CN202916285U - Whole blood immunochromatography device - Google Patents
Whole blood immunochromatography device Download PDFInfo
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- CN202916285U CN202916285U CN 201220594022 CN201220594022U CN202916285U CN 202916285 U CN202916285 U CN 202916285U CN 201220594022 CN201220594022 CN 201220594022 CN 201220594022 U CN201220594022 U CN 201220594022U CN 202916285 U CN202916285 U CN 202916285U
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- whole blood
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Abstract
The utility model relates to a whole blood immunochromatography device which comprises an upper housing, a lower housing and a test strip, wherein the housings are arranged up and down in a fastening manner; the test strip is arranged between the housings; a sample feeding hole and an observation window are arranged on the upper housing; a clamping groove for fixing the test strip is formed in the lower housing; the test strip comprises a sample feed area, a hemoglobin adsorption area, a binding area, a detection area and a water absorption area which are overlapped on a support; the hemoglobin adsorption area is covered by the sample feed area completely, and comprises a hemoglobin adsorption pad and microspheres; and the microspheres are filled in the hemoglobin adsorption pad and coated by hemoglobin antibodies. The whole blood immunochromatography device has the advantages that disturbance of hemolysis to a whole blood detection result can be eliminated; the separation effect is stable; the operation is simple; the sample amount is low; and the detection speed is high.
Description
Technical field
The utility model relates to technical field of immunoassay, is specifically related to a kind of whole blood immunity chromatographic apparatus.
Background technology
Immunochromatography is a kind of express-analysis technology that grows up early 1980s, it is often take the fibre strip chromatographic material as solid phase, by capillary action sample solution is flowed at chromatography strip, and make simultaneously determinand in the sample and the antigen on the chromatographic material or antibody that high special immune response occurs to form immune complex, immune complex is by enrichment or be trapped in and contain the detection region that specificity is caught composition in the chromatography process, the label (such as collaurum) that can estimate by enzyme reaction or direct utilization obtains intuitively experimental phenomena, such as colour developing.Free label is then crossed and is detected band, reaches the purpose of automatically separating with binding label.Perhaps, the determinand in the sample also can be combined with the mark tracer first, passes through chromatography process again, arrives to detect to be with the position to obtain testing result with catching composition generation specific binding.This analytical technology is simple to operate fast, and analysis result is clear, be easy to judge, and need not instrument (or only needing simple instrument), fast detecting is carried out at the scene of being adapted at, thereby is widely used in quick clinical diagnosis field.
Clinical diagnosis detects and usually adopts whole blood as detecting sample fast, and serum or plasma sample need to leave standstill a period of time because of preparation or need the centrifugal time lengthening that obtains testing result that makes, therefore often be not suitable as the sample of fast detecting.Yet, because being used for easily occurring when immunochromatography detects red blood cell, filters not exclusively or haemolysis whole blood sample, cause haemoglobin that red blood cell and red blood cell discharge with the analyte chromatography to detection zone, the background color of detection window is increased, disturb so can reach qualitative, the sxemiquantitative of detection line quantitatively to detect to produce.Based on above problem, the present immune chromatography reagent kit polyester material that adheres to certain pore size on the sample pad of being everlasting filters haemocyte, such as patent CN201935921U and CN2629040Y, this method needs relatively large blood when detecting, and velocity of separation is slow, and this method can not be eliminated the impact that haemolysis produces; Another kind method is to drip in advance on the application of sample pad or the absorption blood coagulation accelerator, make red cell agglutination agglomerating, can not be by filtering material and small-bore film, such as patent CN1243251A and CN101614736A, but this kind method is because of the set accelerator One's name is legion, it is also different that different types of abiotic active set accelerator and biologically active set accelerator are tired, and it is dilution determines comparatively trouble, and this method is easy to occur haemolysis when stoping haemocyte by filtering material.
Summary of the invention
The characteristics such as the utility model provides a kind of whole blood immunity chromatographic apparatus that can fully filter red blood cell and solve the haemolysis interference problem for the deficiencies in the prior art, and it has accurately, antijamming capability is strong, easy and quick.
The utility model can be achieved through the following technical solutions:
A kind of whole blood immunity chromatographic apparatus, by the housing that fastens up and down with place test strips therebetween to form, described upper shell is provided with well and view window; Described lower house is provided with the fixedly draw-in groove of test strips; Described test strips is included in sample introduction district, haemoglobin adsorption zone, land, detection zone and the suction zones that mutually overlaps on the stilt, described haemoglobin adsorption zone is covered fully by the sample introduction district, and described haemoglobin adsorption zone comprises haemoglobin absorption layer and the coated microballoon of hemoglobin antibodies.
Described sample introduction district's right-hand member and haemoglobin adsorption zone right-hand member consistency from top to bottom guarantee that sample is vertically smoothly fast by the haemoglobin adsorption zone.Described sample introduction district is made by one or more materials that are selected from polyester film, glass fibre element film, cellulose filter paper and the nonwoven fabrics.In certain embodiments, described sample introduction district is also adsorbable a blood anticoagulant.
The coated microballoon of described hemoglobin antibodies is filled in the haemoglobin absorption layer, and the coated microballoon of described hemoglobin antibodies is selected from a kind of in collaurum, colloidal-carbon, electroselenium, magnetic particle and the polystyrene microsphere, preferred polystyrene microsphere.Being connected and can being undertaken by physisorption or chemical coupling mode between described hemoglobin antibodies and the microballoon, preferred chemical coupling mode.
Described haemoglobin adsorption zone also comprises 1～3 layer of diaphragm that is positioned at haemoglobin absorption layer bottom; the aperture of described diaphragm is less than the diameter of described microballoon; the effect of diaphragm mainly is fixing microballoon, prevents that microballoon from entering the zone beyond the haemoglobin adsorption zone.Described diaphragm is made by one or more materials that are selected from polyester film, glass fibre element film, cellulose filter paper and the nonwoven fabrics.
Described land can be comprised of glass fibre element film or polyester film, is coated with analyte antibody or the antigen of label mark on it, and described label is metal colloid particles, fluorescent grain, magnetic-particle or coloured latex particle.Described detection zone can be made of nitrocellulose filter, polyvinylidene fluoride film or cellulose acetate membrane, its nearly land one side and nearly suction zones one side are respectively arranged with detection line and nature controlling line, detection line and nature controlling line are spaced from each other a distance, described detection line be coated with can with sample in antibody or the antigen of analyte response.Described suction zones can be made with materials such as cellulose, glass fibre, porous polyethylene or porous polypropylenes.
Also can comprise an information track on the described upper shell, described information track can be used for depositing release, can put down in writing the information such as patient's numbering, testing result and date on the release, it can be taken out separately after the detection and preserve as patient's data.
Described upper-lower casing material is made by the plastics that do not absorb water, comprise artificial synthetic or nature organic material, such as polystyrene, tygon, Polyvinylchloride, polyester or cellulose and derivant thereof etc., or use a kind of plastic material or use several plastic materials physically stack or addition after compound.
Except immune double antibodies sandwich method, described whole blood immunity chromatographic apparatus also is applicable to Immune competition and suppresses method to the detection of sample.
Detection principle of the present utility model is specially: whole blood sample is dripped in well, erythrocytic effect in the filtering blood can play in the sample introduction district, blood after the filtration flows into the haemoglobin adsorption zone, the haemoglobin adsorption zone is filtering blood and adsorb in the blood haemoglobin that may produce because of erythrocyte fragmentation further, blood continues chromatography to land is combined with the antibody that is coated with label or antigentic specificity, arrives at last detection zone and is combined with corresponding capture antibody or antigen.Analyte antigen or antibody concentration are directly proportional and realize qualitative, sxemiquantitative or quantitatively detection to target analytes in the label-antibody that forms by the detection zone place-Ag-Ab or label-Ag-Ab-antigenic compound and the sample.
The beneficial effects of the utility model are mainly reflected in:
1, the impact that in the time of can eliminating whole blood test generation haemolysis the result is caused, the adsorbable haemoglobin because of the erythrocyte fragmentation generation of haemoglobin adsorption zone, avoid its exhibition layer to detection zone, increase background color, affect the reviewer to the color judgement of test strip and the instrument signal scanning to test strip.
2, have a separating effect stable, simple to operate, the sample consumption is few and detect the advantages such as rapid.
Description of drawings
Fig. 1 is upper shell structural representation of the present utility model;
Fig. 2 is lower house structural representation of the present utility model;
Fig. 3 is test strips structural representation of the present utility model.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail:
1 one kinds of collaurum whole blood immunities of embodiment chromatographic apparatus
A kind of collaurum whole blood immunity chromatographic apparatus, formed by upper shell (1), lower house (2) and test strips (3), upper shell (1) and lower house (2) can closely fasten, wherein upper shell (1) is provided with well (4), view window (5) and information track (6), well (4) is oval funnel-form, there is downward-sloping inclined-plane in sample introduction district (7) corresponding to test strips (3), can prevent overflowing of Hemostatic Oral Liquid; View window (5) is corresponding to the detection zone (8) of test strips (3); Information track (6) is inlaid with the patient information card, and the above has the information such as patient's numbering, result and date, and the patient information card can take out separately as patient's data and preserve.Be provided with the draw-in groove (9) that mates with test strips (3) shape size in the lower house (2), be convenient to fixedly test strips (3).Above-mentioned upper shell (1) and lower house (2) are made by waterproof PVC material.
Test strips (3) comprises that from left to right overlap joint successively sticks on sample introduction district (7), haemoglobin adsorption zone (11), land (12), detection zone (8) and the suction zones (13) on the stilt (10).Wherein, stilt (10) is made of the PVC plate; Sample introduction district (7) is comprised of glass fibre membrane, and absorption has blood anticoagulant on the glass fibre membrane; Haemoglobin adsorption zone (11) is positioned at below the sample introduction district (7), covered fully by sample introduction district (7), and coated microballoon and one deck of hemoglobin antibodies that its right-hand member and sample introduction district (7) right-hand justified, described haemoglobin adsorption zone (11) comprise the haemoglobin absorption layer, be filled in the absorption layer is positioned at the undermost diaphragm of adsorption zone; Land (12) is comprised of polyester film, and the above is coated with the target antigen monoclonal antibody of colloid gold label, and the right side overlap joint of the left side of described land (12) and haemoglobin adsorption zone (11) is pasted, and two region overlapping length are 1mm; Detection zone (8) is made of nitrocellulose membrane, the nearly land of detection zone (12) one sides and nearly suction zones (13) one sides are respectively equipped with a detection line and a nature controlling line, described detection line is coated with the target antigen polyclonal antibody, and described nature controlling line is coated with the sheep anti-mouse igg polyclonal antibody; Suction zones (13) is comprised of cellulose filter paper, and its left side overlaps bonding mutually with the right side of detection zone (8).
Whole blood sample 120 μ l to be checked are directly added the well place, blood through syphonic effect along the sample introduction district, the haemoglobin adsorption zone, land, detection zone move to the suction zones direction, read testing result in 15 minutes.
Qualitative detection:
Positive: an aubergine band respectively appears in T line and C line place;
Negative: an aubergine band only occurs at C line place;
Invalid: as no matter to contain or do not contain target antigen in the sample, the monoclonal antibody of the colloid gold label at collaurum pad place all can go upward to detection zone and be combined with the sheep anti-mouse igg polyclonal antibody at C line place, an aubergine band occurs at C line place, as not occurring, then the result is invalid, needs again to detect.
Quantitatively detect:
Device for immunochromatography is placed the collaurum instrument, and instrument is collected T line, C line signal intensity by detection zone is scanned, and finally changes, calculates the concentration of target antigen in the sample, realizes quantitatively detecting.
2 one kinds of fluorescence whole blood immunities of embodiment chromatographic apparatus
But the collaurum whole blood immunity chromatographic apparatus in the structure reference example 1 of fluorescence whole blood immunity chromatographic apparatus, the main difference part of two kinds of whole blood devices is that the land of collaurum whole blood device is coated with the monoclonal antibody of colloid gold label, and the label that adopt the land of fluorescence whole blood device is fluorescent grain.
Whole blood sample 120 μ l to be checked are directly added the well place, blood through syphonic effect along the sample introduction district, the haemoglobin adsorption zone, land, detection zone move to the suction zones direction, test card is placed fluorescence analyser, instrument is by scanning detection zone, collect T line, C line fluorescence signal intensity, finally change, calculate the concentration of target antigen in the sample, realize quantitatively detecting.
3 one kinds of magnetic bead whole blood immunities of embodiment chromatographic apparatus
But the collaurum whole blood immunity chromatographic apparatus in the structure reference example 1 of magnetic bead whole blood immunity chromatographic apparatus, the main difference part of two kinds of whole blood devices is that the land of collaurum whole blood device is coated with the monoclonal antibody of colloid gold label, and the label that adopt the land of magnetic bead whole blood device is magnetic nanoparticle.
Whole blood sample 120 μ l to be checked are directly added the well place, blood through syphonic effect along the sample introduction district, the haemoglobin adsorption zone, land, detection zone move to the suction zones direction, read testing result in 15 minutes.
Qualitative detection:
Positive: a colour developing band respectively appears in T line and C line place;
Negative: a colour developing band only occurs at C line place;
Invalid: as no matter to contain or do not contain target antigen in the sample, the monoclonal antibody of the marked by magnetic bead at place, land all can go upward to detection zone and be combined with the sheep anti-mouse igg polyclonal antibody at C line place, a colour developing band occurs at C line place, as not occurring, then the result is invalid, needs again to detect.
Quantitatively detect:
Test card is placed the magnetometric analysis instrument, and instrument is collected T line, C line magnetic signal intensity by detection zone is scanned, and finally changes, calculates the concentration of target antigen in the sample, realizes quantitatively detecting.
4 one kinds of latex whole blood immunities of embodiment chromatographic apparatus
But the collaurum whole blood immunity chromatographic apparatus in the structure reference example 1 of latex whole blood immunity chromatographic apparatus, the main difference part of two kinds of whole blood devices is that the land of collaurum whole blood device is coated with the monoclonal antibody of colloid gold label, and the label that adopt the land of magnetic bead whole blood device is coloured latex particle.
Whole blood sample 120 μ l to be checked are directly added the well place, blood through syphonic effect along the sample introduction district, the haemoglobin adsorption zone, land, detection zone move to the suction zones direction, read testing result in 15 minutes.
Qualitative detection:
Positive: a colour developing band respectively appears in T line and C line place;
Negative: a colour developing band only occurs at C line place;
Invalid: as no matter to contain or do not contain target antigen in the sample, the monoclonal antibody of the latex mark at place, land all can go upward to detection zone and be combined with the sheep anti-mouse igg polyclonal antibody at C line place, a colour developing band occurs at C line place, as not occurring, then the result is invalid, needs again to detect.
Quantitatively detect:
Test card is placed instrument, and instrument is collected T line, C line signal intensity by detection zone is scanned, and finally changes, calculates the concentration of target antigen in the sample, realizes quantitatively detecting.
It should be noted that at last: the above only is preferred embodiment of the present utility model, be not limited to the utility model, although with reference to previous embodiment the utility model is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (8)
1. whole blood immunity chromatographic apparatus, by the housing that fastens up and down with place test strips therebetween to form, it is characterized in that: described upper shell is provided with well and view window; Described lower house is provided with the fixedly draw-in groove of test strips; Described test strips is included in sample introduction district, haemoglobin adsorption zone, land, detection zone and the suction zones that mutually overlaps on the stilt, described haemoglobin adsorption zone is covered fully by the sample introduction district, and described haemoglobin adsorption zone comprises haemoglobin absorption layer and the coated microballoon of hemoglobin antibodies.
2. whole blood immunity chromatographic apparatus according to claim 1 is characterized in that, described sample introduction district's right-hand member and haemoglobin adsorption zone right-hand member consistency from top to bottom.
3. whole blood immunity chromatographic apparatus according to claim 1 is characterized in that, the coated microballoon of described hemoglobin antibodies is filled in the haemoglobin absorption layer.
4. according to claim 1 or 3 described whole blood immunity chromatographic apparatus, it is characterized in that, the coated microballoon of described hemoglobin antibodies is selected from a kind of in collaurum, colloidal-carbon, electroselenium, magnetic particle and the polystyrene microsphere.
5. whole blood immunity chromatographic apparatus according to claim 1 is characterized in that, described haemoglobin adsorption zone also comprises 1～3 layer of diaphragm that is positioned at haemoglobin absorption layer bottom.
6. whole blood immunity chromatographic apparatus according to claim 5 is characterized in that, the aperture of described diaphragm is less than the diameter of described microballoon.
7. whole blood immunity chromatographic apparatus according to claim 1, it is characterized in that, described land is coated with analyte antibody or the antigen of label mark, nearly land one side of described detection zone and nearly suction zones one side are respectively arranged with detection line and nature controlling line, described detection line be coated with can with sample in antibody or the antigen of analyte response.
8. whole blood immunity chromatographic apparatus according to claim 7 is characterized in that, described label is metal colloid particles, fluorescent grain, magnetic-particle or coloured latex particle.
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| CN 201220594022 CN202916285U (en) | 2012-11-12 | 2012-11-12 | Whole blood immunochromatography device |
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| CN 201220594022 CN202916285U (en) | 2012-11-12 | 2012-11-12 | Whole blood immunochromatography device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106526176A (en) * | 2016-12-05 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Multifunctional fluorescence-immunochromatography rapid quantitative detection strip |
| CN106546729A (en) * | 2016-10-18 | 2017-03-29 | 上海凯璟生物科技有限公司 | A kind of new process for removing serum matrix effect in the detection of dry type immunofluorescence sizing technique |
| CN109187943A (en) * | 2018-08-24 | 2019-01-11 | 四川新健康成生物股份有限公司 | The preparation method of anti-interference coating in a kind of anti-interference reagent cup and reagent cup |
| CN110736830A (en) * | 2019-11-18 | 2020-01-31 | 威尚生物技术（合肥）有限公司 | detection cards for autoimmune colloidal gold |
| CN113607944A (en) * | 2021-08-26 | 2021-11-05 | 深圳市亚辉龙生物科技股份有限公司 | Colloidal gold chromatography reagent strip, preparation method and neocorona antigen detection kit |
| CN113834944A (en) * | 2021-11-25 | 2021-12-24 | 山东子峰生物技术有限公司 | Quantum dot fluorescence detection method for folic acid in red blood cells |
-
2012
- 2012-11-12 CN CN 201220594022 patent/CN202916285U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106546729A (en) * | 2016-10-18 | 2017-03-29 | 上海凯璟生物科技有限公司 | A kind of new process for removing serum matrix effect in the detection of dry type immunofluorescence sizing technique |
| CN106526176A (en) * | 2016-12-05 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Multifunctional fluorescence-immunochromatography rapid quantitative detection strip |
| CN109187943A (en) * | 2018-08-24 | 2019-01-11 | 四川新健康成生物股份有限公司 | The preparation method of anti-interference coating in a kind of anti-interference reagent cup and reagent cup |
| CN109187943B (en) * | 2018-08-24 | 2021-07-30 | 四川新健康成生物股份有限公司 | Anti-interference reagent cup and preparation method of anti-interference coating in reagent cup |
| CN110736830A (en) * | 2019-11-18 | 2020-01-31 | 威尚生物技术（合肥）有限公司 | detection cards for autoimmune colloidal gold |
| CN113607944A (en) * | 2021-08-26 | 2021-11-05 | 深圳市亚辉龙生物科技股份有限公司 | Colloidal gold chromatography reagent strip, preparation method and neocorona antigen detection kit |
| CN113607944B (en) * | 2021-08-26 | 2022-07-08 | 深圳市亚辉龙生物科技股份有限公司 | Colloidal gold chromatography reagent strip, preparation method and neocorona antigen detection kit |
| CN113834944A (en) * | 2021-11-25 | 2021-12-24 | 山东子峰生物技术有限公司 | Quantum dot fluorescence detection method for folic acid in red blood cells |
| CN113834944B (en) * | 2021-11-25 | 2022-03-22 | 山东子峰生物技术有限公司 | Quantum dot fluorescence detection method for folic acid in red blood cells |
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