CN206945543U - Probe unit, electronic cassette preforming device for chemiluminescence detection - Google Patents
Probe unit, electronic cassette preforming device for chemiluminescence detection Download PDFInfo
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- CN206945543U CN206945543U CN201720692364.XU CN201720692364U CN206945543U CN 206945543 U CN206945543 U CN 206945543U CN 201720692364 U CN201720692364 U CN 201720692364U CN 206945543 U CN206945543 U CN 206945543U
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- probe unit
- flat board
- detection
- magazine
- lower box
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Abstract
The utility model provides a kind of probe unit, electronic cassette preforming device for chemiluminescence detection, the probe unit includes magazine, flat board probe unit is set in the magazine, the flat board probe unit horizontal is in magazine, the magazine includes upper cartridge body and lower box body, the upper cartridge body and lower box body matching, the flat board probe unit are located in upper cartridge body or lower box body；When the flat board probe unit is located in upper cartridge body, detection platform is additionally provided with the lower box body, the detection platform is flat on below flat board probe unit at 1 ~ 2mm, when the flat board probe unit is located in lower box body itself as detection platform.The electronic cassette preforming device includes the probe unit.The utility model conveniently can carry out qualitative and quantitative analysis to molecular engram film, especially chemiluminescence Western blotting film.
Description
Technical field
It the utility model is related to a kind of probe unit, electronic cassette preforming device for chemiluminescence detection.
Background technology
The process that large biological molecule is transferred on a kind of fixed matrix membrane from gel is referred to as engram technology, and oneself is widely used in
DNA, RNA, protein detection.Immunoblotting (Western Blot) is molecular biology, biochemistry and immune something lost
Pass a kind of experimental method commonly used in learning.Its general principle is the cell or life separated by specific antibody to gel electrophoresis
Thing tissue sample is coloured.By analyze coloring position and color depth obtain specified protein in the cell analyzed or
The information of expression in tissue.
ProBloot membrane chemiluminescence detection is one of protein analytical techniques means the most commonly used in Biochemistry Experiment,
Qualitative and quantitative analysis can be carried out to albumen.The detection method of current chemiluminescence blotting membrane has two kinds：Magazine tabletting (glue
Piece is photosensitive) and electronic CCD video generation.The principle of magazine tabletting is that blotting membrane luminous signal is carried out directly using photographic film
It is photosensitive, so as to obtain the chemiluminescence signal intensity on blotting membrane.Electronic CCD detection technique is then to use inductive electronics device
Part catches the luminous signal on blotting membrane, and optical signal is converted into electronic signal in CCD chip, so as to realize detection.More than
Two methods respectively have its advantage and disadvantage：Film cassette preforming device is simple and high sensitivity, but need to be fixed development by hand, in developer solution
Containing hypertoxic cyanide, maximum inferior position is can not to directly obtain digital measuring result (result is stored on film)；Electronic CCD
Technology can directly obtain digital measuring result, but sensitivity is not as good as magazine tabletting, and device is larger more complicated.So secretly
Although box tabletting ProBloot membrane detection technique has the usage history of more than 20 years, but do not substituted yet by electronic CCD technology, according to
It is so the mainstream technology in the field.
A variety of flat panel detectors based on semiconductor composite material can all convert optical signals to electric signal, in the pole of photoelectricity two
Storage electric charge is formed on the electric capacity of pipe or transistor itself, its amount of charge stored is directly proportional to incident ray intensity, in control electricity
In the presence of road, scanning reads the storage electric charge of pixel, the output digit signals after A/D is changed, sends computer generation pair to
Answer digitized video.Flat panel detector detection technique have the advantages that spatial resolution it is high, can normal-temperature operation, photosensitive area it is big, should
Technology is widely used to medical DR equipment and industrial flaw detection equipment (being X-ray detection), but has no that it is used for chemiluminescence
The device report of (i.e. visible ray) detection.
Utility model content
In view of the above the shortcomings that prior art, the purpose of this utility model is that providing one kind is used for chemiluminescence inspection
Probe unit, the electronic cassette preforming device of survey, for solving the problems of the prior art.
In order to achieve the above objects and other related objects, flat board probe unit, the flat board detection are set in the magazine
For unit horizontal in magazine, the magazine includes upper cartridge body and lower box body, the upper cartridge body and lower box body matching, described flat
Plate probe unit is located in upper cartridge body or lower box body；When the flat board probe unit is located in upper cartridge body, the lower box body
Detection platform is inside additionally provided with, the detection platform is flat on below flat board probe unit at 1~2mm；When the flat board detects list
Itself as detection platform when member is located in lower box body.
The upper cartridge body and lower box body matching refer to that upper cartridge body is covered when on lower box body, can be formed one it is closed
Space.
When flat board probe unit is located at upper cartridge body, the detection platform is used to place detected material, such as blotting membrane；Work as institute
When stating flat board detection unit itself as detection platform, its own can be used for placing and supporting detected material, while can be with
For detecting detected material.
The horizontal can be placement horizontal or with small skew degree, and most preferably scheme is horizontal positioned.
In the utility model, the flat panel detector then without cesium iodide coating, can directly detect chemiluminescence pair
Answer wavelength.
Preferably, the flat board probe unit is selected from non-crystalline silicon photo-transistor arrays detector, the pole of non-crystalline silicon photoelectricity two
Appointing in pipe detector array, low temperature polycrystalline silicon photo-transistor arrays detector or crystalline silicon photo-transistor arrays detector
Meaning is a kind of.
Non-crystalline silicon photo-transistor arrays detector, it is to use non-crystalline silicon tft (a-Si Thin Film Transistor)
Manufacture craft is made, and has PIN structural.
Amorphous silicon photodiodes detector array is to make work using non-crystalline silicon TFD (a-Si Thin Film Diode)
Made of skill.
Low temperature polycrystalline silicon photo-transistor arrays detector is to use low temperature polycrystalline silicon LTPS (Low Temperature
Poly-Silicon, p-Si) made of manufacture craft.
Crystalline silicon photo-transistor arrays detector is as materials'use integrated circuit technology system using monoblock silicon single crystal
Make.
It is highly preferred that the flat board probe unit uses photoelectron diode array detector.
Preferably, the probe unit also includes signal reading module, and it is single that the signal reads module connection flat board detection
Member.Signal, which reads module, can use low power consumption digital integrated circuit, and row pulse signal is converted into serial pulse signal.
Preferably, the flat board probe unit is encapsulated in protective clear layer.It is highly preferred that the protective clear layer can be with
From quartz glass or the glass through surface passivating treatment, the rigid plastics through surface passivating treatment.
Preferably, the pixel spacing of the flat board probe unit is 50um~300um.
Preferably, the flat board probe unit is rectangle, it is highly preferred that the rectangular shape is 6cm x 8cm；
Preferably, the length 10~20cm, wide 8~15cm, high 2~8cm of the magazine；
Another aspect of the present utility model provides a kind of electronic cassette preforming device for chemiluminescence detection, described
Electronic cassette preforming device includes the probe unit as described above for being used for chemiluminescence detection.
Preferably, the electronic cassette preforming device comprises at least：The power supply and control unit set in magazine, the control
Unit processed comprises at least main control module, data read module, data transmission module；The flat board probe unit connects main control
Module, the data read module and the data transmission module all connect the main control module, and the power supply is whole dress
Put power supply.
Preferably, the data read module includes A/D transform subblocks, described in the A/D transform subblocks connection
Flat board probe unit.
Preferably, the power supply, control unit are arranged in lower box body.
When the flat board probe unit is located in upper magazine, the power supply and described control unit are located at detection platform
Lower section.
Preferably, described control unit also includes software interface module, the software interface module and the master control molding
Block connects.
Preferably, described control unit also includes display module and input module, and the display module and input module connect
Connect main control module.
Further, lock is also set up on magazine, upper cartridge body and lower box body can be pinned.Main control module can be notified to lock
Or unlocking condition.
As described above, probe unit, the electronic cassette preforming device of the present utility model for chemiluminescence detection, has
Following beneficial effect：
Qualitative and quantitative point can be conveniently carried out to molecular engram film, especially chemiluminescence Western blotting film
Analysis.Flat panel detector used in the utility model can work at room temperature, be not required to auxiliary cooling, corresponding to the utility model
Detection operating process is not required to focus；It is simple in construction, small volume, in light weight, it is moved easily use.Its weight is about 1~3kg, general
Logical experimenter can move or carry, available for Site Detection.
Brief description of the drawings
Fig. 1 is shown as the utility model magazine structural representation.
Fig. 2 is shown as the utility model main control module structural framing figure.
Fig. 3 is shown as main control module structural framing figure in the utility model another embodiment.
Fig. 4 is shown as main control module structural framing figure in the utility model another embodiment.
Fig. 5 is shown as main control module structural framing figure in the utility model another embodiment.
Component label instructions
1 magazine
Magazine on 101
102 times magazines
103 USB interfaces
100 flat board probe units
200 control units
201 data read modules
2011 A/D transform subblocks
202 main control modules
203 data transmission modules
204 software interface modules
205 display modules
206 input modules
Embodiment
Embodiment of the present utility model is illustrated by particular specific embodiment below, those skilled in the art can be by this
Content disclosed by specification understands other advantages and effect of the present utility model easily.
Fig. 1 is referred to Fig. 5.It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to
Coordinate the content disclosed in specification, so that those skilled in the art understands and reads, be not limited to the utility model
Enforceable qualifications, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or size
Adjustment, in the case where not influenceing the effect of the utility model can be generated and the purpose that can reach, all should still fall in the utility model
Disclosed technology contents are obtained in the range of covering.Meanwhile in this specification it is cited as " on ", " under ", " left side ",
The term on " right side ", " centre " and " one " etc., understanding for narration is merely convenient to, and it is enforceable to be not used to restriction the utility model
Scope, its relativeness are altered or modified, enforceable when being also considered as the utility model in the case where changing technology contents without essence
Category.
Main operational principle of the present utility model is as follows：Blotting membrane and flat panel detector are pressed close to place, only retained certain
Space is to provide luminescence-producing reaction molecule (being usually luminol) to chemical luminous substrate, the effect of chemical luminous substrate.Blotting membrane
On protein band be combined with corresponding antibody, antibody carry horseradish peroxidase, horseradish peroxidase can catalytic chemistry send out
Luminol mulecular luminescence in light substrate, its luminous signal can closely conduct photodiode/crystalline substance to flat panel detector
Light intensity signal is converted to corresponding electronic signal, electronic signal transmission to computer shape by body pipe, photodiode/transistor
Into data signal.The data signal power be used to estimate correspondence position protein expression amount (protein is located at blotting membrane
On).
Such as Fig. 1, a kind of probe unit for chemiluminescence detection, the probe unit includes magazine 1, the magazine 1
Including upper cartridge body 101 and lower box body 102, the upper cartridge body 101 and the lower box body 102 match, and are horizontally disposed with the magazine 1
Flat board probe unit (not shown), the flat board probe unit horizontal do not have in magazine 1 on the flat board probe unit 1
There is cesium iodide coating.The flat board probe unit is non-crystalline silicon photoelectron diode array detector, and there is more preferable electric current to open for it
Close characteristic, reading speed faster, the higher (QE at 550nm wavelength of quantum yield>80%), photosensitive region fill factor is higher
(about 70%), naturally it is also possible to it is other, such as non-crystalline silicon photo-transistor arrays detector, low-temperature polysilicon silicon phototransistor
Any one in detector array or crystalline silicon photo-transistor arrays detector.
The flat board probe unit can be located in upper cartridge body 101 or lower box body 102；When the flat board probe unit position
In upper cartridge body 101, detection platform is additionally provided with the lower box body 102, is mainly used in placing tested substance, the detection is flat
Platform is flat on 1mm below flat board probe unit, in general can be with 1~2mm at, when the flat board probe unit is located at lower box body
Itself as detection platform when in 102, you can to place tested substance, while detected material can be detected.
In an embodiment, flat board detection unit is located in lower box body, the flat board detection platform it is photosensitive up, and
The periphery of photosurface sets projection, and the raised height is 1~2mm, described when flat board detection unit is located in upper cartridge body
The periphery of detection platform in lower box body is also provided with 1~2mm of height projection.When the raised purpose of the above is to prevent from using
The liquid chemical substance in flat board detection unit or detection platform is added in four circumfluences, while also there is support.
In an embodiment, when the flat board detection unit is placed in lower box body, flat panel detector it is photosensitive up, make
Used time, a certain amount of chemical luminous substrate (liquid) is added dropwise on the photosurface of flat panel detector, by blotting membrane back-off in photosensitive
On face, to keep having a certain amount of chemical luminous substrate between blotting membrane and photosurface, close and covered on tight device, to form lucifuge
Condition, upper lid should keep enough distances with blotting membrane, must not produce any pressure to blotting membrane.Upper cover locking device is locked, with
Ensure lucifuge state stable in detection process.In detection process, to keep cassette static, avoid blotting membrane and flat board
Relative position between detector changes.(this mode is referred to as lower cover pattern)
When the flat board detection unit is placed in upper cartridge body, detection platform is also set up in lower magazine.Detection platform has
There is the size consistent with flat panel detector, and be placed exactly in below flat board detection unit.For placing blotting membrane.Flat panel detector
It is photosensitive down, flat panel detector is connected by flexible circuit with the control unit in lower magazine.In detection process, blotting membrane
It is placed in magazine in detection platform, after addition chemical luminous substrate (liquid), upper cartridge body is closed, positioned at the flat board of upper cartridge body
Detector photosurface will be buckled on blotting membrane, keep blotting membrane with flat board protective layer apart from being 1~2mm by projection, to change
Learn luminous substrate and leave space, avoid expelling the substrate on blotting membrane.Chemical luminous substrate is full of trace because of surface tension
Between film and flat board protective layer, ensure being normally carried out for chemiluminescence reaction.After upper cartridge body closes, it is in tight with the wall of device four
Closely connected conjunction state, to ensure detection process without external light influence signal detection, in so-called lucifuge state.Then locking is locked
Device, to ensure that the relative position between detection process middle plateform detector and blotting membrane does not change, while also avoid brokenly
Bad lucifuge state (this mode is referred to as upper cover mold formula).
Further, signal is additionally provided with the magazine and reads module, the signal reads module connection flat board detection unit,
The signal, which reads module, can use circuit board, and row pulse signal is converted into serial pulse signal.
The flat board probe unit is rectangle, and photosensitive area is more than the size of usual blotting membrane, preferably uses 6cm x
8cm flat panel detector detector.The usual size of blotting membrane as detection object is 5cm x 6cm, can be covered all
Need the blotting membrane detected.The long 15cm of magazine, wide 10cm, high 6cm, in general, can be long 10~20cm, wide 8~15cm,
High 2~8cm.The pixel spacing of the flat board probe unit is 100um, typically can be 50um~300um.It is verified by experiments,
Final resolution ratio not less than 3.0lp/mm can be obtained using the flat panel detector, this is also needed for the detection of chemiluminescence blotting membrane
The base resolution wanted.The spatial resolution of digital measuring that flat panel detector obtains determines by Pixel size (distance), as
Member is bigger, and spatial resolution is lower；Pixel is smaller, and spatial resolution is higher, but can reduce photosensitive area fill factor, so as to cause
Sensitivity declines.
Preferably, the flat board probe unit is encapsulated in protective clear layer, avoids dust and chemical luminous substrate from polluting
Flat panel detector.The protective clear layer selects quartz glass, naturally it is also possible to from the glass through surface passivating treatment, through table
The rigid plastics of face Passivation Treatment.
Using flat board detection unit described in the utility model, whole detection unit can be placed on one it is less dark
In box, there is very big advantage relative to traditional CCD detectors.CCD limits (typically smaller than 1 English because its photosensitive area is smaller
It is very little), much smaller than the blotting membrane size (more than 7 inches) of required shooting, therefore CCD must be by can just gather after lens focus
Illuminated message on whole blotting membrane simultaneously carries out opto-electronic conversion and record.The flat panel detector used in the utility model is felt because of it
Light area is sufficiently large, can cover whole blotting membrane or multiple blotting membranes, then need not use lens focus, avoid optical signal
Reflection loss on lens, greatly improved detection sensitivity.It can at room temperature work, be not required to auxiliary cooling.
Detection of electrons signal is corresponded to the detection blotting membrane chemiluminescence of amorphous silicon photodiodes detector array below
Process illustrates detection process of the flat panel detector to chemiluminescence blotting membrane as example, and other flat panel detectors are to blotting membrane
Detection process has higher similarity：
1. before blotting membrane detection, the row drive wire of amorphous silicon photodiodes detector array produces one relatively with arranging electricity
The negative pulse of position, the negative pulse make switching diode turn on, photodiode capacitance are charged.
2. after the chemiluminescence irradiation on blotting membrane corresponds to pixel, its optical charge discharges electric capacity.
3. carrying out second of driving pulse again, photodiode capacitance is recharged, the amount of charge specifically to charge with
Optical charge quantity is corresponding.
Driven 4. the unified command of the time schedule controller on amorphous silicon photodiodes detector array is descending by pixel
Charging charge detects line by line, and signal reads module and is made up of low power consumption digital integrated circuit, and the circuit changes row pulse signal
For serial pulse signal.
As shown in Fig. 1~5, the utility model provides a kind of electronic cassette preforming device for chemiluminescence detection, bag
Containing the above-mentioned probe unit for chemiluminescence detection.Usb 1 03 is additionally provided with the side of magazine 1.
Such as Fig. 2, in the present embodiment, power supply, control unit 200, flat board probe unit are set in the lower box body 102
100, described control unit comprises at least main control module 202, data read module 201, data transmission module 203；It is described flat
Plate probe unit 100 connects main control module 202, and the data read module 201 and the data transmission module 203 all connect
The main control module 202, the power supply are powered for whole device.Certainly, the flat board detection unit can also be placed in box
In body.
Such as Fig. 3, in the present embodiment, the data read module 201 includes A/D transform subblocks 2011, the A/D
Transform subblock 2011 connects the flat board probe unit.Further, it is that connection signal reads module, A/D transform subblocks
Pulse signal is converted to data signal by 2011, is packaged into particular file format through back-end digital circuit, is passed through data transfer list
Member, which is transmitted to host computer, to be shown and quantitative analysis processing, such as can be spread out of data using USB interface.
Further, the accessory circuit of flat board probe unit can be also encapsulated in protective layer.
Such as Fig. 4, in the present embodiment, described control unit also includes software interface module 204, the software interface module
With the main control module, it can be set up and be connected with host computer by USB interface (or bluetooth, LAN) before, host computer
The exposure parameter setting of electronic cassette preforming device, exposure commands is controlled to assign using software by software interface module 204.
Such as Fig. 5, in another embodiment, described control unit also includes display module 205 and input module 206,
The display module 205 and input module 206 connect main control module.The display module and input module use touch-screen,
The main control module uses single-chip microcomputer, and therefore, user can utilize touch-screen to realize the control to the present apparatus.
In another embodiment, lock is also set up on magazine, upper cartridge body and lower box body can be pinned so that during test, energy
Enough it is guaranteed in the environment of dark.For further optimizing design scheme, the lock is connected with main control module, the master control
Molding block can detect that lock is in the state pinned or opened.
Those skilled in the art understand, and as described above, convert optical signal into the process of electric signal, obtain numeral letter
Number process, the process by digital data transmission, can utilize computer of the prior art, integrated circuit modules, can compile
Journey logical device, other hardware or existing software module are realized.So the utility model effectively overcomes in the prior art
Various shortcoming and have high industrial utilization.
Above-described embodiment only illustrative principle of the present utility model and its effect are new not for this practicality is limited
Type.Any person skilled in the art can all be carried out without prejudice under spirit and scope of the present utility model to above-described embodiment
Modifications and changes.Therefore, such as those of ordinary skill in the art without departing from the essence disclosed in the utility model
God and all equivalent modifications completed under technological thought or change, should be covered by claim of the present utility model.
Claims (9)
1. a kind of probe unit for chemiluminescence detection, it is characterised in that the probe unit includes magazine, the magazine
Middle setting flat board probe unit, for the flat board probe unit horizontal in magazine, the magazine includes upper cartridge body and lower box body, institute
Upper cartridge body and lower box body matching are stated, the flat board probe unit is located in upper cartridge body or lower box body；When the flat board is visited
When survey unit is located in upper cartridge body, detection platform is additionally provided with the lower box body, it is single that the detection platform is flat on flat board detection
At 1~2mm of first lower section；When the flat board probe unit is located in lower box body itself as detection platform.
2. the probe unit according to claim 1 for chemiluminescence detection, it is characterised in that the flat board detection is single
Member is brilliant selected from non-crystalline silicon photo-transistor arrays detector, amorphous silicon photodiodes detector array, low temperature polycrystalline silicon photoelectricity
Any one in body pipe detector array or crystalline silicon photo-transistor arrays detector.
3. the probe unit according to claim 1 for chemiluminescence detection, it is characterised in that the probe unit is also
Module is read including signal, the signal reads module connection flat board probe unit.
4. the probe unit according to claim 1 for chemiluminescence detection, it is characterised in that the flat board detection is single
Member is encapsulated in protective clear layer.
5. the probe unit according to claim 1 for chemiluminescence detection, it is characterised in that the flat board detection is single
Member also includes any one or a few in following technical characteristic：
(1) the flat board probe unit is rectangle；
(2) the length 10~20cm, wide 8~15cm, high 2~8cm of the magazine；
(3) the pixel spacing of flat board probe unit is 50um~300um.
A kind of 6. electronic cassette preforming device for chemiluminescence detection, it is characterised in that the electronic cassette preforming device
Include the probe unit for chemiluminescence detection as described in any one of Claims 1 to 5.
7. the electronic cassette preforming device according to claim 6 for chemiluminescence detection, it is characterised in that the electricity
Sub- magazine preforming device comprises at least：The power supply and control unit set in magazine, described control unit comprise at least master control
Molding block, data read module and data transmission module；The flat board probe unit connects main control module, the data
Read module and the data transmission module all connect the main control module, and the power supply is powered for whole device.
8. the electronic cassette preforming device according to claim 7 for chemiluminescence detection, it is characterised in that the number
Include A/D transform subblocks according to read module, the A/D transform subblocks connect the flat board probe unit.
9. the electronic cassette preforming device according to claim 7 for chemiluminescence detection, it is characterised in that the electricity
Source and control unit are arranged in lower box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720692364.XU CN206945543U (en) | 2017-06-14 | 2017-06-14 | Probe unit, electronic cassette preforming device for chemiluminescence detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720692364.XU CN206945543U (en) | 2017-06-14 | 2017-06-14 | Probe unit, electronic cassette preforming device for chemiluminescence detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206945543U true CN206945543U (en) | 2018-01-30 |
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ID=61365126
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| CN201720692364.XU Expired - Fee Related CN206945543U (en) | 2017-06-14 | 2017-06-14 | Probe unit, electronic cassette preforming device for chemiluminescence detection |
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| CN (1) | CN206945543U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085157A (en) * | 2017-06-14 | 2018-12-25 | 淳洁 | For the probe unit of chemiluminescence detection, electronic cassette preforming device and application thereof |
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2017
- 2017-06-14 CN CN201720692364.XU patent/CN206945543U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085157A (en) * | 2017-06-14 | 2018-12-25 | 淳洁 | For the probe unit of chemiluminescence detection, electronic cassette preforming device and application thereof |
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Granted publication date: 20180130 Termination date: 20200614 |