CN207330802U - A kind of integral type protein purification chromatographic apparatus - Google Patents
A kind of integral type protein purification chromatographic apparatus Download PDFInfo
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- CN207330802U CN207330802U CN201720235443.8U CN201720235443U CN207330802U CN 207330802 U CN207330802 U CN 207330802U CN 201720235443 U CN201720235443 U CN 201720235443U CN 207330802 U CN207330802 U CN 207330802U
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- peristaltic pump
- liquid crystal
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Abstract
The purpose of the application is to provide a kind of integral type protein purification chromatographic apparatus, it is characterised in that it includes control device and sequentially connected sampling pump, pressure sensor device, mixer, on-line degassing device, flowmeter, chromatographic column, detection module, solenoid valve, sample collection bottle and waste collection bottle；The control device is electrically connected and controls the sampling pump, pressure sensor device, mixer, on-line degassing device, flowmeter, chromatographic column, detection module and solenoid valve；Integral type protein purification chromatographic apparatus provided by the utility model, the degree of automation is high, and all operations can be completed on instrument panel, without additionally configuring computer, convenient operation, saves lab space, easy to operate, stability is good, purity is high, energy consumption is low, applied widely, market prospects are good.
Description
Technical field
The invention belongs to biological purification device, and in particular to a kind of chromatographic apparatus of protein purification.
Background technology
At present, biomedicine field development in China's is swift and violent, and Bio-pharmaceutical Industry has formd certain scale, is expected to become
Next pillar industry.
Protein is to form human body all cells, the important component of tissue, and all important parts of body are required for
There is the participation of protein.Pharmaceutical grade protein can be divided into polypeptide and genetically engineered drug, monoclonal antibody and genetic engineering antibody, again
Group vaccine etc., compared with conventional small-molecule chemical medicine, the active strong, high specificity of pharmaceutical grade protein, toxicity are low, biological
Definite functions, be conducive to the characteristics of clinical practice.Since its cost is low, success rate is high, safe and reliable, it has also become in medical product
Important component.
Chromatography is a kind of extremely effective method of protein isolate matter, can be with the protein list of isolated high-purity
Body.Current conventional use of protein purification chromatographic apparatus is generally split-type design, and the degree of automation is not high, it is necessary to additionally configure
Computer is controlled, occupancy lab space is larger, and operation is relatively complicated.
And the detector in protein purification chromatographic apparatus of the prior art can only generally detect ultraviolet absorptivity, it is impossible to same
When detect electrical conductivity, the key parameter such as pH value, need to additionally purchase conductivity meter and pH meter, and use it is also inconvenient, it is impossible to
Detection in real time.
Therefore, it is badly in need of a kind of good purification devices, above-mentioned the problems of the prior art can be solved.
The content of the invention
The main problem that the application solves is to provide a kind of unitary design, and the degree of automation is high, can be on instrument panel
Complete all operations, without extra configuration computer, convenient operation, save lab space, easy to operate, stability is good, purity is high,
The low protein purification chromatographic apparatus of energy consumption, with solve a kind of preparation method is complicated, use scope is limited, stability is not high,
Purity is low and the technical problem of high energy consumption.
In order to solve the above-mentioned technical problem, the invention discloses a kind of integral type protein purification chromatographic apparatus, its technical side
Case is as follows：
A kind of integral type protein purification chromatographic apparatus, it is characterised in that including control device and sequentially connected sampling pump,
Pressure sensor device, mixer, on-line degassing device, flowmeter, chromatographic column, detection module, sample collection bottle and waste liquid are received
Collect bottle；
The control device is electrically connected and controls the sampling pump, pressure sensor device, mixer, on-line degassing dress
Put, flowmeter, chromatographic column.
Preferably, the sampling device being arranged between the on-line degassing device and the flowmeter is further included.
Preferably, the sampling pump includes two peristaltic pumps, is connected by three-way pipe with the pressure sensor.
Preferably, the detection module includes UV detector, electric conductivity detector and the pH sensor being sequentially connected in series.
Preferably, the control device is wide including peristaltic pump processing module, liquid crystal driver module, liquid crystal touch screen, pulse
Spend modulation (PWM) drive circuit, temperature measurement circuit, auxiliary circuit and PWM speed-regulating fans；Wherein：
Contact control module, the operational order sent for receiving user by liquid crystal touch screen, at the peristaltic pump
Reason module is operated；
Peristaltic pump processing module, for receiving the operational order that is sent from contact control module, to the operational order into
Row processing, and handling result is included handling result on liquid crystal display by liquid crystal driver module；It is additionally operable to operation branch
Hold the peristaltic pump application software of pid algorithm；
Liquid crystal driver module, for receiving processing result information of the peristaltic pump processing module to operational order, and drives institute
State liquid crystal touch screen and show the result information；
Liquid crystal touch screen, for the operational order inputting interface as the contact control module, and for showing through compacted
Result information after dynamic pump processing module processing；
Pwm circuit, for receiving the instruction of the microprocessor, is adjusted the rotating speed of PWM speed-regulating fans；
Temperature measurement circuit, the microprocessor is sent to for monitoring the temperature in the peristaltic pump, and by temperature test result
Device with pass through pwm circuit adjust PWM speed-regulating fans rotating speed；
The auxiliary circuit, for tracking the temperature measurement circuit, if temperature is less than default lower limit or higher than on default
Limit value, by man-machine interface display alarm information and sends alarm sound.
Preferably, the control device further includes printer interface and USB interface.
Preferably, the peristaltic pump processing module includes：Microprocessor, driver, real time operating system and wriggling
Pump application layer software.
Integral type protein purification chromatographic apparatus provided by the invention, the degree of automation is high, and institute can be completed on instrument panel
There is operation, without extra configuration computer, convenient operation, saves lab space, easy to operate, stability is good, purity is high, energy consumption
Low, applied widely, market prospects are good.
Brief description of the drawings
Attached drawing described herein is used for providing a further understanding of the present invention, forms the part of the present invention, this hair
Bright schematic description and description is used to explain the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is the flow diagram of protein purification chromatography method provided by the present invention.
Wherein correspondence is：1st, the first peristaltic pump；2nd, the second peristaltic pump；3rd, pressure sensor device；4th, mixer；5th, exist
Line degasser；6th, sampling device；7th, flowmeter；8th, chromatographic column；9th, UV detector；10th, electric conductivity detector；11st, pH value is examined
Survey device；12nd, solenoid valve；13rd, waste collection bottle；14th, sample collection bottle.
Embodiment
Some vocabulary has such as been used to censure specific components among specification and claim.Those skilled in the art should
It is understood that hardware manufacturer may call same component with different nouns.This specification and claims are not with name
The difference of title is used as the mode for distinguishing component, but is used as the criterion of differentiation with the difference of component functionally.Specification
Subsequent descriptions for implement the application better embodiment, so it is described description be for the purpose of the rule for illustrating the application,
It is not limited to scope of the present application.The protection domain of the application is when subject to appended claims institute defender.
The application is described in further detail below in conjunction with attached drawing, but not as the restriction to the application.
Embodiment one：
As shown in the structure diagram of Fig. 1 integral type protein purification chromatographic apparatus provided by the present invention, a kind of integral type
Protein purification chromatographic apparatus, it is characterised in that including control device and sequentially connected sampling pump, pressure sensor device 3, mixing
Device 4, on-line degassing device 5, flowmeter 7, chromatographic column 8, detection module, sample collection bottle 14 and waste collection bottle 13；
The control device is electrically connected and controls the sampling pump, pressure sensor device 3, mixer 4, on-line degassing
Device 5, flowmeter 7, chromatographic column 8.
The integral type protein purification chromatographic apparatus, which further includes, is arranged at the on-line degassing device 5 and the flowmeter 7
Between sampling device 6.
The sampling pump includes two peristaltic pumps, and the first peristaltic pump 1 and the second peristaltic pump 2 pass through three-way pipe and the pressure
Sensor 3 connects.Using dual pump design, gradient elution can be carried out.
The detection module includes UV detector 9, electric conductivity detector 10 and the pH value detector 11 being sequentially connected in series.
The control device includes peristaltic pump processing module, liquid crystal driver module, liquid crystal touch screen, pulse width modulation
PWM drive circuit, temperature measurement circuit, auxiliary circuit and PWM speed-regulating fans；The peristaltic pump processing module includes：Microprocessor, drive
Dynamic program, real time operating system and peristaltic pump application layer software, wherein：
Contact control module, the operational order sent for receiving user by liquid crystal touch screen, at the peristaltic pump
Reason module is operated；
Peristaltic pump processing module, for receiving the operational order that is sent from contact control module, to the operational order into
Row processing, and handling result is included handling result on liquid crystal display by liquid crystal driver module；It is additionally operable to operation branch
Hold the peristaltic pump application software of pid algorithm；
Liquid crystal driver module, for receiving processing result information of the peristaltic pump processing module to operational order, and drives institute
State liquid crystal touch screen and show the result information；
Liquid crystal touch screen, for the operational order inputting interface as the contact control module, and for showing through compacted
Result information after dynamic pump processing module processing；
Pwm circuit, for receiving the instruction of the microprocessor, is adjusted the rotating speed of PWM speed-regulating fans；
Temperature measurement circuit, the microprocessor is sent to for monitoring the temperature in the peristaltic pump, and by temperature test result
Device with pass through pwm circuit adjust PWM speed-regulating fans rotating speed；
The auxiliary circuit, for tracking the temperature measurement circuit, if temperature is less than default lower limit or higher than on default
Limit value, by man-machine interface display alarm information and sends alarm sound.By newly-increased contact control module, liquid crystal driver module and
The hardware such as liquid crystal touch screen, coordinate corresponding peristaltic pump application software, reduce the difficulty of parameter setting when peristaltic pump uses；It is logical
The guide function of expert system is crossed, further by setting intelligence, the simplification of peristaltic pump, use and market beneficial to peristaltic pump
Promote.The peristaltic pump by the monitoring to environment temperature in peristaltic pump, according to temperature detection value, controlled using microprocessor
PWM drive circuit and PWM speed-regulating fans work, and peristaltic pump is always worked within the scope of safe temperature, and further
The rotating speed of PWM speed-regulating fans is adjusted using pid algorithm dynamic, had not only protected the safe handling of peristaltic pump, but also reduces energy consumption, together
When, also improve the service life and functional reliability of peristaltic pump.
The control device further includes printer interface and USB interface.Easy to the printing and transmission of data.
Integral type protein purification chromatographic apparatus provided by the invention, the degree of automation is high, and institute can be completed on instrument panel
There is operation, without extra configuration computer, convenient operation, saves lab space, easy to operate, stability is good, purity is high, energy consumption
Low, applied widely, market prospects are good.
Some preferred embodiments of the application have shown and described in described above, but as previously described, it should be understood that the application
Be not limited to form disclosed herein, be not to be taken as the exclusion to other embodiment, and available for various other combinations,
Modification and environment, and above-mentioned teaching or the technology or knowledge of association area can be passed through in application contemplated scope described herein
It is modified., then all should be in this Shen and changes and modifications made by those skilled in the art do not depart from spirit and scope
Please be in the protection domain of appended claims.
Claims (5)
1. a kind of integral type protein purification chromatographic apparatus, it is characterised in that including control device and sequentially connected sampling pump, pressure
Force sensor, mixer, on-line degassing device, flowmeter, chromatographic column, detection module, sample collection bottle and waste collection
Bottle；
The control device is electrically connected and controls the sampling pump, pressure sensor device, mixer, on-line degassing device,
Flowmeter, chromatographic column.
2. integral type protein purification chromatographic apparatus according to claim 1, it is characterised in that further include be arranged at it is described
Sampling device between line degasser and the flowmeter.
3. integral type protein purification chromatographic apparatus according to claim 2, it is characterised in that the sampling pump includes two
Peristaltic pump, is connected by three-way pipe with the pressure sensor.
4. integral type protein purification chromatographic apparatus according to claim 3, it is characterised in that the detection module include according to
UV detector, electric conductivity detector and the pH value detector of secondary series connection.
5. integral type protein purification chromatographic apparatus according to claim 4, it is characterised in that the control device includes compacted
Dynamic pump processing module, liquid crystal driver module, liquid crystal touch screen, pulse width modulation (PWM) drive circuit, temperature measurement circuit, auxiliary electricity
Road and PWM speed-regulating fans；
Contact control module, the operational order sent for receiving user by liquid crystal touch screen, mould is handled to the peristaltic pump
Block is operated；
Peristaltic pump processing module, for receiving the operational order sent from contact control module, at the operational order
Reason, and handling result is included handling result on liquid crystal display by liquid crystal driver module；It is additionally operable to operation support PID
The peristaltic pump application software of algorithm；The peristaltic pump processing module includes：Microprocessor, driver, real time operating system with
And peristaltic pump application layer software, wherein：
Liquid crystal driver module, for receiving processing result information of the peristaltic pump processing module to operational order, and drives the liquid
Brilliant touch-screen shows the result information；
Liquid crystal touch screen, for the operational order inputting interface as the contact control module, and for showing through peristaltic pump
Result information after processing module processing；
Pwm circuit, for receiving the instruction of the microprocessor, is adjusted the rotating speed of PWM speed-regulating fans；
Temperature measurement circuit, for monitoring the temperature in the peristaltic pump, and by temperature test result be sent to the microprocessor with
The rotating speed of PWM speed-regulating fans is adjusted by pwm circuit；
The auxiliary circuit, for tracking the temperature measurement circuit, if temperature less than default lower limit or higher than preset upper limit value,
By man-machine interface display alarm information and alarm sound is sent, the control device further includes printer interface and USB interface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720235443.8U CN207330802U (en) | 2017-03-10 | 2017-03-10 | A kind of integral type protein purification chromatographic apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720235443.8U CN207330802U (en) | 2017-03-10 | 2017-03-10 | A kind of integral type protein purification chromatographic apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749503A (en) * | 2017-03-10 | 2017-05-31 | 湖南铭沃生物科技有限公司 | A kind of integral type protein purification chromatographic apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106749503A (en) * | 2017-03-10 | 2017-05-31 | 湖南铭沃生物科技有限公司 | A kind of integral type protein purification chromatographic apparatus |
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