CN206298604U - A kind of drop culture apparatus of cell - Google Patents
A kind of drop culture apparatus of cell Download PDFInfo
- Publication number
- CN206298604U CN206298604U CN201621262089.XU CN201621262089U CN206298604U CN 206298604 U CN206298604 U CN 206298604U CN 201621262089 U CN201621262089 U CN 201621262089U CN 206298604 U CN206298604 U CN 206298604U
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- culture
- hole
- cell
- cultivation region
- microns
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Abstract
The utility model provides a kind of drop culture apparatus of cell, it includes culture dish, the bottom of the culture dish is provided with cultivation region, the cultivation region includes the first cultivation region and the second cultivation region, first cultivation region is provided with the first culture hole, and second cultivation region is provided with the second culture hole, and first culture hole is tip hole, the bottom of second culture hole is flat, and the oral area internal diameter of second culture hole is more than bottom inner diameter.Using the technical solution of the utility model, cell is cultivated in being put into culture hole, facilitating cell carries out drop culture, make each cell that some biotic factors are discharged in incubation, act on each other, play a part of to promote growth, and combine the growth phase of cell, coordinate hole of different shapes, the effect of cell culture is more preferable.
Description
Technical field
The utility model is related to a kind of cell culture apparatus, more particularly to a kind of cell drop culture apparatus.
Background technology
At present, the culture to cell is typically carried out using surface plate, and the embryo transfer of blastula stage is for Embryonic limb bud cell
The raising of rate is extremely important, but the in vitro culture of blastaea needs the culture systems of efficient stable, and drop cultivation can be solved
This problem.But, for drop cultivation, current culture apparatus is difficult control, and stability is bad.
Utility model content
For above technical problem, the utility model provides a kind of drop culture apparatus of cell, is conducive to carrying out liquid
Drop culture, makes each cell that some biotic factors are discharged in incubation, acts on each other, plays a part of to promote growth.
In this regard, the technical solution of the utility model is：
A kind of drop culture apparatus of cell, it includes culture dish, and the bottom of the culture dish is provided with cultivation region, the training
Supporting area includes the first cultivation region and the second cultivation region, and first cultivation region is provided with the first culture hole, and second cultivation region sets
There is the second culture hole, first culture hole is tip hole, and the bottom of second culture hole is flat, second culture hole
Oral area internal diameter be more than bottom inner diameter.Wherein, second culture hole is used for cell culture in 0-1 days and checks whether cell is sent out
Educate normal, the first culture hole was the place of real cultured cells, for the culture of the 1-5 days.Using this technical scheme, by cell
It is put into culture hole, nutrient solution is then added in culture dish, carries out drop culture, culture of each cell in 3 D stereo
In pendular ring border（Aperture）Culture, but each cell discharges some biotic factors in incubation and enters in culture dish, each other
Effect, plays a part of to promote growth；And the growth phase of combination cell, coordinate hole of different shapes, be conducive to cell
Culture.
Used as further improvement of the utility model, the depth of the culture hole is 200-400 microns.
Preferably, the bottom of first culture hole is the pointed shape of similar leaf.
Preferably, the quantity of first culture hole and the second culture hole is at least two.
Used as further improvement of the utility model, first culture hole is inverted cone shape.
Used as further improvement of the utility model, first culture hole, the inwall of the second culture hole are outwards recessed
Cambered surface.
Used as further improvement of the utility model, the oral area internal diameter of first culture hole is 20-400 microns；It is described
The oral area internal diameter of the second culture hole is 20-400 microns, and bottom inner diameter is 200-300 microns.
Used as further improvement of the utility model, the oral area internal diameter of first culture hole is 150-300 microns；It is described
The oral area internal diameter of the second culture hole is 200-400 microns, and bottom inner diameter is 100-300 microns.Using this technical scheme, more favorably
In cell culture.
Used as further improvement of the utility model, first culture hole, the depth of the second culture hole are that 150-300 is micro-
Rice.
Used as further improvement of the utility model, the quantity of the culture hole of the cultivation region is at least 2, the culture
The distance between hole is not less than 80 microns.
Preferably, the quantity of the culture hole of the cultivation region is 16, anyhow each 4 apertures.
Used as further improvement of the utility model, the quantity of the cultivation region is two or more.
Used as further improvement of the utility model, the bottom of the culture dish is additionally provided with cell and rinses boss.It is described thin
The effect that born of the same parents rinse boss is used to rinse cell, and number is not limited, and at least one.
Compared with prior art, the beneficial effects of the utility model are：
Using the technical solution of the utility model, cell is cultivated in being put into culture hole, facilitates cell to carry out drop training
Support, make each cell that some biotic factors are discharged in incubation, act on each other, play a part of to promote growth, Er Qiejie
The growth phase of cell is closed, coordinates hole of different shapes, the effect of cell culture is more preferable.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model embodiment 1.
Fig. 2 is the structural representation of the first culture hole of the utility model embodiment 1.
Fig. 3 is the structural representation of the second culture hole of the utility model embodiment 1.
Fig. 4 is the structural representation of the utility model embodiment 2.
Specific embodiment
Below in conjunction with the accompanying drawings, preferably embodiment of the present utility model is described in further detail.
Embodiment 1
As shown in Fig. 1 ~ Fig. 3, a kind of drop culture apparatus of cell, it includes culture dish 1, the bottom of the culture dish 1
It is provided with cultivation region and cell rinses boss 4, culture hole is provided with the cultivation region, the cultivation region includes the He of the first cultivation region 2
It is provided with second cultivation region 3, first cultivation region 2 in first culture hole 21, second cultivation region 3 and is provided with the second culture hole
31.First culture hole 21 is inverted sharp bottom, substantially circular cone body opening, and similar point is lobate, as shown in Fig. 2 first training
The oral area internal diameter for supporting hole 21 is 250 microns.The bottom of second culture hole 31 is flat, the oral area of second culture hole 31
Internal diameter is more than bottom inner diameter, and the oral area internal diameter of second culture hole 31 is 300 microns, and bottom inner diameter is 200 microns.Described
One culture hole 21, the depth of the second culture hole 31 are 250 microns.The bottom of the culture dish 1 is additionally provided with two cells and rinses convex
Platform 4.The quantity of the first culture hole 21 is 16 in first cultivation region 2, horizontally-arranged and each four of vertical setting of types, first culture hole
The distance between 21 are not less than 80 microns.The quantity of the second culture hole 31 in second cultivation region 3 is 16, horizontally-arranged and perpendicular
Each four are arranged, the distance between described second culture hole 31 is not less than 80 microns.
Embodiment 2
On the basis of embodiment 1, as shown in figure 4, the bottom of the culture dish 1 is provided with four cultivation regions, the first culture
2 and second cultivation region of area 3 each two；The bottom of the culture dish 1 is additionally provided with two cells and rinses boss 4, first culture
The quantity of the first culture hole 21 is 16 in area 2, horizontally-arranged and each four of vertical setting of types, and the distance between described first culture hole 21 is 80
Micron.The quantity of the second culture hole 31 in second cultivation region 3 is 16, horizontally-arranged and each four of vertical setting of types, second training
It is 80 microns to support the distance between hole 31.The specific embodiment of the above is better embodiment of the present utility model, and
Non- to limit specific implementation scope of the present utility model with this, scope of the present utility model includes being not limited to this specific embodiment party
Formula, the equivalence changes that all shapes according to the utility model, structure are made are in protection domain of the present utility model.
Claims (10)
1. the drop culture apparatus of a kind of cell, it is characterised in that：It includes culture dish, and the bottom of the culture dish is provided with culture
Area, the cultivation region includes the first cultivation region and the second cultivation region, and first cultivation region is provided with the first culture hole, described second
Cultivation region is provided with the second culture hole, and first culture hole is tip hole, the bottom of second culture hole be it is flat, described the
The oral area internal diameter of two culture holes is more than bottom inner diameter.
2. the drop culture apparatus of cell according to claim 1, it is characterised in that：The depth of the culture hole is 200-
400 microns.
3. the drop culture apparatus of cell according to claim 1, it is characterised in that：First culture hole is inverted
Cone shape.
4. the drop culture apparatus of cell according to claim 1, it is characterised in that：First culture hole, the second training
The inwall for supporting hole is outwards recessed cambered surface.
5. the drop culture apparatus of the cell according to claim 1 ~ 4 any one, it is characterised in that：First culture
The oral area internal diameter in hole is 20-400 microns；The oral area internal diameter of second culture hole is 20-400 microns, and bottom inner diameter is 200-
300 microns.
6. the drop culture apparatus of cell according to claim 5, it is characterised in that：In the oral area of first culture hole
Footpath is 150-300 microns；The oral area internal diameter of second culture hole is 200-400 microns, and bottom inner diameter is 150-300 microns.
7. the drop culture apparatus of cell according to claim 6, it is characterised in that：First culture hole, the second training
The depth for supporting hole is 150-300 microns.
8. the drop culture apparatus of cell according to claim 5, it is characterised in that：The number of the culture hole of the cultivation region
Amount is at least 2, and the distance between described culture hole is not less than 80 microns.
9. the drop culture apparatus of cell according to claim 5, it is characterised in that：The quantity of the cultivation region is two
More than.
10. the drop culture apparatus of the cell according to claim 1 ~ 4 any one, it is characterised in that：The culture dish
Bottom be additionally provided with cell rinse boss.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621262089.XU CN206298604U (en) | 2016-11-18 | 2016-11-18 | A kind of drop culture apparatus of cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621262089.XU CN206298604U (en) | 2016-11-18 | 2016-11-18 | A kind of drop culture apparatus of cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206298604U true CN206298604U (en) | 2017-07-04 |
Family
ID=59211420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621262089.XU Active CN206298604U (en) | 2016-11-18 | 2016-11-18 | A kind of drop culture apparatus of cell |
Country Status (1)
| Country | Link |
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| CN (1) | CN206298604U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108251353A (en) * | 2018-01-22 | 2018-07-06 | 深圳韦拓生物科技有限公司 | A kind of Human embryo in vitro culture liquid and culture systems |
-
2016
- 2016-11-18 CN CN201621262089.XU patent/CN206298604U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108251353A (en) * | 2018-01-22 | 2018-07-06 | 深圳韦拓生物科技有限公司 | A kind of Human embryo in vitro culture liquid and culture systems |
| CN108251353B (en) * | 2018-01-22 | 2020-12-08 | 深圳韦拓生物科技有限公司 | Human embryo in-vitro culture solution and culture system |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 518000 Room 601, building B, haikexing strategic emerging industrial park, No. 16, Baoshan Road, Pingshan street, Pingshan District, Shenzhen, Guangdong Patentee after: SHENZHEN VITAVITRO BIOTECH Co.,Ltd. Address before: 518000 Room 501, building B, haikexing strategic emerging industrial park, No. 16, Baoshan Road, Pingshan street, Pingshan new area, Shenzhen, Guangdong Patentee before: SHENZHEN VITAVITRO BIOTECH Co.,Ltd. |