CN200956029Y - Transferrin colloidalgold test paper - Google Patents
Transferrin colloidalgold test paper Download PDFInfo
- Publication number
- CN200956029Y CN200956029Y CN 200620007249 CN200620007249U CN200956029Y CN 200956029 Y CN200956029 Y CN 200956029Y CN 200620007249 CN200620007249 CN 200620007249 CN 200620007249 U CN200620007249 U CN 200620007249U CN 200956029 Y CN200956029 Y CN 200956029Y
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- antibody
- transferrins
- transferrin
- proxylin
- mental
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Abstract
The utility model relates to a test paper for transferrin colloid detection, which belongs to metal immunity detecting for colloid. The test paper consists of a base board, a bibulous board, a proxylin film, a single anti-mental target cushion of anti-transferrin and a liquid absorbing layer made of glass fabric. The center of base board is made of the proxylin film, on which is provided with a single antibody reaction line for proxylin and a control line for multi-cloning antibody of ovine antibody against mice; on one end of baseboard is provided with a bibulous board, while on the other end is provided with the liquid absorbing layer made of glass fiber; the both ends of proxylin film connects respectively with bibulous board and anti-mental target cushion of anti-tansferrin interactively; the liquid absorbing layer made of glass fiber presses on the anti-mental target cushion of anti-transferrin. When human urine and human waste contains transfferin, the transfferrin antibody can make special combination with mental target probe of anti-transfferin clone body, and there for be identified by anti-transfferrin single clone body, which is called special combination of bi-antibody with filling, so that the content of transfferin in urine and waste can be detected.
Description
Technical field
The present invention relates to a kind of colloid gold immune test paper, it is hemorrhage to judge whether alimentary canal has by the transferrin content in mensuration urine or the ight soil, can help the colorectal cancer early prediction, belongs to the colloid gold immune detection range.
Background technology
The detection of transferrins has very significant values for the diagnosis of hemorrhage of digestive tract.The method of routine inspection hemorrhage of digestive tract now is chemical method and single index immunization.Most hospitals adopt chemical method, this method is of a great variety, but the experimental design principle is basic identical, all being based on hemosiderin in the haemoglobin partly has the effect of catalysis peroxide breakdown, hydrogen peroxide in the energy catalytic reagent, decompose the release new ecological oxygen, the above-mentioned chromogen substance of oxidation and colour generation, what of haemoglobin are the depth of colour generation reacted.Yet,, and lack accuracy and specificity in actual applications because ight soil composition difference is very big, and there is very big-difference in each laboratory concrete operations details difference with the chemical method testing result.The index that single index immunization detects generally is human hemoglobin or human red blood cell matrix, used antibody is antihuman hemoglobin antibody or anti-human erythrocyte matrix antibody, detect human hemoglobin and can detect the hemorrhage of any position of alimentary canal, detect human red blood cell matrix and can only detect hemorrhage of lower digestive tract.But haemoglobin or Rollet poor stability are lost original immunogenicity by digestive ferment degraded sex change or digestion easily; Even secondly haemoglobin or Rollet do not have sex change, but the part-structure change also can influence haemoglobin or Rollet and monoclonal antibody strength of affinity, and then testing result is impacted.Therefore present single index detection is easy to occur upper gastrointestinal bleeding and fails to pinpoint a disease in diagnosis, and false negative phenomenon occurs.
Adopt colloidal gold immunochromatographimethod technology for detection transferrins, overcome the shortcoming of chemical method and single index immunization in the past, a kind of quick, accurate, easy detection method is provided.
Summary of the invention
The present invention has overcome some problems that exist in the prior art, provides a kind of and has pressed from both sides the transferrin content that the antigen method detects urine or ight soil by the collaurum double antibody, can judge whether alimentary canal is hemorrhage, thereby helps the test paper of early stage colorectal cancer screening.
For solving the problems of the technologies described above, the present invention is achieved by the following technical solutions:
Transferrins colloidal gold test base plate middle part is a nitrocellulose filter, an anti-transferrins monoclonal antibody reactive line and a sheep anti mouse polyclonal antibody control line are arranged on the nitrocellulose filter, in base plate one end termination is water sucting plate, other end termination is the glass fiber sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with anti-transferrins monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the glass fiber sample liquid-adsorption layer on anti-transferrins monoclonal antibody gold mark pad.The sampling end of transferrins colloidal gold diagnosis reagent is put into detection sample (liquid level must not surpass the MAX line) because the capillarity test sample will move to the water sucting plate end along test strips, in the sample corresponding transferrins antigen can with anti-transferrins monoclonal antibody gold mark probe generation specific bond after and by anti-transferrins monoclonal antibody identification, the specific bond of double antibodies sandwich promptly takes place, thereby carries out hemorrhage of digestive tract patient's examination by the transferrins that detects in the sample.In the testing process, the gold mark probe that promptly is stranded in this zone when up liquid level will show corresponding redness, and the content of transferrins just is directly proportional in the depth of this response line color and the sample.
When moving to sheep anti mouse polyclonal antibody control line, no matter in the sample transferrin content what, gold mark probe all can combine delay with the sheep anti-mouse igg of bag quilt on the nitrocellulose filter, it is red that control line is shown.So control line does not have, and colour band produces then representative operation wrong (during detection, the sample liquid level surpasses the MAX line) or test paper is expired.
Because adopt technique scheme, transferrins colloidal gold test provided by the present invention has such beneficial effect: i.e. high specificity, highly sensitive, easily store, need not the technical skill personnel operation, do not need instrument and equipment, and readability as a result.
Description of drawings
Fig. 1 is the main TV structure figure of transferrins colloidal gold test.
Fig. 2 is the sectional structure chart of overlooking of Fig. 1.
The positive figure as a result of Fig. 3.
The negative figure as a result of Fig. 4.
1, water sucting plate, 2, nitrocellulose filter, 3, control line, 4, response line, 5, anti-transferrins monoclonal antibody gold mark pad, 6, the glass fiber sample liquid-adsorption layer, 7, base plate, 8, the MAX line
Specific embodiment
Remove external packing during use, take out test strips, test strips sample end is immersed in urine, the ight soil dilution (liquid level must not surpass the MAX line), because the capillarity sample will move to the water sucting plate end along test strips, when having transferrins antigen in urine, the ight soil, in the sample transferrins antigen can with anti-transferrins gold mark probe generation specific bond after and by anti-transferrins monoclonal antibody identification, the specific bond of double antibodies sandwich promptly takes place, thereby carries out hemorrhage of digestive tract patient's examination by the transferrins that detects in the sample.
The result judges: as two red line occurring at detection zone and control zone, then positive when the identical or response line color of color is deeper than control line; It is negative when response line is shallower than control line or a control line only occurs; Red line does not appear in the control zone, shows the expired or misoperation of test paper.
Claims (1)
1. the colloidal gold test of a transferrins, it is characterized in that it is by base plate (7), water sucting plate (1), nitrocellulose filter (2), anti-transferrins monoclonal antibody gold mark pad (5), glass fiber sample liquid-adsorption layer (6) is formed, the base plate middle part is a nitrocellulose filter, a transferrins monoclonal antibody response line (4) and a sheep anti mouse polyclonal antibody control line (3) are arranged on the nitrocellulose filter, in base plate one end termination is water sucting plate, other end termination is the glass fiber sample liquid-adsorption layer, the nitrocellulose filter two ends overlap mutually with anti-transferrins monoclonal antibody gold mark pad with water sucting plate respectively and are connected, and are pressed with the glass fiber sample liquid-adsorption layer on anti-transferrins monoclonal antibody gold mark pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620007249 CN200956029Y (en) | 2006-03-02 | 2006-03-02 | Transferrin colloidalgold test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620007249 CN200956029Y (en) | 2006-03-02 | 2006-03-02 | Transferrin colloidalgold test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN200956029Y true CN200956029Y (en) | 2007-10-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620007249 Expired - Lifetime CN200956029Y (en) | 2006-03-02 | 2006-03-02 | Transferrin colloidalgold test paper |
Country Status (1)
| Country | Link |
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| CN (1) | CN200956029Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965515A (en) * | 2007-10-30 | 2011-02-02 | 约翰·万 | Methods and device for the detection of occult blood |
| CN105021830A (en) * | 2015-07-30 | 2015-11-04 | 中国检验检疫科学研究院 | Double-antibody sandwich colloidal gold detection test strip for human lactoferrin |
| CN105131111A (en) * | 2015-07-30 | 2015-12-09 | 中国检验检疫科学研究院 | Human lactoferrin monoclonal antibody pair |
| CN106199017A (en) * | 2016-09-27 | 2016-12-07 | 重庆大学 | One is occulted blood detection chromatographic test paper |
| CN110261622A (en) * | 2019-07-23 | 2019-09-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample transferrins Test paper and preparation method thereof |
-
2006
- 2006-03-02 CN CN 200620007249 patent/CN200956029Y/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965515A (en) * | 2007-10-30 | 2011-02-02 | 约翰·万 | Methods and device for the detection of occult blood |
| US8053203B2 (en) | 2007-10-30 | 2011-11-08 | John Wan | Methods and device for the detection of occult blood |
| US8349573B2 (en) | 2007-10-30 | 2013-01-08 | John Wan | Methods and device for the detection of occult blood |
| CN105021830A (en) * | 2015-07-30 | 2015-11-04 | 中国检验检疫科学研究院 | Double-antibody sandwich colloidal gold detection test strip for human lactoferrin |
| CN105131111A (en) * | 2015-07-30 | 2015-12-09 | 中国检验检疫科学研究院 | Human lactoferrin monoclonal antibody pair |
| NL2017209A (en) * | 2015-07-30 | 2017-02-02 | Chinese Acad Inspection & Quarantine | Test strip for double-antibody sandwich colloidal golddetection against human lactoferrin |
| CN105131111B (en) * | 2015-07-30 | 2018-03-27 | 中国检验检疫科学研究院 | A kind of human lactoferrin monoclonal antibody pair |
| CN106199017A (en) * | 2016-09-27 | 2016-12-07 | 重庆大学 | One is occulted blood detection chromatographic test paper |
| CN110261622A (en) * | 2019-07-23 | 2019-09-20 | 珠海科域生物工程股份有限公司 | A kind of fecal sample transferrins Test paper and preparation method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20071003 |
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| EXPY | Termination of patent right or utility model |