CN206215248U - A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding - Google Patents
A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding Download PDFInfo
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- CN206215248U CN206215248U CN201621194400.1U CN201621194400U CN206215248U CN 206215248 U CN206215248 U CN 206215248U CN 201621194400 U CN201621194400 U CN 201621194400U CN 206215248 U CN206215248 U CN 206215248U
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Abstract
The utility model discloses a kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding, including cover plate and substrate, the center of the cover plate is machined with liquid storage tank and Obstruct membrane, along the circumferential direction partially it is uniformly distributed on the upper surface of the substrate and is machined with some groups of biological detection fine structures, the cover plate and substrate encapsulate closed under vacuum or hot saturated vapor, when micro-fluidic chip is moved back under normal temperature and pressure, the fine structure inside has certain negative pressure, when microfluid enters fine structure by liquid storage tank, because the presence of pressure difference being capable of spontaneous vasomotion, realize that micro-fluidic chip liquid mixes, the function of biological detection.When under the utility model normal temperature and pressure, fine structure inside have certain negative pressure, microfluid enter fluid injection pond after, due to pressure difference presence can spontaneous vasomotion, realize micro-fluidic chip liquid mixing, the function of biological detection.
Description
Technical field
The utility model is related to a kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding, especially with miniflow
The micro-fluidic chip of pressure official post microfluid self-discipline flowing inside and outside road.
Background technology
Micro-fluidic chip is extension of the chip in fields such as biochemistries, by Micrometer-Nanometer Processing Technology, sequencing analysis technology
And digital information technology, realize the work(such as mixing in biochemistry, separation, chemical composition analysis, gene sequencing and Viral diagnosis
Energy.Application of the micro-fluidic chip in terms of biochemistry detection technique has that sample requirements are few, analyze speed fast, is easy to carry
The features such as, it is especially suitable for analysis in real time.In micro-meter scale, capillarity is microfluid is restrained oneself in fluid channel the important of flowing
Factor.In the runner of micro-fluidic chip, by changing the matrix of chip, fluid channel can be made（100~500μm）To microfluid
Absorption affinity is produced, the surface tension of microfluid is further excited, such that it is able to guiding or hindering the flowing of microfluid, realized micro-
The control of flow of fluid.The mode that current micro-fluidic chip driving microfluid flows in chip has pressure-driven, Pneumatic Micropump
Drive, centrifugation driving and driven by electroosmosis etc. are high to machinery equipment precision, cost requirement, are unfavorable for carrying and real-time detection.Separately
Outward, sample and detection liquid are introduced by external drive in current chip, it is difficult to realize mass.
Utility model content
The purpose of this utility model is to design a kind of microfluid spontaneous vasomotion micro-fluidic chip of new negative pressure guiding,
Operation to external world and the dependence of instrument and equipment are reduced, the quick flowing of microfluid stabilization in the fluid channel, quick in reaction tank is realized
The purpose for efficiently mixing.
The utility model solves the technical scheme that its technical problem used：
A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding, including cover plate and substrate, in the cover plate
Heart position is machined with liquid storage tank and Obstruct membrane, if be along the circumferential direction partially uniformly distributed on the upper surface of the substrate being machined with
A dry group biological detection fine structure, the cover plate and substrate encapsulated under vacuum or hot saturated vapor it is closed, when micro-fluidic
Chip is moved back to when under normal temperature and pressure, and the fine structure inside has certain negative pressure, and microfluid enters fine knot by liquid storage tank
During structure, due to pressure difference presence can spontaneous vasomotion, realize micro-fluidic chip liquid mixing, the function of biological detection.
Further, the material of the cover plate is EVOH material transparent films, height value h1Between 2mm ~ 4mm.
Further, the liquid storage tank uses cylindrical structure, diameter to be directly proportional to the volume size of reaction blood sample.
Further, baseplate material is quartz glass, length and width value between 60mm ~ 80mm, height value h25mm ~
Between 10mm.
Further, the fine structure described in every group includes fluid injection pond, sprue, reaction tank, flow channel and gas storage pond,
The fluid injection pond is located at upper surface of base plate center, and the sprue is communicated between fluid injection pond and reaction tank, the air-flow
Between road connected reaction pond and gas storage pond.
Further, described fluid injection pond uses cylindrical structure, basal diameter between 4mm ~ 6mm, highly at 300 μm
Between ~ 500 μm.
Further, the reaction tank uses cylindrical structure, basal diameter between 5mm ~ 8mm, highly 500 μm ~
Between 800 μm, the volume size of reaction tank is proportional with reaction blood sample volume size.
Further, the gas storage pond uses cylindrical structure, basal diameter between 12mm ~ 16mm, highly at 800 μm
Between ~ 1000 μm, the volume size in the gas storage pond is directly proportional to the size of microfluidic flow speed needed for experiment.
Further, using the symmetrical and mutually angled V-structure in both sides, angle change scope exists the sprue
Between 60 ° ~ 90 °, depth value is between 300 μm ~ 500 μm and depth is identical everywhere.
Further, using the symmetrical and mutually angled V-structure in both sides, angle change scope exists the flow channel
Between 60 ° ~ 90 °, there is gradient in the depth of flow channel, and from reaction tank side to gas storage pond side, depth gradually increases and gradient
It it is 0.1 ° ~ 0.8 °, it is ensured that reaction solution will not enter into gas storage pond.
Compared to existing technology, the cover plate of the present utility model and substrate encapsulate close under vacuum or hot saturated vapor
Close, due to the effect in gas storage pond on substrate, when micro-fluidic chip is moved back under normal temperature and pressure, fluid injection pond, sprue, in reaction tank
Portion has certain negative pressure, and microfluid enters after fluid injection pond, due to pressure difference presence can spontaneous vasomotion, realize micro-fluidic core
The mixing of piece liquid, the function of biological detection.Microfluid streamlined flow, the speed of liquid mixing are determined that pressure difference can be with by pressure difference
Changed by adjusting the volume of micro-structural on hot saturated-steam temperature and adjustment substrate.A kind of microfluid self-discipline of negative pressure guiding
The micro-fluidic chip of motion, can shift to an earlier date pre-buried detection reagent, be preserved for a long time, and outside inspection can be accurately introduced when using
Test sample sheet, is easy to implement integrated chip and industrialization.
Brief description of the drawings
The micro-fluidic chip three-dimensional appearance schematic diagram that Fig. 1 is designed for the utility model.
The micro-fluidic chip cross section structure schematic diagram that Fig. 2 is designed for the utility model.
The micro-fluidic chip substrate three-dimensional appearance schematic diagram that Fig. 3 is designed for the utility model.
The micro-fluidic chip fluid channel cross section structure schematic diagram that Fig. 4 is designed for the utility model.
Shown in figure：1- substrates；2- cover plates；3- liquid storage tanks；4- Obstruct membranes；5- fluid injections pond；6- sprues；7- reaction tanks；
8- flow channels；9- gas storage pond.
Specific embodiment
In order to be best understood from the utility model, the utility model is done further with reference to the accompanying drawings and examples
It is bright, but the claimed scope of the utility model is not limited to the scope that embodiment is represented.
As depicted in figs. 1 and 2, a kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding, the micro-fluidic chip
Collectively constituted by substrate 1 and cover plate 2, substrate 1 is the main part of micro-fluidic chip, using rectangular structure, length and width size is
According to the number of sprue 6（4、6、8）Increase and increase, length and width value between 60mm ~ 80mm, height value h25mm ~
Between 10mm, material is quartz glass plate, and it is machined with a series of fine structures for biological detection above.The length of cover plate 2
It is roomy small identical with the length and width size of substrate 1, height value h1Between 2mm ~ 4mm, material is EVOH transparent membranes, lid
The center of plate 2 is machined with liquid storage tank 3 and Obstruct membrane 4.Liquid storage tank 3 uses cylindrical structure, and its size is according to reaction blood
The volume size of sample and change.Obstruct membrane 4 is used for separating liquid storage tank 3 and fluid injection pond 5 temporarily, when reaction starts, by special tool
Tool is punctured, and the blood sample in liquid storage tank 3 is injected into micro-fluidic chip.
As shown in Figure 3 and Figure 4, some groups of biological detection fine structures are machined with the upper surface of substrate 1, four are arranged in
Above decile or six deciles or eight equal parts angle of circumference, the present embodiment has four groups, and each group of fine structure is by fluid injection pond 5, main flow
Road 6, reaction tank 7, flow channel 8 and gas storage pond 9 collectively constitute, and one group of fine structure is used for a biological testing process.Fluid injection pond 5
Using cylindrical structure, basal diameter highly between 300 μm ~ 500 μm, for connecting liquid storage tank 3, is incited somebody to action between 4mm ~ 6mm
Blood sample is directed in the middle of micro flow chip.Sprue 6 is using the symmetrical α=β in both sides and mutually angled V-structure, angle
Excursion is between 60 ~ 90 °.The depth of sprue does not exist gradient, and consistent to reaction tank depth from fluid injection pond, value exists
Between 300 μm ~ 500 μm.Reaction tank 7 equally uses cylindrical structure, basal diameter between 5mm ~ 8mm, highly 500 μm ~
Between 800 μm, the volume size of reaction tank 7 changes with blood sample volume size is reacted.The pre-buried chemistry examination in the bottom of reaction tank 7
Agent, for medicine, liquid biology hybrid detection.Flow channel 8 is used for coupled reaction pond 7 and gas storage pond 9, for guiding negative-pressure gas, adopts
With the symmetrical α=β in both sides and mutually angled V-structure, angle change scope is between 60 ~ 90 °.The depth of flow channel 8 is deposited
In gradient, from reaction tank side to gas storage pond side, depth gradually increases, and gradient is 0.1 ~ 0.8 °, it is ensured that reaction solution will not enter
Enter to gas storage pond.Gas storage pond 9 uses cylindrical structure, basal diameter between 12mm ~ 16mm, highly 800 μm ~ 1000 μm it
Between, the size of its overall volume directly affects the vacuum magnitude inside micro-fluidic chip, the microfluidic flow speed according to needed for experiment
The size of degree and accordingly adjust its volume size.
Substrate 1 and cover plate 2 encapsulate closed under vacuum or hot saturated vapor, and reaction tank 7 and the inside of gas storage pond 9 have one
Fixed negative pressure.Microfluid enter fluid injection pond 5 after, can under the suction function in reaction tank 7 and gas storage pond 9 by sprue 6 to
The self-discipline of reaction tank 7 is flowed, and concrete operations are：
In aseptic dry environment, after micro-fluidic chip is cleaned with drug disinfection, it is placed in aseptic drying heater
100 degrees Celsius are heated to, while the antivirus that reaches a high temperature is acted on, the expansion using vapor and hot-air makes microchannel
The pressure on surface is reduced, and after temperature reaches 100 degrees Celsius, keeping temperature is simultaneously dried 3 ~ 5 minutes, is then taken out, and is placed on aseptic
Dry work indoor, pre-buried biological reagent, and in 10-20s with aseptic dry EVOH material transparents film cover plate 2 to substrate
1 is packaged laminating, gives free rein to be cooled under dry environment untill temperature no longer changes, so that chip internal runner
Pressure forms negative pressure relative to atmospheric pressure.Blood sample is placed in liquid storage tank 3, using special equipment, Obstruct membrane is punctured
4, blood sample is flowed into inside micro-fluidic chip, starts a series of biological testing process.
Above-described embodiment of the present utility model is only intended to clearly illustrate the utility model example, and is not
Restriction to implementation method of the present utility model.For those of ordinary skill in the field, on the basis of described above
On can also make other changes in different forms.There is no need and unable to be exhaustive to all of implementation method.
All any modification, equivalent and improvement made within spirit of the present utility model and principle etc., should be included in this reality
Within new scope of the claims.
Claims (10)
1. the micro-fluidic chip of the microfluid spontaneous vasomotion of a kind of negative pressure guiding, including cover plate (2) and substrate (1), its feature exist
In：The center of the cover plate (2) is machined with liquid storage tank (3) and Obstruct membrane (4), along circle on the upper surface of the substrate (1)
Circumferential direction is partially uniformly distributed and is machined with some groups of biological detection fine structures, and the cover plate (2) and substrate (1) are in vacuum
Or encapsulate closed under hot saturated vapor, when micro-fluidic chip is moved back under normal temperature and pressure, fine structure inside has one
Fixed negative pressure, microfluid by liquid storage tank (3) enter fine structure when, due to pressure difference presence can spontaneous vasomotion, realize miniflow
The mixing of control chip liquid, the function of biological detection.
2. according to claim 1 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：The lid
The material of plate (2) is EVOH material transparent films, height value h1Between 2mm ~ 4mm.
3. according to claim 1 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：The storage
Liquid pool (3) uses cylindrical structure, diameter to be directly proportional to the volume size of reaction blood sample.
4. according to claim 1 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：The base
Plate (1) material be quartz glass, length and width value between 60mm ~ 80mm, height value h2Between 5mm ~ 10mm.
5. according to claim 1 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：Every group of institute
The fine structure stated includes fluid injection pond (5), sprue (6), reaction tank (7), flow channel (8) and gas storage pond (9), the fluid injection
Pond (5) is communicated between fluid injection pond (5) and reaction tank (7) positioned at substrate (1) upper surface center, the sprue (6), institute
State between flow channel (8) connected reaction pond (7) and gas storage pond (9).
6. according to claim 5 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：Described
Fluid injection pond (5) uses cylindrical structure, basal diameter between 4mm ~ 6mm, highly between 300 μm ~ 500 μm.
7. according to claim 5 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：It is described anti-
Ying Chi (7) uses cylindrical structure, basal diameter between 5mm ~ 8mm, highly between 500 μm ~ 800 μm, reaction tank (7)
Volume size is proportional with reaction blood sample volume size.
8. according to claim 5 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：The storage
Gas pond (9) uses cylindrical structure, basal diameter between 12mm ~ 16mm, highly between 800 μm ~ 1000 μm, the gas storage
The volume size in pond (9) is directly proportional to the size of microfluidic flow speed needed for experiment.
9. according to claim 5 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：The master
Runner (6) using the symmetrical and mutually angled V-structure in both sides, angle change scope between 60 ° ~ 90 °, depth value
Between 300 μm ~ 500 μm and depth is identical everywhere.
10. according to claim 5 negative pressure guiding microfluid spontaneous vasomotion micro-fluidic chip, it is characterised in that：It is described
Flow channel (8) using the symmetrical and mutually angled V-structure in both sides, angle change scope between 60 ° ~ 90 °, flow channel
(8) there is gradient in depth, from reaction tank (7) side to gas storage pond (9) side, depth gradually increase and gradient be 0.1 ° ~
0.8°。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621194400.1U CN206215248U (en) | 2016-11-07 | 2016-11-07 | A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621194400.1U CN206215248U (en) | 2016-11-07 | 2016-11-07 | A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding |
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| CN206215248U true CN206215248U (en) | 2017-06-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201621194400.1U Withdrawn - After Issue CN206215248U (en) | 2016-11-07 | 2016-11-07 | A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guiding |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106391153A (en) * | 2016-11-07 | 2017-02-15 | 华南理工大学 | Micro-fluidic chip with micro-fluid in autonomous movement guided by negative pressures |
| CN107308996A (en) * | 2017-06-21 | 2017-11-03 | 浙江大学 | A kind of method that long-time maintains micro flow control chip negative pressure state |
| CN114345430A (en) * | 2022-01-14 | 2022-04-15 | 北京工商大学 | Portable device for simultaneously detecting multiple antibiotic residues through paper-based micro-fluidic chip |
| WO2023087179A1 (en) * | 2021-11-17 | 2023-05-25 | 深圳华大生命科学研究院 | Detection chip assembly tool, liquid injection apparatus and method, electronic device, and medium |
-
2016
- 2016-11-07 CN CN201621194400.1U patent/CN206215248U/en not_active Withdrawn - After Issue
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106391153A (en) * | 2016-11-07 | 2017-02-15 | 华南理工大学 | Micro-fluidic chip with micro-fluid in autonomous movement guided by negative pressures |
| CN106391153B (en) * | 2016-11-07 | 2019-04-09 | 华南理工大学 | A kind of micro-fluidic chip of the microfluid spontaneous vasomotion of negative pressure guidance |
| CN107308996A (en) * | 2017-06-21 | 2017-11-03 | 浙江大学 | A kind of method that long-time maintains micro flow control chip negative pressure state |
| CN107308996B (en) * | 2017-06-21 | 2020-03-17 | 浙江大学 | Method for maintaining negative pressure state of micro-fluidic chip for long time |
| WO2023087179A1 (en) * | 2021-11-17 | 2023-05-25 | 深圳华大生命科学研究院 | Detection chip assembly tool, liquid injection apparatus and method, electronic device, and medium |
| CN114345430A (en) * | 2022-01-14 | 2022-04-15 | 北京工商大学 | Portable device for simultaneously detecting multiple antibiotic residues through paper-based micro-fluidic chip |
| CN114345430B (en) * | 2022-01-14 | 2022-10-28 | 北京工商大学 | Portable device for simultaneously detecting multiple antibiotic residues through paper-based micro-fluidic chip |
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| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170606 Effective date of abandoning: 20190409 |