CN216155850U - Fluorescence collecting lens and real-time fluorescence quantitative PCR instrument - Google Patents
Fluorescence collecting lens and real-time fluorescence quantitative PCR instrument Download PDFInfo
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- CN216155850U CN216155850U CN202121846334.2U CN202121846334U CN216155850U CN 216155850 U CN216155850 U CN 216155850U CN 202121846334 U CN202121846334 U CN 202121846334U CN 216155850 U CN216155850 U CN 216155850U
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Abstract
The utility model relates to a fluorescence collecting lens and a real-time fluorescence quantitative PCR instrument using the same. The fluorescence collecting lens comprises a first lens, a second lens, a third lens, a fourth lens, a first optical filter, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object side to an image side; the focal power of the first lens, the fourth lens, the fifth lens and the eighth lens is a negative value, and the focal power of the second lens, the third lens, the sixth lens and the seventh lens is a positive value. The fluorescence collecting lens is applied to a real-time fluorescence quantitative PCR instrument through the combination of 8 lenses, realizes larger numerical aperture of an object space, larger range of an object plane, telecentric object space and favorable for fluorescence collection, and only comprises eight lenses, so that the cost is lower.
Description
Technical Field
The utility model relates to the technical field of molecular diagnosis and detection instruments, in particular to a fluorescence collecting lens and a real-time fluorescence quantitative PCR instrument using the same.
Background
At present, in the technical field of laboratory or medical detection, a method of adding a fluorophore into a Reaction system, detecting the progress of the whole Reaction in real time by using fluorescence signal accumulation, determining the qualitative characteristics of the Reaction system by the intensity of a fluorescence signal, or quantitatively analyzing an unknown sample to be detected according to a standard curve has been widely used, wherein the most typical representative method is real-time fluorescence quantitative PCR (Polymerase Chain Reaction). The real-time fluorescence quantitative PCR instrument has the working principle that excitation light is utilized to irradiate a sample to excite the sample to generate fluorescence, and fluorescence is guided into a photoelectric detector by utilizing a fluorescence collecting lens to obtain biological information. However, some fluorescence collecting lenses and corresponding PCR instruments in the prior art have the defects of small numerical aperture of the object side, small range of the object side, small signal-to-noise ratio and high cost, thereby affecting the fluorescence collecting effect.
SUMMERY OF THE UTILITY MODEL
Aiming at the problems of small numerical aperture of an object side, small range of an object surface, small signal-to-noise ratio, high cost and the like of a fluorescence collecting lens and a corresponding PCR instrument in the prior art, the utility model provides the fluorescence collecting lens for overcoming the defects and the real-time fluorescence quantitative PCR instrument using the fluorescence collecting lens.
A fluorescence collecting lens comprises a first lens, a second lens, a third lens, a fourth lens, a first optical filter, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object side to an image side;
wherein the focal power of the first lens, the fourth lens, the fifth lens and the eighth lens is a negative value, and the focal power of the second lens, the third lens, the sixth lens and the seventh lens is a positive value.
In one embodiment, the first lens and the second lens are mutually glued to form a first lens combination, and the focal power of the first lens combination is a positive value;
the fifth lens and the sixth lens are mutually glued to form a second lens combination, and the focal power of the second lens combination is a positive value.
In one embodiment, the object-side surface of the first lens element is concave, and the image-side surface of the first lens element is convex;
the object side surface of the second lens is a concave surface, and the image side surface of the second lens is a convex surface;
the object side surface of the third lens is a convex surface, and the image side surface of the third lens is a convex surface;
the object side surface of the fourth lens is a concave surface, and the image side surface of the fourth lens is a convex surface;
the object side surface of the fifth lens is a convex surface, and the image side surface of the fifth lens is a concave surface;
the object side surface of the sixth lens is a convex surface, and the image side surface of the sixth lens is a convex surface;
the object side surface of the seventh lens is a convex surface, and the image side surface of the seventh lens is a concave surface;
the object side surface of the eighth lens is a concave surface, and the image side surface of the eighth lens is a concave surface.
In one embodiment, the fluorescence collection lens satisfies: 1.6 < f12/f＜2.4；1.5＜f3/f＜2.0；2.5＜|f4/f|＜3.0；2.0＜f66/f＜3.0；0.5＜f7/f＜1.1；0.8＜|f8/f|＜1.4；
Wherein f represents the focal length of the fluorescence collecting lens, f12Denotes the focal length of the first lens combination, f3Denotes the focal length of the third lens, f4Denotes the focal length of the fourth lens, f56Denotes the focal length of the second lens combination, f7Denotes a focal length of the seventh lens, f8Denotes a focal length of the eighth lens.
In one embodiment, the refractive index of the first lens is greater than or equal to 1.84 and less than or equal to 2.01, and the abbe number of the first lens is greater than or equal to 17 and less than or equal to 25;
the refractive index of the second lens is greater than or equal to 1.84 and less than or equal to 2.01, and the Abbe number of the second lens is greater than or equal to 17 and less than or equal to 45;
the refractive index of the third lens is greater than or equal to 1.63 and less than or equal to 1.80, and the Abbe number of the third lens is greater than or equal to 45 and less than or equal to 65;
the refractive index of the fourth lens is greater than or equal to 1.75 and less than or equal to 1.95, and the Abbe number of the fourth lens is greater than or equal to 17 and less than or equal to 30;
the refractive index of the fifth lens is greater than or equal to 1.84 and less than or equal to 2.01, and the Abbe number of the fifth lens is greater than or equal to 17 and less than or equal to 30;
the refractive index of the sixth lens is greater than or equal to 1.63 and less than or equal to 1.80, and the Abbe number of the sixth lens is greater than or equal to 45 and less than or equal to 65;
the refractive index of the seventh lens is greater than or equal to 1.90 and less than or equal to 2.01, and the Abbe number of the seventh lens is greater than or equal to 17 and less than or equal to 40;
the refractive index of the material of the eighth lens is greater than or equal to 1.75 and less than or equal to 1.95, and the Abbe number of the eighth lens is greater than or equal to 17 and less than or equal to 30.
In one embodiment, the fluorescence collecting lens further includes a filter wheel, the filter wheel is provided with a plurality of first filters, and each first filter can rotate to a position between the fourth lens and the fifth lens along with the filter wheel.
In one embodiment, a first spacer is arranged between the second lens and the third lens, and a second spacer is arranged between the sixth lens and the seventh lens;
the first and second spacers have a coefficient of thermal expansion greater than or equal to 60 x 106/℃。
A real-time fluorescence quantitative PCR instrument comprises a light source component, a first light guide optical fiber bundle, a fluorescence collecting lens and a photoelectric detector, wherein the fluorescence collecting lens is arranged in any embodiment;
the light source assembly is used for irradiating each sample, so that each sample is excited to emit fluorescence respectively; one end of the first light guide optical fiber bundle is arranged corresponding to the plurality of samples and is used for respectively receiving the fluorescence emitted by each sample, and the other end of the first light guide optical fiber bundle is arranged at the object side of the fluorescence collecting lens; the photoelectric detectors are arranged on the image side of the fluorescence collecting lens.
In one embodiment, the photodetector comprises a SiPM array or MPPC array.
In one embodiment, the light source assembly includes an excitation light source, a mirror group disposed on a light emitting side of the excitation light source, and a second light guiding fiber bundle, one end of the second light guiding fiber bundle is disposed on a light emitting side of the mirror group, and the other end of the second light guiding fiber bundle is disposed corresponding to the plurality of samples.
In one embodiment, the lens group includes a TIR lens, a double fly-eye lens, a fresnel lens, a second optical filter, a plano-convex lens, and a glass light cone, which are sequentially disposed from a light incident side to a light emergent side of the lens group.
According to the fluorescence collection lens and the real-time fluorescence quantitative PCR instrument, a plurality of samples are respectively excited to generate fluorescence, and the fluorescence is transmitted by the first light guide fiber bundle and then sequentially passes through the first lens, the second lens, the third lens, the fourth lens, the first optical filter, the fifth lens, the sixth lens, the seventh lens and the eighth lens to reach the image side of the fluorescence collection lens. The fluorescence at the object side is gradually folded and collimated through the first lens, the second lens, the third lens and the fourth lens, and approximately parallel light is formed; then the light beams pass through the first optical filter and then gradually converge when passing through the fifth lens, the sixth lens, the seventh lens and the eighth lens, and finally fall on the photoelectric detector. The fluorescence collecting lens realizes larger numerical aperture of an object space, larger range of an object plane and telecentric object space through the combination of 8 lenses, is favorable for fluorescence collection, and only comprises eight lenses, so the cost is lower.
Drawings
FIG. 1 is a schematic diagram of an optical path of a fluorescence collecting lens according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of the fluorescence collection lens shown in FIG. 1;
FIG. 3 is a dot-sequence diagram of the fluorescence collection lens shown in FIG. 1;
FIG. 4 is a graph of the MTF of the fluorescence collection lens of FIG. 1;
FIG. 5 is a field curvature/distortion diagram of the fluorescence collection lens shown in FIG. 1;
FIG. 6 is a schematic view of a structure of the fluorescence collecting lens shown in FIG. 1 engaged with a first light guiding fiber bundle;
FIG. 7 is an end view of the output end of the first light directing optical fiber bundle shown in FIG. 6;
fig. 8 is a schematic structural diagram of a light source module according to an embodiment of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Referring to fig. 1 and fig. 2, an embodiment of the utility model provides a fluorescence collecting lens 10, which includes a first lens 11, a second lens 12, a third lens 13, a fourth lens 14, a first filter 19, a fifth lens 15, a sixth lens 16, a seventh lens 17, and an eighth lens 18, which are sequentially disposed from an object side a to an image side b. The focal power of the first lens 11, the fourth lens 14, the fifth lens 15 and the eighth lens 18 is a negative value, and the focal power of the second lens 12, the third lens 13, the sixth lens 16 and the seventh lens 17 is a positive value.
In the fluorescence collecting lens 10, the plurality of samples are excited to generate fluorescence, and the fluorescence passes through the first lens 11, the second lens 12, the third lens 13, the fourth lens 14, the first optical filter 19, the fifth lens 15, the sixth lens 16, the seventh lens 17, and the eighth lens 18 in sequence after being transmitted by the first light guide fiber bundle, and then reaches the image side of the fluorescence collecting lens 10. The fluorescence at the object side a is gradually converged and collimated by the first lens 11, the second lens 12, the third lens 13 and the fourth lens 14 to form approximately parallel light, and then gradually converged by the fifth lens 15, the sixth lens 16, the seventh lens 17 and the eighth lens 18, and finally falls on the photodetector. The fluorescence collecting lens 10 of the utility model realizes larger numerical aperture of an object space, larger range of an object plane and telecentric object space by combining 8 lenses, is beneficial to fluorescence collection, and only comprises eight lenses, thereby having lower cost.
In the embodiment of the present invention, the first lens 11 and the second lens 12 are cemented with each other to form the first lens combination, and the focal power of the first lens combination is a positive value. The fifth lens 15 and the sixth lens 16 are cemented to each other to form a second lens combination having a positive power. Thus, the fluorescent light collecting effect is ensured, and the structure of the fluorescent light collecting lens 10 is more compact.
In an embodiment, the object-side surface of the first lens element 11 is concave, and the image-side surface thereof is convex. The object-side surface of the second lens element 12 is concave, and the image-side surface thereof is convex. The object-side surface of the third lens element 13 is convex, and the image-side surface thereof is convex. The fourth lens element 14 has a concave object-side surface and a convex image-side surface. The fifth lens element 15 has a convex object-side surface and a concave image-side surface. The sixth lens element 16 has a convex object-side surface and a convex image-side surface. The seventh lens element 17 has a convex object-side surface and a concave image-side surface. The eighth lens element 18 has a concave object-side surface and a concave image-side surface.
In particular embodiments, the fluorescence collecting lens 10 satisfies: 1.6 < f12/f＜2.4；1.5＜f3/f＜2.0；2.5＜|f4/f|＜3.0；2.0＜f66/f＜3.0；0.5＜f7/f＜1.1；0.8＜|f8The/| is less than 1.4. Wherein f denotes a focal length of the fluorescence collecting lens 10, f12Denotes the focal length of the first lens combination, f3Denotes the focal length, f, of the third lens 134Denotes the focal length, f, of the fourth lens 1456Denotes the focal length of the second lens combination, f7Denotes a focal length, f, of the seventh lens 178Indicating the focal length of the eighth lens 18. Thus, by controlling the focal length of each lens, the object-side numerical aperture of the fluorescence collecting lens 10 is further made larger (up to 0.56), the object-side range is larger (up to phi 7.4mm), and the object-side is telecentric, which is beneficial to collecting fluorescence.
In addition, | f4F represents f4Absolute value of/f, | f8F represents f8Absolute value of/f.
Further, the refractive index of the first lens 11 is greater than or equal to 1.84 and less than or equal to 2.01; the abbe number of the first lens 11 is greater than or equal to 17 and less than or equal to 25; the refractive index of the second lens 12 is greater than or equal to 1.84 and less than or equal to 2.01; the abbe number of the second lens 12 is greater than or equal to 17 and less than or equal to 45; the refractive index of the third lens 13 is greater than or equal to 1.63 and less than or equal to 1.80; the abbe number of the third lens 13 is greater than or equal to 45 and less than or equal to 65; the refractive index of the fourth lens 14 is greater than or equal to 1.75 and less than or equal to 1.95; the abbe number of the fourth lens 14 is greater than or equal to 17 and less than or equal to 30; the refractive index of the fifth lens 15 is greater than or equal to 1.84 and less than or equal to 2.01; the abbe number of the fifth lens 15 is greater than or equal to 17 and less than or equal to 30; the refractive index of the sixth lens 16 is greater than or equal to 1.63 and less than or equal to 1.80; the abbe number of the sixth lens 16 is 45 or more and 65 or less; the refractive index of the seventh lens 17 is greater than or equal to 1.90 and less than or equal to 2.01; the abbe number of the seventh lens 17 is greater than or equal to 17 and less than or equal to 40; the refractive index of the material of the eighth lens 18 is greater than or equal to 1.75 and less than or equal to 1.95; the abbe number of the eighth lens 18 is greater than or equal to 17 and less than or equal to 30.
In the embodiment of the utility model, the first filter 19 included in the fluorescence collecting lens 10 is disposed between the fourth lens 14 and the fifth lens 15. In this way, the light rays form approximately parallel light after passing through the first lens 11 to the fourth lens 14, and the incident angle of the light rays entering the first filter 19 is not large.
In the embodiment, a larger gap is formed between each of the fourth lens 14 and the fifth lens 15 and the first filter 19, so that the first filters 19 of different types can be replaced conveniently, thereby obtaining fluorescence of different wavelength bands and improving the compatibility of the fluorescence collecting lens 10. Optionally, a gap between each of the fourth lens 14 and the fifth lens 15 and the first filter 19 is greater than or equal to 3.0 mm.
It should be noted that the first filter 19 can be switched by using the filter wheel 191, that is, the first filters 19 of different types are mounted on the filter wheel 191, and each first filter 19 can be located between the fourth lens 14 and the fifth lens 15 by the rotation of the filter wheel 191, so as to switch the first filters 19.
In the embodiment of the present invention, the first spacer 12 is disposed between the second lens 12 and the third lens 131, a second spacer 161 is provided between the sixth lens 16 and the seventh lens 17. And the thermal expansion coefficient of the first space ring 121 and the second space ring 161 is greater than or equal to 60 × 106V. C. In this manner, the thermal expansion coefficients of the first and second spacers 121 and 161 are designed to be large, i.e., greater than or equal to 60 × 106The temperature per degree centigrade is favorable for utilizing the larger thermal expansion of the first space ring 121 and the second space ring 161 to offset the influence of the thermal expansion of other components, thereby eliminating the influence of the environmental temperature on the performance of the fluorescence collecting lens 10 and improving the weather resistance of the fluorescence collecting lens 10. The fluorescence collecting lens 10 of the present invention can normally operate in a temperature range of-40 ℃ to +70 ℃.
Further, the thermal expansion coefficient of the space ring between the lens barrel and other lenses is smaller than that of the first space ring 121 and the second space ring 161. Thus, the thermal expansion of the entire fluorescence collecting lens 10 is at a low level, and the weather resistance of the fluorescence collecting lens 10 is further improved. Optionally, the coefficient of thermal expansion of the spacer between the lens barrel and the other lens is less than or equal to 23.2 × 106/℃。
In one embodiment, the basic parameters of each lens from the object side to the image side of the fluorescence collecting lens 10 are as shown in table 1, and the unit: and (4) millimeter.
TABLE 1 detailed parameters of the respective mirror surfaces of the fluorescence collecting lens 10
The focal length EFL of the fluorescence collecting lens 10 adopting the basic parameters in table 1 is 17.20mm @620nm, the diameter of the object plane is Φ 7.4mm, the f-number FNO is 0.82, the numerical aperture NA of the object side is 0.56, the object space is telecentric, the conjugate distance of the lens object image is 90.00mm, and when the MPPC array is matched, the incident angle CRA of the image plane chief ray is less than or equal to 7.75 °.
Fig. 3 is a dot-column diagram of the fluorescence collecting lens 10 in this embodiment, in which the wavelengths are 500nm, 620nm, and 740nm, and the weight ratio is 1:1: 1. As can be seen from FIG. 3, the scattered spots in each field are relatively concentrated, the root mean square radius is less than 12 μm, and the distribution is relatively uniform.
Fig. 4 is a Modulation Transfer Function (MTF) graph in visible light. The MTF graph represents the comprehensive resolution level of an optical system, and as can be seen from FIG. 4, the MTF values of the central field and the edge field at 30lp/mm are both greater than or equal to 0.4.
Fig. 5 is a graph of field curvature/distortion under visible light. The distortion curve graph represents the magnitude of distortion in% for different angles of view. As can be seen from FIG. 5, the optical distortion is positive and has a value of 2.2% or less.
In another embodiment, the basic parameters of each lens from the object side to the image side of the fluorescence collecting lens 10 are as shown in table 2, with the unit: and (4) millimeter.
TABLE 2 detailed parameters of the respective mirror surfaces of the fluorescence collecting lens 10
The focal length value of the fluorescence collecting lens 10 adopting the basic parameters in table 2 is 18.66mm @620nm, the diameter of the object plane is phi 7.4mm, the aperture value is FNO is 0.81, the numerical aperture NA of the object space is 0.56, the object space is telecentric, the conjugate distance of the lens object image is 94.93mm, and when the MPPC array is matched, the incident angle CRA of the image plane chief ray is less than or equal to 6.97 °.
In still another embodiment, basic parameters of each lens from the object side to the image side of the fluorescence collecting lens 10 are as shown in table 3, in units of: and (4) millimeter.
TABLE 3 detailed parameters of the respective mirror surfaces of the fluorescence collecting lens 10
The focal length value of the fluorescence collecting lens 10 adopting the basic parameters in table 3 is EFL 16.85mm @620nm, the diameter of the object plane is Φ 7.4mm, the aperture value is FNO 0.81, the numerical aperture NA of the object space is 0.56, the object space is telecentric, the conjugate distance of the lens object image is 95.00mm, and when the MPPC array is matched, the incident angle CRA of the image plane chief ray is less than or equal to 8.36 °.
Referring to fig. 6 to 8, based on the fluorescence collecting lens 10, the present invention further provides a real-time fluorescence quantitative PCR instrument, which includes a light source assembly 30, a first light guiding fiber bundle 20, the fluorescence collecting lens 10 as described in any of the embodiments above, and a photodetector 40.
The light source assembly 30 is disposed at one side of the plurality of samples, and is used to irradiate each of the samples 100, respectively, such that each of the samples 100 is excited to generate fluorescence, respectively. One end of the first light guiding fiber bundle 20 is arranged corresponding to the plurality of samples and is used for respectively receiving the fluorescence emitted by exciting each sample 100; the other end of the first light guiding fiber bundle 20 is disposed at the object side of the fluorescence collecting lens 10, so that the fluorescence directly enters the fluorescence collecting lens 10 after being conducted by the first light guiding fiber bundle 20. The photodetector 40 is arranged on the image side of the fluorescence collecting lens 10.
Specifically, in the embodiment, the first light guiding fiber bundle 20 includes a plurality of light guiding fibers 21, an input end 201 of each light guiding fiber 21 is arranged corresponding to one sample 100, that is, fluorescence generated after each sample 100 is excited is received by a corresponding light guiding fiber 21, and the light guiding fibers 21 are arranged in an array at an output end 202 to form an object plane.
Further, the first light guiding fiber bundle 20 includes 16 light guiding fibers 21, the 16 light guiding fibers 21 are used to receive the fluorescence generated by the 16 samples 100, and the 16 light guiding fibers 21 are arranged in a 4 × 4 array at the output end 201 to form an object plane. The effective diameter of each light guide fiber 21 is 1.00mm, and the diameter of the metal sleeve binding each light guide fiber 21 is 1.30mm, so the length and width of the object plane is 5.20mm × 5.20 mm.
In particular embodiments, the photodetectors 40 may employ a SiPM array or an MPPC array. Thus, compared with a CCD (Charge Coupled Device) camera in the prior art, the SiPM array or MPPC array has a larger dynamic range and higher sensitivity. More specifically, the fluorescence collecting lens 10 corresponds the output ends of the light guide fibers arranged in an array to an SiPM array (silicon photomultiplier array) or an MPPC array (multi-pixel photon counter array) one by one.
Referring to fig. 8, in the embodiment, the light source assembly 30 includes an excitation light source 31, a mirror group 32 and a second light guiding fiber bundle 33. The lens group 32 is disposed on the light emitting side of the excitation light source 31, and is configured to perform processing such as collimation, light uniformization, convergence, and filtering on light emitted from the excitation light source 31. One end of the second light guiding fiber bundle 33 is disposed at the light exit side of the mirror group 32, so that the light emitted from the mirror group 32 enters the second light guiding fiber bundle 33; the other end of the second light guiding fiber bundle 33 corresponds to each sample 100, so that the light emitted from the second light guiding fiber bundle 33 irradiates each sample 100, thereby exciting each sample 100 to generate fluorescence.
Alternatively, the excitation light source 31 may be a full spectrum COB light source, preferably a COB light source with a color temperature around 5000K, and the spectrum of the COB light source is relatively flat in the visible light band, so that after filtering, the intensity difference of excitation light with different colors is relatively small. Moreover, the COB light source has long service life, usually 4 to 6 ten thousand hours, and is hardly influenced by repeated switching.
More specifically, the lens assembly 32 includes a tir (total internal reflection) lens 321, a double fly-eye lens 322, a fresnel lens 323, a second filter 324, a plano-convex lens 325 and a glass light cone 326 sequentially disposed from the light incident side to the light emergent side of the lens assembly 32. The TIR lens 321 is used for collimating the light, and the fly-eye lens 322 is used for dodging the collimated light. The fresnel lens 323 is used to collect the homogenized light for the first time. The second filter 324 filters the light after the first convergence. The plano-convex lens 325 and the glass cone 326 are used to collect the filtered light for the second time.
In an embodiment, second light guiding fiber bundle 33 has a large end 331 and a plurality of branch ends 332 (e.g. 16 branch ends 332), where large end 331 is disposed on the light output side of optical lens group 32, so that the light emitted from the light output side of optical lens group 32 directly enters large end 331 and exits from each branch end 332. Each branch end 332 is disposed corresponding to one sample 100, so that the light emitted from each branch end 332 irradiates the corresponding sample 100 to excite each sample.
The fluorescence collecting lens 10 and the real-time fluorescence quantitative PCR instrument using the fluorescence collecting lens 10 have the following advantages:
1) the size of the object surface of the fluorescence collecting lens 10 can reach phi 7.4mm, the F number can reach 0.82, the numerical aperture of the object side can reach 0.56, and the object space is telecentric, so that the fluorescence can be collected.
2) The fluorescence collecting lens 10 comprises 8 lenses, and is small in quantity and moderate in cost. The surface of the lens is plated with a plurality of layers of broadband antireflection films, and the spectral reflectivity of the visible light wave band is even lower than 0.1 percent. When a plurality of samples are detected simultaneously, the fluorescence collecting lens 10 can fully utilize fluorescence, reduce crosstalk between adjacent optical fibers and improve the signal-to-noise ratio of the real-time fluorescence quantitative PCR instrument.
3) And an SiPM array or an MPPC array is adopted, so that the dynamic range is large and the sensitivity is high.
4) The excitation light source 31 is a full spectrum COB light source, preferably with a color temperature around 5000K, and its spectrum is relatively flat in the visible light band, so that after filtering, the intensity difference of the excitation light of different colors is small. The excitation light source 31 preferably passes through a COB light source of the LM-80 standard, has a long operating life, typically 4 to 6 ten thousand hours, and is hardly affected by repeated switching.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (11)
1. A fluorescence collecting lens is characterized by comprising a first lens, a second lens, a third lens, a fourth lens, a first optical filter, a fifth lens, a sixth lens, a seventh lens and an eighth lens which are sequentially arranged from an object side to an image side;
wherein the focal power of the first lens, the fourth lens, the fifth lens and the eighth lens is a negative value, and the focal power of the second lens, the third lens, the sixth lens and the seventh lens is a positive value.
2. The fluorescence collection lens of claim 1, wherein the first lens and the second lens are cemented to each other to form a first lens combination, the first lens combination having a positive optical power;
the fifth lens and the sixth lens are mutually glued to form a second lens combination, and the focal power of the second lens combination is a positive value.
3. The fluorescence collection lens of claim 2, wherein the first lens element has a concave object-side surface and a convex image-side surface;
the object side surface of the second lens is a concave surface, and the image side surface of the second lens is a convex surface;
the object side surface of the third lens is a convex surface, and the image side surface of the third lens is a convex surface;
the object side surface of the fourth lens is a concave surface, and the image side surface of the fourth lens is a convex surface;
the object side surface of the fifth lens is a convex surface, and the image side surface of the fifth lens is a concave surface;
the object side surface of the sixth lens is a convex surface, and the image side surface of the sixth lens is a convex surface;
the object side surface of the seventh lens is a convex surface, and the image side surface of the seventh lens is a concave surface;
the object side surface of the eighth lens is a concave surface, and the image side surface of the eighth lens is a concave surface.
4. The fluorescence collection lens of claim 3, wherein the fluorescence collection lens satisfies: 1.6 < f12/f＜2.4；1.5＜f3/f＜2.0；2.5＜|f4/f|＜3.0；2.0＜f56/f＜3.0；0.5＜f7/f＜1.1；0.8＜|f8/f|＜1.4；
Wherein f represents the focal length of the fluorescence collecting lens, f12Denotes the focal length of the first lens combination, f3Denotes the focal length of the third lens, f4Denotes the focal length of the fourth lens, f56Denotes the focal length of the second lens combination, f7Denotes a focal length of the seventh lens, f8Denotes a focal length of the eighth lens.
5. The fluorescence collecting lens according to claim 4, wherein the refractive index of the first lens is 1.84 or more and 2.01 or less, and the Abbe number of the first lens is 17 or more and 25 or less;
the refractive index of the second lens is greater than or equal to 1.84 and less than or equal to 2.01, and the Abbe number of the second lens is greater than or equal to 17 and less than or equal to 45;
the refractive index of the third lens is greater than or equal to 1.63 and less than or equal to 1.80, and the Abbe number of the third lens is greater than or equal to 45 and less than or equal to 65;
the refractive index of the fourth lens is greater than or equal to 1.75 and less than or equal to 1.95, and the Abbe number of the fourth lens is greater than or equal to 17 and less than or equal to 30;
the refractive index of the fifth lens is greater than or equal to 1.84 and less than or equal to 2.01, and the Abbe number of the fifth lens is greater than or equal to 17 and less than or equal to 30;
the refractive index of the sixth lens is greater than or equal to 1.63 and less than or equal to 1.80, and the Abbe number of the sixth lens is greater than or equal to 45 and less than or equal to 65;
the refractive index of the seventh lens is greater than or equal to 1.90 and less than or equal to 2.01, and the Abbe number of the seventh lens is greater than or equal to 17 and less than or equal to 40;
the refractive index of the material of the eighth lens is greater than or equal to 1.75 and less than or equal to 1.95, and the Abbe number of the eighth lens is greater than or equal to 17 and less than or equal to 30.
6. The fluorescence collecting lens according to any one of claims 1 to 5, further comprising a filter wheel, wherein a plurality of the first filters are mounted on the filter wheel, and each of the first filters can rotate along with the filter wheel to a position between the fourth lens and the fifth lens.
7. The fluorescence collection lens of any of claims 1 to 5, wherein a first spacer is disposed between the second lens and the third lens, and a second spacer is disposed between the sixth lens and the seventh lens;
the first and second spacers have a coefficient of thermal expansion greater than or equal to 60 x 106/℃。
8. A real-time fluorescence quantitative PCR instrument, comprising a light source assembly, a first light guiding fiber bundle, the fluorescence collecting lens according to any one of claims 1 to 7, and a photodetector;
the light source assembly is used for irradiating a plurality of samples, so that each sample is respectively excited to emit fluorescence; one end of the first light guide optical fiber bundle is arranged corresponding to the plurality of samples and is used for respectively receiving the fluorescence emitted by each sample, and the other end of the first light guide optical fiber bundle is arranged at the object side of the fluorescence collecting lens; the photoelectric detectors are arranged on the image side of the fluorescence collecting lens.
9. The real-time fluorescent quantitative PCR instrument of claim 8, wherein the photodetector comprises a SiPM array or an MPPC array.
10. The real-time fluorescence quantitative PCR instrument of claim 8, wherein the light source assembly comprises an excitation light source, a mirror group and a second light guiding fiber bundle, the mirror group is disposed on a light emitting side of the excitation light source, one end of the second light guiding fiber bundle is disposed on a light emitting side of the mirror group, and the other end of the second light guiding fiber bundle is disposed corresponding to the plurality of samples.
11. The real-time fluorescence quantitative PCR instrument of claim 10, wherein the lens group comprises a TIR lens, a double fly-eye lens, a Fresnel lens, a second optical filter, a plano-convex lens and a glass light cone which are sequentially arranged from the light-in side to the light-out side of the lens group.
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| CN202121846334.2U CN216155850U (en) | 2021-08-09 | 2021-08-09 | Fluorescence collecting lens and real-time fluorescence quantitative PCR instrument |
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| CN202121846334.2U CN216155850U (en) | 2021-08-09 | 2021-08-09 | Fluorescence collecting lens and real-time fluorescence quantitative PCR instrument |
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