CN209784193U - Equipment capable of measuring Raman spectrum of substance under strong fluorescence background - Google Patents

Abstract

The utility model relates to a technical field of the equipment of the raman spectroscopy of measurement material discloses an equipment that can measure the raman spectroscopy of material under strong fluorescence background, include: laser for simultaneously emitting two excitation lights F with similar but different wavelengths₁And excitation light F₂(ii) a A laser driver for applying two excitation lights F emitted by the laser₁and excitation light F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂Is constantly maintained over timeChange and exciting light F₁intensity of and excitation light F₂The sum of the intensities of (a) and (b) does not vary with time; an optical conduction device; an optical collection device; a spectrometer; a signal processing device for performing a phase-locking operation on the Raman spectrum by using hardware or an algorithm; the utility model provides a can get rid of fluorescence to the interference of raman spectrum at the equipment of the raman spectrum of strong fluorescence under the background of fluorescence, accomplish extraction and the measurement to raman spectrum.

Description

Equipment capable of measuring Raman spectrum of substance under strong fluorescence background

Technical Field

The utility model belongs to the technical field of the equipment of the raman spectrum of measurement material and specifically relates to an equipment that can measure the raman spectrum of material under strong fluorescence background.

background

Raman spectroscopy is the scattering of a substance, when illuminated with light of a specific wavelength, out of a spectrum different from the incident light, and many molecules have their own unique raman spectra. The Raman spectrometer has obvious advantages in the aspect of chemical and biological molecule resolution by measuring the characteristic spectrum of molecules. By exciting a molecule to be measured with a laser having a fixed wavelength, measuring the spectral distribution of light emitted from the excited molecule, and by comparison, the kind, composition, etc. of the molecule to be measured can be revealed, and thus the method is widely used.

However, because materials such as a carrier of the molecule to be detected and the like have strong fluorescence, sometimes the fluorescence can cover the Raman spectrum of the molecule to be detected; how to complete the measurement of the raman spectrum under such a condition and how to expand the application range of the raman spectrum have been hot spots of the research of the spectroscopic technology.

In the prior art, a device capable of measuring raman spectra of substances in a strong fluorescent background is lacking.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a can be under strong fluorescence background measuring material's raman spectroscopy's equipment, aim at solving and lack the problem that can effectively get rid of the raman spectroscopy's of measuring material that fluorescence disturbed equipment among the prior art.

The utility model discloses a realize like this, a can measure equipment of raman spectrum of material under strong fluorescence background, include:

Laser for simultaneously emitting two excitation lights F with similar but different wavelengths₁And excitation light F₂；

A laser driver for applying two excitation lights F emitted by the laser₁And excitation light F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂with the intensity of the exciting light F varying with time₁Intensity of and excitation light F₂The sum of the intensities of (a) and (b) does not vary with time;

optical conduction means for passing said excitation light F along a first optical path₁And excitation light F₂Directing to the sample;

An optical collection device for collecting optical signals from the sample along a second optical path;

A spectrometer for splitting the optical signal collected by the optical collection device to generate a raman spectrum of the sample under inspection;

And the signal processing device is used for performing phase locking operation on the Raman spectrum by using hardware or an algorithm, removing the interference of fluorescence on the Raman spectrum and finishing the extraction and measurement of the Raman spectrum.

Further, the laser driver includes:

A first drive circuit for applying the excitation light F₁Modulating at a specific frequency;

A second drive circuit for applying the excitation light F₂Modulated at a particular frequency.

preferably, the first drive circuit modulates F as follows₁：

I₁＝I*cos(omega*t)；

The second drive circuit modulates F as follows₂：

I₂＝I*[1-cos(omega*t)]；

Wherein, I₁As excitation light F₁I is the excitation light F emitted by the laser₁Is also the excitation light F emitted by the laser₂T is the driving time, I₂As excitation light F₂Real-time intensity of the light.

Preferably, the first drive circuit modulates F as follows₁:

I₁＝I*sin(omega*t)；

The second drive circuit modulates F as follows₂：

I₂＝I*[1-sin(omega*t)]；

Wherein, I₁As excitation light F₁I is the excitation light F emitted by the laser₁is also the excitation light F emitted by the laser₂T is the driving time, I₂As excitation light F₂Real-time intensity of the light.

Compared with the prior art, the utility model provides a pair of can measure the equipment of raman spectrum of material under strong fluorescence background, through laser 1 simultaneous emission two close nevertheless inequality excitation light F of wavelength₁And excitation light F₂(ii) a Two excitation lights F emitted to the laser 1 by the laser driver 2₁And excitation light F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂With the intensity of the exciting light F varying with time₁Intensity of and excitation light F₂The sum of the intensities of (a) and (b) does not vary with time; the optical conduction device 3 transfers the excitation light F along a first light path₁And excitation light F₂Directing to the sample; the optical collection device 4 then collects the optical signal from the sample along a second optical path; the spectrometer 5 splits the optical signal collected by the optical collection device 4 to generate a raman spectrum of the sample under examination; and finally, the signal processing device 6 performs phase locking operation on the Raman spectrum by using hardware or an algorithm, removes the interference of fluorescence on the Raman spectrum, and finishes the extraction and measurement of the Raman spectrum.

drawings

Fig. 1 is a schematic diagram of an internal structure of an apparatus capable of measuring raman spectrum of a substance in a strong fluorescence background according to an embodiment of the present invention;

FIG. 2 shows the excitation light F₁Light intensity of (1), excitation light F₂Light intensity of (2) and excitation light F₁with excitation light F₂Respectively, as a function of time.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The same or similar reference numerals in the drawings of the present embodiment correspond to the same or similar components; in the description of the present invention, it should be understood that if there are the terms "upper", "lower", "left", "right", etc. indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of the description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore the terms describing the positional relationship in the drawings are only for illustrative purposes and are not to be construed as limitations of the present patent, and those skilled in the art can understand the specific meanings of the terms according to specific situations.

The following describes the implementation of the present invention in detail with reference to specific embodiments.

Referring to fig. 1-2, preferred embodiments of the present invention are provided.

An apparatus capable of measuring raman spectra of a substance in a strongly fluorescent background, comprising:

Laser 1 for simultaneously emitting two excitation lights F of similar but different wavelengths₁And excitation light F₂；

A laser driver 2 for applying two excitation lights F emitted from the laser 1₁and excitinglight F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂With the intensity of the exciting light F varying with time₁Intensity of and excitation light F₂The sum of the intensities of (a) and (b) does not vary with time;

an optical conduction device 3 for transmitting the excitation light F along a first light path₁And excitation light F₂Directing to the sample;

An optical collection device 4 for collecting optical signals from the sample along a second optical path;

A spectrometer 5 for splitting the optical signal collected by the optical collection device 4 to generate a raman spectrum of the sample under examination;

And the signal processing device 6 is used for performing phase locking operation on the Raman spectrum by using hardware or an algorithm, removing the interference of fluorescence on the Raman spectrum and finishing the extraction and measurement of the Raman spectrum.

According to the method for eliminating the fluorescence interference to measure the Raman spectrum of the substance, the influence of fluorescence is eliminated, and the extraction and measurement of the Raman spectrum are completed; the device has simple structure and accurate measurement result.

further, the laser driver 2 includes:

A first drive circuit for exciting light F₁Modulating at a specific frequency;

A second drive circuit for exciting light F₂modulated at a particular frequency.

In this embodiment, the first drive circuit modulates F as follows₁：

I₁＝I*cos(omega*t)；

the second drive circuit modulates F as follows₂：

I₂＝I*[1-cos(omega*t)]；

Wherein, I₁As excitation light F₁I is the excitation light F emitted by the laser 1₁Is also the excitation light F emitted by the laser 1₂T is the driving time, I₂As light of excitationF₂Real-time intensity of the light.

Alternatively, as another embodiment, the first drive circuit modulates F as follows₁:

I₁＝I*sin(omega*t)；

the second drive circuit modulates F as follows₂：

I₂＝I*[1-sin(omega*t)]；

Wherein, I₁as excitation light F₁I is the excitation light F emitted by the laser 1₁Is also the excitation light F emitted by the laser 1₂T is the driving time, I₂As excitation light F₂Real-time intensity of the light.

With the advance of laser technology, lasers 1 capable of emitting two wavelengths close to each other simultaneously have become available on the market, and the present invention uses a laser 1 capable of emitting two slightly different wavelengths simultaneously, i.e. a wavelength Iambda1 and a wavelength Iambda2, and adjusts the intensity of the laser light of these two wavelengths at a specific frequency, e.g. the intensity of the excitation light of Iambda1 is I₁Intensity of excitation light of wavelength Iambda2 is I₂Is driven by a first drive circuit to enable I₁and driving the first and second driving circuits to obtain I (omega) cos (omega) t₂＝I*[1-cos(omega*t)]Alternatively, I may be driven by the first driving circuit₁driving the first and second transistors by a first driving circuit₂＝I*[1-sin(omega*t)]Such that the total laser intensity I_t＝I₁+I₂The total laser intensity does not change with time, and the fluorescence response intensity of a substance is not sensitive to the excitation wavelength, so that the fluorescence intensity is unchanged under the irradiation of two lasers with similar wavelengths but unchanged total intensity.

However, in the case of the raman spectrum of a molecule, the difference in the number of wavelengths is wn, and is independent of the wavelength of excitation light. For excitation light of wavelength Iambda1, Raman scattered light with a wavelength difference wn at wavelength r₁Whereas for excitation light of wavelength iambda2, the raman scattered light of the wavenumber difference wn is at the wavelength r₂To (3). So at the wavelength r₁The raman scattering intensity at (a) will be modulated at omega frequency; similarly, at the wavelength r2, the raman scattering intensity is modulated by omega frequency, which appears on a constant fluorescence background, and the raman signal modulated by omega frequency can be subjected to phase locking operation on the spectrum by using hardware or an algorithm to extract a weak signal under a strong constant background, so that the extraction and measurement of the raman spectrum can be completed.

The device for measuring the Raman spectrum of a substance under the background of strong fluorescence provided by the above uses the laser 1 to simultaneously emit two excitation lights F with similar but different wavelengths₁And excitation light F₂(ii) a Two excitation lights F emitted to the laser 1 by the laser driver 2₁And excitation light F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂With the intensity of the exciting light F varying with time₁Intensity of and excitation light F₂The sum of the intensities of (a) and (b) does not vary with time; the optical conduction device 3 transfers the excitation light F along a first light path₁And excitation light F₂Directing to the sample; the optical collection device 4 then collects the optical signal from the sample along a second optical path; the spectrometer 5 splits the optical signal collected by the optical collection device 4 to generate a raman spectrum of the sample under examination; and finally, the signal processing device 6 performs phase locking operation on the Raman spectrum by using hardware or an algorithm, removes the interference of fluorescence on the Raman spectrum, and finishes the extraction and measurement of the Raman spectrum.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (2)

1. An apparatus capable of measuring raman spectra of a substance in a strongly fluorescent background, comprising:

Laser for simultaneously emitting two excitation lights F with similar but different wavelengths₁And excitation light F₂；

A laser driver for applying two excitation lights F emitted by the laser₁And excitation light F₂Are modulated at different specific frequencies, respectively, so that the excitation light F₁Intensity of and excitation light F₂With the intensity of the exciting light F varying with time₁Intensity of and excitation light F₂the sum of the intensities of (a) and (b) does not vary with time;

Optical conduction means for passing said excitation light F along a first optical path₁And excitation light F₂directing to the sample;

An optical collection device for collecting optical signals from the sample along a second optical path;

A spectrometer for splitting the optical signal collected by the optical collection device to generate a raman spectrum of the sample under inspection;

and the signal processing device is used for performing phase locking operation on the Raman spectrum by using hardware or an algorithm, removing the interference of fluorescence on the Raman spectrum and finishing the extraction and measurement of the Raman spectrum.

2. The apparatus of claim 1, wherein the laser driver comprises:

A first drive circuit for applying the excitation light F₁Modulating at a specific frequency;

A second drive circuit for applying the excitation light F₂Modulated at a particular frequency.