CN201589735U - On-line multi-channel immediate solid-phase extraction device - Google Patents
On-line multi-channel immediate solid-phase extraction device Download PDFInfo
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- CN201589735U CN201589735U CN2010203018860U CN201020301886U CN201589735U CN 201589735 U CN201589735 U CN 201589735U CN 2010203018860 U CN2010203018860 U CN 2010203018860U CN 201020301886 U CN201020301886 U CN 201020301886U CN 201589735 U CN201589735 U CN 201589735U
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Abstract
The utility model discloses an on-line multi-channel immediate solid-phase extraction device, comprising a vacuum pump, a vacuum pump on one side surface of the vacuum pump, a vacuum meter, a vacuum meter gauge, a vacuum adjustment valve and a vacuum pump air-exhaust nozzle and two parallel stainless steel plates on the outer surfaces at the two opposite sides perpendicular to the vacuum pump, wherein a semicircle plastic clamp is fixed on each stainless steel plate and a glass cylindrical tube is arranged between two semicircle plastic clamps at each side and a glass cylindrical tube air-exhaust nozzle is arranged on the glass cylindrical tube and a solid phase extraction channel communicated with the glass cylindrical tube is welded on each glass cylindrical tube and two ends of the solid phase extraction channel project from the glass cylindrical tube and one end of the solid phase extraction channel is provided with a solid phase extraction pillar for housing the agent and a glass plug-in unit for communicating the agent and connected with the solid phase extraction pillar is arranged in the solid phase extraction channel. The extraction device has features of simple structure, low cost, no cross pollution and high recovery.
Description
Technical field
The utility model belongs to food, medicine and environmental sample pretreatment technology field, is specifically related to the instant type solid-phase extraction device of a kind of online hyperchannel.
Background technology
Solid-Phase Extraction (Solid-Phase Extraction, be called for short SPE) be developed recently a kind of sample pretreatment technology of getting up, by liquid-solid extraction and column liquid chromatographic technology in conjunction with development, be mainly used in separation, the purifying and concentrated of sample, the recovery, the minimizing reagent use amount of comparing with traditional liquid-liquid extraction method and can improve analyte are more effectively separated analyte with interfering component, reduce the sample pretreatment process, simple to operate, save time, laborsaving.Be widely used in fields such as medicine, food, environmental protection, commodity inspection, residues of pesticides.Solid-Phase Extraction is a kind of of many uses and more and more welcome sample pre-treatments technology.
Principle: Solid-Phase Extraction is a physical chemistry extraction process that comprises liquid phase and solid phase.In the Solid-Phase Extraction process, solid phase to the absorption affinity of analyte greater than the sample mother liquor, when sample during by solid-phase extraction column, analyte is attracted to solid surface, other components are then passed through pillar with the sample mother liquor, and are last, select appropriate solvent that sample analytes is eluted again.Thereby, reach the pre-service requirement of sample separation enrichment.
Existing solid-phase extraction device is made of solid phase extraction column and servicing unit, generally is divided into device for automatically controlling and manual overvide.
Full-automatic solid-phase extraction device has automaticity height, good reproducibility, need not to keep an eye on, and mechanical arm is from advantages such as line operates.Save time, increase work efficiency.But also have the following disadvantages, one, complex structure costs an arm and a leg, and running program is complicated relatively, needs special training and certain use experience; Two, the distance between extraction sample and the extraction column is longer, causes the absorption of tube wall to analyte in the extraction sample easily, and when particularly volatile organism being analyzed, its adsorbability is more obvious, thereby, impact analysis result's accuracy.Three, sample size relatively more for a long time, and is with the obvious advantage, but works as sample number below 5, and time-consuming trouble then seems; And in a single day running program is set, in service can't the change, and operating cost is bigger.
The general configuration of existing manual solid-phase extraction equipment comprises polyethylene cover, glass cabin body, test tube rack, vacuum meter, exhaust-valve, vacuum pump, surge flask etc.Have simple in structure, advantage such as price is lower, the groundwork process is: accept test tube and test tube rack and place glass cabin body,, a plurality of solid phase extraction column joints are arranged on the polyethylene cover with the polyethylene cover sealing, but the corrosion of acid and alkali resistance, organic solvent, oxygenant etc.Bled by vacuum pump, make the body decompression of glass cabin, vacuum tightness can be monitored and regulate to pressure vacuum gauge and by-pass cock.Exist some shortcomings to be: one, all sample receiver popes all place in the same vacuum chamber body, carry out solid-phase extraction column activation, in the processes such as sample, drip washing, wash-out, to constantly vacuumize, discharge vacuum, take off or cover the tygon gland bonnet, change multistep repeating steps such as receiver pope, complex operation, especially when handling the faults such as obstruction of one of them pillar generation, the work of whole device all must be ended.Two, can only handle small volumes of sample.Three, be subjected to the restriction of receiver pope volume, the finite volume of the activation of solid phase extraction column, drip washing, wash-out agents useful for same.
Summary of the invention
The purpose of this utility model provides the instant type solid-phase extraction device of a kind of online hyperchannel, solved existing full-automatic solid-phase extraction device complex structure, cost an arm and a leg, operate complicated, the problem of the finite volume of manual solid-phase extraction equipment complex operation, agents useful for same.
The technical scheme that the utility model adopted is, the instant type solid-phase extraction device of a kind of online hyperchannel, comprise vacuum pump, one side surface of vacuum pump is provided with the vacuum-pump power supply switch, vacuum meter, vacuum breaker and the vacuum pump mouth of bleeding, relative two side external surfaces perpendicular to vacuum pump, each side external surface is provided with two parallel corrosion resistant plate supports, be fixed with semicircle plastics clip on each corrosion resistant plate support, be provided with a glass cylindrical pipe between two semicircle plastics clips of each side, the glass cylindrical pipe is provided with the glass cylindrical pipe mouth of bleeding, be welded with the Solid-Phase Extraction passage that communicates with the glass cylindrical pipe on each glass cylindrical pipe, the glass cylindrical pipe is stretched out at the two ends of Solid-Phase Extraction passage, one end of Solid-Phase Extraction passage is provided with the solid phase extraction column that is used to hold reagent, and interior being connected with solid phase extraction column of Solid-Phase Extraction passage is provided with the glass plug that is used for the reagent circulation.
Characteristics of the present utility model also are,
The outer surface of cupular part of vacuum pump wherein is provided with the vacuum pump carrying handle that is used to move.
Parallelly on each glass cylindrical pipe wherein be welded with 4-6 Solid-Phase Extraction passage.
The suitable for reading of Solid-Phase Extraction passage wherein is the frosted mouth, and end opening is the frosted plug.
Glass plug wherein is that internal diameter is the glass tube of 5mm, and the glass tube end stretches out the outer 15-20mm of Solid-Phase Extraction passage end opening.
The beneficial effects of the utility model are,
(1) simple in structure, easy operating only needs simple training.Single covering device operation can be carried out the processing of 1-12 sample simultaneously, increases work efficiency greatly.Design briefly, safety economy is installed simply, and easy to use, carry at the scene of being easy to.
(2) with low cost, device is practical, and except that vacuum pump, other assembly is a glassware, is easy to preparation and replacing; The generally accepted standard port design, minimum maintenance requirements.
(3) solid phase extraction column size and capacity are unrestricted, and reagent dosage is regulated arbitrarily, the design of control tuning knob back, and handled easily, panel is clean and tidy.
(4) the extraction passage is the manufacturing of overall height boron glass, and printing opacity is strong, visual good, neat and artistic, and analyte is not had absorption, is not subjected to the influence of solvent species.Autonomous channel extraction, no cross pollution.
(5) quantitatively purify, the distance of sample and solid-phase extraction column is short, reduces the loss of sample; Purification speed can artificially be controlled, be regulated to the concrete condition of solution, the recovery of extraction height per sample.
Description of drawings
Fig. 1 is the structural representation of the instant type solid-phase extraction device of the online hyperchannel of the utility model;
Fig. 2 is the working state schematic representation of the instant type solid-phase extraction device of the online hyperchannel of the utility model.
Among the figure, 1. vacuum meter, 2. vacuum breaker, 3. vacuum-pump power supply switch, 4. vacuum pump carrying handle, 5. glass cylindrical pipe, 6. waste collection bottle, 7. solid phase extraction column, 8. the glass cylindrical pipe mouth of bleeding, 9. sample liquid receiving flask, 10. corrosion resistant plate support, 11. semicircle plastics clips, the mouth 12. vacuum pump is bled, 13. glass plugs, 14. Solid-Phase Extraction passages, 15. vacuumize rubber tube, 16. vacuum pumps.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is elaborated.
The structure of the instant type solid-phase extraction device of the online hyperchannel of the utility model, as shown in Figure 1, the vacuum pump 16 that comprises rectangular parallelepiped, one side surface of vacuum pump 16 is provided with vacuum-pump power supply switch 3, vacuum meter 1, vacuum breaker 2 and the vacuum pump mouth 12 of bleeding, the outer surface of cupular part of vacuum pump 16 is provided with the vacuum pump carrying handle 4 that is used to move, relative two side external surfaces perpendicular to vacuum pump 16, each side external surface is provided with two parallel corrosion resistant plate supports 10, be screwed semicircle plastics clip 11 on each corrosion resistant plate support 10, be set with a glass cylindrical pipe 5 between two semicircle plastics clips 11 of each side, glass cylindrical pipe 5 is provided with the glass cylindrical pipe mouth 8 of bleeding, be welded with 4-6 the Solid-Phase Extraction passage 14 that communicates with glass cylindrical pipe 5 that be arranged in parallel on each glass cylindrical pipe 5, glass cylindrical pipe 5 is stretched out at the two ends of Solid-Phase Extraction passage 14, and the suitable for reading of Solid-Phase Extraction passage 14 is the frosted mouth; End opening is the frosted plug, can connect the heart-shaped bottle of rub oral examination tube or ground.The top of Solid-Phase Extraction passage 14 is provided with solid phase extraction column 7, be provided with glass plug 13 in the Solid-Phase Extraction passage 14, glass plug 13 is the glass tube of 5mm for holding the internal diameter of welding beyond the Great Wall in frosted, and the glass tube end stretches out Solid-Phase Extraction passage 14 end opening frosteds 15-20mm beyond the Great Wall.
The course of work of the instant type solid-phase extraction device of the online hyperchannel of the utility model, as shown in Figure 2,
(1) device is installed: vacuum pump 16 transverse horizontal are placed, the end opening of the Solid-Phase Extraction passage 14 of a waste collection bottle 6 and a side is connected, the end opening of sample liquid receiving flask 9 with the Solid-Phase Extraction passage 14 of opposite side is connected, guarantees each glass component ground contact closely, non-loosening.
(2) activation: the purpose of activation is to create an environment compatible with sample solvent and remove all impurity in the post.Usually need two kinds of solvents to finish the work, first solvent is used for purifying stationary phase, and another solvent is used to set up a suitable stationary phase environment makes sample analytes obtain suitable reservation.Each activation solvent load is about the 1-2mL/100mg stationary phase.Operation: make the glass cylindrical pipe 5 that is connected with waste collection bottle 6 in the face of the operator, can weld 4-6 Solid-Phase Extraction passage on the glass cylindrical pipe 5, can place 4-6 solid phase extraction column 7 on the passage and carry out activation processing.For example activate C18 (octadecyl silane) solid phase extraction column 7 of 3mL with 5mL methyl alcohol, close the glass piston of waste collection bottle 6 earlier, the vacuum pump of vacuum pump 16 mouth 12 and the glass cylindrical pipe of glass cylindrical pipe 5 mouth 8 usefulness of bleeding of bleeding are vacuumized 15 closely knit connections of rubber tube, in solid phase extraction column 7, add the activation solvent, open vacuum-pump power supply switch 3, control vacuum breaker 2, make the activation solvent at the uniform velocity flow through solid phase extraction column 7, in waste collection bottle 6, keep solid phase extraction column 7 stationary phase not drained by control vacuum breaker 2.
(3) go up sample: sample is joined solid-phase extraction column and forces sample solution to pass through stationary phase, analyte and liquid phase sample chaff interference are retained on the stationary phase.Operation: add sample solution in the solid phase extraction column 7 after activation, open vacuum-pump power supply switch 3, control vacuum breaker 2 makes sample solution at the uniform velocity flow through solid phase extraction column 7, in waste collection bottle 6.
(4) drip washing: after analyte obtains keeping, need the drip washing stationary phase washing unwanted sample component off usually, the eluotropic strength of drip washing solvent slightly is better than or equals the sample solvent.The drip washing solvent must be tried one's best by force, washing interfering component as much as possible off, but can not arrive by force can any one analyte of wash-out degree.Solvent volume is 0.5-0.8mL/100mg.Should not use too strong solvent during drip washing, too strong solvent can wash with keeping impurity by force.Use too Weak solvent, the drip washing solvent volume is strengthened.Can change strong, Weak solvent into uses with.Operation: add the drip washing solvent in the solid phase extraction column 7 behind last sample, open vacuum-pump power supply switch 3, control vacuum breaker 2 makes the drip washing solvent at the uniform velocity flow through solid phase extraction column 7, in waste collection bottle 6.
(5) wash-out: after the drip washing, with analyte wash-out from the stationary phase, the eluting solvent consumption is generally 0.5-0.8mL/100mg.And solvent must conscientiously be selected, and solvent is too strong, and the unnecessary component of some stronger reservations is come out; Solvent too a little less than, just need more eluent wash out analyte, the concentrated effect of solid-phase extraction column will weaken like this.Operation: adjust vacuum pump 16 directions, make the glass cylindrical pipe 5 that is connected with sample liquid receiving flask 9 in the face of the operator, the Solid-Phase Extraction passage 14 of solid phase extraction column after the drip washing 7 from the glass cylindrical pipe 5 that is connected with waste collection bottle 6 taken off, be placed on successively on the glass cylindrical pipe 5 that is connected with sample liquid receiving flask 9, the vacuum pump of vacuum pump 16 mouth 12 and the glass cylindrical pipe of corresponding glass cylindrical pipe 5 mouth 8 usefulness of bleeding of bleeding are vacuumized rubber tube 15 closely knit connections, corresponding sample liquid receiving flask 9 is arranged below glass cylindrical pipe 5 successively, in solid phase extraction column 7, add elute soln successively, open vacuum-pump power supply switch 3, control vacuum breaker 2, make elute soln at the uniform velocity flow through solid phase extraction column 7, in sample liquid receiving flask 9, gas clean-up is finished whole Solid-Phase Extraction operation to drain solid phase extraction column 7 in case of necessity.
Claims (5)
1. instant type solid-phase extraction device of online hyperchannel, it is characterized in that, comprise vacuum pump (16), one side surface of vacuum pump (16) is provided with vacuum-pump power supply switch (3), vacuum meter (1), vacuum breaker (2) and the vacuum pump mouth (12) of bleeding, relative two side external surfaces perpendicular to vacuum pump (16), each side external surface is provided with two parallel corrosion resistant plate supports (10), be fixed with semicircle plastics clip (11) on each corrosion resistant plate support (10), be provided with a glass cylindrical pipe (5) between two semicircle plastics clips (11) of each side, glass cylindrical pipe (5) is provided with the glass cylindrical pipe mouth (8) of bleeding, be welded with the Solid-Phase Extraction passage (14) that communicates with glass cylindrical pipe (5) on each glass cylindrical pipe (5), glass cylindrical pipe (5) is stretched out at the two ends of Solid-Phase Extraction passage (14), one end of Solid-Phase Extraction passage (14) is provided with the solid phase extraction column (7) that is used to hold reagent, and interior being connected with solid phase extraction column (7) of Solid-Phase Extraction passage (14) is provided with the glass plug (13) that is used for the reagent circulation.
2. the instant type solid-phase extraction device of online hyperchannel according to claim 1 is characterized in that, the outer surface of cupular part of described vacuum pump (16) is provided with the vacuum pump carrying handle (4) that is used to move.
3. the instant type solid-phase extraction device of online hyperchannel according to claim 1 is characterized in that, described each glass cylindrical pipe (5) is gone up parallel 4-6 the Solid-Phase Extraction passage (14) that be welded with.
4. the instant type solid-phase extraction device of online hyperchannel according to claim 1 is characterized in that, the suitable for reading of described Solid-Phase Extraction passage (14) is the frosted mouth, and end opening is the frosted plug.
5. the instant type solid-phase extraction device of online hyperchannel according to claim 1 is characterized in that, described glass plug (13) is the glass tube of 5mm for internal diameter, and the glass tube end stretches out the outer 15-20mm of Solid-Phase Extraction passage (14) end opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010203018860U CN201589735U (en) | 2010-01-29 | 2010-01-29 | On-line multi-channel immediate solid-phase extraction device |
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| CN2010203018860U CN201589735U (en) | 2010-01-29 | 2010-01-29 | On-line multi-channel immediate solid-phase extraction device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102419353A (en) * | 2011-09-01 | 2012-04-18 | 赛茨科技（大连）有限公司 | Column separation chromatograph |
| CN102662017A (en) * | 2012-05-22 | 2012-09-12 | 天津出入境检验检疫局动植物与食品检测中心 | Pesticide residue detection pretreatment device |
| CN103575571A (en) * | 2012-08-01 | 2014-02-12 | 郭坚 | Multi-channel full-automatic solid-phase extraction device |
-
2010
- 2010-01-29 CN CN2010203018860U patent/CN201589735U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102419353A (en) * | 2011-09-01 | 2012-04-18 | 赛茨科技（大连）有限公司 | Column separation chromatograph |
| CN102662017A (en) * | 2012-05-22 | 2012-09-12 | 天津出入境检验检疫局动植物与食品检测中心 | Pesticide residue detection pretreatment device |
| CN102662017B (en) * | 2012-05-22 | 2014-06-18 | 天津出入境检验检疫局动植物与食品检测中心 | Pesticide residue detection pretreatment device |
| CN103575571A (en) * | 2012-08-01 | 2014-02-12 | 郭坚 | Multi-channel full-automatic solid-phase extraction device |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100922 Termination date: 20130129 |
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| CF01 | Termination of patent right due to non-payment of annual fee |