlet channel 211.

As shown in fig. 10, the diameter of the liquid inlet port of the liquid outlet channel 211 contacting the elastic membrane 22 is larger than the diameter of the liquid outlet port of the liquid outlet channel 211, and the first liquid outlet 232 is located in the range of the elastic membrane 22 contacting the liquid inlet port of the liquid outlet channel 211, so as to facilitate the outflow of the reagent.

In other alternative embodiments, it is understood that the inlet port of the outlet channel 211 has a certain depth in the outlet direction, so as to provide a moving space for the elastic membrane 22 to switch between the outlet position and the stop position.

Alternatively, the elastic membrane 22 may be made of a flexible material such as silicone. The elastic membrane 22 may be bonded or heat pressed to the body 23 and/or the outlet head 21. In other alternative embodiments, the main body 23 and the liquid outlet head 21 may be connected by a connecting member such as a bolt, and the elastic membrane 22 is compressively sandwiched between the main body 23 and the liquid outlet head 21, so as to avoid a gap between the elastic membrane 22 and the main body 23 or the liquid outlet head 21.

In other alternative embodiments, as shown in fig. 10, a groove may be formed in the liquid outlet head 21, and the elastic membrane 22 is disposed in the groove, so as to facilitate installation of the elastic membrane 22.

The present embodiment also provides a microfluidic system comprising the above multicomponent storage and release device. The microfluidic system may further comprise a microfluidic chip comprising a reagent interface into which the control valve 2 of the multi-component holding and releasing device is at least partially insertable so as to drop the reagent in the receiving chamber 11 into the reagent interface.

The micro-fluidic chip can also comprise a temporary storage cavity, a reaction cavity, a pump and a valve, wherein the temporary storage cavity is respectively communicated with the reagent interface and the reaction cavity, and temporarily stores the reagent released by the multi-component storing and releasing device. The valve sets up between chamber and the reagent interface of keeping in, and the break-make between chamber and the reagent interface is kept in the control, and the pump can be with the reagent pump income to the reaction chamber of keeping in the intracavity, and the reagent volume in the reaction chamber can be controlled to pump and valve. The structure of the microfluidic chip is not limited to this, as long as it can receive a reagent and obtain a quantitative amount of the reagent for testing. Since the specific structure of the microfluidic chip is the prior art, and it is not the invention of the present invention, it is not described herein again.

When the microfluidic system is used, the kit 1 is combined according to the types of reagents and the arrangement condition of the reagent interfaces, the liquid outlet head 21 of the combined multi-component storage and release device is inserted into the reagent interfaces, and the reagents are respectively dripped into the accommodating cavities 11. The pushing mechanism works to temporarily store a preset amount of reagent in the temporary storage cavity; the pump works to quantitatively convey the reagent in the temporary storage cavity to the reaction cavity. Of course, in other alternative embodiments, the operation of the microfluidic system is not so limited. The multicomponent is preserved and release device filling that this embodiment provided is simple, the production of being convenient for.

Although the invention has been described in detail in the foregoing by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that certain modifications and improvements may be made thereto based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A multi-component holding and releasing device, comprising:

the kit comprises a kit body (1), wherein a containing cavity (11) is arranged in the kit body (1), the kit body (1) comprises a side surface provided with a slot (12) and/or a plugging part (13), and the plugging part (13) of one kit body (1) can be plugged into the slot (12) of the other kit body (1);

a control valve (2), wherein the control valve (2) is connected to the lower end of the containing cavity (11).

2. Multicomponent holding and releasing device according to claim 1, wherein a stop portion (14) is arranged in the receptacle (12), the stop portion (14) being capable of defining the final position of the plug portion (13) in the receptacle (12).

3. A multicomponent holding and releasing device according to claim 2, wherein the cross-section of the insertion groove (12) has an open end (121) with a dimension L1, a base (122) with a dimension L2, the cross-section of the spigot (13) has a root (131) with a dimension L3, and an end (132) with a dimension L4, L3 < L1 < L4 < L2.

4. A multicomponent storage and release device according to claim 3, wherein the cross-sections of the receptacle (12) and the spigot (13) are trapezoidal or "crowned".

5. A multicomponent store and release device according to any one of claims 1 to 4, wherein the cartridge (1) is provided with a plurality of spaced apart receiving chambers (11).

6. The multicomponent preservation and release device according to claim 1, characterized in that the kit (1) is a prism;

the slots (12) are formed in at least two side faces; or

The insertion parts (13) are arranged on at least two side surfaces.

7. Multicomponent preservation and release device according to claim 1, characterized in that it further comprises a pushing mechanism that can be connected hermetically to the upper end of the containment chamber (11); the pushing mechanism is an injection pump, a pneumatic pump or a peristaltic pump.

8. A multicomponent preserving and releasing device according to claim 1, wherein the control valve (2) comprises:

the main body (23) is provided with a liquid cavity (231), the upper end of the liquid cavity (231) is opened and is communicated with the accommodating cavity (11), and the lower end of the liquid cavity (231) is provided with a first liquid outlet (232);

the liquid outlet head (21) is connected to the lower end of the main body (23), and a liquid outlet channel (211) penetrating through the liquid outlet head (21) is formed in the liquid outlet head (21);

elastic membrane (22), elastic membrane (22) set up in go out between liquid head (21) and main part (23), second liquid outlet (221) have been seted up in elastic membrane (22), second liquid outlet (221) with first liquid outlet (232) dislocation set, elastic membrane (22) can be switched between a liquid position and backstop position.

9. Multicomponent conservation and release device according to claim 8, wherein the outlet channel (211) has an inlet port in contact with the elastic membrane (22) with a larger diameter than the outlet port of the outlet channel (211), and the first outlet opening (232) is located within the elastic membrane (22) in contact with the inlet port of the outlet channel (211).

10. A microfluidic system comprising the multi-component storage and release device of any of claims 1-9.