CN216614697U - Three-dimensional cell culture support - Google Patents
Three-dimensional cell culture support Download PDFInfo
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- CN216614697U CN216614697U CN202122985205.8U CN202122985205U CN216614697U CN 216614697 U CN216614697 U CN 216614697U CN 202122985205 U CN202122985205 U CN 202122985205U CN 216614697 U CN216614697 U CN 216614697U
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Abstract
The present invention provides a three-dimensional cell culture scaffold comprising: the temperature-sensitive expansion particles are embedded in the bracket body in a powdery form, and the vibration factors are distributed on the outer surface of the bracket body; the thick fiber phase is a multilayer thick fiber structure, two adjacent layers of the thick fiber phase are arranged in a staggered mode according to a certain angle, the thin fiber phase is independently combined with one or more surfaces of the thick fiber phase, the thick fiber phase forms a hole structure of the three-dimensional cell culture support, the thin fiber phase is uniformly distributed or intensively distributed in the hole structure, and the temperature-sensitive expansion particles have high sensitivity to temperature and can expand and pull the structure of the support body. The utility model can simulate the change of temperature and pressure in animal body, to enrich the database under dynamic cell culture.
Description
Technical Field
The utility model relates to the technical field of cell tissue culture instruments, in particular to a three-dimensional cell culture bracket.
Background
In the field of cell and tissue culture, two-dimensional (2D) culture scientists have seen their limitations since the last 70 s and have focused more on the advantages of three-dimensional (3D) culture, with an increasing amount of research currently being shifted from planar environments for cell culture to three-dimensional culture. Currently, 3D culture techniques have been widely used in academic research, although the most common of the pharmaceutical industry today is the 2D approach, for cell-based effector studies and toxicity testing. The physiological activities such as cell proliferation, differentiation and metabolism are severely influenced by the microenvironment. Currently, most of cell biology research is carried out in two-dimensional planar culture, and the planar culture and growth mode are greatly different from the three-dimensional environment in a body, so that the cell morphology, differentiation, interaction between cells and a matrix, and interaction between cells are obviously different from the behavior of the cells under the physiological condition in the body. The 3D culture can be designed to simulate the physiological environment in vivo, so that the physiological behavior of cells is closer to the actual physiological environment of the organism. With improvements in biological relevance, throughput, yield, and the like, along with the reduction in 3D culture cost, 3D culture will be more and more widely applied in regenerative medicine, basic research, and drug development, and a revolution of cells from 2D culture to 3D is taking place.
Cells in a living body are in an environment full of various mechanical stimuli, the cells are stretched, contracted, twisted and the like by skeletal motion during motion, the digestive tract wriggles during eating, the shearing force caused by body fluid flow and the like, and even a simple finger-operated motion can bring the mechanical stimuli to the cells in the living body. As is known to all, exercise is beneficial to health, in vivo, normal biomechanical stimulation is also necessary for normal physiological processes such as bone tissue balance, embryonic development and the like, and research shows that different mechanical stimulation can have great influence on life activities such as growth, proliferation, differentiation and the like of cells, and abnormal biomechanical stimulation can also generate diseases such as osteoporosis and the like. The existing three-dimensional cell culture support mechanical stimulation loading device has the problem of poor stability to the stimulation mode of cells, and the cell growth environment in an animal body cannot be truly simulated.
In view of the above, there is a need for an improved three-dimensional cell culture scaffold in the prior art to solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to disclose a three-dimensional cell culture bracket, which can truly simulate the change of temperature and pressure in an animal body when cells are cultured in a three-dimensional environment, thereby enriching a database researched under dynamic cell culture.
To achieve the above objects, the present invention provides a three-dimensional cell culture scaffold comprising: the temperature-sensitive expansion particles are embedded in the bracket body in a powdery form, and the vibration factors are distributed on the outer surface of the bracket body;
the thick fiber phase is a multilayer thick fiber structure, two adjacent layers of the thick fiber phase are arranged in a staggered mode according to a certain angle, the thin fiber phase is independently combined with one or more surfaces of the thick fiber phase, the thick fiber phase forms a hole structure of the three-dimensional cell culture support, the thin fiber phase is uniformly distributed or intensively distributed in the hole structure, and the temperature-sensitive expansion particles have high sensitivity to temperature and can expand and pull the structure of the support body.
The temperature-sensitive swelling particles are copolymer hydrogel.
The vibration factor of the outer surface of the bracket body is a photosensitive nano material, and the photosensitive nano material can vibrate in a light control mode.
The three-dimensional cell culture scaffold composed of the coarse fiber phase is composed of struts and/or fibers of constant or different diameters.
The porosity of the three-dimensional cell culture scaffold is varied by varying the number and size of struts and/or fibers in the scaffold body.
The pore structure of the three-dimensional cell culture scaffold is altered by changing the three-dimensional positioning pattern of struts and/or fibers.
The coarse fiber phase cross-section comprises a circle, a triangle, a rectangle, or any combination thereof.
The three-dimensional cell culture bracket is matched with the sizes of a cell culture flat plate hole, a culture chamber, a culture bottle and/or a bioreactor.
Compared with the prior art, the utility model has the beneficial effects that:
(1) a three-dimensional cell culture support can promote cell adsorption by adding fine fibers with diameters smaller than cell diameters, such as fibers with nanometer diameters, can simulate the change of the structure of the support body in a micro range by embedding temperature-sensitive expansion particles in the support body formed by two materials with different diameters of a coarse fiber phase and a fine fiber phase and controlling the culture temperature in a small range during cell culture, can simulate the change of the growth environment of cells in a living body by applying mechanical stimulation to the cells in the change process, and can simulate the mechanical action of the outside on the cells by arranging vibration factors on the outer surface of the support body to research the influence of fixed stress or strain on cell culture, thereby enriching a database researched under the dynamic cell culture.
(2) The nanofibers have a much smaller diameter than the cells, and are similar in structure to collagen and elastic fibers in the body of a mammal, so that the cells can be easily attached to the nanofibers. The nanofibers can also be effective in promoting differentiation of stem cells thereon. Therefore, the addition of the fibril phase to the three-dimensional cell culture scaffold can improve the efficiency of cell seeding and can promote and regulate the growth and differentiation of cells growing thereon, particularly stem cells.
(3) The copolymer hydrogel is temperature-sensitive hydrogel, can respond to the temperature change, is transparent and biocompatible, can avoid pollution and other influences on cell culture, can generate tensile and compressive mechanical stimulation on cells by using structural stress generated by structural change, and is convenient for researching the cell growth under the stress.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional cell culture scaffold.
In the figure: 1. a coarse fiber phase; 2. a fine fiber phase; 3. temperature sensitive expanding particles.
Detailed Description
The present invention is described in detail with reference to the embodiments shown in the drawings, but it should be understood that these embodiments are not intended to limit the present invention, and those skilled in the art should understand that functional, methodological, or structural equivalents or substitutions made by these embodiments are within the scope of the present invention.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
The utility model provides a three-dimensional cell culture scaffold.
Reference is now made to FIG. 1, which shows a three-dimensional cell culture scaffold according to an embodiment of the present invention.
In this embodiment, a three-dimensional cell culture scaffold comprises: the bracket body is composed of two materials with different diameters of a coarse fiber phase 1 and a fine fiber phase 2, wherein the diameter of the coarse fiber phase 1 is 50 mu m-1mm, temperature-sensitive expansion particles 3 are embedded in the bracket body in a powder form, and vibration factors are distributed on the outer surface of the bracket body; the coarse fiber phase 1 is of a multilayer coarse fiber structure, two adjacent layers of coarse fiber phases 1 are arranged in a staggered mode according to a certain angle, the fine fiber phase 2 is independently combined on one side or multiple sides of the coarse fiber phase 1, the coarse fiber phase 1 forms a hole structure of the three-dimensional cell culture support, the average pore diameter is 50 micrometers-2 mm, the fine fiber phase 2 is uniformly distributed or intensively distributed in the hole structure, and the temperature-sensitive expansion particles 3 have high sensitivity to temperature and can expand and drag the structure of the support body. The temperature-sensitive swelling particles 3 are copolymer hydrogel. The vibration factor of the outer surface of the bracket body is made of photosensitive nano materials, and the photosensitive nano materials can vibrate in a light control mode. The three-dimensional cell culture scaffold consisting of the coarse fiber phase 1 consists of struts and/or fibers of constant or different diameters. The porosity of the three-dimensional cell culture scaffold is varied by varying the number and size of struts and/or fibers in the scaffold body. The pore structure of the three-dimensional cell culture scaffold is altered by changing the three-dimensional positioning pattern of struts and/or fibers. The cross-section of the coarse fiber phase 1 comprises a circle, a triangle, a rectangle, or any combination thereof. The three-dimensional cell culture bracket is matched with the sizes of a cell culture flat plate hole, a culture chamber, a culture bottle and/or a bioreactor.
Specifically, in this embodiment, a three-dimensional cell culture scaffold can promote cell adsorption by adding a fine fiber with a diameter much smaller than that of a cell, such as a fiber with a nanometer diameter, by embedding temperature-sensitive expanded particles 3 in a scaffold body composed of two materials with different diameters, namely a coarse fiber phase 1 and a fine fiber phase 2, and by controlling the culture temperature within a small range during cell culture, the micro-amplitude change of the structure of the scaffold body is realized, mechanical stimulation to the cell is applied by using a change process, the change of the growth environment of the cell in a living body is simulated, and by arranging a vibration factor on the outer surface of the scaffold body, the mechanical action of the outside on the cell is simulated, the influence on cell culture under a fixed stress or strain condition is studied, and thus a database studied under dynamic cell culture is enriched. The nanofibers have a much smaller diameter than the cells, and are similar in structure to collagen and elastic fibers in the body of a mammal, so that the cells can be easily attached to the nanofibers. The nanofibers can also be effective in promoting differentiation of stem cells thereon. Therefore, the addition of the fibril phase 2 to the three-dimensional cell culture scaffold can improve the efficiency of cell seeding and can promote and regulate the growth and differentiation of cells, particularly stem cells, growing thereon. The copolymer hydrogel is temperature-sensitive hydrogel, can respond to the temperature change, is transparent and biocompatible, can avoid pollution and other influences on cell culture, can generate tensile and compressive mechanical stimulation on cells by using structural stress generated by structural change, and is convenient for researching the cell growth under the stress.
The coarse fiber phase 1 and the fine fiber phase 2 are both polymers, inorganic non-metallic materials, metallic materials and composites of the above materials. The polymeric material is preferably made from polymeric materials of polystyrene, poly-racemic lactic acid, copolymers of polylactic acid and glycolide, polycarbonate, polyamide and polyvinyl chloride.
The above-listed detailed description is only a specific description of a possible embodiment of the present invention, and they are not intended to limit the scope of the present invention, and equivalent embodiments or modifications made without departing from the technical spirit of the present invention should be included in the scope of the present invention.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. A three-dimensional cell culture scaffold, comprising: the temperature-sensitive expansion particles are embedded in the bracket body in a powdery form, and the vibration factors are distributed on the outer surface of the bracket body;
the thick fiber phase is a multilayer thick fiber structure, two adjacent layers of the thick fiber phase are arranged in a staggered mode according to a certain angle, the thin fiber phase is independently combined with one or more surfaces of the thick fiber phase, the thick fiber phase forms a hole structure of the three-dimensional cell culture support, the thin fiber phase is uniformly distributed or intensively distributed in the hole structure, and the temperature-sensitive expansion particles have high sensitivity to temperature and can expand and pull the structure of the support body.
2. A three-dimensional cell culture scaffold according to claim 1, wherein: the temperature-sensitive swelling particles are copolymer hydrogel.
3. A three-dimensional cell culture scaffold according to claim 1, wherein: the vibration factor of the outer surface of the bracket body is a photosensitive nano material, and the photosensitive nano material can vibrate in a light control mode.
4. A three-dimensional cell culture scaffold according to claim 1, wherein: the three-dimensional cell culture scaffold composed of the coarse fiber phase is composed of struts and/or fibers of constant or different diameters.
5. The three-dimensional cell culture scaffold according to claim 4, wherein: the porosity of the three-dimensional cell culture scaffold is varied by varying the number and size of struts and/or fibers in the scaffold body.
6. A three-dimensional cell culture scaffold according to claim 1, wherein: the pore structure of the three-dimensional cell culture scaffold is altered by changing the three-dimensional positioning pattern of struts and/or fibers.
7. A three-dimensional cell culture scaffold according to claim 1, wherein: the coarse fiber phase cross-section comprises a circle, a triangle, a rectangle, or any combination thereof.
8. A three-dimensional cell culture scaffold according to claim 1, wherein: the three-dimensional cell culture bracket is matched with the sizes of a cell culture flat plate hole, a culture chamber, a culture bottle and/or a bioreactor.
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| CN202122985205.8U CN216614697U (en) | 2021-11-30 | 2021-11-30 | Three-dimensional cell culture support |
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| CN202122985205.8U CN216614697U (en) | 2021-11-30 | 2021-11-30 | Three-dimensional cell culture support |
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| CN216614697U true CN216614697U (en) | 2022-05-27 |
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