CN1322952A - Electronic counting method and counter for physical body fluid components - Google Patents
Electronic counting method and counter for physical body fluid components Download PDFInfo
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- CN1322952A CN1322952A CN 01115783 CN01115783A CN1322952A CN 1322952 A CN1322952 A CN 1322952A CN 01115783 CN01115783 CN 01115783 CN 01115783 A CN01115783 A CN 01115783A CN 1322952 A CN1322952 A CN 1322952A
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- body fluid
- visible component
- microlitre
- computing machine
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Abstract
After calibrating the pixel number in a practical length, or calibration coefficient, with calibrating matter and further determining one check with specific side length and the pixel number corresponding to the side length, the pixel number is stored in computer as measuring and counting standard. Counted body fluid is fed, and physical component number in several continuous images is read out and the number of physical component each microliter may be obtained after proper conversion. The counter device with the specific software is also discloses.
Description
The present invention relates to body fluid visible component electronic counting method and device thereof that clinical osology composition detects usefulness.
It is to use microslide that traditional clinical osology detects, during detection, body fluid is coated on the microslide, be pressed on the microslide with cover glass again, place on the microscope stage then, by 10-20 visible component within the vision of low power or high power microscopy counting, the average of the visible component of seeing with every low power or every high power is reported the result at last.This pick-up unit has advantage simple in structure, easy to use, and its shortcoming is that accuracy is low.
The device that the clinical osology that is used for another kind detects is a counting chamber, and for example, the applicant is disclosed in the Chinese patent application 00264799.0 on Dec 25th, 2000.Detecting with counting chamber is that body fluid is charged in the counting chamber, places then on the microscopical articles holding table, treats the static back of body fluid reading.Counting chamber be a height uniformity (for example, 0.1mm), and the tube chamber of surperficial tool scale, therefore, as long as the visible component in the certain grid of counting promptly can every microlitre or milliliter in a visible component number report the result.Obviously, counting chamber has than higher reliability and the repeatability of microslide detection, can reflect the quantity of visible component in the body fluid objectively.
But actually, some visible components are difficult in and are deposited as suspended state in the sample.See it clearly, must could realize by focusing on.On the other hand, the depth of focus of microscope itself is very short, adopts counting chamber to detect, and it is that visible component on the different levels in the 0.1mm suspends that microscope will be observed the degree of depth, scale just occurred seeing clearly, but has not seen the problem of visible component.
Therefore, the object of the present invention is to provide a kind of body fluid visible component electronic counting method that clinical osology visible component detects that is used for.
Another object of the present invention is to provide a kind of body fluid visible component digital electronic unit that clinical osology visible component detects that is used for.
According to body fluid visible component electronic counting method of the present invention, comprise the steps:
At first obtain tested body fluid, standby.Described body fluid, for example, urine.Detect content, as the cast in the urine.
The required device of this method is finished in connection, comprising: computing machine and external device (ED) thereof, and for example, printer contains the microscope of CCD, and the device of tested body fluid is provided, for example, the arena detector.
Calibration, with a slide that contains accurate calibration, for example, microscope calibration differential chi places on the microscope stage, regulates microscope, can clear demonstration on display screen until the calibration thing.Write down the physical length L of this section calibration thing, and the number of picture elements P under this physical length, advance then to be calculated as follows calibration coefficient K,
K=P/L for example, physical length L is 200 microns, contained number of picture elements P is 180, thus, calibration coefficient K=0.9 pixel/micron determining 1 micron contained number of picture elements, and will calibrate the thing image and calibrate the result and deposit computing machine in.
Fix, purpose is to determine a specific length of side L
0Grid so that the actual count volume V of the significant height H that passes through according to counting chamber body fluid is a certain value, promptly
L
0=[V/H]
1/2, because the height H that the effective body fluid of counting chamber flows is 0.1 millimeter, actual count volume V
0Be typically chosen in laboratory examination and report some/one of the contained body fluid visible component of every microlitre number, so that calculate.For example, the actual count volume is chosen as 0.025 milliliter, therefore,
L
0=[0.025/0.1]
1/2=0.5 millimeter corresponding on display the pixel value P of the drawn foursquare length of side
0For
P
0=KL
0As by K value as described in the scaling step example, P
0=0.9 * 500=450 pixel.Thus, determine the contained number of picture elements of every lattice, and deposit in the computing machine;
With aforementioned body fluid by the flowing counting pool on the body fluid generator input microscope stage;
Visible component number in the counting, continuous counter n width of cloth dynamic image grid is got suitable several m, to obtain visible component number R in every microlitre volume, that is,
R=nmv, for example, by previous embodiment, the V=0.025 microlitre is got n=8, can draw the visible component of per 0.2 microlitre, then, gets suitable several m=5, just changes into the contained visible component number of every microlitre.
If the visible component number is more in every lattice, be not easy to counting, at this moment body fluid is diluted, for example, dilution w doubly, under these circumstances, the result of counting gained also need multiply by extension rate, at this moment resultingly is only every microlitre and contains and form a part number, promptly
R=wnmV。
The contained visible component number of every microlitre that obtains is come out with printer prints.
According to body fluid visible component digital electronic unit of the present invention, comprise the device that tested body fluid is provided, contain the microscope of CCD, computing machine and servicing unit thereof is characterized in that also comprising the software of aforementioned method of counting.
Obviously, compare, have the following advantages according to body fluid visible component electronic counting method of the present invention and device thereof and prior art:
The first, counting chamber does not need groove, thereby has saved cost.
The second, the calibration thing is easy to get.
The 3rd, in case after hardware configuration is fixed, only need once calibration to use for a long time, no
Must calibrate repeatedly.
The 4th, testing result accurately and reliably.
The 5th, alleviate operator's labour intensity, easy to operate.
Above-mentioned purpose of the present invention and other purpose and advantage thereof will be more readily apparent from by the detailed description below in conjunction with the accompanying drawing illustrated embodiment, and accompanying drawing has:
Fig. 1 is the composition frame chart according to an embodiment of body fluid visible component digital electronic unit of the present invention;
Fig. 2 is the composition frame chart of the tested body fluid generator in the device shown in Figure 1;
Fig. 3 is the process flow diagram according to the specific software of body fluid visible component digital electronic unit of the present invention.
Fig. 1 shows the composition frame chart according to an embodiment of body fluid visible component digital electronic unit of the present invention, and this embodiment is a digital electronic unit that detects the visible component in the arena.As shown in the figure, it comprises that one provides 1, one of the urine sediment analyzer of tested urine sample to be with 2, one in the microscope of CCD to contain computing machine 3 and a printer 4 of specific software 5.The wherein said urine sediment analyzer 1 that tested urine sample is provided as shown in Figure 2.Urine sediment analyzer 1 comprises a device 11 and a device 12 of quantitatively drawing dyeing liquor of quantitatively drawing urine sample, drawing urine sample and dyeing liquor mixes in mixer 13, in other words, the detection urine sample that present embodiment provides is through dyeing, thereby overcome the shortcoming of do not dye sediment mirror flase drop, omission, improved degree of detecting.Urine sample through mixed dyeing is sent into flowing counting pool 14 by mixer 13.According to flowing counting pool of the present invention be one not with the flowing counting pool of scale.Flowing counting pool is to place on the objective table of the microscope 2 of being with CCD, the user can be by the visual system on the microscope 2, perhaps on the display of computing machine 3, read the number of the visible component in the urine by CCD, and the computed in software of explanation is gone out the visible component number of every microlitre, and then print examining report by printer 4 by the back.After detecting end, clean, by tapping equipment 16 cleaning fluid (containing urine sample) is discharged then, for next urine examination is got ready by 15 pairs of flowing counting pools 14 of cleaning device.
If find that in detection the visible component amount is excessive in the urine sample, be not easy to detect, at this moment carry out doubling dilution by 17 pairs of urine samples of doubling dilution device, import flowing counting pool 14 then until obtaining suitable detection urine sample, and the multiple of dilution is imported computing machine 3.
The specific software of computing machine 3 is described referring now to Fig. 3.This software comprises:
The image of selected calibration thing and every micron contained number of picture elements determining by the calibration thing, i.e. calibration coefficient K;
A length of side is L
0Grid, make high for the actual count volume of H be V;
L
0=[V/H]
1/2The number of picture elements P of this length of side
0For:
R
0=KL
0The width of cloth of continuous counter dynamic image grid is counted n, and selected suitable several m also import computing machine; Calculate the number R of the visible component of every microlitre
R=nmV。
According to one embodiment of the present of invention, described software also comprises body fluid extension rate w, and the number R of corresponding visible component is:
R=wnmV。
According to another embodiment of the present invention, H=0.1 millimeter wherein, V=0.025 microlitre, L0=0.5 milliliter, n=8, m=5.
The applicant makes detailed description with regard to design of the present invention and embodiment; the professional and technical personnel makes various conversion and improvement on this basis; but these conversion and improvement all do not break away from spirit of the present invention, all within the protection domain that claims limited.
Claims (8)
1. the electronic counting method of a body fluid visible component may further comprise the steps:
Obtain humoral sample;
Open the microscope of body fluid generator, band CCD, computing machine and printer;
To calibrate thing and place the microscope objective table, focusing deposits at computing machine until the calibration thing;
On the figure of calibration thing, get a line segment L, and write down the contained number of picture elements P of this line segment, calculate calibration coefficient K,
K=P/L, and deposit computing machine in;
Setting up a length of side is L
0Grid, make high for the actual count volume of H is V, therefore, described length of side L
0For:
L
0=[V/H]
1/2The number of picture elements P of this length of side
0For:
P
0=K * L
0And deposit computing machine in;
Described humoral sample is placed flowing counting pool on the microscope stage by body fluid generator input;
Visible component number in the continuous counter n width of cloth dynamic image grid is got suitable several m, with the number R of the visible component that obtains every microlitre,
R=nmV
0
Print the number of every microlitre visible component.
2. in accordance with the method for claim 1, it is characterized in that this method comprises that also the multiple of its dilution is W to the step of humoral sample dilution, therefore, the number R of the effective ingredient of corresponding every microlitre is:
R=wnmV
0。
3. in accordance with the method for claim 1, it is characterized in that described counting chamber height H=0.1 millimeter, the counting volume is 0.025 microlitre, length of side L
0=0.5 millimeter, the width of cloth of the dynamic image grid of continuous counter is counted n=8, suitably counts m=5.
4. in accordance with the method for claim 1, it is characterized in that described body fluid is urine, described body fluid generator is a urine sediment analyzer.
5. a body fluid visible component digital electronic unit comprises a body fluid generator, the microscope of band CCD, and computing machine and printer is characterized in that described computing machine contains a specific software, this software comprises:
The image of selected calibration thing and every micron contained number of picture elements P, i.e. calibration coefficient K determining by the calibration thing;
A length of side is L
0Grid, make high for the actual count volume of H is V,
L
0=[V/H]
1/2The number of picture elements P of this length of side
0For:
P
0=K×L
0；
The width of cloth of the dynamic figure grid of continuous counter is counted n, suitable several m,
Calculate the number R of the visible component of every microlitre,
R=nmV。
6. according to the described device of claim 5, it is characterized in that described specific software also comprises body fluid extension rate W; The number R of corresponding visible component is:
R=wnmV。
7. according to the described device of claim 5, it is characterized in that the H=0.1 millimeter, V=0.025 microlitre, L0=0.5 millimeter, n=8, m=5.
8. according to the described device of claim 5, it is characterized in that described body fluid is urine, described body fluid generator is a urine sediment analyzer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115783 CN1126954C (en) | 2001-07-02 | 2001-07-02 | Electronic counting method and counter for physical body fluid components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115783 CN1126954C (en) | 2001-07-02 | 2001-07-02 | Electronic counting method and counter for physical body fluid components |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1322952A true CN1322952A (en) | 2001-11-21 |
| CN1126954C CN1126954C (en) | 2003-11-05 |
Family
ID=4662196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01115783 Expired - Fee Related CN1126954C (en) | 2001-07-02 | 2001-07-02 | Electronic counting method and counter for physical body fluid components |
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|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8904208B2 (en) | 2011-11-04 | 2014-12-02 | International Business Machines Corporation | Run-time task-level dynamic energy management |
| CN105866123A (en) * | 2016-04-29 | 2016-08-17 | 李少军 | Biological cell sap test box provided with filter membrane |
-
2001
- 2001-07-02 CN CN 01115783 patent/CN1126954C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8904208B2 (en) | 2011-11-04 | 2014-12-02 | International Business Machines Corporation | Run-time task-level dynamic energy management |
| US9021281B2 (en) | 2011-11-04 | 2015-04-28 | International Business Machines Corporation | Run-time task-level dynamic energy management |
| CN105866123A (en) * | 2016-04-29 | 2016-08-17 | 李少军 | Biological cell sap test box provided with filter membrane |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1126954C (en) | 2003-11-05 |
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