CN210869621U - Spiral large-scale immune cell vitrification cryopreservation tube - Google Patents
Spiral large-scale immune cell vitrification cryopreservation tube Download PDFInfo
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- CN210869621U CN210869621U CN201921500165.XU CN201921500165U CN210869621U CN 210869621 U CN210869621 U CN 210869621U CN 201921500165 U CN201921500165 U CN 201921500165U CN 210869621 U CN210869621 U CN 210869621U
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Abstract
The utility model provides a spiral extensive immune cell vitrification freezes deposits pipe, including spiral pipe, metal strip and two-dimensional code label, the spiral pipe is spiral pipe, and the spiral pipe is provided with import and export, and imports and export and set up respectively at the both ends of spiral pipe, the two-dimensional code label sets up at the entry end for information such as sign identity, the metal strip is installed in the both sides of spiral pipe, plays support and heat conduction effect. The utility model discloses simple structure, convenient to use has increased area of contact through the design of spiral pipe, improves and freezes and deposits efficiency, can once only realize large-scale vitrification freezing immune cell.
Description
Technical Field
The utility model relates to an apparatus technical field for the medical science especially relates to a spiral cryopreserving pipe that is used for the large-scale vitrification of immune cell to freeze.
Background
The adoptive immunotherapy has achieved certain results for treating various diseases, and the goal of preserving immune cells at different stages before the disease is not encountered to treat possible diseases in the future is the trend, and the preservation of immune cells to ensure the stability of immune cells and the permanent use of cellular materials, and the establishment of efficient large-scale freezing for preservation is needed.
The vitrification freezing method is a new freezing technique developed in recent years, and the vitrification freezing technique uses a freezing bearing tool with tiny volume to change a high-concentration freezing protection liquid from a liquid state to a solid state with high viscosity and similar to a glass sample in a very quick cooling process, and the liquid state is stored for a long time at low temperature. The ultra-fast cooling rate can avoid the formation of ice crystals in cells, and greatly reduces the cell damage in the freezing process. The vitrification cryopreservation method can keep the activity and the integrity of original cells and can reduce the antigenicity of the original cells, the components and the formula of the cryoprotectant can be used for preserving tissues and organs of a human body, and the human sperms, oocytes, embryos, erythrocytes, embryonic stem cells, hematopoietic stem cells, pancreatic islets and the like are successfully preserved so far. However, vitrification freezing has defects, the traditional vitrification freezing tube can only freeze 2-5 × 106 cells at a time, the storage amount is small, and the vitrification freezing of large-scale immune cells is obviously not facilitated.
Disclosure of Invention
For solving prior art's above-mentioned not enough, the utility model provides a spiral extensive immune cell vitrification freezes deposits pipe, including spiral pipe, metal strip and two-dimensional code label, the spiral pipe is spiral pipe, and the spiral pipe is provided with import and export, and import and export and set up respectively at the both ends of spiral pipe, the two-dimensional code label sets up at the entry end for information such as sign identity, the metal strip is installed in the both sides of spiral pipe, plays support and heat conduction effect. The utility model discloses simple structure, convenient to use has increased area of contact through the design of spiral pipe, improves and freezes and deposits efficiency, can once only realize large-scale vitrification freezing immune cell.
The utility model discloses a concrete technical scheme as follows:
the utility model provides a spiral large-scale immune cell vitrification freezes deposits pipe, vitrification freezes deposits the pipe and includes spiral pipe, metal strip and two-dimensional code label, the spiral pipe is resistant dark low temperature capillary, and it includes import and export, import and export set up respectively the both ends of spiral pipe, the metal strip is installed the both sides of spiral pipe, the two-dimensional code label sets up the entry end of spiral pipe.
The spiral pipe is easy to break after being solidified, the metal strip is arranged on two sides of the spiral pipe to fix the spiral pipe, plays a supporting role, prevents the spiral rubber pipe from being wound, enables the spiral pipe to be smoothly immersed in liquid nitrogen for freezing, has a heat conduction function, enables heat to be quickly transmitted, is used for identifying information such as identity, and immune cells are injected through an inlet of the spiral pipe and are sucked through an outlet of the spiral pipe. The spiral structure can greatly increase the one-time freezing quantity.
Further, the pipe diameter of the spiral pipe is 1 mm-5 mm, preferably 2 mm.
Further, the diameter of the spiral pipe is 10 mm-50 mm, and preferably 30 mm.
Furthermore, the inlet and the outlet of the spiral tube are thermally sealed and frozen, and when cells need to be used, the seal is removed after recovery.
Furthermore, the spiral tube is made of polypropylene materials, can resist the ultralow temperature of 196 ℃ below zero, is light in weight and can be rapidly cooled.
Further, the metal strip is bonded with the spiral pipe through glue, and the glue is epoxy resin glue.
Further, the two-dimensional code label is fixed on the metal strip, for example, adhered to the metal strip by glue.
Furthermore, the diameter of the vitrification cryopreservation tube is gradually reduced from top to bottom, and the lower end of the vitrification cryopreservation tube is smaller than the diameter of the upper end of the vitrification cryopreservation tube, so that the vitrification cryopreservation tube is favorable for placing the vitrification cryopreservation tube in the cryopreservation box.
The utility model discloses the beneficial effect who creates is:
1. the utility model has simple structure and convenient use, adopts a spiral structure to greatly increase the contact area, can vitrify and freeze immune cells in a large scale at one time, and improves the efficiency;
2. set up the support metal strip in the both sides of spiral pipe, can play the support fixed action, prevent the spiral rubber tube intertwine, make the spiral pipe can be smooth dip in the liquid nitrogen and freeze, the metal strip still possesses heat-conduction effect, can make the faster propagation of heat, improves and freezes and deposits efficiency.
Drawings
Fig. 1 is a schematic structural diagram of embodiment 1 of the present invention.
Fig. 2 is a schematic structural diagram of embodiment 2 of the present invention.
In the figure, 1-metal strip, 2-spiral calandria, 3-two-dimensional code label, 4-inlet and 5-outlet.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
As shown in figure 1, the utility model provides a pipe is deposited to spiral extensive immune cell vitrification freezing, including metal strip 1, spiral pipe 2 and two-dimensional code label 3, spiral pipe 2 is for around the circuitous dark low temperature resistant capillary of center pin multiple spiral, spiral freezing promptly and deposit the pipe, and the pipe diameter is 2mm, and the diameter of spiral is 30 mm.
The spiral cryopreservation pipe is provided with an inlet 4 and an outlet 5, and the inlet 4 and the outlet 5 are arranged at different ends of the spiral pipe 2. After the immune cells are injected, the inlet 4 and the outlet 5 of the spiral tube 2 are heat-sealed.
The metal strip 1 is installed to the both sides of spiral pipe 2, and the metal strip 1 passes through epoxy glue and bonds with spiral pipe 2. Two-dimensional code label 3 sets up at the entry end for information such as sign identity. Two-dimensional code label 3 is fixed on metal strip 1.
The spiral tube 2 is made of polypropylene materials, can resist the ultralow temperature of minus 196 ℃, is light in weight and can be rapidly cooled.
Example 2
As shown in figure 2, the utility model provides a pipe is deposited to spiral extensive immune cell vitrification freezing, including metal strip 1, spiral pipe 2 and two-dimensional code label 3, spiral pipe 2 is for around the circuitous dark low temperature resistant capillary of center pin multiple spiral, spiral freezing promptly deposits the pipe, and the pipe diameter is 2 mm.
The diameter of the vitrification cryopreservation tube is gradually reduced from top to bottom, and the lower end of the vitrification cryopreservation tube is smaller than the diameter of the upper end of the vitrification cryopreservation tube, so that the vitrification cryopreservation tube is favorable for placing the cryopreservation tube in the cryopreservation box. The spiral diameter of the spiral pipe is gradually reduced from top to bottom, and the change range is 27-30 mm.
The spiral cryopreservation pipe is provided with an inlet 4 and an outlet 5, and the inlet 4 and the outlet 5 are arranged at different ends of the spiral pipe 2. After the immune cells are injected, the inlet 4 and the outlet 5 of the spiral tube 2 are heat-sealed.
The metal strip 1 is installed to the both sides of spiral pipe 2, and the metal strip 1 passes through epoxy glue and bonds with spiral pipe 2. Two-dimensional code label 3 sets up at the entry end for information such as sign identity. Two-dimensional code label 3 is fixed on metal strip 1.
The spiral tube 2 is made of polypropylene materials, can resist the ultralow temperature of minus 196 ℃, is light in weight and can be rapidly cooled.
The above description is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are all covered by the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. The utility model provides a spiral large-scale immune cell vitrification freezes deposits pipe, a serial communication port, vitrification freezes deposits the pipe and includes spiral pipe, metal strip and two-dimensional code label, the spiral pipe is resistant dark low temperature capillary, and it includes import and export, import and export set up respectively the both ends of spiral pipe, the metal strip is installed the both sides of spiral pipe, the two-dimensional code label sets up the entry end of spiral pipe, vitrification freezes the diameter of depositing the pipe and from last to degressing down, the lower extreme is less than the diameter of upper end.
2. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the diameter of the spiral tube is 1mm to 5 mm.
3. The spiral large-scale immune cell vitrification cryopreservation tube of claim 2 wherein the diameter of the spiral tube is 2 mm.
4. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the diameter of the spiral tube spiral is 10mm to 50 mm.
5. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the inlet and outlet are heat sealed.
6. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the spiral tube is made of polypropylene material.
7. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the metal strip is bonded to the spiral tube by glue.
8. The spiral large-scale immune cell vitrification cryopreservation tube of claim 1 wherein the two-dimensional code label is fixed on the metal strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921500165.XU CN210869621U (en) | 2019-09-10 | 2019-09-10 | Spiral large-scale immune cell vitrification cryopreservation tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921500165.XU CN210869621U (en) | 2019-09-10 | 2019-09-10 | Spiral large-scale immune cell vitrification cryopreservation tube |
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| Publication Number | Publication Date |
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| CN210869621U true CN210869621U (en) | 2020-06-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921500165.XU Active CN210869621U (en) | 2019-09-10 | 2019-09-10 | Spiral large-scale immune cell vitrification cryopreservation tube |
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| CN (1) | CN210869621U (en) |
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- 2019-09-10 CN CN201921500165.XU patent/CN210869621U/en active Active
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