CN117737204A - Biosensor and detection device - Google Patents
Biosensor and detection device Download PDFInfo
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- CN117737204A CN117737204A CN202311764155.8A CN202311764155A CN117737204A CN 117737204 A CN117737204 A CN 117737204A CN 202311764155 A CN202311764155 A CN 202311764155A CN 117737204 A CN117737204 A CN 117737204A
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- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 65
- 230000007246 mechanism Effects 0.000 claims abstract description 65
- 238000012360 testing method Methods 0.000 claims abstract description 41
- 108091093037 Peptide nucleic acid Proteins 0.000 claims abstract description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 12
- 239000002853 nucleic acid probe Substances 0.000 claims abstract description 9
- 229960002685 biotin Drugs 0.000 claims abstract description 6
- 235000020958 biotin Nutrition 0.000 claims abstract description 6
- 239000011616 biotin Substances 0.000 claims abstract description 6
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- 230000002572 peristaltic effect Effects 0.000 claims description 15
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
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- 230000001105 regulatory effect Effects 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 6
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- 210000003437 trachea Anatomy 0.000 claims 1
- 108700011259 MicroRNAs Proteins 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 238000003380 quartz crystal microbalance Methods 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a biosensor and a detection device, which belong to the technical field of sensors, wherein a peptide nucleic acid probe is arranged on a QCM (QCM) biosensor chip of the biosensor, and biotin is arranged on the peptide nucleic acid probe. The detection device comprises a network analyzer, a control terminal, a controller, a temperature control mechanism, an array type solution tank mechanism and a liquid supply mechanism, wherein the array type solution tank mechanism and the liquid supply mechanism are arranged in the temperature control mechanism, and a plurality of biological sensors are arranged in the array type solution tank mechanism. Adopt a biosensor and detection device of above-mentioned structure, improve microRNA's detection's degree of accuracy to carry out the multitime through array solution pond mechanism simultaneously and detect, make the testing process be in the constant temperature state through control by temperature change mechanism, through the accurate liquid feed volume of control of feed mechanism and wash.
Description
Technical Field
The invention relates to the technical field of sensors, in particular to a biosensor and a detection device.
Background
Quartz Crystal Microbalance (QCM) is a precise mass measuring instrument capable of being accurate to nanogram level, and is widely applied to analysis of various substance components in gas phase and liquid phase in the fields of biology, chemistry, environment, aerospace and the like. The detection target of the QCM in the prior art can be biomolecules, such as nucleic acid molecules, proteins, polysaccharides and the like, and the concentration of the biotin can be obtained by measuring the resonance frequency offset of the QCM, and a method of fluorescent labeling and enzyme labeling is not needed. In the prior art, DNA probes are adopted to detect microRNA, so that the stability is poor and the detection precision is low. Meanwhile, the existing detection device also has a plurality of problems, for example, the patent with the existing patent number of CN201720399925.7 discloses a portable QCM testing device easy to disassemble and wash, the aim of cleaning is achieved through a structure convenient to disassemble, but the prior art also has the following problems:
(1) The prior art device can only detect a sample once, and when the detection needs to be carried out for a plurality of times, the experiment needs to be repeated, and the time consumption is long.
(2) The added sample liquid type is not accurate enough, and the detection process needs constant temperature condition simultaneously, and temperature variation influences the stability of biomolecules, influences the detection accuracy.
(3) The disassembly and cleaning are needed, the nitrogen is used for drying in the practical process, the operation is complex, and the pollution is easy to happen.
Disclosure of Invention
The invention aims to provide a biological molecule detection device based on a QCM biological sensor, which solves the problems.
In order to achieve the above object, the present invention provides a biosensor, which comprises a QCM bio-sensor chip, wherein a peptide nucleic acid probe is provided on the QCM bio-sensor chip, and biotin for amplifying signals is provided on the peptide nucleic acid probe.
The detection device based on the biosensor comprises a network analyzer, a control terminal and a controller, wherein the controller and the network analyzer are electrically connected with the control terminal, the detection device comprises a temperature control mechanism, an array type solution tank mechanism and a liquid supply mechanism, the array type solution tank mechanism and the liquid supply mechanism are arranged in the temperature control mechanism, a plurality of biosensors are arranged in the array type solution tank mechanism, the biosensors are electrically connected with the network analyzer, and the temperature control mechanism and the liquid supply mechanism are electrically connected with the controller.
Preferably, the temperature control mechanism comprises a constant temperature seat and a plurality of temperature sensors inserted on the array type solution tank mechanism, the bottom and the side wall of the constant temperature seat are embedded with fixed constant temperature sheets in parallel, and the temperature sensors and the fixed constant temperature sheets are electrically connected with the controller.
Preferably, the constant temperature seat is dustpan-shaped, and a chute is arranged at the bottom of the constant temperature seat.
Preferably, the array type solution tank mechanism comprises a bottom plate and a top plate;
the top plate is provided with a plurality of test grooves arranged in an array, one side of the test groove is provided with a chip groove, a sealing rubber ring is arranged in the chip groove, the test groove is provided with a sensor jack, a liquid inlet hole and a liquid outlet hole, the top plate at two sides of the test groove is internally provided with a temperature regulating groove, the temperature regulating groove is internally provided with a plug-in type constant temperature sheet, a biosensor is arranged in the chip groove, and the temperature sensor is inserted in the sensor jack;
the bottom plate bottom sets up the slider, and the slider setting is in the spout, and threaded connection hole has all been seted up at roof and bottom plate both ends, and roof and bottom plate pass through bolt fixed connection.
Preferably, the fixed type constant temperature sheet and the plug type constant temperature sheet comprise a plurality of semiconductor heating sheets and semiconductor refrigerating sheets which are alternately arranged in parallel.
Preferably, the liquid supply mechanism comprises a peristaltic pump and a sample placement plate;
the bottom of the sample placing plate is provided with a sliding block, the sample placing plate is provided with a plurality of placing holes, and a temperature guide plate is fixed in each placing hole;
the sample container placed on the temperature guide plate is connected with a peristaltic pump through a liquid taking conduit, the liquid taking conduit is provided with a liquid taking valve, the peristaltic pump is connected with a liquid inlet hole through a liquid inlet conduit, the liquid inlet conduit is provided with a liquid inlet valve, the liquid outlet hole is connected with a liquid discharge pipe, and the liquid discharge pipe is provided with a liquid discharge valve;
the peristaltic pump is electrically connected with the controller through the motor driver, and the liquid taking valve, the liquid inlet valve and the liquid discharging valve are all electrically connected with the controller.
Preferably, the QCM biological sensing chip adopts an AT-cutQCM chip of 8 MHz.
Preferably, the automatic air blowing device further comprises a blowing mechanism, the blowing mechanism comprises a blowing fan, the blowing fan is connected with a nitrogen gas tank, an air outlet of the blowing fan is connected with the test groove through an air pipe, and the blowing fan is electrically connected with the controller.
Accordingly, the biosensor and the detecting device according to the present invention having the above-described structure comprise the following components
The beneficial effects are that:
(1) The peptide nucleic acid probe is adopted, and biotin for amplifying signals is arranged on the peptide nucleic acid probe, so that the detection precision of microRNA is improved.
(2) The array type solution tank mechanism is adopted to realize the detection of the same sample for multiple times or simultaneously detect multiple samples, so that the time consumed by repeated experiments is greatly reduced.
(3) The temperature control mechanism enables the detection process to be in a constant temperature state, and detection accuracy is guaranteed.
(4) The liquid feeding amount is accurately controlled and the liquid is flushed through the liquid feeding mechanism, and meanwhile, the drying operation is performed through the drying mechanism, so that the natural drying time is avoided to be too long, the operation is simple, and the pollution is not easy to cause.
The technical scheme of the invention is further described in detail through the drawings and the embodiments.
Drawings
FIG. 1 is a schematic diagram of a detecting device with a biosensor without a blow-drying mechanism according to the present invention;
FIG. 2 is a side view of the thermostatic seat of the present invention;
fig. 3 is a layout of a semiconductor cooling sheet and a semiconductor heating sheet;
FIG. 4 is a schematic diagram showing the structure of a solution tank of a test tank;
FIG. 5 is a schematic view of a detection device with a blow-drying mechanism and a biosensor;
FIG. 6 is a graph of resonance frequency data at different concentrations of microRNA.
Reference numerals
1. A network analyzer; 2. a control terminal; 3. a controller; 4. a temperature control mechanism; 41. a constant temperature seat; 411. a chute; 42. a temperature sensor; 43. a fixed thermostatic sheet; 44. plug-in type constant temperature sheet; 45. a semiconductor heating sheet; 46. a semiconductor refrigeration sheet; 5. an array type solution tank mechanism; 51. a bottom plate; 52. a top plate; 521. a test slot; 522. a chip slot; 523. sealing rubber rings; 524. a liquid inlet hole; 525. a liquid outlet hole; 526. a temperature regulating tank; 6. a liquid supply mechanism; 61. a peristaltic pump; 62. a sample placement plate; 63. a temperature guide plate; 64. a liquid taking valve; 65. a liquid inlet valve; 66. a liquid discharge valve; 7. a biosensor; 8. a blow-drying mechanism; 81. blowing the blower; 82. and (5) nitrogen filling.
Detailed Description
Examples
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "upper", "lower", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Some embodiments of the present invention are described in detail below with reference to the accompanying drawings.
Example 1
As shown in fig. 1, a detection device with a biosensor includes a network analyzer 1, a control terminal 2, a controller 3, a temperature control mechanism 4, an array type solution tank mechanism 5, and a liquid supply mechanism 6.
The model of the network analyzer 1 is KEYSIGHTE5061B, the sweep frequency range of the network analyzer 1 is set to be 7.92M-7.99 MHz, the number of sweep frequency points is set to be the maximum number 1601, the control terminal 2 is a personal computer, and the controller 3 is a single-chip microcomputer controller. The controller 3 and the network analyzer 1 are electrically connected with a personal computer, so that data acquisition, processing and display are realized.
The biosensor 7 comprises a QCM biosensor chip on which Peptide Nucleic Acid (PNA) probes are arranged, the PNA (peptide nucleic acid) has the ability to specifically bind to RNA of a specific sequence, following the base complementary pairing principle, PNA is uncharged and binds to negatively charged target RNA more easily than negatively charged DNA, RNA probes. PNA is a backbone of peptide chains that is much more stable than RNA and DNA. In order to reduce the detection limit and improve the detection sensitivity, biotin for amplifying a signal is provided on the peptide nucleic acid probe (nanogold is used in this example).
In order to ensure that the detected biomolecules are in a constant temperature environment, an array type solution tank mechanism 5 and a liquid supply mechanism 6 are placed by a temperature control mechanism 4, as shown in fig. 2, the temperature control mechanism comprises a constant temperature seat 41 and a plurality of temperature sensors 42 inserted on the array type solution tank mechanism 5, the constant temperature seat 41 is in a dustpan shape, and a chute 411 is formed in the bottom of the constant temperature seat 41. The bottom and the side wall of the constant temperature seat 41 are embedded with fixed constant temperature sheets 43 in parallel, and the temperature sensor 42 and the fixed constant temperature sheets 43 are electrically connected with the controller 3.
In order to perform multiple tests on the same sample or to perform simultaneous tests on multiple samples, the present embodiment uses the array-type solution cell mechanism 5 for the tests. The array solution tank mechanism 5 comprises a bottom plate 51 and a top plate 52, the top plate 52 of the embodiment is provided with 3 parallel test grooves 521, as shown in fig. 4, one side of the test groove 521 is provided with a chip groove 522, the biosensor 7 adopts an 8MHz AT-cutQCM chip, the resonance frequency is between 7.92M and 7.99MHz and is placed in the chip groove 522, and the chip groove 522 is internally provided with a sealing rubber ring 523 to avoid chip damage. The test tank 521 is provided with a sensor jack, a liquid inlet 524 and a liquid outlet 525, the top plate 52 at two sides of the test tank 521 is provided with a temperature regulating tank 526, the temperature regulating tank 526 is internally provided with a plug-in type constant temperature sheet 44, the biosensor 7 is arranged in the chip tank 522, and the temperature sensor 42 is inserted in the sensor jack. The bottom plate 51 is provided with a sliding block at the bottom, and the sliding block is arranged in the sliding groove 41, so that the array type solution tank mechanism 5 is conveniently adjusted to the position of the constant temperature seat 41, and is convenient to maintain or install. Threaded connection holes are formed in two ends of the top plate 52 and the bottom plate 51, the top plate 52 and the bottom plate 51 are fixedly connected through bolts, a sandwich structure is adopted, the biosensor 7 is convenient to replace, and the top plate 52 and the bottom plate 51 are convenient to clean. As shown in fig. 3, the fixed thermostatic sheet 43 and the plug-in thermostatic sheet 44 each include a plurality of semiconductor heating sheets 45 and semiconductor cooling sheets 46 alternately arranged in parallel, the semiconductor heating sheets 45 and the semiconductor cooling sheets 46 are electrically connected with the controller 3, the controller 3 adopts PID control principle to adjust the temperature, and the controller 3 controls the start, stop and working power of the semiconductor heating sheets 45 and the semiconductor cooling sheets 46 at corresponding positions according to the data of the temperature sensor 42. A number of biosensors 7 are electrically connected to the network analyzer 1 for collecting frequency data.
In order to provide a stable flow rate and a fixed volume of test liquid or rinse liquid to each test tank 521, the liquid supply mechanism 6 of the present embodiment includes a peristaltic pump 61 and a sample placement plate 62, a slider is disposed at the bottom of the sample placement plate 62, a plurality of placement holes are formed in the sample placement plate 62, and a thermal conductive plate 63 (made of copper) is fixed in the placement holes. The sample container placed on the temperature-guiding plate 63 is connected with the peristaltic pump 61 through a liquid-taking conduit provided with a liquid-taking valve 64, the peristaltic pump 61 is connected with a liquid-feeding hole 524 through a liquid-feeding conduit provided with a liquid-feeding valve 65, the liquid-discharging hole 525 is connected with a liquid-discharging pipe provided with a liquid-discharging valve 66, the peristaltic pump 61 is electrically connected with the controller 3 through a motor driver, and the liquid-taking valve 64, the liquid-feeding valve 65 and the liquid-discharging valve 66 are all electrically connected with the controller 3.
As shown in fig. 5, the air blowing mechanism 8 is also provided, the air blowing mechanism 8 comprises a blowing fan, the blowing fan is connected with a nitrogen gas tank 82, an air outlet of the blowing fan is connected with the test groove 521 through an air pipe, the blowing fan is electrically connected with the controller 3, and nitrogen gas blowing after flushing is realized.
The specific experimental process is as follows:
the test was performed by using bovine serum albumin solids produced by BIOSHARP in a 0.1% BSA solution with water.
The test process comprises the following steps:
the preparation of the drug is as follows:
1. sodium dihydrogen phosphate, chemically produced in the morning, was formulated as 1MPBS buffer and ph=7 was adjusted with NaOH.
2. Single stranded RNA produced by the organism of the species ebonite, 5nmol total.
The sequence is as follows: 5-auaagacgagcaaaaagcuugu-3.
MicroRNA solutions of 0.05. Mu. Mol/L, 0.1. Mu. Mol/L, 0.2. Mu. Mol/L, 0.5. Mu. Mol/L, 1. Mu. Mol/L, 5. Mu. Mol/L, 10. Mu. Mol/L, 20. Mu. Mol/L, 50. Mu. Mol/L, 100. Mu. Mol/L were prepared with 1 MPBS.
4. Ethanol and sterile water.
First, rinsing and blow-drying are performed.
The three test slots 521 were rinsed with ethanol and sterile water sequentially by the liquid supply mechanism 6 for a rinsing time of 10 minutes. After the washing is finished, the test groove 521 is dried by the drying mechanism 8.
Then, the temperature control mechanism 4, the controller 3 and the control terminal 2 are activated, the sample container is set in the plate to ensure the temperature of the test sample to be constant, and the sample container is connected to the peristaltic pump 61 through the liquid-taking tube.
Finally, a peristaltic pump 61, a liquid taking valve 64 and a liquid inlet valve 65 are started to respectively provide 20 microliters of 0.05 mu mol/L,0.1 mu mol/L and 0.2 mu mol/L of microRNA solution for three test tanks 521, the data of the output resonance frequency of three concentration RNAs are obtained by continuously measuring for 20 minutes through a network analyzer 1, a liquid discharge valve is started to discharge liquid after the test is finished, a liquid supply mechanism is used for flushing the test tanks by adopting 1MPBS buffer solution for 2 minutes, the next group of detection of microRNA solutions with different concentrations is carried out, the steps are repeated until all the microRNA solutions with different concentrations are detected, and finally the test tanks are flushed through ethanol and sterile water.
The experimental results are shown in FIG. 6.
At RNA concentrations of 0.05 to 0.2. Mu. Mol/L, the resonance frequency decreased with increasing RNA concentration, indicating that the biosensor in this example was relatively accurate in detection at RNA concentrations of less than 0.2. Mu. Mol/L. The length of the line segment at each point value represents the variance size. After that, when the RNA concentration increases again, the resonance frequency does not remain decreasing any more, and when the RNA concentration exceeds 0.2. Mu. Mol/L, the frequency does not decrease any more, for the following reasons:
1. since the binding of the RNA to be detected and the PNA probe is saturated, further increase of the concentration of RNA does not increase the mass of nucleic acid attached to the electrode surface.
2. The measurement of different concentrations of RNA is not simultaneous. Higher concentration RNA samples are degraded very much after the measurement time. The number of test slots can be increased to reduce errors due to degradation.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention and not for limiting it, and although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that: the technical scheme of the invention can be modified or replaced by the same, and the modified technical scheme cannot deviate from the spirit and scope of the technical scheme of the invention.
Claims (9)
1. The biosensor comprises a QCM biological sensing chip, and is characterized in that: the QCM biological sensing chip is provided with a peptide nucleic acid probe, and the peptide nucleic acid probe is provided with biotin for amplifying signals.
2. The biosensor-based detection device according to claim 1, comprising a network analyzer, a control terminal and a controller, wherein the controller and the network analyzer are electrically connected with the control terminal, and the biosensor-based detection device is characterized in that: including control by temperature change mechanism, array solution pond mechanism and feed liquid mechanism all set up in control by temperature change mechanism, are provided with a plurality of biosensors in the array solution pond mechanism, and a plurality of biosensors are connected with the network analyzer electricity, and control by temperature change mechanism and feed liquid mechanism all are connected with the controller electricity.
3. A test device according to claim 2, wherein: the temperature control mechanism comprises a constant temperature seat and a plurality of temperature sensors inserted on the array type solution tank mechanism, fixed constant temperature sheets are embedded in the bottom and the side wall of the constant temperature seat in parallel, and the temperature sensors and the fixed constant temperature sheets are electrically connected with the controller.
4. A test device according to claim 3, wherein: the constant temperature seat is dustpan-shaped, and a chute is arranged at the bottom of the constant temperature seat.
5. A test device according to claim 4, wherein: the array type solution tank mechanism comprises a bottom plate and a top plate;
the top plate is provided with a plurality of test grooves arranged in an array, one side of the test groove is provided with a chip groove, a sealing rubber ring is arranged in the chip groove, the test groove is provided with a sensor jack, a liquid inlet hole and a liquid outlet hole, the top plate at two sides of the test groove is internally provided with a temperature regulating groove, the temperature regulating groove is internally provided with a plug-in type constant temperature sheet, a biosensor is arranged in the chip groove, and the temperature sensor is inserted in the sensor jack;
the bottom plate bottom sets up the slider, and the slider setting is in the spout, and threaded connection hole has all been seted up at roof and bottom plate both ends, and roof and bottom plate pass through bolt fixed connection.
6. A test device according to claim 5, wherein: the fixed type constant temperature sheet and the plug type constant temperature sheet comprise a plurality of semiconductor heating sheets and semiconductor refrigerating sheets which are alternately arranged in parallel.
7. A test device according to claim 6, wherein: the liquid supply mechanism comprises a peristaltic pump and a sample placement plate;
the bottom of the sample placing plate is provided with a sliding block, the sample placing plate is provided with a plurality of placing holes, and a temperature guide plate is fixed in each placing hole;
the sample container placed on the temperature guide plate is connected with a peristaltic pump through a liquid taking conduit, the liquid taking conduit is provided with a liquid taking valve, the peristaltic pump is connected with a liquid inlet hole through a liquid inlet conduit, the liquid inlet conduit is provided with a liquid inlet valve, the liquid outlet hole is connected with a liquid discharge pipe, and the liquid discharge pipe is provided with a liquid discharge valve;
the peristaltic pump is electrically connected with the controller through the motor driver, and the liquid taking valve, the liquid inlet valve and the liquid discharging valve are all electrically connected with the controller.
8. A test device according to claim 7, wherein: the QCM biological sensing chip adopts an AT-cutQCM chip of 8 MHz.
9. A test device according to claim 8, wherein: still including drying the mechanism, it includes and weathers the fan to weather the mechanism, weathers the fan and is connected with the nitrogen gas and irritates, weathers the air outlet of fan and is connected with the test tank through the trachea, weathers the fan and is connected with the controller electricity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311764155.8A CN117737204A (en) | 2023-12-20 | 2023-12-20 | Biosensor and detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311764155.8A CN117737204A (en) | 2023-12-20 | 2023-12-20 | Biosensor and detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117737204A true CN117737204A (en) | 2024-03-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311764155.8A Pending CN117737204A (en) | 2023-12-20 | 2023-12-20 | Biosensor and detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117737204A (en) |
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2023
- 2023-12-20 CN CN202311764155.8A patent/CN117737204A/en active Pending
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