CN212770762U - Organ chip system for biological reaction - Google Patents

Abstract

The utility model aims to solve the technical problem that an organ chip system for biological reaction is provided, including main control jar, cultivation unit, liquid storage pot and waste liquid jar absorb exhaust waste material to main control jar transported substance material and from main control jar respectively, and main control jar carries the culture solution of adjusting through the main control jar respectively to cultivateing the unit for carry out biological reaction or cultivate in cultivateing the unit. The utility model discloses utilize general thought and the principle of organ chip, constructed one set and can realize changing liquid and real time monitoring's biological culture apparatus voluntarily, the structure is convenient moreover, and maneuverability is strong, and the popularization is wide.

Description

Organ chip system for biological reaction

Technical Field

The utility model relates to the field of biotechnology, especially, relate to an organ chip system for biological cultivation.

Background

Biological culture is widely applied to the field of biotechnology, and at present, a popular method is to introduce the concept of organ chips in biological culture to enable the biological culture to realize automation and intellectualization, but most organ chips cannot realize popularization due to high manufacturing cost and complex flow.

Disclosure of Invention

The utility model aims to solve the technical problem that an organ chip system for biological reaction is provided, its big notion and the principle that utilize the organ chip have constructed one set of biological culture apparatus that can realize changing liquid and real time monitoring voluntarily, and it is convenient to construct moreover, and maneuverability is strong, and the popularization is wide.

The utility model provides a technical scheme that above-mentioned technical problem adopted is:

the utility model provides an organ chip system for biological reaction, includes main control jar, cultivates unit, liquid storage pot and waste liquid jar, and liquid storage pot and waste liquid jar carry the material respectively to the main control jar and absorb the waste material of discharge from the main control jar, and the main control jar carries the culture solution that passes through the main control jar regulation respectively to cultivateing the unit for carry out biological reaction or cultivate in cultivateing the unit.

Further, the organ chip system further comprises a main control room, the main control tank is arranged in the main control room, and a temperature adjusting device is arranged in the main control tank or the main control room.

Further, the main control tank or the main control chamber is internally provided with at least one of a PH sensor and an oxygen sensor.

Furthermore, a stirring device is arranged in the main control tank and can be used for stirring to adjust the culture solution in the main control tank. In some preferred embodiments, a magnetic stirring device may be used to save space, but other stirring devices may be used for stirring purposes, and the stirring device is generally required not to damage cells.

Furthermore, the main control tank is provided with an air inlet device, and the air inlet device can introduce or respectively introduce at least CO into the main control tank₂、N₂、O₂The gases contained in the reaction mixture may be introduced separately or in a mixture. In some preferred modes, a neutralization solution such as sodium bicarbonate solution can be introduced into the main control tank to adjust the pH value in the culture medium.

Further, the culture units are arranged in a certain order or rule. Preferably, the culture unit can be connected in series to the liquid supply circuit, preferably, the culture unit can be connected in parallel to the liquid supply circuit, or in some other cases, the liquid supply unit can be connected in series or in parallel to the liquid supply circuit.

Further, the device comprises a control unit, the control unit can receive signals of various sensors arranged on the main control tank and send control signals to the feeding and discharging system according to the signals, automatic liquid changing and real-time monitoring of the organ chip system are realized through the control unit, preferably, the control unit can adopt a host, a server or a single chip microcomputer and the like, the control unit is used for receiving signals, processing signals and feeding back signals, for example, an oxygen sensor is arranged in the main control tank or a main control room and monitors the concentration of oxygen in the main control tank in real time, when the concentration of oxygen reaches a certain concentration, the input quantity of oxygen is reduced by the main control tank, when the concentration of oxygen is lower than the certain concentration, the input quantity of oxygen is increased by the main control tank, so that the concentration of oxygen in the main control tank is adjusted, the concentration of oxygen in the main control tank is determined according to the requirement of culture, for example, different oxygen-containing environments may be required for different biological cultures, for example, some anaerobic bacteria may be cultured, in which case the oxygen input may be reduced, and if aerobic bacteria are cultured, the oxygen input may be increased, and other gases, the temperature in the main control tank and the PH of the culture solution in the main control tank may all be adjusted in a similar manner, and similarly, whether the magnetic stirring device stirs or not and the stirring speed may also be adjusted by the control unit, and accordingly, the control unit may adjust the stirring speed of the magnetic stirring device according to the information of the sensor, or set according to the experimental requirements.

Further, the utility model discloses specific culture unit can be adopted, for example, the utility model discloses a culture unit can include cultivates the chamber, cultivate the chamber and include at least one deck diaphragm, the diaphragm can with cultivate the chamber and keep apart for two at least cultivation regions, the diaphragm sets up in cultivateing the intracavity usually to can be at certain position relatively fixed in cultivateing the intracavity.

Further, the membrane sheet comprises a semi-permeable membrane, and the membrane sheet can be a semi-permeable membrane, a fully permeable membrane or an impermeable membrane, and can also be a combination of the membranes.

Furthermore, the membrane is provided with a plurality of through holes which are distributed on the membrane, and the aperture of each through hole can be 30 μm-1mm, so that the membrane can form a semi-permeable membrane state, can allow a part of substances to permeate through the membrane, and simultaneously block a part of substances.

Further, the culture chamber adopts a biosafety container and/or a biochip. In some modes, the culture cavity can adopt a container meeting the biosafety requirement, such as a glass culture dish, a centrifuge tube and the like, and in some modes, the culture cavity can adopt a biochip which is more commonly used at present, so that the culture cavity is better in adaptability.

Further, the culture regions can be independent or in communication with each other. In some embodiments, the membranes may be impermeable to separate the culture regions to form separate culture chambers, in other embodiments, the membranes may be semi-permeable to partially separate the culture regions, or in other embodiments, the membranes may be permeable to reduce the flow rate of the culture medium or culture medium through the pores of the membranes.

Further, the culture cavity comprises a liquid inlet and a liquid outlet, and can be used for inputting and outputting culture solution and/or culture substances.

Further, the culture cavity includes the liquid taking port, and in some modes, can get the liquid through the liquid taking pipe, then the liquid taking port can include the liquid taking pipe this moment, and the liquid taking port can stretch into the culture cavity for sampling when carrying out the cultivation etc. in some modes, the liquid taking port can directly set up on certain cultivation region.

Further, the membrane comprises a membrane support for supporting, the membrane support can be used for supporting and fixing the membrane, the membrane support can also be made of a biological safety material, such as biological safety plastic or plastic material, and the like, the membrane support can clamp and fix the membrane, and then the membrane support is further fixed on the inner wall of the culture cavity, so that the membrane is fixed.

Further, the membrane support comprises at least one area for installing the membrane, and in some modes, the membrane support can be divided into a plurality of areas, so that on one hand, the fixation of the membrane can be reinforced, on the other hand, the expansibility of the membrane can be better, for example, different types of membranes can be respectively placed in different areas of the membrane support to be matched for use, and the effect of comprehensive utilization can be achieved.

Further, the culture cavity comprises a blocking component which is used for closing the culture cavity to form a closed culture space, so that pollution is avoided, and liquid leakage is prevented.

The utility model has the advantages that:

(1) the utility model discloses an organ chip system has set up main control jar and cultivation unit, adjusts suitable state with culture solution or cultivation environment in the main control jar, inputs the cultivation unit again, simultaneously, can set for the temperature in culture apparatus, ensures the stability of cultivateing the condition.

(2) The utility model discloses an organ chip system passes through liquid storage pot, master control jar, cultivates unit and waste liquid jar, has formed complete culture solution circulation circuit, combines the control unit, introduces power module, for example the pump, can realize the automatic liquid changing of culture solution.

(3) The utility model discloses an organ chip system has set up a plurality of sensors in the main control jar, corresponds different cell culture demands respectively, and various parameters of cell culture liquid in the main control jar are gathered in real time to these sensors to feedback the control unit, the control unit can be adjusted the volume of each parameter in the organ chip system according to the state of real-time feedback, realizes the automatic control of process of cultivateing.

(4) The utility model also provides a special culture unit, this culture unit device can regard as the independent culture unit who is used for cultivateing in the organ chip system, can also set up at least one deck in this culture unit device and cultivate the diaphragm, will cultivate the chamber and keep apart into different cavities for cultivate respectively or compare the cultivation.

Drawings

Fig. 1 is a schematic diagram of the overall architecture of the present invention.

Fig. 2 is a basic structure schematic diagram of the main control tank of the present invention.

Fig. 3 is a control schematic diagram of the present invention.

FIG. 4 is a schematic view of the overall structure of a first culture unit of the present invention.

FIG. 5 is a schematic view of the first holder ring of the first culture unit of the present invention.

FIG. 6 is a schematic view of a second frame ring of the first culture unit of the present invention.

FIG. 7 is a schematic view of a fixing ring of the first culture unit of the present invention.

FIG. 8 is a partially enlarged view of a membrane sheet of the first culture unit of the present invention.

Fig. 9 is an overall structural schematic diagram of the embodiment.

FIG. 10 is a schematic view of the entire structure of embodiment 3.

FIG. 11 is a schematic view of the entire structure of embodiment 4.

Reference numbers in the figures: a culture unit 1, a main control tank 100, a culture area 101, a liquid storage tank 112, a waste liquid tank 113, a sensor 114, a magnetic stirring device 115, an air inlet device 116, a main control chamber 200, a culture chamber 201 and a liquid storage chamber 202; the device comprises a plugging part 2, a liquid inlet pipe 3, a liquid outlet pipe 4, a sampling pipe 5, a diaphragm 6, a via hole 61, a pipe hole 7, a support ring 8, a fixing ring 9, a pipe support 10, a support frame 11, a pipe hole part 12, a support part 13 and a gap 14; a liquid inlet layer 17, a liquid inlet 171, a culture layer 18, a liquid outlet layer 19 and a liquid outlet 191; a liquid inlet 15, a liquid outlet 16, a first culture area 102, a second culture area 103 and a third culture area 104; first culture vessel 106, second culture vessel 107, third culture vessel 108, and cover plate 109.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings, and it should be noted that the embodiments are only for illustrating the present invention in detail, and the purpose thereof is to enable those skilled in the art to better understand the technical solution of the present invention, and should not be construed as limiting the present invention.

As shown in FIG. 1, the utility model provides an organ chip system for biological reaction, including main control jar 100, cultivation unit 1, liquid storage pot 112 and waste liquid jar 113, liquid storage pot 112 is to main control jar 100 transported substance material, and waste liquid jar 113 carries out simple purification treatment from main control jar absorption exhaust waste material respectively, and main control jar 100 carries the culture solution through main control jar 100 regulation respectively to cultivation unit 1 for carry out biological reaction or cultivate in cultivation unit 1.

The organ chip system further comprises a main control chamber 200, a culture chamber 201 and a liquid storage chamber 202, wherein the main control tank 100 is arranged in the main control chamber 200, the culture unit 1 is arranged in the culture chamber 201, a plurality of culture units 1 can be arranged in the culture chamber 201, the liquid storage tank 112 and the waste liquid tank 113 are arranged in the liquid storage chamber 202, the main control tank 100, the culture unit 1, the liquid storage tank 112 and the waste liquid tank 113 are connected through a liquid conveying pipeline 300, and the liquid conveying pipeline 300 can penetrate through a partition plate 400 for isolating the main control chamber 200, the culture chamber 201 and the liquid storage chamber 202, so that a liquid supply loop of the liquid storage tank 112 → the main control tank 100 → the culture unit 1 → the main control tank 100 → the waste liquid tank 113 is formed.

Preferably, the plurality of culture units 1 may be arranged in a certain order or in a regular manner. For example, the plurality of culture units 1 may be connected in series to the liquid supply circuit as shown in fig. 1, in other cases, the culture units 1 may be connected in parallel to the liquid supply circuit, or in other cases, the culture units 1 may be connected in series or in a parallel combination to the liquid supply circuit, for example, two of the culture units 1 are connected in series, and the other two are connected in parallel, and the specific connection mode may be selected according to the needs of the culture.

As shown in FIG. 2, the master tank 100 is provided with various sensors 114, such as O₂Sensor or CO₂Sensor or N₂The sensor for the concentration or the equivalent of all kinds of gas in the sensing main control jar 100, still be equipped with temperature sensor on the main control jar 100 for the temperature in the sensing main control jar 100, still be equipped with the pH value sensor on the main control jar 100 for the pH value of the interior culture solution of sensing main control jar 100.

Preferably, a magnetic stirring device 115 may be disposed in the main control tank 100, and may be used for magnetic stirring to adjust the culture solution in the main control tank 100, so as to facilitate the fusion of the gases.

Preferably, the main control tank 100 is provided with an air inlet device 116, and the air inlet device can introduce or respectively introduce at least CO into the main control tank₂、N₂、O₂The gases can be introduced through different pipelines respectively or can be introduced through a pipeline in a mixed mode.

Preferably, a sodium bicarbonate solution may be placed in the waste liquid tank 113 to purify the used waste liquid and lower the PH thereof, thereby minimizing contamination.

As shown in fig. 3, the organ chip system further includes a control unit 500, the control unit 500 can receive signals of various sensors disposed on the main control tank 100, and send control signals to the feeding and discharging system according to the signals, the automatic liquid change and real-time monitoring of the organ chip system can be realized by the control unit, preferably, the control unit can adopt a host, a server or a single chip, etc., the control unit is used for receiving the signals, processing the signals and feeding back the signals, for example, an oxygen sensor is disposed in the main control tank 100 or the main control room 200, the oxygen sensor monitors the oxygen concentration in the main control tank in real time, when the oxygen reaches a certain concentration, the main control tank reduces the input amount of the oxygen, when the oxygen is lower than the certain concentration, the main control tank increases the input amount of the oxygen, thereby adjusting the oxygen concentration in the main control tank, the oxygen concentration in the main control tank is determined according to the requirement of the culture medium, for example, different oxygen content environments may be required for different biological cultures, for example, some anaerobic bacteria cultures, in which case the oxygen input may need to be reduced, if aerobic bacteria cultures, the oxygen input may need to be increased, other gases, the temperature in the main control tank and the PH value of the culture solution in the main control tank may all adopt similar adjustment modes, and similarly, whether the magnetic stirring device stirs or not and the stirring speed may also be adjusted by the control unit, and accordingly, the control unit may also adjust the stirring speed of the stirring device according to the information of the sensor, or set according to the requirement of the experiment.

It should be noted that the various sensors and control devices of the present invention can all adopt the existing models in the prior art, the present invention does not improve the software or the control principle, and the present invention improves the sensors and control methods in the prior art to be applied to the culture device.

On this basis, the utility model discloses still provide an improved generation culture unit, this culture unit can be used for the utility model discloses an organ chip system also can independently be used for other culture apparatus, as one of culture unit.

The culture unit used in the present invention will be described in detail below.

First culture unit: a centrifugal tube culture unit.

As shown in fig. 4-8, as shown in the figures, the cultivation unit 1 of the present invention can be a centrifuge tube as shown in the figures, or a common test tube or a similar test device with a certain diameter, and the cultivation unit 1 can be a transparent tube made of glass or plastic material.

Cultivate the oral area of unit 1 and be equipped with plugging member 2, plugging member 2 can adopt cork or rubber buffer, be equipped with on plugging member 2 and stretch into cultivate the inside pipe of unit 1, the pipe includes feed liquor pipe 3, drain pipe 4 and sampling tube 5, feed liquor pipe 3 stretches into the position that is close to the bottom of cultivateing unit 1, and generally speaking, feed liquor pipe 3 is close the bottom better more, but does not touch the bottom, will leave 1-5mm play liquid distance, avoids blockking up, the oral area of drain pipe 4 is close to the bottom of plugging member 2, is close the position of the last liquid level of culture solution, generally speaking, perhaps breaks the certain time after the cultivation is accomplished, carries out the flowing back.

The sampling tube 5 extends into the middle of the culture unit 1 for extracting culture material.

In the culture unit 1, a membrane 6 is provided, and in this way of using a centrifuge tube as the culture unit 1, preferably, the membrane 6 is arranged perpendicular to the height direction of the culture unit 1, and simply, the membrane 6 can be placed transversely in the culture unit 1 to separate the culture unit 1 into different culture spaces. Preferably, an impermeable membrane, a semi-permeable membrane or a fully permeable membrane can be placed on the membrane, and the membrane can be selected as required, when the fully permeable membrane is placed, the culture unit 1 can be divided into different culture areas 101, in some cases, scales can be arranged on the wall of the culture unit 1, the placement position of the membrane 6 can be adjusted, and the culture space can be determined according to the scales; when the semipermeable membrane is placed, selective permeation can be realized, and culture of different levels can be realized; when the perm-membrane is placed, the flow rate of the culture solution into the next culture space can be adjusted. For example, as shown in fig. 5, a plurality of through holes 601 may be disposed on the membrane 6, and the through holes 601 may be distributed on the membrane 6, and the aperture of the through holes 601 may be 30 μm to 1mm, so that the membrane 6 may form a semi-permeable membrane state, which can allow a part of the substance to permeate therethrough, and block a part of the substance.

Preferably, the membrane 6 may be supported by a membrane holder, the membrane holder includes a holder ring 8 and a fixing ring 9, the holder ring 8 is made of plastic or plastic material, the holder ring 8 includes a tube holder 10 and a support frame 11, the tube holder 10 is provided with the at least one tube hole 7, the support frame 11 is in accordance with the shape of the inner wall of the culture unit 1, for example, when the culture unit 1 has a circular cross section, the support frame 11 has a circular shape, when the culture unit 1 has a square shape, the support frame 11 has a square shape, so as to ensure that the support frame 11 can be supported on the cross section of the culture unit 1, and the periphery of the support frame 11 may be sleeved with a sealing ring for fixing and sealing the periphery.

Preferably, the pipe bracket 10 includes a pipe hole portion 12 and a bracket portion 13, the pipe hole 7 is provided in the pipe hole portion 12, and a sealing ring may be fitted around a pipe passing through the pipe hole 7 to seal a gap between the pipe hole 7 and an outer wall of the pipe to prevent leakage. The support parts 13 are diverged or uniformly distributed between the tube hole part 12 and the support frame 11 for connection of the two, for example, in the culture unit, the support parts 13 may be configured as a cross-shaped support and uniformly distributed in the space between the tube hole part 12 and the support frame 11, accordingly, a gap 14 is formed between the support parts 13, and this gap 14 may be filled with the above-mentioned impermeable film, semi-permeable film or fully permeable film, for example, when the support is manufactured, the impermeable film, semi-permeable film or fully permeable film is adhered to the support parts 13 and the support frame 11, and in some ways, different types of membranes may be filled in different gaps 14 according to needs.

Preferably, the fixed ring 9 can be made of silica gel materials, the fixed ring 9 can be sleeved on the periphery of the support frame 11, the fixed ring made of silica gel materials can be adsorbed on the inner wall of the culture unit through friction force, so that the membrane is fixed on the inner wall of the culture unit, in some preferred modes, the fixed ring 9 is fixed on the periphery of the support frame 11 through self elastic force, in some preferred modes, the upper end and the lower end of the periphery of the support frame 11 can be provided with bulges, and the fixed ring 9 is limited in the upper end and the lower end.

Preferably, as shown in FIG. 4, the membrane sheet 6 may include two sheets, and when the membrane sheet is two sheets, they are disposed in the culture unit 1 at a certain distance, and the culture unit 1 may be partitioned into more spaces.

Preferably, generally, there are more through holes on the upper membrane than on the lower membrane, for example, when there are two through holes on the upper membrane, the lower membrane may have only one through hole, the two through holes of the upper membrane may be used for passing through the liquid inlet pipe and the sampling pipe, and the through hole of the lower membrane only needs to pass through the liquid inlet pipe, in this case, we may refer to the bracket ring in the upper membrane as the first bracket ring, there are two pipe holes 7 on the through hole portion 13 of the first bracket ring for passing through the liquid inlet pipe 3 and the sampling pipe 5, we may refer to the bracket ring in the lower membrane as the second bracket ring, there is one pipe hole 7 on the through hole portion 13 of the second bracket ring for passing through the sampling pipe 5. Of course, the utility model discloses also do not get rid of the condition of a plurality of feed liquor pipes, a plurality of sampling tubes, multilayer diaphragm, can set up as required, for example can adopt a plurality of sampling tubes, stretch into respectively and take a sample in the middle of the different cultivation layers.

A second culture unit: a biochip is provided.

As shown in fig. 9, the culture unit 1 of the present invention can be a biochip, which comprises a liquid inlet layer 17, a culture layer 18 and a liquid outlet layer 19, wherein the culture layer 18 is located between the liquid inlet layer 17 and the liquid outlet layer 19, wherein the liquid inlet layer 17 is provided with a plurality of liquid inlets 171 (e.g., 3 in the figure), the liquid inlet layer 171 can be used for culturing the liquid in the chip, the liquid outlet layer 19 is provided with at least one liquid outlet 191 (e.g., 2 in the figure), the liquid inlet layer 17, the culture layer 18 and the liquid outlet layer 19 can be provided with a membrane 6, and the membrane 6 can be free of a membrane support in the culture unit.

Further, in this culture unit, other structures may be adopted similar to those of the first culture unit, such as the structure of the membrane 6 and the like.

A third culture unit: a biosafety container.

As shown in FIG. 10, the culture unit 1 may be a container made of biosafety material, the volume of the container may be 10. mu.L-500 mL, the upper portion of the container is provided with a liquid inlet 15, the lower portion of the container is provided with a liquid outlet 16, and in some other ways, the positions of the liquid inlet 15 and the liquid outlet 16 may be interchanged according to the connection manner of the liquid inlet and the liquid outlet.

Three different culture areas 101 are arranged in the container, the culture areas 101 can be separated by membranes 6, the liquid inlet 15 is communicated with a first culture area 102, the first culture area 102 is cylindrical as a whole and has a smaller inner diameter, preferably, a layer of membrane 6 can be arranged between the liquid inlet 15 and the first culture area 102 for filtering or separation, preferably, a transition area with gradually-increased caliber is arranged between the liquid inlet 15 and the first culture area 102 for introducing liquid, the first culture area 102 is respectively communicated with a plurality of (3 in the example shown) second culture areas 103 in different directions after one section, the membranes 6 can be arranged between the second culture areas 103 and the first culture area 102 for separating different areas, and the membranes 6 can be selected from semipermeable membranes, impermeable membranes or completely permeable membranes according to requirements, a third culture area 104 is arranged below the second culture area 103, a plurality of second culture areas 103 can be structurally communicated with the third culture area 104, a membrane 6 can be arranged at the junction of the second culture areas 103 and the third culture areas 104, similarly, the membrane 6 can also be a semi-permeable membrane, an impermeable membrane or a full-permeable membrane according to needs, and the lower part of the third culture area 104 is communicated with the liquid outlet 16, thus forming the integral structure of the whole culture unit 1.

Also, in this culture unit, the mounting manner of the membrane 6 can be realized as in the first culture unit, and the culture unit can be laid horizontally, vertically, or obliquely as needed, and is not limited to the direction shown in fig. 7.

A fourth culture unit: another biosafety container.

As shown in FIG. 11, the culture unit 1 may be a container made of a biosafety material, the container may have a volume of 10. mu.L-500 mL, the container may include a plurality of culture regions independent of each other, for example, a split design, the container may include a first culture container 106 at the bottom, a second culture container 107 capable of being accommodated in the first culture container 106, and a third culture container 108 on the second culture container 107, the second culture container 107 and the third culture container 108 may be connected integrally and internally, and a cover plate 109 may be provided outside the second culture container 107 and the third culture container 108, and when the second culture container 107 is put into the first culture container 106, the cover plate 109 may cover the mouth of the first culture container 106 to close the first culture container 106.

Furthermore, the first culture vessel 106 and the third culture vessel 108 are provided with a liquid inlet 15 and a liquid outlet 16, respectively, and the liquid inlet 15 and the liquid outlet 16 are separated from the culture spaces of the first culture vessel 106 and the third culture vessel 108 by a membrane 6, respectively.

In this culture unit, the membrane 6 may have the same inner diameter as the inlet port 15 and the outlet port 16, and the membrane 6 may be constructed as in the first culture unit.

It should be noted that the above culture units are only for listing the structures of some culture units that can be applied to the differences of the present invention, and are not shown, the present invention is limited to the above culture units, and in fact, the present invention can also be realized by selecting other existing or specific culture units according to actual needs, which does not affect the overall function of the present invention.

Similarly, the embodiments are also intended to enable those skilled in the art to better understand the technical solution of the present invention, and should not be considered as limiting the present invention, it should be noted that the embodiments of the present invention can be applied in other ways besides the way described in the embodiments, and all the scope covered by the claims of the present invention should be considered as the protection scope of the present invention.

Claims (10)

1. The utility model provides an organ chip system for biological reaction, characterized by includes main control jar, cultivates unit, liquid storage pot and waste liquid jar, and liquid storage pot and waste liquid jar carry the material respectively to the main control jar and absorb the waste material of discharge from the main control jar, and the main control jar carries the culture solution that passes through the main control jar regulation respectively to cultivateing the unit for carry out biological reaction or cultivate in cultivateing the unit.

2. The organ chip system for biological reaction according to claim 1, comprising a main control room, wherein the main control tank is disposed in the main control room, and a temperature adjusting device is disposed in the main control tank or the main control room.

3. The organ chip system for biological reaction according to claim 2, wherein at least one of a PH sensor and an oxygen sensor is provided in the main control tank or the main control chamber.

4. The organ chip system for biological reaction according to claim 1, wherein a stirring means is provided in the main control tank.

5. The organ chip system for biological reaction according to claim 1, wherein the main control tank is provided with an air inlet device, and the air inlet device can introduce or separately introduce at least CO into the main control tank₂、N₂、O₂The gas inside.

6. The organ chip system for biological reaction according to claim 1, wherein the culturing units are arranged in a certain order or a regular arrangement.

7. The organ chip system for bioreaction according to claim 1, comprising a control unit capable of receiving signals from various sensors provided on the main control tank and transmitting control signals to the feeding and discharging system according to the signals, thereby realizing automatic liquid change and real-time monitoring of the organ chip system by the control unit.

8. The organ chip system for biological reaction according to claim 1, comprising a culture chamber, wherein the culture chamber comprises at least one membrane capable of isolating the culture chamber into at least two culture areas.

9. The organ-chip system for biological reaction according to claim 8, wherein the membrane includes a semi-permeable membrane, and the membrane may be provided with a via hole.

10. The organ chip system for biological reaction according to claim 8, wherein the culture chamber employs a biosafety container and/or a biochip.

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