CN1360024A - Consortive process for culturing bacillus thuringiensis - Google Patents
Consortive process for culturing bacillus thuringiensis Download PDFInfo
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- CN1360024A CN1360024A CN 00134344 CN00134344A CN1360024A CN 1360024 A CN1360024 A CN 1360024A CN 00134344 CN00134344 CN 00134344 CN 00134344 A CN00134344 A CN 00134344A CN 1360024 A CN1360024 A CN 1360024A
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- bacillus thuringiensis
- sorbose
- consortive
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A consortive method for preparing bacillus thuringiensis incldues such steps as mixing bacillus thuringiensis with oxidizing gluconic acid bacillus, culturing in the culture medium containing sorbose (1-2%) until that their number ratio is 1:10-20, inoculating to fermenting culture meidum containing sorbose (6-14%), and aerobic fermenting. Its advantages are high conversion of 2-keto-L-colombic acid and high yield of bacillus thuringiensis.
Description
The present invention relates to the new technology that a kind of Bacillus thuringiensis produces, promptly use two kinds of bacterium--Bacillus thuringiensis and oxidizing glucose acidfast bacilli mix with giving birth to technology, produce Bacillus thuringiensis.
Thuricade-1 is the widely used biotic pesticide of a class.Demand rises year by year since 1938 emerge, and annual sales amount has reached 1,000,000,000 dollars on this life circle.Along with the development of modern pollution-less agriculture, bigger growth will appear in its demand.For promoting the fast development of these biotic pesticide, people once studied the problems that restrict its development, and one of them key is to reduce cost.For many years, by strain improvement, the improvement of training method and culture condition, production cost is descending year by year, but amplitude is little.On the high side be a major obstacle that influences its fast development always.
Purpose of the present invention, promptly Bacillus thuringiensis is mixed with bacillus of oxidizing glucose with giving birth to and carried out systematic study at above-mentioned situation, find out their mutual effect and optimal reaction states in giving birth to together, determine production technique, in the ancient dragon acid of High-efficient Production 2-ketone group-L-, produce Bacillus thuringiensis, realize the Ultra Low Cost that Bacillus thuringiensis produces.
Major technique feature of the present invention is: select Bacillus thuringiensis and mix with oxidizing glucose acidfast bacilli 1: 20～30, in containing 1～2% sorbose seed culture medium, vibration is 1/10～20 with giving birth to 20～24 hours ratios to two bacterium bacterium numbers of cultivation under 28～33 ℃ of conditions, be connected to 10～15% inoculum sizes that to contain sorbose be in 6～14% the fermentation culture, aerobic fermentation 48～60 hours is reduced to below the 1mg/ml to sorbose content under 28～33 ℃ condition, and the Bacillus thuringiensis gemma reaches 10
9～10
11Individual/ml, fermentation clear liquid changes 2-ketone group-L-extraction technology for cologne acid over to from going out thalline, and residue is dried to the commodity pulvis.
The present invention mixes with the oxidizing glucose acidfast bacilli with giving birth to by Bacillus thuringiensis, in good condition biotic pesticide and the sour two class productions of the ancient dragon of 2-ketone group-L-are combined together, can make ten thousand tons of vitamins C two stage fermentation factory every year from the ancient imperial sour yield of 2-ketone group-L-improves, synergy 2000～2,500 ten thousand yuan make a profit 1,000 ten thousand from the Bacillus thuringiensis of output.Economic benefit is big, society and obvious environment benefit.
Embodiment
1. respectively picking Bacillus thuringiensis B.tsp.529 and oxidizing glucose acidfast bacilli contained in the 2% sorbose nutrient solution in 3 liters by 1: 20, and shaking culture is 24 hours under 29 ℃ of conditions, changes 0.5M over to
3(include 300 liter of 2% sorbose seed culture fluid) in the seeding tank, being cultured to two bacterium bacterium under 29 ℃ of conditions, to count ratio be 1/10～20, inserts 4M
3(include 2800 liter of 10% sorbose fermentation culture) in the fermentor tank, 29 ℃ of aerobic fermentations 52 hours, sorbose is reduced to 0.68mg/ml.The ancient imperial sour transformation efficiency of 2-ketone group-L-reaches 88.65%, and contrast combination fungus strain improves 6.4 percentage points, the Bacillus thuringiensis gemma 3.2 * 10 of generation
10Individual/ml, reach the spore amount that Bacillus thuringiensis forms in common starch culture-medium.The preparation as insecticiding effect is obvious, and it is strong to use adaptability.
2. select Bacillus thuringiensis B.tsp.203 to mix with the oxidizing glucose acidfast bacilli, pressed above-mentioned aerobic fermentation 48 hours, sorbose is reduced to 0.32mg/ml.The ancient imperial sour transformation efficiency of 2-ketone group-L-reaches 85.50%, and contrast combination fungus strain improves 3.2 percentage points, the Bacillus thuringiensis gemma 3.9 * 10 of generation
10Individual/ml.
3. select Bacillus thuringiensis B.tsp.553 to mix with the oxidizing glucose acidfast bacilli, pressed above-mentioned aerobic fermentation 48 hours, sorbose is reduced to 0.61mg/ml.The ancient imperial sour transformation efficiency of 2-ketone group-L-reaches 86.44%, and contrast combination fungus strain improves 4.2 percentage points, the Bacillus thuringiensis gemma 3.5 * 10 of generation
10Individual/ml.
Claims (1)
1. Consortive process for culturing bacillus thuringiensis, it is characterized in that selecting Bacillus thuringiensis to mix with oxidizing glucose acidfast bacilli 1: 20～30, in containing 1～2% sorbose seed culture medium, vibration is 1/10～20 with giving birth to 20～24 hours ratios to two bacterium bacterium numbers of cultivation under 28～33 ℃ of conditions, be connected to 10～15% inoculum sizes that to contain sorbose be in 6～14% the fermentation culture, aerobic fermentation 48～60 hours is reduced to below the 1mg/ml to sorbose content under 28～33 ℃ condition, and the Bacillus thuringiensis gemma reaches 10
9～10
11Individual/ml, fermentation clear liquid changes the ancient imperial acid extraction operation of 2-ketone group-L-over to, and residue is dried to the commodity pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00134344 CN1360024A (en) | 2000-12-21 | 2000-12-21 | Consortive process for culturing bacillus thuringiensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00134344 CN1360024A (en) | 2000-12-21 | 2000-12-21 | Consortive process for culturing bacillus thuringiensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1360024A true CN1360024A (en) | 2002-07-24 |
Family
ID=4596177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00134344 Pending CN1360024A (en) | 2000-12-21 | 2000-12-21 | Consortive process for culturing bacillus thuringiensis |
Country Status (1)
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| CN (1) | CN1360024A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003016508A3 (en) * | 2001-08-15 | 2003-10-02 | Cerestar Holding Bv | Process for the manufacture of 2-keto-l-gulonic acid |
| CN101603060B (en) * | 2009-07-10 | 2011-06-01 | 天津大学 | Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid |
| CN102978273A (en) * | 2012-11-13 | 2013-03-20 | 中国科学院沈阳应用生态研究所 | Method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid |
-
2000
- 2000-12-21 CN CN 00134344 patent/CN1360024A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003016508A3 (en) * | 2001-08-15 | 2003-10-02 | Cerestar Holding Bv | Process for the manufacture of 2-keto-l-gulonic acid |
| US7091013B2 (en) | 2001-08-15 | 2006-08-15 | Cerestar Holding B.V. | Process for the manufacture of 2-keto-L-gulonic acid |
| CN101603060B (en) * | 2009-07-10 | 2011-06-01 | 天津大学 | Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid |
| CN102978273A (en) * | 2012-11-13 | 2013-03-20 | 中国科学院沈阳应用生态研究所 | Method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid |
| CN102978273B (en) * | 2012-11-13 | 2014-01-08 | 中国科学院沈阳应用生态研究所 | Method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |