CN1384769A - Mixing apparatus and method of mixing - Google Patents

Mixing apparatus and method of mixing Download PDF

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Publication number
CN1384769A
CN1384769A CN00808712A CN00808712A CN1384769A CN 1384769 A CN1384769 A CN 1384769A CN 00808712 A CN00808712 A CN 00808712A CN 00808712 A CN00808712 A CN 00808712A CN 1384769 A CN1384769 A CN 1384769A
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CN
China
Prior art keywords
stirring vane
instrument
chamber
sample
sample container
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Pending
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CN00808712A
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Chinese (zh)
Inventor
戴维·安德鲁斯
约翰·A·奥德尔
安东尼·史蒂文森
约翰·柯蒂斯
艾德里安·R·格雷
约翰·P·维西
菲利克斯·费尔南多
马克·克雷斯韦尔
戴维·A·珀西瓦尔
约翰·W·阿特里奇
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Provalis Diagnostics Ltd
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Provalis Diagnostics Ltd
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Publication date
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Publication of CN1384769A publication Critical patent/CN1384769A/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/726—Devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/441—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/50—Movable or transportable mixing devices or plants
    • B01F33/501—Movable mixing devices, i.e. readily shifted or displaced from one place to another, e.g. portable during use
    • B01F33/5011—Movable mixing devices, i.e. readily shifted or displaced from one place to another, e.g. portable during use portable during use, e.g. hand-held
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/049—Valves integrated in closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0474—Details of actuating means for conveyors or pipettes
    • G01N2035/0491—Position sensing, encoding; closed-loop control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/022—Casings
    • G01N2201/0221—Portable; cableless; compact; hand-held

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Optical Measuring Cells (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Apparatus, instrument, device and a method of mixing for conducting an assay. Apparatus comprising a first and second inlet and an inlet port accommodating a filter and/or binder retaining means, the inlet port moveable relative to first and second inlets such that the port (9) can be brought into liquid communication with each inlet, and a sample chamber (3, 5) comprising a paddle (100) wich undergeos a reciprocating motion.

Description

Mixing arrangement and mixed method
The present invention relates to a kind of improved mixing arrangement and a kind of mixed method.
Especially, the present invention relates to a kind of method of improved, the device, instrument and equipment and the chemical examination that are used to chemically examine.
In a particularly preferred embodiment, the present invention relates to a kind of device that is applicable to assay, for example in blood sample, freezing (glaciated) protein is carried out assay.
But, it will be appreciated by those skilled in the art that principle of the present invention can be used in the mixed problem of solution in multiple different device, instrument or equipment.
The applicant has developed a kind of device that is used to chemically examine, instrument and equipment, described in PCT/GB98/03586.This device comprises first import, second import and inlet hole, this inlet hole can move with respect to each described first and second import, like this, this hole can make liquid be communicated with required each import successively, and this inlet hole is equipped with filter for installation and/or bonding agent holding device.
When chemically examining so that when for example determining in sample, to be with or without one or more analytes, sample is separated into first and second portion, and this second portion is to obtain by the composition of elution from the bonding agent holding device " maintenance " on the bonding agent holding device.
The applicant thinks, will produce uneven sample (owing to forming the elution gradient in second import) in the elution step that makes eluent be full of second import under the gravity effect, and reading was incorrect when this caused " reading " sample in measuring instrument.For example, comprise can be by the microprocessor of keyboard operation, one or more optical transmitting set and one or more photodetectors, display with driver, an analog-digital converter and be used for device that this instrument is linked to each other with power supply for this instrument.
An object of the present invention is to provide a kind of simple method, be used to make gravity to supply with part evenly, a kind of improved device, instrument and/or the equipment that can realize this method especially is provided.
According to a first aspect of the invention, provide a kind of in chamber the method for composite sample, this method comprises to be made stirring vane be positioned at sample and described stirring vane is moved back and forth.
Another independent purpose of the present invention provide a kind of can composite sample so that the device of chemically examining, instrument or equipment, wherein, by the spectrophotometric counter device and/or be used to realize that the parts of this purpose come the check and analysis thing.
According to a further aspect in the invention, provide a kind of stirring vane, having comprised: liquid moving surface and be used for described stirring vane is bearing in device in the chamber or on the chamber, thus this stirring vane can be moved back and forth in this chamber.
Preferably, be used for the device that stirring vane is bearing in the chamber or on the chamber is comprised a pair of arm that stretches out from this liquid moving surface.
Preferably, this stirring vane becomes T shape.
Be more preferably, this liquid moving surface has the opening that is formed at wherein, and light beam can pass through this opening.
In one embodiment, stirring vane comprises a magnetic material, and by a calutron for example solenoid it is moved back and forth.
Certainly, also can realize moving back and forth with other mechanism.For example, this stirring vane comprises a piezoelectric, and utilizes local current and it is moved back and forth.
According to another aspect of the invention, provide a kind of sample container that is used to install stirring vane, described stirring vane is installed in the described sample container or on the sample container, like this, this stirring vane can move back and forth in container.
Preferably, this sample container comprises a pedestal, and this pedestal has the sidewall that extends from it, and to determine a chamber, described sidewall comprises the device that supports this stirring vane, for example a pair of slit.
Preferably, this sample container is the device that comprises an optical cell.
Be more preferably, this sample container is the part of carousel or box.
According to a further aspect in the invention, provide a kind of instrument that is used to install sample container, this sample container comprises a stirring vane, and described instrument comprises the device that described stirring vane is moved back and forth in container.
Preferably, the described device that is used to described stirring vane is moved back and forth is a calutron, for example solenoid.
According to a further aspect in the invention, provide a kind of equipment that reads instrument that comprises, it comprises the device that is used for driving in comprising the device of optical cell in reciprocating mode stirring vane.
Can adopt device of the present invention and instrument as described in International Application PCT/GB98/03586.
At device described in the PCT/GB98/03586 and instrument a plurality of other problems total with the equipment that is used to chemically examine are arranged.Therefore, one with the device-independent independent problem of the general type described in the PCT/GB98/03586, promptly, giving instrument with sample so that the problem when reading data, is to avoid read error or making this read error hour make with respect to one or more optical transmitting sets of forming reading device and one or more photodetector and the accurately problem of location of this sample at least.
Therefore, an independent purpose of the present invention provides a kind of device that can accurately read.
According to this independent aspects of the present invention, a kind of equipment is provided, this equipment comprises the device that is used to read the instrument of one or more samples and is used for one or more samples are given this instrument, wherein, one or more samples are positioned to read the position be to utilize two stage identification (recognition) to obtain.
This two stage identification preferably utilizes at least two independently micro switch.
First switch sends the information of the scope of living in of this device to this instrument, and second switch is confirmed accurately alignment.
Excite by " element " on this device in first micro switch on the instrument, this constitutes the phase I of detecting.Preferably, this element on device is a protruding part, and this protruding part forces down the plate that micro switch is installed by the rocker arm assembly.The rocker arm action has overcome any error of the horizontal level of this switch on circuit board.
Second switch on the instrument plays the effect of " fine setting ", and excites when instrument arrives accurate (with common opposite) position of this instrument.
In one embodiment, two of switch parts are groove and the elastic component on instrument or the arms on the outermost sidewall of device (especially carousel or box type device).When carousel or box type device move when in place, elastic component or arm move to the position that is not subjected to bias voltage from the position that is subjected to bias voltage, thereby make this switch inertia.
This two stage identification makes that assembling is easier, and has increased the reliability of work.It can also improve the convenience of use.
For the carousel of type described in the PCT/GB98/03586, a plurality of such switches are preferably arranged, be more preferably four such switches of 90 ° of distributions at interval.
With the device-independent independent problem of the general type described in the PCT/GB98/03586 is how two different pieces to be carried out carry out good reading when quantitative.
For example, in treating diabetes, wish to determine the percentage of freezing blood hemoglobin (Hb).This means to obtain two results of laboratory test, and they are compared.For example, between freezing and not freezing hemoglobin, compare.
Traditional analysis is measured when crest frequency.When measurement contains the freezing protein of hemochrome, the about 405nm of this crest frequency.The applicant thinks that measuring to have tangible advantage when offset peak, when measuring freezing hemoglobin between 415-460nm, especially when about 440nm.This frequency range is corresponding to the shoulder part of the absorbing wavelength curve of hemoglobin.440nm is an optimal wavelength.At this moment it extends linear response, thereby covers wideer and more useful HC scope.
According to this independent aspects of the present invention, the method of the % of a kind of definite blood glycosylation (glycation) is provided, and this method comprises: blood sample is divided into includes one or more not firsts and the second portion that includes one or more freezing protein of freezing protein; (be more preferably) between 405nm and 460nm by the spectrophotometric counter device analyte is carried out detected/quantified at about 440nm.
The measurement of " offset peak " will be avoided the calibration steps of the complexity in result treatment and the process that experimentizes.For the performance of instrument, when the contrast experiment is when carrying out between instrument, measure and absorption base concentration between linear response is arranged will be very important.It is also important that the scope of this linear response is enough wide, like this, measurement for example freezing and not glycosylation part can both be carried out in the linear segment of response curve.This is that the slope of linear response will change owing to a variety of causes because for different instruments.And in an instrument, the slope of this linear response also can be used as the function of temperature or other environmental factor and changes.But, the estimated performance of glycosylated % is like this, and in given instrument, response slope is cancelled out each other.Therefore, slope variation can not influence in a device or the result who is measured between stream oriented device, as long as do not have obvious variation in the interval of chemical examination.And all the other variations between device can compensate by the skew of the scale in the process of initial setting during fabrication.
Adopt the wavelength of narrow wave band will form linear response, but when the absorption maximum of the close hemoglobin of this wave band, the scope of response reduce.This is because in this some place, the sensitivity maximum of system.Selection departs from the sensitivity that the peaked suitable band filter of absorption will reduce system, and prolongs the working range of response, and can eliminate above-mentioned slope factor.Then, the sensitivity to this reduction compensates by obtain very high signal noise ratio in the detector electronics element.
One is to guarantee the accuracy read and make critical value (CV ' s) minimum with the device-independent independent problem of the general type described in the PCT/GB98/03586.
The applicant thinks that the principal element that influences critical value is to guarantee to concentrate whole samples so that measure.Therefore, when measuring less volume, about 8% in this total measurement (volume) may drip middle loss at a dropping liquid.
According to this independent aspects of the present invention, a kind of device is provided, this device comprises one or more capillary devices that are used for stranded blob, leaves the second portion of first and access to plant so that guarantee it.
In one embodiment, this device comprises a web or a like between first and second parts.
Especially, this device is such type, and promptly inlet hole can move with respect to each first and second import, and described inlet hole is an infundibulate, and filter for installation or bonding agent holding device are housed, and the outlet of described funnel is crossed in the position of described web.
One is to guarantee problem that this device firmly is held in place with the device-independent question of independence of the general type described in the PCT/GB98/03586.
The applicant has solved this problem by careful design apparatus and instrument.
Like this, in one embodiment, carousel comprises a taper circumferential hoop, and this instrument comprises spring clip, and this spring clip pushes away carousel to prevent swing downwards.
According to this independent aspects of the present invention, a kind of equipment is provided, this equipment comprises a kind of instrument and a kind of device that is used for these one or more samples are given instrument that is used to read one or more samples, and wherein, this device firmly is held in place in this instrument by spring clip.
Introduce main invention of the present invention and each independent aspects below with reference to described general type equipment of PCT/GB98/03586 and the method for chemically examining freezing and not freezing hemoglobin part.
Fig. 1 is the perspective view of device according to an aspect of the present invention;
Fig. 2 is the partial sectional view of the device of Fig. 1;
Fig. 3 is the perspective view of the base part of the device among Fig. 2, represents stirring vane of the present invention; And
Fig. 4 has adopted the perspective view of the instrument of device as shown in Figures 1 to 3.
With reference to Fig. 1 and 2, carousel (carousel) 31 comprises base part 2 (seeing Fig. 3 in detail), a top section 6 and funnel part 32 that a transparent plastic is made.This funnel part 32 is made by the hydrophobicity plastics, and relatively large hole is arranged, so that can simplify the emptying of reagent wherein.It has an outlet 34, and when this device rotated in instrument, this outlet 34 inserted the liquid in optical cell 3 and 5.Outlet 34 comprises a sinter (frit), and this sinter (not shown) plays for example effect of aminophenyl borate (boronate) agarose affinity substrate of particulate that keeps.The funnel 32 that plays the inlet hole effect has an annular lip 36 with notch part 38.Flange 36 overlaps with the hole 40,42 and 44 parts that are formed in this device top 6, like this, aligns with the notch part 38 of annular lip up to this hole, and the Guan Caineng that is arranged vertically in this device passes corresponding hole.What protrude from the bottom side of funnel is bar 48, and this bar 48 has cloudy counterpart, and device 31 links to each other with instrument 24 by this moon counterpart, and this instrument 24 has one to be used for the positive counterpart 50 that matches with this moon counterpart.Sun counterpart 50 makes funnel be maintained fixed the position with respect to instrument 24, and like this, the base part 2 and the top section 6 of device 31 form carousel together, and around this funnel rotation, the annular lip 36 of funnel plays the effect of guiding device.
The base part 6 of device is made by transparent plastic, and totally circularizes and be divided into a plurality of separate spaces.As shown in Figure 3, two optical cell 3 and 5, one the 3rd chambers 4 and three further chambers 40 ', 42 ' and 44 ' that the reagent pipe respectively is housed are arranged, the 3rd chamber 4 is used to accept the rubbish that rinsing step produces, and the 3rd chamber is arranged between optical cell 3 and 5.The hole 40,42 and the 44 following chambers 40 ', 42 ' and 44 ' that are arranged in the top section 6 of device 31 are arranged to like this, and promptly when this carousel in use was in correct position, the reagent pipe can give the user.Optical cell has crooked outer wall 52 and crooked inwall 54, and the light that the optical characteristics of this curved outer wall 52 and curved inner wall 54 helps the LED by device 24 to be sent and passes the sample in the chamber focuses on the photodiode of opposite side.
Each optical cell 3,5 can with outlet 34 fluid connections in funnel inlet hole 9.Perhaps, this optical cell can be a spill.Guiding piece 58 is protruding from the outermost sidewall 56 of base part 2, and this guiding piece 58 is located in the circumferential channel member 60 that forms on the outermost sidewall 62 of annular notch 64 of instrument 24.When device 31 linked to each other with instrument 24, the connection conduit 66 that the channel member 60 from the outermost sidewall is stretched to the end face 68 of instrument 24 made this guiding piece 58 can insert in the channel member 60.
Protruding part on the knurled edge 72 of top section 6 or lug 70 are represented this device residing position in instrument as indicating device, also help to rotate this device simultaneously.
Base part 2 links to each other with top section, and funnel part is positioned at conduit 76, and this conduit 76 is formed by the step on the top surface 78 of top section 6.
Instrument shown in Figure 4 is designed to adopt as the aforesaid basic device of this paper.Dynamic operation of this instrument and supervisory circuit, like this, this instrument can link to each other with for example outside dc power supply or car battery.In addition, this device has communication system, and for example RS232 instructs and the download data so that send and receive.
Importantly, the device that is used for installing this device is the annular notch 64 at this instrument, and this annular notch 64 is determined by base plate, outermost sidewall 62 and inside side walls 80.
The base plate of annular notch is included in the inclined-plane 82 on the part of this base plate.In the outermost sidewall 62 of this annular notch, channel member 60 is arranged, and a connection conduit 66 stretches to end face from this channel member 60.
In use, the guiding piece 58 of this basic device by making device be connected conduit 66 and align and insert in the annular notch 64, like this, this device is by its cloudy counterpart 48 and link to each other with positive counterpart 50.Like this, guiding piece 58 can enter in the channel member 60, so this device can rotate.When rotated, first pipe is drawn ramp 82 and is drawn from hole 44, because the notch part 38 of annular ring 36 aligns with this hole.In this position, outlet 34 and first optical cell 3 fluid connections, and chemically examine the first step of work.By making this device turn over 90 ° again, cleaning solution exposes so that use by hole 42, and then makes this device turn over 90 °, exposes elutriant from hole 40.Each step in assay process all has suitable reagent like this.
Simply introduced after basic device and the instrument, be elaborated from the improvement aspect below.
During experiment, the described device of PCT/GB98/03586 shows that critical value is about 6-7%.And find that this depends primarily on formed elution gradient when freezing part elution breaks away from solid phase.In fact find that when the elution buffer was penetrated in the optical cell 5, the concentration of the freezing part of institute's elution reduced.Experiment shows that the drop of first concentration of coming out concentrates on the corner of optical cell 5 from funnel 32, and does not have fully to mix with rarer subsequently drop.Therefore, the lower and back measured value that is write down of " departing from " mixing in the measured value accuracy of this solution being carried out carried out before the mechanical mixture.
In order to overcome this problem, need to introduce blend step.
But, commonsense method is proved to be inappropriate.For example this device can not vibrate need not fearing to damage under the situation of instrument, and uses rotation flea or vibrating ball bearing may damage optical cell.
The application utilizes stirring vane 100 to solve problem.It has adopted a plurality of schemes:
The maintenance of agitating device is considered to the subject matter that needs solve.Mixing component is installed on the sidewall of optical cell and is considered to a kind of feasible method that can overcome this problem.It is contemplated that two kinds of optional modes: in one embodiment, stirring vane is clipped on the sidewall of optical cell, and utilizes electromagnet to make the direction vibration of this stirring vane along optical axial.The passage of the light that sends from LED is provided in the hole at stirring vane center.
In another embodiment, stirring vane is clipped on the side of optical cell 5, and to leave this optical channel with the rectangular direction of optical axial and to vibrate.
In case the frequency of the oscillator by regulating DM is set up resonant frequency, two embodiment can both provide sufficient mixing.Although also attractive, this method also has several problems.
Because stirring vane must keep certain rigidity, therefore need sizable energy to produce vibration.This is very important to adopting battery operated instrument.
And, the resonant frequency difference of each parts, and this resonant frequency changes with the liquid level in the chamber.Therefore need the device of some scan frequency,, thereby guarantee fully to mix so that find resonant frequency.Because two parts all are 3 dimension shapes, therefore, the required cost of shaping increases.
A kind of optional method of a flat stirring vane that utilizes has overcome the problem relevant with above-mentioned method for oscillating.
Therefore, in a preferred embodiment, as shown in Figure 3, metal stirring vane 100 remains in the groove 101, and this groove forms by the sidewall 102,104 that forms optical cell 5.Stirring vane can move back and forth with minimal friction forces, and can utilize electromagnet and force stirring vane to pass solution and swing along the optical axial direction, this electromagnet be located at photodiode on flat-die spare (below will the introduce) periphery below.Hole 106 gets at the luminous energy that sends from LED to reach detector.
Because it is very little to move the required power of this stirring vane, so the required energy of DM is quite little.Experiment shows, only needs this stirring vane swing just can produce visible homogeneous solution by the colored water starting soln of layering less than 10 times.
Verified, following and lucky on the center of stirring vane by making a web (not shown) be positioned at top module 6, can effectively keep this stirring vane.
Another improved form relates to 2 micro switch of employing in (phased) method stage by stage.Can accurately, clearly detect the device 31 in instrument 24 like this.During rotation, a switch (not shown) at each place in four positions excites by the parts on the plastic wall periphery 58 (at this moment it also is a guiding piece).This has forced down the plate that the micro switch (not shown) is installed by the rocker arm assembly of locating at four operating positions (Fig. 4) (110,112,114 and 116).The error that excites the horizontal level that has overcome the switch on the circuit board (not shown) of rocker arm, this has constituted the phase I of detecting.
The second stage that detects is undertaken by micro switch, and this micro switch excites by the operation of unshowned arm of ratchet and the corresponding recesses 120,122 on the outermost sidewall 56 of carousel (have only in four two visual).The flange 131 that stretches out from arm of ratchet contacts with the switch on instrument.This arm of ratchet is biased into like this, when carousel is in four operating positions one, arm of ratchet moves in the groove in the carousel, thereby make the switch inertia, but when it is not in these positions, it is against the outermost sidewall 56 of carousel, thereby excites this switch.These grooves preferably form like this, promptly only allow one-directional rotation.When instrument and device were in accurately the position, these switches are inertias.This two stage method makes that assembling is easier, and has increased the stability of work and improved the convenience of using.
At last, another improved form relates to the structure that is used to overcome " swing " problem.Any motion between this carousel and instrument (even little motion) can change the passage that reads light in the process.By improving carousel and instrument, can overcome this and read problem so that a locking device to be provided.
In one embodiment, carousel comprises (Fig. 1) circumferential hoop 124, and this circumferential hoop comprises an inclined surface 126 and a plane surface 128.
The instrument 24 that this carousel is installed comprises: a housing 130; One printed substrate 132, platen 134 are installed on this printed substrate 132; And a pressing plate 136, this pressing plate 136 comprises four spring clips 138,140,142,144.In the time of in carousel is inserted into this instrument, spring clip is pressed on the inclined surface 126, and their tooth lock leans against on the plane surface 128.

Claims (35)

1. method at the interior composite sample of chamber (3,5), this method comprise to be made stirring vane (100) be positioned at sample and described stirring vane is moved back and forth.
2. method according to claim 1, wherein: this stirring vane is a magnetic material, and comprise the liquid moving surface and be used for described stirring vane is bearing in device in the chamber or on the chamber, described stirring vane is moved back and forth under the effect of calutron.
3. method according to claim 2, wherein: be used for the device that this stirring vane is bearing in the chamber or on the chamber is comprised a pair of arm that stretches out from this liquid moving surface, this arm is positioned at a pair of slit, and in the sidewall that stretches out from pedestal, it has determined chamber to slit for this.
4. according to claim 2 or 3 described methods, also comprise: arrive photodetector by making light beam that optical transmitting set sends through described chamber and the opening (106) that is formed on the liquid moving surface of described stirring vane, thereby detect the analyte in described sample.
5. method according to claim 4, wherein: sample is a GH, and detects between 405nm and 460nm by the spectrophotometric counter device.
6. a stirring vane (100) comprising: liquid moving surface and be used for described stirring vane is bearing in device in the chamber or on the chamber, thus stirring vane can be moved back and forth in this chamber.
7. stirring vane according to claim 6, wherein: be used for the device that stirring vane is bearing in the chamber or on the chamber is comprised a pair of arm that stretches out from the liquid moving surface.
8. according to claim 6 or 7 described stirring vanes, wherein: this stirring vane becomes T shape.
9. according to claim 6,7 or 8 described stirring vanes, wherein: this liquid moving surface has the opening (106) that is formed at wherein, and light beam can pass through this opening.
10. according to any one described stirring vane in the claim 6 to 9, wherein: this stirring vane comprises a magnetic material.
11. according to any one described stirring vane in the claim 6 to 9, wherein: this stirring vane comprises a piezoelectric.
12. a sample container comprises the chamber (3,5) that is used to install stirring vane (100), described stirring vane is installed in the described sample container or on the sample container, like this, this stirring vane can move back and forth in chamber.
13. sample container according to claim 12 comprises: a pedestal, this pedestal have the sidewall that extends from it, and to determine chamber, described sidewall comprises the device that supports this stirring vane.
14. sample container according to claim 13, wherein: the device of described this stirring vane of supporting is a pair of slit in described sidewall.
15. according to any one described sample container among the claim 12-14, wherein: described chamber is an optical cell.
16. according to any one described sample container among the claim 12-15, wherein, this container is carousel or box.
17. carousel according to claim 16 is used to chemically examine, wherein, sample is separated into first and second portion, these parts will give instrument, and this device comprises: first import, this first import are or lead to collection chamber of first; Second import, this second import are or lead to second portion collection chamber; And an inlet hole, this inlet hole is equipped with filter for installation or bonding agent holding device, described inlet hole can be with respect to each described first import and second import and is moved, like this, inlet hole can be as required successively with each first and second import fluid connection.
18. sample container according to claim 16, this sample container is a carousel, comprising: a base part, this base part have a plurality of chambers that comprise first and second imports; A top section, this top section forms carousel with base part; And a funnel part, this funnel part comprises an inlet hole, described carousel is rotatably installed around described funnel part.
19. an instrument that is used to install sample container, this sample container comprise a chamber that is used to install stirring vane, described instrument comprises the device that described stirring vane is moved back and forth in this chamber.
20. device according to claim 19, wherein: the described device that is used to described stirring vane is moved back and forth is a calutron.
21. device according to claim 20, wherein: described calutron is a solenoid.
22. equipment, the instrument that comprises an analyte in the sample that can detect in sample container, this sample will give this instrument, this sample container comprises a chamber that is used to install stirring vane, described stirring vane be installed in the described chamber or chamber on, like this, described stirring vane can move back and forth when being driven by described instrument.
23. an equipment comprises an instrument and an a kind of device that is used for these one or more samples are given instrument that is used to read one or more samples, wherein, one or more samples is positioned to read the position be to utilize two stage identification to obtain.
24. equipment according to claim 23, wherein: first switch sends the information of the scope of living in of this device to this instrument, and second switch is confirmed accurately alignment.
25. according to claim 23 or 24 described equipment, wherein: excite by " element " on device in first micro switch on the instrument, this constitutes the phase I of detecting, second switch on the instrument plays the effect of " fine setting ", and is excited when instrument arrives the accurate position of this instrument.
26. equipment according to claim 25, wherein: " element " on device is a protruding part, and this protruding part forces down the plate that micro switch is installed by the rocker arm assembly.
27. device according to claim 26, wherein: two parts of switch are groove and elastic component on instrument or the elastic arms on the outermost sidewall of device.
28. device according to claim 27, wherein: this device is carousel or box type device.
29. device according to claim 28 also comprises: the switch of the 90 ° of distributions in four intervals.
30. a method that is used for determining blood glycosylation percentage, this method comprises: blood sample is divided into includes one or more not firsts and the second portion that includes one or more glycosylated proteins of glycosylated protein; Between 405nm and 460nm, GH is carried out detected/quantified by the spectrophotometric counter device.
31. method according to claim 30, wherein: the detected/quantified of GH is measured at about 440nm.
32. a device comprises one or more capillary devices that are used for stranded blob, leaves the second portion of first and access to plant so that guarantee it.
33. device according to claim 32, wherein: this device comprises a web or a like between first and second parts.
34. according to claim 32 or 33 described devices, wherein: this device is such type, be that inlet hole can move with respect to each first and second import, this first and second import is or leads to first and second collection chamber, described inlet hole is an infundibulate, and filter for installation or bonding agent holding device are housed, the outlet of described funnel is crossed in the position of described web.
35. an equipment, this equipment comprise a kind of instrument and a kind of device that is used for these one or more samples are given instrument that is used to read one or more samples, wherein, this device firmly is held in place in this instrument by spring clip.
CN00808712A 1999-06-10 2000-06-09 Mixing apparatus and method of mixing Pending CN1384769A (en)

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GBGB9913560.0A GB9913560D0 (en) 1999-06-10 1999-06-10 Mixing apparatus and method of mixing

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101498717A (en) * 2005-04-30 2009-08-05 艾康生物技术(杭州)有限公司 Sample collecting device used for collecting solid or semi-solid sample
CN1760672B (en) * 2004-10-12 2010-05-12 因韦尔尼斯医药瑞士股份有限公司 Device for detecting sample and method for detecting sample
CN103217321A (en) * 2012-05-17 2013-07-24 常州市环境监测中心 Drum type rotation instrument
CN108613932A (en) * 2018-07-18 2018-10-02 无锡迅杰光远科技有限公司 A kind of new side illuminated sample mixing device
CN108705670A (en) * 2018-05-03 2018-10-26 芜湖凯德机械制造有限公司 A kind of dispenser of mixed ingredients
CN109513364A (en) * 2017-09-18 2019-03-26 捷特生物制药有限公司 Mixer head with identification device
CN110398595A (en) * 2018-04-24 2019-11-01 深圳市帝迈生物技术有限公司 A kind of blood sample automatic mixing device and blood cell analysis equipment
CN110907380A (en) * 2019-12-25 2020-03-24 安徽清大云博环保科技有限公司 Method for measuring trace silicate in water

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100798471B1 (en) * 2007-10-08 2008-01-28 주식회사 인포피아 Reaction cassette for measuring glycated hemoglobin and measuring method thereof
EP2349377B1 (en) * 2008-11-28 2017-04-05 Terumo Kabushiki Kaisha Blood bag system and cassette
WO2015017413A1 (en) * 2013-07-29 2015-02-05 Mbio Diagnostics, Inc. Assay cartridge processing systems and methods and associated assay cartridges

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3356346A (en) * 1966-01-03 1967-12-05 Landsberger Kurt Test tube stirring support
US3853010A (en) * 1973-03-05 1974-12-10 Varian Associates Sample container support with coding means
GB1595881A (en) * 1978-02-13 1981-08-19 Pye Electronic Prod Ltd Sample cell and stirrer for spectrophotometry
SU797750A1 (en) * 1978-04-06 1981-01-23 Государственный Союзный Заводпо Механической И Химическойочистке Котлоагрегатов Method and apparatus for ajitating substances
US4254083A (en) * 1979-07-23 1981-03-03 Eastman Kodak Company Structural configuration for transport of a liquid drop through an ingress aperture
CA1146769A (en) * 1980-02-04 1983-05-24 Beat E. Glatthaar Process for the determination of diabetes
US4407961A (en) * 1981-03-16 1983-10-04 Sanders James L Ion-exchange system and method for isolation and determination of glycosylated hemoglobin in human blood
US4533641A (en) * 1982-07-21 1985-08-06 Harbor Branch Foundation, Inc. Automatic chemical analysis methods
GB2125962B (en) * 1982-08-20 1986-02-05 Philips Electronic Associated Automatic sampling arrangement
US4612291A (en) * 1984-09-04 1986-09-16 American Monitor Corporation Method and apparatus for mixing serum and reagents for chemical analysis
GB8614084D0 (en) * 1986-06-10 1986-07-16 Serono Diagnostics Ltd Immunoassay
US5272092A (en) * 1987-11-12 1993-12-21 Hitachi, Ltd. Method for analyzing a reaction solution
US4990075A (en) * 1988-04-11 1991-02-05 Miles Inc. Reaction vessel for performing sequential analytical assays
DE8816086U1 (en) * 1988-12-24 1989-02-09 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad, De
US6156270A (en) * 1992-05-21 2000-12-05 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membranes
US5597532A (en) * 1994-10-20 1997-01-28 Connolly; James Apparatus for determining substances contained in a body fluid
WO1999028038A1 (en) * 1997-11-28 1999-06-10 Cortecs Diagnostics Limited Device and apparatus for conducting an assay

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1760672B (en) * 2004-10-12 2010-05-12 因韦尔尼斯医药瑞士股份有限公司 Device for detecting sample and method for detecting sample
CN101498717A (en) * 2005-04-30 2009-08-05 艾康生物技术(杭州)有限公司 Sample collecting device used for collecting solid or semi-solid sample
CN103217321A (en) * 2012-05-17 2013-07-24 常州市环境监测中心 Drum type rotation instrument
CN109513364A (en) * 2017-09-18 2019-03-26 捷特生物制药有限公司 Mixer head with identification device
CN110398595A (en) * 2018-04-24 2019-11-01 深圳市帝迈生物技术有限公司 A kind of blood sample automatic mixing device and blood cell analysis equipment
CN108705670A (en) * 2018-05-03 2018-10-26 芜湖凯德机械制造有限公司 A kind of dispenser of mixed ingredients
CN108613932A (en) * 2018-07-18 2018-10-02 无锡迅杰光远科技有限公司 A kind of new side illuminated sample mixing device
CN108613932B (en) * 2018-07-18 2023-08-22 无锡迅杰光远科技有限公司 Novel side-illuminated sample mixing device
CN110907380A (en) * 2019-12-25 2020-03-24 安徽清大云博环保科技有限公司 Method for measuring trace silicate in water

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BR0011444A (en) 2002-03-19
CA2374590A1 (en) 2000-12-21
WO2000076642A2 (en) 2000-12-21
IL147008A0 (en) 2002-08-14
KR20020016828A (en) 2002-03-06
GB9913560D0 (en) 1999-08-11
NZ516057A (en) 2003-08-29
WO2000076642A3 (en) 2001-07-05
JP2003501250A (en) 2003-01-14
AU5239100A (en) 2001-01-02
RU2001133017A (en) 2004-01-27
EP1185357A2 (en) 2002-03-13

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