CN1554448A - Chitosan gelatine polyvinyl alcohol biological hemostatic dressing - Google Patents
Chitosan gelatine polyvinyl alcohol biological hemostatic dressing Download PDFInfo
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- CN1554448A CN1554448A CNA200310121182XA CN200310121182A CN1554448A CN 1554448 A CN1554448 A CN 1554448A CN A200310121182X A CNA200310121182X A CN A200310121182XA CN 200310121182 A CN200310121182 A CN 200310121182A CN 1554448 A CN1554448 A CN 1554448A
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- polyvinyl alcohol
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Abstract
The biological chitosan-gelatin-polyvinyl alcohol hemostatic dressing is compounded with chitosan, gelatin, polyvinyl alcohol and glycerin, and is prepared into hemostatic cotton, spray and other externally applied medicine forms via conventional production process. The present invention has high adsorption amount, maintained amount, no toxicity, easy absorption by body and high hemostatic function and is superior to available hemostatic dressing.
Description
Technical field:
The present invention relates to the chitosan is the improvement of main material production bleeding-stopping dressing, has further provided the biological bleeding-stopping dressing of a kind of chitosan gelatin polyvinyl alcohol, belongs to medical hemostatic dressing
Technical field.
Background technology
Chitosan (chiosail.cs) is also referred to as chitosan, is the main derivant of chitin, and its preparation is simple, the source is abundant, low price, is nontoxic micromolecular compound glucosamine by biodegradation easily, therefore more and more is subject to people's attention.Developing with chitin and chitosan in recent years in succession is the bleeding-stopping dressing of Main Ingredients and Appearance, materials such as many employing gelfoam, also having with chitin, gelatin is the hemostatic material of Main Ingredients and Appearance, but these materials are desirable not enough what have aspect hydrophilic, film property, tractive and the haemostatic effect.Report as the hemostatic material document is existing with chitosan and gelatin merely, but,, in hemostatic material, only account for about 2%, thereby influenced haemostatic effect, and elasticity is too little, is inconvenient to use because the dissolubility of chitosan is bad because its anastalsis is limited.
Summary of the invention:
The invention provides the biological bleeding-stopping dressing of a kind of chitosan gelatin polyvinyl alcohol, overcome the shortcoming of existing bleeding-stopping dressing aspect hydrophilic, film property, tractive and haemostatic effect, kept original hemostatic function again.
The present invention is made by following raw materials by weight portion ratio:
5～60 parts of chitosans, 5～40 parts in gelatin, 5～60 parts of polyvinyl alcohol, 5～40 parts of glycerol.
Optimum ratio of the present invention is (by weight):
40 parts of chitosans, 35 parts in gelatin, 40 parts of polyvinyl alcohol, 20 parts of glycerol.
Concrete technology is as follows:
At first chitosan is dissolved in the acetic acid solution of 0.2～2% concentration; It is molten respectively polyvinyl alcohol to be added hydro-thermal again, the gelatin heating for dissolving, and glycerol is dissolved in water, and above-mentioned dissolved various solution are mixed, fully stir to make evenly, lyophilization, the cutting packing, sterilization makes product of the present invention.
Utilize bleeding-stopping dressing of the present invention to produce topical administration dosage forms such as styptic cotton, spray by conventional external used medicine dosage form manufacturing technique.
The character of utilizing the hemostasis of chitosan, antibacterial, antibiotic property, biocompatibility, promotion wound healing among the present invention and being easy to form gel cooperates polyvinyl alcohol nontoxic non-stimulated, has extremely strong hydrophilic and fabulous film property.
Gelatin is that the Main Ingredients and Appearance of composition tissue is the main matter that forms the sponge state, thereby improves the elasticity of this product, and for wound provides nutrition, promotes wound healing, prevents that cicatrix from generating.Glycerol plays humidification in this product, makes sthptic sponge easier to be fused with tissue.
Good effect of the present invention is: utilize the mutual compatibility of chitosan, gelatin, polyvinyl alcohol and glycerol, blood had high adsorbance, reservation amount, nontoxic, be easy to be absorbed by body, solved that original biological hemostasis adjuvant hygroscopicity is poor, the shortcoming of weak frangible, the haemostatic effect difference of pulling force.
Below test example is proof result of use of the present invention further:
1, materials and methods
1.1 material
Chitosan is purchased in Qingdao
Gelatin is purchased in Shanghai
Polyvinyl alcohol is purchased in Shanghai
Glycerol is purchased in Shanghai
1.2 method
1) hemostatic material: chitosan, polyvinyl alcohol are dissolved respectively, and glycerol is dissolved in the water, and above three kinds of solution are mixed, and stirs to make evenly, and lyophilization makes biological sthptic sponge.
2) physical property:
For the physical property of hemostatic material, can represent that its meaning and unit are as follows with apparent density, wetting time, absorbtivity, absorbance:
The weight of apparent density (ρ) expression sponge material under unit volume, the mg/cm of unit
3
The every gram sponge material saturated with water of wetting time (t) expression is divided required time, the t/g of unit
The every gram sponge material of water absorption (m) expression absorbs the gram number of moisture, unit water/gram
Water absorption rate (ω) representation unit is in the time, the gram number of every gram moisture that sponge material absorbs, the g of unit water/gram-second
3) haemostatic effect
This experiment is carried out preliminary study to the anthemorrhagic performance and the reliability of biological hemostatic material, and with the chitosan gelfoam in contrast.Used animal is the 2.5-3.0kg large ear rabbit, and male and female are not limit.Laboratory sample is through Co
60The hemorrhage wound surface of 2cm * 1cm size is made in radiation sterilization in rabbit ear portion.20% urethane is anaesthetized rabbit through auricular vein under aseptic condition, the stomach wall preserved skin, and routine disinfection, the medisection stomach wall enters the abdominal cavity.Form the hemorrhage wound surface of oozing of blood at liver, spleen skin cut 1cm * 1cm tissue, apply with autonomous biological hemostasis adjuvant and chitosan gelatin hemostatic material respectively and press hemostasis, the record bleeding stopping period, the bonding situation of observing adjuvant and wound surface is in the hope of the healing state of routine observation wound surface.
4) vivo degradation experiment
Under the aseptic condition, 20% urethane is anaesthetized rabbit (about 5ml/kg) through auricular vein, the stomach wall preserved skin, and routine disinfection, the medisection stomach wall enters the abdominal cavity.Form oozing of blood, hemorrhage wound surface at liver, spleen skin cut 1cm * 0.5cm tissue, after the definite hemostasis of the deposited pressure of hemostatic material, hemostatic material is stayed in the organ, close the conventional raising in abdominal cavity then, put to death animal in 15 days, 30 days, 60 days respectively at postoperative, get liver, spleen is done the pathology inspection, matched group uses the chitosan gelfoam.
2, experimental result
2.1 the physical property of biological sthptic sponge and chitin absorbable gelatin sponge.
The physical property test result of sthptic sponge of the present invention and carapace ammonia absorbable gelatin sponge: table 1
Hemostatic material sthptic sponge chitosan of the present invention gelfoam
1??????????2???????????3?????????????1????????????2???????????3
Density
14.0???????14.32???????13.99?????????13.1?????????13.0????????13.0
mg/m
3
Wetting time
1.0×10
4??1.02×10
4?1.01×10
4????3.35×10
4??3.21×10
4??3.4×10
4
t/g
Water absorption
43?????????42??????????43????????????30???????????31??????????30
gH
2O/v
2.2 haemostatic effect is observed: (table 2)
| Hemostatic material | The example number | Average bleeding stopping period | Hemostasis percentage rate (%) | ||
| Ear | Liver | Spleen | |||
| The present invention's silk floss that stops blooding | ????20 | ????58±4 | ????60±6 | ????62±8 | ??100 |
| Carapace ammonia gelfoam | ????20 | ????60±6 | ????64±13 | ????65±10 | ??100 |
In the table 2, the continuous bleeding stopping period of biological hemostasis is shorter than the chitosan absorbable gelatin sponge, and experiment the present invention continuous wound surface ability that adheres to of stopping blooding is better than the chitosan sthptic sponge.
2) hemostatic reliability
On 20 hemorrhage wound surface of rabbit ear portion making, observed in continuous back 10 minutes, 24 hours, 48 hours with the present invention's tender carapace ammonia gelatin hemostasis of stopping blooding respectively, there is no that hemostatic material comes off and bleeding again.10 livers, spleen wound surface, hemostatic material apply presses hemostasis to observe in back 10 minutes to there is no hemorrhage and oozing phenomenon.Sponge and wound surface adhere to good.Observed result was the same in 24 hours, 48 hours.Hemostatic material and sludged blood form a complete covering on wound surface, see that wound surface has the mauve fibrous membrane of one deck, does not have bleeding again after removing this covering.
3) healing state of wound surface and vivo degradation absorbing state
Hepar Leporis seu Oryctolagi, spleen stopped blooding back 15 days, all observed the partial hemostasis covering of wound surface for two groups and had formed the machine scar tissue, and omentum majus tissue and its adhesion are arranged.After 30 days, the present invention's continuous material that stops blooding partly is absorbed, the local fiber spot that forms of wound surface after 60 days, and hemostatic material all is absorbed.The matched group situation is similar substantially to experimental group, but the local formation of wound surface local organization cicatrix is bigger than experimental group after 60 days.
3, discuss
From above experiment as can be seen, sea of blood silk floss of the present invention has good hygroscopicity, and the short more performance of tissue intersolubility and hemostasis compares to similar product, and not only traction force strengthens convenient the use, and effect is better than reference substance.
The specific embodiment:
The following example is intended to further describe for example the present invention, rather than limit the present invention by any way, under the prerequisite that does not deviate from the spirit and principles in the present invention, any change or change that those of ordinary skills that the present invention did are realized easily all will fall within the claim scope that awaits the reply of the present invention.
Embodiment 1
Chitosan 20g is dissolved in the acetic acid solution of 600ml 0.2% concentration, gets polyvinyl alcohol 20g and add boiling water and make dissolving, gelatin 15g heating is dissolved, glycerol 15g is dissolved in water, and merges above-mentioned solution, fully stirs to make evenly, lyophilization, the cutting packing, the Co60 sterilization makes bleeding-stopping dressing.
Embodiment 2
Chitosan 40g is dissolved in the acetic acid solution of 1000ml 1.2% concentration, gets polyvinyl alcohol 55g and add boiling water and make dissolving, gelatin 30g heating is dissolved, glycerol 40g is dissolved in water, and merges above-mentioned solution, fully stirs to make evenly, lyophilization, the cutting packing, sterilization makes bleeding-stopping dressing.
Embodiment 3
Chitosan 60g is dissolved in the acetic acid solution of 800ml 0.8% concentration, polyvinyl alcohol 40g adds boiling water makes dissolving, and gelatin 10g heating is dissolved, glycerol 10g is dissolved in water, and merges above-mentioned solution, fully stirs to make evenly, lyophilization, the cutting packing, the Co60 sterilization makes bleeding-stopping dressing.
Embodiment 4
Embodiment 1～3 is made bleeding-stopping dressing be adsorbed on equably that to make biological hemostasis on the cotton wool continuous.
Embodiment 5
Embodiment 1～3 is made an amount of powder of waving of bleeding-stopping dressing adding make spray.
Claims (4)
1, the biological bleeding-stopping dressing of a kind of chitosan gelatin polyvinyl alcohol is characterized in that it being to be made by following raw materials by weight portion ratio:
5～60 parts of chitosans, 5～40 parts in gelatin, 5～60 parts of polyvinyl alcohol, 5～40 parts of glycerol.
2, bleeding-stopping dressing according to claim 1 is characterized in that being made by following raw materials by weight portion ratio:
40 parts of chitosans, 35 parts in gelatin, 40 parts of polyvinyl alcohol, 20 parts of glycerol.
3, the production method of the biological bleeding-stopping dressing of a kind of chitosan gelatin polyvinyl alcohol, it is characterized in that: at first chitosan is dissolved in the acetic acid solution of 0.2～2% concentration, it is molten respectively polyvinyl alcohol to be added hydro-thermal again, the gelatin heating is dissolved, glycerol is dissolved in water, above-mentioned dissolved various solution are mixed, and fully stirring makes evenly, and sterilization makes product of the present invention.
4, biological bleeding-stopping dressing according to claim 1 and 2 is produced topical administration dosage forms such as styptic cotton, spray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200310121182XA CN1554448A (en) | 2003-12-23 | 2003-12-23 | Chitosan gelatine polyvinyl alcohol biological hemostatic dressing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200310121182XA CN1554448A (en) | 2003-12-23 | 2003-12-23 | Chitosan gelatine polyvinyl alcohol biological hemostatic dressing |
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| Publication Number | Publication Date |
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| CN1554448A true CN1554448A (en) | 2004-12-15 |
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| CNA200310121182XA Pending CN1554448A (en) | 2003-12-23 | 2003-12-23 | Chitosan gelatine polyvinyl alcohol biological hemostatic dressing |
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327849C (en) * | 2005-03-05 | 2007-07-25 | 汕头市金丰医疗器械科技有限公司 | Hemostatic agent and its preparing method |
| CN100348272C (en) * | 2006-06-23 | 2007-11-14 | 华南理工大学 | Method for preparing astringent sponge of soluble cellulose |
| CN101879324A (en) * | 2010-07-02 | 2010-11-10 | 西南大学 | Method for preparing chitosan-based composite dressing for medical use |
| CN101912634A (en) * | 2010-08-06 | 2010-12-15 | 华南理工大学 | Method for preparing nanosilver/nano silicon dioxide-containing antibacterial biological dressing |
| CN101927029A (en) * | 2010-08-06 | 2010-12-29 | 华南理工大学 | Preparation method of chitosan/polyvinyl alcohol sponge dressing containing nano-silver |
| CN102108138B (en) * | 2009-12-28 | 2012-07-25 | 沈阳鑫盛生物科技有限公司 | Method for preparing chitosan collagen gel |
| CN102671232A (en) * | 2012-05-18 | 2012-09-19 | 成都理工大学 | Tourmaline/chitosan hydrochloride compound spray-type aqueous dressing and preparation method thereof |
| CN103007339A (en) * | 2012-12-06 | 2013-04-03 | 浙江医鼎医用敷料有限公司 | Biological powder medical dressing and preparation method thereof |
| CN103239753A (en) * | 2013-04-28 | 2013-08-14 | 华南理工大学 | Hydroxypropyl-beta-cyclodextrin/chitosan medical dressing and preparation method thereof |
| CN103316377A (en) * | 2013-06-27 | 2013-09-25 | 华南理工大学 | Beta-cyclodextrin/carboxymethyl chitosan medical dressing and preparation method thereof |
| CN103705968A (en) * | 2013-12-30 | 2014-04-09 | 深圳先进技术研究院 | Medical chitosan compound moisturizing dressing and preparation method thereof |
| CN103736135A (en) * | 2013-12-12 | 2014-04-23 | 深圳先进技术研究院 | Medicinal composite dressing and preparation method thereof |
| CN104524624A (en) * | 2014-12-17 | 2015-04-22 | 安徽省健源医疗器械设备有限公司 | Chitosan hemostatic gauze and preparation method thereof |
| WO2015187103A1 (en) * | 2014-05-21 | 2015-12-10 | Punyanitya Sittiporn | Surgical hemostatic based rice starch |
| US9283187B2 (en) | 2011-04-27 | 2016-03-15 | Biom'up | Hemostatic compositions |
| CN106344080A (en) * | 2016-08-30 | 2017-01-25 | 宗兆文 | Hemostasis device for closed abdominal cavity bleeding |
| CN107469138A (en) * | 2017-10-09 | 2017-12-15 | 常州金艺广告传媒有限公司 | A kind of preparation method of high imbibition promoting healing type compound hemostatic material |
| WO2020021499A1 (en) * | 2018-07-26 | 2020-01-30 | Azista Industries Pvt Ltd | Haemostatic gel composition and its process of preparation |
| RU2720627C1 (en) * | 2019-09-09 | 2020-05-12 | Общество с ограниченной ответственностью "Химико-фармацевтические технологии" (ООО "ХимФармТех") | Haemostatic composition with reparative properties (versions) |
| US11052172B2 (en) | 2016-08-12 | 2021-07-06 | Biom'up France SAS | Hemostatic flowable |
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2003
- 2003-12-23 CN CNA200310121182XA patent/CN1554448A/en active Pending
Cited By (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327849C (en) * | 2005-03-05 | 2007-07-25 | 汕头市金丰医疗器械科技有限公司 | Hemostatic agent and its preparing method |
| CN100348272C (en) * | 2006-06-23 | 2007-11-14 | 华南理工大学 | Method for preparing astringent sponge of soluble cellulose |
| CN102108138B (en) * | 2009-12-28 | 2012-07-25 | 沈阳鑫盛生物科技有限公司 | Method for preparing chitosan collagen gel |
| CN101879324A (en) * | 2010-07-02 | 2010-11-10 | 西南大学 | Method for preparing chitosan-based composite dressing for medical use |
| CN101927029B (en) * | 2010-08-06 | 2013-06-12 | 华南理工大学 | Preparation method of chitosan/polyvinyl alcohol sponge dressing containing nano-silver |
| CN101912634A (en) * | 2010-08-06 | 2010-12-15 | 华南理工大学 | Method for preparing nanosilver/nano silicon dioxide-containing antibacterial biological dressing |
| CN101927029A (en) * | 2010-08-06 | 2010-12-29 | 华南理工大学 | Preparation method of chitosan/polyvinyl alcohol sponge dressing containing nano-silver |
| CN101912634B (en) * | 2010-08-06 | 2013-09-25 | 华南理工大学 | Method for preparing nanosilver/nano silicon dioxide-containing antibacterial biological dressing |
| US10342856B2 (en) | 2011-04-27 | 2019-07-09 | Biom'up | Hemostatic compostions |
| US10046034B2 (en) | 2011-04-27 | 2018-08-14 | Biom'up | Hemostatic compositions |
| US9662374B2 (en) | 2011-04-27 | 2017-05-30 | Biom'up | Hemostatic compositions |
| US9283187B2 (en) | 2011-04-27 | 2016-03-15 | Biom'up | Hemostatic compositions |
| CN102671232B (en) * | 2012-05-18 | 2014-08-13 | 成都理工大学 | Tourmaline/chitosan hydrochloride compound spray-type aqueous dressing and preparation method thereof |
| CN102671232A (en) * | 2012-05-18 | 2012-09-19 | 成都理工大学 | Tourmaline/chitosan hydrochloride compound spray-type aqueous dressing and preparation method thereof |
| CN103007339A (en) * | 2012-12-06 | 2013-04-03 | 浙江医鼎医用敷料有限公司 | Biological powder medical dressing and preparation method thereof |
| CN103007339B (en) * | 2012-12-06 | 2014-11-26 | 浙江医鼎医用敷料有限公司 | Biological powder medical dressing and preparation method thereof |
| CN103239753A (en) * | 2013-04-28 | 2013-08-14 | 华南理工大学 | Hydroxypropyl-beta-cyclodextrin/chitosan medical dressing and preparation method thereof |
| CN103316377A (en) * | 2013-06-27 | 2013-09-25 | 华南理工大学 | Beta-cyclodextrin/carboxymethyl chitosan medical dressing and preparation method thereof |
| CN103736135B (en) * | 2013-12-12 | 2015-11-04 | 深圳先进技术研究院 | A kind of Medical composite dressing and preparation method thereof |
| CN103736135A (en) * | 2013-12-12 | 2014-04-23 | 深圳先进技术研究院 | Medicinal composite dressing and preparation method thereof |
| CN103705968A (en) * | 2013-12-30 | 2014-04-09 | 深圳先进技术研究院 | Medical chitosan compound moisturizing dressing and preparation method thereof |
| CN103705968B (en) * | 2013-12-30 | 2015-10-28 | 深圳先进技术研究院 | A kind of medical chitosan compound moisture-preserving dressing and preparation method thereof |
| WO2015187103A1 (en) * | 2014-05-21 | 2015-12-10 | Punyanitya Sittiporn | Surgical hemostatic based rice starch |
| CN104524624A (en) * | 2014-12-17 | 2015-04-22 | 安徽省健源医疗器械设备有限公司 | Chitosan hemostatic gauze and preparation method thereof |
| US11052172B2 (en) | 2016-08-12 | 2021-07-06 | Biom'up France SAS | Hemostatic flowable |
| CN106344080A (en) * | 2016-08-30 | 2017-01-25 | 宗兆文 | Hemostasis device for closed abdominal cavity bleeding |
| CN107469138A (en) * | 2017-10-09 | 2017-12-15 | 常州金艺广告传媒有限公司 | A kind of preparation method of high imbibition promoting healing type compound hemostatic material |
| WO2020021499A1 (en) * | 2018-07-26 | 2020-01-30 | Azista Industries Pvt Ltd | Haemostatic gel composition and its process of preparation |
| RU2720627C1 (en) * | 2019-09-09 | 2020-05-12 | Общество с ограниченной ответственностью "Химико-фармацевтические технологии" (ООО "ХимФармТех") | Haemostatic composition with reparative properties (versions) |
| WO2021049978A1 (en) * | 2019-09-09 | 2021-03-18 | Limited Liability Company "Khimiko-Farmatsevticheskie Tekhnologii" | Hemostatic composition with reparative properties (variants) |
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