CN217561334U - Reagent card - Google Patents

Abstract

The utility model provides a reagent card, include: the sample area is arranged on the main body part and is used for communicating with a container for containing a sample to be measured; the reagent area is arranged on the main body part, a reagent mixed with a sample to be detected is arranged in the reagent area, the reagent area is provided with an inlet and an outlet, the inlet is communicated with the sample area, the reagent area comprises a rotor placing area and a reagent placing area which are independently arranged, and the reagent is arranged in the reagent placing area; the rotor is arranged in the rotor placing area. Use the technical scheme of the utility model can solve the problem that the shelf life of the reagent card among the correlation technique is short effectively.

Description

Reagent card

The application claims the application number as: 202110466981.9 filed as: the priority of the Chinese patent application entitled reagent card and liquid detection device having the same was 28/04/2021.

Technical Field

The utility model relates to a liquid detection area particularly, relates to a reagent card.

Background

Microfluidic detection is a development direction of in vitro diagnosis, food safety and pesticide residue detection at present. The reagent card is a product commonly used in the field of microfluidic detection, and is widely applied in the field of microfluidic detection due to small volume and small sampling amount. At present, in the application process of a reagent card, a collected liquid sample and a reagent are often required to be mixed, and then the property of the mixed liquid sample is detected.

In the related art, a reagent area is generally disposed in a reagent card, and a reagent to be mixed with a liquid sample is disposed in the reagent area, wherein the reagent may be in various states such as powder, solid particles, freeze-dried beads, and liquid. The liquid sample needs to be thoroughly mixed with the reagent before entering the next testing stage.

In the related art, a rotor is generally arranged in a reagent zone, and the rotor is driven to move through an external structure to achieve the effect of mixing the reagent and the blood, but because the reagent and the rotor are positioned in the same space, the reagent and the rotor may have a chemical reaction after being in long-term contact, so that the reagent is lost, and other new substances are generated. The loss of reagent can change the mixing ratio of the reagent and the liquid sample and influence the testing effect of the reagent card. After the newly generated substance is mixed with the liquid sample, the properties of the liquid sample are changed, so that the test effect of the reagent card is inaccurate. In order to ensure the accuracy of the detection of the reagent card, the shelf life of the reagent card needs to be shortened, so that the shelf life of the reagent card is too short to be transported and stored.

SUMMERY OF THE UTILITY MODEL

The main object of the present invention is to provide a reagent card, which solves the problem of short shelf life of reagent cards in the related art.

In order to achieve the above object, according to an aspect of the present invention, there is provided a reagent card including: the sample area is arranged on the main body part and is used for communicating with a container for containing a sample to be measured; the reagent area is arranged on the main body part, a reagent mixed with a sample to be detected is arranged in the reagent area, the reagent area is provided with an inlet and an outlet, the inlet is communicated with the sample area, the reagent area comprises a rotor placing area and a reagent placing area which are independently arranged, and the reagent is arranged in the reagent placing area; and the rotor is arranged in the rotor placing area.

Further, the rotor placing area is arranged around the circumferential outer side of the reagent placing area.

Further, the rotor placing area is an annular area.

Further, the reagent card further comprises: the reagent groove has been seted up to the curb plate, lid on the main part, reagent groove's cell wall and curb plate enclose into the reagent district, are provided with on the diapire in reagent district and enclose the fender structure, enclose the regional reagent that forms within the fender structure and place the district, enclose the regional rotor that forms in the fender structure circumference outside and place the district.

Further, the enclosing structure is an annular convex rib, or the enclosing structure comprises a plurality of protrusions arranged at intervals, and the distance between every two adjacent protrusions is smaller than the diameter of the rotor.

Further, the reagent card further comprises: the reagent box comprises side plates, a main body part and a rotor, wherein the side plates are covered on the main body part, a reagent groove is formed in the main body part, a reagent area is formed by the groove wall and the side plates of the reagent groove in a surrounding mode, an annular groove is formed in the bottom wall of the reagent groove, the annular groove forms a rotor placing area, and a reagent placing area is formed in the annular groove.

Further, reagent is placed and is provided with the holding tank in the district.

Further, the distance between the diapire of reagent groove and the curb plate is H1, and the maximum distance between the diapire of annular and the curb plate is H2, and the diameter H3 of rotor satisfies: h1 is more than H3 and less than H2.

Further, the difference between the maximum distance H2 between the bottom wall of the ring groove and the side plate and the distance H1 between the bottom wall of the reagent tank and the side plate is between 0.3mm and 0.5 mm.

Further, the difference between the maximum distance H2 between the bottom wall of the ring groove and the side plate and the diameter H3 of the rotor is larger than 0mm and smaller than 0.3mm.

Further, the volume of the reagent zone is between 60 and 80. Mu.L, and the volume of the holding tank is between 1 and 6. Mu.L.

Further, the main part includes relative first side and the second side that sets up, and the reagent card still includes: the first gas channel is arranged on the main body part, the first end of the first gas channel is communicated with the top of the reagent area, and the second end of the first gas channel is communicated with external gas source equipment; the first communicating channel penetrates through the first side surface and the second side surface of the main body part, and the first end of the first gas channel is communicated with the top of the reagent area through the first communicating channel; the first liquid seal structure is arranged in the first communication channel, and can disconnect the reagent area from the first gas channel when the first liquid seal structure is contacted with a sample to be detected; wherein the reagent zone is disposed on the first side and the first gas channel is disposed on the second side.

Use the technical scheme of the utility model, set up the rotor respectively in the reagent district of reagent card and place the district and the reagent is placed the district. A reagent mixed with the blood sample is placed in the reagent placement area, and a rotor used in cooperation with an external device is placed in the rotor placement area. Because the reagent placing area and the rotor placing area are arranged independently, the probability of the rotor contacting with the reagent is extremely low in the transportation and storage processes of the reagent card, so that the probability of the rotor chemically reacting with the reagent is extremely low, and the quality guarantee period of the reagent card is effectively prolonged.

Drawings

The accompanying drawings, which form a part of the present application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:

figure 1 shows a schematic perspective view of an embodiment of a reagent card according to the present invention;

FIG. 2 shows a perspective view of the body portion of the reagent card of FIG. 1;

FIG. 3 shows a front view of the body portion of the reagent card of FIG. 2;

FIG. 4 shows an enlarged schematic view of the body portion of the reagent card of FIG. 3 at A;

FIG. 5 shows a cross-sectional view of the body portion of the reagent card of FIG. 4 taken along line D-D;

FIG. 6 shows a cross-sectional view B-B of the body portion of the reagent card of FIG. 3; and

fig. 7 shows an enlarged schematic structure of the reagent card of fig. 6 at C of the body portion.

Wherein the figures include the following reference numerals:

1. a main body portion; 10. a reagent zone; 11. a reagent placement area; 111. accommodating grooves; 12. a rotor placement area; 20. a rotor; 30. a side plate; 40. a sample area; 50. a first gas passage; 60. a first communicating passage; 61. a first liquid seal structure; 70. a first liquid passage.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

Unless specifically stated otherwise, the relative arrangement of the components and steps, the numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present invention. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be discussed further in subsequent figures.

The reagent card of this application mainly used detects the phase transition process of liquid, specifically, the character change that the reagent card of this application can detect the polymer solution and solidify the in-process and produce to this coagulation effect who judges polymer solution. The polymer solution can be protein solution, protein curd, gelatin, etc. The structure and effect of the reagent card are described in the present application by taking a blood sample as an example, but the present application is not limited to the detection of a blood sample.

The coagulation of blood in a living body or a blood vessel causes thrombosis and thrombotic diseases, for example: myocardial infarction, cerebral thrombosis, venous thrombosis, disseminated intravascular coagulation and the like. Extravascular hemorrhages can lead to bleeding disorders such as: allergic purpura, thrombocytopenic purpura, hemophilia, liver disease hemorrhage, etc. In order to effectively suppress these diseases, it is necessary to periodically detect the thrombus formation in the blood of the user. The thrombus condition for a user's blood is currently generally characterized by thromboelastography. The manner of drawing the elastogram in the related art is as follows: the blood sample of a user to be detected is firstly taken, the blood sample of the user is led into a reagent card for thrombus testing, a reagent area is arranged in the reagent card, a reagent which needs to be mixed with the blood of the user is placed in the reagent area, and the blood sample of the user can enter the next detection stage after being fully mixed with the reagent.

In order to ensure the detection accuracy of the reagent card, as shown in fig. 1 to 4, the reagent card of the first embodiment includes: a body portion 1, a sample zone 40, a reagent zone 10, and a rotor 20. Wherein, the sample area 40 is arranged on the main body part 1 and is used for communicating with a container for accommodating a sample to be measured; the reagent area 10 is arranged on the main body part 1, a reagent mixed with a sample to be tested is arranged in the reagent area 10, the reagent area 10 is provided with an inlet and an outlet, the inlet is communicated with the sample area 40 through a first liquid channel 70, the reagent area 10 comprises a rotor placing area 12 and a reagent placing area 11 which are independently arranged, and the reagent is arranged in the reagent placing area 11; the rotor 20 is disposed in the rotor disposing region 12.

By applying the technical scheme of the embodiment, a rotor placing area 12 and a reagent placing area 11 are respectively arranged in a reagent area 10 of a reagent card. A reagent mixed with a blood sample is placed in the reagent placing section 11, and a rotor used in cooperation with an external device is placed in the rotor placing section 12. Because reagent placing area 11 and rotor placing area 12 are independent of each other, the independent of each other means that the rotor placed in rotor placing area 12 is limited not to enter into reagent placing area 11, and the reagent placed in reagent placing area 11 is also limited not to enter into rotor placing area 12. Therefore, in the transportation and storage processes of the reagent card, the probability of the contact between the rotor 20 and the reagent is extremely low, so that the probability of the chemical reaction between the rotor 20 and the reagent is extremely low, the detection precision of the reagent card is improved, and the quality guarantee period of the reagent card is effectively prolonged.

The state of the reagent may be solid particles, solid powder, or liquid. In the case where the reagent is in the form of solid particles, the solid particles may be bound in the reagent holding section 11. In the case where the reagent is in the form of a solid powder, the powdered reagent may be fixed in the reagent holding section 11 by coating. In the case where the reagent is a liquid, the liquid reagent may be wrapped and fixed in the reagent housing zone 11 by a film material.

As shown in FIG. 4, in the first embodiment, the rotor disposition region 12 is disposed circumferentially outside the reagent disposition region 11. In the structure, after the reagent area 10 enters the blood sample, the reagent can be freely diffused in the blood sample, and the reagent placing area 11 is arranged at the middle part of the reagent area 10, so that the reagent can be freely diffused all around. The rotor disposing section 12 is disposed circumferentially outside the reagent disposing section 11 to facilitate an increase in the movable range of the rotor 20. The above structure optimizes the arrangement of the rotor placing region 12 and the reagent placing region 11, thereby increasing the mixing rate of the reagent and the blood sample.

As shown in fig. 4, in the first embodiment, the rotor placing area 12 is an annular area. The above structure can further increase the moving range of the rotor 20, thereby further increasing the mixing rate of the reagent and the blood sample.

As shown in fig. 1, in the first embodiment, the reagent card further includes a side plate 30, the side plate 30 is covered on the main body portion 1, the main body portion 1 is provided with a reagent tank, a reagent zone 10 is enclosed by a tank wall of the reagent tank and the side plate 30, a ring groove is provided on a bottom wall of the reagent tank, the ring groove forms a rotor placing zone 12, and a reagent placing zone 11 is formed inside the ring groove. In the above structure, the reagent zone 10 has a simple structure and is easy to process. The material of the main body may be PMMA, ABS, PP, or the like. The side plate can be made of a film material or a composite film material with adhesive glue on one side, such as silica gel, PET and the like. The main body part and the side plate can be bonded together through adhesive surfaces, or can be connected together through processes such as ultrasonic welding, laser welding and the like.

As shown in FIG. 4, in the first embodiment, a holding tank 111 is provided in the reagent holding section 11. In the above structure, the housing groove 111 can provide a space for placing the reagent, thereby facilitating the fixation of the reagent in the reagent placing region 11 and reducing the probability of the contact of the reagent with the rotor 20.

As shown in fig. 6 and 7, in the first embodiment, the distance between the bottom wall of the reagent tank and the side plate 30 is H1, the maximum distance between the bottom wall of the ring groove and the side plate 30 is H2, and the diameter H3 of the rotor 20 satisfies: h1 is more than H3 and less than H2. In the structure, because the distance H1 between the bottom wall of the reagent groove and the side plate 30 is smaller than the diameter H3 of the rotor 20, the rotor 20 can be limited between the bottom wall of the ring groove and the side plate 30, so that the probability of contact between the rotor 20 and the reagent is reduced, and the quality guarantee period of the reagent card is prolonged.

As shown in FIG. 7, in the first embodiment, the difference between the maximum distance H2 between the bottom wall of the ring groove and the side plate 30 and the distance H1 between the bottom wall of the reagent groove and the side plate 30 is between 0.3mm and 0.5 mm. In the above structure, if the difference between H2 and H1 is too small, the ring groove may not have a good effect of limiting the rotor 20. If the difference between H2 and H1 is too large, the thickness of the body portion 1 increases, increasing the production cost of the reagent card.

As shown in fig. 7, in the first embodiment, the difference between the maximum distance H2 between the bottom wall of the ring groove and the side plate 30 and the diameter H3 of the rotor 20 is greater than 0mm and less than 0.3mm. The above structure enables the rotor 20 to have a certain amount of movement between the bottom wall of the ring groove and the side plate 30, which facilitates the movement of the rotor 20 in the rotor placing region 12. The rotor 20 of the present application may be made of a magnetic material, or may be a metal member with a magnetized surface, and an external device may drive the rotor 20 to move in the rotor placing region 12 through electromagnetic force. Preferably, the rotor 20 of the present application is a steel ball whose surface is magnetized.

As shown in FIGS. 2 to 4, in the first embodiment, the volume of the reagent zone 10 is between 60 μ L and 80 μ L, and the volume of the accommodating groove 111 is between 1 μ L and 6 μ L. In the above configuration, when the reagent is in the form of solid particles, the larger the particles of the reagent, the easier the processing. Having a larger volume in the reagent zone 10 requires a corresponding increase in the amount of reagent placed in the reagent zone 10, thereby enabling the processing of the reagent to be facilitated.

As shown in fig. 2 and 5, in the first embodiment, the reagent card further includes: a first gas channel 50, a first liquid seal 61 and a first communication channel 60. The first gas channel 50 is arranged on the main body part 1, a first end of the first gas channel 50 is communicated with the top of the reagent zone 10, and a second end of the first gas channel 50 is communicated with external gas source equipment; a first liquid seal structure 61 is arranged between the reagent area 10 and the first gas channel 50, wherein the first liquid seal structure 61 can disconnect the reagent area 10 from the first gas channel 50 when contacting with a sample to be tested; the main body part 1 comprises a first side surface and a second side surface which are oppositely arranged, the reagent zone 10 is arranged on the first side surface, and the first gas channel 50 is arranged on the second side surface; a first communication channel 60, the first communication channel 60 penetrating the first side and the second side of the main body part 1, a first end of the first gas channel 50 communicating with the top of the reagent area 10 through the first communication channel 60, and a first liquid seal structure 61 located in the first communication channel 60.

In the above-mentioned structure, adopt in the negative pressure inhales reagent district 10 with blood sample to can guarantee through the mode of first liquid seal structure 61 separation blood sample that only can switch to the encapsulated situation after the whole discharges of the gas in reagent district 10, thereby realized the accurate ration in reagent district 10, guaranteed the accuracy nature of the reagent in reagent district 10 and blood sample's mixing ratio, promoted the detection precision of reagent card.

Specifically, the reagent zone of the reagent card communicates with an external gas supply device through the first liquid seal structure 61 and the first gas channel 50. When the external gas source device is turned on, a negative pressure is generated in the reagent zone 10 and the blood sample can flow from the sample zone 40 into the reagent zone 10. As the negative pressure continues, the level of the blood sample in the reagent zone 10 will gradually rise until the blood sample fills the entire reagent zone 10 and contacts the first liquid seal 61. The first liquid seal structure 61 has a gas permeable state and a closed state, and in the case where a part of the structure in the first liquid seal structure 61 is not in contact with liquid, the first liquid seal structure 61 can maintain the gas permeable state, and at this time, gas can permeate through the first liquid seal structure 61. When a part of the structure in the first liquid sealing structure 61 contacts with the liquid, the first liquid sealing structure 61 is transformed into a closed state, and the first liquid sealing structure 61 is impermeable to gas and liquid. Based on the properties of the first liquid sealing structure 61 itself, when the blood sample contacts the first liquid sealing structure 61, the first liquid sealing structure 61 can be transformed into a closed state, so that the blood sample cannot pass through the first liquid sealing structure 61. Since the volume of the reagent zone is a constant value that can be measured, the volume of the blood sample that enters the reagent zone is a constant value.

It should be noted that a joint communicating with a container of a container containing a sample to be measured is provided in the sample area 40, and the joint may be a structure having a piercing function such as a steel needle. The joint is connected with the main body part of the reagent card in a sealing way.

It should be noted that the first liquid sealing structure 61 can be a water-meeting self-sealing core, which can be obtained by procurement, specifically, by the method from H ₂ OStop procurement. The first liquid-tight structure 61 is capable of expanding rapidly upon contact with a liquid, so that the porous structure is closed. This is accomplished in a very short time, thus avoiding the blood sample from being drawn into the first gas channel 50 through the first liquid seal 61.

The reagent card of the second embodiment is different from the reagent card of the first embodiment in the specific structure of the reagent zone 10. Specifically, in the second embodiment, a barrier structure is provided on the bottom wall of the reagent zone 10, the area inside the annular protrusion forms the reagent placing zone 11, and the area outside the annular protrusion in the circumferential direction forms the rotor placing zone 12. In the above structure, the reagent zone 10 has a simple structure, and the contact of the reagent with the rotor can be effectively prevented. Specifically, the reagent may be provided in a structure having a fixed shape and fixed in the reagent holding section 11.

Specifically, the enclosure structure may be an annular rib, or the enclosure structure may include a plurality of protrusions arranged at intervals, and a distance between two adjacent protrusions is smaller than a diameter of the rotor 20. The structure can achieve a good stopping effect.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the orientation words such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, and in the case of not making a contrary explanation, these orientation words do not indicate and imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be interpreted as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

For ease of description, spatially relative terms such as "over … …", "over … …", "over … …", "over", etc. may be used herein to describe the spatial positional relationship of one device or feature to another device or feature as shown in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that the terms "first", "second", and the like are used to define the components, and are only used for convenience of distinguishing the corresponding components, and if not stated otherwise, the terms have no special meaning, and therefore, the scope of the present invention should not be construed as being limited.

Claims (12)

1. A reagent card, comprising:

a main body (1);

the sample area (40) is arranged on the main body part (1) and is used for communicating with a container for containing a sample to be measured;

a reagent zone (10) disposed on the body portion (1), the reagent zone (10) for mixing a sample to be tested with a reagent, the reagent zone (10) having an inlet and an outlet, the inlet communicating with the sample zone (40) through a first liquid channel (70), the reagent zone (10) comprising a rotor placement zone (12) and a reagent placement zone (11) disposed independently, the reagent being disposed within the reagent placement zone (11);

a rotor (20) disposed within the rotor placement area (12).

2. Reagent card according to claim 1, wherein the rotor deposit zone (12) is arranged circumferentially outside the reagent deposit zone (11).

3. The reagent card of claim 1, wherein the rotor placement zone (12) is an annular zone.

4. The reagent card of claim 1, further comprising:

the reagent storage device comprises a side plate (30) which covers the main body part (1), a reagent groove is formed in the main body part (1), the groove wall of the reagent groove and the side plate (30) form a reagent area (10), a surrounding structure is arranged on the bottom wall of the reagent area (10), a reagent placing area (11) is formed in the region inside the surrounding structure, and a rotor placing area (12) is formed in the region outside the circumferential direction of the surrounding structure.

5. The reagent card of claim 4, wherein the retaining structure is an annular rib or comprises a plurality of protrusions arranged at intervals, and the distance between two adjacent protrusions is smaller than the diameter of the rotor (20).

6. The reagent card of claim 1, further comprising:

curb plate (30), lid are established on main part (1), the reagent groove has been seted up on main part (1), the cell wall in reagent groove with curb plate (30) enclose into reagent district (10), be provided with the annular on the diapire in reagent groove, the annular becomes district (12) is placed to the rotor, the inside of annular forms district (11) is placed to the reagent.

7. Reagent card according to claim 6, characterized in that a receiving slot (111) is provided in the reagent placement zone (11).

8. The reagent card according to claim 6, wherein the distance between the bottom wall of the reagent tank and the side plate (30) is H1, the maximum distance between the bottom wall of the ring tank and the side plate (30) is H2, and the diameter H3 of the rotor (20) is such that: h1 is more than H3 and less than H2.

9. Reagent card according to claim 8, characterized in that the difference between the maximum distance H2 between the bottom wall of the ring groove and the side plate (30) and the distance H1 between the bottom wall of the reagent groove and the side plate (30) is between 0.3mm and 0.5 mm.

10. Reagent card according to claim 8, characterized in that the difference between the maximum distance H2 between the bottom wall of the annular groove and the side plate (30) and the diameter H3 of the rotor (20) is greater than 0mm and less than 0.3mm.

11. The reagent card of claim 7, wherein the volume of the reagent zone (10) is between 60 μ L and 80 μ L and the volume of the holding well (111) is between 1 μ L and 6 μ L.

12. Reagent card according to any of claims 1 to 11, wherein the body part (1) comprises a first and a second side arranged opposite each other, the reagent card further comprising:

the first gas channel (50) is arranged on the main body part (1), a first end of the first gas channel (50) is communicated with the top of the reagent area (10), and a second end of the first gas channel (50) is communicated with an external gas source device;

a first communication channel (60) penetrating the first side surface and the second side surface of the main body part (1), wherein a first end of the first gas channel (50) is communicated with the top of the reagent area (10) through the first communication channel (60);

a first liquid seal structure (61) arranged in the first communication channel (60), wherein the first liquid seal structure (61) can disconnect the reagent area (10) and the first gas channel (50) when being contacted with the sample to be tested;

wherein the reagent zone (10) is disposed on the first side and the first gas channel (50) is disposed on the second side.

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