CN117539043A - Portable microscope device for separating biological samples or non-biological particles and application - Google Patents
Portable microscope device for separating biological samples or non-biological particles and application Download PDFInfo
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- G—PHYSICS
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K1/00—Arrangements for handling particles or ionising radiation, e.g. focusing or moderating
- G21K1/006—Manipulation of neutral particles by using radiation pressure, e.g. optical levitation
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Abstract
The invention discloses a portable microscope device, which comprises an optical tweezers control system and a single cell sorting system, wherein the optical tweezers control system comprises a microscope, an imaging device and a laser generating device, wherein the imaging device and the laser generating device are connected with the microscope, and an imaging light path of the microscope and a capturing light path of the laser generating device share a lens; the single-cell sorting system comprises a pressure control device, a micromanipulation device and a micro-nano needle. The invention also discloses a method for separating biological samples or non-biological particles by using the portable microscope device. The portable microscope device has the characteristics of portability and detachability, can be used for performing in-situ experiments in the field, and has great application prospects in biological research in the fields of biomedicine, environmental science and earth science.
Description
Technical Field
The present invention relates to a portable microscope device for separating biological samples or non-biological particles, and a separation method and application.
Background
The microscopy technology is a key for opening the microscopic world by human beings, and is a necessary tool for developing scientific researches. The laser microscope combines the optical microscope with the modern laser technology to carry out high-sensitivity detection and high-resolution imaging on various biological cell structures and specific biological molecules, and has wide application in the field of life medicine. In recent years, with the scientific development and the crossing demands of disciplines, development of a new generation of laser microscope is urgently needed, and rapid and ultra-high resolution imaging and nondestructive and efficient multi-parameter analysis are carried out on various samples including biological cells, cosmic dust, dust haze particles and nano materials under the micro-nano scale.
Microorganisms are the oldest, most widely occurring, most abundant and diverse life forms on earth, the first stage engine driving the world's bio-geochemical cycle. Despite the wide variety of microorganisms, only 1% of the strains are currently cultivated in pure form due to various restriction factors, which greatly hinders the progress of microbial research and the comprehensive development and utilization of a huge treasury of human beings on microbial resources. Along with the development of single-cell sequencing technology, electron microscopy, biological materials and other technologies, scientists propose a single-cell sorting method, and after single cells are separated from a complex sample, the single cells are subjected to expansion culture and subsequent experiments; even research methods for bacterial environmental samples of independent pure culture systems have been established. In summary, single cell isolation techniques offer more possibilities for investigation of uncultured microorganisms. At present, common single-cell separation technologies comprise a micromanipulation technology, fluorescence activation separation, microfluidic separation and the like, but most of the technologies are single use, so that single cells are subjected to biological identification or information characterization, the types and the number of captured samples are limited, and the information quantity of the acquired samples is limited to different degrees for subsequent experiments.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides a portable microscope device, an optical tweezers system and a single-cell sorting system are carried on the basis of an optical microscope, and a micro-nano scale living cell, a biological macromolecule and a single-particle sample are accurately manipulated, captured and separated through the single-cell sorting system, so that a trace sample can be used for a series of experiments outside a system such as a subsequent scanning electron microscope, a transmission electron microscope, a molecular biology experiment, a Raman spectrum analysis and the like to acquire more sample information quantity; in addition, the most critical is that the equipment has the characteristics of portability and detachability, can be used for performing in-situ experiments in the field, provides more possibility for research and exploration of microorganisms, and is expected to generate huge application prospects in the fields of biomedicine, environmental science and earth science. The device can be further combined with a magnetic control system, and can capture magnetically responsive microorganisms, capture special groups such as magnetic sensitivity, too high movement speed and the like by photo-magnetic combination, and acquire microparticles, biological macromolecules and the like.
The first aspect of the invention provides a portable microscope device, which comprises an optical tweezers control system and a single-cell sorting system, wherein the optical tweezers control system comprises a microscope, an imaging device and a laser generating device, wherein the imaging device is connected with the microscope, and an imaging light path of the microscope and a capturing light path of the laser generating device share a lens; the single-cell sorting system comprises a pressure control device, a micromanipulation device and a micro-nano needle.
In some embodiments, the microscope is selected from an inverted microscope. Preferably, the microscope may be selected from an optical inverted microscope, a laser inverted microscope or a super-resolution inverted microscope.
In some embodiments, the imaging device comprises a camera and a companion image visualization device. The imaging device can observe the sample in real time, can accurately and rapidly determine the position of the sample, and is more beneficial to the separation and collection of the sample by micromanipulation.
In some embodiments, the laser generating device is selected from an infrared laser light source. Preferably, the wavelength of the infrared laser light source is 750nm-1200nm, preferably 1000-1100nm. Different biological tissues have different light absorption degrees in different wave bands, and the wave band can be selected to avoid the absorption bands of the biological tissues and surrounding media, so that the thermal damage of highly focused laser to a sample is reduced.
In some embodiments, the imaging optical path of the microscope shares a lens with the capture optical path of the laser generating device.
In some embodiments, the pressure control device comprises a pneumatic injector, a connecting tube, and a metal cylinder, one end of the connecting tube is provided with a connector connected with the pneumatic injector, and the other end of the connecting tube is connected with the tail end of the metal cylinder.
In some embodiments, the pneumatic injector is provided with an omnibearing rotary knob, pneumatic operation is realized by controlling the rotary knob, so that maintenance problems such as oiling, refilling or bubble removal are avoided, stable trace air intake can be ensured, and fluctuation is small.
In some embodiments, the pneumatic injector can be rotated by 2-10mm by rotating the knob in all directions, enabling control of 50-500 μl of liquid. The liquid volume is controlled by the length of the connecting pipe and the moving distance of the omnibearing knob.
In some embodiments, the micromanipulation device includes a micromanipulation hand for controlling the micro-nano needle and a holder disposed at an upper portion of the micromanipulation hand, the holder for holding the metallic syringe.
In some embodiments, the micro-nanoneedle is mounted to the metallic syringe tip.
In some embodiments, the micromanipulator is provided with a movable platform capable of moving the micro-nanoneedle. The micro-manipulator can adjust the micro-nano needle in three dimensions of horizontal, up-down and front-back so that the micro-nano needle can move in the whole sample liquid drop. Moreover, the micro manipulator can realize the movement of the micro-nano needle in the micro-nano scale through the coarse focusing spiral and the fine focusing spiral.
In some embodiments, the micro-nanoneedle is a capillary needle. Preferably, the aperture of the micro-nano needle is 1-20 μm, preferably 1-15 μm. Preferably, the length of the micro-nano needle is 2-20cm, preferably 5-10cm. Within the above range, the aperture and length of the capillary needle can be controlled to be adjusted according to the sample size, and the selection of an appropriate capillary aperture size is advantageous for retaining the separated sample within the capillary.
In some embodiments, the connection tube is a polyethylene tube or a silicone hose, preferably a polyethylene tube.
In some embodiments, preferably, the connecting tube has an inner diameter of 0.5-1.5mm, preferably 0.8-1.2mm,
preferably, the outer diameter of the connecting tube is 0.1-0.8cm, preferably 0.1-0.5cm;
preferably, the length of the connecting pipe is 20-200cm.
In some embodiments, the portable microscope device further comprises a magnetic control system comprising at least one set of parallel coils and a magnetic field controller. The magnetic control system can provide various magnetic field forms and can separate samples with magnetic response.
In some embodiments, the magnetron system includes a first set of parallel coils and a second set of parallel coils perpendicular to the first set of parallel coils to form a cube structure.
In some embodiments, the parallel coils of the magnetic control system are fixed to a stage of a microscope of the optical tweezers system. Preferably, the parallel coils are sized to be securable between a stage of the microscope and an objective lens.
In some embodiments, the magnetic field ranges from 0 to 10G.
In some embodiments, the magnetic field comprises at least one of a uniform magnetic field, a rotating magnetic field, a constant magnetic field, an alternating magnetic field (square wave, sinusoidal, triangular wave), a universal magnetic field, a swinging magnetic field, a pulsed magnetic field, a gradient magnetic field, and a composite magnetic field of a plurality of magnetic field combinations.
In some embodiments, the uniform magnetic field is a uniform magnetic field in any direction in a horizontal plane.
In some embodiments, the rotating magnetic field is a rotating magnetic field with a variable frequency in the horizontal plane, preferably with a maximum frequency of 0-20Hz.
In some embodiments, the portable microscope device further comprises a support for integrally securing the optical tweezers system and/or the single cell sorting system.
In some embodiments, the portable microscope device further comprises a raman spectrometer for raman analysis.
A second aspect of the present invention provides a method of separating biological samples or non-biological particles using a portable microscopy device according to the first aspect, the method comprising the steps of: (1) capturing a target sample in an optical tweezers system; (2) isolating the target sample in a single cell sorting system.
In some embodiments, step (1) comprises the steps of:
(1-1) placing a sample to be separated on a stage of a microscope, determining a target sample by the microscope and an imaging device, and moving it to a field of view of the microscope;
(1-2) turning on a laser generating device, and fixing the target sample by using an optical trap.
In some embodiments, step (2) comprises the steps of:
(2-1) moving a micro-nanoneedle to the target sample by a micromanipulator;
(2-2) sucking the target sample into the micro-nano needle by the pressure control device.
Preferably, in the step (2-2), the pressure in the micro-nano needle is reduced by the pressure control device, so that the target sample is sucked into the micro-nano needle. Preferably, the volume of liquid aspirated is carefully controlled by a pneumatic microsyringe.
In some embodiments, the method further comprises magnetically separating the sample to be separated in a magnetic control system prior to step (1).
In some embodiments, the magnetic separation comprises: and setting a magnetic field through a magnetic control system, and moving the sample to a target position by utilizing the magnetic field.
In some embodiments, the biological sample comprises at least one of a biological cell, a biological macromolecule, a microparticle. Preferably, the biological cells comprise protozoa and/or bacteria, preferably at least one living cell comprising ciliates, cyanobacteria, magnetotactic bacteria. In some embodiments, the biological sample is a protozoa and/or bacteria that move at a relatively high rate.
In some embodiments, the non-biological particles comprise micro-nanoparticles in an environmental sample.
In some embodiments, the method further comprises subjecting the sample obtained by the isolating to an experimental analysis. Preferably, the experimental analysis comprises at least one of transmission electron microscopy, scanning electron microscopy and/or DNA sequencing.
A third aspect of the invention provides the use of a portable microscope device as described in the first aspect or a method as described in the second aspect in biomedical, environmental and earth science fields.
In some embodiments, for field in situ experimental studies.
In the portable microscope device, a cell sample can be fixed through an optical trap formed by an optical path of an optical tweezers control system, and then captured by a single cell sorting system. The portable Shan Guangnie device is adopted in the optical tweezers system, and based on the design that a laser capturing optical path and an imaging optical path share a lens and an optical path space, the device is miniaturized, and the device is convenient to install and carry. The single-cell sorting system can control the micro-nano needle to separate single cells, biological macromolecules, single particles and the like under a microscope based on a micro manipulator, and can accurately control the absorption and release of a target object. The device is miniaturized and portable and detachable. The optical tweezers can fix particles such as floating cells, macromolecules and the like in the solution. The combination of the optical tweezers technology and the microscopic technology can quantitatively analyze the interaction between subcellular structures and molecules, and provide unprecedented imaging precision and precise control for the research of cell biology. Most importantly, the method can be used for performing non-invasive control on biological cells, and has important biological effects. Furthermore, the magnetic control system can provide various magnetic field forms, and feedback control is applied to a sub-magnetic space system combined with a microscope, so that automatic parameter configuration of the sub-magnetic space control system is realized, and meanwhile, a specified magnetic field can be loaded in any specified direction, so that accurate manipulation and measurement can be performed on a sample.
The portable microscope device and the separation method can be applied to biological samples which are difficult to capture due to weak mobility, fast mobility or no mobility (such as ciliates and blue algae) and biological samples which are responsive to a magnetic field but have small sample quantity, and the biological samples are captured and collected by using an optical tweezers control system and a single cell sorting system and the research of subsequent related experiments. Due to the characteristics of miniaturization and portability, the environment sample can be brought to the field, target samples, particularly biological samples, in the environment sample can be captured and collected under the in-situ condition, the required experiment can be carried out later, and the state of the environment sample under the in-situ condition can be maintained more.
Drawings
Fig. 1 shows a schematic configuration of a portable microscope device of the present invention.
Fig. 2 shows a schematic structural diagram of the optical tweezers system of the present invention, wherein 1 is a microscope, 2 is an imaging device, and 3 is a laser generating device.
FIG. 3 shows a schematic diagram of the structure of the single cell sorting system of the present invention.
Fig. 4 shows a schematic structural diagram of a pressure control device in a single-cell sorting system according to the present invention, wherein 4 is a pneumatic injector, 5 is a connector, 6 is a connecting tube, 7 is a metallic needle cylinder, and 8 is a micro-nano needle.
Fig. 5 shows a schematic structural view of a micromanipulation device in a single cell sorting system according to the present invention, wherein 9 is a micromanipulator and 10 is a holder.
Fig. 6 shows a schematic structural diagram of the magnetic control system of the present invention, wherein 11 is a magnetic field controller and 12 is a parallel coil.
Fig. 7 illustrates an optical trap control capture target sample formed using a portable microscope device of the present invention.
Fig. 8 shows the collection of a target sample using the single cell manipulation system of the portable microscope device of the present invention.
Fig. 9 shows a TEM photograph of a target sample collected using the portable microscope device of the present invention.
Detailed Description
The present invention will be further described in detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. The specific embodiments described herein are for purposes of illustration only and are not to be construed as limiting the invention in any way. In addition, in the following description, descriptions of well-known structures and techniques are omitted so as not to unnecessarily obscure the concepts of the present disclosure. Such structures and techniques are also described in a number of publications.
The present invention will be described in further detail with reference to the accompanying drawings.
Referring to fig. 1, in some embodiments, a portable microscope device is provided that includes an optical tweezers control system, a single cell sorting system, and a magnetic control system, the optical tweezers control system and the single cell sorting system being integrally affixed to the same support. The magnetic control system is used for accurately manipulating the sample to be separated based on the action of the magnetic field, the optical tweezers control system is used for forming an optical trap based on the single optical tweezers device to fix the sample, and the single-cell sorting system is used for manipulating the micro-nano needle to separate the sample based on a micro-manipulator under a microscope.
The optical tweezers control system (see fig. 2) comprises an inverted microscope 1, an imaging device 2 and a laser generating device 3, wherein the imaging device 2 and the laser generating device 3 are connected with the inverted microscope, an imaging light path of the microscope 1 and a capturing light path of the laser generating device 3 share a lens, the imaging device 2 comprises a camera and a matched image visualizing device, and the infrared wavelength in the laser generating device 3 is 1064nm.
Wherein the single cell sorting system (see fig. 3) comprises a pressure control device, a micromanipulation device and a micro-nanoneedle 8. The pressure control device comprises a pneumatic injector 4, a connecting pipe 6 and a metal needle cylinder 7, wherein one end of the connecting pipe 6 is provided with a connector 5, the connector 5 is connected with the pneumatic injector 4, and the other end of the connecting pipe 6 is connected with the tail end of the metal needle cylinder 7 (see figure 4). The micromanipulation device comprises a micromanipulator 9 for controlling the micro-nanoneedles 8, the micromanipulator 9 being provided with a holder 10 at the upper part, to which holder 10 the metallic needle cylinder 7 is fixed in use (see fig. 5). A micro-nano needle 8 is mounted to the top end of the metal cylinder 7 (see fig. 4).
The magnetic control system (see fig. 6) comprises two sets of parallel coils 12 and a magnetic field controller 11, wherein the first set of parallel coils and the second set of parallel coils are mutually perpendicular to form a cube structure, and the parallel coils 12 are fixed on a stage of the microscope 1.
A method for separating biological samples or non-biological particles based on the portable microscope, comprising the following steps: (1) capturing a target sample in an optical tweezers system; (2) isolating the target sample in a single cell sorting system.
Wherein step (1) comprises: placing a sample to be separated on a stage of a microscope, determining a target sample through the microscope and an imaging device, and moving the target sample into the visual field of the microscope; and starting a laser generating device, and fixing the target sample by utilizing an optical trap.
Wherein, step (2) includes: moving the micro-nano needle to the target sample by a micro-manipulator; and reducing the pressure in the micro-nano needle through the pressure control device, and sucking the target sample into the micro-nano needle.
Wherein, if the sample to be separated has a magnetic response, the method further comprises magnetically separating the sample to be separated in a magnetic control system before the step (1). Wherein the magnetic separation comprises: and setting a magnetic field through a magnetic control system, and moving the sample to a target position by utilizing the magnetic field.
The above method further comprises subjecting the sample obtained by separation to experimental analysis including, but not limited to, transmission electron microscopy, scanning electron microscopy, DNA sequencing, etc.
The method for separating biological samples or non-biological particles according to the present invention is further described below by way of examples.
Example 1 capture of magnetotactic bacteria
The portable microscope device shown in fig. 1 is adopted to separate and collect the field collected samples of the magnetotactic bacteria and carry out subsequent analysis, and the specific steps are as follows:
1. observation of magnetotactic bacteria
(1) The slide was mounted on the microscope stage of an optical tweezers system, a drop of sediment sample was pipetted onto the slide using a plastic glue head dropper, about 20 μl of distilled water was dropped with a pipette at about 0.5-1cm from the right side of the sediment drop, and then a small amount of distilled water was dropped with a pipette to form a channel connecting the two drops.
(2) The magnetic control system is turned on, and the type, direction and size of the magnetic field are set (uniform magnetic field is selected in the embodiment, and the size is set to be 5G). The magnetotactic bacteria sample is then observed by a camera and a matched image visualization device to move along the magnetic field direction to the water edge of the distilled water drop.
2. Collection of magnetotactic bacteria
(1) Grinding a capillary needle with the aperture of 1-15 mu m and the length of 8-10cm by using a high-precision capillary drawing instrument (NARISHIGE PC-100) and a micro needle grinding instrument (NARISHIGEEG-401);
(2) Turning on a switch of a laser generating device of the optical tweezers system, and fixing a target magnetotactic bacteria sample by utilizing an optical trap, see fig. 7;
(3) And (3) placing the ground capillary needle at the position where the magnetotactic bacteria are positioned by the optical trap by using a single-cell sorting system, sucking the target strain by using the capillary needle through a microscopic manipulator (figure 8), then moving the sucked magnetotactic bacteria sample into the rightmost distilled water for cleaning, sucking the magnetotactic bacteria again by using the microscopic manipulator after cleaning, and transferring the magnetotactic bacteria sample into a centrifuge tube. The above procedure was repeated until enough samples of the desired magnetotactic bacteria were collected.
3. Subsequent experiments with magnetotactic bacteria
The obtained magnetotactic bacteria sample can be divided into three parts, and different related experiments are carried out.
(1) The first part is used for molecular biology experiments, and 16S rRNA gene sequence information of a magnetotactic bacterial sample is obtained.
(2) The second portion of the sample was used for Transmission Electron Microscopy (TEM) observation to obtain magnetotactic bacterial sample cells and internal magnetosome shape information (see fig. 9 for exemplary results).
(3) The third portion of the sample was used for Scanning Electron Microscope (SEM) observation to obtain morphology and taxonomic information of the magnetotactic bacterial sample at the single cell level.
The technical scheme of the invention is not limited to the specific embodiment, and all technical modifications made according to the technical scheme of the invention fall within the protection scope of the invention.
Claims (10)
1. A portable microscope device comprising:
the optical tweezers control system comprises a microscope, an imaging device and a laser generating device, wherein the imaging device and the laser generating device are connected with the microscope, and an imaging light path of the microscope and a capturing light path of the laser generating device share a lens; and
the single cell sorting system comprises a pressure control device, a micromanipulation device and a micro-nano needle.
2. The portable microscope device according to claim 1, wherein the microscope is selected from an inverted microscope, preferably from an optical inverted microscope, a laser inverted microscope or a super-resolution inverted microscope; and/or
The imaging device comprises a camera and a matched image visualization device; and/or
The laser generating device is selected from infrared laser light sources, preferably with a wavelength of 750nm-1200nm, preferably 1000-1100nm.
3. The portable microscope device according to claim 1 or 2, wherein the pressure control device comprises a pneumatic injector, a connection tube, and a metal cylinder, one end of the connection tube being provided with a connector connected to the pneumatic injector, the other end of the connection tube being connected to an end of the metal cylinder; and/or
The micro-operation device comprises a micro-operation hand for controlling the micro-nano needle and a fixer arranged at the upper part of the micro-operation hand, wherein the fixer is used for fixing the metal needle cylinder; and/or
The micro-nano needle is mounted on the top end of the metal needle cylinder;
preferably, the micro-nano needle is a capillary needle;
preferably, the aperture of the micro-nano needle is 1-20 μm, preferably 1-15 μm,
preferably, the length of the micro-nano needle is 2-20cm, preferably 5-10cm;
preferably, the connecting pipe is a polyethylene pipe or a silica gel hose;
preferably, the connecting tube has an inner diameter of 0.5-1.5mm, preferably 0.8-1.2mm,
preferably, the outer diameter of the connecting tube is 0.1-0.8cm, preferably 0.1-0.5cm;
preferably, the length of the connecting pipe is 20-200cm.
4. The portable microscope device according to any one of claims 1 to 3, further comprising a magnetic control system comprising at least one set of parallel coils and a magnetic field controller,
preferably, the magnetic control system comprises a first group of parallel coils and a second group of parallel coils which are mutually perpendicular to the first group of parallel coils to form a cube structure;
preferably, parallel lines of the magnetic control system are fixed on a stage of a microscope of the optical tweezers system;
preferably, the magnetic field is in the range of 0-10G;
preferably, the magnetic field comprises at least one of a uniform magnetic field, a rotating magnetic field, a constant magnetic field, an alternating magnetic field, a universal magnetic field, a swinging magnetic field, a pulsed magnetic field, a gradient magnetic field, and a composite magnetic field of a plurality of magnetic field combinations;
preferably, the uniform magnetic field is a uniform magnetic field in any direction in a horizontal plane;
preferably, the rotating magnetic field is a rotating magnetic field with variable frequency in a horizontal plane;
more preferably, the frequency is 0-20Hz.
5. The portable microscope device according to any one of claims 1 to 4, further comprising a support for integrally securing the optical tweezers system and/or the single cell sorting system; and/or
The portable microscope device further comprises a raman spectrometer for raman analysis.
6. A method of separating biological samples or non-biological particles using the portable microscope device of any one of claims 1-5, the method comprising the steps of:
(1) Capturing a target sample by the optical tweezers system;
(2) Isolating the target sample by the single cell sorting system;
preferably, step (1) comprises the steps of:
(1-1) placing a sample to be separated on a microscope stage, determining a target sample by a microscope and an imaging device, and moving it to a field of view of the microscope;
(1-2) turning on a laser generating device, and fixing the target sample by using an optical trap;
preferably, step (2) comprises the steps of:
(2-1) moving a micro-nanoneedle to the target sample by a micromanipulator;
(2-2) sucking the target sample into the micro-nano needle by the pressure control device.
7. The method of claim 6, further comprising magnetically separating the sample to be separated in a magnetic control system prior to step (1);
preferably, the magnetic separation comprises: and setting a magnetic field through a magnetic control system, and moving the sample to a target position by utilizing the magnetic field.
8. The method of claim 7, wherein the biological sample comprises at least one of biological cells, biological macromolecules, microparticles, and/or the non-biological particles comprise micro-nanoparticles in an environmental sample;
preferably, the biological cells comprise protozoa and/or bacteria, preferably at least one living cell comprising ciliates, cyanobacteria or magnetotactic bacteria.
9. The method according to at least one of the claims 6 to 8, characterized in that the method further comprises subjecting the sample obtained by the separation to an experimental analysis,
preferably, the experimental analysis comprises at least one of transmission electron microscopy, scanning electron microscopy and/or DNA sequencing.
10. Use of a portable microscope device as claimed in any one of claims 1 to 5 or a method as claimed in any one of claims 6 to 9 in biomedical, environmental and earth science fields, preferably for field in situ experimental research.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311424439.2A CN117539043A (en) | 2023-10-30 | 2023-10-30 | Portable microscope device for separating biological samples or non-biological particles and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311424439.2A CN117539043A (en) | 2023-10-30 | 2023-10-30 | Portable microscope device for separating biological samples or non-biological particles and application |
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| Publication Number | Publication Date |
|---|---|
| CN117539043A true CN117539043A (en) | 2024-02-09 |
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ID=89787142
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311424439.2A Pending CN117539043A (en) | 2023-10-30 | 2023-10-30 | Portable microscope device for separating biological samples or non-biological particles and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117539043A (en) |
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2023
- 2023-10-30 CN CN202311424439.2A patent/CN117539043A/en active Pending
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