CN216823420U - Visible-near infrared I/II area imaging device - Google Patents
Visible-near infrared I/II area imaging device Download PDFInfo
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- CN216823420U CN216823420U CN202122771396.8U CN202122771396U CN216823420U CN 216823420 U CN216823420 U CN 216823420U CN 202122771396 U CN202122771396 U CN 202122771396U CN 216823420 U CN216823420 U CN 216823420U
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Abstract
The utility model discloses a visible-near infrared I/II area imaging device, which comprises a light source module and an imaging module; the imaging module comprises an imaging light splitting box, a second dichroic mirror, a first filter wheel, a first objective lens, a visible imaging camera, a second filter wheel, a second objective lens and a near-infrared I/II area imaging camera; the imaging light splitting box is positioned above the illumination light splitting box. The imaging device is convenient to disassemble and easy to operate, can be used for independently carrying out visible light imaging or near infrared I/II area imaging, can also be used for simultaneously carrying out visible light and near infrared I/II area multispectral imaging, saves sample testing time, meets the testing requirements of more materials, and provides research support for the clinical application of the near infrared II area imaging.
Description
Technical Field
The utility model belongs to the technical field of optical imaging, and particularly relates to a visible-near infrared I/II area imaging device.
Background
The bio-optical imaging technology refers to a method for obtaining biological information in cells, tissues or even organisms by imaging the cells, tissues or even organisms by using an optical detection means. Through biological optical imaging, scientists can obtain a visual and clear image to analyze the characteristics and the state of specific regions of cells or organisms, even the expression, the distribution and other information of specific molecules. Near-infrared I-region fluorescence imaging has played an important role in various clinical scenarios because it exhibits better signal-to-noise ratio than visible imaging, methylene blue, fluorescein sodium, indocyanine green (ICG) and other near-infrared dyes have been approved by the food and drug administration for clinical routine use, many molecular targeted fluorescence probes have also been developed and are in active clinical evaluation, such as folate receptor alpha targeted fluorescence probe folate-FITC 13, c-MET targeted optical probe GE-13714 and epidermal growth factor receptor targeted probe cetuximab IRDye800CW15, etc., which makes the future of near-infrared fluorescence imaging in clinical use self evident.
However, clinically accepted near infrared I-region imaging (NIR-I, 700 and 900nm) still suffers from poor transmission (transmission of about 2 mm) and strong light scattering, resulting in poor spatial resolution. The near infrared region II (NIR-II, 1000-1700nm) has longer wavelength, can reduce photon absorption and scattering effect of human tissues, is beneficial to imaging deep into underlying tissues, and has larger application prospect in biological optical imaging than near infrared region I imaging. When the near-infrared II region is used for imaging, remarkable improvement on performance can be achieved, for example, the tissue penetration depth can reach the centimeter magnitude, the minimum resolution can reach the micrometer magnitude, the contrast can be greatly improved, and real-time observation can be achieved. For example, ICG exhibits significant fluorescence emission in the near-infrared region II, and background fluorescence in tissue volume is nearly eliminated, thus providing higher imaging signal-to-noise ratio compared to NIR-I imaging despite the reduced absolute intensity of fluorescence in NIR-II. However, due to the lack of a suitable imaging instrument, near-infrared II-region imaging has not been verified in a clinical environment, and it is urgently needed to design an integrated visible light and near-infrared I/II-region multispectral imaging device for comprehensively evaluating clinical application potential of near-infrared II-region fluorescence imaging, accelerating the clinical application of near-infrared II-region imaging, and simultaneously, having visible light and near-infrared I-region fluorescence imaging, and meeting more practical application requirements.
Disclosure of Invention
Aiming at the problems in the prior art, the utility model provides a visible-near infrared I/II area imaging device which can simultaneously perform visible-near infrared I/II area multispectral imaging of a multi-marker labeled sample, improve the imaging signal-to-noise ratio and meet the research and application requirements of various materials, clinical medical treatment and the like.
The technical scheme of the utility model is as follows:
a visible-near infrared I/II region imaging device comprises a light source module and an imaging module;
the light source module comprises a lighting light splitting box, a light source and a first dichroic mirror, and the lighting light splitting box is communicated with the light source; the first dichroic mirror is obliquely arranged in the illumination light splitting box;
the imaging module comprises an imaging light splitting box, a second dichroic mirror, a first filter wheel, a first objective lens, a visible imaging camera, a second filter wheel, a second objective lens and a near-infrared I/II area imaging camera;
the imaging light splitting box is positioned above the illumination light splitting box;
the imaging light splitting box is provided with a plurality of assembling holes for assembling the first filter wheel and the second filter wheel, and the first filter wheel is adjacent to the second filter wheel; the second dichroic mirror is obliquely arranged in the imaging light splitting box; the imaging light splitting box is sequentially provided with the first filter wheel, a first objective lens and a visible imaging camera; and the second filter wheel is sequentially connected with the second objective and the near-infrared I/II area imaging camera.
In the present application, the light source is used to provide excitation light.
According to an embodiment of the present invention, the first filter wheel and the second filter wheel are configured to pass light in a visible wavelength band and light in a near infrared wavelength band, respectively, and filter background interference light.
According to an embodiment of the present invention, the first objective lens and the second objective lens are used for collecting light beams to enter the visible imaging camera and the near-infrared I/II area imaging camera, respectively.
According to an embodiment of the utility model, the first filter wheel is fitted to the side of the imaging spectrometer box; and a second filter wheel is assembled above the imaging light splitting box.
According to the embodiment of the utility model, a plurality of assembling holes are formed in the illumination light splitting box, and the light source is assembled on the assembling holes.
According to an embodiment of the present invention, the light source module further includes a focus cylinder fitted on the fitting hole; the light source is connected with the focusing barrel; the focusing barrel is used for adjusting the focusing position of exciting light emitted by the light source.
According to an embodiment of the present invention, the installation angle of the first dichroic mirror is 45 °.
According to the embodiment of the utility model, the first dichroic mirror is obliquely arranged at 45 degrees in the illumination light splitting box; the first dichroic mirror is used for reflecting exciting light emitted by the light source and transmitting light emitted after the sample to be detected is excited.
According to an embodiment of the present invention, the mounting angle of the second dichroic mirror is 45 °.
According to an embodiment of the present invention, the second dichroic mirror is installed in the imaging splitting box at an angle of 45 °; the second dichroic mirror is used for reflecting light in a visible wave band and transmitting light in a near infrared I/II wave band.
According to an embodiment of the present invention, the visible imaging camera and the near-infrared I/II zone imaging camera are mounted in directions perpendicular to each other.
The utility model has the beneficial effects that:
(1) the visible-near infrared I/II area imaging device disclosed by the utility model is convenient to disassemble and easy to operate, can be used for independently carrying out visible light imaging or near infrared I/II area imaging and simultaneously carrying out visible light and near infrared I/II area multispectral imaging, saves the sample testing time, meets the testing requirements of more materials, and provides research support for the clinical application of near infrared II area imaging.
(2) According to the visible-near infrared I/II area imaging device, the first dichroic mirror and the second dichroic mirror are adopted for light splitting, visible imaging and near infrared I/II area imaging are simultaneously carried out, and the first filter mirror wheel and the second filter mirror wheel are adopted for filtering light, so that the imaging signal-to-noise ratio is improved.
Drawings
FIG. 1 is a front view of a visible-near infrared region I/II imaging device;
FIG. 2 is a cross-sectional view of a visible-near infrared region I/II imaging device;
FIG. 3 is a schematic view of the optical path of a visible-near infrared I/II imaging device;
FIG. 4 is a schematic view of a visible-near infrared I/II imaging device;
in the figure: the system comprises a lighting light splitting box 1, an imaging light splitting box 2, a second filter wheel 3, a second objective 4, a near infrared I/II area imaging camera 5, a first filter wheel 6, a first objective 7, a visible imaging camera 8, a focusing barrel 9, a light source 10, a first dichroic mirror 11 and a second dichroic mirror 12.
Detailed Description
The technical solution of the present invention will be further described in detail with reference to specific embodiments. It is to be understood that the following examples are only illustrative and explanatory of the present invention and should not be construed as limiting the scope of the present invention. All the technologies realized based on the above-mentioned contents of the present invention are covered in the protection scope of the present invention.
Unless otherwise indicated, the raw materials and reagents used in the following examples are all commercially available products or can be prepared by known methods.
The utility model provides a visible-near infrared I/II area imaging device which mainly comprises a light source module and an imaging module. As shown in fig. 1-3, the light source module includes an illumination light splitting box 1, a light source 10, a focusing barrel 9 and a first dichroic mirror 11, wherein the illumination light splitting box 1 is provided with a plurality of assembling holes for assembling the focusing barrel 9; the light source 10 is connected with the focusing barrel 9, the light source 10 is used for providing exciting light, and the focusing barrel 9 is used for adjusting the focusing position of the exciting light emitted by the light source 10; the first dichroic mirror 11 is obliquely installed in the illumination light splitting box 1 at an angle of 45 degrees, and the first dichroic mirror 11 is used for reflecting excitation light emitted by the light source 10 and transmitting light emitted after the excitation of a sample to be detected.
The imaging module comprises an imaging light splitting box 2, a second dichroic mirror 12, a first filter wheel 6, a first objective lens 7, a visible imaging camera 8, a second filter wheel 3, a second objective lens 4 and a near-infrared I/II area imaging camera 5; the imaging light splitting box 2 is positioned above the illumination light splitting box 1;
the imaging light splitting box 2 is provided with a plurality of assembling holes for assembling the first filter wheel 6 and the second filter wheel 3; the second dichroic mirror 12 is obliquely installed in the light splitting box at an angle of 45 degrees, and is used for reflecting light in a visible waveband and transmitting light in a near-infrared I/II waveband; the side of the imaging light splitting box 2 is sequentially connected with the first filter wheel 6, the first objective lens 7 and the visible imaging camera 8, the second filter wheel 3 is sequentially connected with the second objective lens 4 and the near-infrared I/II area imaging camera 5, and the second filter wheel 3 is positioned above the imaging light splitting box 2;
the first filter wheel 6 and the second filter wheel 3 are used for passing light in a visible waveband and light in a near-infrared waveband respectively and filtering background interference light, and the first objective lens 7 and the second objective lens 4 are used for collecting light beams to enter a visible imaging camera 8 and a near-infrared I/II area imaging camera 5 respectively.
Example 1
FIG. 4 is a schematic diagram of a visible-near infrared I/II imaging device according to the present invention. The device comprises a light source module and an imaging module, and can simultaneously perform visible light and near infrared I/II area multispectral imaging of a multi-marker labeled sample, so that the imaging signal-to-noise ratio is improved. The exciting light emitted by the light source 10 is focused through the focusing barrel 9, the exciting light is irradiated on the multiple marker marking samples after being reflected by the first dichroic mirror 11, the fluorescent light with different wavelengths emitted by the markers after the multiple marker marking samples are excited can penetrate through the first dichroic mirror 11 and reach the second dichroic mirror 12, the light with the visible wavelength band is reflected by the second dichroic mirror 12, the light with the visible wavelength band filtered by the first filter wheel 6 is collected through the first objective lens 7 and enters the visible imaging camera 8 for visible light imaging, the near infrared light penetrates through the second dichroic mirror 12, and the light with the near infrared wavelength band filtered by the second filter wheel 3 is collected through the second objective lens 4 and enters the near infrared I/II area imaging camera 5 for near infrared I area and II area light imaging. By integrating visible light imaging images and near-infrared I/II area light imaging images of a multi-marker labeled sample, a sample fluorescence image with a higher signal-to-noise ratio is obtained.
Example 2
FIG. 4 is a schematic diagram of a visible-near infrared I/II imaging device according to the present invention. The device comprises a light source module and an imaging module, and can be used for independently carrying out visible light imaging or near infrared I/II area imaging on a single marker marking sample. The exciting light emitted by the light source 10 is focused by the focusing tube 9, so that the exciting light is reflected by the first dichroic mirror 11 and then irradiates on the multiple marker marking samples, and the fluorescent light emitted by each marker after the single marker marking sample is excited can penetrate through the first dichroic mirror 11 and reach the second dichroic mirror 12. If the fluorescence emitted by the single marker marking sample under excitation is visible light, the fluorescence is reflected by the second dichroic mirror 12, and the light with the visible waveband, which is filtered by the first filter wheel 6, is collected by the first objective lens 7 and enters the visible imaging camera 8 for visible light imaging; if the fluorescence emitted by the single marker marking sample under excitation is near-infrared light, the fluorescence penetrates through the second dichroic mirror 12, and the light in the near-infrared waveband filtered by the second filter wheel 3 is collected by the second objective lens 4 and enters the near-infrared I/II area imaging camera 5 to perform near-infrared I/II area light imaging.
The embodiments of the present invention have been described above by way of example. However, the scope of the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement and the like made by those skilled in the art within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims (9)
1. A visible-near infrared I/II area imaging device is characterized by comprising a light source module and an imaging module;
the light source module comprises a lighting light splitting box, a light source and a first dichroic mirror, and the lighting light splitting box is communicated with the light source; the first dichroic mirror is obliquely arranged in the illumination light splitting box;
the imaging module comprises an imaging light splitting box, a second dichroic mirror, a first filter wheel, a first objective lens, a visible imaging camera, a second filter wheel, a second objective lens and a near-infrared I/II area imaging camera;
the imaging light splitting box is positioned above the illumination light splitting box;
the imaging light splitting box is provided with a plurality of assembling holes for assembling the first filter wheel and the second filter wheel, and the first filter wheel is adjacent to the second filter wheel; the second dichroic mirror is obliquely arranged in the imaging light splitting box; the imaging light splitting box is sequentially provided with the first filter wheel, a first objective lens and a visible imaging camera; and the second filter wheel is sequentially connected with the second objective and the near-infrared I/II area imaging camera.
2. The apparatus of claim 1, wherein the first filter wheel and the second filter wheel are configured to pass light in the visible band and light in the near infrared band, respectively, and filter background interference light.
3. The apparatus of claim 1, wherein the first objective lens and the second objective lens are configured to collect light beams into the visible imaging camera and the near-infrared I/II imaging camera, respectively.
4. The apparatus of claim 1, wherein the first filter wheel is mounted to a side of the imaging spectrometer box; and a second filter wheel is assembled above the imaging light splitting box.
5. The device of claim 1, wherein the illumination splitter box has a plurality of mounting holes, and the light source is mounted on the mounting holes.
6. The apparatus of claim 1, wherein the light source module further comprises a focusing barrel fitted on the fitting hole; the light source is connected with the focusing barrel; the focusing barrel is used for adjusting the focusing position of exciting light emitted by the light source.
7. The apparatus according to claim 1, wherein the installation angle of the first dichroic mirror is 45 °.
8. The apparatus according to claim 1, wherein the mounting angle of the second dichroic mirror is 45 °.
9. The apparatus of any one of claims 1-8, wherein the visible imaging camera and the near-infrared I/II imaging camera are mounted perpendicular to each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202122771396.8U CN216823420U (en) | 2021-11-12 | 2021-11-12 | Visible-near infrared I/II area imaging device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122771396.8U CN216823420U (en) | 2021-11-12 | 2021-11-12 | Visible-near infrared I/II area imaging device |
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| CN216823420U true CN216823420U (en) | 2022-06-28 |
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| CN202122771396.8U Active CN216823420U (en) | 2021-11-12 | 2021-11-12 | Visible-near infrared I/II area imaging device |
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