CN204269547U - A kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image - Google Patents
A kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image Download PDFInfo
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- CN204269547U CN204269547U CN201420712258.XU CN201420712258U CN204269547U CN 204269547 U CN204269547 U CN 204269547U CN 201420712258 U CN201420712258 U CN 201420712258U CN 204269547 U CN204269547 U CN 204269547U
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Abstract
The utility model discloses a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, mainly comprise: laser instrument, it launches short-pulse laser signal; Lighting source, it launches visible light signal; Refraction reflected light path, wherein wrapped multiple Transflective mirror, propagated all straight down with the light signal making described laser instrument and lighting source send; Object lens, it is positioned at the below of described refraction reflected light path, and the light signal propagated straight down, through described object lens, is radiated in the examined object below object lens; Travel mechanism, it moves described light signal or mobile described examined object, makes described light signal travel through all parts of described examined object.LIBS analytical technology and laser scanning co-focusing technology organically combine by the utility model, adopt the point by point scanning of spectrum micro-imaging technique realization to testing sample, achieve a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image.
Description
Technical field
The utility model relates to a kind of microoptic imaging technique, particularly a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image.
Background technology
High-resolution microoptic imaging technique is that people turn to microcosmic from macroscopic view, formation and the regulatory mechanism of life is dynamically disclosed in real time at cellular and molecular level, obtain a series of original scientific discovery, realize the important means of comprehensive mixing together of life science and the subject such as physics, chemistry.From the supermicroscope till now of conventional microscopy the earliest, as super-resolution microscope, Two Photon Fluorescence and scanning tunnelling microscope etc., can say that the appearance of each new imaging technique all has landmark meaning.Along with the appearance of laser technology, light-matter interaction is applied to micro-imaging and material analysis, not only can improve the spatial resolution of optical microscopy widely, more can realize the detection of measured object component and structure, can also three-dimensional imaging be carried out simultaneously.
As multiphoton fluorescence imaging, second harmonic and third harmonic imaging etc., all there is good application example in the nonlinear optics imaging technique of current report.Exceed photon fluorescence technology majority of case to need to inject fluorescent marker, and second harmonic and third harmonic imaging are also because it is to the particular/special requirement of measured object non-linear nature, range of application receives extreme influence.
Laser-induced Breakdown Spectroscopy (LIBS) is a kind of important novel analytical technology of generally acknowledging at present.This technology requires low, applied widely to the early-stage preparations of sample, damages the features such as little.But traditional LIBS needs the spectroscopic analysis system using relative complex, and amount of test data is large, and the processing time is long; And the sensitivity of its spectroscopic analysis system, once deficiency, needs to rely on the light intensity increasing exciting light to improve plasma signal intensity, while the damage of increase object under test, greatly reduces spatial resolution.Therefore, in micro-imaging, the application of LIBS has significant limitation.
Just because of the limitation of LIBS technology, the utility model is in conjunction with LIBS analytical technology and laser scanning co-focusing technology, achieve a kind of novel microscopical imaging spectrometer system of Elemental redistribution that not only can obtain appearance images but also can observe object under test, this system architecture is simple, convenient operation.
Utility model content
The utility model is for above-mentioned limitation, LIBS analytical technology and laser scanning co-focusing technology are organically combined, adopt the point by point scanning of spectrum micro-imaging technique realization to testing sample, achieve a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image.This technology is applicable to the every field such as bio-medical analysis, material analysis, soil analysis and atmospheric analysis.
An object of the present utility model is, provides a kind of Elemental redistribution scanning mirror be superimposed upon on laser scanning co-focusing microscope basis, to reduce costs to greatest extent.
Another object of the present utility model is, provides a kind of light path simple Elemental redistribution scanning mirror, while detecting the distribution of object interior element, can also show the surface image of object, the combination of both realizations.
For achieving the above object and some other object, the utility model is achieved through the following technical solutions:
Obtain a micro imaging system for appearance images and Elemental redistribution image simultaneously, comprising:
Laser instrument 1, it launches short-pulse laser signal;
Lighting source 12, it launches visible light signal;
Refraction reflected light path, comprising multiple Transflective mirror, propagates all straight down with the light signal making described laser instrument 1 and lighting source 12 send;
Object lens 5, it is positioned at the below of described refraction reflected light path, and the light signal propagated straight down, through described object lens 5, is radiated in the examined object below object lens;
Travel mechanism, it moves described light signal or mobile described examined object, makes described light signal travel through all parts of described examined object;
Wherein, examined object described in described short-pulse laser signal excitation produces plasma, and the visible light signal of described lighting source 12 passes back through object lens after described examined object reflection, and arrive image processor 8, obtain the appearance images of examined object; And the short-pulse laser signal of described laser instrument 1 passes back through object lens after described examined object reflection, and through echelle grating 14, generate spectrophotometric spectra, after being converged by concave mirror 6, arrive photodetector 15, it is by the Elemental redistribution image of the electromagnetic intensity determination examined object in detection spectrophotometric spectra.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, described refraction reflected light path comprises:
First semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, short-pulse laser signal incident described first semi-transparent semi-reflecting lens in the horizontal direction that described laser instrument 1 is launched, and get the short-pulse laser signal straight down of its reflection;
Second semi-transparent semi-reflecting lens, itself and horizontal direction are that 135 degree of angles are arranged, the short-pulse laser signal of the first semi-transparent semi-reflecting lens reflection continues to propagate straight down through the second semi-transparent semi-reflecting lens, and lighting source 12 from the side contrary with described laser instrument 1 incident described second semi-transparent semi-reflecting lens in the horizontal direction, get the visible light signal straight down of its reflection, the incidence point of lighting source 12 is identical with the breakthrough point of described short-pulse laser signal;
Thus, short-pulse laser signal and visible light signal all become the light signal propagated straight down.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, described refraction reflected light path also comprises:
3rd semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, the light signal propagated straight down continues to propagate downwards through the 3rd semi-transparent semi-reflecting lens, and the reflected light of visible light signal in examined object passes back through the 3rd semi-transparent semi-reflecting lens, get its reflected light, and the reflected light of short-pulse laser signal in examined object returns the 3rd semi-transparent semi-reflecting lens, get its transmitted light, the return light path of lighting source 12 and laser instrument 1 to be distinguished.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image,
Described travel mechanism is the movable stand along the movement of X, Y, Z three-dimensional, and it is arranged on the below of described examined object, drives described examined object to move, to realize traversal.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image,
Described travel mechanism is scanning galvanometer 17, and it is arranged between described 3rd semi-transparent semi-reflecting lens and object lens 5, by changing the angle of the light signal propagated straight down, to realize traversal.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Imaging len 9, it is arranged in the return light path of lighting source, for converging by the reflected light in the visible ray of the 3rd semi-transparent semi-reflecting lens;
Image processor 8, it is arranged on the focal length place of described imaging len 9, and the visible light signal of convergence is converted into electric signal, processes image.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Signal amplifier 16, it is connected, for amplification signal with described photodetector communication.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Computing machine 2, for the electric signal of Collection and analysis signal amplifier and image processor, carries out display and analysis chart picture.
Preferably, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Stepper motor 7, itself and described computing machine 2 connection, for controlling the rotation of described echelle grating 14.
Disclosed in the utility model, a kind of beneficial effect simultaneously obtaining the micro imaging system of appearance images and Elemental redistribution image at least comprises: LIBS analytical technology and laser scanning co-focusing technology are organically combined, adopt the point by point scanning of spectrum micro-imaging technique realization to testing sample, achieve a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image.
Accompanying drawing explanation
Fig. 1 is a kind of micro imaging system schematic diagram simultaneously obtaining appearance images and Elemental redistribution image described in the utility model one embodiment.
Embodiment
Below in conjunction with embodiment, the utility model is described in further detail, can implements according to this with reference to instructions word to make those skilled in the art.
As shown in Figure 1, the utility model discloses a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, namely can either show the outward appearance image of object, the distribution situation of various element in object can be reflected again.Such as iron sheet is detected, while its trickle image of displaying, also reflect in this iron sheet the content and distribution that comprise interior chromium element, carbon, iron oxide compound etc.Described micro imaging system comprises:
Laser instrument 1, it launches short-pulse laser signal.When short-pulse laser gets to a certain position of material to be analyzed, object can be excited to produce plasma in this position.And by plasma, light splitting is carried out to the reflected light that this short-pulse laser produces, after obtaining the spectrum of reflected light, material can be obtained by the elemental composition at scanning position place by analyzing spectrum again.Therefore, as long as allow short-pulse laser line by line scan all positions of material to be analyzed, just can obtain the elemental composition of all positions, position relationship and corresponding elemental composition are depicted as chart, just can the distribution diagram of element obtaining object under test.
Lighting source 12, it launches visible light signal, for irradiating object under test.Equally, a location point on lighting source once irradiating object under test, obtains the reflected light of this location point, utilizes reflected light to carry out imaging to this location point.Afterwards by mobile object under test itself or by the irradiating angle of portable lighting light source, make lighting source 12 travel through each location point on object under test, to the last obtain the image of all positions of object under test.Finally position relationship is become chart with corresponding Image Rendering, just can obtain its appearance images.
Refraction reflected light path, comprising multiple Transflective mirror, propagates all straight down with the light signal making described laser instrument 1 and lighting source 12 send, just in time normal incidence can enter object lens.In refraction radiation light path, those skilled in the art according to the rudimentary knowledge of light path, can arrange Transflective mirror, and the light signal that final described laser instrument 1 and lighting source 12 are sent is propagated all straight down.And preferably make described laser instrument 1 and the light signal that lighting source 12 sends along identical propagated, certain location point in such examined object obtains illumination and short-pulse laser simultaneously.
In a preferred embodiment of the present utility model, described refraction radiation light path can comprise:
First semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, short-pulse laser signal incident described first semi-transparent semi-reflecting lens in the horizontal direction that described laser instrument 1 is launched, and get the short-pulse laser signal straight down of its reflection;
Second semi-transparent semi-reflecting lens, itself and horizontal direction are that 135 degree of angles are arranged, the short-pulse laser signal of the first semi-transparent semi-reflecting lens reflection continues to propagate straight down through the second semi-transparent semi-reflecting lens, and lighting source 12 from the side contrary with described laser instrument 1 incident described second semi-transparent semi-reflecting lens in the horizontal direction, get the visible light signal straight down of its reflection, the incidence point of lighting source 12 is identical with the breakthrough point of described short-pulse laser signal;
Thus, short-pulse laser signal and visible light signal all become the light signal propagated straight down.
Described refraction reflected light path also comprises: the 3rd semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, the light signal propagated straight down continues to propagate downwards through the 3rd semi-transparent semi-reflecting lens, and the reflected light of visible light signal in examined object passes back through the 3rd semi-transparent semi-reflecting lens, get its reflected light, and the reflected light of short-pulse laser signal in examined object returns the 3rd semi-transparent semi-reflecting lens, get its transmitted light, the return light path of lighting source 12 and laser instrument 1 to be distinguished;
Object lens 5, it is positioned at the below of described refraction reflected light path, and the light signal propagated straight down, through described object lens 5, is radiated in the examined object below object lens;
Travel mechanism, it is changed the angle of the light signal propagated straight down by micro-scanning galvanometer 17 that moves or is moved movable stand to move described examined object along X, Y, Z three-dimensional.Also can adopt both, closely cooperating by both, makes described light signal travel through all parts of described examined object simultaneously;
Further, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Imaging len 9, it is arranged in the return light path of lighting source, for converging by the reflected light in the visible ray of the 3rd semi-transparent semi-reflecting lens.
Image processor 8, it is arranged on the focal length place of described imaging len 9, and the visible light signal of convergence is converted into electric signal, realizes the acquisition of image, storage, transmission, process and reproduction.
Further, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Signal amplifier 16, it is connected with described photodetector communication, for amplify described photodetector the electric signal that transforms out.
Further, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Computing machine 2, for the electric signal of Collection and analysis signal amplifier and image processor, and is depicted as its outward appearance imaging and its Elemental redistribution image according to electric signal.
Further, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, also comprises:
Stepper motor 7, itself and computing machine 2 connection, for controlling the rotation of echelle grating 14.
Further, a kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image, forms four light paths:
Article 1, light path: the illumination light sent by lighting source 12, enters object lens 5, focuses on examined object 3 after the second semi-transparent semi-reflecting lens 11 reflects;
Article 2, light path: the illumination light that examined object 3 reflects is propagated along the opposite direction of illumination light, after being collected by object lens 5 again, successively through the 3rd semi-transparent semi-reflecting lens 10, imaging len 9 and image processor 8, realize the appearance images of observing examined object by computing machine 2;
Article 3, light path: the laser pulse that short-pulse laser 1 exports enters object lens 5 after the reflection of the first semi-transparent semi-reflecting lens 13, is irradiated to examined object 3 after these object lens focus on;
Article 4, light path: the plasma resonance light that laser excitation examined object 3 produces is propagated along the direction that exciting light is contrary, collect through object lens 5, by the first semi-transparent semi-reflecting lens 13 and echelle grating 14, generate spectrophotometric spectra, converge to highly sensitive photodetector 15 by concave mirror 6, be converted into electric signal, then by signal amplifier 16 amplification signal, and be transferred to computing machine 2, thus observe the Elemental redistribution of examined object.
Although embodiment of the present utility model is open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable field of the present utility model completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the utility model is not limited to specific details and illustrates here and the legend described.
Claims (9)
1. obtain a micro imaging system for appearance images and Elemental redistribution image simultaneously, it is characterized in that, comprising:
Laser instrument (1), it launches short-pulse laser signal;
Lighting source (12), it launches visible light signal;
Refraction reflected light path, comprising multiple Transflective mirror, propagates all straight down with the light signal making described laser instrument (1) and lighting source (12) send;
Object lens (5), it is positioned at the below of described refraction reflected light path, and the light signal propagated straight down, through described object lens (5), is radiated in the examined object below object lens;
Travel mechanism, it moves described light signal or mobile described examined object, makes described light signal travel through all parts of described examined object;
Wherein, examined object described in described short-pulse laser signal excitation produces plasma, and the visible light signal of described lighting source (12) passes back through object lens after described examined object reflection, and arrive image processor (8), obtain the appearance images of examined object; And the short-pulse laser signal of described laser instrument (1) passes back through object lens after described examined object reflection, and through echelle grating (14), generate spectrophotometric spectra, after being converged by concave mirror (6), arrive photodetector (15), it is by the Elemental redistribution image of the electromagnetic intensity determination examined object in detection spectrophotometric spectra.
2. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 1, it is characterized in that, described refraction reflected light path comprises:
First semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, short-pulse laser signal incident described first semi-transparent semi-reflecting lens in the horizontal direction that described laser instrument (1) is launched, and get the short-pulse laser signal straight down of its reflection;
Second semi-transparent semi-reflecting lens, itself and horizontal direction are that 135 degree of angles are arranged, the short-pulse laser signal of the first semi-transparent semi-reflecting lens reflection continues to propagate straight down through the second semi-transparent semi-reflecting lens, and lighting source (12) from the side contrary with described laser instrument (1) incident described second semi-transparent semi-reflecting lens in the horizontal direction, get the visible light signal straight down of its reflection, the incidence point of lighting source (12) is identical with the breakthrough point of described short-pulse laser signal;
Thus, short-pulse laser signal and visible light signal all become the light signal propagated straight down.
3. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 2, it is characterized in that, described refraction reflected light path also comprises:
3rd semi-transparent semi-reflecting lens, itself and horizontal direction are that miter angle is arranged, the light signal propagated straight down continues to propagate downwards through the 3rd semi-transparent semi-reflecting lens, and the reflected light of visible light signal in examined object passes back through the 3rd semi-transparent semi-reflecting lens, get its reflected light, and the reflected light of short-pulse laser signal in examined object returns the 3rd semi-transparent semi-reflecting lens, get its transmitted light, the return light path of lighting source (12) and laser instrument (1) to be distinguished.
4. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 3, is characterized in that,
Described travel mechanism is the movable stand along the movement of X, Y, Z three-dimensional, and it is arranged on the below of described examined object, drives described examined object to move, to realize traversal.
5. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 4, is characterized in that,
Described travel mechanism is scanning galvanometer (17), and it is arranged between described 3rd semi-transparent semi-reflecting lens and object lens (5), by changing the angle of the light signal propagated straight down, to realize traversal.
6. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 5, is characterized in that, also comprise:
Imaging len (9), it is arranged in the return light path of lighting source, for converging by the reflected light in the visible ray of the 3rd semi-transparent semi-reflecting lens;
Image processor (8), it is arranged on the focal length place of described imaging len (9), and the visible light signal of convergence is converted into electric signal, processes image.
7. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 6, is characterized in that, also comprise:
Signal amplifier (16), it is connected, for amplification signal with described photodetector communication.
8. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 7, is characterized in that, also comprise:
Computing machine (2), for the electric signal of Collection and analysis signal amplifier and image processor, carries out display and analysis chart picture.
9. the micro imaging system simultaneously obtaining appearance images and Elemental redistribution image as claimed in claim 8, is characterized in that, also comprise:
Stepper motor (7), itself and described computing machine (2) connection, for controlling the rotation of described echelle grating (14).
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| CN201420712258.XU CN204269547U (en) | 2014-11-24 | 2014-11-24 | A kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image |
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| CN201420712258.XU CN204269547U (en) | 2014-11-24 | 2014-11-24 | A kind of micro imaging system simultaneously obtaining appearance images and Elemental redistribution image |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390943A (en) * | 2014-11-24 | 2015-03-04 | 中国科学院苏州生物医学工程技术研究所 | Microscopic imaging system capable of simultaneously obtaining appearance image and element distribution image |
| CN107049242A (en) * | 2017-01-24 | 2017-08-18 | 青岛大学 | Scan-type human microvascular ultra microstructure 3-D imaging system |
| CN107883895A (en) * | 2017-11-21 | 2018-04-06 | 苏州睿牛机器人技术有限公司 | Multiple light courcess laser beam detecting sensor and its detection method |
| CN115791756A (en) * | 2022-11-24 | 2023-03-14 | 北京杏林睿光科技有限公司 | Laser-induced breakdown spectroscopy device for measuring full spectrum at one time |
-
2014
- 2014-11-24 CN CN201420712258.XU patent/CN204269547U/en not_active Withdrawn - After Issue
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104390943A (en) * | 2014-11-24 | 2015-03-04 | 中国科学院苏州生物医学工程技术研究所 | Microscopic imaging system capable of simultaneously obtaining appearance image and element distribution image |
| CN107049242A (en) * | 2017-01-24 | 2017-08-18 | 青岛大学 | Scan-type human microvascular ultra microstructure 3-D imaging system |
| CN107049242B (en) * | 2017-01-24 | 2023-08-29 | 青岛大学 | Scanning type human body microvascular ultrastructural three-dimensional imaging system |
| CN107883895A (en) * | 2017-11-21 | 2018-04-06 | 苏州睿牛机器人技术有限公司 | Multiple light courcess laser beam detecting sensor and its detection method |
| CN107883895B (en) * | 2017-11-21 | 2024-02-13 | 苏州睿牛机器人技术有限公司 | Multi-light source laser detection sensor and detection method thereof |
| CN115791756A (en) * | 2022-11-24 | 2023-03-14 | 北京杏林睿光科技有限公司 | Laser-induced breakdown spectroscopy device for measuring full spectrum at one time |
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