CN1213497A - Germfree fly and its pupa prodn. method - Google Patents

Germfree fly and its pupa prodn. method Download PDF

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Publication number
CN1213497A
CN1213497A CN 97118970 CN97118970A CN1213497A CN 1213497 A CN1213497 A CN 1213497A CN 97118970 CN97118970 CN 97118970 CN 97118970 A CN97118970 A CN 97118970A CN 1213497 A CN1213497 A CN 1213497A
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fly
box
egg
larva
incubating
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司信
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Abstract

A method for raising abacterial fly and fly maggots includes raising the housefly until the third generation, raising the third-generation housefly in cage until it oviposits, incubating the ova to obtain maggots, raising the maggots, separating the maggots out in separating box at 25-38 deg.C and 60-80% of relative humudity, and drying, and features abacterial raising, no escape of maggots, full separation, and high oviposit rate and 100% of inculating rate at 35 deg.C and 65-70% of relative humidity.

Description

The production method of aseptic fly and fly maggot
At insect world, the fertility of fly ranks first place, and a pair of fly can breed 2,660 hundred million fly maggots in four months, and weight can reach more than 600 tons.With aseptic fly and aseptic fly-maggot is the feedstuff that animal albumen powder that primary raw material is made is made poultry and livestock, can promote and quicken growth, the growth of poultry and livestock.Contain because of aseptic fly and fly maggot and plant trace element, lower fat surplus 18 seed amino acids and ten of more animal protein and needed by human body, thereby also can develop human good food, health products or medicine.Yet do not see the production technology of announcing aseptic fly and fly maggot so far as yet, influence the development and use of aseptic fly and fly maggot.
The objective of the invention is to invent the production technology of a kind of aseptic fly and aseptic fly-maggot.
Step of the present invention is as follows:
1, select housefly for cultivating the fly kind of culturing: the fly kind that China has found at present has kind more than 1500, population quantity maximum wherein, and fertility is the strongest, and movable season, the longest fly kind was a housefly, and therefore selecting housefly is the fly kind of cultivating and culturing.
2, cultivate no bacterial classification fly from the housefly of occurring in nature.Method is that the egg-incubating box that will be placed with egg-incubating material is placed on the place that the natural world housefly multiplies, after treating that housefly lays eggs, it is 25-38 ℃ that egg-incubating box is placed on temperature, relative moisture is to hatch into larva under the environment of 60-80%, larva is put into thimerosal soak a moment, pull the back out and use normal saline flushing, use distilled water flushing again, pupate then, be placed on the cage back warp again and spend the adult stage and cultivate into second generation housefly.Cultivate third generation housefly with the second generation housefly that cultivates with said method again.Instrument that nurturing period is used and equipment are wanted to use after the strict sterilization again.The third generation housefly that evidence is cultivated in this way guarantees aseptic.
3, the kind fly that will cultivate is raised in breeding cage and lays eggs.Breeding cage is made up of gauze, the framework that supports gauze and airtight base plate.The hole of feeding is left in the side of breeding cage.The hole of feeding is stitched with cloth sleeve.Be placed with trough, drinking trough and the groove of laying eggs in the breeding cage, be placed with kind of a fly feed in the trough, be placed with a clear water and a small piece of foam in the drinking trough, stop the suction that falls, put egg-incubating material in the groove of laying eggs so that plant fly.Material of trough, drinking trough and lay eggs groove and the inside and water are changed once every day.The hole of feeding is to put fly, get ovum, change the import and export of raising tool and feed.It is 25-38 ℃ that breeding cage is placed on temperature, and relative moisture is in the environment of 60-80%.For the ease of environment is controlled, breeding cage preferably is placed in the receptacle, so that can both produce throughout the year.Receptacle can be installed fluorescent lamp, so that promote the nocturnalism of kind of fly.In order more effectively to utilize the receptacle space, be preferably in the raising frame of putting multilayer in the receptacle, breeding cage is placed on raises on the frame.Temperature adjustment, damping equipment and temperature and humidity monitoring instrument are arranged in the receptacle.
4, fly blow cultivated into larva in egg-incubating box, be fly maggot.Ovum and egg-incubating material in the groove of laying eggs that will every day takes out in the breeding cage are poured in the egg-incubating box, add the egg-incubating material of appropriate amount again, and making fly blow is that 25-38 ℃, relative moisture are to hatch into larva under the 60-80% environment naturally in temperature.For the ease of temperature and humidity is controlled, egg-incubating box should be placed in the brood chamber equally.Egg-incubating box is made by airtight material, above opening.In order to prevent that larva from climbing out of, egg-incubating box is placed on by educating in the outer box of ovum that lubricious material is made, and the outer wall of egg-incubating box is nibbled to be merged into the inwall of educating the outer box of ovum and is slidingly matched, and educates the outer box of ovum and is higher than egg-incubating box.
5, the larva in the egg-incubating box and egg-incubating material are poured in the Seperating box separate.Seperating box is sieve shape thing, and the side is airtight sheet material, upper shed, and the bottom is a net.Connect the larva of putting an airtight upper shed below the Seperating box and collect box.According to the characteristics of larva, larva specially climbs in dark, falls into larva and collects the box so can be automatically get out from the mesh of Seperating box bottom.Disengaging time is approximately 0.5-1 days, will constantly stir larva and egg-incubating material in the Seperating box around here, to accelerate separation.
6, isolated larva is carried out drying and handle, make dried maggot.
7, the kind fly in the breeding cage is replenished.Because the life-span of housefly was generally about 20 days, when the kind fly quantity in finding breeding cage reduces, then need to replenish.Method is that isolated larva is placed in the box of pupating, and the box of will pupating is again put in the breeding cage and pupated, sprouts wings into fly.
This method is that living condition, habit and the characteristics according to housefly are invented.Can guarantee and promote growth, growth and the breeding of housefly.It is aseptic to adopt cage system to guarantee, and it is concentrated to lay eggs.The setting of educating the outer box of ovum can guarantee that fly maggot do not flee.The separating method that adopts can guarantee to separate clean, thorough, and is automatic separation.If temperature and humidity is controlled at optimum state, can makes kind of fly egg laying amount reach incubation rate maximum, fly blow and reach 100%
The inventor has used two houses, an intercropping kind fly receptacle, another intercropping brood chamber, every be of a size of long * wide * high=5 * 3 * 3 meters.Receptacle is used and is raised frame and place long * wide * high=40 of breeding cages of 1 * 0.8 * 0.7 meter.Each breeding cage is raised ten thousand kind flies of 3-4, the indoor fluorescent lamp that has 100 watts.Educate the outer box of ovum and make with glass, that inwall is of a size of is long * wide * high=0.8 * 0.5 * 0.3 meter, and egg-incubating box, Seperating box, larva are collected box and all make with timber, and that the inwall size is all is long * wide * high=0.78 * 0.48 * 0.2 meter.The upper limb of educating the outer box of ovum is controlled at 38-40 ℃ with temperature controller with the upper limb local temperature, stops action when fly maggot surpasses 40 ℃, guarantees further that with this way fly maggot do not flee.The prescription of planting the fly feed is sugar, milk powder, egg liquid, and proportioning is 1: 1: 1.The amount of giving of feed is 1.5 milligrams of every every days.The prescription of egg-incubating material is wheat bran, milk powder, egg liquid, and proportioning is that wheat bran 100 gram, milk powder 3-5 gram, water are an amount of, and to hold water outlet but flowing water does not drip and is as the criterion, and this amount is supported 500 fly maggots.The temperature of receptacle and brood chamber is controlled with the electric heater of automatic temperature-control, monitors with thermometer, and humidity is controlled with electric moistening device, monitors with moisture meter.
The inventor has made test of many times to the scope of temperature and humidity, learn when temperature at 35 ℃, humidity during at 65-70%, the egg laying amount of planting fly is the highest, the incubation rate of ovum can reach 100%, 20 day and produce 17 tons of dried maggots.When temperature respectively at 25 ℃, 38 ℃, relative moisture is respectively at 60%, 80% o'clock, the egg laying amount of planting fly is minimum, the incubation rate of ovum is also minimum, dried maggot output reduces by 50%.

Claims (10)

1, the production method of aseptic fly and fly maggot, it is characterized in that selecting housefly is to cultivate the fly kind of culturing; From natural housefly, cultivate no bacterial classification fly; The no bacterial classification fly of cultivating is raised in breeding cage and laid eggs; Fly blow is cultivated into larva in egg-incubating box; The larva of cultivating is separated with egg-incubating material in Seperating box; Isolated larva is carried out the drying processing make dried maggot; Kind fly in the breeding cage is replenished, and no bacterial classification fly is cultivated, the kind fly raises, lays eggs and the hatching of fly blow, the cultivation of larva and the environmental temperature of separation are 25-38 ℃, and relative moisture is 60-80%.
2, the production method of aseptic fly according to claim 1 and fly maggot, it is characterized in that from the method that the housefly of occurring in nature is cultivated no bacterial classification fly it being earlier the egg-incubating box that is placed with egg-incubating material to be placed on the place that housefly multiplies, after treating that housefly lays eggs, in egg-incubating box, hatch into larva, then larva being put into thimerosal soaks, pull the back normal saline flushing out, use distilled water flushing again, be put into after pupating and sprout wings into second generation housefly in the cage, and then cultivate third generation housefly with this method with the second generation housefly of cultivating, third generation housefly is done no bacterial classification fly.
3, the production method of aseptic fly according to claim 1 and fly maggot, it is characterized in that trough, drinking trough are arranged in the breeding cage, the groove of laying eggs, planting fly feed, clear water and foamed plastics, egg-incubating material is placed on respectively in trough, drinking trough and the groove of laying eggs, the hole of feeding on the breeding cage, cloth sleeve is sewed up in the hole of feeding, and material of trough, drinking trough, lay eggs groove and the inside and water are changed once by the hole of feeding every day.
4, the production method of aseptic fly according to claim 1 and fly maggot, it is characterized in that be that fly blow and the egg-incubating material that take out in the breeding cage every day are poured in the egg-incubating box with fly blow in the method for cultivating into larva in the egg-incubating box, the egg-incubating material that adds appropriate amount again, make fly blow hatch into larva, egg-incubating box is placed on by educating in the outer box of ovum that lubricious material is made, the outer wall of egg-incubating box is nibbled to be merged into the inwall of educating the outer box of ovum and is slidingly matched, and educates the outer box of ovum and is higher than egg-incubating box.
5, the production method of aseptic fly according to claim 1 and fly maggot, the method that the larva that it is characterized in that cultivating separates with egg-incubating material in Seperating box be with Seperating box make upper shed, all around airtight, below be the sieve shape thing of silk screen, connect the larva of putting an airtight upper shed below the Seperating box and collect box, the larva that the mesh of larva below Seperating box climbs out of below falling into is automatically collected in the box, disengaging time 0.5-1 days, look material and larva that feelings are stirred Seperating box the inside around here.
6, the production method of aseptic fly according to claim 1 and fly maggot is characterized in that to the method that the kind fly in the breeding cage is replenished it being that isolated larva is placed in the box of pupating, and the box of will pupating is again put into and pupated in the breeding cage and sprout wings into fly.
7, the production method of aseptic fly according to claim 4 and fly maggot is characterized in that the upper limb of educating the outer box of ovum is controlled at 38-40 ℃ with temperature controller with the upper limb local temperature.
8, according to the production method of claim 3 and 4 described aseptic flies and fly maggot, it is characterized in that kind of the prescription of fly feed is sugar, milk powder, egg liquid, proportioning is 1: 1: 1, the amount of giving of planting the fly feed is 1.5 milligrams of every every days, the prescription of egg-incubating material is wheat bran, milk powder, water, and proportioning is wheat bran 100 grams, milk powder 3-5 gram, water in right amount to hold water outlet but not flowing water drip and be as the criterion, this dosage is supported 500 larvas.
9, the production method of aseptic fly according to claim 5 and fly maggot is characterized in that the silk screen of Seperating box bottom is 30 purpose stainless steel cloths.
10, the production method of aseptic fly according to claim 1 and fly maggot, it is characterized in that kind of fly raise and lay eggs, the environmental temperature of the separation of the cultivation of the hatching of fly blow and larva, larva is that 35 ℃, relative moisture are 65-70%.
CN 97118970 1997-10-08 1997-10-08 Germfree fly and its pupa prodn. method Pending CN1213497A (en)

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CN 97118970 CN1213497A (en) 1997-10-08 1997-10-08 Germfree fly and its pupa prodn. method

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Application Number Priority Date Filing Date Title
CN 97118970 CN1213497A (en) 1997-10-08 1997-10-08 Germfree fly and its pupa prodn. method

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000074478A1 (en) * 1999-06-08 2000-12-14 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining a secretion from fly larvae for therapeutic application
CN100415092C (en) * 2005-07-29 2008-09-03 陶志伦 Method for catching fly maggot, and maggot catcher
CN101984850A (en) * 2010-12-03 2011-03-16 广东省昆虫研究所 Housefly adult feed
CN102550495A (en) * 2012-03-05 2012-07-11 厦门联南强生物环保科技有限公司 Method for culturing and domesticating fly species
CN102550487A (en) * 2011-01-21 2012-07-11 中国人民解放军第四五四医院 Production method of aseptic maggots
CN103798191A (en) * 2012-11-12 2014-05-21 安徽师范大学 Method for preventing and controlling waxberry orchard fruit flies by using housefly pupas to replace fruit fly pupas for breeding parasitic wasps
US8821896B2 (en) 2009-01-16 2014-09-02 Sanaria Inc. Purified Plasmodium and vaccine composition
CN104472441A (en) * 2014-12-04 2015-04-01 桐乡市恒易生物科技有限公司 Feeding method of hylemyia platura meigen
US9278125B2 (en) 2011-05-11 2016-03-08 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
US9764016B2 (en) 2013-01-25 2017-09-19 Sanaria Inc. Genetic attenuation of Plasmodium by B9 gene disruption
CN108770789A (en) * 2018-02-11 2018-11-09 宁波七禾加百列农业科技有限公司 Fly pieces of an egg hatch material and its hatching method
CN108935343A (en) * 2018-08-10 2018-12-07 陈希月 A kind of method and maggot bud albumen powder of artificial feeding maggot bud

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU777660B2 (en) * 1999-06-08 2004-10-28 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining a secretion from fly larvae for therapeutic application
US6863022B2 (en) 1999-06-08 2005-03-08 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining secretion from fly larvae for therapeutic application
WO2000074478A1 (en) * 1999-06-08 2000-12-14 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining a secretion from fly larvae for therapeutic application
CN100415092C (en) * 2005-07-29 2008-09-03 陶志伦 Method for catching fly maggot, and maggot catcher
US9241982B2 (en) 2009-01-16 2016-01-26 Sanaria Inc. Purified plasmodium and vaccine compositions
US10272146B2 (en) 2009-01-16 2019-04-30 Sanaria Inc. Purified plasmodium and vaccine compositions
US9616115B2 (en) 2009-01-16 2017-04-11 Sanaria Inc. Purified plasmodium and vaccine compositions
US8821896B2 (en) 2009-01-16 2014-09-02 Sanaria Inc. Purified Plasmodium and vaccine composition
US8992944B2 (en) 2009-01-16 2015-03-31 Sanaria Inc. Purified Plasmodium and vaccine composition
CN101984850A (en) * 2010-12-03 2011-03-16 广东省昆虫研究所 Housefly adult feed
CN102550487A (en) * 2011-01-21 2012-07-11 中国人民解放军第四五四医院 Production method of aseptic maggots
US9642909B2 (en) 2011-05-11 2017-05-09 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
US9278125B2 (en) 2011-05-11 2016-03-08 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
CN102550495B (en) * 2012-03-05 2013-11-20 联南强(漳州)生态农业有限公司 Method for culturing and domesticating fly species
CN102550495A (en) * 2012-03-05 2012-07-11 厦门联南强生物环保科技有限公司 Method for culturing and domesticating fly species
CN103798191A (en) * 2012-11-12 2014-05-21 安徽师范大学 Method for preventing and controlling waxberry orchard fruit flies by using housefly pupas to replace fruit fly pupas for breeding parasitic wasps
US9764016B2 (en) 2013-01-25 2017-09-19 Sanaria Inc. Genetic attenuation of Plasmodium by B9 gene disruption
US9931389B2 (en) 2013-01-25 2018-04-03 Sanaria Inc. Genetic attenuation of plasmodium by B9 gene disruption
CN104472441A (en) * 2014-12-04 2015-04-01 桐乡市恒易生物科技有限公司 Feeding method of hylemyia platura meigen
CN108770789A (en) * 2018-02-11 2018-11-09 宁波七禾加百列农业科技有限公司 Fly pieces of an egg hatch material and its hatching method
CN108935343A (en) * 2018-08-10 2018-12-07 陈希月 A kind of method and maggot bud albumen powder of artificial feeding maggot bud

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