CN218372289U - Nucleic acid detection kit - Google Patents

Abstract

The application relates to a nucleic acid detection kit. The nucleic acid detection kit comprises: the reagent box comprises an upper seat and a reagent box base, wherein the upper seat and the reagent box base are arranged at intervals, the upper seat and the reagent box base are in sealed connection through a sealing structure, and a sealing cavity is formed between the upper seat and the reagent box base; the upper seat is provided with a metal rod, the metal rod is arranged in the sealing cavity and is used for being magnetically connected with a magnet outside the sealing cavity; the reagent box base is provided with a plurality of containing cavities, the upper seat and the metal rod are of an integrally formed structure, and the metal rod extends towards the direction close to one side of the reagent box base from the upper seat. The nucleic acid detection kit integrates nucleic acid extraction, reagents, corresponding functional containers, amplification reaction and the like into a closed space, and can effectively avoid the problem of cross contamination in the processes of nucleic acid extraction, transfer, amplification reaction and the like.

Description

Nucleic acid detection kit

Technical Field

The application relates to the technical field of medical equipment, in particular to a nucleic acid detection kit.

Background

The magnetic bead method nucleic acid extraction kit plays an important role in the nucleic acid detection process and is one of indispensable tools in the nucleic acid extraction and detection processes. The principle is that the magnetic beads are special nano-micron materials, the size of the magnetic beads is usually between a few tenths of microns and a few microns, the magnetic beads can rapidly move to one side in a magnetic field and are gathered together, the magnetic beads can rapidly recover to a dispersion state after the magnetic field is removed, the surfaces of the magnetic beads for extracting nucleic acid have specific properties, the nucleic acid can be adsorbed on the surfaces under certain conditions, and the nucleic acid can be released from the surfaces under other conditions for subsequent detection steps. The traditional nucleic acid analysis has the defects of long time consumption, large equipment volume, need of special experimental environment and personnel and the like, and because of the ultrahigh sensitivity of nucleic acid amplification, the nucleic acid amplification is carried out in the open environment of a common laboratory, and aerosol cross contamination of a sample can easily cause false positive of nucleic acid detection of a subsequent sample. The existing nucleic acid detection box is difficult to combine the whole processes of nucleic acid extraction and nucleic acid detection into the same closed space, so that aerosol cross contamination of a sample caused by the need of separating the whole processes of nucleic acid extraction and nucleic acid detection can easily cause false positive of nucleic acid detection of a subsequent sample in the nucleic acid detection process, and the detection efficiency is low.

SUMMERY OF THE UTILITY MODEL

For solving or partly solve the problem that exists among the correlation technique, this application provides a nucleic acid detect reagent box, has better sealing performance, can prevent cross contamination, and is small, can be used to realize that nucleic acid analysis is automatic, can effectively promote nucleic acid detect's detection efficiency.

The present application provides a nucleic acid detection kit comprising: the reagent box comprises an upper seat and a reagent box base, wherein the upper seat and the reagent box base are arranged at intervals, the upper seat and the reagent box base are in sealed connection through a sealing structure, and a sealing cavity is formed between the upper seat and the reagent box base;

the upper seat is provided with a metal rod, the metal rod is arranged in the sealing cavity and is used for being magnetically connected with a magnet outside the sealing cavity;

the reagent box base is provided with a plurality of holding cavities, at least one holding cavity is used for holding magnetic beads, the holding cavities can be communicated with the sealing cavity respectively, and the metal rod is used for transferring the magnetic beads among the holding cavities.

In one embodiment, the upper seat and the metal rod are of an integrally formed structure, and the metal rod extends from the upper seat towards a direction close to one side of the reagent box base.

In one embodiment, the plurality of accommodating cavities are respectively provided with an opening facing the sealing cavity, and the openings of the plurality of accommodating cavities are provided with sealing films used for packaging the plurality of accommodating cavities.

In one embodiment, the metal rod comprises a base and an end, the base is connected with the upper seat, the end is far away from the base, and the base is provided with a groove; and/or

The end part is provided with a puncturing part for puncturing the sealing film.

In one embodiment, the sealing structure includes a sealing ring disposed between the upper seat and the reagent box base, and the sealing ring is connected to the upper seat and the reagent box base in a sealing manner.

In one embodiment, the sealing structure comprises a sealing film arranged between the upper seat and the sealing retainer ring, the upper seat and the sealing retainer ring are arranged at intervals, and the sealing film is respectively connected with the upper seat and the sealing retainer ring in a sealing manner and forms the sealing cavity.

In one embodiment, the sealing structure comprises a sealing ring arranged between the sealing retainer ring and the reagent box base, the sealing retainer ring and/or the reagent box base are respectively provided with a sealing groove, and the sealing ring is arranged in the sealing groove and is in contact with the sealing retainer ring and the reagent box base;

the sealing retainer ring and the kit base are respectively provided with connecting surfaces which are mutually contacted and used for forming a sealing state.

In one embodiment, the nucleic acid detection kit comprises a pre-buried tube for accommodating reactants;

the embedded pipe comprises a cylinder body and aluminum foil films arranged at two ends of the cylinder body, and the aluminum foil films are used for sealing two ends of the cylinder body;

and a step structure is formed on the wall of at least one accommodating cavity, one end of the embedded pipe is matched and connected with the step structure, and the step structure is sealed through the aluminum foil film.

The technical scheme provided by the application can comprise the following beneficial efficiencies:

the application provides a nucleic acid detection kit, through seal structure sealing connection between upper seat and the reagent box base, and be formed with sealed chamber between upper seat and reagent box base, sealed intracavity is located to the metal rod of upper seat, through being connected the metal rod with the outside magnet magnetism in sealed chamber, make the metal rod have magnetism, make the metal rod can shift between a plurality of chambeies that hold of magnetic bead on the reagent box base, so, the sealed environment of nucleic acid detection kit has been guaranteed, reduce sample cross contamination's risk, can effectively promote nucleic acid detection's detection efficiency.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.

Drawings

The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application, as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.

FIG. 1 is a schematic diagram of the overall structure of a nucleic acid detection kit according to an embodiment of the present application;

FIG. 2 is a schematic diagram of an exploded structure of a nucleic acid detection kit according to an embodiment of the present application;

FIG. 3 is a schematic diagram of an exploded structure of a nucleic acid detection kit according to another embodiment of the present application;

FIG. 4 is a schematic structural diagram of a metal rod of a nucleic acid detection kit according to another embodiment of the present application;

FIG. 5 is a schematic structural diagram of an upper seat of a nucleic acid detection kit according to an embodiment of the present application;

FIG. 6 is a schematic diagram of a kit base of a nucleic acid detection kit according to an embodiment of the present application;

FIG. 7 is a schematic diagram of the structure of a kit base of a nucleic acid detection kit according to another embodiment of the present application;

FIG. 8 is a schematic structural diagram of an assembled upper seat and a kit base of the nucleic acid detection kit according to an embodiment of the present application;

fig. 9 is a schematic structural diagram of a pre-buried tube of the nucleic acid detection kit according to an embodiment of the present application.

Reference numerals:

100. an upper seat; 110. a metal rod; 111. a puncture section; 1101. a placement chamber; 1102. a trench; 120. positioning the surface; 102. a clamping hole; 130. a first seal member; 103. sealing the detection hole; 104. a fixture positioning guide groove; 105. a manipulator clamping groove; 200. a reagent cartridge base; 201. an accommodating chamber; 202. a sample addition port; 203. a storage hole; 210. sealing the film; 220. a second seal member; 230. a step structure; 301. sealing the cavity; 401. a sealing surface; 410. a sealing retainer ring; 402. a sealing groove; 420. a sealing film; 403. a connecting surface; 430. a seal ring; 510. a guide ring; 600. a liquid transferring gun; 700. a check valve; 800. a limiting ring; 900. pre-burying a pipe; 910. a barrel; 920. an aluminum foil film.

Detailed Description

Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are illustrated in the accompanying drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present application. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

In the description of the present application, it is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the system or element being referred to must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and therefore, should not be considered as limiting.

Unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are intended to be inclusive and mean that, for example, they may be fixedly connected or detachably connected or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In the related art, the sealing performance of the magnetic bead method nucleic acid detection kit is poor, so that the detection efficiency of an experimenter when the magnetic bead method nucleic acid detection kit is used for detecting nucleic acid is poor. In order to solve or partially solve the problems existing in the related art, the application provides a nucleic acid detection kit, which has better sealing performance and can effectively improve the detection efficiency of nucleic acid detection.

The technical solutions of the embodiments of the present application are described in detail below with reference to the accompanying drawings.

Referring to fig. 1-3 together, the nucleic acid detecting kit of the present embodiment includes: the reagent kit comprises an upper seat 100 and a reagent kit base 200, wherein the upper seat 100 and the reagent kit base 200 are arranged at intervals, the upper seat 100 and the reagent kit base 200 are hermetically connected through a sealing structure, and a sealing cavity 301 is formed between the upper seat 100 and the reagent kit base 200; the upper seat 100 is provided with a metal rod 110, the metal rod 110 is arranged in the sealed cavity 301, and the metal rod 110 is used for being magnetically connected with a magnet outside the sealed cavity 301; the reagent box base 200 is provided with a plurality of holding cavities 201, at least one holding cavity 201 is used for holding magnetic beads, the plurality of holding cavities 201 can be respectively communicated with the sealing cavity 301, and the metal rod is used for transferring the magnetic beads among the plurality of holding cavities 201.

It can be seen from this embodiment that, the upper seat 100 is connected with the reagent kit base 200 in a sealing manner through a sealing structure, and a sealing cavity 301 is formed between the upper seat 100 and the reagent kit base 200, the metal rod 110 on the upper seat 100 is arranged in the sealing cavity 301, and the metal rod 110 is magnetically connected with a magnet outside the sealing cavity 301, so that the metal rod 110 has magnetism, and magnetic beads can be transferred between the plurality of accommodating cavities 201 on the reagent kit base 200 by the metal rod 110, thereby ensuring the sealing environment of the nucleic acid detection reagent kit, reducing the risk of sample contamination, and effectively improving the detection efficiency of nucleic acid detection. The traditional nucleic acid analysis has the defects of long time consumption, large equipment volume, need of special experimental environment and personnel and the like, and because of the ultrahigh sensitivity of nucleic acid amplification, the nucleic acid amplification is carried out in the open environment of a common laboratory, and aerosol cross contamination of a sample easily causes false positive of nucleic acid detection of a subsequent sample. The nucleic acid detection kit can perform processes such as nucleic acid extraction and amplification without cross contamination in a fully-closed container, can perform processes such as nucleic acid extraction and amplification reaction in a fully-closed space, has better sealing performance, can prevent cross contamination, is small in size, and can be used for realizing automation of nucleic acid analysis.

It should be noted that, the plurality of accommodating cavities 201 may include a lysis cavity, a cleaning cavity, a magnetic bead or magnetic bead liquid storage cavity, and a PCR (polymerase chain reaction) reaction cavity, wherein the PCR reaction cavity may be disposed on an independent PCR tube, and then the PCR tube is hermetically connected to the kit base 200, and the PCR reaction cavity and the other accommodating cavities 201 may also be formed by forming modes such as punch forming, die-casting forming, etc. on the kit base 200. In some embodiments, the PCR reaction tube has a thickness of 0.1mm to 1.5mm, and specifically may be 0.4mm, 0.8mm, 1.0mm or 1.5mm, and has a light transmittance of 60% or more, so as to pass through optical detection.

In some embodiments, the plurality of accommodating cavities 201 are respectively provided with an opening facing the sealing cavity 301, in order to ensure the sealing efficiency of the plurality of accommodating cavities 201, the sealing films 210 are arranged at the openings of the plurality of accommodating cavities 201, and the sealing films 210 are used for encapsulating the plurality of accommodating cavities 201, it should be noted that the plurality of accommodating cavities 201 may be encapsulated by using one whole sealing film 210, or the plurality of sealing films 210 may be used for respectively encapsulating the plurality of accommodating cavities 201. Preferably, the sealing film 210 is an aluminum foil film.

In some embodiments, the shape of the sealing film 210 corresponds to the arrangement shape of the openings of the plurality of accommodating cavities 201, preferably, the openings of the plurality of accommodating cavities 201 are arranged on the reagent kit base 200 along a U-shaped structure, and the shape of the sealing film 210 corresponds to a U-shape.

In some embodiments, the amount of magnetic force conducted by the metal rod 110 is varied by adjusting the distance between the magnet outside the sealed chamber 301 and the metal rod 110. Illustratively, the magnetic beads can be adsorbed by the metal rod 110 by making the magnet close to the metal rod 110 so that the magnetism of the metal rod 110 is enhanced, and the magnetic beads adsorbed by the metal rod 110 can be dropped by making the magnet far from the metal rod 110 so that the magnetism of the metal rod 110 is weakened, so that the magnetic beads can be transferred among the plurality of accommodating cavities 201.

In some embodiments, in order to ensure the sealing efficiency of the upper seat 100 and reduce the production processes of the upper seat 100, the upper seat 100 and the metal rod 110 are integrally formed, and the metal rod 110 is inserted into the upper seat 100 during the injection molding production, so that the metal rod 110 is embedded into the upper seat 110 and integrally formed with the upper seat 110. The metal bar 110 extends from the upper seat 100 toward a side close to the cartridge base 200 so as to be close to the accommodation chamber 201 on the cartridge base 200.

Referring to fig. 4, in some embodiments, the metal rod 110 includes a base connected to the upper base 100 and an end away from the base, in order to enable the metal rod 110 to adsorb and transfer magnetic beads in the accommodating chamber 201, the end of the metal rod 110 is provided with a puncturing portion 111 for puncturing the sealing film 210, so that the accommodating chamber 201 can communicate with the sealing chamber 301, so that the metal rod 110 can adsorb and transfer magnetic beads in the accommodating chamber 201. In some embodiments, the base portion is provided with a groove 1102, so that the base portion of the metal rod 110 can be embedded into the upper injection molding part more firmly, and the metal rod 110 can not loosen and generate air leakage when penetrating the sealing film 210.

Referring to fig. 5 and 6, in some embodiments, in order to make the upper base 100 meet the requirement of industrial production, the fixture can accurately clamp the upper base 100, and the upper base 100 is provided with an arc-shaped positioning surface 120 for positioning the movement of the fixture. In order to facilitate the clamp to stably clamp the upper seat 100, the upper seat 100 is provided with clamping holes 102, and preferably, the opposite sides of the upper seat 100 are respectively provided with the clamping holes 102 to improve the clamping stability of the clamp. In other embodiments, the upper housing 100 is provided with a clamp positioning guide groove 104 and a robot clamping groove 105, the clamp positioning guide groove 104 is disposed adjacent to the robot clamping groove 105, and the clamp positioning guide groove 104 has a wedge-shaped guide surface so as to guide a clamp along the guide surface to the robot clamping groove 105, thereby clamping the upper housing 100. Preferably, the jig positioning guide groove 104 and the robot clamping groove 105 are annular structures so that the jig can clamp the upper seat 100 from any angle.

In some embodiments, in order to ensure the sealing quality of the upper seat 100 and/or the assembly formed by assembling the upper seat 100 and other components, it is necessary to perform sealing detection on the upper seat 100 and/or the assembly formed by assembling the upper seat 100 and other components, the upper seat 100 is provided with a sealing detection hole 103 and a first sealing element 130, the sealing detection hole 103 is communicated with the sealing cavity 301, and the first sealing element 130 is movably connected with the sealing detection hole 103 for sealing the sealing detection hole 103. In this embodiment, the sealing detection hole 103 may also be referred to as a leak detection hole, and the first sealing member 130 may be a sealing plug.

In some embodiments, the sealing structure includes a gasket 410 disposed between the upper housing 100 and the cassette base 200, and the gasket 410 is sealingly coupled to the upper housing 100 and the cassette base 200, respectively. Thus, by providing the sealing retainer 410 between the upper seat 100 and the reagent cartridge base 200, the sealing retainer 410 can respectively connect the upper seat 100 with the reagent cartridge base 200, which have a large difference in shape and structure, in a sealing manner, thereby ensuring the sealing quality between the upper seat 100 and the reagent cartridge base 200.

In some embodiments, the sealing structure includes a sealing diaphragm 420 disposed between the upper seat 100 and the gasket 410, the upper seat 100 is spaced apart from the gasket 410, and the sealing diaphragm 420 is sealingly coupled to the upper seat 100 and the gasket 410, respectively, and forms the sealing cavity 301. In this embodiment, the sealing film 420 may be PP (polypropylene), PE (polyethylene), or a PP/PE composite film, and the sealing between the sealing film 420 and the upper seat 100 and the sealing between the sealing film 420 and the sealing ring 410 may be achieved by a heat sealing process. In some embodiments, in order to ensure the sealing efficiency between the sealing film 420 and the upper seat 100 and between the sealing film 420 and the gasket 410, the upper seat 100 and the gasket 410 respectively have a sealing surface 401 with a circular arc shape, so that the sealing film 420 can be well spread on the sealing surface 401 to perform the heat sealing operation, thereby ensuring the sealing efficiency between the sealing film 420 and the upper seat 100 and between the sealing film 420 and the gasket 410. After the seal between the seal membrane 420 and the upper seat 100, and between the seal membrane 420 and the gasket 410, is completed, a seal cavity 301 can be formed between the gasket 410 and the upper seat 100.

In some embodiments, the sealing structure comprises a sealing ring 430 disposed between the sealing ring 410 and the reagent cartridge base 200, the sealing ring 410 and/or the reagent cartridge base 200 are respectively provided with a sealing groove 402, and the sealing ring 430 is disposed in the sealing groove 402 and contacts with the sealing ring 410 and the reagent cartridge base 200; the sealing collar 410 and the cartridge base 200 each have a connecting surface 403 which is in contact with each other and which is used to establish a sealed state. In this embodiment, the sealing retainer ring 410 and the kit base 200 may be welded together by a plastic welding process, and in the plastic welding process, the sealing ring 430 in the sealing groove 402 is heated to deform and fill the sealing groove 402, so as to seal the single sealing ring and the kit base 200. In this embodiment, the sealing groove 402 may be disposed around the edge of the sealing ring 410 and/or the cartridge base 200, the connection surface 403 may also be referred to as a welding surface, and the welding surfaces may be disposed on the opposite planes between the sealing ring and the cartridge base 200, the outer side of the cartridge base 200, and the inner side of the sealing ring 410 corresponding to the outer side of the cartridge base 200.

Referring to fig. 7, in some embodiments, the reagent box base 200 is provided with a storage hole 203 for storing the metal rod 110, and the hole wall of the storage hole 203 is provided with a clamping rib, so that when the metal rod 110 is pushed into the bottom of the storage hole 203, the clamping rib can clamp and fix the metal rod 110, thereby fixing the whole upper seat 100 above the reagent box base 200.

In some embodiments, the nucleic acid detecting kit comprises a positioning structure, the positioning structure comprises a guide ring 510 disposed on one of the gasket 410 and the cassette base 200, and a guide groove disposed on the other of the gasket 410 and the cassette base 200, and the guide ring 510 is cooperatively connected with the guide groove. The positioning structure can ensure the assembly accuracy of the gasket 410 and the reagent kit base 200, and the guide ring 510 and the guide groove may be in an annular structure to ensure the concentricity of the connection of the gasket 410 and the reagent kit base 200.

In some embodiments, the reagent kit base 200 includes a plurality of platforms that hold the chamber 201, and the periphery of platform is located to the seal groove 402 ring, and the guide ring 510 is located the edge of platform and is protruding in the plane that the platform was located, and like this, after the guide ring 510 and the guide way cooperation are connected, the guide ring 510 can play certain sealed effect to the platform, and then promotes the sealed efficiency of sealed chamber 301 between reagent kit base 200 and the upper seat 100.

In some embodiments, the plurality of accommodating cavities 201 are formed in the top of the reagent cartridge base 200, the top of the reagent cartridge base 200 is a side of the reagent cartridge base 200 facing the upper seat 100, at least one sample port 202 communicated with the accommodating cavities 201 is formed in a side surface of the reagent cartridge base 200, a second sealing member 220 is movably disposed at the sample port 202, and the second sealing member 220 is used for sealing the sample port 202. In this embodiment, the accommodating chamber 201 communicated with the sample port 202 may be a lysis chamber for performing a lysis reaction, a reaction solution may be added into the lysis chamber through the sample port 202, and the second sealing member 220 may be a sealing plug. To prevent the second seal 220 from being lost, in some embodiments, the second seal 220 is connected, preferably hinged, to the cassette base 200.

In some embodiments, the nucleic acid detection kit is further used in combination with an external sample application member, wherein the sample application member can move in and out of the pipette tip 600 of the sample application port 202. The sample adding component is a standard consumable.

In other embodiments, the nucleic acid detection reagent device includes a check member disposed in the sample inlet 202, the check member includes a check valve 700 disposed in the sample inlet 202, the check valve 700 abuts against an opening wall of the sample inlet 202, the check valve 700 is provided with a liquid inlet and outlet channel, and the liquid inlet and outlet channel is used for the pipette tip 600 to pass through. The check valve 700 may have a conical structure, which can effectively prevent the sample from flowing backward. Further, in order to further prevent the backflow of the sample injected from the sample injection port 202, the sample injection port 202 is inclined from top to bottom, so that the sample injected from the pipette tip 600 can flow into the accommodating chamber 201 along the opening wall of the sample injection port 202 in an inclined manner under the action of gravity, and the backflow of the sample is effectively avoided. In some embodiments, a limiting ring 800 is arranged in the check valve 700, the limiting ring 800 is provided with an exhaust hole, and a through hole for the pipette tip 600 to pass through is formed in the center of the limiting ring 800, so that in the process of injecting the sample into the accommodating cavity 201, the gas in the accommodating cavity 201 can be discharged through the exhaust hole, and smooth addition of the sample is ensured. The pipette tip 600 may be a consumable material for sample addition, is not stored in the reagent cassette, and simply represents a state at the time of limiting the sample addition at the sample addition port.

Referring to fig. 8 and 9 together, in some embodiments, the nucleic acid detecting kit includes a pre-buried tube 900 for accommodating a reactant; the embedded pipe 900 comprises a cylinder body 910 and aluminum foil films 920 arranged at two ends of the cylinder body 910, wherein the aluminum foil films 920 are used for sealing two ends of the cylinder body 910; a step structure 230 is formed on the wall of at least one accommodating cavity 201, one end of the embedded pipe 900 is connected with the step structure 230 in a matching manner, and the step structure 230 is sealed by an aluminum foil film 920. The pre-buried pipe 900 is used for storing reactants in advance, the reactants include liquid or solid for reaction, in the process of pre-burying the reactants, the pre-buried pipe 900 firstly uses the aluminum foil film 920 to seal one end of the cylinder 910, then the reactants are loaded into the cylinder 910, and finally the aluminum foil film 920 is used to seal the other end of the cylinder 910, so that pre-burying of the reactants is completed. The pre-buried pipe 900 is generally installed in the accommodating chamber 201 for the nucleic acid amplification reaction process, and at the time of reaction, the aluminum foil film 920 at both ends of the pre-buried pipe 900 is pierced by the metal rod 110 so that the reactant in the pre-buried pipe 900 can flow into the accommodating chamber 201 to perform the nucleic acid amplification reaction process. The reagent box base 200 is concavely provided with an embedded tube cavity and a reagent cavity at the position of the step structure 230, the embedded tube 900 is placed in the embedded tube cavity, and the aluminum foil film 920 at the bottom of the embedded tube 900 is tightly pressed and sealed with the step structure 230, so that the sealing performance between the embedded tube cavity and the reagent cavity is ensured. The pre-buried pipe 900 may be used to contain reactants, and may also be used to contain reagents or other objects.

The aspects of the present application have been described in detail hereinabove with reference to the accompanying drawings. In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments. Those skilled in the art should also appreciate that the acts and modules referred to in the specification are not necessarily required in the present application. In addition, it can be understood that the steps in the method of the embodiment of the present application may be sequentially adjusted, combined, and deleted according to actual needs, and the modules in the system of the embodiment of the present application may be combined, divided, and deleted according to actual needs.

Having described embodiments of the present application, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen in order to best explain the principles of the embodiments, the practical application, or improvements made to the technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.

Claims (7)

1. A nucleic acid detection kit, comprising:

the reagent box comprises an upper seat and a reagent box base, wherein the upper seat and the reagent box base are arranged at intervals, the upper seat and the reagent box base are in sealed connection through a sealing structure, and a sealing cavity is formed between the upper seat and the reagent box base;

the upper seat is provided with a metal rod, and the metal rod is arranged in the sealing cavity;

the reagent box base is provided with a plurality of accommodating cavities, at least one accommodating cavity is used for accommodating magnetic beads, the accommodating cavities can be respectively communicated with the sealing cavity, and the metal rod is used for transferring the magnetic beads among the accommodating cavities;

the upper seat and the metal rod are of an integrally formed structure, and the metal rod extends towards the direction close to one side of the reagent box base.

2. The nucleic acid detection kit according to claim 1, characterized in that:

the containing cavities are provided with openings right facing the sealing cavities respectively, the openings of the containing cavities are provided with sealing films, and the sealing films are used for packaging the containing cavities.

3. The nucleic acid detection kit according to claim 2, characterized in that:

the metal rod comprises a base and an end part, the base is connected with the upper seat, the end part is far away from the base, and the base is provided with a groove; and/or

The end part is provided with a puncturing part for puncturing the sealing film.

4. The nucleic acid detection kit according to claim 1, characterized in that:

the sealing structure comprises a sealing retainer ring arranged between the upper seat and the reagent box base, and the sealing retainer ring is respectively connected with the upper seat and the reagent box base in a sealing way.

5. The nucleic acid detection kit according to claim 4,

the sealing structure comprises an upper seat and a sealing film between the sealing check rings, the upper seat is arranged at an interval with the sealing check rings, and the sealing film is respectively connected with the upper seat and the sealing check rings in a sealing manner and forms the sealing cavity.

6. The nucleic acid detection kit according to claim 4, characterized in that:

the sealing structure comprises a sealing ring arranged between the sealing retainer ring and the reagent box base, the sealing retainer ring and/or the reagent box base are/is respectively provided with a sealing groove, and the sealing ring is arranged in the sealing groove and is contacted with the sealing retainer ring and the reagent box base;

the sealing retainer ring and the kit base are respectively provided with connecting surfaces which are mutually contacted and used for forming a sealing state.

7. The nucleic acid detection kit according to claim 1, characterized in that:

the nucleic acid detection kit comprises a pre-buried pipe for accommodating reactants;

the embedded pipe comprises a cylinder body and aluminum foil films arranged at two ends of the cylinder body, and the aluminum foil films are used for sealing two ends of the cylinder body;

and a step structure is formed on the wall of at least one accommodating cavity, one end of the embedded pipe is matched and connected with the step structure, and the step structure is sealed through the aluminum foil film.

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