Summary of the invention
The utility model provides the multichannel optical fiber SPR sensorgram device with reference spectra, on an optical fiber probe
It realizes the distributed SPR sensorgram of multichannel, can be applicable to field of biomedicine at most while carrying out 12 kinds of spies of biofluid
Opposite sex detection, greatly improves work efficiency, and available real-time light source reference spectrum improves reality to compare reference
The accuracy tested, the channel for solving traditional distributed sensor is less, compares and analyzes there is no real-time light source light spectrum
Problem.
To achieve the above object, the utility model provides the multichannel optical fiber SPR sensorgram device for having reference spectra, the band
Have the multichannel optical fiber SPR sensorgram device of reference spectra by super continuum source, light source tail optical fiber, fiber optic splitter, single mode optical fiber,
Coupled lens group, seven core fibres, reception optical fiber and spectrometer composition;
Super continuum source is the super continuum source of spectral width 450nm -1100nm, for generating excitation SPR phenomenon
Exciting light;Light source tail optical fiber is used for transmission the light of super continuum source;Fiber optic splitter is used for the light of super continuum source point
For seven beams;Single mode optical fiber is used for the output light of reception optical fiber beam splitter, and coupled lens group is used for the output optocoupler of single mode optical fiber
It closes into seven core fibres;Seven core fibres are for generating simultaneously transmission sensing spectrum and transmission super continuum source reference spectra, seven cores
Optical fiber side is provided with SPR sensorgram area I, SPR sensorgram area II, SPR sensorgram area III, SPR sensorgram area IV, SPR sensorgram area V and SPR
Sensing unit VI, is provided with sensing groove structure in SPR sensorgram area I, the composition of SPR sensorgram area I include fibre core, nanometer metallic film and
Environmental liquids, fibre core are the bottom of sensing groove, and nanometer metallic film is covered on fibre core surface, and it is recessed that biofluid fills up sensing
Slot, SPR sensorgram area II, SPR sensorgram area III, SPR sensorgram area IV, SPR sensorgram area V, SPR sensorgram area VI and SPR sensorgram area I
Structure and composition is all the same;The light exit side of seven core fibres be provided with SPR sensorgram area VII, SPR sensorgram area VIII, SPR sensorgram area Ⅸ,
SPR sensorgram area Ⅹ, SPR sensorgram area Ⅺ, SPR sensorgram area Ⅻ and middle fiber core, SPR sensorgram area VII, SPR sensorgram area VIII, SPR sensorgram
Area Ⅸ, SPR sensorgram area Ⅹ, SPR sensorgram area Ⅺ and SPR sensorgram area Ⅻ be cone angle structure, SPR sensorgram area VII, SPR sensorgram area VIII,
SPR sensorgram area Ⅸ, SPR sensorgram area Ⅹ, SPR sensorgram area Ⅺ and SPR sensorgram area Ⅻ are identical as the composition in SPR sensorgram area I;Receive light
Fibre is for receiving simultaneously transmission sensing spectrum;Spectrometer spectral width 450nm -1100nm, spectrometer is for receiving and saving sensing
Spectrum;
The light exit side of super continuum source is connect with the receiving end of light source tail optical fiber, the light exit side and optical fiber of light source tail optical fiber
The optical receiving end of beam splitter connects, single mode optical fiber be provided with it is multiple, the light exit side of fiber optic splitter respectively with every single-mode optics
Fine optical receiving end connection, incident light corresponding with each fibre core of seven core fibres, coupling are saturating respectively for the fibre core of each single mode optical fiber
Microscope group is placed between the light exit side of single mode optical fiber and the optical receiving end of seven core fibres, and reception optical fiber is provided with more, more piece-root graftings
Receive optical fiber fibre core receive respectively seven core fibres peripheral fibre core output light, the fibre core of a root receiving fiber and seven core fibres
The welding of middle fiber core face;Spectrometer is provided with multiple, and light of the input terminal of multiple spectrometers respectively with more root receiving fibers goes out
Penetrate end connection.
As advanced optimizing for the utility model, the utility model has the multichannel optical fiber SPR sensorgram of reference spectra
Device, the light source tail optical fiber are single mode optical fiber or less fundamental mode optical fibre.
As advanced optimizing for the utility model, the utility model has the multichannel optical fiber SPR sensorgram of reference spectra
Device, the nanometer metallic film with a thickness of 50nm.
As advanced optimizing for the utility model, the utility model has the multichannel optical fiber SPR sensorgram of reference spectra
Device, the surface of the nanometer metallic film are also covered with antigen or complementary DNA or other specific binding bodies.
As advanced optimizing for the utility model, the utility model has the multichannel optical fiber SPR sensorgram of reference spectra
Device, the reception optical fiber are gradual change multimode fibre or step multimode fibre.
This practical new multichannel optical fiber SPR sensorgram device with reference spectra has the beneficial effect that
1. the utility model provides the multichannel optical fiber SPR sensorgram device with reference spectra, in an optical fiber probe
The distributed SPR sensorgram of upper realization multichannel can be applicable to field of biomedicine at most while carry out 12 kinds of biofluid
Specific detection greatly improves work efficiency, and available real-time light source reference spectrum is improved to compare reference
The accuracy of experiment, the channel for solving traditional distributed sensor is less, compares and analyzes there is no real-time light source light spectrum
The problem of.
2. the utility model has made the integrated distributed spr sensor in six channels, six tunnels distribution can be carried out simultaneously
Sensing, improves sensing efficiency.
3. the utility model is applied in field of biomedicine, the six of biofluid can be at most realized simultaneously by the sensor
Species specificity analysis detection, work efficiency is high.
4. independent, the glitch-free sensing spectrum that the utility model obtains the analysis of six tunnel specificity.
5. the reference spectra that the utility model can simultaneously obtain real-time light source is used as comparative analysis, the standard of experiment is enhanced
True property.
6. the utility model making material is simply easily sought, low in cost.
7. the utility model can repeatedly use, save the cost is highly reliable.
Specific embodiment
Illustrate present embodiment below with reference to Fig. 1,2,3, the utility model provides the multichannel light with reference spectra
Fine SPR sensorgram device realizes the distributed SPR sensorgram of multichannel on an optical fiber probe, can be applicable to field of biomedicine
The 12 species specificity detection at most carrying out biofluid simultaneously, greatly improves work efficiency, and available real-time light
Source reference spectra is referred to compare, and improves the accuracy of experiment, and the channel for solving traditional distributed sensor is less, and
There is no the problem of real-time light source light spectrum compares and analyzes.
To achieve the above object, the utility model provides the multichannel optical fiber SPR sensorgram device for having reference spectra, this is new
Multichannel optical fiber SPR sensorgram device of the type with reference spectra is by super continuum source 1, light source tail optical fiber 2, fiber optic splitter 3, list
Mode fiber 4, coupled lens group 5, seven core fibres 6, reception optical fiber 7 and spectrometer 8 form;
Super continuum source 1 is the super continuum source of spectral width 450nm -1100nm, existing for generating excitation SPR
The exciting light of elephant, the resonant wavelength range of traditional fiber spr sensor are 450nm -1100nm, the spectrum of super continuum source 1
Width need to include 450nm -1100nm, at least 450nm -1100nm;Light source tail optical fiber 2 is used for transmission super continuum source 1
Light;Fiber optic splitter 3 is used to the light of super continuum source 1 being divided into seven beams;Single mode optical fiber 4 is for reception optical fiber beam splitter 3
Output light, coupled lens group 5 is for the output light of single mode optical fiber 4 to be coupled into seven core fibres 6;Seven core fibres 6 are for generating
And transmission sensing spectrum and transmission light source reference spectra, seven core fibres, 6 side are provided with I 6-1 of SPR sensorgram area, SPR sensorgram area II
6-2, III 6-3 of SPR sensorgram area, IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area and SPR sensorgram area VI 6-6, I 6- of SPR sensorgram area
Sensing groove is provided on 1, I 6-1 of SPR sensorgram area composition includes fibre core 6-1-1, nanometer metallic film 6-1-2 and environmental liquids
6-1-3, fibre core 6-1-1 are the bottom of sensing groove, and nanometer metallic film 6-1-2 is covered on the surface fibre core 6-1-1, biofluid
6-1-3 fills up sensing groove, II 6-2 of SPR sensorgram area, III 6-3 of SPR sensorgram area, IV 6-4 of SPR sensorgram area, V 6- of SPR sensorgram area
5, the structure and composition of I 6-1 of VI 6-6 of SPR sensorgram area and SPR sensorgram area is all the same;The light exit side of seven core fibres 6 is provided with
VII 6-7 of SPR sensorgram area, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area,
SPR sensorgram area Ⅻ 6-12 and middle fiber core 6-13, VII 6-7 of SPR sensorgram area, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area,
Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area and Ⅻ 6-12 of SPR sensorgram area are cone angle structure, SPR sensorgram area VII 6-7, SPR
VIII 6-8 of sensing unit, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area and Ⅻ 6-12 of SPR sensorgram area with
The composition of I 6-1 of SPR sensorgram area is identical;Reception optical fiber is for receiving simultaneously transmission sensing spectrum;8 spectral width 450nm of spectrometer-
1100nm, spectrometer 8 is for receiving and saving sensing spectrum;
The light exit side of super continuum source 1 is connect with the receiving end of light source tail optical fiber 2, the light exit side of light source tail optical fiber 2 with
The optical receiving end of fiber optic splitter 3 connects, single mode optical fiber 4 be provided with it is multiple, the light exit side of fiber optic splitter 3 respectively with every
The optical receiving end of single mode optical fiber 4 connects, and the fibre core of each single mode optical fiber 4 is corresponding with each fibre core of seven core fibres 6 incident respectively
Light, coupled lens group 5 are placed between the light exit side of single mode optical fiber 4 and the optical receiving end of seven core fibres 6, and reception optical fiber 7 is arranged
There are more, the fibre core of more root receiving fibers 7 receives the output light of the peripheral fibre core of seven core fibres 6 respectively, a root receiving fiber 7
The welding of the middle fiber core 6-13 face of fibre core and seven core fibres 6;Spectrometer 8 is provided with multiple, the input terminal point of multiple spectrometers 8
It is not connect with the light exit side of more root receiving fibers 7;Manufacturing process: the single mode optical fiber 4 of seven 30cm or so, single mode optical fiber 4 are taken
Coat is stripped using optical fiber pincers at the 1cm-2cm of both ends, the mixed liquor of alcohol and ether is dipped with non-woven fabrics, is wiped repeatedly covering
Cleaned, both ends of the surface are cut by optical fiber cutter it is smooth, by more single mode optical fibers 4 respectively with the output of fiber optic splitter
End connection is taken seven core fibres 6 of one section of 40cm-50cm, is clamped at the 1cm-2cm of optical fiber both ends using optical fiber and strip coat, with nothing
Woven fabric dips the mixed liquor of alcohol and ether, is wiped repeatedly covering and is cleaned, and cuts both ends of the surface by optical fiber cutter and puts down
Whole, six fibre core wheat flours for choosing seven core fibres, 6 side make I 6-1 of SPR sensorgram area, II 6- of SPR sensorgram area of excitation SPR phenomenon
2, III 6-3 of SPR sensorgram area, VI 6-6 of IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area and SPR sensorgram area, utilize optical fiber wire stripper
Coat is stripped, the mixed liquor of alcohol and ether is dipped with non-woven fabrics, covering is wiped repeatedly and is cleaned, then, passes through optical fiber
The sensing unit part side throwing of selection is removed covering by side throwing machine, leaks out fibre core, the mixing of alcohol and ether is dipped with non-woven fabrics
Liquid is wiped repeatedly the cleaning conical surface, and the sensing unit surface for leaking out fibre core is being plated nano metal respectively by small ion sputtering
Film 6-1-2, nanometer metallic film 6-1-2 are the golden film of 50nm thickness, take out after plated film, the light exit side of seven core fibres 6 is set
In six fibre cores of the periphery of seven core fibres 6 are worn into cone angle type SPR sensorgram structure respectively on optical fiber cone grinding machine, cone angle range is
8 ° -20 °, the sensing unit surface for leaking out fibre core is plated by the light exit side of seven core fibres 6 respectively by small ion sputtering and is received
Rice metallic film 6-1-2, nanometer metallic film 6-1-2 are the golden film of 50nm thickness, are taken out after plated film, SPR sensorgram area VII 6-7, SPR
VIII 6-8 of sensing unit, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area, Ⅻ 6-12 of SPR sensorgram area and
Middle fiber core 6-13, the ends cutting of the optical receiving end of seven root receiving fibers 7 is smooth, receive each seven by reception optical fiber 7
Coupled lens group 5 is placed in the light exit side of single mode optical fiber 4 and the optical receiving end of seven core fibres 6 by the emergent light of 6 fibre core of core fibre
Between, so that the fibre core of seven core fibres 6 receives the emergent light of single mode optical fiber 4, the light exit side of seven root receiving fibers 7 connects respectively
To different spectrometers 8;The course of work: the switch of super continuum source 1 is opened, and the switch of spectrometer 8 is opened, super continuous spectrums light
The light in source 1 is transmitted by light source tail optical fiber 2, and the light of light source tail optical fiber 2 is received by fiber optic splitter 3, and light is divided into seven beams by seven
Root single mode optical fiber 4 exports, and output light is coupled into seven of seven core fibres 6 by coupled lens group 5 by the output light of single mode optical fiber 4
In fibre core, be uniformly distributed outside the fibre cores of seven core fibres 6 six, center there is a middle fiber core 6-13, middle fiber core 6-13 to use
I 6-1 of SPR sensorgram area, II 6- of SPR sensorgram area have been made in six fibre cores of the reference spectra of transmission light source, seven core fibres, 6 outside
2, III 6-3 of SPR sensorgram area, VI 6-6 of IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area and SPR sensorgram area, in seven core fibres, 6 light
Exit end has VII 6-7 of SPR sensorgram area, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, SPR sensorgram
Ⅺ 6-11 of area, SPR sensorgram area Ⅻ 6-12 and middle fiber core 6-13;Light in seven core fibres 6 passes through I 6-1 of SPR sensorgram area, environment
Liquid 6-1-3, II 6-2 of SPR sensorgram area, III 6-3 of SPR sensorgram area, IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area, SPR sensorgram
VI 6-6 of area, VII 6-7 of SPR sensorgram area, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, SPR sensorgram
The free electron of the nanoporous metal membrane of Ⅻ 6-12 of Ⅺ 6-11 of area and SPR sensorgram area, light and sensing unit resonates, and resonance simultaneously can
It is sensed with the refractive index to environmental liquids;It applies in field of biomedicine, can be covered on nanoporous metal membrane different
It specifically binds body (such as antigen or complementary DNA), environmental liquids is changed to biofluid to be measured, when exciting SPR phenomenon, if
If biofluid to be measured react with specific binding body generation, by taking antigen as an example, can detecte several fruits to be measured it is to be measured in whether
Lead to the resonance wave of SPR sensorgram spectrum because specific binding can change the refractive index of biofluid containing corresponding antibody
Paddy, resonance depth change, and reception optical fiber 7 receives in sensing spectrum incoming light spectrometer 8, observe on corresponding spectrometer 8
Sense spectrum, sense spectrum resonance trough, resonance depth can show the characteristic specificity, observe and record preservation sense light
Spectrum, experiment, which finishes, closes the switch of super continuum source 1 and spectrometer 8;The utility model realizes that multichannel is distributed
Spr sensor is such as applied in field of biomedicine, at most can once be carried out the detection of 12 species specificity, can also be obtained super company
The real time spectrum of continuous spectrum light source 1 is analyzed as bench-marking, and work efficiency is high, improves accuracy.
The utility model has the multichannel optical fiber SPR sensorgram device of reference spectra, and the light source tail optical fiber 2 is single-mode optics
Fine or less fundamental mode optical fibre.
The utility model has the multichannel optical fiber SPR sensorgram device of reference spectra, the nanometer metallic film 6-1-2
With a thickness of 50nm.
The utility model has the multichannel optical fiber SPR sensorgram device of reference spectra, the nanometer metallic film 6-1-2
Surface be also covered with antigen or complementary DNA or other specific binding bodies.
The utility model has the multichannel optical fiber SPR sensorgram device of reference spectra, and the reception optical fiber 7 is that gradual change is more
Mode fiber or step multimode fibre.
The working principle of the utility model is:
The switch of super continuum source 1 is opened, and the switch of spectrometer 8 is opened, and the light of super continuum source 1 passes through light source tail
Fibre 2 transmits, and the light of light source tail optical fiber 2 is received by fiber optic splitter 3, and light is divided into seven beams and is exported by seven single mode optical fibers 4, single
Output light is coupled into seven fibre cores of seven core fibres 6 by the output light of mode fiber 4 by coupled lens group 5, seven core fibres 6
Be uniformly distributed outside fibre core six, center there is a middle fiber core 6-13, middle fiber core 6-13 to be used for transmission the reference light of light source
Spectrum, six fibre cores in the outside of seven core fibre 6 made I 6-1 of SPR sensorgram area, II 6-2 of SPR sensorgram area, III 6-3 of SPR sensorgram area,
VI 6-6 of IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area and SPR sensorgram area has SPR sensorgram area in seven core fibres, 6 light exit side
VII 6-7, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area, SPR sensorgram area
Ⅻ 6-12 and middle fiber core 6-13;Light in seven core fibres 6 passes through I 6-1 of SPR sensorgram area, environmental liquids 6-1-3, SPR sensorgram area
II 6-2, III 6-3 of SPR sensorgram area, IV 6-4 of SPR sensorgram area, V 6-5 of SPR sensorgram area, VI 6-6 of SPR sensorgram area, SPR sensorgram area VII
6-7, VIII 6-8 of SPR sensorgram area, Ⅸ 6-9 of SPR sensorgram area, Ⅹ 6-10 of SPR sensorgram area, Ⅺ 6-11 of SPR sensorgram area and SPR sensorgram area
The free electron of the nanoporous metal membrane of Ⅻ 6-12, light and sensing unit resonates, and resonance simultaneously can be to the refraction of environmental liquids
Rate is sensed;It applies in field of biomedicine, different specific binding body (such as antigen can be covered on nanoporous metal membrane
Or complementary DNA etc.), environmental liquids are changed to biofluid to be measured, when exciting SPR phenomenon, if if biofluid to be measured and spy
Anisotropic combination generates reaction, by taking antigen as an example, can detecte number fruits to be measured it is to be measured in whether containing corresponding antibody because
Specific binding can change the refractive index of biofluid, and the resonance trough of SPR sensorgram spectrum, resonance depth is caused to change,
Reception optical fiber 7 receives in sensing spectrum incoming light spectrometer 8, and observation sensing spectrum, senses being total to for spectrum on corresponding spectrometer 8
Vibration wave paddy, resonance depth can show characteristic specificity, observe and record preservation sensing spectrum, and experiment is finished super continuous spectrums
The switch of light source 1 and spectrometer 8 is closed;The utility model realizes multichannel distribution spr sensor, such as applies in biology
Medical domain at most can once carry out the detection of 12 species specificity, the real time spectrum conduct of super continuum source 1 also can be obtained
Bench-marking analysis, work efficiency is high, improves accuracy.
Certainly, above description is not limitation of the utility model, and the utility model is also not limited to the example above, this skill
The variations, modifications, additions or substitutions that the those of ordinary skill in art field is made in the essential scope of the utility model, also belong to
In the protection scope of the utility model.