CN205322304U - Obtain device of clear microcirculation image - Google Patents
Obtain device of clear microcirculation image Download PDFInfo
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- CN205322304U CN205322304U CN201620067606.1U CN201620067606U CN205322304U CN 205322304 U CN205322304 U CN 205322304U CN 201620067606 U CN201620067606 U CN 201620067606U CN 205322304 U CN205322304 U CN 205322304U
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Abstract
The utility model discloses an obtain device of clear microcirculation image, include: microscope optical system, at least one small light source projection unit to and, formation of image capture device, microscope optical system leads to the light zone territory including the formation of image, small light source projection unit evenly distributed is in the formation of image is led to around the light zone territory, small light source projection unit's light emission direction and perpendicular to is examined the direction formation projectional angle of thing to, the projectional angle is greater than arcsin (NA), wherein, parameter NA is microscope optical system's numerical aperture, arcsin is arcsine operator, mirror optical system with the image formation by rays that obtains throw to formation of image capture device obtains image data. Implement the utility model provides an embodiment can not received reflection light influences's picture rich in detail on need not to use the basis of polarized light, design benefit is with low costs.
Description
Technical field
This utility model relates to optical image technology field, particularly relates to a kind of microcirculation picture rich in detail catching method and device。
Background technology
In blood circulation of human body system, microcirculation refers to the blood circulation between arteriole and venule, is carry oxygen and nutrient substance to histiocyte and to transport carbon dioxide CO2With the final link of metabolite, be also most important link。Microcirculatory perfusion is extremely important for cellular metabolism, and microcirculatory perfusion obstacle will cause serious dysbolismus, and serious conditions will cause the exhaustion of each histoorgan and causes death。In particular how monitoring microcirculation situation, quickly and easily monitor the microcirculation situation of critical patient in real time, and such as early discovery shock (microcirculation failure) sign etc., the survival rate for improving critical patient is extremely crucial。
At present on visual microcirculation in human body is monitored, its monitoring device mainly improves from aspects such as lighting system, three-dimensional imaging principles, thus improving picture contrast or obtaining stereo-picture；But device is monitored for the observation of internal (such as the sublingual mucosa etc.) microcirculatory perfusion in little space and the area-of-interest observed in visual field carries out finer observation then rarely have and relate to for microcirculation。
It is conventionally used to the product observing little space, is usually the axis light illumination adopting illumination path and imaging optical path to share the same space。At this moment, illuminating ray is almost that perpendicular projection is in skin tissue surface。It is generally skin or mucosa due to observed object, when having uneven oil membrane or horny layer when skin or mucous membrane surface, then can form mirror effect, produce strong reflection light。If reflection light enters imaging system, owing to reflecting signal intensity the diffusing much larger than scattering of light, diffusing of the skin histology scattering needed for normal observation is just submerged, and image quality is also just unintelligible。
Reflective in order to avoid skin surface, traditional method can increase the polarization of light polarizer in illumination path, and making illumination light is polarized light；Meanwhile, in imaging optical path, add cross-polarization sheet eliminate the polarized light of skin surface reflection in imaging optical path。But, the oil membrane of skin or mucous membrane surface or horny layer are uneven, so reflection light is uneven polarization light。Reflection light is by after imaging optical path cross-polarization sheet, and image can form the shade of sheet, still affects the definition of image。
Utility model content
Technical problem to be solved in the utility model is, defect in order to avoid above-mentioned prior art, a kind of device obtaining clear Microcirculatory Image is provided, re-construct light path, on the basis without using polarized light, the reflection light making checking matter surface cannot be introduced into imaging optical path, thus obtaining the picture rich in detail not affected by reflection light。
For solving above technical problem, this utility model embodiment provides a kind of device obtaining clear Microcirculatory Image, including: microscope optical system, at least one small light source projection device, and, imaging acquisition equipment；Described microscope optical system includes territory, imaging transparent zone；Described small light source projection device is evenly distributed on around territory, described imaging transparent zone；
The light of described small light source projection device launches direction and the direction being perpendicular to checking matter forms projectional angle, and, projectional angle is more than arcsin (NA)；Wherein, parameter NA is the numerical aperture of microscope optical system；Arcsin is arcsine operative symbol；The image formation by rays of acquisition is projected to described imaging acquisition equipment by described microscope optical system, it is thus achieved that view data。
Further, it is thus achieved that the device of clear Microcirculatory Image, area of computer aided imaging device is also included；Described area of computer aided imaging device and the communication connection of described imaging acquisition equipment, after carrying out digital processing to described view data, it is shown that microcirculation digital picture。
Preferably, described microscope optical system includes aperture diaphragm and multiple eyeglass for image formation by rays, to adjust the optical field of view of described microscope optical system, systemic resolution and amplification。
Preferably, described small light source projection device includes small LED lamp bead array；Described small LED lamp bead array includes the multiple LED being evenly arranged around territory, described imaging transparent zone。
Preferably, territory, described imaging transparent zone is annular, and aperture diaphragm and multiple eyeglass for image formation by rays in described microscope optical system are arranged in territory, transparent zone。
Further, described multiple LED are fixed on substrate；Described substrate is provided with the wire providing power supply to each LED。
The device obtaining clear Microcirculatory Image that this utility model embodiment provides, utilize the feature of optical field, light propagation characteristic in conjunction with measured object (especially the interior tissue of skin or mucosa), by arranging the projectional angle size of the small light source projection device relative position with measured object and control incident ray dexterously, the reflection light avoiding the illumination path on measured object surface enters microscope optical system, simultaneously, the imaging optical path light that the scattering properties of projectional angle size and measured object determines after measured object interior tissue diffuse-reflectance can enter microscope optical system, therefore, the diffuse-reflectance light of imaging optical path is not superimposed with the reflection light of illumination path, namely imaging optical path is not by the impact of reflection light, thus realizing without using on the basis that polarized light and polaroid filter, obtain Microcirculatory Image clearly, structure is ingenious, cost is low。
Accompanying drawing explanation
Fig. 1 is the structural representation of an embodiment of the device obtaining clear Microcirculatory Image that this utility model provides。
Fig. 2 is the upward view of a kind of structure of the small light source projection device that this utility model provides。
Fig. 3 is the microscope optical system light distribution schematic diagram with small light source projection device and measured object of this utility model offer。
Fig. 4 is the light path geometric angle schematic diagram of the device obtaining clear Microcirculatory Image that this utility model provides。
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is clearly and completely described。
Referring to Fig. 1, it it is the structural representation of an embodiment of the device obtaining clear Microcirculatory Image that this utility model provides。
In the present embodiment, the described device obtaining clear Microcirculatory Image, including: microscope optical system 10, at least one small light source projection device 20, and, imaging acquisition equipment 30；Described microscope optical system 10 includes territory, imaging transparent zone 11；Described small light source projection device 20 is evenly distributed on around territory, described imaging transparent zone 11。
Referring to Fig. 2, it it is the upward view of a kind of structure of the small light source projection device that this utility model provides。
The light of described small light source projection device 20 launches direction and the direction being perpendicular to checking matter forms projectional angle ρ, and, projectional angle ρ is more than arcsin (NA)；Wherein, parameter NA is the numerical aperture of microscope optical system；Arcsin is arcsine operative symbol；
The image formation by rays of acquisition is projected to described imaging acquisition equipment 30 by described microscope optical system 10；Described imaging acquisition equipment 30 is for processing described image formation by rays, it is thus achieved that view data。
The present embodiment, without using polarized light and polaroid to filter, by controlling illuminating ray projection angle, can avoid skin tissue surface specular light B to enter microscope optical system 10, only allows the skin histology C that diffuses to enter microscope optical system 10。The light of microcirculation object reflection enters microscope optical system, arrives imaging acquisition equipment, obtains clearly Microcirculatory Image by area of computer aided image-forming module。
Further, as it is shown in figure 1, the device obtaining clear Microcirculatory Image that the present embodiment provides also includes area of computer aided imaging device 40。
Described area of computer aided imaging device 40 communicates to connect with described imaging acquisition equipment 30, after described view data is carried out digital processing, it is shown that microcirculation digital picture。
For microscope optical system 10, numerical aperture (NA) is the important parameter of optics。Such as, the series microscope object lens of GB2609-81 standard, microscope all can indicate the parameter of its numerical aperture (NA)。
Referring to table 1, it is multiple examples of the basic parameter of the microscope optical system object lens that this utility model provides。
The basic parameter of the microscope optical system object lens that table 1 this utility model provides
Numerical aperture NA is the important parameter of microscope objective, and with the amplification of object lens, operating distance, the depth of field has direct relation。In general, it is directly proportional to resolution, determines the size of its resolving power。
Referring to Fig. 3, it it is the microscope optical system light distribution schematic diagram with small light source projection device and measured object of this utility model offer。
Numerical aperture NA is the product of the sine of the refractive index (n) of medium between object lens frontal lens and tested object and angular aperture θ half, it is possible to be formulated as follows:
NA=n*sin (θ/2) (1)
Angular aperture also known as " mirror bicker ", the angle that to be object point in objective lens optical axis formed with the effective diameter of object lens frontal lens。Only the light within the scope of angular aperture θ could enter microscope optical system 10, and the extraneous light of angular aperture θ cannot be introduced into microscope optical system 10, does not participate in imaging, thus does not affect image quality。
When measured object is skin histology, the horny layer of skin tissue surface is similar to minute surface, in accordance with the reflection law of light, can reflect light consumingly。It it is good light-scattering body under skin histology 100 micrometer depth, in accordance with scattering of light law, can scattering light effectively, scattered beam C ' is produced inside skin histology, after scattered beam C ' appears skin histology, produce in the diffuse-reflectance light C image formation by rays device entering microscope optical system 10 at skin surface。
Referring to Fig. 4, it it is the light path geometric angle schematic diagram of the device obtaining clear Microcirculatory Image that this utility model provides。
Small light source projection device 10 emits beam A with the projectional angle ρ with vertical direction angle, arrives skin tissue surface；Owing to the horny layer of skin tissue surface is similar to minute surface, in accordance with the reflection law of light, as shown in Figure 4, then there is following relation:
Incident angle α=projectional angle ρ (2)
Angle of reflection β=incident angle α (3)
To avoid reflection light B to enter microscope optical system 10, figure 4, it is seen that necessarily require angle of reflection β and angular aperture θ to meet relation: β > θ/2；Therefore, it can release the projectional angle ρ launching light A and must be fulfilled for following condition: ρ > θ/2；Meanwhile, the relation according to equation (1) known numeric value aperture NA and refractive index n, angular aperture θ, and the refractive index n=1.0 of air, then can draw there is following relation in atmosphere:
Angular aperture (θ)=2*arcsin (NA) (4)
Then as projectional angle ρ > arcsin (NA), can avoid the reflection light B of skin surface to enter stacking image in microscope optical system 10。
Such as, for the microscope that resolution is higher, generally its numerical aperture NA=0.25, then according to above-mentioned derivation it can be seen that projectional angle ρ needs more than 14.5 degree。Owing under skin histology 100 micrometer depth being good light-scattering body, in accordance with scattering of light law, scattered beam C ' reflects skin histology internal feature, enters in microscope optical system 10 and carry out imaging after appearing skin surface。
Preferably, the present embodiment is used for observing biology (especially human body) inner skin tissues microcirculation situation。
When being embodied as, described microscope optical system 10 includes aperture diaphragm and multiple eyeglass for image formation by rays, to adjust the optical field of view of described microscope optical system, systemic resolution and amplification。
In the present embodiment, as in figure 2 it is shown, described small light source projection device 20 includes small LED lamp bead array；Described small LED lamp bead array includes the multiple LED (illustrate 4 compact LEDs in Fig. 2, but be not limited to 4) being evenly arranged around territory, described imaging transparent zone 11。Small light source projection device 20, for light is projected to measured object surface, forms the region of a uniform-illumination on described measured object surface。
Preferably, territory, described imaging transparent zone 11 is annular, and aperture diaphragm and multiple eyeglass for image formation by rays in described microscope optical system 10 are arranged in territory, transparent zone 11。The internal diffuse-reflectance light C returned of skin histology, after several eyeglasses of microscope optical system 10, becomes the picture of the handstand amplified on imaging acquisition equipment 30 surface。In specific implementation process, microscope optical system 10 at least includes an aperture diaphragm and two eyeglasses so that its optical field of view, systemic resolution, and amplification all can meet the observation of blood cell in microcirculation。Specifically, described multiple LED are fixing on the base plate (21；Described substrate 21 is provided with the wire (not shown in Fig. 2) to each LED offer power supply。
The device obtaining clear Microcirculatory Image that this utility model embodiment provides, utilize rule and the feature of optics aspect, light propagating characteristic in conjunction with measured object (especially the interior tissue of skin or mucosa), by arranging the projectional angle size of the small light source projection device relative position with measured object and control incident ray dexterously, reconstruct light path, the reflection light avoiding the illumination path on measured object surface enters microscope optical system, simultaneously, the imaging optical path light determined after measured object interior tissue diffuse-reflectance due to the scattering properties of projectional angle size and measured object can enter microscope optical system, therefore, the diffuse-reflectance light of imaging optical path is not superimposed with the reflection light of illumination path, namely imaging optical path is not by the impact of reflection light, thus realizing without using on the basis that polarized light and polaroid filter, obtain Microcirculatory Image clearly, design ingenious, cost is low。
The above is preferred implementation of the present utility model; it should be pointed out that, for those skilled in the art, under the premise without departing from this utility model principle; can also making some improvements and modifications, these improvements and modifications are also considered as protection domain of the present utility model。
Claims (6)
1. the device obtaining clear Microcirculatory Image, it is characterised in that including: microscope optical system, at least one small light source projection device, and, imaging acquisition equipment；Described microscope optical system includes territory, imaging transparent zone；Described small light source projection device is evenly distributed on around territory, described imaging transparent zone；
The light of described small light source projection device launches direction and the direction being perpendicular to checking matter forms projectional angle, and, projectional angle is more than arcsin (NA)；Wherein, parameter NA is the numerical aperture of microscope optical system；Arcsin is arcsine operative symbol；The image formation by rays of acquisition is projected to described imaging acquisition equipment by described microscope optical system, it is thus achieved that view data。
2. the device obtaining clear Microcirculatory Image as claimed in claim 1, it is characterised in that described device also includes area of computer aided imaging device；Described area of computer aided imaging device and the communication connection of described imaging acquisition equipment。
3. the device obtaining clear Microcirculatory Image as claimed in claim 1, it is characterised in that described microscope optical system includes aperture diaphragm and multiple eyeglass for image formation by rays。
4. the device obtaining clear Microcirculatory Image as claimed in claim 3, it is characterised in that described small light source projection device includes small LED lamp bead array；Described small LED lamp bead array includes the multiple LED being evenly arranged around territory, described imaging transparent zone。
5. the device obtaining clear Microcirculatory Image as claimed in claim 4, it is characterised in that territory, described imaging transparent zone is annular, and aperture diaphragm and multiple eyeglass for image formation by rays in described microscope optical system are arranged in territory, transparent zone。
6. the device obtaining clear Microcirculatory Image as claimed in claim 5, it is characterised in that described multiple LED are fixed on substrate；Described substrate is provided with the wire providing power supply to each LED。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620067606.1U CN205322304U (en) | 2016-01-22 | 2016-01-22 | Obtain device of clear microcirculation image |
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| CN201620067606.1U CN205322304U (en) | 2016-01-22 | 2016-01-22 | Obtain device of clear microcirculation image |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725345A (en) * | 2017-01-24 | 2017-05-31 | 青岛大学 | The device of human microvascular ultra microstructure is detected using circular polarization effluent dark field imaging technique |
| CN110192840A (en) * | 2018-02-27 | 2019-09-03 | 皇家飞利浦有限公司 | Obtain the image for determining one or more characteristics of the skin of object |
-
2016
- 2016-01-22 CN CN201620067606.1U patent/CN205322304U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106725345A (en) * | 2017-01-24 | 2017-05-31 | 青岛大学 | The device of human microvascular ultra microstructure is detected using circular polarization effluent dark field imaging technique |
| CN106725345B (en) * | 2017-01-24 | 2023-08-11 | 青岛大学 | Device for detecting human microvascular ultrastructure by using circular polarization sidestream dark field imaging technology |
| CN110192840A (en) * | 2018-02-27 | 2019-09-03 | 皇家飞利浦有限公司 | Obtain the image for determining one or more characteristics of the skin of object |
| CN110192840B (en) * | 2018-02-27 | 2023-12-22 | 皇家飞利浦有限公司 | Obtaining an image for determining one or more characteristics of the skin of a subject |
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