CN203083967U - Capillary chip electrophoresis structure for realizing multiple feeding - Google Patents
Capillary chip electrophoresis structure for realizing multiple feeding Download PDFInfo
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- CN203083967U CN203083967U CN 201220691492 CN201220691492U CN203083967U CN 203083967 U CN203083967 U CN 203083967U CN 201220691492 CN201220691492 CN 201220691492 CN 201220691492 U CN201220691492 U CN 201220691492U CN 203083967 U CN203083967 U CN 203083967U
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Abstract
The utility model relates to a capillary chip electrophoresis structure for realizing multiple feeding. The capillary chip electrophoresis structure comprises a quartz capillary tube, an anode liquid tank, a sample tank and a cathode liquid tank, wherein two ends of the quartz capillary tube are respectively coated with metal to form an anode and a cathode, the anode and the cathode are respectively connected with a high-voltage power supply through a conducting wire, the anode liquid tank is in sealing connection with an anode end of the quartz capillary tube, the positions of the anode liquid tank, the high-pressure power supply and the quartz capillary tube are not fixed; and the sample tank and the cathode liquid tank are fixed on a one-dimensional moving device, and the lower end of each of the sample tank and the cathode liquid tank is respectively provided with a notch for the quartz capillary tube to rapidly sweep away. An electrode is integrated with an electrophoresis passage, the quartz capillary tube is used as an electrophoresis passage, and two ends of the quartz capillary tube are respectively coated with a metal layer which is used as the electrode. The liquid storage tank is separated from the electrophoresis passage, so that the electrophoresis passage is released and can move freely, and the problem that a plurality of samples cannot be rapidly fed by adopting a single electrophoresis passage can be solved.
Description
Technical field
The utility model relates to a kind of capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction, belongs to electrophoresis and microflow control technique field.
Background technology
Capillary Electrophoresis (CE) claim high performance capillary electrophoresis (HPCE again, High Performance Capillary Electrophoresic) or kapillary electricity partition method (CESM), be that ion or charged particle are driving force with the high-voltage dc, in kapillary by its mobility or/and the partition factor difference is carried out efficiently, a kind of new technology of separating fast.Compartment analysis researchs such as fractionation, protein, polypeptide analysis, the separation detection of neurotransmitter class material, the separation of oligonucleotides, the DNA order-checking that high performance capillary electrophoresis has been used for separation detection, the amino acid enantiomer of saccharide compound separates with the DNA restriction fragment.
Jorgenson and Lukacs in the fused quartz kapillary of use 75 μ m internal diameters in 1981, adopt electromigration injection and sensitive fluoroscopic examination, produce 4 * 10 under 30KV voltage
5The theoretical cam curve of/m, high like this post is imitated becomes the epoch-making milestone of high performance capillary electrophoresis.At present, international HPCE research lays particular emphasis on application, and the research of new clastotype and coupling technique also obtains many breakthroughs.
The HPCE structure, in widely used capillary zone electrophoresis, be filled with the background electrolyte (buffer solution) of same composition and concentration in kapillary and the slot electrode, sample imports from an end capillaceous (being called the sample introduction end), after the kapillary two ends added certain voltage, charged solute just moved towards the electrode direction with its opposite polarity.Since the mobility difference between the sample different component, the migration rate difference, thereby through behind the certain hour, each component will arrive detecting device and be detected successively by its speed (mobility) size order, obtains the electrophoresis spectrogram that distributes by the time.This spectrogram is similar to the elution curve of chromatogram, and a component is represented at each peak, and transit time is similar to the retention time of chromatogram, can do qualitative analysis, and spectrum peak heights or area can be done quantitative test.But the shortcoming of this traditional fused quartz Capillary Electrophoresis has: 1) quartz capillary is very long, electrophoresis chronic; 2) two end electrodes groove capillaceous length very big and capillaceous has caused the electrophoresis apparatus volume very big; 3) because length capillaceous causes the voltage of energising very high; 4) sample size is difficult to control.
Because above shortcoming, developed the chip capillary cataphoresis technology afterwards, so-called chip capillary cataphoresis technology is exactly that sample preparation, sample introduction, separation, detection all are integrated in a microscale experiment chamber technology on more than one square centimeters the chip, claim integrated capillary electrophoretic chip (Integrated Capillary Electrophoresis Chips, ICE chip) again.This technology is expected to develop into the mainstream technology of micro-total analysis system and chip lab.Research and widespread use will make many fields such as DNA and protein detection, environmental monitoring, new drug research exploitation, food safety detection produce revolutionary variation.
1) material of capillary electrophoresis chip and structure
The matrix material of capillary electrophoresis chip has quartz, glass, silicon chip, superpolymer, pottery and silicon rubber etc. several, glass is to use maximum chip materials at present, main and its good optical character, thermal diffusivity and insulativity that is had of successful Application and studied thorough surface nature, the micro-processing method of 40 years these materials has obtained ripe development at microelectronic in the past.The ICE chip technology is to grow up on semi-conductive micro-fabrication technology basis.With glass is the capillary electrophoresis chip manufacture process of substrate, generally passes through deposition, photoetching, etching and bonding four step process.Though the employing dry etching can be at the microchannel of acquisition high-aspect-ratio on glass, the surface ratio of pipeline is more coarse, thereby has reduced the separation efficiency of electrophoresis.So the method that generally adopts wet etching is in the smooth canalicular channel of making on glass.Do the matrix material low price with plastic material, good insulation performance is arranged, can apply high electric field and realize separating fast, be shaped easily, it is low to produce cost in batches, easily obtains the microstructure of high-aspect-ratio, and the p H of electroosmotic flow and solution is irrelevant substantially, has broad application prospects.The most frequently used in the silicon rubber is dimethyl silicone polymer (PDMS), and its advantage has: low price, can be mass-produced, prepare weak point easy, consuming time, encapsulation easily.The more poor than signal to noise ratio (S/N ratio) with glassy phase of high polymer material is the current problem that faces.
The channel depth of capillary electrophoresis chip is generally 10～100 μ m, and width is 30～200 μ m.The structure that electrophoresis chip separates the microchannel mainly contains linear pattern, spirality, polygon, crooked shape etc.Wherein rectilinear structure is simple, separation efficiency is high, but is only applicable to short split tunnel, and spirality has bigger radius-of-curvature, and separation efficiency is higher, is suitable for long split tunnel.The polygonized structure compactness, but have the dead volume district at the flex point place, separation efficiency is reduced.Crooked shape has the characteristics of compact conformation, but because " the runway effect " in knee will make the broadening of sample area band.Along with going deep into of people's research, number of channels also is being on the increase, from initial single channel structure, and 8 passages finally, 12 passages, 16 passages, 48 passages, 96 passages develop into 384 present passages.
2) sample introduction of chip capillary cataphoresis
In chip capillary cataphoresis, sample size is generally the pL level, requirement to sample introduction is relatively stricter, minimum microscopic capillary channel dimensions has more increased the sample introduction difficulty, therefore for reaching efficient separation detection purpose fast, what adopt mostly now is two-dimentional cruciform shape injection port structure under the electromigration injection pattern, and the advantage of this structure is to realize online sample introduction by voltage transitions, get rid of external interference, be easy to carry out quantitative test.In chip capillary cataphoresis, annotate the sample zone because sample intake passage and split tunnel have intersected to form one, so its input mode and traditional Capillary Electrophoresis there is certain difference.
The chip capillary cataphoresis input mode mainly is to adopt electromigration injection, electromigration injection is divided into " suspension sample introduction " " extraining sampling " and " gate-type sample introduction " again, when being sample introduction, the suspension sample introduction adds certain voltage at sample cell, sample enters split tunnel under the function of current, after treating into intact sample, high pressure is turned to Buffer Pool, and sample begins to separate.The sample size of suspension sample introduction is relevant with added voltage, and the material sample introduction that mobility is big is just fast, exists electromigration injection to discriminate against.When then being sample introduction, extraining sampling is applied with certain voltage respectively at sample cell, buffer pool and each two ends, pond of separating waste liquid pool, with sample waste liquid pool ground connection, suitable electric field intensity can reduce the electromigration injection discrimination when controlling sample introduction by adding different voltage, improved the accuracy of sample introduction, extraining sampling can prevent effectively that sample is to the leakage of split tunnel in the diffusion of sample band and the specimen access.And the gate-type sample introduction is all to be added with voltage on each pond, and separation voltage is added in a side of split tunnel front end and Buffer Pool, and when starting high pressure, the part high-pressure spray is to sample cell, and this moment, sample waste liquid pool and detection cell must ground connection.This input mode can carry out continuous sample introduction, but also exists bigger sample to discriminate against.
Though, increased operation complexity because chip capillary cataphoresis has solved the big sample size of the volume problem of control easily.Electrode is increased to four, liquid storage tank by two and also is increased to four, brings very big difficulty for like this cleaning of chip.
No matter be traditional Capillary Electrophoresis or the capillary electrophoresis chip that developed afterwards, it is exactly all to need electrode that a common problem is all arranged, and all electrode will be inserted in the electrode solution or slot electrode at passage two ends, separates with electrophoresis path.
The utility model content
The purpose of this utility model is to overcome the deficiency that prior art exists, and a kind of repeatedly sample introduction capillary core chip electrophoresis structure that is used to realize is provided.
The purpose of this utility model is achieved through the following technical solutions:
Be used to realize repeatedly the capillary core chip electrophoresis structure of sample introduction, characteristics are: comprise quartz capillary, anode liquid pool, sample cell and negative electrode liquid pool, the two ends of described quartz capillary are coated with metal level respectively, form anode and negative electrode, anode links to each other with high-voltage power supply by lead respectively with negative electrode, the anode tap of described anode liquid pool and quartz capillary is tightly connected, and the position of described anode liquid pool, high-voltage power supply and quartz capillary is all fixing; Described sample cell and negative electrode liquid pool are fixed on the one dimension mobile device, and the lower end of sample cell and negative electrode liquid pool is equipped with for the quick inswept breach of quartz capillary.
Further, the above-mentioned capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction, wherein, described one dimension mobile device is provided with many group sample cells and anode liquid pool, and the lower end of each sample cell and negative electrode liquid pool is equipped with for the quick inswept breach of quartz capillary.
Further, above-mentioned being used to realizes repeatedly the capillary core chip electrophoresis structure of sample introduction, and wherein, the length of described quartz capillary is 5cm～20cm; The internal diameter of described quartz capillary is 25～150 microns, and external diameter is 100～500 microns.
Further, above-mentioned being used to realizes repeatedly the capillary core chip electrophoresis structure of sample introduction, and wherein, the width of described breach is 0.5～2mm.
Again further, above-mentioned being used to realizes repeatedly the capillary core chip electrophoresis structure of sample introduction, and wherein, described metal level is gold layer, silver layer, copper layer, aluminium lamination, nickel dam or platinum layer; Described metal layer thickness is 10nm～20 μ m.
The substantive distinguishing features of technical solutions of the utility model and progressive being mainly reflected in:
Electrode and electrophoresis path are integrated, and are electrophoresis path with the fused quartz kapillary of 10cm, plate metal level at the two ends of quartz capillary, as electrode.Because thereby liquid storage tank separates with electrophoresis path and has liberated electrophoresis path, it can be moved freely, not only solve the problem that single electrophoresis path can not carry out various product sample introduction fast, problems such as the electrode that also solves chip capillary cataphoresis is many, liquid storage tank many, cleaning difficulty.The utility model detects flux and greatly improves, and can per hour detect tens, hundreds of even thousands of samples.Need sample size few, the sample consumption is 0.3nl only.Sample cell and catholyte cell compartment are arranged, and effectively avoid polluting.
Description of drawings
Below in conjunction with accompanying drawing technical solutions of the utility model are described further:
Fig. 1: structural representation of the present utility model.
Embodiment
As shown in Figure 1, be used to realize repeatedly the capillary core chip electrophoresis structure of sample introduction, comprise quartz capillary 3, anode liquid pool 6, sample cell 4 and negative electrode liquid pool 5, the length of quartz capillary 3 is 5cm～20cm; The internal diameter of described quartz capillary is 25～150 microns, external diameter is 100～500 microns, the polyimide external coating at quartz capillary two ends removes, remove length range 1mm～20mm, the two ends of quartz capillary 3 are coated with metal level respectively, form anode 1 and negative electrode 2, metal level is the gold layer, silver layer, the copper layer, aluminium lamination, nickel dam or platinum layer, metal layer thickness is 10nm～20 μ m, anode 1 links to each other with high-voltage power supply by lead respectively with negative electrode 2, anode liquid pool 6 is tightly connected anode liquid pool 6 with the anode tap of quartz capillary 3, the position of high-voltage power supply and quartz capillary 3 is all fixing; Sample cell 4 and negative electrode liquid pool 5 are fixed on the one dimension mobile device, and the lower end of sample cell 4 and negative electrode liquid pool 5 is equipped with for the quick inswept breach of quartz capillary, and the width of breach is 0.5～2mm.
More preferably, the one dimension mobile device is provided with many group sample cells and anode liquid pool, and the lower end of each sample cell and negative electrode liquid pool is equipped with for the quick inswept breach of quartz capillary.Can make the inswept fast a plurality of samples of quartz capillary, realize the various product of fast detecting.
When said apparatus is used to detect, at first, in quartz capillary 3, add screening glue at anode liquid pool 6 places with syringe, anode 1 is connected with high-voltage power supply respectively with negative electrode 2, after mobile one dimension mobile device makes quartz capillary 3 aim at negative electrode liquid pool 5, open high-voltage power supply, the voltage of high-voltage power supply is 1000～10000V, then, the one dimension mobile device makes the indentation, there of quartz capillary 3 quick inswept sample cells 4 with the speed fast moving of 1mm/s～10m/s, enter next negative electrode liquid pool (another group cathode pool and sample cell, in theory can infinite expanding) and stopped 1～10 minute, sample electrophoresis in the negative electrode liquid pool is finished.
The utility model is creationary to integrate electrode and electrophoresis path, is electrophoresis path with the fused quartz kapillary of 10cm, plates metal level at the two ends of quartz capillary, as electrode.Because thereby liquid storage tank separates with electrophoresis path and has liberated electrophoresis path, it can be moved freely, not only solve the problem that single electrophoresis path can not carry out various product sample introduction fast, problems such as the electrode that also solves chip capillary cataphoresis is many, liquid storage tank many, cleaning difficulty.
The utility model detects flux and greatly improves, and can per hour detect tens, hundreds of even thousands of samples.Need sample size few, the sample consumption can reach 0.3nl.Sample cell and catholyte cell compartment are arranged, and can not cause pollution.
What need understand is: the above only is a preferred implementation of the present utility model; for those skilled in the art; under the prerequisite that does not break away from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (7)
1. be used to realize repeatedly the capillary core chip electrophoresis structure of sample introduction, it is characterized in that: comprise quartz capillary, anode liquid pool, sample cell and negative electrode liquid pool, the two ends of described quartz capillary are coated with metal level respectively, form anode and negative electrode, anode links to each other with high-voltage power supply by lead respectively with negative electrode, the anode tap of described anode liquid pool and quartz capillary is tightly connected, and the position of described anode liquid pool, high-voltage power supply and quartz capillary is all fixing; Described sample cell and negative electrode liquid pool are fixed on the one dimension mobile device, and the lower end of sample cell and negative electrode liquid pool is equipped with for the quick inswept breach of quartz capillary.
2. the capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction according to claim 1, it is characterized in that: described one dimension mobile device is provided with many group sample cells and anode liquid pool, and the lower end of each sample cell and negative electrode liquid pool is equipped with for the quick inswept breach of quartz capillary.
3. the capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction according to claim 1, it is characterized in that: the length of described quartz capillary is 5cm～20cm.
4. the capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction according to claim 1, it is characterized in that: the internal diameter of described quartz capillary is 25～150 microns, external diameter is 100～500 microns.
5. the capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction according to claim 1 and 2, it is characterized in that: the width of described breach is 0.5～2mm.
6. the capillary core chip electrophoresis structure that is used to realize repeatedly sample introduction according to claim 1 is characterized in that: described metal level is gold layer, silver layer, copper layer, aluminium lamination, nickel dam or platinum layer.
7. according to claim 1 or the 6 described capillary core chip electrophoresis structures that are used to realize repeatedly sample introduction, it is characterized in that: described metal layer thickness is 10nm～20 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220691492 CN203083967U (en) | 2012-12-14 | 2012-12-14 | Capillary chip electrophoresis structure for realizing multiple feeding |
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| CN 201220691492 CN203083967U (en) | 2012-12-14 | 2012-12-14 | Capillary chip electrophoresis structure for realizing multiple feeding |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018310A (en) * | 2012-12-14 | 2013-04-03 | 凯晶生物科技(苏州)有限公司 | Capillary chip electrophoresis structure for implementing multiple sample injection and implementation method thereof |
| CN108593748A (en) * | 2018-01-26 | 2018-09-28 | 南京溯远基因科技有限公司 | capillary and DNA sequencer |
-
2012
- 2012-12-14 CN CN 201220691492 patent/CN203083967U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018310A (en) * | 2012-12-14 | 2013-04-03 | 凯晶生物科技(苏州)有限公司 | Capillary chip electrophoresis structure for implementing multiple sample injection and implementation method thereof |
| CN108593748A (en) * | 2018-01-26 | 2018-09-28 | 南京溯远基因科技有限公司 | capillary and DNA sequencer |
| CN108593748B (en) * | 2018-01-26 | 2024-04-30 | 南京溯远基因科技有限公司 | Capillary and DNA sequencer |
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| C14 | Grant of patent or utility model | ||
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Addressee: Kaijing Biological Technology (Suzhou) Co., Ltd. Document name: Notification that Application Deemed not to be Proposed |
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| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Kaijing Bio-Technology (Suzhou) Co.,Ltd. Document name: Deemed not to advise |
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| ASS | Succession or assignment of patent right |
Owner name: ZHANG YINGPIN Free format text: FORMER OWNER: KAIJING BIOLOGICAL TECHNOLOGY (SUZHOU) CO., LTD. Effective date: 20140113 |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Free format text: CORRECT: ADDRESS; FROM: 215123 SUZHOU, JIANGSU PROVINCE TO: 650200 KUNMING, YUNNAN PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20140113 Address after: 650200, No. 2, unit 5, 31 Tung Wan, Guandu District, Yunnan, Kunming Patentee after: Zhang Yingpin Address before: Suzhou City, Jiangsu province 215123 Park Linquan Street No. 399 Patentee before: Kaijing Biological Technology (Suzhou) Co., Ltd. |
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| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130724 Termination date: 20131214 |