CN1954666A - Rice protection cytoplasmic male sterile line and recovery line directive breeding method - Google Patents
Rice protection cytoplasmic male sterile line and recovery line directive breeding method Download PDFInfo
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Abstract
A directional breeding method for culturing the cytoplasmic male sterile line of paddy rice includes such steps as hybridizing between the wild rice as female parent and the cultured rice as male parent, choosing the fertile plant as female parent from the hybridized descendant backcrossing with the same cultured rice multiple times, and selfing.
Description
Technical field the present invention relates to sterile line of hybridized rice and recovers the breeding method, particularly protection cytoplasmic male sterile line of system and recover the directive breeding method of system.
The seventies 20th century of background technology, China takes the lead in breeding and promote hybrid rice in the world, obtains immense success.So far, the Three-line Hybrid rice of cytoplasmic male sterile rice assembly accounts for hybrid rice area about 90%, is the main path of rice heterosis utilization.In Three-line Hybrid rice breeding technique system, cytoplasmic male sterile rice breeding and male sterile restoring line breeding are most basic, most important core technologies.About the representative document that cytoplasmic male sterile rice and male sterile restoring line breeding method are discussed has:
1. hybrid rice is learned.The Yuan Longping chief editor, 2002.Chinese agriculture publishing house; 38-127
2. male sterility of rice biology.Zhu Yingguo compiles, 2000.Publishing house of Wuhan University; 1-69; 104-309; 358-425
3. hybrid vigour and hybrid rice breeding.[India] S.S.Virmani work.(Heterosis?andHybrid?Rice?Breeding.
Springer-Verlay?Berlin?Heidelberg，1994.Printed?in?Germany)。Cui Yang Ren, Chen Shunhui translates.1996。Fujian science tech publishing house; 53-104
4. rice breeding is learned.Min Shaokai, Shen Zongtan, Xiong Zhenmin compiles, 1996.Chinese agriculture publishing house; 210-226
5. rice in China kind and pedigree thereof.Lin Shicheng, Min Shaokai compiles, 1991.Shanghai science tech publishing house; 190-196
6. research of hybrid rice and practice.Li ZeBing, volumes such as Xiao Yihua, Zhu Yingguo, nineteen eighty-two.Shanghai science tech publishing house; 32-166
Above document has been narrated the main breeding method of cytoplasmic male sterile rice; Employing has the rice germplasm of male sterile cytoplasm and does maternal, the Yangtze river basin in the cultivated rice early rice variety is made paternal hybrid and backcrosses to breed male sterile line, perhaps do maternal with the cytoplasmic male sterile line of having bred, with the Yangtze river basin in the cultivated rice early between the rice variety offspring's strain of phase mutual cross make paternal hybrid and backcross transformation forms male sterile line, hereditary basis is narrow, and rice quality is poor.Bred the cytoplasm with the indica type male sterile line of paddy rice of production application, concerned classification, mainly can be divided into two types according to extensive guarantor.First type is that to lose (WA) type with the paddy rice open country be the cytoplasm of representative, comprise short losing (DA) type, ridge (G) type, D type, seal short (ID) type, K type, horse association (MX) type etc., the maintenance line of these cytoplasmic male sterile lines is same or similar, and it is also same or similar recovering.Their maintenance line and recovery system can be general mutually, therefore can classify as same or analogous cytoplasm type.Early belong to sporophyte male sterile pollen sterility period of this class cytoplasmic male sterile line on the developmental stage, pollen is based on typical abortion, and sterility is more stable, is the main flow cytoplasm type of using in the hybrid rice production.Second type is the paddy rice HL cytoplasm, and the maintenance line of this cytoplasmic male sterile line is different with wild abortion type, and it is also different with wild abortion type recovering.The pollen abortion of HL cytoplasm male sterile line is later period, and it is sterile to belong to gametophyte on the developmental stage, and pollen runs into megathermal climate and can form a small amount of fertile flower powder based on round abortion, and the fertility stability of male sterile line is relatively poor relatively.
Aspect the main breeding method of recovering to be at rice cytoplasmic male sterile; Above document introduced employing male sterile line test cross select extensive, crossbreeding and radiation breeding between the known recovery system, or the like.These methods can only be applied to carry the breeding of the genetic stocks that recovers gene, and these recovery genes exist only in the late rice variety of low latitudes, hereditary basis is narrow relatively, be difficult to make full use of abundant seed rice resource, especially be difficult to utilize a large amount of colory conventional rice variety resources.Cause a large amount of wastes of hybrid rice breeding resource; Limit the seed selection that high-quality, high yield, high anti-and wide disease and insect resistance, wide adaptability recovery are, limited the further raising of hybrid rice level of aggregation.
The maintenance line of Yebai cytoplasmic male sterile line, main source are confined to rice variety morning of the Yangtze river basin.The rice chalkiness of these maintenance lines is big, the amylose content height, and rice outward appearance and edible quality are poor, have limited the raising of male sterile line and hybrid rice rice quality.For a long time, the rice quality problem of hybrid rice fails to solve well all the time, and its main cause is that the rice quality of male sterile line is good inadequately, and the breeding difficulty is very big.
Common wild-rice (O.rufipogon W.Griffith) is a kind (species) of Oryza (Oryza L.), be distributed widely in South Asia and Southeast Asia, South America, Latin America, Australia, and each provinces and regions such as the Guangdong of SOUTHERN CHINA, Guangxi, Yunnan, Taiwan, Jiangxi, Hainan, Hunan and Fujian.
Cultivate into the existing report of male sterile line with common wild-rice cytoplasm, for example, hybrid rice center, Hunan is done maternal with the wild abortion paddy rice in Hainan, breed the Yebai cytoplasmic male sterile line Zhenshan 97a; Hubei Wuhan University does maternal with the red awns wild rice in Hainan, breed red lotus type cytoplasmic male sterile series Cong Guang 41A; Guangde County, Anhui institute of agricultural sciences does maternal with Jiangxi wild rice of short stem, breed the short type cytoplasmic male sterile line Xieqingzao A that loses, or the like.
Summary of the invention the purpose of this invention is to provide the directive breeding method and the rice protection cytoplasmic male sterile restoring line directive breeding method of rice protection cytoplasmic male sterile line, broken the heredity limitation of wild abortion type male sterile cytoplasm, enlarge the seed selection maintenance line or recover the scope of resource of system, to make full use of abundant seed rice resource as breeding material, the recovery system of male sterile line that the seed selection rice quality is good and high yield and high quality, high anti-wide disease and insect resistance.
One, the directive breeding method of rice protection cytoplasmic male sterile line may further comprise the steps;
1, adopts work female parent not of the same race in the Oryza with protection male sterile cytoplasm; Perhaps do maternal with the common wild-rice of separate sources; Perhaps do kind, strain or the intermediate materials work female parent that female parent is bred with these wild rices; Adopt the cultivated rice kind to make male parent.Not of the same race comprising in the described Oryza with protection male sterile cytoplasm: common wild-rice (O.rufipogon W.Griffith), open up clever wild rice (O.glumaepatula Steud.) Ni Wala wild rice (O.niwara Sharma etShastry), Africa wild rice (O.giaberrima Steud.), Ba Di wild rice (O.barthiiA.Chev.), long stamen wild rice (O.longistaminata A.Chev.et Roehr), south wild rice (O.meridionalis N.Q.Ng), oryza officinalis (O.officinalis Wall.exWatt), oryza meyeriana (O.meyeriana (Zoll et Morrill ex steud.) Baill; The common wild-rice of described separate sources (O.rufipogon W.Griffith) comprising: the Southeast Asia common wild-rice, South America common wild-rice, Latin America common wild-rice, Australian common wild-rice, and the Guangdong common wild-rice of SOUTHERN CHINA, Guangxi common wild-rice, Yunnan common wild-rice, Taiwan common wild-rice, Jiangxi common wild-rice, Hainan common wild-rice, Hunan common wild-rice and Fujian common wild-rice; Described cultivated rice kind comprises: paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, rice leaf hairiness and rice leaf do not have hair (naked body rice).
2, with 1 in the described female parent with described male parent in 1 hybridization, also can be again with 2 or 2 the above male parents in other paternal hybrids.
3, obtain male sterile plant and male fertile plant from filial generation, it is maternal selecting male sterile plant, is male parent with same cultivated rice kind then, continues to backcross.
4, after 3 the method for backcrossing is repeatedly backcrossed set by step, obtain colony's proterties coefficient of variation less than 10%, sterile degree more than 99%, cytoplasmic male sterile line that sterility is stable.Described cytoplasm derives from the female parent that adopts, as wild rice.
5, be that 4 kinds of materials are male parent with the maintenance line of wild abortion type, the maintenance line that recovers system and red lotus type, recovery, maternal with the cytoplasmic male sterile line work of step 4, carry out test cross, gather in the crops the test cross seed respectively; A plantation test cross generation, performance results are the fertility of the cytoplasmic male sterile line that described 4 kinds of materials all can not recovering step 4, and this male sterile line is rice protection cytoplasmic male sterile line.
Two, rice protection cytoplasmic male sterile restoring line directive breeding method may further comprise the steps;
1, adopts work female parent not of the same race in the Oryza with protection male sterile cytoplasm; Perhaps do maternal with the common wild-rice of separate sources; Perhaps do kind, strain or the intermediate materials work female parent that female parent is bred with these wild rices; Adopt the cultivated rice kind to make male parent.Not of the same race comprising in the described Oryza with the male little hatching cell matter of protection: common wild-rice (O.rufipogon W.Griffith), open up clever wild rice (O.glumaepatula Steud.) Ni Wala wild rice (O.niwara Sharma etShastry), Africa wild rice (O.giaberrima Steud.), Ba Di wild rice (O.barthiiA.Chev.), long stamen wild rice (O.longistaminata A.Chev.et Roehr), south wild rice (O.meridionalis N.Q.Ng), oryza officinalis (O.officinalis Wall.exWatt), oryza meyeriana (O.meyeriana (Zoll et Morrill ex steud.) Baill; The common wild-rice of described separate sources (O.rufipogon W.Griffith) comprising: the Southeast Asia common wild-rice, South America common wild-rice, Latin America common wild-rice, Australian common wild-rice, and the Guangdong common wild-rice of SOUTHERN CHINA, Guangxi common wild-rice, Yunnan common wild-rice, Taiwan common wild-rice, Jiangxi common wild-rice, Hainan common wild-rice, Hunan common wild-rice and Fujian common wild-rice; Described cultivated rice kind comprises: paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, rice leaf hairiness and rice leaf do not have hair (naked body rice).
2, with 1 in the described female parent with described male parent in 1 hybridization, also can be again with 2 or 2 the above male parents in other paternal hybrids.
3, obtain male sterile plant and male fertile plant from filial generation, it is maternal selecting male fertile plant, is male parent with same cultivated rice kind then, continues to backcross.
4, after 3 the method for backcrossing is repeatedly backcrossed set by step, obtain the economical character progeny material that normally can educate similar to male parent;
5, the progeny material selfing is isolated sterile strain and can educate strain, select the fertile plant selfing to separate again, neatly stable up to proterties, the coefficient of variation is less than 10%; Select economical character neat similar in appearance to male parent, colony, fertility normally can be educated strain more than 80%, is the rice protection cytoplasmic male sterile restoring line.
Rice protection cytoplasmic male sterile line of the present invention and protection cytoplasmic male sterile restoring line directive breeding method, be characterized in maintenance line and recover the wide material sources of system, breed excellent sterile system and recover system probability improve, many improved seeds comprise maintenance line, recovery system and the red lotus type maintenance line of wild abortion type and recover system, conventional variety, can breed the protection male sterile line or recover system, the level of aggregation of breeding protection male sterile line or recovery system increases substantially.The present invention obtains novel protection male sterile cytoplasm, abbreviates FA cytoplasm as.
The characteristics of protection FA male sterile line of the present invention are;
1. the wild abortion type (WA) of protection FA cytoplasm and production extensive use and the cytoplasmic extensive guarantor of red lotus type (HL) concern differently, are a kind of novel cytoplasm.As shown in table 1;
2. the maintenance line of wild abortion type with maintenance line that recovers system, red lotus type and recovery system all can not be as the cytoplasmic recovery of protection FA, and can only be as the cytoplasmic maintenance line of protection FA.As shown in table 1;
Table 1, the performance of peasants who dig gold's 1 male sterile line test cross first generation of hybrid fertility
3. the maintenance line wide material sources of protection cytoplasmic, maintenance line is bred the level of aggregation height of male sterile line to seed rice resource utilization height.
The advantage of protection FA male sterile line of the present invention is; 1. the maintenance line of protection FA male sterile line source is many.2. the fertility stability of protection FA male sterile line is good.3. the rice quality of protection FA male sterile line is excellent.As shown in table 2;
The main pluses and minuses of table 2 three class cytoplasmic male sterile rices relatively
| Cytoplasm type | The maintenance line source | Sterile stability | Rice quality |
| Zhenshan 97a (WA) | Few | Good | Difference |
| Cong Guang 41A (HL) | Few | Difference | In |
| Peasants who dig gold 1A (FA) | Many | Good | Excellent |
Rice protection cytoplasmic male sterile restoring line directive breeding method of the present invention, with common wild-rice and good cultivated rice inter breed crossing with backcross, through offspring's isolation and selection, orientation is bred protection and is recovered system.Be characterized in;
1. the fertility that efficient recovery protection male sterile line can be arranged makes the protection male sterile line realize three series mating and production application.
2. the most extensive and utilized the resource potential of existing seed rice fully, the recovery assemblage Heshui of breeding is flat high, and the hybrid rice of assembly can great-leap-forward ground raising rice quality and output.
Rice protection cytoplasmic male sterile restoring line directive breeding method of the present invention.The advantage that the protection of breeding recovers system and hybrid rice is; 1. protection recovery system and the hybrid rice production stability bred are good.2. the protection of breeding recovers system and hybrid rice wide adaptability.3. the protection of breeding recovers system and hybrid rice output height.4. protection recovery system and the hybrid rice rice quality bred are excellent.
Embodiment is illustrated below in conjunction with embodiment in order fully to disclose rice protection cytoplasmic male sterile line of the present invention and to recover the directive breeding method of system.
Embodiment one: the directive breeding method of rice protection cytoplasmic male sterile line
1, be maternal with the Fujian common wild-rice, it is that be paternal hybrid morning extensive 89 that wild abortion type recovers, and to select male sterile plant from filial generation be maternal, be male parent with morning extensive 89 then, continue repeatedly to backcross, generally backcross and breed male sterile line peasants who dig gold 1A after 5 times, with described test cross method, show complete sterility through the test cross offspring, can confirm as and belong to protection cytoplasmic male sterile line.This male sterile line economical character is good.12 indexs of protection male sterile line peasants who dig gold 1A rice quality all reach the superior rice standard that the Ministry of Agriculture issues.Wherein 1 grade of superior rice standard is 8,4 of 2 grades of superior rice standards.
2, do maternal with protection cytoplasmic male sterile line peasants who dig gold 1A, win safe No. 1 with good cultivated rice kind and make paternal hybrid, and to select male sterile plant from filial generation be maternal, be male parent with winning safe No. 1 then, continue repeatedly to backcross, generally backcross and breed protection cytoplasmic male sterile line peasants who dig gold 2A after 5 times, economical character is good.12 indexs of protection male sterile line peasants who dig gold 2A rice quality all reach the superior rice standard that the Ministry of Agriculture issues.Wherein 1 grade of superior rice standard is 6,6 of 2 grades of superior rice standards.
3, do maternal with protection cytoplasmic male sterile line peasants who dig gold 1A, with grain quality rice kind ruby be paternal hybrid, and to select male sterile plant from filial generation be maternal, be male parent with ruby then, continue repeatedly to backcross, generally backcross and breed protection cytoplasmic male sterile line peasants who dig gold 3A after 5 times, have the leaf attitude of good strain, economical character and yield traits and different friendship proterties.12 indexs of peasants who dig gold 3A rice quality all reach the superior rice standard that the Ministry of Agriculture issues.Wherein 1 grade of superior rice standard is 6,6 of 2 grades of superior rice standards.
Embodiment two: rice protection cytoplasmic male sterile restoring line directive breeding method
1, it is maternal to use the Fujian common wild-rice to do, and hybridizes with rice varieties special blue or green 63, and it is maternal selecting male fertile plant from filial generation, is paternal hybrid with special blue or green 63 then, and selecting male fertile plant again from filial generation is female parent, hybridizes for male parent with special blue or green 63 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain repeatedly selfing again from the self progeny, and can obtain after the general selfing 2 times that protection that colony is entirely male fertile plant recovers is FR1.Peasants who dig gold 1A is that the FR1 combo becomes hybrid rice combination peasants who dig gold 1A/FR1, the performance of the plantation first generation of hybrid with recovery; Fringe is big, grain is many, ripening rate is high, output is high.Peasants who dig gold 1A/FR1 hybrid rice rice quality the key technical indexes reaches country's " high quality paddy " grade III Standard.
2, recovering with protection is that FR1 makes female parent, and with bright extensive 63 hybridization of rice varieties, it is maternal selecting male fertile plant from filial generation, is paternal hybrid with bright extensive 63 then, and it is maternal selecting male fertile plant again from filial generation, hybridizes for male parent with bright extensive 63 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain repeatedly selfing again from the self progeny, and can obtain after the general selfing 2 times that protection that colony is entirely male fertile plant recovers is FR2.Peasants who dig gold 2A is that the FR2 combo becomes hybrid rice combination peasants who dig gold 2A/FR2 with recovery.Plantation first generation of hybrid performance fringe is big, grain is many, ripening rate is high, output is high.Peasants who dig gold 2A/FR2 hybrid rice rice quality the key technical indexes reaches country's " high quality paddy " grade III Standard.
3, recovering with protection is that FR1 makes female parent, and with bright extensive 70 hybridization of rice varieties, it is maternal selecting male fertile plant from filial generation, is paternal hybrid with bright extensive 70 then, and it is maternal selecting male fertile plant again from filial generation, hybridizes for male parent with bright extensive 70 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain repeatedly selfing again from the self progeny, and can obtain after the general selfing 2 times that protection that colony is entirely male fertile plant recovers is FR3.Is that the FR3 combo becomes hybrid rice combination peasants who dig gold 3A/FR3 with peasants who dig gold 3A with recovery, and plantation first generation of hybrid performance fringe is big, grain is many, ripening rate is high, output is high.Peasants who dig gold 3A/FR3 hybrid rice rice quality the key technical indexes reaches country's " high quality paddy " grade III Standard.
Claims (8)
1, the directive breeding method of rice protection cytoplasmic male sterile line is characterized in that may further comprise the steps;
(1) adopts work female parent not of the same race in the Oryza with protection male sterile cytoplasm; Perhaps do maternal with the common wild-rice of separate sources; Perhaps do kind, strain or the intermediate materials work female parent that female parent is bred with these wild rices; Adopt the cultivated rice kind to make male parent; Not of the same race comprising in the described Oryza with protection male sterile cytoplasm: common wild-rice (O.rufipogonW.Griffith), open up clever wild rice (O.glumaepatula Steud.) Ni Wala wild rice (O.niwara Sharma et Shastry), Africa wild rice (O.giaberrima Steud.), Ba Di wild rice (O.barthii A.Chev.), long stamen wild rice (O.longistaminataA.Chev.et Roehr), south wild rice (O.meridionalis N.Q.Ng), oryza officinalis (O.officinalis Wall.ex Watt), oryza meyeriana (O.meyeriana (Zoll etMorrill ex steud.) Baill; The common wild-rice of described separate sources (O.rufipogonW.Griffith) comprising: the Southeast Asia common wild-rice, South America common wild-rice, Latin America common wild-rice, Australian common wild-rice, and the Guangdong common wild-rice of SOUTHERN CHINA, Guangxi common wild-rice, Yunnan common wild-rice, Taiwan common wild-rice, Jiangxi common wild-rice, Hainan common wild-rice, Hunan common wild-rice and Fujian common wild-rice; Described cultivated rice kind comprises: paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, rice leaf hairiness and rice leaf do not have hair (naked body rice);
(2) with 1 in the described female parent with described male parent in 1 hybridization, also can be again with 2 or 2 the above male parents in other paternal hybrids;
(3) obtain male sterile plant and male fertile plant from filial generation, it is maternal selecting male sterile plant, is male parent with same cultivated rice kind then, continues to backcross;
(4) set by step after the method for backcrossing of (3) is repeatedly backcrossed, obtain colony's proterties coefficient of variation less than 10%, sterile degree more than 99%, cytoplasmic male sterile line that sterility is stable;
(5) be that 4 kinds of materials are male parent with the maintenance line of wild abortion type, the maintenance line that recovers system and red lotus type, recovery, maternal with the cytoplasmic male sterile line work of step (4), carry out test cross, gather in the crops the test cross seed respectively; A plantation test cross generation, performance results are the fertility of the cytoplasmic male sterile line that described 4 kinds of materials all can not recovering step (4), and this male sterile line is rice protection cytoplasmic male sterile line.
2, the directive breeding method of rice protection cytoplasmic male sterile line according to claim 1, it is characterized in that with the Fujian common wild-rice being maternal, it is that be paternal hybrid morning extensive 89 that wild abortion type recovers, and to select male sterile plant from filial generation be maternal, be male parent with morning extensive 89 then, breed male sterile line peasants who dig gold 1A after continuing repeatedly to backcross, with described test cross method, the offspring shows complete sterility through test cross.
3, the directive breeding method of rice protection cytoplasmic male sterile line according to claim 1, it is characterized in that: do maternal with protection cytoplasmic male sterile line peasants who dig gold 1A, win safe No. 1 with good cultivated rice kind and make paternal hybrid, and to select male sterile plant from filial generation be maternal, be male parent with winning safe No. 1 then, breed protection cytoplasmic male sterile line peasants who dig gold 2A after continuing repeatedly to backcross.
4, the directive breeding method of rice protection cytoplasmic male sterile line according to claim 1, it is characterized in that: do maternal with protection cytoplasmic male sterile line peasants who dig gold 1A, with grain quality rice kind ruby be paternal hybrid, and to select male sterile plant from filial generation be maternal, be male parent with ruby then, breed protection cytoplasmic male sterile line peasants who dig gold 3A after continuing repeatedly to backcross.
5, rice protection cytoplasmic male sterile restoring line directive breeding method is characterized in that may further comprise the steps:
(1) adopts work female parent not of the same race in the Oryza with protection male sterile cytoplasm; Perhaps do maternal with the common wild-rice of separate sources; Perhaps do kind, strain or the intermediate materials work female parent that female parent is bred with these wild rices; Adopt the cultivated rice kind to make male parent; Not of the same race comprising in the described Oryza with protection male sterile cytoplasm: common wild-rice (O.rufipogonW.Griffith), open up clever wild rice (O.glumaepatula Steud.) Ni Wala wild rice (O.niwara Sharma et Shastry), Africa wild rice (O.giaberrima Steud.), Ba Di wild rice (O.barthii A.Chev.), long stamen wild rice (O.longistaminataA.Chev.et Roehr), south wild rice (O.meridionalis N.Q.Ng), oryza officinalis (O.officinalis Wall.ex Watt), oryza meyeriana (O.meyeriana (Zoll etMorrill ex steud.) Baill; The common wild-rice of described separate sources (O.rufipogonW.Griffith) comprising: the Southeast Asia common wild-rice, South America common wild-rice, Latin America common wild-rice, Australian common wild-rice, and the Guangdong common wild-rice of SOUTHERN CHINA, Guangxi common wild-rice, Yunnan common wild-rice, Taiwan common wild-rice, Jiangxi common wild-rice, Hainan common wild-rice, Hunan common wild-rice and Fujian common wild-rice; Described cultivated rice kind comprises: paddy rice, upland rice, long-grained nonglutinous rice, japonica rice, early rice, late rice, rice leaf hairiness and rice leaf do not have hair (naked body rice);
(2) with 1 in the described female parent with described male parent in 1 hybridization, also can be again with 2 or 2 the above male parents in other paternal hybrids;
(3) obtain male sterile plant and male fertile plant from filial generation, it is maternal selecting male fertile plant, is male parent with same cultivated rice kind then, continues to backcross;
(4) set by step after the method for backcrossing of (3) is repeatedly backcrossed, obtain the economical character progeny material that normally can educate similar to male parent;
(5) the progeny material selfing is isolated sterile strain and can educate strain, select the fertile plant selfing to separate again, neatly stable up to proterties, the coefficient of variation is less than 10%; Select economical character neat similar in appearance to male parent, colony, fertility normally can be educated strain more than 80%, is the rice protection cytoplasmic male sterile restoring line.
6, rice protection cytoplasmic male sterile restoring line directive breeding method according to claim 5, it is characterized in that: do maternal with the Fujian common wild-rice, with special blue or green 63 hybridization of rice varieties, it is maternal selecting male fertile plant from filial generation, be paternal hybrid with special blue or green 63 then, it is maternal selecting male fertile plant again from filial generation, hybridizes for male parent with special blue or green 63 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain more repeatedly after the selfing from the self progeny, and can obtain that protection that colony is entirely male fertile plant recovers is FR1.
7, rice protection cytoplasmic male sterile restoring line directive breeding method according to claim 5, it is characterized in that: recovering with protection is that FR1 makes female parent, with bright extensive 63 hybridization of rice varieties, it is maternal selecting male fertile plant from filial generation, be paternal hybrid with bright extensive 63 then, it is maternal selecting male fertile plant again from filial generation, hybridizes for male parent with bright extensive 63 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain can to obtain repeatedly that protection that colony is entirely male fertile plant recovers after the selfing be FR2 again from the self progeny.
8, rice protection cytoplasmic male sterile restoring line directive breeding method according to claim 5, it is characterized in that: recovering with protection is that FR1 makes female parent, with bright extensive 70 hybridization of rice varieties, it is maternal selecting male fertile plant from filial generation, be paternal hybrid with bright extensive 70 then, it is maternal selecting male fertile plant again from filial generation, hybridizes for male parent with bright extensive 70 again; So repeatedly backcross, generally backcross after 5 times, choosing can be educated the strain selfing, and choosing can be educated strain more repeatedly after the selfing from the self progeny, and can obtain that protection that colony is entirely male fertile plant recovers is FR3.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835303A (en) * | 2012-09-14 | 2012-12-26 | 湖南杂交水稻研究中心 | Breeding method of polima genic-cytoplasmic male sterile line |
| CN103329796A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source |
| CN106900536A (en) * | 2015-12-22 | 2017-06-30 | 中国种子集团有限公司 | Photo-thermo sensitive male nuclear sterile rice line orients transformation into the method for protection cytoplasmic male sterile line |
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2005
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835303A (en) * | 2012-09-14 | 2012-12-26 | 湖南杂交水稻研究中心 | Breeding method of polima genic-cytoplasmic male sterile line |
| CN103329796A (en) * | 2013-07-11 | 2013-10-02 | 江西省农业科学院水稻研究所 | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source |
| CN103329796B (en) * | 2013-07-11 | 2016-03-02 | 江西省农业科学院水稻研究所 | The universal method of paddy rice cytoplasmic male sterilty restorer is cultivated in a kind of slewing |
| CN106900536A (en) * | 2015-12-22 | 2017-06-30 | 中国种子集团有限公司 | Photo-thermo sensitive male nuclear sterile rice line orients transformation into the method for protection cytoplasmic male sterile line |
| CN106900536B (en) * | 2015-12-22 | 2022-03-08 | 中国种子集团有限公司 | Method for directionally transforming rice photo-thermo sensitive genic male sterile line into wide-range cytoplasmic male sterile line |
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