CN205750086U - A kind of STED super-resolution microscopic system of volume holographic reduction scanning light beam - Google Patents
A kind of STED super-resolution microscopic system of volume holographic reduction scanning light beam Download PDFInfo
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- CN205750086U CN205750086U CN201620666673.5U CN201620666673U CN205750086U CN 205750086 U CN205750086 U CN 205750086U CN 201620666673 U CN201620666673 U CN 201620666673U CN 205750086 U CN205750086 U CN 205750086U
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Abstract
The utility model discloses the STED super-resolution microscopic system of a kind of volume holographic reduction scanning light beam, including exciting light recording equipment, exciting light interference information is recorded on volume holographic material；Consume optical recorder, consumption interference of light information is recorded on volume holographic material；Scanning light beam reduction apparatus, by the exciting light being recorded on volume holographic material and the optical information reduction consuming light；Polarization components, the exciting light being reduced and consumption light become line polarized light through polarization components；First light beam output precision, for transmission or reflection light beam；Two-dimensional scanning mirrors device, makes described exciting light and consumes light swing, it is achieved two-dimensional scan；Microcobjective, to imaging of samples；Detection light path；Display and processing unit, for showing sample image and controlling described two-dimensional scanning mirrors device, use this utility model can solve existing STED microtechnique owing to the factors such as extraneous vibrations, the temperature difference cause exciting light and consume the problem that the focal beam spot registration of light is low.
Description
Technical field
This utility model relates to medical apparatus and instruments, the STED super-resolution of a kind of volume holographic reduction scanning light beam
Microscopic system.
Background technology
It is to sweep copolymerization Jiao that stimulated radiation consumes (Stimulated Emission Depletion, STED) microtechnique
On the basis of retouching microscopy, introduce the consumption light of 0 to 2 π vortex phase modulation, form hollow type hot spot and exciting light in focus
Speckle overlaps.By stimulated radiation principle, consume exciting light spot edge fluorescence, compress fluorescence facula area so that excite hot spot chi
Very little breakthrough diffraction limit, it is achieved super-resolution.Therefore, far field super-resolution and the STED of sectional slice scanning imagery feature it are provided simultaneously with
Microscopy, is widely used in the fields such as bioscience.Owing to STED microtechnique needs exciting light and consumes the focusing light of light
Speckle all highly overlaps in the horizontal on axially, and the dislocation at two focal beam spot centers can have a strong impact on super-resolution effect, therefore, be
System needs to introduce accurate angular adjustment device during building and debugging, and process of debugging is loaded down with trivial details, and still needs to before use
To check and to adjust the registration of two hot spots hand or with automatic module, the factor such as extraneous vibrations, the temperature difference all can cause
Two beam and focus misaligned.
Therefore, prior art has yet to be improved and developed.
Utility model content
The purpose of this utility model be to provide a kind of volume holographic reduction scanning light beam STED super-resolution microscopic system and
Microscopic method, solves the below technical problem that 1, existing STED microtechnique causes swashing due to factors such as extraneous vibrations, the temperature difference
Luminous and consumption light focal beam spot registration is low.2, STED microscopic system needs to introduce precision during building and debugging
Angular adjustment device, and process of debugging is loaded down with trivial details, remains a need for checking and adjust two light hand or with automatic module before using
The registration of speckle.
The technical solution of the utility model is as follows:
The STED super-resolution microscopic system of a kind of volume holographic reduction scanning light beam, wherein, including exciting light recording equipment, sends out
Penetrate exciting light and exciting light interference information is recorded on volume holographic material；
Consume optical recorder, launch exciting light and consume light and the interference information of two-beam is recorded in volume holographic material
On；(being on same position on volume holographic material, have recorded two-beam successively)
Scanning light beam reduction apparatus, launches exciting light and consumes light by the exciting light being recorded on volume holographic material and consumption
The optical information reduction of light；
Polarization components, the exciting light being reduced and consumption light become line polarized light through polarization components；
First light beam output precision, for transmission or reflection light beam；
Two-dimensional scanning mirrors device, makes described exciting light and consumes light swing, it is achieved the two-dimensional scan to sample；
Microcobjective, focuses on exciting light and consumption light on sample；
Detection light path, including a photodetector, is converted into the signal of telecommunication the optical signal of the sample light returned from sample；
Display and processing unit, be used for showing sample image and controlling described two-dimensional scanning mirrors device to realize sample
Two-dimensional scan.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described exciting light recording equipment
Including excitation light emission device, the second light beam output precision, coupling assembly, single-mode fiber, optical fiber collimator, beam splitting assembly, reflection
Assembly and volume holographic data board, described excitation light emission device sends excitation beam, and excitation beam is through described second light beam output
Transmitting in being coupled into described single-mode fiber by described coupling assembly after assembly transmission, the excitation beam of single-mode fiber exit end passes through
By described beam splitting components reflecting part transmission after optical fiber collimator collimation, reflection light beam by after reflection subassembly again secondary reflection with
Transmitted light beam produces interferes, and the interference information of formation is recorded on described volume holographic data board.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described consumption optical recorder
Including consuming optical transmitting set, the second light beam output precision, coupling assembly, single-mode fiber, optical fiber collimator, beam splitting assembly, reflection
Assembly, 0 to 2 π vortex phase plate and volume holographic data board, described consumption optical transmitting set sends consumption light beam, consumes light beam and passes through
Transmit in being coupled into described single-mode fiber by described coupling assembly after described second light beam output precision reflection, single-mode fiber outgoing
The consumption light beam of end is by the reflecting part transmission of beam splitting components after optical fiber collimator collimates, and transmitted light beam light beam is through 0
Forming phase-modulation light beam to 2 π vortex phase plates, reflection light beam light beam is produced with transmitted light beam by after reflection subassembly again secondary reflection
Interfering, the interference information of formation is recorded on described volume holographic data board.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described scanning light beam is the most original-pack
Put and include excitation light emission device, consumption optical transmitting set, the second light beam output precision, coupling assembly, single-mode fiber, optical fiber collimator
With volume holographic data board, excitation light emission device and luminescence while of consuming optical transmitting set, excitation beam and consumption light beam through described
After second light beam output precision merged, light beam after merging transmits in being coupled into described single-mode fiber by described coupling assembly,
The light beam of single-mode fiber exit end irradiates described volume holographic data board after optical fiber collimator collimates.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described two-dimensional scanning mirrors fills
Putting and include X-axis scanning galvanometer, Y axis scanning galvanometer and a galvanometer controller, described galvanometer controller controls described X-axis scanning galvanometer
Swing around X-direction, control Y axis scanning galvanometer and swing to realize the two-dimensional scan to sample around Y direction.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, also includes relaying light path, described
Relaying light path includes the object focus of the first lens and the second lens, the rear focus of described first lens and described second lens
Overlap.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described first light beam output group
Achromatism 1/2nd wave plate it is additionally provided with, described second lens and described micro-between part and described two-dimensional scanning mirrors device
Achromatic quarter waveplate it is provided with between object lens.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described first light beam output group
Part is fluorescence dichroic mirror.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described second light beam output group
Part is dichroic mirror.
The STED super-resolution microscopic system of described volume holographic reduction scanning light beam, wherein, described detection light path also includes
Only allow and return the optical filter that sample light passes through and the 3rd lens that return sample optical coupling is entered multimode fibre, return sample light quilt
Described photodetector it is transferred to after being coupled into multimode fibre.
The beneficial effects of the utility model: this utility model surpasses by providing the STED of a kind of volume holographic reduction scanning light beam
Differentiate microscopic system, utilize volume holographic technology, the consumption light beam after the excitation beam that height overlaps and 0～2 π phase-modulations
Record is on volume holographic material, when utilizing reference light reversely incident volume holographic material, and can be by two light beam reduction, two be reduced
Beam heights overlaps, and solves the beam drift brought due to extraneous vibration, temperature difference well and causes the problem that registration is low,
Eliminating the step of the registration of the inspection before use and adjustment two hot spots, system uses simpler, in hgher efficiency.
Accompanying drawing explanation
Fig. 1 is the structural representation of exciting light recording equipment in this utility model.
Fig. 2 is the structural representation consuming optical recorder in this utility model.
Fig. 3 is structural representation of the present utility model.
Fig. 4 is to excite hot spot and hollow type to consume the schematic diagram that Bright folding forms the fluorescence hot spot of compression.
Detailed description of the invention
For making the purpose of this utility model, technical scheme and advantage clearer, clear and definite, develop simultaneously enforcement referring to the drawings
This utility model is further described by example.
Refering to Fig. 1 to Fig. 3, this utility model provides the micro-system of STED super-resolution of a kind of volume holographic reduction scanning light beam
System, wherein, including exciting light recording equipment 1, launches exciting light and exciting light interference information is recorded on volume holographic material；Disappear
Deplete recording equipment 2, launch and consume light and consumption interference of light information is recorded on volume holographic material；Scanning light beam reduction apparatus
3, launch exciting light and consume light by the exciting light being recorded on volume holographic material and the optical information reduction consuming light；Polarization components
4, the exciting light being reduced and consumption light become line polarized light through polarization components 4；First light beam output precision 5, for transmission
Or reflection light beam；Two-dimensional scanning mirrors device 7, makes described exciting light and consumes light swing, it is achieved the two-dimensional scan to sample；Aobvious
Speck mirror 200, focused activating light and consumption light；Detection light path 300, including a photodetector 301, the sample returned from sample
The optical signal of product light is converted into the signal of telecommunication；Display and processing unit 400, be used for showing sample image and controlling described two-dimensional scan
Galvanometer device 7 is to realize the two-dimensional scan to sample.
Further, exciting light recording equipment 1 includes excitation light emission device the 10, second light beam output precision 11, coupling systems
Part 12, single-mode fiber 13, optical fiber collimator 14, beam splitting assembly 15, reflection subassembly 16 and volume holographic data board 17, at the present embodiment
In, excitation light emission device 10 is blue laser, and excitation light emission device 10 sends blue light excitation beam, and excitation beam is through described
Transmit in being coupled into described single-mode fiber 13 by described coupling assembly 12 after second light beam output precision 11 transmission, single-mode fiber 13
The excitation beam of exit end through optical fiber collimator 14 collimate after by the described partially reflective fractional transmission of beam splitting assembly 15, beam splitting group
Part 15 is semi-transparent semi-reflecting lens, to light beam one half transmitting half reflect, reflection light beam by after reflection subassembly 16 secondary reflection again with transmission
Light beam produces interferes, and the interference information of formation is recorded on described volume holographic data board 17.
Further, described consumption optical recorder 2 includes consuming optical transmitting set the 20, second light beam output precision 11, coupling
Seaming element 12, single-mode fiber 13, optical fiber collimator 14, beam splitting assembly 15, reflection subassembly 16,0 to 2 π vortex phase plate 21 and body
Holographic recording plate 17, in the present embodiment, consuming optical transmitting set 20 is red laser, and consumption optical transmitting set 20 sends HONGGUANG and disappears
Deplete bundle, consume light beam and be coupled into described single mode by described coupling assembly 12 after described second light beam output precision 11 reflection
Transmission in optical fiber 13, the consumption light beam of single-mode fiber 13 exit end after optical fiber collimator 14 collimates by beam splitting assembly 15 part
Reflecting part transmission, transmitted light beam forms phase-modulation light beam through 0 to 2 π vortex phase plate 21, and reflection light beam is by reflection subassembly
16 produce with transmitted light beam after secondary reflection and interfere, and the interference information of formation is recorded on described volume holographic data board 17, now
Volume holographic data board 17 have recorded the interference information of excitation beam and consumes the dry of light through what 0 to 2 π vortex phase plate 21 was modulated
Relate to information.
Excitation beam and to consume light beam be all to collimate from single-mode fiber 13 outgoing and by optical fiber collimator 14, it is believed that
Being the light beam that sends of the point source of two position consistency, two light beams can keep highly overlapping in recording process.
Further, described scanning light beam reduction apparatus 3 includes excitation light emission device 10, consumes optical transmitting set 20, second
Light beam output precision 11, coupling assembly 12, single-mode fiber 13, optical fiber collimator 14 and volume holographic data board 17, excitation light emission
Device 10 and luminescence while of consuming optical transmitting set 20, excitation beam and consumption light beam quilt after described second light beam output precision 11
Merging, the light beam composition after merging goes back elementary beam, transmits, single mode in being coupled into described single-mode fiber 13 by described coupling assembly 12
The light beam of optical fiber 13 exit end irradiates described volume holographic data board 17 after optical fiber collimator 14 collimates, and goes back elementary beam incidence side
To contrary with the light beam incident direction in exciting light recording equipment 1, consumption optical recorder 2.Volume holographic technology has wavelength choosing
Selecting property, the reduction light of incident specific wavelength can only reduce the light of corresponding wavelength, exciting light and consume light and restore collimation respectively
Exciting light and the consumption light modulated through 0 to 2 π vortex phase plate 21, two light beams of reduction can keep the coincidence of height.
Further, described two-dimensional scanning mirrors device 7 includes X-axis scanning galvanometer 71, Y axis scanning galvanometer 72 and a galvanometer
Controller 73, described galvanometer controller 73 controls described X-axis scanning galvanometer 71 and swings around X-direction, controls Y axis scanning galvanometer 72
Swing to realize the two-dimensional scan to sample around Y direction.Galvanometer controller 73 connects described display and processing unit 400, aobvious
Show that controlling galvanometer controller 73 with processing unit 400 drives X-axis scanning galvanometer 71 and Y axis scanning galvanometer 72 to excite by rule swing
Light beam and consumption light beam.In this example it is shown that be computer with processing unit 400.
In the present embodiment, also including relaying light path 8, described relaying light path 8 includes the first lens 81 and the second lens 82,
The rear focus of described first lens 81 overlaps with the object focus of described second lens 82, in X-axis scanning galvanometer 71 reflecting surface
Point overlaps with the object focus of the first lens 81, and the ratio of the first lens 2-13 and the second lens 2-14 focal length is:
F2/f1=D/d
Wherein, f1 is the first lens 81 focal length, and f2 is the second lens 82 focal length, and D is microcobjective 200 Entry pupil diameters, and d is
Exciting light and consumption light beam diameter, in order to make full use of the resolution capability of microcobjective 200, incident beam should be the most full
Microcobjective whole entrance pupil portion, needs to be amplified exciting light and consumption light light beam, arranges and include the first lens 81 and second
The relaying light path 8 of lens 82, makes f2/f1=D/d, can effectively amplify exciting light and consume the diameter of light light beam so that it is be full of aobvious
Speck mirror whole entrance pupil portion is to make full use of the resolution capability of microcobjective 200.
Further, it is additionally provided with colour killing between described first light beam output precision 5 and described two-dimensional scanning mirrors device 7
Differ from 1/2nd wave plates 6, between described second lens 82 and described microcobjective 200, be provided with achromatic quarter waveplate 9,
Exciting light and consumption light is made to be converted into preferably by adjusting achromatism 1/2nd wave plate 6 and achromatic quarter waveplate 9
Rotatory polarization, excites hot spot fluorescent, and hollow type consumption hot spot is by stimulated radiation consumption hot spot edge fluorescence, as shown in Figure 4,
Fluorescence hot spot is compressed, and makes spot size break through diffraction limit, it is achieved super-resolution.
In the present embodiment, described first light beam output precision 5 is fluorescence dichroic mirror, fluorescence dichroic mirror transmission blue light
And HONGGUANG, reflect and go out, from sample, the fluorescence returned.
In the present embodiment, described second light beam output precision 11 is dichroic mirror, transmission blue light, reflects HONGGUANG.
Further, described detection light path 300 also includes the optical filter 303 only allowing return sample light pass through and will return sample
Product optical coupling enters the 3rd lens 302 of multimode fibre, returns after sample light is coupled into multimode fibre and is transferred to described photoelectricity
Detector 301, photodetector 301 connects described display and processing unit 400, and display and processing unit 400 show sample in real time
Product image.
The STED super-resolution microscopic method step of this utility model volume holographic reduction scanning light beam is as follows:
Step A00: record exciting light, excitation light emission device 10 sends excitation beam, and excitation beam is defeated through the second light beam
Transmit in being coupled into single-mode fiber 13 by described coupling assembly 12 after going out assembly 11 transmission, the exciting light of single-mode fiber 13 exit end
Restrainting by the described partially reflective fractional transmission of beam splitting assembly 15 after optical fiber collimator 14 collimates, reflection light beam is by reflection subassembly 16
Producing with transmitted light beam after secondary reflection and interfere, the interference information of formation is recorded on described volume holographic data board；
Step B00: record consumes light, consumes optical transmitting set 20 and sends consumption light beam, consume light beam defeated through the second light beam
It is coupled assembly 12 after going out assembly 11 reflection and is coupled into transmission in single-mode fiber 13, the consumption light beam warp of single-mode fiber 13 exit end
Crossing after optical fiber collimator 14 collimates by the partially reflective fractional transmission of beam splitting assembly 15, transmitted light beam is through 0 to 2 π vortex phase plate
21 form phase-modulation light beam, and reflection light beam is produced with transmitted light beam after reflection subassembly again secondary reflection interferes, the interference of formation
Information is recorded on described volume holographic data board 17；
Step C00: scanning light beam reduces, excitation light emission device 10 and consumption optical transmitting set 20 are the most luminous, excitation beam
With consume light beam after described second light beam output precision 11 merged, the light beam after merging is coupled by described coupling assembly 12
Entering transmission in described single-mode fiber 13, the light beam of single-mode fiber 13 exit end irradiates record after optical fiber collimator 14 collimates to be had
Exciting light and the volume holographic data board 17 of consumption optical information；
Step D00: volume holographic data board 17 can restore the exciting light of the collimation being previously recorded and through 0 to 2 π whirlpool
The consumption light of rotation phase-plate modulation, the exciting light of the collimation being reduced and the consumption light warp through 0 to 2 π vortex phase plate modulation
After crossing polarization components 4, the first light beam output precision 5 is entered in transmission, after being reflected by X-axis scanning galvanometer 71 and Y axis scanning galvanometer 72 through
Relaying light path 8 is amplified, and excites hot spot and hollow type to disappear by being formed after microcobjective 200 after achromatic quarter waveplate 9
Depleting speckle, hollow type consumes hot spot and consumes hot spot edge fluorescence by stimulated radiation, forms less fluorescence hot spot；
Step E00: after the fluorescence signal of sample reflection is collected by microcobjective 200, return by original optical path, pass sequentially through and disappear
Aberration quarter-wave plate the 9, second lens the 82, first lens 81, Y axis scanning galvanometer 72, X-axis scanning galvanometer 71 and achromatism two
After/mono-wave plate 6, it is reflected into detecting light path 300 through the first light beam output precision 5, after fluorescence signal passes through optical filter 303,
Focusing on and be coupled to through the 3rd lens 302 in multimode fibre, fluorescence is from multimode fibre exit end outgoing, and by photodetector
301 detections, photodetector 301 converts optical signals into transmission extremely display and processing unit 400 after the signal of telecommunication.
This utility model, by providing the STED super-resolution microscopic system of a kind of volume holographic reduction scanning light beam, utilizes body complete
Breath technology, the consumption light beam recording after the excitation beam that height overlaps and 0～2 π phase-modulations on volume holographic material, profit
During with reference light reversely incident volume holographic material, can two light beam reduction, two beam heights being reduced be overlapped, height overlaps
Two light beams by stimulated radiation principle, consume and excite hot spot edge fluorescence, the size of compression fluorescence hot spot, it is achieved super-resolution shows
Micro-, solve the beam drift brought due to extraneous vibration, temperature difference well and cause the problem that registration is low, eliminate use
The step of the registration of front inspection and adjustment two hot spots, system uses simpler, in hgher efficiency.
It should be appreciated that application of the present utility model is not limited to above-mentioned citing, those of ordinary skill in the art are come
Saying, can be improved according to the above description or convert, all these modifications and variations all should belong to the appended power of this utility model
The protection domain that profit requires.
Claims (9)
1. the STED super-resolution microscopic system of a volume holographic reduction scanning light beam, it is characterised in that include
Exciting light recording equipment, launches exciting light and exciting light interference information is recorded on volume holographic material；
Consume optical recorder, launch and consume light and consumption interference of light information is recorded on volume holographic material；
Scanning light beam reduction apparatus, launches exciting light and consumes light by the exciting light being recorded on volume holographic material and consumption light
Optical information reduces；
Polarization components, the exciting light being reduced and consumption light become line polarized light through polarization components；
First light beam output precision, for transmission or reflection light beam；
Two-dimensional scanning mirrors device, makes described exciting light and consumes light swing, it is achieved the two-dimensional scan to sample；
Microcobjective, focuses on exciting light and consumption light on sample；
Detection light path, including a photodetector, is converted into the signal of telecommunication the optical signal of the sample light returned from sample；
Display and processing unit, for showing sample image and controlling described two-dimensional scanning mirrors device to realize two to sample
Dimension scanning.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that institute
State exciting light recording equipment and include excitation light emission device, the second light beam output precision, coupling assembly, single-mode fiber, fiber optic collimator
Device, beam splitting assembly, reflection subassembly and volume holographic data board, described excitation light emission device sends excitation beam, and excitation beam passes through
Transmit in being coupled into described single-mode fiber by described coupling assembly after described second light beam output precision transmission, single-mode fiber outgoing
The excitation beam of end is by described beam splitting components reflecting part transmission after optical fiber collimator collimates, and reflection light beam is reflected
Producing with transmitted light beam after assembly secondary reflection again and interfere, the interference information of formation is recorded on described volume holographic data board.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that institute
State consumption optical recorder to include consuming optical transmitting set, the second light beam output precision, coupling assembly, single-mode fiber, fiber optic collimator
Device, beam splitting assembly, reflection subassembly, 0 to 2 π vortex phase plate and volume holographic data board, described consumption optical transmitting set sends consumption light
Bundle, consumes in light beam is coupled into described single-mode fiber after described second light beam output precision reflection by described coupling assembly and passes
Defeated, the consumption light beam of single-mode fiber exit end through optical fiber collimator collimate after by the reflecting part transmission of beam splitting components, thoroughly
Irradiating light beam through 0 to 2 π vortex phase plate formed phase-modulation light beam, reflection light beam by after reflection subassembly again secondary reflection with transmission
Light beam produces interferes, and the interference information of formation is recorded on described volume holographic data board.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that institute
State scanning light beam reduction apparatus to include excitation light emission device, consume optical transmitting set, the second light beam output precision, coupling assembly, list
Mode fiber, optical fiber collimator and volume holographic data board, excitation light emission device and to consume optical transmitting set simultaneously luminous, excitation beam and
Consuming light beam merged after described second light beam output precision, the light beam after merging is coupled into described by described coupling assembly
Transmission in single-mode fiber, the light beam of single-mode fiber exit end irradiates described volume holographic data board after optical fiber collimator collimates.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that institute
State two-dimensional scanning mirrors device and include X-axis scanning galvanometer, Y axis scanning galvanometer and a galvanometer controller, described galvanometer controller control
Make described X-axis scanning galvanometer to swing around X-direction, control Y axis scanning galvanometer and swing to realize the two dimension to sample around Y direction
Scanning.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that also
Including relaying light path, described relaying light path includes the first lens and the second lens, and the rear focus of described first lens is with described
The object focus of the second lens overlaps.
The STED super-resolution microscopic system of volume holographic the most according to claim 6 reduction scanning light beam, it is characterised in that institute
State and between the first light beam output precision and described two-dimensional scanning mirrors device, be additionally provided with achromatism 1/2nd wave plate, described
It is provided with achromatic quarter waveplate between two lens and described microcobjective.
The STED super-resolution microscopic system of volume holographic the most according to claim 4 reduction scanning light beam, it is characterised in that institute
Stating the first light beam output precision is fluorescence dichroic mirror, and described second light beam output precision is dichroic mirror.
The STED super-resolution microscopic system of volume holographic the most according to claim 1 reduction scanning light beam, it is characterised in that institute
State detection light path also include the optical filter only allowing return sample light pass through and enter the 3rd of multimode fibre by returning sample optical coupling
Lens, return after sample light is coupled into multimode fibre and are transferred to described photodetector.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929526A (en) * | 2016-06-27 | 2016-09-07 | 佛山市南海区欧谱曼迪科技有限责任公司 | A STED super-resolution microscopic system and microscopic method for volume holographic reduction of scanning beam |
| CN115855252A (en) * | 2022-07-14 | 2023-03-28 | 华中科技大学 | Single photon sensitivity ultrafast spectral measurement and spectral imaging device and method |
-
2016
- 2016-06-27 CN CN201620666673.5U patent/CN205750086U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105929526A (en) * | 2016-06-27 | 2016-09-07 | 佛山市南海区欧谱曼迪科技有限责任公司 | A STED super-resolution microscopic system and microscopic method for volume holographic reduction of scanning beam |
| CN115855252A (en) * | 2022-07-14 | 2023-03-28 | 华中科技大学 | Single photon sensitivity ultrafast spectral measurement and spectral imaging device and method |
| CN115855252B (en) * | 2022-07-14 | 2024-04-09 | 华中科技大学 | Single photon sensitivity ultrafast spectrum measurement and spectrum imaging device and method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 528251 Guangdong Province Nanhai District of Foshan City, Guicheng Guicheng Ping Chau Street No. 1 Yongan Road, A block 504 Jingu Photoelectric Industrial community Patentee after: Guangdong euro Mandy Technology Co., Ltd. Address before: 528251 Guangdong Province Nanhai District of Foshan City, Guicheng Guicheng Ping Chau Street No. 1 Yongan Road, A block 504 Jingu Photoelectric Industrial community Patentee before: FOSHAN CITY NANHAI DISTRICT OPTOMEDIC SCIENCE & TECHNOLOGY CO., LTD. |
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20161130 Effective date of abandoning: 20180821 |