CN1548133A - Chinese medicine composition with spleen-invigorating and mass-eliminating effect - Google Patents
Chinese medicine composition with spleen-invigorating and mass-eliminating effect Download PDFInfo
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Abstract
The Chinese medicine composition is prepared with white atractylodes rhizome, immature bitter orange, magnolia bark, haw, radish seed and fingered citron. During its preparation, the materials are processed through distillation, decoction and ethanol extraction to obtain the effective components. The component identification and content measurement method is also provided to control the quality of the Chinese medicine composition. The Chinese medicine composition has excellent effect of invigorating spleen and eliminating mass and is suitable for children.
Description
Invention field
The present invention relates to a kind of Chinese medicine composition, particularly have the Chinese medicine composition of spleen invigorating removing food stagnancy effect, relate to the preparation method and the method for quality control of said composition simultaneously.
Background technology
" stagnating " is motherland's medical science name of disease, and clinical symptoms shows as anorexia, and the abdominal part distension is eaten without digesting, belching and acid regurgitation, and stool acid is smelly etc.Ancient Chinese medicine doctor thinks that all it is the stagnant important pathogenesis of formation that insufficiency of the spleen mistake fortune, breast food are stagnated.Stagnant is more common a kind of gastrointestinal tract disease in children's's period, and accumulating in the course of time does not disappear, and alimentation and the growth promoter of controlling the normal children's of influence lost in delay.Say as " A complete work on Pediatrics, a collection and arrangement of the chief contents from ancient pediatrics, written by Chen Fuzheng ": " get ill from overeating-demonstrate,prove, close terriblely most, as delay not rule of treatment Cheng Jicheng addiction; That controls improperly then becomes infantile malnutrition to become consumptive disease." therefore pay attention to the control of infantile malnutrition, significant to guaranteeing growing up healthy and sound of hundreds of millions children.
Modern medicine is thought: the digestion of food, absorption are two kinds of processes of different nature, and digestion refers to that food decomposes at digestive tract, absorb to refer to that food sees through intestinal wall through its nutrient substance after digesting and enters blood circulation.Food digestion is more abundant, and absorption of nutrient ingredients is more complete, and absorption process suitably slows down, and nutrient substance stops in digestive tract, also helps gastrointestinal peristalsis and digestive juice secretion, promotes the further digestion of food.Therefore digesting and absorb two kinds of processes complements each other, mutually promotes.Normal stool road motor function is finished by gastrointestinal smooth muscle, gastrointestinal smooth muscle keeps certain tension force and wriggling is to grind food, propelling movement chyme, the necessary condition of drainage waste matter, and smooth muscle is suitably lax, the control intestine evacuation velocity, plays a part then to guarantee that intestinal wall blood fully pours into microcirculation, improves mucosa nutritive, improves barrier function, prevents to damage, suppresses the enteral fermentation, the growth of favourable normal flora, promotes absorption function.Stagnant infant stops enteral because dyspepsia does not disappear, and causes the aggravation of carbohydrate fermentation and protein decay process.The bacterial fermentation saccharide generates multiple gases such as lactic acid and carbon dioxide; Protein acts on through bacillus septicus, though aerogenesis is few, decomposites a large amount of noxious substances, as phenol, indole, methylindole, hydrogen sulfide and various amines etc.When the gas flaring intestinal wall, poisonous substance is absorbed Shi Zeke and suppresses gastrointestinal motility and secretory function, and therefore gastrointestinal dysfunction syndromes such as belching and acid regurgitation, abdominal distension and anorexia, stool are uncomfortable, thick fur rolling pulse appear in the sick child.
Summary of the invention
One object of the present invention is to disclose a kind of new Chinese medicine composition with the effect of spleen invigorating removing food stagnancy; Another object of the present invention is a kind of new spleen invigorating removing food stagnancy method of drug composition on that has of open preparation; The object of the invention also is to disclose a kind of method of quality control of new Chinese medicine composition.The crude drug of pharmaceutical composition of the present invention is formed and proportioning following (by weight):
Rhizoma Atractylodis Macrocephalae 220-280 weight portion Fructus Aurantii Immaturus 220-280 weight portion Cortex Magnoliae Officinalis 150-220 weight portion
Fructus Crataegi 220-280 weight portion Semen Raphani 220-280 weight portion Fructus Citri Sarcodactylis 150-220 weight portion
The preferred Fructus Crataegi (parched to brown) of described Fructus Crataegi, the preferred Fructus Aurantii Immaturus (parched) of Fructus Aurantii Immaturus, the preferred Sichuan Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) of Cortex Magnoliae Officinalis, the preferred Semen Raphani (parched) of Semen Raphani.
This preparation of drug combination method:
Get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 8-12 times of water gaging, warm macerating 1-3 hour, distill and extracted volatile oil in 1-3 hour, collect volatile oil and add the betacyclodextrin that 3-5 doubly measures and make clathrate with polishing, cold drying is standby below 60-30 ℃, gained water liquid filters standby, medicinal residues add 5-7 times of water gaging again and decocted 20-40 minute, filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adding 8-12 times of water gaging decocted 0.5-2 hour, filter, medicinal residues add 6-8 times of water gaging again and decocted 20-40 minute, filter, decocting liquid such as the filtrate and the Rhizoma Atractylodis Macrocephalae merges, be concentrated into 40-60 ℃ of relative density 1.0-1.15, add ethanol and make and contain alcohol amount and reach 50-65%, cold preservation 20-30 hour, filter, the clear paste that it is 1.30-1.45 that filtrate recycling ethanol, water liquid continue to be concentrated into 50-70 ℃ of relative density, volatile oil clathrate compounds such as the qinghuo reagent and the above-mentioned Rhizoma Atractylodis Macrocephalae, through conventional operation directly or add pharmaceutically acceptable excipient and make clinical acceptable forms, as tablet, oral liquid, capsule, granule etc.
The method of quality control that this compositions is made medicament comprises discriminating and/or assay.
Discrimination method comprises a kind of and/or several in the following method:
A. get this composite preparation 6g, add water 50ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, adds water from determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 1-2 hour, divide and get the ethyl acetate layer, as need testing solution, other gets Rhizoma Atractylodis Macrocephalae control medicinal material 1g, 60-90 ℃ adds petroleum ether 15ml, 50-70 ℃ of water-bath warm macerating 1 hour, filtration, evaporate to dryness, residue add ethyl acetate 1ml dissolving, as Rhizoma Atractylodis Macrocephalae control medicinal material solution; Get Fructus Citri Sarcodactylis control medicinal material 1g again, add dehydrated alcohol 10ml, supersound process 8-15 minute, filter, filtrate is concentrated into about 1ml, as Fructus Citri Sarcodactylis control medicinal material solution; Test according to thin layer chromatography (2000 editions appendix VIB of Chinese Pharmacopoeia), drawing each 3--6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, is developing solvent with petroleum ether 60-90 ℃-ethyl acetate 3-5: 1-2, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Fructus Citri Sarcodactylis control medicinal material chromatograph on, show identical navy blue speckle; Spray is with 10% ethanol solution of sulfuric acid, and 100-115 ℃ was dried by the fire 2-7 minute, and put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Rhizoma Atractylodis Macrocephalae control medicinal material chromatograph on, show the fluorescence speckle of same color;
B. get Cortex Magnoliae Officinalis control medicinal material 10g, add water 100ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 2-4 hour, divide and get the ethyl acetate layer, as Cortex Magnoliae Officinalis control medicinal material solution; Test according to thin layer chromatography (2000 editions appendix VIB of Chinese Pharmacopoeia), draw each 5 μ l of need testing solution and above-mentioned control medicinal material solution, put respectively on same silica gel g thin-layer plate, so that petroleum ether (60-90 ℃)--ethyl acetate 8-12: 1-2 is developing solvent, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;
Assay: get this composite preparation fine powder 15g, the accurate title, decide, and puts in the conical flask, precision adds methanol 50ml, close plug, and it is fixed accurately to claim, supersound process 30-50 minute, put coldly, add methanol and supply weight, filter, discard filtrate just, precision is measured subsequent filtrate 35ml, put and be concentrated into about 5ml in the water-bath, add water 10ml and stir moltenly, transfer pH to 1-2 with 2% hydrochloric acid, with extracted with diethyl ether 3 times, each 20ml discards ether solution, water liquid is transferred pH to 9-10 with ammonia, extracts 5 times with the ethyl acetate jolting, each 20ml, merge ethyl acetate liquid, put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, move on the alumina column of having handled well, with methanol 100ml eluting, eluent is put evaporate to dryness in the water-bath, and residue adds methanol makes dissolving in right amount, move in the 5ml measuring bottle, add methanol to scale, shake up, as need testing solution; Other gets the Neosynephrine reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution; According to thin layer chromatography (2000 editions one appendix VI B of Chinese Pharmacopoeia) test, draw need testing solution 4 μ l, reference substance solution 1 μ l and 4 μ l, the cross point is on same silica gel g thin-layer plate respectively, and (3-5: 1-2: 4-6) upper strata liquid is developing solvent, saturated 15-25 minute with n-butyl alcohol-glacial acetic acid-water, launch, take out, cold wind dries up, and spray is with 0.5% ethanol solution of ninhydrin, 90-110 ℃ was dried by the fire about 8-12 minute, take out, on cover a same big or small glass plate, seal with adhesive plaster all around; Scan wavelength X according to thin layer chromatography (2000 editions one appendix VI B of Chinese Pharmacopoeia thin layer chromatography scanning)
s=510nm, λ
R=700nm measures test sample trap integrated value and reference substance trap integrated value, calculates, that is, this composite preparation per unit amount contains Fructus Aurantii Immaturus with Neosynephrine (C
9H
13NO
2), must not be less than 0.80mg.
Above-mentioned unit quantity is meant the finished medicines dosage of suitable 11g crude drug.
The present composition has the effect of good spleen invigorating removing food stagnancy, can promote the gastric secretion function, and gastric secretion, free acidity, total acidity, total acid output and pepsin activity are obviously increased.
Following experimental example is used to further specify the present invention.
Experimental example 1
Pharmacodynamic experiment
One, experiment material:
(1), medicine
1, the thick extractum of this composite preparation (spleen invigorating removing food stagnancy): Guizhou greatly patented technology Applied Research Laboratory provides lot number: 970106.Contain crude drug amount 1g/ml medicinal liquid with preceding being diluted to.The each 10ml of the clinical children's's consumption of this product, every day 3 times, daily dose 30ml amounts to crude drug amount 30g/d, and children's presses average 20Kg weighing machine, by formula is converted to people's consumption per day and is:
Adult's consumption is 1.75g/Kg/d.20Kg child consumption is 1.5/Kg/d.For the ease of adjusting the dosage and the capacity of animal ig administration, face the time spent is made into desired concn with distilled water medicinal liquid.
2, XIANGSHA YANGWEI WAN: Zaozhuang pharmaceutical factory of traditional Chinese medicine produces, lot number: 960203, and face the time spent and make 15% medicinal liquid with the distilled water grinding.
3, children's indigestion tablet: the Jinan pharmaceutical factory of traditional Chinese medicine produces, lot number: 961102.Face the time spent and make 5% medicinal liquid with the distilled water grinding.
4, reserpine injection: red flag pharmaceutical factory of Shanghai Medical Univ produces, lot number: 951105; All the other are national reagent, analytical pure.
(2) animal: 1, Kunming kind white mice and Wistar rat, all available from medical animal experiment center, Shandong Province, the quality certification number: Shandong kinoplaszm: 960101.2, rabbit is available from medical animal experiment center, Shandong Province.
(3) instrument: 1, DC-001 type isolated organ analyzer Nanjing analytical tool factory produces.2,501 type ultrathermostat Shanghai, second hardware factory produces.3,722 type grating spectrophotometer Shanghai Shen instrument automatic control company produces.4,800 type centrifugation device Shanghai operating theater instruments ten factories produce.
Two, method and result:
(1) to the stomach function influence 1, to the influence of white mice gastric emptying: get 60 of body weight 20-22 gram white mice, the male and female dual-purpose, fasting is 12 hours before the experiment, freely drinks water.Be divided into 5 groups at random, every group 12, first three groups is the medicinal liquid 40,20 of the thick extractum preparation of ig spleen invigorating removing food stagnancy and 10g/Kg (for estimating 24,12,6 times of clinical consumption) respectively, positive controls ig15% XIANGSHA YANGWEI WAN 6g/Kg (be clinical consumption 20 times), the distilled water 0.4ml/10g of capacity such as blank group ig.Behind the administration 50min, each group is ig0.1% methyl orange solution 0.1ml/10g respectively, and white mice is put to death in the 20min dislocation, cut open the belly and win stomach and place in the small beaker, add people 10ml distilled water, cut off stomach along greater gastric curvature with little shears, gastric content is fully washed in distilled water 5%NaHCO
3Solution is regulated pH value to 6.0-6.5, pours graduated centrifuge tube into, from 10min, gets supernatant with 722 type grating spectrophotometers with 2000rpm, returns to zero with distilled water, and the optical density of measuring solution is a methyl orange optical density in the stomach.Getting 0.1% methyl orange 0.2ml again adds people 10ml distilled water and shakes up the back and measure its optical density as radix methyl orange optical density.And by following formula calculating methyl orange stomach residual rate.
The results are shown in Table 1.
Table 1 spleen invigorating removing food stagnancy granule is to the influence of white mice gastric emptying (X ± SD)
Group dosage number of animals methyl orange stomach residual rate
(g/Kg) (only) (%)
Matched group-12 32.6 * 3.75
10 12 42.0×5.86**
Spleen invigorating removing food stagnancy extractum 20 12 53.3 * 5.21**
40 12 64.9+5.35**
XIANGSHA YANGWEI WAN 6 12 49.4 ± 4.56**
Annotate: compare with the distilled water group: * shows P＜0.05, and * * shows P＜0.01 (same following table)
By table 1 as seen, each administration group all can obviously improve methyl orange stomach residual rate, with matched group significant statistical significance is arranged relatively; Experiment shows: spleen invigorating removing food stagnancy granule has the mice of slowing down gastric emptying.
2, influence to rat gastric secretion function: get 60 of body weight 250-270 gram rat, the male and female dual-purpose is divided into five groups at random, every group 12, the first three groups medicinal liquid 20,10 and the 5g/Kg of the thick extractum preparation of ig spleen invigorating removing food stagnancy respectively (is 12 of the clinical consumption of expectation, 6 and 3 times), positive controls ig5% children's indigestion tablet 1g/Kg (be clinical consumption 10 times), the distilled water of capacity such as blank group ig, administration capacity 2ml/100g, be administered once every day, continuous 5 days, after the last administration, fasting water supply 24h under etherization, cuts off osculum along ventrimeson, find out stomach gently, the ligation door again by duodenal administration once, is sewed up incision of abdominal wall, the fu jie of taking out stitches out behind the 5h is pricked pylorus, extract full stomach, cut off coat of the stomach, collect gastric juice, measure the gastric juice amount respectively, free acid, total acidity, total acid output, pepsin activity.Acidity assaying adopts holder to take indicator and two step of phenolphthalein titrimetry, and pepsin activity adopts Mai Te (mett) capillary glass-tube method, the results are shown in Table 2.
Table 2 spleen invigorating removing food stagnancy granule is to the influence of rat gastric secretion function (X ± SD)
The total acid output pepsin activity of group number of animals dosage gastric juice amount free acidity total acidity
(only) be (ml/5h) (mmol/L) (mmol/L) (mmol/L) (mm) (g/kg)
Matched group 12-8.3 ± 1.12 51.8 ± 5.65 83.9 ± 16.1 0.71 ± 0.14 4.26 ± 0.51
Spleen invigorating removing food stagnancy 12 5 10.7 ± 1.67** 65.3 ± 12.4* 98.3 ± 17.2* 1.04 ± 0.19** 5.48 ± 1.52*
Extractum 12 10 12.5 ± 1.38** 76.5 ± 8.20** 115.8 ± 12.8** 1.48 ± 0.18** 5.95 ± 10.69**
12 20 14.4±2.72** 90.8±14.2** 131.2±19.7** 1.86±0.33** 6.60±1.34**
Children's indigestion tablet 12 1 13.8 ± 1.95** 88.2 ± 14.9** 126.3 ± 18.9** 1.62 ± 0.38** 6.20 ± 0.69**
As can be seen from Table 2, each group of administration can make rat gastric secretion, free acidity, total acidity, total acid output and pepsin activity obviously increase, and compares with matched group, and significant statistical significance is arranged; The result shows: this granule can significantly strengthen rat gastric secretion function.
(2) to the influence of intestinal function
1. to the influence of mouse small intestine propelling rate: get fasting water supply 24h, 60 of body weight 18-22 gram white mice, the male and female dual-purpose, be divided into 5 groups at random, every group 12, first three groups is the medicinal liquid 40,20 of the thick extractum preparation of ig spleen invigorating removing food stagnancy and 10g/Kg (for estimating 24,12,6 times of clinical consumption) respectively, positive controls ig15% XIANGSHA YANGWEI WAN 6g/Kg (be clinical consumption 20 times), the distilled water 0.4ml/10g of capacity such as blank group ig.50min after the administration, 5 groups of mices are ig charcoal end normal saline suspension 0.2ml/10g respectively, takes off cervical vertebra behind the 20min with sacrifice of animal, cuts open the belly immediately, press the literature method operation, calculating intestinal propulsion percentage rate.
The results are shown in Table 3.
Table 3 spleen invigorating removing food stagnancy granule is to the influence of mouse small intestine propelling rate (X ± SD)
Group dosage (g/Kg) number of animals (only) intestinal propulsion percentage rate (%)
Matched group-12 73.4 ± 12.9
10 12 59.3±11.3**
Spleen invigorating removing food stagnancy extractum 20 12 45.6 ± 6.80**
40 12 37.1±7.80**
XIANGSHA YANGWEI WAN 6 12 54.4 ± 8.60**
The result shows: each medication group intestinal propulsion percentage rate is starkly lower than matched group, the equal highly significant of difference.Illustrate that this medicine can obviously suppress the ahead running of white mice small intestinal.
2, to the influence at body rabbit intestinal smooth muscle: get 40 of body weight 2-2.5Kg healthy rabbits, the male and female dual-purpose is divided into five groups at random, 8 every group.Tie up on operating-table with 25% urethane 1g/Kg intravenous anesthesia layback position, the abdominal part cropping is along stomach wall center hunter's line otch, open the abdominal cavity, pull out one section in ileum, respectively to wear a short-term at a distance of the 5cm interval, knotting is fixing and stay the end of a thread standby on the same plane of myenteron.Centre at two short-terms, seam one long line, then long line is lain on the differential transformer hook through suitable for reading the pulling out of myenteron stationary pipes, with DC-001 type isolated organ analyzer record myenteron shrinkage curve, as control value before the medicine, then, each is organized ig respectively and gives the medicinal liquid 10,7 of the thick extractum preparation of spleen invigorating removing food stagnancy and 3.5g/Kg (for estimating 6,4,2 times of clinical consumption), positive controls gives 15% XIANGSHA YANGWEI WAN 1.5g/Kg (for 5 times of clinical consumption), the blank group waits the distilled water of capacity, administration capacity 10ml/Kg.30min myenteron shrinkage curve the results are shown in Table 4 before and after the record.
Table 4 spleen invigorating removing food stagnancy granule is at the influence of body rabbit intestinal smooth muscle (X ± SD)
Group dosage (g/Kg) number of animals (only) intestinal tube muscular tension reduces percentage rate (%)
Matched group-8 3.2 ± 1.45
3.5 8 33.1±5.43**
Spleen invigorating removing food stagnancy extractum 78 52.4 ± 4.10**
10 8 76.6±11.2**
XIANGSHA YANGWEI WAN 1.5 8 53.6 ± 12.1**
By table 4 as seen, each administration group intestinal tube muscular tension obviously reduces, and compares with matched group, and significant statistical significance is arranged, and experimental result shows: spleen invigorating removing food stagnancy granule can make the muscular tension at body rabbit intestinal smooth muscle obviously reduce.
3, to the exsomatize influence of ileum spasm of rabbit due to acetylcholine, the barium chloride: get the rabbit of the about 2Kg of body weight, the male and female dual-purpose, it is deadly that auricular vein injects air, cuts open the belly and fetch intestinal, and about every section 2cm, it is standby to put into tyrode's solution.Get one section of intestinal tube, put into the Magnus' bath that fills the 30ml tyrode's solution, 38 ± 0.5 ℃ of water temperatures.When intestinal tube lies in differential generation spasm, add the medicinal liquid and the 15% XIANGSHA YANGWEI WAN medicinal liquid 1ml of the thick extractum preparation of 100%, 50%, 25% spleen invigorating removing food stagnancy respectively, matched group adds the 1ml distilled water.Experimental result, each medication group all has antagonism to the spastic contraction of ileum of exsomatizing of rabbit due to acetylcholine and the barium chloride, the results are shown in Table 5.
Table 5 spleen invigorating removing food stagnancy granule is to the influence of rabbit ileum spasm due to acetylcholine, the barium chloride (X ± SD)
Dosage antagonism percentage rate (%)
The group experiment number
(g) acetylcholine barium chloride
Matched group 10-4.4 ± 1.2 5.3 ± 1.8
10 0.25 62.8±9.60** 58.4±12.32**
Spleen invigorating removing food stagnancy extractum 10 0.50 88.3 ± 14.2** 81.9 ± 20.3**
10 1.00 107.4±19.2** 98.6±17.4**
XIANGSHA YANGWEI WAN 10 0.15 72.6 ± 17.4** 64.2 ± 8.6**
By table 5 as seen, each administration group has obvious antagonism to the spastic contraction of rabbit ileum due to acetylcholine and the barium chloride, and along with dosage increasing effect is strengthened, with the equal highly significant of matched group comparing difference.The result shows: this granule has tangible spasmolysis.
(3) reserpine is caused the fatigue proof influence of " insufficiency of the spleen " white mice: get 72 of body weight 20-22 gram white mice, the male and female dual-purpose is divided into 6 groups at random, 12 every group.(1) reserpine model group: every day 1 subcutaneous injection of reserpine 0.3mg/Kg, continuous 14 days, 8-14 days every days ig distilled water 0.4ml/10g
(2) reserpine adds spleen invigorating removing food stagnancy groups of grains: it is the same to cause " insufficiency of the spleen ", 8-14 days, the medicinal liquid 40,20 of the thick extractum preparation of the removing food stagnancy of ig spleen invigorating respectively and 10g/Kg (for estimating 24,12,6 times of clinical consumption) are organized in 3 treatments, once a day, and administration capacity 0.4ml/10g.
(3) reserpine adds the XIANGSHA YANGWEI WAN group: it is the same to cause " insufficiency of the spleen ".8-14 days, ig15% XIANGSHA YANGWEI WAN 6g/Kg (be clinical consumption 20 times), once a day, administration capacity 0.4ml/10g
(4) normal control group: every day 1 subcutaneous injection normal saline 0.1ml/10g, continuous 14 days, 8-14 days every days ig distilled water 0.4ml/10g.
After the last administration one hour, one by one with the weight of a white mice body weight 10% of white mice afterbody bundle, put people's depth of water 20cm, swimming in the glass jar that water temperature is 20 ± 0.5 ℃, observe the mouse head submerged and can not the person of emerging be muscle power exhaustion in 10 seconds, at once timing is the swimming time of white mice, the results are shown in Table 6.
Table 6 spleen invigorating removing food stagnancy granule causes the fatigue proof influence of " insufficiency of the spleen " white mice (X ± SD) to reserpine
Group dosage (g/Kg) number of animals (only) swimming continuance time (min)
Normal control group-12 54.2 ± 11.4
Reserpine model group-12 25.9 ± 4.3 △ △
Reserpine adds spleen invigorating 10 12 34.6 ± 6.4*
Removing food stagnancy extractum group 20 12 37.2 ± 4.8**
40 12 41.8±10.5**
Reserpine adds XIANGSHA YANGWEI WAN 6 12 39.4 ± 7.4**
Reserpine model group and normal control group are than △ △ P＜0.01.Reserpine dosing group with the flat model group of blood than * P＜0.05, * * P＜0.01.
As can be seen from Table 6, the reserpine model group obviously shortens than normal control group white mice swimming continuance time, illustrates that reserpine can cause white mice " insufficiency of the spleen " model.Model dosing group obviously prolongs than model control group swimming time, carries out test of significance, significant difference.
Experiment shows: spleen invigorating removing food stagnancy granule improves significantly to white mice " insufficiency of the spleen " symptom due to the reserpine.
Three, conclusion:
1, functional study shows to stomach: the spleen invigorating removing food stagnancy granule white mice gastric emptying that can slow down, strengthen rat gastric secretion function, illustrate that this medicine can obviously strengthen the function of holding and hoarding food of stomach, thereby alleviate food back feeling of repletion, for the digestion that increases gastric secretion provides the time, improve the function of gastric digestion food.
2, intestinal function be studies show that: this granule suppresses the ahead running of white mice small intestinal, is reduced in the tension force of body rabbit intestinal smooth muscle, illustrates that this medicine energy food and extend in the time that small intestinal stops, makes food be able to abundant absorption in raising.Rabbit due to acetylcholine and the barium chloride exsomatized back raise spastic contraction, this granule has antagonism, points out this medicine to remove spasm by the blocking-up m receptor, to the intestinal tube smooth muscle direct repression be arranged also, and this is with to treat the weakness of the spleen and stomach parasympathetic nervous high partially relevant.
3, reserpine is caused " insufficiency of the spleen ".White mice endurance experimentation shows: this granule clothes liquid can prolong the swimming time of " insufficiency of the spleen " white mice, this and treatment weakness of the spleen and stomach, and lassitude and weak is relevant.
In sum, spleen invigorating removing food stagnancy granule is with to treat diseases such as infantile spleen deficiency dyspepsia clinically consistent, so this composite preparation curative effect is reliable.
Experimental example 2This composite preparation toxicological study
One, acute toxinology experiment: give the thick extractum of this composite preparation of mouse stomach (infant spleen-tonifying removing food stagnancy) (amounting to crude drug amount 400g/kg/d), observed continuously seven days, mice generally in order, none death.This dosage is equivalent to 228 times of clinical 60kg people's per kilogram of body weight consumption per day, and this product safety and low toxicity are described.
Two, long-term toxicological test: give the thick extractum of rat oral gavage infant spleen-tonifying removing food stagnancy (amounting to crude drug amount 100g/kg/d, 50g/kg/d), be equivalent to 60 and 30 times of the clinical crude drug consumption per day of 60kg people, in successive administration 1 month and drug withdrawal in the time of 15 days, observe the organ coefficient and the histopathologic change of the biochemical and important organ of general situation, hematology, urine, the blood of animal, all no abnormal, illustrate that clinical medicine dose and administration time that this product is worked out are safe and reliable.
Experimental example 3Preparation technology preferably studies
One, medicine extraction process such as Rhizoma Atractylodis Macrocephalae is preferably studied
1, decoction pieces granularity: extract volatile oil and should reduce particle diameter as far as possible, be beneficial to the stripping of oil, but meticulous easy burnt the paste to increase surface area, therefore with 10-20 order granule for well, 60 order fine powders no more than 20%.
2, be that the index orthogonal optimum seeking is fried in shallow oil the condition of putting forward with the volatile oil yield:, get four Chinese medicine coarse granule Rhizoma Atractylodis Macrocephalae 40g, Fructus Aurantii Immaturus 40g in the prescription ratio, Fructus Citri Sarcodactylis 30g, Cortex Magnoliae Officinalis 30g, totally nine parts, respectively put in the 2000ml round-bottomed flask, on connect volatile oil extractor, decoct by condition under each row item of orthogonal experiment, collection volatile oil (wall adhesion person wash with ether) moves in the little evaporating dish of constant weight, convulsion is flung to ether in the kitchen, weigh with torsion balance, deduct evaporating dish weight, get volatile oil weight.With the filtrate standardize solution, each precision is measured 10ml, drop on the 3g polyamide fine powder, evaporate to dryness in the water-bath, move to (60-80 order, diameter 10mm) on the 1g polyamide column is housed, use 70% ethanol elution, the collection eluent to scale, is measured trap (representing the content of flavone component) in the 283nm place in the 50ml measuring bottle.
Table 7 oil extracting process is preferred
A. amount of water (doubly) B. dip time (h) (h) D. volatile oil trap when C. fries in shallow oil
Weight (g) A
6 mercerations 0.5 1.0
8 mercerations 2.0 2.0
10 warm macerating 2.0 4.0
1 1 1 1 1 0.321 0.285
2 1 2 2 2 0.385 0.367
3 1 3 3 3 0.407 0.406
4 2 1 2 3 0.366 0.406
5 2 2 3 1 0.387 0.486
6 2 3 1 2 0.393 0.430
7 3 1 3 2 0.412 0.402
8 3 2 1 3 0.402 0.408
9 3 3 2 1 0.455 0.472
I 1.113 1.088 1.116
II 1.135 1.174 1.195
III 1.269 1.245 1.206
R 0.156 0.157 0.090
I 1.058 1.095 1.123
II 1.323 1.260 1.247
III 1.282 1.308 1.293
R 0.255 0.213 0.046
The result shows, by intuitive analysis, and No. 9 test A
3B
3C
2Be optimal conditions, analyze that A, B two factors influence bigger to the result, select excellent horizontal A by extreme difference R
3B
3, C is a secondary cause, angle should be selected C from saving time
2, because A
3B
3Be worth greatlyyer, append two tests again, (12 times of water B
3C
2) (A
3, 70-80 ℃ of warm macerating 3h, C
2).Volatile oil weight is 0.455g, and 0.462g is with A
3B
3Difference is little, based on the volatile oil yield, takes into account flavones ingredient simultaneously, and saves time, and considers with the big production angle of industry, adds 10 times of water gagings, 70-80 ℃ of warm macerating 2h, and distillation 2h is an optimum condition.
3, two investigations of frying in shallow oil condition: four Chinese medicines such as the Rhizoma Atractylodis Macrocephalae are after above-mentioned warm macerating distillation, and surplus medicinal residues need decoct once more and could guarantee that extracts active ingredients is complete, from saving time and the consideration of cost angle, through one fry in shallow oil after, two fry in shallow oil and add 6 times of water gagings again and fry in shallow oil 0.5h and get final product.
4, owing to first fry in shallow oil warm macerating 9h, distill 2h again, decoct through secondary, with two boil medicine slag again heat once see Table 3 the 3rd and fry in shallow oil, cream 0.360g only, two friedly reach 94%, needn't fry in shallow oil repeatedly.
Two, Fructus Crataegi, Semen Raphani decocting process are investigated:
With organic acid in the Fructus Crataegi is index, investigated decocting condition with orthogonal test, according to document (observation that organic acid content changes in the arteries and veins peace electuary. Wu Ershen. Chinese patent medicine 1992, (10): 8. in the Fructus Crataegi organic acid for the first time fried total amount 75%, secondary is fried to reach 90%), designed the secondary decocting condition.
Analyze from extreme difference R, amount of water is a principal element, promptly adds 10 times of water gagings, and two fry in shallow oil and add 7 times of water gagings; And the decocting time result influences not quite, investigate from the big production angle that saves time, and be excellent to fry in shallow oil 1h, 0.5h respectively.
Three, precipitate with ethanol and not heavy influence to finished product dosage
Relative density being reached 1.08 medicinal liquid is divided into two, portion not precipitate with ethanol is handled, a handle to make by the technology precipitate with ethanol contain the alcohol amount and reach 60%, all be concentrated into 1.36 clear paste, add adjuvant system soft material system granule in 1: 2.5: 0.5 ratio, should adorn 19g (and spissated easy paste, finished product has cinder, it is qualified that the melting test is difficult for) without the sample of precipitate with ethanol through converting a bag, and the purified sample of precipitate with ethanol only is 8g, concerning children's's preparation, discard the dross and select the essential, it is more rational reducing taking dose.
Four. the screening of system soft material system granule adjuvant ratio
Water liquid one behind the recovery ethanol is divided into four, continues to be concentrated into 1.301,1.340,1.365,1.391 (60=heat is surveyed), add adjuvant system soft material, the required adjuvant of the former two (needing about 5 times, 4 times respectively just can disperse) bigger than normal, the latter two are soft suitable; But the sample of relative density 1.391, because thickness is difficult for being uniformly dispersed with adjuvant; Clear paste with relative density 1.365 is more suitable, determines that therefore the clear paste relative density is 1.35-1.38 (60 ℃ of heat is surveyed).Press clear paste-sugar part-dextrin (1: 2: 0.5,1: 2.5: 0.5,1: 3: 1) and make soft material respectively, the former adjuvant is on the low side, sticking net, with 1: 2.5: 0.5 more suitable, the adjuvant ratio is lower.For making the finished particle uniformity, heavy-gravity clear paste adds an amount of ethanol and stirs evenly dispersion, makes the mobile enhancing of clear paste, adds adjuvant again and disperses, so the easy uniformity of finished particle.
Following embodiment all can realize the effect of above-mentioned experimental example.
Embodiment 1:Granule
Rhizoma Atractylodis Macrocephalae 250g Fructus Aurantii Immaturus (stir-fry) 250g Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) 188g
Fructus Crataegi (Jiao) 250g Semen Raphani (stir-fry) 250g Fructus Citri Sarcodactylis 188g,
Get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 10 times of water gagings, warm macerating 2 hours distills and extracted volatile oil in 2 hours, and collection volatile oil adds the betacyclodextrin of 4 times of amounts and makes clathrate with polishing, and cold drying is standby below 50 ℃; Gained water liquid filters standby; Medicinal residues add 6 times of water gagings again and decocted 30 minutes; Filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adds 10 times of water gagings and decocts 1 hour, filters, medicinal residues add 7 times of water gagings again and decocted 30 minutes, filter, decocting liquids such as the filtrate and the Rhizoma Atractylodis Macrocephalae merge, and are concentrated into 50 ℃ of relative density 1.07-1.09, adding ethanol makes and contains alcohol amount and reach 60%, cold preservation 24 hours filters filtrate recycling ethanol, the clear paste that it is 1.35-1.38 that water liquid continues to be concentrated into 60 ℃ of relative densities, 1 part of qinghuo reagent, 2.5 parts of cane sugar powders, dextrin is made granule in right amount, dry, add volatile oil clathrate compounds such as the above-mentioned Rhizoma Atractylodis Macrocephalae, mixing makes 1000g, promptly get 125 bags, every bag of 8g.
Function with cure mainly: spleen benefiting and stimulating the appetite, dyspepsia and intestinal stasis relieving.It is stagnant to be used for the long-pending type of infantile spleen deficiency folder, and card is seen anorexia, belching and acid regurgitation, and the abdominal part distension, shallow complexion, the asthenia of becoming thin, it is uncomfortable to defecate, thick fur rolling pulse etc.
Usage and consumption: oral, one-year-old in, once obey 2g, one-year-old to three years old, once obey 4g, three years old to seven years old, once obey 6g, seven years old to 12 years old, once obey 8g, 3 times on the one.
Embodiment 2: oral liquid
Rhizoma Atractylodis Macrocephalae 260g Fructus Aurantii Immaturus (stir-fry) 270g Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) 200g
Fructus Crataegi (Jiao) 230g Semen Raphani (stir-fry) 240g Fructus Citri Sarcodactylis 170g,
Get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 11 times of water gagings, warm macerating 3 hours distills and extracted volatile oil in 2.5 hours, and it is standby to collect volatile oil; Gained water liquid filters standby; Medicinal residues add 6 times of water gagings again and decocted 30 minutes; Filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adds 10 times of water gagings and decocts 1 hour, filters, medicinal residues add 8 times of water gagings again and decocted 35 minutes, filter, and decocting liquids such as the filtrate and the Rhizoma Atractylodis Macrocephalae merge, be concentrated into 55 ℃ of relative density 1.07-1.09, add ethanol and make and contain alcohol amount and reach 60%, cold preservation 24 hours, filter, filtrate recycling ethanol, water liquid adds above-mentioned volatile oil with the Tween 80 solubilising, with sucrose 200g, add water to 1000ml, promptly.
Usage and consumption: oral, one-year-old in, once obey 5ml, one-year-old to three years old, once obey 10ml, three years old to seven years old, once obey 15ml, seven years old to 12 years old, once obey 20ml, 3 times on the one.
Embodiment 3:Tablet
Rhizoma Atractylodis Macrocephalae 235g Fructus Aurantii Immaturus (stir-fry) 235g Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) 165g
Fructus Crataegi (Jiao) 265g Semen Raphani (stir-fry) 260g Fructus Citri Sarcodactylis 200g,
Get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 10 times of water gagings, warm macerating 2 hours distills and extracted volatile oil in 2 hours, and collection volatile oil adds the betacyclodextrin of 4 times of amounts and makes clathrate with polishing, and cold drying is standby below 50 ℃; Gained water liquid filters standby; Medicinal residues add 6 times of water gagings again and decocted 30 minutes; Filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adds 10 times of water gagings and decocts 1 hour, filters, and medicinal residues add 7 times of water gagings again and decocted 30 minutes, filter, decocting liquids such as the filtrate and the Rhizoma Atractylodis Macrocephalae merge, and are concentrated into 50 ℃ of relative density 1.07-1.09, add ethanol and make and contain alcohol amount and reach 60%, cold preservation 24 hours filters, the clear paste that it is 1.35-1.38 that filtrate recycling ethanol, water liquid continue to be concentrated into 60 ℃ of relative densities, qinghuo reagent adds appropriate amount of auxiliary materials, granulate, drying adds volatile oil clathrate compounds such as the above-mentioned Rhizoma Atractylodis Macrocephalae, mixing is suppressed 1000, promptly.
Usage and consumption: oral, one-year-old in, once obey 1, one-year-old to three years old, once obey 2, three years old to seven years old, once obey 3, seven years old to 12 years old, once obey 4,3 times on the one.
Embodiment 4:The method of quality control of granule
A. get this product 6g, porphyrize adds water 50ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow goes into till the flask, adds ethyl acetate 2ml again, connects reflux condensing tube, reflux 1 hour is divided and is got the ethyl acetate layer, as need testing solution, other gets Rhizoma Atractylodis Macrocephalae control medicinal material 1g, and 60-90 ℃ adds petroleum ether 15ml, 60 ℃ of water-bath warm macerating 1 hour, filtration, evaporate to dryness, residue add ethyl acetate 1ml dissolving, as Rhizoma Atractylodis Macrocephalae control medicinal material solution; Get Fructus Citri Sarcodactylis control medicinal material 1g again, add dehydrated alcohol 10ml, supersound process 10 minutes filters, and filtrate is concentrated into about 1ml, as Fructus Citri Sarcodactylis control medicinal material solution; Test according to thin layer chromatography (2000 editions appendix VIB of Chinese Pharmacopoeia), draw each 3-6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developing solvent with petroleum ether 60-90 ℃-ethyl acetate at 4: 1, launch, take out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Fructus Citri Sarcodactylis control medicinal material chromatograph on, show identical navy blue speckle; Spray is with 10% ethanol solution of sulfuric acid, and 105 ℃ were dried by the fire 3-5 minute, and put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Rhizoma Atractylodis Macrocephalae control medicinal material chromatograph on, show the fluorescence speckle of same color;
B. get Cortex Magnoliae Officinalis control medicinal material 10g, add water 100ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 3 hours, divide and get the ethyl acetate layer, as Cortex Magnoliae Officinalis control medicinal material solution; Test according to thin layer chromatography (2000 editions appendix VIB of Chinese Pharmacopoeia), draw each 5 μ l of need testing solution and above-mentioned control medicinal material solution, put respectively on same silica gel g thin-layer plate, with petroleum ether (60-90 ℃)--ethyl acetate is developing solvent at 10: 1, launch, take out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;
Assay: get this product fine powder 15g, the accurate title, decide, and puts in the conical flask, precision adds methanol 50ml, close plug, and it is fixed accurately to claim, 100W, 50Hz supersound process 40 minutes is put cold, add methanol and supply weight, filter, discard filtrate just, precision is measured subsequent filtrate 35ml, puts to be concentrated into about 5ml in the water-bath, adds water and stirs molten, transfer pH to 1-2 with 2% hydrochloric acid, use extracted with diethyl ether 3 times, each 20ml, discard ether, water liquid is transferred pH to 9-10 with ammonia, extracts 5 times with the ethyl acetate jolting, each 20ml, merge acetoacetic ester liquid, put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, move to (3g, 100-200 order, column internal diameter 15mm on the alumina column of having handled well, wet method dress post), with methanol 100ml eluting, eluent is put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, moves in the 5ml measuring bottle, adds methanol to scale, shake up, as need testing solution; Other gets the Neosynephrine reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution; According to thin layer chromatography (2000 editions one appendix VI B of Chinese Pharmacopoeia) test, draw need testing solution 4 μ l, reference substance solution 1 μ l and 4 μ l, the cross point is developing solvent with n-butyl alcohol-glacial acetic acid-water (4: 1: 5) upper strata liquid on same silica gel g thin-layer plate respectively, saturated 20 minutes, launch, take out, cold wind dries up, and spray is with 0.5% ethanol solution of ninhydrin, 100 ℃ were dried by the fire about 10 minutes, take out, on cover a same big or small glass plate, seal with adhesive plaster all around; Scan wavelength X according to thin layer chromatography (2000 editions one appendix VI B of Chinese Pharmacopoeia thin layer chromatography scanning)
s=510nm, λ
R=700nm measures test sample trap integrated value and reference substance trap integrated value, calculates, that is, this product contains Fructus Aurantii Immaturus with Neosynephrine (C for every bag
9H
13NO
2), must not be less than 0.80mg.
Claims (13)
1. Chinese medicine compositions with the effect of spleen invigorating removing food stagnancy is characterized in that this pharmaceutical composition made by following raw material medicaments:
Rhizoma Atractylodis Macrocephalae 220-280 weight portion Fructus Aurantii Immaturus 220-280 weight portion Cortex Magnoliae Officinalis 150-220 weight portion
Fructus Crataegi 220-280 weight portion Semen Raphani 220-280 weight portion Fructus Citri Sarcodactylis 150-220 weight portion.
2. pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:
The Rhizoma Atractylodis Macrocephalae 250 weight portion Fructus Aurantii Immaturuss 250 weight portion Cortex Magnoliae Officinalis 188 weight portions
Fructus Crataegi 50 weight portion Semen Raphanis 250 weight portion Fructus Citri Sarcodactylis 188 weight portions.
3. as claim 1,2 described pharmaceutical compositions, it is characterized in that the preferred Fructus Crataegi (parched to brown) of Fructus Crataegi described in this pharmaceutical composition, the preferred Fructus Aurantii Immaturus (parched) of Fructus Aurantii Immaturus, the preferred Sichuan Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) of Cortex Magnoliae Officinalis, the preferred Semen Raphani (parched) of Semen Raphani.
4. preparation of drug combination method as claimed in claim 3, it is characterized in that this method is: get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 8-12 times of water gaging, warm macerating 1-3 hour, distill and extracted volatile oil in 1-3 hour, collecting volatile oil adds the betacyclodextrin that 3-5 doubly measures and makes clathrate with polishing, cold drying below 60-30 ℃ is standby, and gained water liquid filters standby, and medicinal residues add 5-7 times of water gaging again and decocted 20-40 minute, filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adding 8-12 times of water gaging decocted 0.5-2 hour, filter, medicinal residues add 6-8 times of water gaging again and decocted 20-40 minute, filter, decocting liquid such as the filtrate and the Rhizoma Atractylodis Macrocephalae merges, be concentrated into 40-60 ℃ of relative density 1.0-1.15, add ethanol and make and contain alcohol amount and reach 50-65%, cold preservation 20-30 hour, filter, the clear paste that it is 1.30-1.45 that filtrate recycling ethanol, water liquid continue to be concentrated into 50-70 ℃ of relative density, volatile oil clathrate compounds such as the qinghuo reagent and the above-mentioned Rhizoma Atractylodis Macrocephalae, through conventional operation directly or add pharmaceutically acceptable excipient and make clinical acceptable forms, as tablet, oral liquid, capsule, granule.
5. preparation of drug combination method as claimed in claim 4, the preparation method that it is characterized in that granule is: get the Rhizoma Atractylodis Macrocephalae, Fructus Aurantii Immaturus, Fructus Citri Sarcodactylis, Cortex Magnoliae Officinalis four Chinese medicine, add 10 times of water gagings, warm macerating 2 hours, distill and extracted volatile oil in 2 hours, collection volatile oil adds the betacyclodextrin of 4 times of amounts and makes clathrate with polishing, and cold drying is standby below 50 ℃; Gained water liquid filters standby; Medicinal residues add 6 times of water gagings again and decocted 30 minutes; Filter collecting decoction; Other gets Fructus Crataegi, Semen Raphani two flavor medicines, adding 10 times of water gagings decocted 1 hour, filter, medicinal residues add 7 times of water gagings again and decocted 30 minutes, filter, decocting liquid such as the filtrate and the Rhizoma Atractylodis Macrocephalae merges, be concentrated into 50 ℃ of relative density 1.07-1.09, add ethanol and make and contain alcohol amount and reach 60%, cold preservation 24 hours, filter, it is 1.35～1.38 clear paste that filtrate recycling ethanol, water liquid continue to be concentrated into 60 ℃ of relative densities, 1 part of qinghuo reagent, 2.5 parts of cane sugar powders, dextrin is made granule in right amount, and drying adds volatile oil clathrate compounds such as the above-mentioned Rhizoma Atractylodis Macrocephalae, mixing makes granule.
6. as the sharp method of quality control that requires 3 described drug combination preparations, it is characterized in that discrimination method in this method comprises one or more in the following discriminating:
A. get this composite preparation 6g, add water 50ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, adds water from determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 1-2 hour, divide and get the ethyl acetate layer, as need testing solution, other gets Rhizoma Atractylodis Macrocephalae control medicinal material 1g, 60-90 ℃ adds petroleum ether 15ml, 50-70 ℃ of water-bath warm macerating 1 hour, filtration, evaporate to dryness, residue add ethyl acetate 1ml dissolving, as Rhizoma Atractylodis Macrocephalae control medicinal material solution; Get Fructus Citri Sarcodactylis control medicinal material 1g again, add dehydrated alcohol 10ml, supersound process 8-15 minute, filter, filtrate is concentrated into about 1ml, as Fructus Citri Sarcodactylis control medicinal material solution; According to the thin layer chromatography test, draw each 3-6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developing solvent with 60-90 ℃ of petroleum ether-ethyl acetate 3-5: 1-2, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Fructus Citri Sarcodactylis control medicinal material chromatograph on, show identical navy blue speckle; Spray is with 10% ethanol solution of sulfuric acid, and 100-115 ℃ was dried by the fire 2-7 minute, and put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Rhizoma Atractylodis Macrocephalae control medicinal material chromatograph on, show the fluorescence speckle of same color;
B. get Cortex Magnoliae Officinalis control medicinal material 10g, add water 100ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 2-4 hour, divide and get the ethyl acetate layer, as Cortex Magnoliae Officinalis control medicinal material solution; According to the thin layer chromatography test, draw each 5 μ l of need testing solution and above-mentioned control medicinal material solution, put respectively on same silica gel g thin-layer plate, be developing solvent with 60-90 ℃ of petroleum ether-ethyl acetate 8-12: 1-2, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
7. as the sharp method of quality control that requires 6 described drug combination preparations, the discrimination method that it is characterized in that granule comprises one or more in the following discriminating:
A. get this product 6g, porphyrize adds water 50ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow goes into till the flask, adds ethyl acetate 2ml again, connects reflux condensing tube, reflux 1 hour is divided and is got the ethyl acetate layer, as need testing solution, other gets Rhizoma Atractylodis Macrocephalae control medicinal material 1g, and 60-90 ℃ adds petroleum ether 15ml, 60 ℃ of water-bath warm macerating 1 hour, filtration, evaporate to dryness, residue add ethyl acetate 1ml dissolving, as Rhizoma Atractylodis Macrocephalae control medicinal material solution; Get Fructus Citri Sarcodactylis control medicinal material 1g again, add dehydrated alcohol 10ml, supersound process 10 minutes filters, and filtrate is concentrated into about 1ml, as Fructus Citri Sarcodactylis control medicinal material solution; According to the thin layer chromatography test, draw each 3-6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developing solvent at 4: 1 with 60-90 ℃ of petroleum ether-ethyl acetate, launch, take out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Fructus Citri Sarcodactylis control medicinal material chromatograph on, show identical navy blue speckle; Spray is with 10% ethanol solution of sulfuric acid, and 105 ℃ were dried by the fire 3-5 minute, and put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Rhizoma Atractylodis Macrocephalae control medicinal material chromatograph on, show the fluorescence speckle of same color;
B. get Cortex Magnoliae Officinalis control medicinal material 10g, add water 100ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 3 hours, divide and get the ethyl acetate layer, as Cortex Magnoliae Officinalis control medicinal material solution; Test according to thin layer chromatography, draw each 5 μ l of need testing solution and above-mentioned control medicinal material solution, put respectively on same silica gel g thin-layer plate, be developing solvent with 60-90 ℃ of petroleum ether-ethyl acetate at 10: 1, launch, take out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
8. as the sharp method of quality control that requires 3 described drug combination preparations, it is characterized in that the content assaying method in this method is: get this composite preparation fine powder 15g, the accurate title, decide, put in the conical flask, precision adds methanol 50ml, close plug, it is fixed accurately to claim, supersound process 30-50 minute, puts cold, add methanol and supply weight, filter, discard filtrate just, precision is measured subsequent filtrate 35ml, puts to be concentrated into about 5ml in the water-bath, adds water 10ml and stirs molten, transfer pH to 1-2 with 2% hydrochloric acid, with extracted with diethyl ether 3 times, each 20ml discards ether solution, water liquid is transferred pH to 9-10 with ammonia, extract 5 times with the ethyl acetate jolting, each 20ml merges ethyl acetate liquid, put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, moves on the alumina column of having handled well, with methanol 100ml eluting, eluent is put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, moves in the 5ml measuring bottle, adds methanol to scale, shake up, as need testing solution; Other gets the Neosynephrine reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution; According to the thin layer chromatography test, draw need testing solution 4 μ l, reference substance solution 1 μ l and 4 μ l, respectively the cross point is on same silica gel g thin-layer plate, and with 3-5: 1-2: 4-6 n-butyl alcohol-glacial acetic acid-layer liquid waterborne is developing solvent, saturated 15-25 minute, launch, take out, cold wind dries up, and spray is with 0.5% ethanol solution of ninhydrin, 90-110 ℃ was dried by the fire about 8-12 minute, take out, on cover a same big or small glass plate, seal with adhesive plaster all around; Scan wavelength X according to thin layer chromatography
s=510nm, λ
R=700nm measures test sample trap integrated value and reference substance trap integrated value, calculates, that is, this composite preparation per unit amount contains Fructus Aurantii Immaturus with Neosynephrine, must not be less than 0.80mg.
9. as the sharp method of quality control that requires 8 described pharmaceutical preparatioies, it is characterized in that the content assaying method of granule is:
Get this product fine powder 15g, the accurate title, decide, and puts in the conical flask, precision adds methanol 50ml, close plug, and it is fixed accurately to claim, 100W, 50Hz supersound process 40 minutes is put cold, add methanol and supply weight, filter, discard filtrate just, precision is measured subsequent filtrate 35ml, puts to be concentrated into about 5ml in the water-bath, adds water 10ml and stirs molten, transfer pH to 1-2 with 2% hydrochloric acid, use extracted with diethyl ether 3 times, each 20ml, discard ether solution, water liquid is transferred pH to 9-10 with ammonia, extracts 5 times with the ethyl acetate jolting, each 20ml merges ethyl acetate liquid, puts evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, moves on the alumina column of having handled well, with methanol 100ml eluting, eluent is put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, moves in the 5ml measuring bottle, adds methanol to scale, shake up, as need testing solution; Other gets the Neosynephrine reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution; According to the thin layer chromatography test, draw need testing solution 4 μ l, reference substance solution 1 μ l and 4 μ l, the cross point is developing solvent with 4: 1: 5 n-butyl alcohol-glacial acetic acid-layer liquid waterborne on same silica gel g thin-layer plate respectively, saturated 20 minutes, launch, take out, cold wind dries up, and spray is with 0.5% ethanol solution of ninhydrin, 100 ℃ were dried by the fire about 10 minutes, take out, on cover a same big or small glass plate, seal with adhesive plaster all around; Scan wavelength X according to thin layer chromatography
s=510nm, λ
R=700nm measures test sample trap integrated value and reference substance trap integrated value, calculates, that is, this product contains Fructus Aurantii Immaturus with Neosynephrine for every bag, must not be less than 0.80mg.
10. method of quality control as claimed in claim 9 is characterized in that alumina column described in the assay is 3g, 100-200 order, column internal diameter 15mm, wet method dress post.
11., it is characterized in that this method may further comprise the steps as the sharp method of quality control that requires 3 described drug combination preparations:
Differentiate: a. gets this composite preparation 6g, add water 50ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water makes and is full of the scale part from determinator upper end, and overflow goes into till the flask, adds ethyl acetate 2ml again, connects reflux condensing tube, reflux 1-2 hour, divide and get the ethyl acetate layer, as need testing solution, other gets Rhizoma Atractylodis Macrocephalae control medicinal material 1g, 60-90 ℃ adds petroleum ether 15ml, 50-70 ℃ of water-bath warm macerating 1 hour, filtration, evaporate to dryness, residue add ethyl acetate 1ml dissolving, as Rhizoma Atractylodis Macrocephalae control medicinal material solution; Get Fructus Citri Sarcodactylis control medicinal material 1g again, add dehydrated alcohol 10ml, supersound process 8-15 minute, filter, filtrate is concentrated into about 1ml, as Fructus Citri Sarcodactylis control medicinal material solution; According to the thin layer chromatography test, draw each 3-6 μ l of above-mentioned solution, put respectively on same silica gel g thin-layer plate, be developing solvent with 60-90 ℃ of petroleum ether-ethyl acetate 3-5: 1-2, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Fructus Citri Sarcodactylis control medicinal material chromatograph on, show identical navy blue speckle; Spray is with 10% ethanol solution of sulfuric acid, and 100-115 ℃ was dried by the fire 2-7 minute, and put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of Rhizoma Atractylodis Macrocephalae control medicinal material chromatograph on, show the fluorescence speckle of same color;
B. get Cortex Magnoliae Officinalis control medicinal material 10g, add water 100ml, put at the bottom of the garden in the flask, on connect volatile oil determination apparatus, add water from the determinator upper end and make and be full of the scale part, and overflow is gone into till the flask, add ethyl acetate 2ml again, connect reflux condensing tube, reflux 2-4 hour, divide and get the ethyl acetate layer, as Cortex Magnoliae Officinalis control medicinal material solution; According to the thin layer chromatography test, draw each 5 μ l of need testing solution and above-mentioned control medicinal material solution, put respectively on same silica gel g thin-layer plate, be developing solvent with 60-90 ℃ of petroleum ether-ethyl acetate 8-12: 1-2, launch, take out, dry, put under the ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;
Assay: get this composite preparation fine powder 15g, the accurate title, decide, and puts in the conical flask, precision adds methanol 50ml, close plug, and it is fixed accurately to claim, supersound process 30-50 minute, put coldly, add methanol and supply weight, filter, discard filtrate just, precision is measured subsequent filtrate 35ml, put and be concentrated into about 5ml in the water-bath, add water 10ml and stir moltenly, transfer pH to 1-2 with 2% hydrochloric acid, with extracted with diethyl ether 3 times, each 20ml discards ether solution, water liquid is transferred pH to 9-10 with ammonia, extracts 5 times with the ethyl acetate jolting, each 20ml, merge ethyl acetate liquid, put evaporate to dryness in the water-bath, residue adds methanol makes dissolving in right amount, move on the alumina column of having handled well, with methanol 100ml eluting, eluent is put evaporate to dryness in the water-bath, and residue adds methanol makes dissolving in right amount, move in the 5ml measuring bottle, add methanol to scale, shake up, as need testing solution; Other gets the Neosynephrine reference substance, adds methanol and makes the solution that every 1ml contains 0.5mg, in contrast product solution; According to the thin layer chromatography test, draw need testing solution 4 μ l, reference substance solution 1 μ l and 4 μ l, respectively the cross point is on same silica gel g thin-layer plate, and with 3-5: 1-2: 4-6 n-butyl alcohol-glacial acetic acid-layer liquid waterborne is developing solvent, saturated 15-25 minute, launch, take out, cold wind dries up, and spray is with 0.5% ethanol solution of ninhydrin, 90-110 ℃ was dried by the fire about 8-12 minute, take out, on cover a same big or small glass plate, seal with adhesive plaster all around; Scan wavelength X according to thin layer chromatography
s=510nm, λ
R=700nm measures test sample trap integrated value and reference substance trap integrated value, calculates, that is, this composite preparation per unit amount contains Fructus Aurantii Immaturus with Neosynephrine, must not be less than 0.80mg.
12. Chinese medicine composition as claimed in claim 3 has application in the spleen invigorating removing food stagnancy drugs with function in preparation.
13. application as claimed in claim 12 is characterized in that the application in preparation treatment infantile malnutrition medicine.
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| CN102579784A (en) * | 2011-12-21 | 2012-07-18 | 江西济民可信药业有限公司 | Preparation method of tonifying spleen granule for infants |
| CN103100043A (en) * | 2012-12-10 | 2013-05-15 | 宋爱民 | Traditional Chinese medicine preparation for treating children indigestion symptom |
| CN103134883A (en) * | 2011-11-28 | 2013-06-05 | 邯郸摩罗丹药业股份有限公司 | Medicine composition and detecting method of manufacturing agent of medicine composition |
| CN104483437A (en) * | 2014-12-10 | 2015-04-01 | 常熟雷允上制药有限公司 | Quality detection method of anti-tumour traditional Chinese medicine composition |
| CN105942065A (en) * | 2016-05-26 | 2016-09-21 | 孙明香 | Fructus crataegi beverage for promoting ingestion and preparation method of fructus crataegi beverage |
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2003
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103134883A (en) * | 2011-11-28 | 2013-06-05 | 邯郸摩罗丹药业股份有限公司 | Medicine composition and detecting method of manufacturing agent of medicine composition |
| CN103134883B (en) * | 2011-11-28 | 2015-05-13 | 邯郸制药股份有限公司 | Medicine composition and detecting method of formula of medicine composition |
| CN102579784A (en) * | 2011-12-21 | 2012-07-18 | 江西济民可信药业有限公司 | Preparation method of tonifying spleen granule for infants |
| CN103100043A (en) * | 2012-12-10 | 2013-05-15 | 宋爱民 | Traditional Chinese medicine preparation for treating children indigestion symptom |
| CN103100043B (en) * | 2012-12-10 | 2014-07-16 | 宋爱民 | Traditional Chinese medicine preparation for treating children indigestion symptom |
| CN104483437A (en) * | 2014-12-10 | 2015-04-01 | 常熟雷允上制药有限公司 | Quality detection method of anti-tumour traditional Chinese medicine composition |
| CN104483437B (en) * | 2014-12-10 | 2016-06-15 | 常熟雷允上制药有限公司 | A kind of quality determining method of antitumor Chinese |
| CN105942065A (en) * | 2016-05-26 | 2016-09-21 | 孙明香 | Fructus crataegi beverage for promoting ingestion and preparation method of fructus crataegi beverage |
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| CN1244350C (en) | 2006-03-08 |
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