CN215004989U

CN215004989U - Microfluid chip and platelet function detection device

Info

Publication number: CN215004989U
Application number: CN202120541348.7U
Authority: CN
Inventors: 徐轶; 吴志刚
Original assignee: Huazhong University of Science and Technology
Current assignee: Huazhong University of Science and Technology
Priority date: 2021-03-16
Filing date: 2021-03-16
Publication date: 2021-12-03
Anticipated expiration: 2031-03-16

Abstract

The utility model discloses a microfluid chip and platelet function detection device belongs to platelet function detection technical field. The micro-fluidic shear force control device comprises a platelet separation and enrichment module, a micro-fluidic shear force control module and a collagen control module, red and white blood cells and platelets are separated by utilizing a micro-fluidic inertia force induction mode, and the platelets are activated under the action of large shear force in a micro-channel by designing the depth-to-width ratio of the micro-channel; the collagen control module enables platelets to generate an adhesion aggregation phenomenon, the fluorescence microscopic component can detect the area and height of platelet aggregation after fluorescent staining, the aggregation rate of the platelets is calculated, and the mapping relation between the time of thrombus formation and the collected image is established to reflect the adhesion aggregation function level of the platelets. The utility model discloses utilize microfluid inertial force separation purification platelet to realize the control to platelet adhesion gathering dynamics through control collagen content, realize clinical diagnosis and anti platelet medicine pharmacodynamic evaluation to thrombotic diseases.

Description

Microfluid chip and platelet function detection device

Technical Field

The utility model belongs to the technical field of the platelet function detects, more specifically relates to a microfluid chip and platelet function detection device.

Background

Thrombotic diseases seriously threaten the life and health of human beings, the incidence rate of the thrombotic diseases is the first of various diseases, and the thrombotic diseases are increasing in recent years, and are one of the key points and hot points of modern medical research. At present, the platelet aggregation function has become one of the clinical basic detection items, and the detection item provides a favorable basis for the diagnosis and treatment of thrombotic diseases. With the development of society and science, accurate medical treatment has gradually become the development trend of medical detection, and Point of care (Point of care) provides a very good prerequisite for accurate medical treatment.

Platelets are important components of blood clotting and hemostatic functions, and activation and adhesive aggregation of platelets play an important role in the process of thrombosis or primary hemostasis. At present, the platelet aggregation function has become one of the basic clinical detection items, and the existing detection instruments and systems mainly include a platelet function analyzer (PFA-100), a Verify Now measuring instrument, a platelet measuring instrument, a Multiplate measuring instrument, and the like. However, these devices have their own advantages and disadvantages, and to date, there is no standard, widely recognized worldwide, pre-operative instant platelet function monitor. Although the Verify Now measuring instrument can directly analyze blood without centrifuging and purifying platelets, the measurement process needs to test various target inducers respectively, which is time-consuming and clinically shows that the prediction result is not accurate. Both the Plateleworks measuring instrument and the Multiplate measuring instrument need to be used when the number of platelets reaches a certain value, namely, the blood needs to be centrifuged, purified and the like to ensure the detection accuracy. The testing systems are tested under the condition of static or irrelevant shearing force in vivo, and have larger difference with the flowing environment where the platelets in vivo are adhered and aggregated. Meanwhile, the exact kinetic mechanism of the influence of antiplatelet drugs on the platelet adhesion aggregation behavior under the in vivo physiologically relevant flow conditions is not clear. PFA-100 is an assay system that is closer to physiological environment than the platelet adhesion aggregation caused by the stimulation of platelet activation inducing substances alone at present. However, this system cannot adjust the data fluctuation and the concentration of the inducing substance contained in the blood passing through the hole, and therefore, it is difficult to ensure the accuracy of measuring the platelet function in the patient with the platelet function abnormality. More importantly, this is very different from the physiological platelet activation environment, and although it is possible to measure significant functional differences such as congenital dysfunction of various receptors, it is difficult to measure and reflect detailed platelet function of the biological condition when platelets are activated in vivo and the number of platelets is reduced, or when the number of platelets is normal but the platelet function is weak.

SUMMERY OF THE UTILITY MODEL

To the above defect of prior art or improve the demand, the utility model provides a microfluid chip and platelet function detection device, its aim at utilizes microfluid inertial force induction mode to realize the separation of platelet, solves among the prior art platelet and leukocyte's complex from this and leads to the inaccurate technical problem of testing result.

To achieve the above object, according to an aspect of the present invention, there is provided a microfluidic chip including: a microfluidic main channel and a microfluidic channel;

the end of the microfluidic main channel is communicated with the microfluidic channel;

the micro-fluid main channel is provided with a blood injection port, a first regulating micro-fluid channel and a second regulating micro-fluid channel; the blood injection port is arranged at the initial end of the microfluid main channel; the first adjusting microfluidic channel and the second adjusting microfluidic channel are oppositely arranged at two sides of the microfluidic flow direction and are respectively used for injecting first adjusting liquid and second adjusting liquid with different flow rates;

the tail end of the microfluid channel is provided with a platelet separation and purification port and a waste liquid collection port;

separating platelets from red blood cells and white blood cells in a blood sample by microfluidic inertial force induction by adjusting the flow rates of the first and second conditioning liquids injected into the first and second conditioning microfluidic channels; and extracting the separated platelets from the platelet separation and purification port, thereby realizing the separation of the platelets in the blood sample.

Preferably, in order to achieve a significant separation effect of platelets from red blood cells and white blood cells, the liquid injected into the first regulating microfluidic channel is a high-viscosity liquid, and the liquid injected into the second regulating microfluidic channel is a low-viscosity liquid.

Preferably, the microfluidic channel is configured as a groove structure for adhesion aggregation of platelets separated from the blood sample.

Preferably, the cross section of the groove structure is in any shape such as concave shape and U shape, the depth of the groove structure of the microfluidic channel is 10-100 μm, and the width of the groove structure of the microfluidic channel is 20-250 μm.

Preferably, the microfluidic chip further comprises a microchannel, an inlet of the microchannel is communicated with the platelet separation and purification port, and an outlet of the microchannel is provided with a platelet waste liquid collection port. Further preferably, the micro-channel and the micro-fluid channel are integrally formed, and seamless connection can be realized.

Preferably, the microchannel is provided with a groove structure, and the groove structure of the microchannel is used for enabling the flow of the platelets in the microchannel to be equivalent to the laminar flow of the incompressible Newtonian fluid in the rectangular microchannel, so that the purified platelets can be self-activated under the action of the shear force.

Preferably, the cross section of the groove structure of the microchannel is in any shape such as concave shape, U shape and the like, and the aspect ratio of the groove structure is 1: 10; further preferably, the width of the microchannel trench structure is 240 μm.

Preferably, the kit further comprises a drug injection port, wherein the drug injection port is communicated with the microchannel and used for injecting drugs into the microchannel so as to realize the detection of the platelet function inhibition under the action of different drugs.

Preferably, the kit further comprises a collagen control module, wherein the collagen control module is arranged at the microchannel and is used for testing the adhesion of collagen to the platelets under different concentrations. Further preferably, the concentration of the collagen is controlled between 100 mug/mL and 250 mug/mL.

According to another aspect of the present invention, there is provided a platelet function detection device, the detection device including a microfluidic chip, further including: the injection device comprises an upper layer substrate, a lower layer substrate, a first injection assembly, a second injection assembly, a third injection assembly and a fourth injection assembly;

the upper substrate is coupled with the lower substrate, the micro-fluid main channel, the micro-fluid channel and the micro-channel are arranged on the upper substrate, and the collagen control module is arranged on the lower substrate;

the first injection assembly is communicated with the blood injection port and is used for injecting a blood sample into the microfluidic main channel;

the second injection assembly is communicated with the second regulating micro-fluid channel and is used for injecting a second regulating liquid into the micro-fluid main channel and controlling the flow rate of the second regulating liquid;

the third injection assembly is communicated with the first regulating micro-fluid channel and is used for injecting a first regulating liquid into the micro-fluid main channel and controlling the flow rate of the first regulating liquid;

the fourth injection assembly is communicated with the drug injection port and is used for injecting drugs for detecting the platelet function inhibition into the drug injection port.

Preferably, the collagen control module comprises a first collagen microchannel and a second collagen microchannel in communication with each other on the lower substrate and a fifth injection assembly in communication with the second collagen microchannel; the first collagen microchannel is positioned above the second collagen microchannel and is communicated with the microchannel; the fifth injection assembly is used for injecting collagen liquid into the second collagen micro-channel and controlling the concentration of the collagen liquid.

Preferably, the kit further comprises a fluorescence microscopic component, wherein the fluorescence microscopic component is arranged above the upper substrate and is focused on the microchannel, and is used for observing the change and the image of the platelet adhesion aggregation morphology.

According to another aspect of the present invention, there is provided a platelet function detection method, comprising the steps of:

injecting a blood sample into the microfluidic main channel, and separating platelets from red blood cells and white blood cells in the blood sample by regulating the flow rate and viscosity of liquid injection in the first regulating microfluidic channel and the second regulating microfluidic channel and utilizing a microfluidic inertia force induction mode;

when the separated platelets flow through the first collagen microchannel from the microchannel, the platelets and collagen interact to realize adhesion and aggregation, and the detection of the platelet function is realized by observing the change of the adhesion and aggregation state of the platelets and images.

Generally, through the utility model discloses above technical scheme who conceives compares with prior art, can gain following beneficial effect:

1. the utility model provides a microfluid chip utilizes the induced mode of microfluid inertial force to make platelet and red blood cell, the separation of leucocyte in the blood sample through adjusting the velocity of flow and the viscidity that liquid injected into in first regulation microfluid passageway and the second regulation microfluid passageway, avoids platelet to detect in the separation of platelet blood sample thoroughly to lead to the testing result inaccurate.

2. The utility model provides a microfluid chip is through setting up the microchannel to the groove structure, makes the flow equivalence of platelet in the microchannel be the laminar flow of incompressible Newtonian fluid in the rectangle microchannel and flows, and the concentration of the inductive material that contains in the blood through control collagen content regulation data fluctuation and through the hole realizes the control to platelet adhesion gathering dynamics.

3. The utility model provides a microfluid chip is through integrateing microfluid main entrance, microfluid passageway and microchannel in microfluid chip, adopts the micro-fluidic control technique to adhere the gathering with the separation of platelet, purification and platelet and carries out the integration.

4. The utility model provides a microfluid chip is through the aspect ratio of reasonable in design's microchannel for thereby the platelet receives big shearing force effect in the microchannel and is activated, further promotes the gathering of adhering of platelet.

Drawings

FIG. 1 is a schematic diagram of a microfluidic chip according to the present invention;

fig. 2 is a schematic structural view of the platelet function detection device of the present invention;

fig. 3 is a schematic structural diagram of a collagen control module in the platelet function testing device according to the present invention.

The same reference numbers will be used throughout the drawings to refer to the same or like elements or structures, wherein: a microfluidic chip 100; a blood injection port 101; a waste liquid collection port 102; a platelet separation and purification port 103; a drug injection port 104; a platelet waste fluid collection port 105; a first injection assembly 106; a second injection assembly 107; a third injection assembly 108; a fourth injection assembly 109; a microfluidic main channel 110; a fluorescent microscopic component 111; an upper substrate 112; a lower substrate 113; a microfluidic channel 114; a first conditioning microfluidic channel 115; a second conditioning microfluidic channel 116; a microchannel 120; a collagen control module 200; a first collagen microchannel 201; a second collagen microchannel 202; a fifth injection assembly 203.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Furthermore, the technical features mentioned in the embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

The blood sample in the utility model refers to the blood that contains whole blood and the plasma that is rich in platelets that is extracted from the human body.

Referring to fig. 1, the present invention provides a microfluidic chip, which includes a microfluidic main channel 110, a microfluidic channel 114, a microchannel 120, and a collagen control module 200. The microfluidic main channel 110, the microfluidic channel 114 and the microchannel 120 are in communication in sequence. The initial end of the microfluidic main channel 110 is provided with a blood injection port 101, and a first regulating microfluidic channel 115 and a second regulating microfluidic channel 116 are symmetrically provided on both sides in the direction of flow of the blood sample. The end of the microfluidic channel 114 is provided with a platelet separation and purification port 103 and a waste liquid collection port 102, wherein the waste liquid collection port 102 is used for collecting red blood cell and white blood cell waste liquid after blood sample separation, the platelet separation and purification port 103 is communicated with the microchannel 120, the microchannel 120 comprises a branch, the branch is provided with a drug injection port 104, and different drugs are injected from the drug injection port 104 to realize detection of platelet function inhibition under the action of different drugs. A collagen control module 200 is further disposed on the microchannel 120, and a platelet waste liquid collecting port 105 is disposed at the end of the microchannel 120.

Further, the micro fluid channel 114 is configured as a groove structure, the cross section of the groove structure of the micro fluid channel 114 is in any shape such as a Chinese character ' ao ', a U ', and the like, and a platelet separation effect with higher purity is achieved according to the size ratio relationship between platelets and white blood cells and red blood cells.

As a preferred embodiment of the present invention, the depth of the groove structure of the microfluidic channel 114 is 10 μm to 100 μm, and the width of the groove structure of the microfluidic channel 114 is 20 μm to 250 μm.

In the microfluid main channel 110, in order to reach the separation effect of obvious platelet and red blood cell, leucocyte, can realize through the velocity of flow and the stickness of fluid in first regulation microfluid passageway 115 and the second regulation microfluid passageway 116 of adjusting, for guaranteeing the separation effect, as the utility model discloses an preferred embodiment, high viscosity liquid is selected for use to the fluid in the first regulation microfluid passageway 115, and low viscosity liquid is selected for use to the second regulation microfluid passageway 116.

The utility model provides a microfluid chip obtains higher shearing force in microchannel 120 in order to obtain the platelet after the purification, and consequently the platelet is in but flow equivalence is the laminar flow of classic incompressible Newtonian fluid in the rectangle microchannel and flows in microchannel 120, microchannel 120 is provided with groove structure, microchannel 120 groove structure's cross sectional shape is arbitrary shapes such as character cut in bas-relief, U font. As a preferred embodiment of the present invention, the depth-to-width ratio of the trench structure of the microchannel 120 is 1: 10. As a further preferred embodiment, the width of the trench structure of the microchannel 120 is 240 μm.

Further, the microchannel 120 and the platelet separation and purification port 103 are integrally processed, and seamless connection can be realized.

In order to detect platelet inhibition by different drugs, a branch is provided at the microchannel 120, and a drug injection port 104 is provided at the branch.

In order to improve the adhesion of the platelet aggregation block, a collagen control module 200 is arranged at the microchannel 120, so that the adhesion test of collagen to platelets under different concentrations can be realized. As a preferred embodiment of the present invention, the concentration of the collagen is controlled to be between 100. mu.g/mL and 250. mu.g/mL.

The embodiment of the utility model provides an in the working process of microfluid chip does: a blood sample is injected at the blood injection port 101, separation of platelets from white blood cells and red blood cells in the blood sample is achieved by adjusting the flow rate of liquid injection of the first regulating microfluidic channel 115 and the second regulating microfluidic channel 116, and separation and purification of platelets are achieved through the platelet separation and purification port 103. The purified platelets flow through microchannel 120 to achieve activation of the platelets through the action of large shear forces. When the platelets flow through the collagen control module 200, the platelets are cohesively aggregated by interacting with the collagen. The control of the concentration and the injection rate of different drugs such as aspirin, clopidogrel and the like through the drug injection port 104 can realize the test of the platelet function inhibition under the action of different drugs.

Referring to fig. 2, another embodiment of the present invention provides a platelet function detecting device, which includes a microfluidic chip 100, an upper substrate 112, a lower substrate 113, a first injection assembly 106, a second injection assembly 107, a third injection assembly 108, a fourth injection assembly 109, and a fluorescence microscopic assembly 111, wherein the microfluidic chip 100 is completed by a technician on the upper substrate 112 except for a collagen control module 200.

A through hole is formed at the blood injection port 101 of the upper substrate 112 and an injection speed equal to a blood flow speed of a human body is achieved by the first injection assembly 106. Through holes are formed at the inlets of the second injection assembly 107 and the third injection assembly 108 of the upper substrate 112, the liquid flow rates of the first conditioning microfluidic channel 115 and the second conditioning microfluidic channel 116 can be realized through the second injection assembly 107 and the third injection assembly 108, and the separation of platelets can be realized through the action of soft inertia force. The drug injection port 104 of the upper substrate 112 is provided with a through hole and injects different drugs through the fourth injection assembly 109. Changes to platelet adhesion aggregation morphology and image analysis are achieved by the fluorescence microscopy component 111.

Referring to FIG. 3, the PFA-200 cannot accommodate the fluctuation of data and the defect of concentration of collagen-containing material in blood, resulting in the deviation of the detection result. The utility model discloses in collagen control module 200 includes first collagen microchannel 201, second collagen microchannel 202 and fifth injection subassembly 203, first collagen microchannel 201 with second collagen microchannel 202 is all in processing accomplishes on the lower floor's base plate 113. The microchannel 120 of the upper substrate 112 and the second collagen microchannel 202 of the lower substrate 113 are connected by the first collagen microchannel 201, wherein the first collagen microchannel 201 is filled with a hydrogel material. The first collagen microchannel 201 and the second collagen microchannel 202 are both processed on the lower substrate 113. A collagen injection port of the second collagen microchannel 202 of the lower substrate 113 is provided with a waste liquid collecting port for collagen to pass through and control the collagen concentration by the fifth injection assembly 203, and the tail end of the second collagen microchannel 202 is also provided with a waste liquid collecting port for collagen.

It should be noted that all the syringe assemblies are filled with the corresponding liquid before the detection starts, and each syringe assembly can input the corresponding speed through the electronic device to realize the liquid advancing speed at different speeds. Specifically, the first syringe assembly 106, the second syringe assembly 107, the third syringe assembly 108, the fourth syringe assembly 109 and the fifth syringe assembly 203 are each composed of a liquid storage container and a precision syringe pump and are communicated with each other through medical tubules.

In a preferred embodiment of the present invention, the material of the microfluidic chip is metal, glass, plastic or silicone. In addition, in order to satisfy the observation of the platelet adhesion and aggregation behavior in the blood sample and the image analysis, the microfluidic chip is preferably made of a transparent material. Furthermore, from the viewpoint of integration and convenience of processing, PDMS (polydimethylsiloxane) is preferred as a material for manufacturing the microfluidic chip, i.e., PDMS materials are used for the upper substrate 112 and the lower substrate 113. Because the PDMS material has excellent adhesiveness, the upper and lower PDMS substrates can be perfectly attached after plasma treatment, and the sealing property of the blood sample in the microchannel is ensured.

Furthermore, the micro-channels or through holes arranged on the upper and lower substrates of the microfluidic chip are formed by photoetching through a cutter or a laser beam, and the microfluidic chip can be manufactured by a molding method under the condition that the microfluidic chip is made of a PDMS material, wherein the manufacturing process mainly comprises the manufacturing of an SU-8 mold and the manufacturing of the PDMS microfluidic chip.

Further, it is necessary to perform anticoagulation treatment on a blood sample before the platelet function is measured. The anticoagulation agent used for anticoagulation may be sodium or potassium citrate, calcium oxalate, acidic citric acid, or the like. The anticoagulation agent preferably contains a sodium citrate solvent in an amount of 3.2%. The blood sample injected at the blood injection port 101 may be pre-anticoagulated prior to being filled into the first injection assembly 106. Heparin, hirudin, thromboplastin, etc. may also be used as an anticoagulation agent, and the anticoagulation agent may be a mixture of one or more of the above anticoagulation agents, for example, the anticoagulation agent hirudin, even without the treatment of a platelet activator, produces strong platelet aggregation under physical stimulation conditions and can be used in the shear force test platelet function test; a blood sample anticoagulated with citric acid can be used in an evaluation test of platelet-inhibiting drugs.

The fluorescence microscopy block 111 is embedded with a high speed camera and connected to an image analysis device by which the state of the platelet spark can be imaged. In addition, the fluorescent labeling of the platelets can be realized through the fluorescent dye Calcein AM, the fluorescent dye of living cells can penetrate through cell membranes, enter cells, and then is sheared by intracellular lipase to form Calcein and emit strong green fluorescence, the influence of the adhesion and aggregation of the fluorescent labeled platelets on the surface of collagen can be recorded through the fluorescent microscopic component 111, and a 300-chapter sequence fluorescent image can be obtained after 5 minutes of recording. The platelet adhesion and aggregation behaviors can be analyzed by using ImageJ software to acquire sequence images, and the surface coverage rate of the fluorescence labeling platelet aggregates in each image is calculated. Further, the index of surface coverage quantification may be quantified by an algorithm of image processing as a result of data observation.

Through the utility model discloses platelet function detection device is to platelet function detection method:

The technical solution of the present invention is further described below by specific examples, and the detection of platelet function of the present invention specifically includes:

s1, two transparent substrates are prepared.

Preferably, the material of the transparent substrate is a PDMS material.

Specifically, the two transparent substrates are an upper substrate 112 and a lower substrate 113. In the upper substrate 112, the microfluidic channel 114 has a length of 30mm, a depth of 70 μm, and a width of 1.5 mm. The microchannel 120 has a length of 20mm, a depth of 70 μm, and a width of 700 μm. The first collagen microchannel 201 has a depth of 150 μm and a width of 500 μm, wherein the first collagen microchannel 201 is filled with hydrogel.

S2, anticoagulating the blood sample collected from human body with hirudin (20 μ g/mL), and fluorescence labeling the platelet with Calcein AM fluorescent dye, and injecting into the first injection assembly 106.

Specifically, the anticoagulated blood sample flows through the microfluidic channel 114, and the two-stage pressure difference generated by the sheath flow generated by the second injection assembly 107 and the third injection assembly 108 causes the particles in the shear flow to shift, thereby realizing separation and enrichment of platelet separation.

S3, the concentration of the separated and enriched platelets flowing into the hydrogel-filled first collagen microchannel 201 is controlled by the fifth injection assembly 203 while flowing through the microchannel 120 of the collagen control module 200.

Specifically, hydrogel in the first collagen microchannel 201 can realize the rate of collagen permeation into the microchannel 120, thereby realizing the rate of collagen binding to platelets.

S4, when the platelets flow through the first collagen microchannel 201, the platelets can rapidly adhere and aggregate by interacting with collagen.

Specifically, the state of platelet activation can be imaged by connecting the fluorescence microscopy unit 111, which has a high-speed camera embedded therein, to an image analysis device. The influence of the adhesion and aggregation of the fluorescence labeled platelet on the surface of the collagen can be recorded by a fluorescence microscope, and a 300-chapter sequence fluorescence image can be obtained after recording for 5 minutes. The platelet adhesion and aggregation behavior can be analyzed by using ImageJ software to acquire sequence images, and the surface coverage rate of the fluorescence labeling platelet aggregates in each image and a thrombus three-dimensional image are calculated. The indicator of surface coverage quantification may be quantified by an algorithm of image processing as a result of data observation. The dynamic model of platelet adhesion and aggregation can judge the functions of the platelets.

It will be understood by those skilled in the art that the foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

Claims

1. A microfluidic chip, characterized in that the microfluidic chip (100) comprises: a microfluidic main channel (110) and a microfluidic channel (114);

the end of the microfluidic main channel (110) communicates with the microfluidic channel (114);

the microfluidic main channel (110) is provided with a blood injection port (101), a first regulating microfluidic channel (115) and a second regulating microfluidic channel (116); the blood injection port (101) is arranged at the initial end of the micro-fluid main channel (110); the first adjusting microfluidic channel (115) and the second adjusting microfluidic channel (116) are oppositely arranged at two sides of the microfluidic flow direction and are respectively used for injecting first adjusting liquid and second adjusting liquid with different flow rates;

the end of the micro-fluid channel (114) is provided with a platelet separation and purification port (103) and a waste liquid collection port (102).

2. A microfluidic chip according to claim 1, wherein the microfluidic channel (114) is provided as a trench structure for adhesive aggregation of platelets separated from a blood sample.

3. The microfluidic chip according to claim 1 or 2, wherein the microfluidic chip (100) further comprises a microchannel (120), an inlet of the microchannel (120) is communicated with the platelet separation and purification port (103), and an outlet of the microchannel is provided with a platelet waste liquid collection port (105); the micro-channel (120) is used for enabling the flow of the platelets in the micro-channel (120) to be equivalent to the laminar flow of the incompressible Newtonian fluid in the rectangular micro-channel, so that the purified platelets can be self-activated under the action of shear force.

4. A microfluidic chip according to claim 3, wherein said microchannels (120) are arranged in a trench structure.

5. The microfluidic chip according to claim 4, further comprising a drug injection port (104), wherein the drug injection port (104) is in communication with the microchannel (120).

6. The microfluidic chip according to claim 5, further comprising a collagen control module (200), wherein the collagen control module (200) is disposed at the microchannel (120).

7. A platelet function testing device, characterized in that it comprises a microfluidic chip (100) according to claim 6, further comprising: an upper substrate (112), a lower substrate (113), a first injection assembly (106), a second injection assembly (107), a third injection assembly (108), and a fourth injection assembly (109);

the upper substrate (112) is coupled with the lower substrate (113), the microfluidic main channel (110), the microfluidic channel (114) and the microchannel (120) are arranged on the upper substrate (112), and the collagen control module (200) is arranged on the lower substrate (113);

the first injection assembly (106) is in communication with the blood injection port (101);

the second injection assembly (107) is in communication with the second conditioning microfluidic channel (116);

the third injection assembly (108) is in communication with the first conditioning microfluidic channel (115);

the fourth injection assembly (109) is in communication with the drug injection port (104).

8. The platelet function testing device according to claim 7, wherein the collagen control module (200) comprises a first collagen microchannel (201) and a second collagen microchannel (202) which are positioned on the lower substrate (113) and are communicated with each other, and a fifth injection assembly (203) which is communicated with the second collagen microchannel (202); the first collagen microchannel (201) is positioned above the second collagen microchannel and is in communication with the microchannel (120); the fifth injection assembly (203) is used for injecting collagen liquid into the second collagen micro-channel (202) and controlling the concentration of the collagen liquid.

9. The platelet function testing device according to claim 8, further comprising a fluorescence microscopy component (111), wherein the fluorescence microscopy component (111) is disposed above the upper substrate (112) and focused on the microchannel (120).